ORF » Species Summary » Bubalus bubalis » MAP4K4 cDNA ORF clone

MAP4K4 cDNA ORF clone, Bubalus bubalis(water buffalo)

The following MAP4K4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP4K4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBu11556 XM_006054059.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBu45780 XM_025260724.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45781 XM_025260725.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45782 XM_025260726.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45783 XM_025260727.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45784 XM_025260728.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45785 XM_025260729.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45786 XM_025260731.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45787 XM_025260732.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45788 XM_025260733.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBu45789 XM_025260734.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45790 XM_025260735.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45791 XM_025260736.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45792 XM_025260737.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45793 XM_025260738.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45794 XM_025260739.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45795 XM_025260740.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45796 XM_025260742.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45797 XM_025260743.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45798 XM_025260744.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45799 XM_025260745.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45800 XM_025260746.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45801 XM_025260747.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45802 XM_025260748.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45803 XM_025260749.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45804 XM_025260750.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45805 XM_025260751.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45806 XM_025260752.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45807 XM_025260753.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45808 XM_025260754.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45809 XM_025260755.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBu45810 XM_025260756.1
Latest version!		 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    MAP4K4 ( XM_006054059.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006054121.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.
    Price & Availability↑
    CloneID OBu11556
    Clone ID Related Accession (Same CDS sequence) XM_006054059.1
    Accession Version XM_006054059.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2014-03-11
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784582.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACAGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTGC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ATAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC ATACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCGGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGCG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCCGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGAGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAATACATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGCAC 
    GACCCCCGGA GGCCGCCCCC GCCACCAGAC AGGAGCAAGG CTGGCCTGCA CGAGCCCAAG 
    GCCCCGCACG AGCCCGCTGA CCGGGCCCGC GAGGTGGAAG ATAGATTTAG GAAAACTAAC 
    CACAGCTCCC CCGAAGCCCA GTCTAAGCAG ACGGGCCGAG TATTGGAACC TCCAGTGCCT 
    TCCAGATCAG AGTCTTTTTC CAGTGGCAGC TCCGAGTCTG TGCACCCTGC CCTGCAGAGA 
    CCCGCCGAGC CACAGGTCCC TGTGAGAACA ACGTCACGGT CCCCCGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AGTAACCAAG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCAAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGACCTGAC GGCGCTGGCC AAAGAGCTGC GGGCCGTGGA GGACGTGCGG 
    CCGCCGCACA AGGTGACGGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC GACGGACGAG 
    GAGGACGACG ACGCGGAGCA GGAAGGGGCC GAGGAGCCCA CGTCTGGCCC GGAGGACACC 
    AGAGCCGCAT CATCTCTGAA CTTGAGCAAC GGTGAAACGG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACAT CCAATGCAGC 
    ATCAAACCCC ACGCGATCAT CATCCTCCCC AACACAGACG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTAAACACA TATGGGAGGA TCACCAAGGA CGTGGTTCTG 
    CAGTGGGGAG AGATGCCGAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CACAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
    TCGGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006054121.1
    CDS119..3550
    Translation

    Target ORF information:

    RefSeq Version XM_006054059.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_006054059.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACAGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTGC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ATAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC ATACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCGGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGCG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCCGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGAGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAATACATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGCAC 
    GACCCCCGGA GGCCGCCCCC GCCACCAGAC AGGAGCAAGG CTGGCCTGCA CGAGCCCAAG 
    GCCCCGCACG AGCCCGCTGA CCGGGCCCGC GAGGTGGAAG ATAGATTTAG GAAAACTAAC 
    CACAGCTCCC CCGAAGCCCA GTCTAAGCAG ACGGGCCGAG TATTGGAACC TCCAGTGCCT 
    TCCAGATCAG AGTCTTTTTC CAGTGGCAGC TCCGAGTCTG TGCACCCTGC CCTGCAGAGA 
    CCCGCCGAGC CACAGGTCCC TGTGAGAACA ACGTCACGGT CCCCCGTCCT GTCCCGCCGG 
    GATTCCCCAC TGCAGGGCAG TGGGCAGCAG AGTAACCAAG CTGGTCAGAG AAACTCCACC 
    AGTAGCATCG AGCCAAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGACCTGAC GGCGCTGGCC AAAGAGCTGC GGGCCGTGGA GGACGTGCGG 
    CCGCCGCACA AGGTGACGGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC GACGGACGAG 
    GAGGACGACG ACGCGGAGCA GGAAGGGGCC GAGGAGCCCA CGTCTGGCCC GGAGGACACC 
    AGAGCCGCAT CATCTCTGAA CTTGAGCAAC GGTGAAACGG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACAT CCAATGCAGC 
    ATCAAACCCC ACGCGATCAT CATCCTCCCC AACACAGACG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTAAACACA TATGGGAGGA TCACCAAGGA CGTGGTTCTG 
    CAGTGGGGAG AGATGCCGAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CACAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
    TCGGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260724.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116509.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBu45780
    Clone ID Related Accession (Same CDS sequence) XM_025260724.1
    Accession Version XM_025260724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116509.1
    CDS125..4273
    Translation

    Target ORF information:

    RefSeq Version XM_025260724.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260725.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116510.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBu45781
    Clone ID Related Accession (Same CDS sequence) XM_025260725.1
    Accession Version XM_025260725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGGGC CTGAAGAGTT ATCTGTCCTC TTAAGCTCTC CTCTTTCTGC ATGGATTCTC 
    AGAAATTATA ACTTGGCTTC AGGATTGTGT GGTCACGGCC ACCTGGCTGC TGAGTCTCTG 
    CTGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116510.1
    CDS128..4276
    Translation

    Target ORF information:

