NUB1 cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	NUB1
Entrez Gene ID	104488134
Full Name	negative regulator of ubiquitin like proteins 1
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010133604.1	XP_010131906.1	NEDD8 ultimate buster 1

Pathways from BioSystems
Gene Ontology

The following NUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu36600	XM_010133604.1 Latest version!	Buceros rhinoceros silvestris negative regulator of ubiquitin-like proteins 1 (NUB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUB1 ( XM_010133604.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010131906.1 Buceros rhinoceros silvestris negative regulator of ubiquitin-like proteins 1 (NUB1), mRNA.

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CloneID	OBu36600
Clone ID Related Accession (Same CDS sequence)	XM_010133604.1
Accession Version	XM_010133604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	NEDD8 ultimate buster 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010405153.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAA AGAAGTATTT AATAGCAAAA CTGACAAGTT GCCTAAGAGA GGACAAAATT 
CAGCTATGGA AACCACCATA TACAAATGAA AAAAAAGAAG CTGGTAAAGA GATGAAGGAG 
CTTGTACAGA AATATTCATC CAAGCTAAAT ATCAATGAGA ACGATACAGA GAATATGCTT 
GAAGAAATCC GGTGTAAAGC AATTGAGCGT GGAACAGGAA ATGAAAATTT TAAAGTGACT 
GGGATTGCAA GACTTGATAT ATATCTGCCT CGTAGAAAAA GCAGAAAAAT CCCACTGGAA 
ACTAACCTGT TCATCACAGG CAAGGAGCTG AGATCACAGA TAGCACAGGA ACATGCATTA 
AAAGAAAATG CTATCAAAAT AATCGTCAAT AAGAAGCAGC TTGACCTGGG CAAAACCCTT 
GAAGACCAGG GCGTCACACA CAATGCAAAA GTCATGGTAC TTCAGCTGGA ACAGAGCGAC 
GAGGAAACCA AAAGAAAAGT TCAGGAAGAA GAACTTCAAT GCAAGAAAGA AAAAGAAATA 
AATGACAAGA TGCAAAGAAC CAAGAAGGGT TTGGAAATTC TATCAGAGAG AGAGGAGTAC 
TTGGACCCAG ATTCTGTTCC ATATCTAGAT ATAGCTAATC AGACTGGAAG GTCAATCGAG 
ATTCCTCCTC AAGCTAAAAA AGCGCTTGTG CTGGCCATGG GTTACCATGA GAAGGGCAGA 
GCTTTAATGA AGAAGAAAGA ATATGAAATA GCACTGCCGT ATTTGTTGGA TGCTGACAAA 
CACTTCTGCG AGTGTAGCGC TGAGCTGCTG AACACCGTTG ATAACTACGC GGTGCTGCAG 
CTGGATATAG TGTGGTGCTA CTTCCGCCTG GAGCAGCTGG ACTGCCTGGA TGACGCAGAG 
AAAAAGCTGT CCACAGCTCA CAGATGCTTC CAGAGGTGCT ATGGGGAGAA CCATGAGAGG 
CTGATTGATA TCAAAGGAAG CTATGGCCGT GAGAAGGTTT TGTTTCTACG CCTCTACCTC 
CTCCAAGGGA TAGGACACTA CCACAGTGGC AGGGAAAAGG AGGCTGCTGA GTATATTCAG 
AAGGCATCTC GTTTATATGA AGAGCTGTCC GTAGATCCTG ATAAAGTTGA TCGCCTGTCG 
CTCCTGGGAT TCTCTGAACA GGAAGCTCGC CTTGCCCTGC GAGCGTGCCA TGGGGATGTG 
GAGCATGCTG CCAATCTTAT CACCAACAGG AGAGAGGAAA AGGCACAGAT AAGGAGGGAG 
GAAAGAGCTA AAAGACAGCA GCGAATGGAA GATATAAATA CCTTGAAAAG CATGGGCTAT 
TCGGAGAGAG CCGCTCAGAT AGCGCTTCAC AATACACAAG GAAACCTGGA CCAAGCCTTT 
AAGTTTATTC TTGACAATCC GGAGTTACTG TTGGAAGATG ATGATGATGA TGATCCTGTG 
GCCATGGACC AGTTTCAGGT TTCCCAGGAA AGTGTTGATC AACTGATGTA CATGGGTTTC 
AGCCGTGAGT CAGCAGAGCA AGCTTTAAAG GTGTTTAAAG GCAACATCCA CTTAGCTTCC 
CAAACCCTTG CTCATTACGG GGGAGTTCTT CCTGCATCAC TGCAGCTGTC TCCAGAAGGG 
TCCAGTCCAT CAGAAGAAAC GGCTTCATCC AAAGACTCGC TGACAGAGTC TGCAGGTTCC 
TCTAGTTCAC CAACAGATGA AGACATGGAG ACCGATGCCG TCAATGAAAT CATAGAGGAT 
ATTCCTGAGC ACGAAGAGGA TTATCTGGAT TTAACGCTGG AGGAAGAGGA GCAGATCATC 
CATGAGTACT TATCCTATAT ACAAGTCCCA CAGCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010131906.1
CDS	2..1840
Translation	MAQKKYLIAKLTSCLREDKIQLWKPPYTNEKKEAGKEMKELVQKYSSKLNINENDTENMLEEIRCKAIERGTGNENFKVTGIARLDIYLPRRKSRKIPLETNLFITGKELRSQIAQEHALKENAIKIIVNKKQLDLGKTLEDQGVTHNAKVMVLQLEQSDEETKRKVQEEELQCKKEKEINDKMQRTKKGLEILSEREEYLDPDSVPYLDIANQTGRSIEIPPQAKKALVLAMGYHEKGRALMKKKEYEIALPYLLDADKHFCECSAELLNTVDNYAVLQLDIVWCYFRLEQLDCLDDAEKKLSTAHRCFQRCYGENHERLIDIKGSYGREKVLFLRLYLLQGIGHYHSGREKEAAEYIQKASRLYEELSVDPDKVDRLSLLGFSEQEARLALRACHGDVEHAANLITNRREEKAQIRREERAKRQQRMEDINTLKSMGYSERAAQIALHNTQGNLDQAFKFILDNPELLLEDDDDDDPVAMDQFQVSQESVDQLMYMGFSRESAEQALKVFKGNIHLASQTLAHYGGVLPASLQLSPEGSSPSEETASSKDSLTESAGSSSSPTDEDMETDAVNEIIEDIPEHEEDYLDLTLEEEEQIIHEYLSYIQVPQH

