WASHC4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	WASHC4
Entrez Gene ID	102416532
Full Name	WASH complex subunit 4
Synonyms	KIAA1033
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006056596.1	XP_006056658.1	WASH complex subunit 7
XM_006056596.2	XP_006056658.1	WASH complex subunit 4

Pathways from BioSystems
Gene Ontology

The following WASHC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WASHC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu13689	XM_006056596.1 Latest version!	Bubalus bubalis KIAA1033 ortholog (KIAA1033), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu64445	XM_006056596.2 Latest version!	Bubalus bubalis WASH complex subunit 4 (WASHC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WASHC4 ( XM_006056596.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056658.1 Bubalus bubalis KIAA1033 ortholog (KIAA1033), mRNA.

Price & Availability↑

CloneID	OBu13689
Clone ID Related Accession (Same CDS sequence)	XM_006056596.1
Accession Version	XM_006056596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	WASH complex subunit 7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784764.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTAGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA GCTTAAGAAT TATGGGAAAT TTCTCGAGGA GTATACCTCT 
CAATTGAGAA GAATTGAGGA CGCACTGGAT GACTCAATTG GAGATGTTTG GGATTTCAAT 
CTTGATCCTA TAGCCCTAAA GCTTTTGCCT TATGAACAGT CTTCCCTTTT GGAACTCATC 
AAGACTGAAA ACAAGGTCCT AAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAATATGA GGCTGAAACT AAGTTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTAGAA GGTGATTGTC AAATTCAAAT GGGGAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAT 
GTAGTCCATC AGTTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAA 
ACAACTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACGCTGG ATGAAATTAT TGATAATCAC ATCACATTAA AAGACCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAGT TTGGAATTCA GGAAGAAAAG 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAC TCTCTCAATG GAGGAGTATC TGTGTCGAAA 
AATAGCACTT TTGCTGAAGA ATTTGCACAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAGACTTG GAGAACCTTC TGAAATTGAT CAGAGAGACA AGTATGTGGG AGTTTGTGGA 
CTCTCTGTAT TGCACTTTCA AATTTTTCGA ACCATTGATA AAAAGTTTTA CAAGTCTTTA 
TTGGACATTT GCAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTGTGGACAA AAAAAGTCTT 
CAGGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCGCAGTC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGT TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCC GAAGACCTCA CCAGTAGATG TAATGTTTTT 
ATACAGGGCT TTTTATATGC ATATAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCCA TGCAAAAGCC AATGACCAAA ACATCAGTTA AAGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCTATAGA GCATATGTTC TACAGGAGAA GCATGGTTGT GGCTGATTCA 
GTCTCACATA TAACACAGCA CCTTCAGCAT CAGGCTCTTA ATTCTATTTC TGTGGCCAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGT GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAAGC AGCGACGACT TATTGTTTCT 
TTGGCACTGA GTGTGGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAGCT CTTTCCACTT 
CAAGTAGTCA TGAAGAAACT GGATCTTATC AGTGAACTCC GAGAAAGAGT CCAGACACAG 
TGTGACTGTT GTTTTCTGTA CTGGCATCGA GCTGTCTTCC CAATTTATTT AGATGACGTA 
TATGAAAATG CTGTTGATGC AGCCAGACTA CATTACATGT TCAGTGCTTT ACGTGACTGT 
GTGCCTGCCA TGATGCATGC AAGGCATTTG GAGTCCTATG AAGTACTTCT GGATTGCTAT 
GACAAGGAAA TCATGGAAAT TTTAAATGAG CATTTGCTGG ACAAGTTGTG CAAAGAAATC 
GAGAAGGACC TGCGACTTTC TGTGCATACT CATTTAAAGC TGGATGACAG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTCTGA ATCCAATTCG GTTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT TTACAATCTA 
ACGACAGTAG CTCTTCATGA CTGGGCTACT TACAGTGAAA TGAGAAATTT AGCTACTCAG 
CGTTACGGAT TGGTTATGAC AGAGGCCCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTCTTGG AAATTATGAG AAACATTCAT ATATTTGTAT CTCGATACCT CTATAATCTC 
AACAATCAAA TTTTTATTGA ACGAACAAGT AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTCAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTTA GCCAATTTAT GTATGATGAA 
CATATCAAAT CCAGATTGAT TAAAGATATT CGGTTTTTCA GGGAAATCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAATC GAGGCATCAG AAAACTTGGA 
ATAACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AGCTCATCAG CCAAATTGGT 
AATGCTATGG GCTATGTACG AATGATAAGA TCTGGTGGCC TTCATTGTAG CAGCAATGCC 
ATTAGATTTG TACCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT CAAAGAAGAA 
GGTCTTGCAG AAGAAACGTT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACAGAATAT TTCAAAATGC TTGTGGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTC CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGT TGCAAGGAGA AGTTGAATAA AAAAAATAAA 
ATTGGAGCTG CCTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TCTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAATAC 
CTGAAGGAGA TGAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAACTAG TCAGGATGAA 
AAACTACTAC AAACCATGAA TCTCACTCAG AAACGACTGG ATGTTTATCT ACAGGAATTT 
GAATTGCTGT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCTGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAAGAGG CTAAAACAAG CAATGGAGAC 
CTGTCCGACA GCACGGTGTC TGCAGATCCT GTTGTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056658.1
CDS	85..3606
Translation	MAVETLSPDWEFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDSSMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEARLGEPSEIDQRDKYVGVCGLSVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLVDKKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTSRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALNSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDCCFLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESYEVLLDCYDKEIMEILNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFERAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAMGYVRMIRSGGLHCSSNAIRFVPDLEDIVNFEELVKEEGLAEETLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAFTDDGFAMGVAYILKLLDQYQEFDSLHWFQSVREKYLKEMRAVAKQQNVQSTSQDEKLLQTMNLTQKRLDVYLQEFELLYFSLSSARIFFRADKTAAEENQEKKEKEEEAKTSNGDLSDSTVSADPVVK