    RefSeq Version XM_025260725.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGAAGGGC CTGAAGAGTT ATCTGTCCTC TTAAGCTCTC CTCTTTCTGC ATGGATTCTC 
    AGAAATTATA ACTTGGCTTC AGGATTGTGT GGTCACGGCC ACCTGGCTGC TGAGTCTCTG 
    CTGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CGTAAGAAAG AACCCACACT CTATGATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260726.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116511.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBu45782
    Clone ID Related Accession (Same CDS sequence) XM_025260726.1
    Accession Version XM_025260726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGCATCGA GCCAAGGCTG CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC 
    AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCAG GGTCCCAGCC CGGCTCCCAC 
    CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA 
    GGCTCGCCAT CTCAGCGCCT TGAAAACGCA GCAAAAAAGC CTGAAGATAA AAAGGAAGTG 
    TTTAGACCTC TCAAGCCTGC TGGTGAAGTG GACCTGACGG CGCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ACGTGCGGCC GCCGCACAAG GTGACGGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACGA CGGACGAGGA GGACGACGAC GCGGAGCAGG AAGGGGCCGA GGAGCCCACG 
    TCTGGCCCGG AGGACACCAG AGCCGCATCA TCTCTGAACT TGAGCAACGG TGAAACGGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCCGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACTAACACTC GGCCCCAGAG CGACACGCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCTGAGATCC TGTGCGCTGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC TGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTGG TGACGATATC TGGGAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCA 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCGT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC GTTTGGCGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCGGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG CAGCATCAAA 
    CCCCACGCGA TCATCATCCT CCCCAACACA GACGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTAAA CACGTATGGG AGGATCACCA AGGATGTGGT TCTGCAGTGG 
    GGAGAGATGC CGACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCACAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCGGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116511.1
    CDS125..4270
    Translation

    Target ORF information:

    RefSeq Version XM_025260726.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGCATCGA GCCAAGGCTG CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC 
    AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC AGCAACTCAG GGTCCCAGCC CGGCTCCCAC 
    CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC TTCAGAGTGA GATCATCATC CAAATCTGAA 
    GGCTCGCCAT CTCAGCGCCT TGAAAACGCA GCAAAAAAGC CTGAAGATAA AAAGGAAGTG 
    TTTAGACCTC TCAAGCCTGC TGGTGAAGTG GACCTGACGG CGCTGGCCAA AGAGCTGCGG 
    GCCGTGGAGG ACGTGCGGCC GCCGCACAAG GTGACGGACT ACTCCTCCTC CAGCGAGGAG 
    TCGGGGACGA CGGACGAGGA GGACGACGAC GCGGAGCAGG AAGGGGCCGA GGAGCCCACG 
    TCTGGCCCGG AGGACACCAG AGCCGCATCA TCTCTGAACT TGAGCAACGG TGAAACGGAG 
    TCCGTGAAAA CCATGGTTGT CCATGACGAC GTGGAGAGTG AACCAGCCCT GACCCCGTCC 
    AAAGAGGGCA CGCTGATCGT CCGCCAGAGT GCAGTTGACC AAAAGCGCGC CAGCCATCAT 
    GAGAGCAATG GCTTTGCGGG TCGCATTCAC CTCTTGCCAG ATCTCTTACA GCAAAGCCAT 
    TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC CCATCCTCCA GCCAGCCGAC ACCCACCATG 
    TCCCCACAGA CACCCCAGGA CAAGCTCACT ACTAATGAGA CTCAGTCCGC TAGTAACACA 
    CTCCAGAAAC ACAAATCTTC CTCCTCCTTT ACACCTTTTA TAGACCCCAG ATTACTACAG 
    ATTTCTCCAT CTAGCGGGAC AACAGTGACT TCTGTGGTGG GGTTTTCCTG TGATGGAATG 
    AGACCCGAGG CCATGAGGCA AGATCCTACG CGGAAGGGCT CAGTGGTCAA TGTCAATCCC 
    ACTAACACTC GGCCCCAGAG CGACACGCCA GAGATTCGCA AATACAAGAA GAGGTTTAAC 
    TCTGAGATCC TGTGCGCTGC CTTGTGGGGA GTGAACTTGT TGGTGGGTAC TGAGAGTGGC 
    CTGATGCTGC TGGACAGAAG TGGCCAAGGG AAGGTGTATC CTCTGATCAA CAGAAGGCGA 
    TTTCAGCAGA TGGACGTCCT GGAAGGCTTG AACGTCTTGG TGACGATATC TGGGAAAAAG 
    GATAAGTTAC GTGTCTACTA TTTGTCCTGG TTAAGAAATA AAATACTTCA CAACGACCCA 
    GAGGTTGAGA AGAAGCAGGG CTGGACGACA GTGGGGGACC TGGAAGGCTG TGTGCATTAT 
    AAAGTTGTAA AATATGAAAG GATCAAATTT CTGGTCATTG CTTTGAAGAA CTCTGTGGAA 
    GTCTACGCGT GGGCACCCAA GCCATATCAC AAATTCATGG CCTTTAAGTC GTTTGGCGAG 
    CTGGTGCACA AGCCGTTGCT GGTGGATCTC ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG 
    ATCTATGGAT CCTGTGCTGG ATTCCATGCT GTTGATGTGG ACTCGGGATC GGTCTATGAC 
    ATTTACCTAC CAACACACGT AAGAAAGAAC CCACACTCTA TGATCCAATG CAGCATCAAA 
    CCCCACGCGA TCATCATCCT CCCCAACACA GACGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTAAA CACGTATGGG AGGATCACCA AGGATGTGGT TCTGCAGTGG 
    GGAGAGATGC CGACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCACAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCGGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260727.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116512.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBu45783
    Clone ID Related Accession (Same CDS sequence) XM_025260727.1
    Accession Version XM_025260727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
    AATGTTTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCTCC CAAATTTGCA 
    CACCACCATC TTCGCTCTCA GGACCCCTGC CCACCTTCCC GCAGTGAGGT GCTCAGTCAG 
    AGTTCTGACT CTAAGGCGGA GGTGCCCGAC CCAGCCCAGA AGGCTTGGTC TAGATCAGAC 
    AGTGACGAGG TGCCTCCAAG GGTCCCTGTG AGAACAACGT CACGGTCCCC CGTCCTGTCC 
    CGCCGGGATT CCCCACTGCA GGGCAGTGGG CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC 
    TCCACCAGTA GCATCGAGCC AAGGCTGCTC TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG 
    CCAGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCAGGGT CCCAGCCCGG CTCCCACCCA 
    GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC AGAGTGAGAT CATCATCCAA ATCTGAAGGC 
    TCGCCATCTC AGCGCCTTGA AAACGCAGCA AAAAAGCCTG AAGATAAAAA GGAAGTGTTT 
    AGACCTCTCA AGCCTGCTGG TGAAGTGGAC CTGACGGCGC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGACG TGCGGCCGCC GCACAAGGTG ACGGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACGACGG ACGAGGAGGA CGACGACGCG GAGCAGGAAG GGGCCGAGGA GCCCACGTCT 
    GGCCCGGAGG ACACCAGAGC CGCATCATCT CTGAACTTGA GCAACGGTGA AACGGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGAGTGCA GTTGACCAAA AGCGCGCCAG CCATCATGAG 
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
    TCCTCCACTT CCTCCACCTC CTCTTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
    CCACAGACAC CCCAGGACAA GCTCACTACT AATGAGACTC AGTCCGCTAG TAACACACTC 
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
    TCTCCATCTA GCGGGACAAC AGTGACTTCT GTGGTGGGGT TTTCCTGTGA TGGAATGAGA 
    CCCGAGGCCA TGAGGCAAGA TCCTACGCGG AAGGGCTCAG TGGTCAATGT CAATCCCACT 
    AACACTCGGC CCCAGAGCGA CACGCCAGAG ATTCGCAAAT ACAAGAAGAG GTTTAACTCT 
    GAGATCCTGT GCGCTGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACTGA GAGTGGCCTG 
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGGCGATTT 
    CAGCAGATGG ACGTCCTGGA AGGCTTGAAC GTCTTGGTGA CGATATCTGG GAAAAAGGAT 
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGACCCAGAG 
    GTTGAGAAGA AGCAGGGCTG GACGACAGTG GGGGACCTGG AAGGCTGTGT GCATTATAAA 
    GTTGTAAAAT ATGAAAGGAT CAAATTTCTG GTCATTGCTT TGAAGAACTC TGTGGAAGTC 
    TACGCGTGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCGTT TGGCGAGCTG 
    GTGCACAAGC CGTTGCTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGCT GAAAGTGATC 
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGACT CGGGATCGGT CTATGACATT 
    TACCTACCAA CACACGTAAG AAAGAACCCA CACTCTATGA TCCAATGCAG CATCAAACCC 
    CACGCGATCA TCATCCTCCC CAACACAGAC GGCATGGAGC TCCTGGTGTG CTATGAAGAT 
    GAAGGGGTTT ACGTAAACAC GTATGGGAGG ATCACCAAGG ATGTGGTTCT GCAGTGGGGA 
    GAGATGCCGA CGTCTGTAGC ATATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
    GCCATAGAGA TCCGATCTGT GGAAACTGGC CACTTGGATG GCGTGTTTAT GCACAAAAGG 
    GCTCAAAGGC TGAAGTTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCGGTCCGG 
    TCTGGGGGCA GCAGCCAGGT TTACTTCATG ACTCTCGGCA GGACCTCTCT TCTGAGCTGG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116512.1
    CDS125..4267
    Translation

