SEMA4G cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA4G
Entrez Gene ID	102408276
Full Name	semaphorin 4G
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006053180.2	XP_006053242.2	semaphorin-4G
XM_006053180.1	XP_006053242.1	semaphorin-4G

Pathways from BioSystems
Gene Ontology

The following SEMA4G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA4G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu10794	XM_006053180.1 Latest version!	Bubalus bubalis sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu59216	XM_006053180.2 Latest version!	Bubalus bubalis semaphorin 4G (SEMA4G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA4G ( XM_006053180.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053242.1 Bubalus bubalis sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), mRNA.

Price & Availability↑

CloneID	OBu10794
Clone ID Related Accession (Same CDS sequence)	XM_006053180.1
Accession Version	XM_006053180.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	semaphorin-4G
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGGA GGCTCTGGCC CCTCCTCCTG AGCTTCCTCA CAGCAACTGC AGTCCCTGGA 
CCCTCACTGC GGAGACCATC CAGAGAACTA GATGCCACCC CACGGATGAC CATCCCCTAT 
GAAGAGCTCT CTGGGACCCG GCACTTCAAG GGCCAAGCCC AGAACTACTC AACACTGCTG 
CTGGAGGAGG CCTCCGCAAG GCTGCTGGTG GGAGCCCGAG GTGCCCTGTT CTCTCTCAAT 
GCGCAAGACA TAGGAGATGG GACTCACAAA GAGATTCACT GGGAGGCCTC TCCAGAGATG 
CAAAGCAAAT GTCATCAAAA AGGGAAAAAC AACCAGACCG AGTGCTTCAA CCATGTGCGA 
TTCCTGCAGC GGCTCAATGC CACCCACCTC TACGCATGTG GGACTCACGC CTTCCAGCCT 
CTCTGTGCAG CCATTGATGC TGAGGCCTTC ACCTTGCCAA CCAGCTTCGA GGAGGGGAAA 
GAGAAGTGTC CTTATGACCC AGCCCGCGGC TTCACTGGCC TCATCATTGA TGGAGGCCTC 
TACACAGCCA CTCGGTATGA ATTCCGGAGC ATCCCTGACA TCCGCCGGAG CCGGCACCCA 
CACTCCCTGA GGACAGAGGA GGCACCAATG CACTGGCTCA ATGATGCTGA GTTTGTGTTC 
TCCGTCCTGG TGCGGGAGAG CCAGGCCAGT GCCGTGGGTG ACGACGACAA AGTTTACTAC 
TTCTTCACGG AGCGTGCTGC TGAGGAGGGT GCAGGCAGCT TTGCTCAGAG CCGCAGCAGT 
CACCGCGTGG CCCGTGTGGC CCGGGTGTGC AAGGGAGACC TGGGAGGGAA GAAGATCCTG 
CAGAAGAAAT GGACCTCCTT CTTGAAGGCG CGCCTCATCT GCCACATCCC ACAGTATGAG 
ACGCTGCGCA GTGTCTGCAG CCTGCAAGCT GACACCTCAG CACACACACA CTTCTATGCG 
GCCTTCACGC TGACCACACA GTGGAAGACC CTCGAGGCCT CAGCCATATG CCGCTATGAC 
CTGGCCGAGG TGCAGGCTGT CTTCGCAGGC CCCTACATGG AATACCAGGA TGGTGTCCGG 
CGCTGGGGCC GCTACGAGGG CGGGGTGCCA GAGCCCCGGC CTGGCTCGTG CATTACAGAT 
TCACTGCGAA GCCAAGGCTA CAACTCATCC CAGGACTTGC CATCTCTGGT TCTGGACTTT 