Target ORF information:

RefSeq Version	XM_006056596.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis KIAA1033 ortholog (KIAA1033), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056596.1

ORF Insert Sequence:

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTAGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA GCTTAAGAAT TATGGGAAAT TTCTCGAGGA GTATACCTCT 
CAATTGAGAA GAATTGAGGA CGCACTGGAT GACTCAATTG GAGATGTTTG GGATTTCAAT 
CTTGATCCTA TAGCCCTAAA GCTTTTGCCT TATGAACAGT CTTCCCTTTT GGAACTCATC 
AAGACTGAAA ACAAGGTCCT AAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAATATGA GGCTGAAACT AAGTTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTAGAA GGTGATTGTC AAATTCAAAT GGGGAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAT 
GTAGTCCATC AGTTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAA 
ACAACTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACGCTGG ATGAAATTAT TGATAATCAC ATCACATTAA AAGACCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAGT TTGGAATTCA GGAAGAAAAG 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAC TCTCTCAATG GAGGAGTATC TGTGTCGAAA 
AATAGCACTT TTGCTGAAGA ATTTGCACAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAGACTTG GAGAACCTTC TGAAATTGAT CAGAGAGACA AGTATGTGGG AGTTTGTGGA 
CTCTCTGTAT TGCACTTTCA AATTTTTCGA ACCATTGATA AAAAGTTTTA CAAGTCTTTA 
TTGGACATTT GCAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTGTGGACAA AAAAAGTCTT 
CAGGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCGCAGTC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGT TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCC GAAGACCTCA CCAGTAGATG TAATGTTTTT 
ATACAGGGCT TTTTATATGC ATATAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCCA TGCAAAAGCC AATGACCAAA ACATCAGTTA AAGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCTATAGA GCATATGTTC TACAGGAGAA GCATGGTTGT GGCTGATTCA 
GTCTCACATA TAACACAGCA CCTTCAGCAT CAGGCTCTTA ATTCTATTTC TGTGGCCAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGT GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAAGC AGCGACGACT TATTGTTTCT 
TTGGCACTGA GTGTGGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAGCT CTTTCCACTT 
CAAGTAGTCA TGAAGAAACT GGATCTTATC AGTGAACTCC GAGAAAGAGT CCAGACACAG 
TGTGACTGTT GTTTTCTGTA CTGGCATCGA GCTGTCTTCC CAATTTATTT AGATGACGTA 
TATGAAAATG CTGTTGATGC AGCCAGACTA CATTACATGT TCAGTGCTTT ACGTGACTGT 
GTGCCTGCCA TGATGCATGC AAGGCATTTG GAGTCCTATG AAGTACTTCT GGATTGCTAT 
GACAAGGAAA TCATGGAAAT TTTAAATGAG CATTTGCTGG ACAAGTTGTG CAAAGAAATC 
GAGAAGGACC TGCGACTTTC TGTGCATACT CATTTAAAGC TGGATGACAG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTCTGA ATCCAATTCG GTTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT TTACAATCTA 
ACGACAGTAG CTCTTCATGA CTGGGCTACT TACAGTGAAA TGAGAAATTT AGCTACTCAG 
CGTTACGGAT TGGTTATGAC AGAGGCCCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTCTTGG AAATTATGAG AAACATTCAT ATATTTGTAT CTCGATACCT CTATAATCTC 
AACAATCAAA TTTTTATTGA ACGAACAAGT AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTCAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTTA GCCAATTTAT GTATGATGAA 
CATATCAAAT CCAGATTGAT TAAAGATATT CGGTTTTTCA GGGAAATCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAATC GAGGCATCAG AAAACTTGGA 
ATAACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AGCTCATCAG CCAAATTGGT 
AATGCTATGG GCTATGTACG AATGATAAGA TCTGGTGGCC TTCATTGTAG CAGCAATGCC 
ATTAGATTTG TACCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT CAAAGAAGAA 
GGTCTTGCAG AAGAAACGTT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACAGAATAT TTCAAAATGC TTGTGGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTC CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGT TGCAAGGAGA AGTTGAATAA AAAAAATAAA 
ATTGGAGCTG CCTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TCTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCACTT CACTGGTTCC AGTCTGTTAG AGAGAAATAC 
CTGAAGGAGA TGAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAACTAG TCAGGATGAA 
AAACTACTAC AAACCATGAA TCTCACTCAG AAACGACTGG ATGTTTATCT ACAGGAATTT 
GAATTGCTGT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCTGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAAGAGG CTAAAACAAG CAATGGAGAC 
CTGTCCGACA GCACGGTGTC TGCAGATCCT GTTGTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WASHC4 ( XM_006056596.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006056658.1 Bubalus bubalis WASH complex subunit 4 (WASHC4), mRNA.