    Target ORF information:

    RefSeq Version XM_025260727.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGTGGTCCC ACCTGGCATC TCTCAAGAAC 
    AATGTTTCCC CTGTCTCGAG ATCCCATTCC TTCAGTGACC CTTCTCCTCC CAAATTTGCA 
    CACCACCATC TTCGCTCTCA GGACCCCTGC CCACCTTCCC GCAGTGAGGT GCTCAGTCAG 
    AGTTCTGACT CTAAGGCGGA GGTGCCCGAC CCAGCCCAGA AGGCTTGGTC TAGATCAGAC 
    AGTGACGAGG TGCCTCCAAG GGTCCCTGTG AGAACAACGT CACGGTCCCC CGTCCTGTCC 
    CGCCGGGATT CCCCACTGCA GGGCAGTGGG CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC 
    TCCACCAGTA GCATCGAGCC AAGGCTGCTC TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG 
    CCAGGCAGTG GCAGCTCCTC GGGGTCCAGC AACTCAGGGT CCCAGCCCGG CTCCCACCCA 
    GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC AGAGTGAGAT CATCATCCAA ATCTGAAGGC 
    TCGCCATCTC AGCGCCTTGA AAACGCAGCA AAAAAGCCTG AAGATAAAAA GGAAGTGTTT 
    AGACCTCTCA AGCCTGCTGG TGAAGTGGAC CTGACGGCGC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGACG TGCGGCCGCC GCACAAGGTG ACGGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACGACGG ACGAGGAGGA CGACGACGCG GAGCAGGAAG GGGCCGAGGA GCCCACGTCT 
    GGCCCGGAGG ACACCAGAGC CGCATCATCT CTGAACTTGA GCAACGGTGA AACGGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGAGTGCA GTTGACCAAA AGCGCGCCAG CCATCATGAG 
    AGCAATGGCT TTGCGGGTCG CATTCACCTC TTGCCAGATC TCTTACAGCA AAGCCATTCC 
    TCCTCCACTT CCTCCACCTC CTCTTCCCCA TCCTCCAGCC AGCCGACACC CACCATGTCC 
    CCACAGACAC CCCAGGACAA GCTCACTACT AATGAGACTC AGTCCGCTAG TAACACACTC 
    CAGAAACACA AATCTTCCTC CTCCTTTACA CCTTTTATAG ACCCCAGATT ACTACAGATT 
    TCTCCATCTA GCGGGACAAC AGTGACTTCT GTGGTGGGGT TTTCCTGTGA TGGAATGAGA 
    CCCGAGGCCA TGAGGCAAGA TCCTACGCGG AAGGGCTCAG TGGTCAATGT CAATCCCACT 
    AACACTCGGC CCCAGAGCGA CACGCCAGAG ATTCGCAAAT ACAAGAAGAG GTTTAACTCT 
    GAGATCCTGT GCGCTGCCTT GTGGGGAGTG AACTTGTTGG TGGGTACTGA GAGTGGCCTG 
    ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG GTGTATCCTC TGATCAACAG AAGGCGATTT 
    CAGCAGATGG ACGTCCTGGA AGGCTTGAAC GTCTTGGTGA CGATATCTGG GAAAAAGGAT 
    AAGTTACGTG TCTACTATTT GTCCTGGTTA AGAAATAAAA TACTTCACAA CGACCCAGAG 
    GTTGAGAAGA AGCAGGGCTG GACGACAGTG GGGGACCTGG AAGGCTGTGT GCATTATAAA 
    GTTGTAAAAT ATGAAAGGAT CAAATTTCTG GTCATTGCTT TGAAGAACTC TGTGGAAGTC 
    TACGCGTGGG CACCCAAGCC ATATCACAAA TTCATGGCCT TTAAGTCGTT TGGCGAGCTG 
    GTGCACAAGC CGTTGCTGGT GGATCTCACT GTTGAGGAAG GCCAGAGGCT GAAAGTGATC 
    TATGGATCCT GTGCTGGATT CCATGCTGTT GATGTGGACT CGGGATCGGT CTATGACATT 
    TACCTACCAA CACACGTAAG AAAGAACCCA CACTCTATGA TCCAATGCAG CATCAAACCC 
    CACGCGATCA TCATCCTCCC CAACACAGAC GGCATGGAGC TCCTGGTGTG CTATGAAGAT 
    GAAGGGGTTT ACGTAAACAC GTATGGGAGG ATCACCAAGG ATGTGGTTCT GCAGTGGGGA 
    GAGATGCCGA CGTCTGTAGC ATATATTCGA TCCAATCAGA CGATGGGCTG GGGAGAAAAG 
    GCCATAGAGA TCCGATCTGT GGAAACTGGC CACTTGGATG GCGTGTTTAT GCACAAAAGG 
    GCTCAAAGGC TGAAGTTCTT GTGTGAACGC AATGACAAGG TGTTCTTTGC CTCGGTCCGG 
    TCTGGGGGCA GCAGCCAGGT TTACTTCATG ACTCTCGGCA GGACCTCTCT TCTGAGCTGG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260728.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116513.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OBu45784
    Clone ID Related Accession (Same CDS sequence) XM_025260728.1
    Accession Version XM_025260728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4140bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116513.1
    CDS125..4264
    Translation