Target ORF information:

RefSeq Version	XM_010133604.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris negative regulator of ubiquitin-like proteins 1 (NUB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010133604.1

ORF Insert Sequence:

ATGGCACAAA AGAAGTATTT AATAGCAAAA CTGACAAGTT GCCTAAGAGA GGACAAAATT 
CAGCTATGGA AACCACCATA TACAAATGAA AAAAAAGAAG CTGGTAAAGA GATGAAGGAG 
CTTGTACAGA AATATTCATC CAAGCTAAAT ATCAATGAGA ACGATACAGA GAATATGCTT 
GAAGAAATCC GGTGTAAAGC AATTGAGCGT GGAACAGGAA ATGAAAATTT TAAAGTGACT 
GGGATTGCAA GACTTGATAT ATATCTGCCT CGTAGAAAAA GCAGAAAAAT CCCACTGGAA 
ACTAACCTGT TCATCACAGG CAAGGAGCTG AGATCACAGA TAGCACAGGA ACATGCATTA 
AAAGAAAATG CTATCAAAAT AATCGTCAAT AAGAAGCAGC TTGACCTGGG CAAAACCCTT 
GAAGACCAGG GCGTCACACA CAATGCAAAA GTCATGGTAC TTCAGCTGGA ACAGAGCGAC 
GAGGAAACCA AAAGAAAAGT TCAGGAAGAA GAACTTCAAT GCAAGAAAGA AAAAGAAATA 
AATGACAAGA TGCAAAGAAC CAAGAAGGGT TTGGAAATTC TATCAGAGAG AGAGGAGTAC 
TTGGACCCAG ATTCTGTTCC ATATCTAGAT ATAGCTAATC AGACTGGAAG GTCAATCGAG 
ATTCCTCCTC AAGCTAAAAA AGCGCTTGTG CTGGCCATGG GTTACCATGA GAAGGGCAGA 
GCTTTAATGA AGAAGAAAGA ATATGAAATA GCACTGCCGT ATTTGTTGGA TGCTGACAAA 
CACTTCTGCG AGTGTAGCGC TGAGCTGCTG AACACCGTTG ATAACTACGC GGTGCTGCAG 
CTGGATATAG TGTGGTGCTA CTTCCGCCTG GAGCAGCTGG ACTGCCTGGA TGACGCAGAG 
AAAAAGCTGT CCACAGCTCA CAGATGCTTC CAGAGGTGCT ATGGGGAGAA CCATGAGAGG 
CTGATTGATA TCAAAGGAAG CTATGGCCGT GAGAAGGTTT TGTTTCTACG CCTCTACCTC 
CTCCAAGGGA TAGGACACTA CCACAGTGGC AGGGAAAAGG AGGCTGCTGA GTATATTCAG 
AAGGCATCTC GTTTATATGA AGAGCTGTCC GTAGATCCTG ATAAAGTTGA TCGCCTGTCG 
CTCCTGGGAT TCTCTGAACA GGAAGCTCGC CTTGCCCTGC GAGCGTGCCA TGGGGATGTG 
GAGCATGCTG CCAATCTTAT CACCAACAGG AGAGAGGAAA AGGCACAGAT AAGGAGGGAG 
GAAAGAGCTA AAAGACAGCA GCGAATGGAA GATATAAATA CCTTGAAAAG CATGGGCTAT 
TCGGAGAGAG CCGCTCAGAT AGCGCTTCAC AATACACAAG GAAACCTGGA CCAAGCCTTT 
AAGTTTATTC TTGACAATCC GGAGTTACTG TTGGAAGATG ATGATGATGA TGATCCTGTG 
GCCATGGACC AGTTTCAGGT TTCCCAGGAA AGTGTTGATC AACTGATGTA CATGGGTTTC 
AGCCGTGAGT CAGCAGAGCA AGCTTTAAAG GTGTTTAAAG GCAACATCCA CTTAGCTTCC 
CAAACCCTTG CTCATTACGG GGGAGTTCTT CCTGCATCAC TGCAGCTGTC TCCAGAAGGG 
TCCAGTCCAT CAGAAGAAAC GGCTTCATCC AAAGACTCGC TGACAGAGTC TGCAGGTTCC 
TCTAGTTCAC CAACAGATGA AGACATGGAG ACCGATGCCG TCAATGAAAT CATAGAGGAT 
ATTCCTGAGC ACGAAGAGGA TTATCTGGAT TTAACGCTGG AGGAAGAGGA GCAGATCATC 
CATGAGTACT TATCCTATAT ACAAGTCCCA CAGCATTAA