Price & Availability↑

CloneID	OBu64445
Clone ID Related Accession (Same CDS sequence)	XM_006056596.2
Accession Version	XM_006056596.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	WASH complex subunit 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006056596.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTAGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA GCTTAAGAAT TATGGGAAAT TTCTCGAGGA GTATACCTCT 
CAATTGAGAA GAATTGAGGA CGCACTGGAT GACTCAATTG GAGATGTTTG GGATTTCAAT 
CTTGATCCTA TAGCCCTAAA GCTTTTGCCT TATGAACAGT CTTCCCTTTT GGAACTCATC 
AAGACTGAAA ACAAGGTCCT AAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAATATGA GGCTGAAACT AAGTTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTAGAA GGTGATTGTC AAATTCAAAT GGGGAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAT 
GTAGTCCATC AGTTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAA 
ACAACTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACGCTGG ATGAAATTAT TGATAATCAC ATCACATTAA AAGACCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAGT TTGGAATTCA GGAAGAAAAG 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAC TCTCTCAATG GAGGAGTATC TGTGTCGAAA 
AATAGCACTT TTGCTGAAGA ATTTGCACAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAGACTTG GAGAACCTTC TGAAATTGAT CAGAGAGACA AGTATGTGGG AGTTTGTGGA 
CTCTCTGTAT TGCACTTTCA AATTTTTCGA ACCATTGATA AAAAGTTTTA CAAGTCTTTA 
TTGGACATTT GCAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTGTGGACAA AAAAAGTCTT 
CAGGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCGCAGTC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGT TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCC GAAGACCTCA CCAGTAGATG TAATGTTTTT 
ATACAGGGCT TTTTATATGC ATATAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCCA TGCAAAAGCC AATGACCAAA ACATCAGTTA AAGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCTATAGA GCATATGTTC TACAGGAGAA GCATGGTTGT GGCTGATTCA 
GTCTCACATA TAACACAGCA CCTTCAGCAT CAGGCTCTTA ATTCTATTTC TGTGGCCAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGT GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAAGC AGCGACGACT TATTGTTTCT 
TTGGCACTGA GTGTGGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAGCT CTTTCCACTT 
CAAGTAGTCA TGAAGAAACT GGATCTTATC AGTGAACTCC GAGAAAGAGT CCAGACACAG 
TGTGACTGTT GTTTTCTGTA CTGGCATCGA GCTGTCTTCC CAATTTATTT AGATGACGTA 
TATGAAAATG CTGTTGATGC AGCCAGACTA CATTACATGT TCAGTGCTTT ACGTGACTGT 
GTGCCTGCCA TGATGCATGC AAGGCATTTG GAGTCCTATG AAGTACTTCT GGATTGCTAT 
GACAAGGAAA TCATGGAAAT TTTAAATGAG CATTTGCTGG ACAAGTTGTG CAAAGAAATC 
GAGAAGGACC TGCGACTTTC TGTGCATACT CATTTAAAGC TGGATGACAG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTCTGA ATCCAATTCG GTTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT TTACAATCTA 