    Target ORF information:

    RefSeq Version XM_025260728.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260729.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116514.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBu45785
    Clone ID Related Accession (Same CDS sequence) XM_025260729.1
    Accession Version XM_025260729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CATCCAATGC AGCATCAAAC CCCACGCGAT CATCATCCTC 
    CCCAACACAG ACGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTAAAC 
    ACGTATGGGA GGATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC GACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCACAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116514.1
    CDS124..4248
    Translation

    Target ORF information:

    RefSeq Version XM_025260729.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CATCCAATGC AGCATCAAAC CCCACGCGAT CATCATCCTC 
    CCCAACACAG ACGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTAAAC 
    ACGTATGGGA GGATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC GACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCACAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260731.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116516.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBu45786
    Clone ID Related Accession (Same CDS sequence) XM_025260731.1
    Accession Version XM_025260731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGGCAG CGCTGTCTAC CAGGGAAGCT CCTTGGATAC CCAGTGCCTG GGGTTTTATT 
    TGGAGATGGT CACATAAGCA CTGTCTGCCT GGCATGTCCC CCAACTTTCA CTCTCCCCGC 
    TGGGAGGATG AAGAGGAGGA AATCAAACTG GAGATAAATA TGCTGAAGAA ATATTCTCAT 
    CATAGAAATA TTGCAACGTA TTACGGTGCT TTCATTAAAA AGAGCCCCCC AGGACACGAT 
    GACCAGCTTT GGCTCGTTAT GGAGTTCTGT GGGGCCGGAT CCATCACAGA CCTGGTGAAG 
    AACACCAAGG GCAACACGCT AAAGGAAGAC TGGATAGCCT ACATCTCCAG GGAGATCCTG 
    CGGGGCCTGG CCCATCTCCA CATCCATCAC GTGATCCACC GCGACATCAA GGGCCAGAAT 
    GTGCTGCTGA CCGAGAACGC AGAGGTGAAG CTGGTGGACT TCGGGGTGAG TGCCCAGCTG 
    GACAGGACCG TGGGGAGGAG GAACACGTTC ATAGGCACCC CATACTGGAT GGCCCCTGAG 
    GTCATTGCCT GTGACGAGAA CCCGGACGCC ACCTACGATT ACAGGAGTGA CCTCTGGTCC 
    TGTGGCATCA CTGCCATTGA GATGGCTGAA GGCGCTCCCC CTCTCTGCGA CATGCATCCC 
    ATGAGAGCCC TGTTTCTCAT CCCCCGGAAT CCTCCTCCCC GGCTGAAGTC CAAAAAATGG 
    TCCAAGAAGT TTTTTAGTTT TATAGAAGGA TGCCTGGTGA AGAATTACAT GCAGCGGCCC 
    TCCACCGAGC AGCTTCTGAA GCATCCTTTC ATTAGGGATC AGCCGAACGA AAGGCAGGTT 
    AGAATCCAGC TTAAGGACCA TATAGACCGG ACCAGGAAGA AGAGAGGAGA GAAAGATGAA 
    ACCGAGTATG AGTACAGTGG GAGTGAGGAA GAGGAGGAGG AGGCGCCTGA GCAGGAGGGC 
    GAGCCCAGCT CCATCGTCAA CGTGCCGGGG GAGTCGACGC TGCGCCGGGA CTTCCTGCGG 
    CTCCAGCAGG AGAACAAGGA GCGCTCCGAG GCCCTGAGGA GGCAGCAGCT CCTGCAGGAG 
    CAGCAGCTCC GCGAGCAGGA GGAGTATAAA CGGCAGCTGC TGGCGGAGAG GCAGAAGCGG 
    ATCGAGCAGC AGAAGGAGCA GAGGCGGCGG CTGGAAGAGC AACAGAGGAG AGAGCGGGAA 
    GCCAGAAGGC AGCAGGAACG TGAGCAGCGG AGGAGGGAAC AGGAGGAGAA GAGGCGGCTG 
    GAGGAGCTGG AGAGACGACG GAAGGAGGAG GAGGAGCGGA GGCGGGCGGA GGAGGAGAAG 
    AGGAGAGTGG AGAGGGAGCA GGAATACATC CGACGGCAGC TGGAAGAGGA ACAGCGTCAC 
    CTGGAACTGC TTCAGCAGCA GCTGCTCCAG GAGCAGGCCA TGCTACTGGA GTGCCGCTGG 
    CGGGAGCTGG AGGAGCACCG GCAGGCAGAG CGGCTCCAGA GGCAGCTGCA GCAAGAACAA 
    GCCTATCTCC TGTCTCTACA GCACGACCCC CGGAGGCCGC CCCCGCCGCC AGACAGGAGC 
    AAGGCTGGCC TGCACGAGCC CAAGGCCCCG CACGAGCCCG CTGACCGGGC CCGCGAGGTG 
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCCGAAG CCCAGTCTAA GCAGACGGGC 
    CGAGTATTGG AACCTCCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAGTGG CAGCTCCGAG 
    TCTGTGCACC CTGCCCTGCA GAGACCCGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG AGATCCCATT CCTTCAGTGA CCCTTCTCCT 
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCCT GCCCACCTTC CCGCAGTGAG 
    GTGCTCAGTC AGAGTTCTGA CTCTAAGGCG GAGGTGCCCG ACCCAGCCCA GAAGGCTTGG 
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCAAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCAGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC GCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA CGTGCGGCCG CCGCACAAGG TGACGGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACGAC GGACGAGGAG GACGACGACG CGGAGCAGGA AGGGGCCGAG 
    GAGCCCACGT CTGGCCCGGA GGACACCAGA GCCGCATCAT CTCTGAACTT GAGCAACGGT 
    GAAACGGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCCGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CTAACACTCG GCCCCAGAGC GACACGCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CTGAGATCCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACT 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTGGT GACGATATCT 
    GGGAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCAG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
    TTTGGCGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCGGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGC 
    AGCATCAAAC CCCACGCGAT CATCATCCTC CCCAACACAG ACGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTAAAC ACGTATGGGA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAGATGCC GACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCACAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
    GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116516.1
    CDS175..4269
    Translation