GTGAAGTTAC ACCCACTGAT GGCTCGGCCT GTAGTGCCCA CACGTGGACG GCCCCTGCTG 
CTCAAGCGCA GTGTGCGCTA CACACACCTC ACGGGGACAC CTGTCAGCAC ACCCGCCGGA 
CCCACCTATG ACCTGCTCTT TCTGGGCACA GCTGATGGCT GGATCCACAA GGCTGTGGTC 
CTGGGCTCTG GGATGCACAT TATTGAAGAG ACACAGGTGT TCAAGGATCC CCAGTCTGTG 
GAGAACCTGG TCATCTCTCT GATGCAGCAT AGCCTCTACG TGGGGGCCCC AAGTGGCGTC 
ATCCAGCTAC CACTGTCCAG CTGCTCCCGC TACCGCTCCT GCTATGACTG CATCCTGGCC 
CGGGACCCCT ACTGTGGCTG GGACCCCAGC ACCCATGCCT GCATGGTGAC CACCACCGTA 
GCCAACAGGT CCCAAAATAG CAGGACAGCA CTCATACAGG ACATGGAGAG AGGAAACCGA 
GGCTGCGAGG GCAACAGGGA CACAGGGCCA CCACCACCAC TGAAGACCCG TTCTGTGCTC 
CGGGGTGATG ATGTCCTATT GCCCTGTGAC CAGCCATCCA ACCTGGCCCG GGCCTTGTGG 
CTGCTCAATG GGAGCATGGG CCTGAGTGAT GGGCAGGATG GCTACCGCGT GGGTGTGGAT 
GGGCTGCTGG TAACAGACAC GCAGCCTGAG CACAGCGGCA ACTACGGCTG CTATGCTGAA 
GAGAATGGCC TCCGCACCCT GCTGGCCTCC TACAGCCTCA CGGTGCGGCC AGCCACTCCA 
GCCCCTGCTC CACAAGCCCC TGCCATGCCT GGGGCACAGC TGGCACCGGA TGTGCGGCTG 
CTCTACGTGT TAGCCATTGC TGCACTTGGT GGCCTCTGCC TTATCCTGGC TTCCTCCCTC 
TTCTATGTGG CCTGTCTTCG GGGAAGTGGA CGAGGGCACC GTCAAAAATA CTCACTGGGT 
CGGGCTGGCC GGGCAGGGGG CTCTGCTGTG CAGCTGCAGA CAGTCTCAGG CCAGTGTCCT 
GGAGAGGATG ATGACGGTGA TGATGGGGAA CGGGCCGGGG GCCTAGAGGG TGGCTGCCTC 
CAGATCATCC CTGGAGAGGG AACCCCAGCC CCGCCGCCAC CGCCCCCCCC ACCGCCACCA 
ACTGAGCTGA CCAATGGGTT GGTGACACTG CCCAGCCGGC TGCGCAGGAT GAATGGCAGC 
AGCTACGTGC TCCTGAGGCA GAGCAACAAT GGAGTGCCAG CGGGGCCCTG CTCCTTCGCG 
GAGGAGCTCA GCCGCATCCT GGAGAAGAGG AAGCACACAC AGCTCGTAGA GCACCTGGAT 
GAGAGCTCTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053242.1
CDS	451..2985
Translation	MWGRLWPLLLSFLTATAVPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLVGARGALFSLNAQDIGDGTHKEIHWEASPEMQSKCHQKGKNNQTECFNHVRFLQRLNATHLYACGTHAFQPLCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHPHSLRTEEAPMHWLNDAEFVFSVLVRESQASAVGDDDKVYYFFTERAAEEGAGSFAQSRSSHRVARVARVCKGDLGGKKILQKKWTSFLKARLICHIPQYETLRSVCSLQADTSAHTHFYAAFTLTTQWKTLEASAICRYDLAEVQAVFAGPYMEYQDGVRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDFVKLHPLMARPVVPTRGRPLLLKRSVRYTHLTGTPVSTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFKDPQSVENLVISLMQHSLYVGAPSGVIQLPLSSCSRYRSCYDCILARDPYCGWDPSTHACMVTTTVANRSQNSRTALIQDMERGNRGCEGNRDTGPPPPLKTRSVLRGDDVLLPCDQPSNLARALWLLNGSMGLSDGQDGYRVGVDGLLVTDTQPEHSGNYGCYAEENGLRTLLASYSLTVRPATPAPAPQAPAMPGAQLAPDVRLLYVLAIAALGGLCLILASSLFYVACLRGSGRGHRQKYSLGRAGRAGGSAVQLQTVSGQCPGEDDDGDDGERAGGLEGGCLQIIPGEGTPAPPPPPPPPPPTELTNGLVTLPSRLRRMNGSSYVLLRQSNNGVPAGPCSFAEELSRILEKRKHTQLVEHLDESSV