ACGACAGTAG CTCTTCATGA CTGGGCTACT TACAGTGAAA TGAGAAATTT AGCTACTCAG 
CGTTACGGAT TGGTTATGAC AGAGGCCCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTCTTGG AAATTATGAG AAACATTCAT ATATTTGTAT CTCGATACCT CTATAATCTC 
AACAATCAAA TTTTTATTGA ACGAACAAGT AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTCAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTTA GCCAATTTAT GTATGATGAA 
CATATCAAAT CCAGATTGAT TAAAGATATT CGGTTTTTCA GGGAAATCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAATC GAGGCATCAG AAAACTTGGA 
ATAACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AGCTCATCAG CCAAATTGGT 
AATGCTATGG GCTATGTACG AATGATAAGA TCTGGTGGCC TTCATTGTAG CAGCAATGCC 
ATTAGATTTG TACCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT CAAAGAAGAA 
GGTCTTGCAG AAGAAACGTT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACAGAATAT TTCAAAATGC TTGTGGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTC CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGT TGCAAGGAGA AGTTGAATAA AAAAAATAAA 
ATTGGAGCTG CCTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TCTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCGCTT CACTGGTTCC AGTCTGTTAG AGAGAAATAC 
CTGAAGGAGA TGAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAACTAG TCAGGATGAA 
AAACTACTAC AAACCATGAA TCTCACTCAG AAACGACTGG ATGTTTATCT ACAGGAATTT 
GAATTGCTGT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCTGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAAGAGG CTAAAACAAG CAATGGAGAC 
CTGTCCGACA GCACGGTGTC TGCAGATCCT GTTGTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006056658.1
CDS	89..3610
Translation	MAVETLSPDWEFDRVDDGSQKIHAEVQLKNYGKFLEEYTSQLRRIEDALDDSIGDVWDFNLDPIALKLLPYEQSSLLELIKTENKVLNKVITVYAALCCEIKKLKYEAETKFYNGLLFYGEGATDSSMVEGDCQIQMGRFISFLQELSCFVTRCYEVVMNVVHQLAALYISNKIAPKIIETTGVHFQTMYEHLGELLTVLLTLDEIIDNHITLKDHWTMYKRLLKSVHHNPSKFGIQEEKLKPFEKFLLKLEGQLLDGMIFQACIEQQFDSLNGGVSVSKNSTFAEEFAHSIRSIFANVEARLGEPSEIDQRDKYVGVCGLSVLHFQIFRTIDKKFYKSLLDICKKVPAITLTANIIWFPDNFLIQKIPAAAKLVDKKSLQAIKIHRDTFLQQKAQSLTKDVQSYYVFVSSWMMKMESILSKEQRMDKFAEDLTSRCNVFIQGFLYAYSISTIIKTTMNLYMSMQKPMTKTSVKALCRLVELLKAIEHMFYRRSMVVADSVSHITQHLQHQALNSISVAKKRVISDKKYSEQRLDVLSALVLAENTLNGPSTKQRRLIVSLALSVGTQMKTFKDEELFPLQVVMKKLDLISELRERVQTQCDCCFLYWHRAVFPIYLDDVYENAVDAARLHYMFSALRDCVPAMMHARHLESYEVLLDCYDKEIMEILNEHLLDKLCKEIEKDLRLSVHTHLKLDDRNPFKVGMKDLALFFSLNPIRFFNRFIDIRAYVTHYLDKTFYNLTTVALHDWATYSEMRNLATQRYGLVMTEAHLPSQTLEQGLDVLEIMRNIHIFVSRYLYNLNNQIFIERTSNNKHLNTINIRHIANSIRTHGTGIMNTTVNFTYQFLKKKFYIFSQFMYDEHIKSRLIKDIRFFREIKDQNDHKYPFERAEKFNRGIRKLGITPEGQSYLDQFRQLISQIGNAMGYVRMIRSGGLHCSSNAIRFVPDLEDIVNFEELVKEEGLAEETLKAARHLDSVLSDHTRNSAEGTEYFKMLVDVFAPEFRRPKNIHLRNFYIIVPPLTLNFVEHSISCKEKLNKKNKIGAAFTDDGFAMGVAYILKLLDQYQEFDSLHWFQSVREKYLKEMRAVAKQQNVQSTSQDEKLLQTMNLTQKRLDVYLQEFELLYFSLSSARIFFRADKTAAEENQEKKEKEEEAKTSNGDLSDSTVSADPVVK