    Target ORF information:

    RefSeq Version XM_025260731.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCGGGCAG CGCTGTCTAC CAGGGAAGCT CCTTGGATAC CCAGTGCCTG GGGTTTTATT 
    TGGAGATGGT CACATAAGCA CTGTCTGCCT GGCATGTCCC CCAACTTTCA CTCTCCCCGC 
    TGGGAGGATG AAGAGGAGGA AATCAAACTG GAGATAAATA TGCTGAAGAA ATATTCTCAT 
    CATAGAAATA TTGCAACGTA TTACGGTGCT TTCATTAAAA AGAGCCCCCC AGGACACGAT 
    GACCAGCTTT GGCTCGTTAT GGAGTTCTGT GGGGCCGGAT CCATCACAGA CCTGGTGAAG 
    AACACCAAGG GCAACACGCT AAAGGAAGAC TGGATAGCCT ACATCTCCAG GGAGATCCTG 
    CGGGGCCTGG CCCATCTCCA CATCCATCAC GTGATCCACC GCGACATCAA GGGCCAGAAT 
    GTGCTGCTGA CCGAGAACGC AGAGGTGAAG CTGGTGGACT TCGGGGTGAG TGCCCAGCTG 
    GACAGGACCG TGGGGAGGAG GAACACGTTC ATAGGCACCC CATACTGGAT GGCCCCTGAG 
    GTCATTGCCT GTGACGAGAA CCCGGACGCC ACCTACGATT ACAGGAGTGA CCTCTGGTCC 
    TGTGGCATCA CTGCCATTGA GATGGCTGAA GGCGCTCCCC CTCTCTGCGA CATGCATCCC 
    ATGAGAGCCC TGTTTCTCAT CCCCCGGAAT CCTCCTCCCC GGCTGAAGTC CAAAAAATGG 
    TCCAAGAAGT TTTTTAGTTT TATAGAAGGA TGCCTGGTGA AGAATTACAT GCAGCGGCCC 
    TCCACCGAGC AGCTTCTGAA GCATCCTTTC ATTAGGGATC AGCCGAACGA AAGGCAGGTT 
    AGAATCCAGC TTAAGGACCA TATAGACCGG ACCAGGAAGA AGAGAGGAGA GAAAGATGAA 
    ACCGAGTATG AGTACAGTGG GAGTGAGGAA GAGGAGGAGG AGGCGCCTGA GCAGGAGGGC 
    GAGCCCAGCT CCATCGTCAA CGTGCCGGGG GAGTCGACGC TGCGCCGGGA CTTCCTGCGG 
    CTCCAGCAGG AGAACAAGGA GCGCTCCGAG GCCCTGAGGA GGCAGCAGCT CCTGCAGGAG 
    CAGCAGCTCC GCGAGCAGGA GGAGTATAAA CGGCAGCTGC TGGCGGAGAG GCAGAAGCGG 
    ATCGAGCAGC AGAAGGAGCA GAGGCGGCGG CTGGAAGAGC AACAGAGGAG AGAGCGGGAA 
    GCCAGAAGGC AGCAGGAACG TGAGCAGCGG AGGAGGGAAC AGGAGGAGAA GAGGCGGCTG 
    GAGGAGCTGG AGAGACGACG GAAGGAGGAG GAGGAGCGGA GGCGGGCGGA GGAGGAGAAG 
    AGGAGAGTGG AGAGGGAGCA GGAATACATC CGACGGCAGC TGGAAGAGGA ACAGCGTCAC 
    CTGGAACTGC TTCAGCAGCA GCTGCTCCAG GAGCAGGCCA TGCTACTGGA GTGCCGCTGG 
    CGGGAGCTGG AGGAGCACCG GCAGGCAGAG CGGCTCCAGA GGCAGCTGCA GCAAGAACAA 
    GCCTATCTCC TGTCTCTACA GCACGACCCC CGGAGGCCGC CCCCGCCGCC AGACAGGAGC 
    AAGGCTGGCC TGCACGAGCC CAAGGCCCCG CACGAGCCCG CTGACCGGGC CCGCGAGGTG 
    GAAGATAGAT TTAGGAAAAC TAACCACAGC TCCCCCGAAG CCCAGTCTAA GCAGACGGGC 
    CGAGTATTGG AACCTCCAGT GCCTTCCAGA TCAGAGTCTT TTTCCAGTGG CAGCTCCGAG 
    TCTGTGCACC CTGCCCTGCA GAGACCCGCC GAGCCACAGG TACAGTGGTC CCACCTGGCA 
    TCTCTCAAGA ACAATGTTTC CCCTGTCTCG AGATCCCATT CCTTCAGTGA CCCTTCTCCT 
    CCCAAATTTG CACACCACCA TCTTCGCTCT CAGGACCCCT GCCCACCTTC CCGCAGTGAG 
    GTGCTCAGTC AGAGTTCTGA CTCTAAGGCG GAGGTGCCCG ACCCAGCCCA GAAGGCTTGG 
    TCTAGATCAG ACAGTGACGA GGTGCCTCCA AGGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCAAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCAGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC GCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA CGTGCGGCCG CCGCACAAGG TGACGGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACGAC GGACGAGGAG GACGACGACG CGGAGCAGGA AGGGGCCGAG 
    GAGCCCACGT CTGGCCCGGA GGACACCAGA GCCGCATCAT CTCTGAACTT GAGCAACGGT 
    GAAACGGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCCGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CTAACACTCG GCCCCAGAGC GACACGCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CTGAGATCCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACT 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTGGT GACGATATCT 
    GGGAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCAG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
    TTTGGCGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCGGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGC 
    AGCATCAAAC CCCACGCGAT CATCATCCTC CCCAACACAG ACGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTAAAC ACGTATGGGA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAGATGCC GACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCACAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
    GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260732.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116517.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OBu45787
    Clone ID Related Accession (Same CDS sequence) XM_025260732.1
    Accession Version XM_025260732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116517.1
    CDS125..4180
    Translation

