NEFM cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFM
Entrez Gene ID	104488650
Full Name	neurofilament medium
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010141276.1	XP_010139578.1	neurofilament medium polypeptide

Pathways from BioSystems
Gene Ontology

The following NEFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu40076	XM_010141276.1 Latest version!	Buceros rhinoceros silvestris neurofilament, medium polypeptide (NEFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFM ( XM_010141276.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010139578.1 Buceros rhinoceros silvestris neurofilament, medium polypeptide (NEFM), mRNA.

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CloneID	OBu40076
Clone ID Related Accession (Same CDS sequence)	XM_010141276.1
Accession Version	XM_010141276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	neurofilament medium polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010425815.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACGTC ACTTGAGGGA ATACCAGGAC CTCCTCAATG TCAAGATGGC CCTGGATATT 
GAAATTGCTG CATACAGGAA GCTACTGGAA GGGGAAGAGA CAAGATTCAG TGCCTTCTCT 
GGAAGCATTA CTGGACCCAT ATTCACGCAC AGACAACCAT CTGTCACAAT AGCATCCACT 
AAAATTCAGA AAACAAAAAT TGAACCACCA AAGCTGAAGG TCCAGCACAA GTTTGTAGAA 
GAAATCATTG AAGAGACAAA GGTAGAGGAT GAAAAGTCTG AAATGGAAGA TGCCCTAGCA 
GCTATCGCGG AAGAAATGGC AGCCAAAGCC CAGCAAGAAG AACAGGAGGA AGAAAAAGCA 
GAAGCAGCTG CAGAGGAAGA AATTGTTTCC GAGAAGGCTG CTGCAGAAAA AGCAGCTGCA 
CCTGAGGAAG AAAAGGAGGA AGAGGAAGCA GAGGAGGAAG AAGCTGCAAA ATCAGATGCA 
GCAGAGGAAG GAGGTTCTGA AAAAGAAGAA ATAGAAGAAA AGGAAGAAGA GAAGGAGGCT 
GAAGAGGAGG AAGCTGAGGC CAAGGGCAAA GCTGAAGAGG CAGCAAAGGT AGAGAAGGTC 
AAAACGCCTC CCTCCAAGTC ACCCCCTAAA TCCCCCCCTA AATCGCCTGT GACGGAGGCG 
GCCAAGGCTG CCCAGAAAGA AGCAGCAAAG GAGCAGAAAG TGGAGAAAGG TGGTGAGAAA 
CCAGCCAAGG AGGAAGAGAA AGCAGCATCT CCGGAGAAGC CAGCGACACC AAAGGTAACC 
TCTCCAGAAA AGGCAGCAAG CCCGGAGAAG CCTGCGACAC CGGAGAAGCC TGCAACCCCT 
GAGAAAGCAG TGACCCCCGA GAAGGCGGCG ACGCCGGAGA AGGCGGTGAC CCCCGAAAAG 
CCCCGCACCC CAGAAAAGGC AGTGACCCCT GAAAAGCCCC GCTCTCCAGA GAAGCCCGTG 
ACTCCGGAGA AGCCCCGCTC TCCAGAGAAG CCGGCGTCCC CGGTCAAAGA TGGAAAGGCC 
GTGGTGGAGA CCATCACTGT CACAAAGGTA ACAAAAATTA GTGCAGAGGT AGAGAAGGAG 
TCCAGGAAAG AAGACATTGC GGTGAACGGT GAGGTGGAGG AGAAAAAGGA AGCAGAGGCA 
TCCAAAGAGA AGGAGGTTGA GGAGGAAGAC AAGGGTGTTG TCACTAACGG CCTAGATGTG 
AGCCCGACTG ATGATAAGGG TGAGAAAATT GTAGTAACCA AAAAAGCAGA GAAAATCTCT 
AGTGAAGGTG GGGACAGTAC AACCACATAC ATCACGAAGT CAGTGACAGT CACTCAGAAG 
GTAGAGGAAC ACGAAGAAAG CTTTGAGGAG AAATTAGTGT CCACCAAGAA AGTGGAGAAG 
GTTACTTCAC ATGCTGTAGT AAAAGAGATT AAAGAGGCAG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010139578.1
CDS	55..1479
Translation	MARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSAFSGSITGPIFTHRQPSVTIASTKIQKTKIEPPKLKVQHKFVEEIIEETKVEDEKSEMEDALAAIAEEMAAKAQQEEQEEEKAEAAAEEEIVSEKAAAEKAAAPEEEKEEEEAEEEEAAKSDAAEEGGSEKEEIEEKEEEKEAEEEEAEAKGKAEEAAKVEKVKTPPSKSPPKSPPKSPVTEAAKAAQKEAAKEQKVEKGGEKPAKEEEKAASPEKPATPKVTSPEKAASPEKPATPEKPATPEKAVTPEKAATPEKAVTPEKPRTPEKAVTPEKPRSPEKPVTPEKPRSPEKPASPVKDGKAVVETITVTKVTKISAEVEKESRKEDIAVNGEVEEKKEAEASKEKEVEEEDKGVVTNGLDVSPTDDKGEKIVVTKKAEKISSEGGDSTTTYITKSVTVTQKVEEHEESFEEKLVSTKKVEKVTSHAVVKEIKEAE