Target ORF information:

RefSeq Version	XM_006056596.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis WASH complex subunit 4 (WASHC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006056596.2

ORF Insert Sequence:

ATGGCGGTGG AGACTCTGTC CCCGGACTGG GAGTTCGACC GCGTAGACGA CGGCTCACAG 
AAAATTCATG CTGAAGTCCA GCTTAAGAAT TATGGGAAAT TTCTCGAGGA GTATACCTCT 
CAATTGAGAA GAATTGAGGA CGCACTGGAT GACTCAATTG GAGATGTTTG GGATTTCAAT 
CTTGATCCTA TAGCCCTAAA GCTTTTGCCT TATGAACAGT CTTCCCTTTT GGAACTCATC 
AAGACTGAAA ACAAGGTCCT AAACAAAGTC ATCACTGTTT ATGCTGCACT TTGTTGTGAA 
ATCAAGAAAT TAAAATATGA GGCTGAAACT AAGTTTTATA ATGGTCTCTT GTTTTATGGA 
GAAGGAGCTA CAGATTCCAG CATGGTAGAA GGTGATTGTC AAATTCAAAT GGGGAGATTT 
ATTTCATTCT TACAGGAACT GTCTTGTTTT GTTACGAGGT GCTATGAAGT GGTGATGAAT 
GTAGTCCATC AGTTGGCTGC CCTCTATATC AGTAACAAGA TTGCACCCAA AATTATAGAA 
ACAACTGGAG TTCATTTTCA GACTATGTAT GAGCACTTGG GAGAGCTGCT AACAGTTTTG 
CTTACGCTGG ATGAAATTAT TGATAATCAC ATCACATTAA AAGACCACTG GACTATGTAC 
AAAAGGTTAC TGAAATCTGT CCATCACAAT CCTTCAAAGT TTGGAATTCA GGAAGAAAAG 
CTAAAGCCAT TTGAAAAGTT CTTGCTGAAG CTAGAAGGGC AGTTACTTGA TGGAATGATA 
TTTCAGGCCT GTATAGAACA GCAGTTTGAC TCTCTCAATG GAGGAGTATC TGTGTCGAAA 
AATAGCACTT TTGCTGAAGA ATTTGCACAT AGTATTCGCT CAATTTTTGC AAATGTAGAA 
GCCAGACTTG GAGAACCTTC TGAAATTGAT CAGAGAGACA AGTATGTGGG AGTTTGTGGA 
CTCTCTGTAT TGCACTTTCA AATTTTTCGA ACCATTGATA AAAAGTTTTA CAAGTCTTTA 
TTGGACATTT GCAAGAAGGT ACCAGCCATC ACTCTAACTG CTAATATTAT TTGGTTTCCT 
GATAATTTTT TGATCCAGAA AATACCAGCA GCTGCCAAAC TTGTGGACAA AAAAAGTCTT 
CAGGCCATTA AAATACACAG AGATACTTTC CTACAGCAGA AAGCGCAGTC ACTTACCAAA 
GATGTACAGT CTTACTACGT CTTTGTGAGT TCATGGATGA TGAAAATGGA ATCTATTTTA 
TCTAAAGAGC AGAGAATGGA TAAATTTGCC GAAGACCTCA CCAGTAGATG TAATGTTTTT 
ATACAGGGCT TTTTATATGC ATATAGTATT AGTACCATTA TTAAAACCAC AATGAATCTC 
TACATGTCCA TGCAAAAGCC AATGACCAAA ACATCAGTTA AAGCATTATG CAGGCTTGTT 
GAACTTCTCA AGGCTATAGA GCATATGTTC TACAGGAGAA GCATGGTTGT GGCTGATTCA 
GTCTCACATA TAACACAGCA CCTTCAGCAT CAGGCTCTTA ATTCTATTTC TGTGGCCAAG 
AAAAGAGTAA TTTCTGACAA AAAATATAGT GAACAGCGTC TTGATGTGCT CTCTGCTCTA 
GTTTTGGCTG AAAACACTCT AAATGGACCA AGCACAAAGC AGCGACGACT TATTGTTTCT 