    Target ORF information:

    RefSeq Version XM_025260732.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260733.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116518.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OBu45788
    Clone ID Related Accession (Same CDS sequence) XM_025260733.1
    Accession Version XM_025260733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4014bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCGTGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCGT TTGGCGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCGGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGCA GCATCAAACC CCACGCGATC 
    ATCATCCTCC CCAACACAGA CGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTAAACA CGTATGGGAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCG 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCACAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116518.1
    CDS126..4139
    Translation

    Target ORF information:

    RefSeq Version XM_025260733.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCGTGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCGT TTGGCGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCGGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGCA GCATCAAACC CCACGCGATC 
    ATCATCCTCC CCAACACAGA CGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTAAACA CGTATGGGAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCG 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCACAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260734.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116519.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.
    Price & Availability↑
    CloneID OBu45789
    Clone ID Related Accession (Same CDS sequence) XM_025260734.1
    Accession Version XM_025260734.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTCCC TGTGAGAACA 
    ACGTCACGGT CCCCCGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
    AGTAACCAAG CTGGTCAGAG AAACTCCACC AGTAGCATCG AGCCAAGGCT GCTCTGGGAG 
    AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCA 
    GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG 
    AGATCATCAT CCAAATCTGA AGGCTCGCCA TCTCAGCGCC TTGAAAACGC AGCAAAAAAG 
    CCTGAAGATA AAAAGGAAGT GTTTAGACCT CTCAAGCCTG CTGGTGAAGT GGACCTGACG 
    GCGCTGGCCA AAGAGCTGCG GGCCGTGGAG GACGTGCGGC CGCCGCACAA GGTGACGGAC 
    TACTCCTCCT CCAGCGAGGA GTCGGGGACG ACGGACGAGG AGGACGACGA CGCGGAGCAG 
    GAAGGGGCCG AGGAGCCCAC GTCTGGCCCG GAGGACACCA GAGCCGCATC ATCTCTGAAC 
    TTGAGCAACG GTGAAACGGA GTCCGTGAAA ACCATGGTTG TCCATGACGA CGTGGAGAGT 
    GAACCAGCCC TGACCCCGTC CAAAGAGGGC ACGCTGATCG TCCGCCAGAG TGCAGTTGAC 
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCTTC CCCATCCTCC 
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG 
    ACTCAGTCCG CTAGTAACAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGCGGGA CAACAGTGAC TTCTGTGGTG 
    GGGTTTTCCT GTGATGGAAT GAGACCCGAG GCCATGAGGC AAGATCCTAC GCGGAAGGGC 
    TCAGTGGTCA ATGTCAATCC CACTAACACT CGGCCCCAGA GCGACACGCC AGAGATTCGC 
    AAATACAAGA AGAGGTTTAA CTCTGAGATC CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
    TTGGTGGGTA CTGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
    CCTCTGATCA ACAGAAGGCG ATTTCAGCAG ATGGACGTCC TGGAAGGCTT GAACGTCTTG 
    GTGACGATAT CTGGGAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
    AAAATACTTC ACAACGACCC AGAGGTTGAG AAGAAGCAGG GCTGGACGAC AGTGGGGGAC 
    CTGGAAGGCT GTGTGCATTA TAAAGTTGTA AAATATGAAA GGATCAAATT TCTGGTCATT 
    GCTTTGAAGA ACTCTGTGGA AGTCTACGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
    GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC AAGCCGTTGC TGGTGGATCT CACTGTTGAG 
    GAAGGCCAGA GGCTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
    GACTCGGGAT CGGTCTATGA CATTTACCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
    ATGATCCAAT GCAGCATCAA ACCCCACGCG ATCATCATCC TCCCCAACAC AGACGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTAA ACACGTATGG GAGGATCACC 
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCGACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCACAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGCAATGAC 
    AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116519.1
    CDS124..4110
    Translation