Target ORF information:

RefSeq Version	XM_006053180.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G (SEMA4G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053180.1

ORF Insert Sequence:

ATGTGGGGGA GGCTCTGGCC CCTCCTCCTG AGCTTCCTCA CAGCAACTGC AGTCCCTGGA 
CCCTCACTGC GGAGACCATC CAGAGAACTA GATGCCACCC CACGGATGAC CATCCCCTAT 
GAAGAGCTCT CTGGGACCCG GCACTTCAAG GGCCAAGCCC AGAACTACTC AACACTGCTG 
CTGGAGGAGG CCTCCGCAAG GCTGCTGGTG GGAGCCCGAG GTGCCCTGTT CTCTCTCAAT 
GCGCAAGACA TAGGAGATGG GACTCACAAA GAGATTCACT GGGAGGCCTC TCCAGAGATG 
CAAAGCAAAT GTCATCAAAA AGGGAAAAAC AACCAGACCG AGTGCTTCAA CCATGTGCGA 
TTCCTGCAGC GGCTCAATGC CACCCACCTC TACGCATGTG GGACTCACGC CTTCCAGCCT 
CTCTGTGCAG CCATTGATGC TGAGGCCTTC ACCTTGCCAA CCAGCTTCGA GGAGGGGAAA 
GAGAAGTGTC CTTATGACCC AGCCCGCGGC TTCACTGGCC TCATCATTGA TGGAGGCCTC 
TACACAGCCA CTCGGTATGA ATTCCGGAGC ATCCCTGACA TCCGCCGGAG CCGGCACCCA 
CACTCCCTGA GGACAGAGGA GGCACCAATG CACTGGCTCA ATGATGCTGA GTTTGTGTTC 
TCCGTCCTGG TGCGGGAGAG CCAGGCCAGT GCCGTGGGTG ACGACGACAA AGTTTACTAC 
TTCTTCACGG AGCGTGCTGC TGAGGAGGGT GCAGGCAGCT TTGCTCAGAG CCGCAGCAGT 
CACCGCGTGG CCCGTGTGGC CCGGGTGTGC AAGGGAGACC TGGGAGGGAA GAAGATCCTG 
CAGAAGAAAT GGACCTCCTT CTTGAAGGCG CGCCTCATCT GCCACATCCC ACAGTATGAG 
ACGCTGCGCA GTGTCTGCAG CCTGCAAGCT GACACCTCAG CACACACACA CTTCTATGCG 
GCCTTCACGC TGACCACACA GTGGAAGACC CTCGAGGCCT CAGCCATATG CCGCTATGAC 
CTGGCCGAGG TGCAGGCTGT CTTCGCAGGC CCCTACATGG AATACCAGGA TGGTGTCCGG 
CGCTGGGGCC GCTACGAGGG CGGGGTGCCA GAGCCCCGGC CTGGCTCGTG CATTACAGAT 
TCACTGCGAA GCCAAGGCTA CAACTCATCC CAGGACTTGC CATCTCTGGT TCTGGACTTT 
GTGAAGTTAC ACCCACTGAT GGCTCGGCCT GTAGTGCCCA CACGTGGACG GCCCCTGCTG 