TTGGCACTGA GTGTGGGCAC ACAAATGAAA ACATTTAAAG ATGAAGAGCT CTTTCCACTT 
CAAGTAGTCA TGAAGAAACT GGATCTTATC AGTGAACTCC GAGAAAGAGT CCAGACACAG 
TGTGACTGTT GTTTTCTGTA CTGGCATCGA GCTGTCTTCC CAATTTATTT AGATGACGTA 
TATGAAAATG CTGTTGATGC AGCCAGACTA CATTACATGT TCAGTGCTTT ACGTGACTGT 
GTGCCTGCCA TGATGCATGC AAGGCATTTG GAGTCCTATG AAGTACTTCT GGATTGCTAT 
GACAAGGAAA TCATGGAAAT TTTAAATGAG CATTTGCTGG ACAAGTTGTG CAAAGAAATC 
GAGAAGGACC TGCGACTTTC TGTGCATACT CATTTAAAGC TGGATGACAG AAACCCTTTC 
AAAGTTGGCA TGAAAGACCT GGCCCTTTTT TTCTCTCTGA ATCCAATTCG GTTTTTCAAT 
CGTTTCATTG ACATTCGAGC TTATGTAACT CACTACCTAG ACAAGACTTT TTACAATCTA 
ACGACAGTAG CTCTTCATGA CTGGGCTACT TACAGTGAAA TGAGAAATTT AGCTACTCAG 
CGTTACGGAT TGGTTATGAC AGAGGCCCAT CTTCCTAGTC AGACTTTGGA ACAGGGCCTG 
GATGTCTTGG AAATTATGAG AAACATTCAT ATATTTGTAT CTCGATACCT CTATAATCTC 
AACAATCAAA TTTTTATTGA ACGAACAAGT AATAACAAAC ATTTGAACAC TATTAATATT 
CGGCATATTG CTAATTCAAT TCGAACACAT GGCACAGGAA TCATGAATAC AACAGTCAAT 
TTCACCTACC AGTTTTTGAA AAAGAAGTTC TATATATTTA GCCAATTTAT GTATGATGAA 
CATATCAAAT CCAGATTGAT TAAAGATATT CGGTTTTTCA GGGAAATCAA GGACCAAAAT 
GATCATAAGT ATCCTTTTGA AAGAGCAGAA AAATTCAATC GAGGCATCAG AAAACTTGGA 
ATAACCCCAG AGGGACAAAG CTACCTTGAT CAGTTCAGGC AGCTCATCAG CCAAATTGGT 
AATGCTATGG GCTATGTACG AATGATAAGA TCTGGTGGCC TTCATTGTAG CAGCAATGCC 
ATTAGATTTG TACCTGATCT TGAAGATATT GTAAATTTTG AAGAACTAGT CAAAGAAGAA 
GGTCTTGCAG AAGAAACGTT AAAAGCAGCA AGGCATTTGG ATTCAGTCCT CAGTGATCAC 
ACACGAAATT CTGCTGAAGG CACAGAATAT TTCAAAATGC TTGTGGATGT TTTTGCTCCA 
GAATTTCGAA GGCCAAAGAA TATACATCTC CGAAATTTCT ATATCATTGT TCCCCCTCTG 
ACCCTCAACT TTGTAGAGCA TTCCATTAGT TGCAAGGAGA AGTTGAATAA AAAAAATAAA 
ATTGGAGCTG CCTTTACTGA TGATGGCTTT GCCATGGGTG TGGCTTACAT TCTGAAGCTT 
TTGGATCAGT ACCAGGAATT TGACTCGCTT CACTGGTTCC AGTCTGTTAG AGAGAAATAC 
CTGAAGGAGA TGAGAGCAGT TGCTAAGCAA CAGAATGTAC AGTCAACTAG TCAGGATGAA 
AAACTACTAC AAACCATGAA TCTCACTCAG AAACGACTGG ATGTTTATCT ACAGGAATTT 
GAATTGCTGT ATTTCTCACT GAGCAGTGCA AGAATTTTCT TCAGAGCTGA CAAGACTGCG 
GCTGAAGAAA ACCAAGAAAA GAAAGAGAAA GAAGAAGAGG CTAAAACAAG CAATGGAGAC 
CTGTCCGACA GCACGGTGTC TGCAGATCCT GTTGTCAAAT GA