    Target ORF information:

    RefSeq Version XM_025260734.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260734.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTACAGTGG 
    TCCCACCTGG CATCTCTCAA GAACAATGTT TCCCCTGTCT CGAGATCCCA TTCCTTCAGT 
    GACCCTTCTC CTCCCAAATT TGCACACCAC CATCTTCGCT CTCAGGACCC CTGCCCACCT 
    TCCCGCAGTG AGGTGCTCAG TCAGAGTTCT GACTCTAAGG CGGAGGTGCC CGACCCAGCC 
    CAGAAGGCTT GGTCTAGATC AGACAGTGAC GAGGTGCCTC CAAGGGTCCC TGTGAGAACA 
    ACGTCACGGT CCCCCGTCCT GTCCCGCCGG GATTCCCCAC TGCAGGGCAG TGGGCAGCAG 
    AGTAACCAAG CTGGTCAGAG AAACTCCACC AGTAGCATCG AGCCAAGGCT GCTCTGGGAG 
    AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC AGTGGCAGCT CCTCGGGGTC CAGCAACTCA 
    GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG 
    AGATCATCAT CCAAATCTGA AGGCTCGCCA TCTCAGCGCC TTGAAAACGC AGCAAAAAAG 
    CCTGAAGATA AAAAGGAAGT GTTTAGACCT CTCAAGCCTG CTGGTGAAGT GGACCTGACG 
    GCGCTGGCCA AAGAGCTGCG GGCCGTGGAG GACGTGCGGC CGCCGCACAA GGTGACGGAC 
    TACTCCTCCT CCAGCGAGGA GTCGGGGACG ACGGACGAGG AGGACGACGA CGCGGAGCAG 
    GAAGGGGCCG AGGAGCCCAC GTCTGGCCCG GAGGACACCA GAGCCGCATC ATCTCTGAAC 
    TTGAGCAACG GTGAAACGGA GTCCGTGAAA ACCATGGTTG TCCATGACGA CGTGGAGAGT 
    GAACCAGCCC TGACCCCGTC CAAAGAGGGC ACGCTGATCG TCCGCCAGAG TGCAGTTGAC 
    CAAAAGCGCG CCAGCCATCA TGAGAGCAAT GGCTTTGCGG GTCGCATTCA CCTCTTGCCA 
    GATCTCTTAC AGCAAAGCCA TTCCTCCTCC ACTTCCTCCA CCTCCTCTTC CCCATCCTCC 
    AGCCAGCCGA CACCCACCAT GTCCCCACAG ACACCCCAGG ACAAGCTCAC TACTAATGAG 
    ACTCAGTCCG CTAGTAACAC ACTCCAGAAA CACAAATCTT CCTCCTCCTT TACACCTTTT 
    ATAGACCCCA GATTACTACA GATTTCTCCA TCTAGCGGGA CAACAGTGAC TTCTGTGGTG 
    GGGTTTTCCT GTGATGGAAT GAGACCCGAG GCCATGAGGC AAGATCCTAC GCGGAAGGGC 
    TCAGTGGTCA ATGTCAATCC CACTAACACT CGGCCCCAGA GCGACACGCC AGAGATTCGC 
    AAATACAAGA AGAGGTTTAA CTCTGAGATC CTGTGCGCTG CCTTGTGGGG AGTGAACTTG 
    TTGGTGGGTA CTGAGAGTGG CCTGATGCTG CTGGACAGAA GTGGCCAAGG GAAGGTGTAT 
    CCTCTGATCA ACAGAAGGCG ATTTCAGCAG ATGGACGTCC TGGAAGGCTT GAACGTCTTG 
    GTGACGATAT CTGGGAAAAA GGATAAGTTA CGTGTCTACT ATTTGTCCTG GTTAAGAAAT 
    AAAATACTTC ACAACGACCC AGAGGTTGAG AAGAAGCAGG GCTGGACGAC AGTGGGGGAC 
    CTGGAAGGCT GTGTGCATTA TAAAGTTGTA AAATATGAAA GGATCAAATT TCTGGTCATT 
    GCTTTGAAGA ACTCTGTGGA AGTCTACGCG TGGGCACCCA AGCCATATCA CAAATTCATG 
    GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC AAGCCGTTGC TGGTGGATCT CACTGTTGAG 
    GAAGGCCAGA GGCTGAAAGT GATCTATGGA TCCTGTGCTG GATTCCATGC TGTTGATGTG 
    GACTCGGGAT CGGTCTATGA CATTTACCTA CCAACACACG TAAGAAAGAA CCCACACTCT 
    ATGATCCAAT GCAGCATCAA ACCCCACGCG ATCATCATCC TCCCCAACAC AGACGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTAA ACACGTATGG GAGGATCACC 
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCGACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCACAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGCAATGAC 
    AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260735.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116520.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.
    Price & Availability↑
    CloneID OBu45790
    Clone ID Related Accession (Same CDS sequence) XM_025260735.1
    Accession Version XM_025260735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACAGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTGC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC ATACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCGGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGCG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCCGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGAGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAATACATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGCCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGCGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTATTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCCAGTGGC 
    AGCTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTCCCTGT GAGAACAACG 
    TCACGGTCCC CCGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAGT 
    AACCAAGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CAAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCAGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCG 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC GTGCGGCCGC CGCACAAGGT GACGGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACGACG GACGAGGAGG ACGACGACGC GGAGCAGGAA 
    GGGGCCGAGG AGCCCACGTC TGGCCCGGAG GACACCAGAG CCGCATCATC TCTGAACTTG 
    AGCAACGGTG AAACGGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCCGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC TAACACTCGG CCCCAGAGCG ACACGCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC TGAGATCCTG TGCGCTGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACTG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTGGTG 
    ACGATATCTG GGAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCAGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCGTGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCGT TTGGCGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCGGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGCA GCATCAAACC CCACGCGATC ATCATCCTCC CCAACACAGA CGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTAAACA CGTATGGGAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAGATGCCG ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCACAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG CAATGACAAG 
    GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116520.1
    CDS63..4046
    Translation