CTCAAGCGCA GTGTGCGCTA CACACACCTC ACGGGGACAC CTGTCAGCAC ACCCGCCGGA 
CCCACCTATG ACCTGCTCTT TCTGGGCACA GCTGATGGCT GGATCCACAA GGCTGTGGTC 
CTGGGCTCTG GGATGCACAT TATTGAAGAG ACACAGGTGT TCAAGGATCC CCAGTCTGTG 
GAGAACCTGG TCATCTCTCT GATGCAGCAT AGCCTCTACG TGGGGGCCCC AAGTGGCGTC 
ATCCAGCTAC CACTGTCCAG CTGCTCCCGC TACCGCTCCT GCTATGACTG CATCCTGGCC 
CGGGACCCCT ACTGTGGCTG GGACCCCAGC ACCCATGCCT GCATGGTGAC CACCACCGTA 
GCCAACAGGT CCCAAAATAG CAGGACAGCA CTCATACAGG ACATGGAGAG AGGAAACCGA 
GGCTGCGAGG GCAACAGGGA CACAGGGCCA CCACCACCAC TGAAGACCCG TTCTGTGCTC 
CGGGGTGATG ATGTCCTATT GCCCTGTGAC CAGCCATCCA ACCTGGCCCG GGCCTTGTGG 
CTGCTCAATG GGAGCATGGG CCTGAGTGAT GGGCAGGATG GCTACCGCGT GGGTGTGGAT 
GGGCTGCTGG TAACAGACAC GCAGCCTGAG CACAGCGGCA ACTACGGCTG CTATGCTGAA 
GAGAATGGCC TCCGCACCCT GCTGGCCTCC TACAGCCTCA CGGTGCGGCC AGCCACTCCA 
GCCCCTGCTC CACAAGCCCC TGCCATGCCT GGGGCACAGC TGGCACCGGA TGTGCGGCTG 
CTCTACGTGT TAGCCATTGC TGCACTTGGT GGCCTCTGCC TTATCCTGGC TTCCTCCCTC 
TTCTATGTGG CCTGTCTTCG GGGAAGTGGA CGAGGGCACC GTCAAAAATA CTCACTGGGT 
CGGGCTGGCC GGGCAGGGGG CTCTGCTGTG CAGCTGCAGA CAGTCTCAGG CCAGTGTCCT 
GGAGAGGATG ATGACGGTGA TGATGGGGAA CGGGCCGGGG GCCTAGAGGG TGGCTGCCTC 
CAGATCATCC CTGGAGAGGG AACCCCAGCC CCGCCGCCAC CGCCCCCCCC ACCGCCACCA 
ACTGAGCTGA CCAATGGGTT GGTGACACTG CCCAGCCGGC TGCGCAGGAT GAATGGCAGC 
AGCTACGTGC TCCTGAGGCA GAGCAACAAT GGAGTGCCAG CGGGGCCCTG CTCCTTCGCG 
GAGGAGCTCA GCCGCATCCT GGAGAAGAGG AAGCACACAC AGCTCGTAGA GCACCTGGAT 
GAGAGCTCTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA4G ( XM_006053180.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006053242.2 Bubalus bubalis semaphorin 4G (SEMA4G), mRNA.