Target ORF information:

RefSeq Version	XM_010141276.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris neurofilament, medium polypeptide (NEFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010141276.1

ORF Insert Sequence:

ATGGCACGTC ACTTGAGGGA ATACCAGGAC CTCCTCAATG TCAAGATGGC CCTGGATATT 
GAAATTGCTG CATACAGGAA GCTACTGGAA GGGGAAGAGA CAAGATTCAG TGCCTTCTCT 
GGAAGCATTA CTGGACCCAT ATTCACGCAC AGACAACCAT CTGTCACAAT AGCATCCACT 
AAAATTCAGA AAACAAAAAT TGAACCACCA AAGCTGAAGG TCCAGCACAA GTTTGTAGAA 
GAAATCATTG AAGAGACAAA GGTAGAGGAT GAAAAGTCTG AAATGGAAGA TGCCCTAGCA 
GCTATCGCGG AAGAAATGGC AGCCAAAGCC CAGCAAGAAG AACAGGAGGA AGAAAAAGCA 
GAAGCAGCTG CAGAGGAAGA AATTGTTTCC GAGAAGGCTG CTGCAGAAAA AGCAGCTGCA 
CCTGAGGAAG AAAAGGAGGA AGAGGAAGCA GAGGAGGAAG AAGCTGCAAA ATCAGATGCA 
GCAGAGGAAG GAGGTTCTGA AAAAGAAGAA ATAGAAGAAA AGGAAGAAGA GAAGGAGGCT 
GAAGAGGAGG AAGCTGAGGC CAAGGGCAAA GCTGAAGAGG CAGCAAAGGT AGAGAAGGTC 
AAAACGCCTC CCTCCAAGTC ACCCCCTAAA TCCCCCCCTA AATCGCCTGT GACGGAGGCG 
GCCAAGGCTG CCCAGAAAGA AGCAGCAAAG GAGCAGAAAG TGGAGAAAGG TGGTGAGAAA 
CCAGCCAAGG AGGAAGAGAA AGCAGCATCT CCGGAGAAGC CAGCGACACC AAAGGTAACC 
TCTCCAGAAA AGGCAGCAAG CCCGGAGAAG CCTGCGACAC CGGAGAAGCC TGCAACCCCT 
GAGAAAGCAG TGACCCCCGA GAAGGCGGCG ACGCCGGAGA AGGCGGTGAC CCCCGAAAAG 
CCCCGCACCC CAGAAAAGGC AGTGACCCCT GAAAAGCCCC GCTCTCCAGA GAAGCCCGTG 
ACTCCGGAGA AGCCCCGCTC TCCAGAGAAG CCGGCGTCCC CGGTCAAAGA TGGAAAGGCC 
GTGGTGGAGA CCATCACTGT CACAAAGGTA ACAAAAATTA GTGCAGAGGT AGAGAAGGAG 
TCCAGGAAAG AAGACATTGC GGTGAACGGT GAGGTGGAGG AGAAAAAGGA AGCAGAGGCA 
TCCAAAGAGA AGGAGGTTGA GGAGGAAGAC AAGGGTGTTG TCACTAACGG CCTAGATGTG 
AGCCCGACTG ATGATAAGGG TGAGAAAATT GTAGTAACCA AAAAAGCAGA GAAAATCTCT 
AGTGAAGGTG GGGACAGTAC AACCACATAC ATCACGAAGT CAGTGACAGT CACTCAGAAG 
GTAGAGGAAC ACGAAGAAAG CTTTGAGGAG AAATTAGTGT CCACCAAGAA AGTGGAGAAG 
GTTACTTCAC ATGCTGTAGT AAAAGAGATT AAAGAGGCAG AATAA