Price & Availability↑

CloneID	OBu59216
Clone ID Related Accession (Same CDS sequence)	XM_006053180.2
Accession Version	XM_006053180.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	semaphorin-4G
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037567.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006053180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGGA GGCTCTGGCC CCTCCTCCTG AGCTTCCTCA CAGCAACTGC AGTCCCTGGA 
CCCTCACTGC GGAGACCATC CAGAGAACTA GATGCCACCC CACGGATGAC CATCCCCTAT 
GAAGAGCTCT CTGGGACCCG GCACTTCAAG GGCCAAGCCC AGAACTACTC AACACTGCTG 
CTGGAGGAGG CCTCCGCAAG GCTGCTGGTG GGAGCCCGAG GTGCCCTGTT CTCTCTCAAT 
GCGCAAGACA TAGGAGATGG GACTCACAAA GAGATTCACT GGGAGGCCTC TCCAGAGATG 
CAAAGCAAAT GTCATCAAAA AGGGAAAAAC AACCAGACCG AGTGCTTCAA CCATGTGCGA 
TTCCTGCAGC GGCTCAATGC CACCCACCTC TACGCATGTG GGACTCACGC CTTCCAGCCT 
CTCTGTGCAG CCATTGATGC TGAGGCCTTC ACCTTGCCAA CCAGCTTCGA GGAGGGGAAA 
GAGAAGTGTC CTTATGACCC AGCCCGCGGC TTCACTGGCC TCATCATTGA TGGAGGCCTC 
TACACAGCCA CTCGGTATGA ATTCCGGAGC ATCCCTGACA TCCGCCGGAG CCGGCACCCA 
CACTCCCTGA GGACAGAGGA GGCACCAATG CACTGGCTCA ATGATGCTGA GTTTGTGTTC 
TCCGTCCTGG TGCGGGAGAG CCAGGCCAGT GCCGTGGGTG ACGACGACAA AGTTTACTAC 
TTCTTCACGG AGCGTGCTGC TGAGGAGGGT GCAGGCAGCT TTGCTCAGAG CCGCAGCAGT 
CACCGCGTGG CCCGTGTGGC CCGGGTGTGC AAGGGAGACC TGGGAGGGAA GAAGATCCTG 
CAGAAGAAAT GGACCTCCTT CTTGAAGGCG CGCCTCATCT GCCACATCCC ACAGTATGAG 
ACGCTGCGCA GTGTCTGCAG CCTGCAAGCT GACACCTCAG CACACACACA CTTCTATGCG 
GCCTTCACGC TGACCACACA GTGGAAGACC CTCGAGGCCT CAGCCATATG CCGCTATGAC 
CTGGCCGAGG TGCAGGCTGT CTTCGCAGGC CCCTACATGG AATACCAGGA TGGTGTCCGG 
CGCTGGGGCC GCTACGAGGG CGGGGTGCCA GAGCCCCGGC CTGGCTCGTG CATTACAGAT 
TCACTGCGAA GCCAAGGCTA CAACTCATCC CAGGACTTGC CATCTCTGGT TCTGGACTTT 
GTGAAGTTAC ACCCACTGAT GGCTCGGCCT GTAGTGCCCA CACGTGGACG GCCCCTGCTG 
CTCAAGCGCA GTGTGCGCTA CACACACCTC ACGGGGACAC CTGTCAGCAC ACCCGCCGGA 
CCCACCTATG ACCTGCTCTT TCTGGGCACA GCTGATGGCT GGATCCACAA GGCTGTGGTC 
CTGGGCTCTG GGATGCACAT TATTGAAGAG ACACAGGTGT TCAAGGATCC CCAGTCTGTG 
GAGAACCTGG TCATCTCTCT GATGCAGCAT AGCCTCTACG TGGGGGCCCC AAGTGGCGTC 
ATCCAGCTAC CACTGTCCAG CTGCTCCCGC TACCGCTCCT GCTATGACTG CATCCTGGCC 
CGGGACCCCT ACTGTGGCTG GGACCCCAGC ACCCATGCCT GCATGGTGAC CACCACCGTA 
GCCAACAGGT CCCAAAATAG CAGGACAGCA CTCATACAGG ACATGGAGAG AGGAAACCGA 
GGCTGCGAGG GCAACAGGGA CACAGGGCCA CCACCACCAC TGAAGACCCG TTCTGTGCTC 
CGGGGTGATG ATGTCCTATT GCCCTGTGAC CAGCCATCCA ACCTGGCCCG GGCCTTGTGG 
CTGCTCAATG GGAGCATGGG CCTGAGTGAT GGGCAGGATG GCTACCGCGT GGGTGTGGAT 
GGGCTGCTGG TAACAGACAC GCAGCCTGAG CACAGCGGCA ACTACGGCTG CTATGCTGAA 
GAGAATGGCC TCCGCACCCT GCTGGCCTCC TACAGCCTCA CGGTGCGGCC AGCCACTCCA 
GCCCCTGCTC CACAAGCCCC TGCCATGCCT GGGGCACAGC TGGCACCGGA TGTGCGGCTG 
CTCTACGTGT TAGCCATTGC TGCACTTGGT GGCCTCTGCC TTATCCTGGC TTCCTCCCTC 
TTCTATGTGG CCTGTCTTCG GGGAAGTGGA CGAGGGCACC GTCAAAAATA CTCACTGGGT 
CGGGCTGGCC GGGCAGGGGG CTCTGCTGTG CAGCTGCAGA CAGTCTCAGG CCAGTGTCCT 
GGAGAGGATG ATGACGGTGA TGATGGGGAA CGGGCCGGGG GCCTAGAGGG TGGCTGCCTC 
CAGATCATCC CTGGAGAGGG AACCCCAGCC CCGCCGCCAC CGCCCCCCCC ACCGCCACCA 
ACTGAGCTGA CCAATGGGTT GGTGACACTG CCCAGCCGGC TGCGCAGGAT GAATGGCAAC 
AGCTACGTGC TCCTGAGGCA GAGCAACAAT GGAGTGCCAG CGGGGCCCTG CTCCTTCGCG 
GAGGAGCTCA GCCGCATCCT GGAGAAGAGG AAGCACACAC AGCTCGTAGA GCACCTGGAT 
GAGAGCTCTG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006053242.2
CDS	476..3010
Translation	MWGRLWPLLLSFLTATAVPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLVGARGALFSLNAQDIGDGTHKEIHWEASPEMQSKCHQKGKNNQTECFNHVRFLQRLNATHLYACGTHAFQPLCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHPHSLRTEEAPMHWLNDAEFVFSVLVRESQASAVGDDDKVYYFFTERAAEEGAGSFAQSRSSHRVARVARVCKGDLGGKKILQKKWTSFLKARLICHIPQYETLRSVCSLQADTSAHTHFYAAFTLTTQWKTLEASAICRYDLAEVQAVFAGPYMEYQDGVRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDFVKLHPLMARPVVPTRGRPLLLKRSVRYTHLTGTPVSTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFKDPQSVENLVISLMQHSLYVGAPSGVIQLPLSSCSRYRSCYDCILARDPYCGWDPSTHACMVTTTVANRSQNSRTALIQDMERGNRGCEGNRDTGPPPPLKTRSVLRGDDVLLPCDQPSNLARALWLLNGSMGLSDGQDGYRVGVDGLLVTDTQPEHSGNYGCYAEENGLRTLLASYSLTVRPATPAPAPQAPAMPGAQLAPDVRLLYVLAIAALGGLCLILASSLFYVACLRGSGRGHRQKYSLGRAGRAGGSAVQLQTVSGQCPGEDDDGDDGERAGGLEGGCLQIIPGEGTPAPPPPPPPPPPTELTNGLVTLPSRLRRMNGNSYVLLRQSNNGVPAGPCSFAEELSRILEKRKHTQLVEHLDESSV

Target ORF information:

RefSeq Version	XM_006053180.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis semaphorin 4G (SEMA4G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006053180.2

ORF Insert Sequence:

ATGTGGGGGA GGCTCTGGCC CCTCCTCCTG AGCTTCCTCA CAGCAACTGC AGTCCCTGGA 
CCCTCACTGC GGAGACCATC CAGAGAACTA GATGCCACCC CACGGATGAC CATCCCCTAT 
GAAGAGCTCT CTGGGACCCG GCACTTCAAG GGCCAAGCCC AGAACTACTC AACACTGCTG 
CTGGAGGAGG CCTCCGCAAG GCTGCTGGTG GGAGCCCGAG GTGCCCTGTT CTCTCTCAAT 
GCGCAAGACA TAGGAGATGG GACTCACAAA GAGATTCACT GGGAGGCCTC TCCAGAGATG 
CAAAGCAAAT GTCATCAAAA AGGGAAAAAC AACCAGACCG AGTGCTTCAA CCATGTGCGA 
TTCCTGCAGC GGCTCAATGC CACCCACCTC TACGCATGTG GGACTCACGC CTTCCAGCCT 
CTCTGTGCAG CCATTGATGC TGAGGCCTTC ACCTTGCCAA CCAGCTTCGA GGAGGGGAAA 
GAGAAGTGTC CTTATGACCC AGCCCGCGGC TTCACTGGCC TCATCATTGA TGGAGGCCTC 
TACACAGCCA CTCGGTATGA ATTCCGGAGC ATCCCTGACA TCCGCCGGAG CCGGCACCCA 
CACTCCCTGA GGACAGAGGA GGCACCAATG CACTGGCTCA ATGATGCTGA GTTTGTGTTC 
TCCGTCCTGG TGCGGGAGAG CCAGGCCAGT GCCGTGGGTG ACGACGACAA AGTTTACTAC 
TTCTTCACGG AGCGTGCTGC TGAGGAGGGT GCAGGCAGCT TTGCTCAGAG CCGCAGCAGT 
CACCGCGTGG CCCGTGTGGC CCGGGTGTGC AAGGGAGACC TGGGAGGGAA GAAGATCCTG 
CAGAAGAAAT GGACCTCCTT CTTGAAGGCG CGCCTCATCT GCCACATCCC ACAGTATGAG 
ACGCTGCGCA GTGTCTGCAG CCTGCAAGCT GACACCTCAG CACACACACA CTTCTATGCG 
GCCTTCACGC TGACCACACA GTGGAAGACC CTCGAGGCCT CAGCCATATG CCGCTATGAC 
CTGGCCGAGG TGCAGGCTGT CTTCGCAGGC CCCTACATGG AATACCAGGA TGGTGTCCGG 
CGCTGGGGCC GCTACGAGGG CGGGGTGCCA GAGCCCCGGC CTGGCTCGTG CATTACAGAT 
TCACTGCGAA GCCAAGGCTA CAACTCATCC CAGGACTTGC CATCTCTGGT TCTGGACTTT 
GTGAAGTTAC ACCCACTGAT GGCTCGGCCT GTAGTGCCCA CACGTGGACG GCCCCTGCTG 
CTCAAGCGCA GTGTGCGCTA CACACACCTC ACGGGGACAC CTGTCAGCAC ACCCGCCGGA 
CCCACCTATG ACCTGCTCTT TCTGGGCACA GCTGATGGCT GGATCCACAA GGCTGTGGTC 
CTGGGCTCTG GGATGCACAT TATTGAAGAG ACACAGGTGT TCAAGGATCC CCAGTCTGTG 
GAGAACCTGG TCATCTCTCT GATGCAGCAT AGCCTCTACG TGGGGGCCCC AAGTGGCGTC 
ATCCAGCTAC CACTGTCCAG CTGCTCCCGC TACCGCTCCT GCTATGACTG CATCCTGGCC 
CGGGACCCCT ACTGTGGCTG GGACCCCAGC ACCCATGCCT GCATGGTGAC CACCACCGTA 
GCCAACAGGT CCCAAAATAG CAGGACAGCA CTCATACAGG ACATGGAGAG AGGAAACCGA 
GGCTGCGAGG GCAACAGGGA CACAGGGCCA CCACCACCAC TGAAGACCCG TTCTGTGCTC 
CGGGGTGATG ATGTCCTATT GCCCTGTGAC CAGCCATCCA ACCTGGCCCG GGCCTTGTGG 
CTGCTCAATG GGAGCATGGG CCTGAGTGAT GGGCAGGATG GCTACCGCGT GGGTGTGGAT 
GGGCTGCTGG TAACAGACAC GCAGCCTGAG CACAGCGGCA ACTACGGCTG CTATGCTGAA 
GAGAATGGCC TCCGCACCCT GCTGGCCTCC TACAGCCTCA CGGTGCGGCC AGCCACTCCA 
GCCCCTGCTC CACAAGCCCC TGCCATGCCT GGGGCACAGC TGGCACCGGA TGTGCGGCTG 
CTCTACGTGT TAGCCATTGC TGCACTTGGT GGCCTCTGCC TTATCCTGGC TTCCTCCCTC 
TTCTATGTGG CCTGTCTTCG GGGAAGTGGA CGAGGGCACC GTCAAAAATA CTCACTGGGT 
CGGGCTGGCC GGGCAGGGGG CTCTGCTGTG CAGCTGCAGA CAGTCTCAGG CCAGTGTCCT 
GGAGAGGATG ATGACGGTGA TGATGGGGAA CGGGCCGGGG GCCTAGAGGG TGGCTGCCTC 
CAGATCATCC CTGGAGAGGG AACCCCAGCC CCGCCGCCAC CGCCCCCCCC ACCGCCACCA 
ACTGAGCTGA CCAATGGGTT GGTGACACTG CCCAGCCGGC TGCGCAGGAT GAATGGCAAC 
AGCTACGTGC TCCTGAGGCA GAGCAACAAT GGAGTGCCAG CGGGGCCCTG CTCCTTCGCG 
GAGGAGCTCA GCCGCATCCT GGAGAAGAGG AAGCACACAC AGCTCGTAGA GCACCTGGAT 
GAGAGCTCTG TCTGA