    Target ORF information:

    RefSeq Version XM_025260735.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGATGTTA CCGAGGATGA AGAGGAGGAA ATCAAACTGG AGATAAATAT GCTGAAGAAA 
    TATTCTCATC ATAGAAATAT TGCAACGTAT TACGGTGCTT TCATTAAAAA GAGCCCCCCA 
    GGACACGATG ACCAGCTTTG GCTCGTTATG GAGTTCTGTG GGGCCGGATC CATCACAGAC 
    CTGGTGAAGA ACACCAAGGG CAACACGCTA AAGGAAGACT GGATAGCCTA CATCTCCAGG 
    GAGATCCTGC GGGGCCTGGC CCATCTCCAC ATCCATCACG TGATCCACCG CGACATCAAG 
    GGCCAGAATG TGCTGCTGAC CGAGAACGCA GAGGTGAAGC TGGTGGACTT CGGGGTGAGT 
    GCCCAGCTGG ACAGGACCGT GGGGAGGAGG AACACGTTCA TAGGCACCCC ATACTGGATG 
    GCCCCTGAGG TCATTGCCTG TGACGAGAAC CCGGACGCCA CCTACGATTA CAGGAGTGAC 
    CTCTGGTCCT GTGGCATCAC TGCCATTGAG ATGGCTGAAG GCGCTCCCCC TCTCTGCGAC 
    ATGCATCCCA TGAGAGCCCT GTTTCTCATC CCCCGGAATC CTCCTCCCCG GCTGAAGTCC 
    AAAAAATGGT CCAAGAAGTT TTTTAGTTTT ATAGAAGGAT GCCTGGTGAA GAATTACATG 
    CAGCGGCCCT CCACCGAGCA GCTTCTGAAG CATCCTTTCA TTAGGGATCA GCCGAACGAA 
    AGGCAGGTTA GAATCCAGCT TAAGGACCAT ATAGACCGGA CCAGGAAGAA GAGAGGAGAG 
    AAAGATGAAA CCGAGTATGA GTACAGTGGG AGTGAGGAAG AGGAGGAGGA GGCGCCTGAG 
    CAGGAGGGCG AGCCCAGCTC CATCGTCAAC GTGCCGGGGG AGTCGACGCT GCGCCGGGAC 
    TTCCTGCGGC TCCAGCAGGA GAACAAGGAG CGCTCCGAGG CCCTGAGGAG GCAGCAGCTC 
    CTGCAGGAGC AGCAGCTCCG CGAGCAGGAG GAGTATAAAC GGCAGCTGCT GGCGGAGAGG 
    CAGAAGCGGA TCGAGCAGCA GAAGGAGCAG AGGCGGCGGC TGGAAGAGCA ACAGAGGAGA 
    GAGCGGGAAG CCAGAAGGCA GCAGGAACGT GAGCAGCGGA GGAGGGAACA GGAGGAGAAG 
    AGGCGGCTGG AGGAGCTGGA GAGACGACGG AAGGAGGAGG AGGAGCGGAG GCGGGCGGAG 
    GAGGAGAAGA GGAGAGTGGA GAGGGAGCAG GAATACATCC GACGGCAGCT GGAAGAGGAA 
    CAGCGTCACC TGGAACTGCT TCAGCAGCAG CTGCTCCAGG AGCAGGCCAT GCTACTGGAG 
    TGCCGCTGGC GGGAGCTGGA GGAGCACCGG CAGGCAGAGC GGCTCCAGAG GCAGCTGCAG 
    CAAGAACAAG CCTATCTCCT GTCTCTACAG CACGACCCCC GGAGGCCGCC CCCGCCGCCA 
    GACAGGAGCA AGGCTGGCCT GCACGAGCCC AAGGCCCCGC ACGAGCCCGC TGACCGGGCC 
    CGCGAGGTGG AAGATAGATT TAGGAAAACT AACCACAGCT CCCCCGAAGC CCAGTCTAAG 
    CAGACGGGCC GAGTATTGGA ACCTCCAGTG CCTTCCAGAT CAGAGTCTTT TTCCAGTGGC 
    AGCTCCGAGT CTGTGCACCC TGCCCTGCAG AGACCCGCCG AGCCACAGGT ACAGTGGTCC 
    CACCTGGCAT CTCTCAAGAA CAATGTTTCC CCTGTCTCGA GATCCCATTC CTTCAGTGAC 
    CCTTCTCCTC CCAAATTTGC ACACCACCAT CTTCGCTCTC AGGACCCCTG CCCACCTTCC 
    CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG 
    AAGGCTTGGT CTAGATCAGA CAGTGACGAG GTGCCTCCAA GGGTCCCTGT GAGAACAACG 
    TCACGGTCCC CCGTCCTGTC CCGCCGGGAT TCCCCACTGC AGGGCAGTGG GCAGCAGAGT 
    AACCAAGCTG GTCAGAGAAA CTCCACCAGT AGCATCGAGC CAAGGCTGCT CTGGGAGAGA 
    GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT GGCAGCTCCT CGGGGTCCAG CAACTCAGGG 
    TCCCAGCCCG GCTCCCACCC AGGGTCTCAG AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA 
    TCATCATCCA AATCTGAAGG CTCGCCATCT CAGCGCCTTG AAAACGCAGC AAAAAAGCCT 
    GAAGATAAAA AGGAAGTGTT TAGACCTCTC AAGCCTGCTG GTGAAGTGGA CCTGACGGCG 
    CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC GTGCGGCCGC CGCACAAGGT GACGGACTAC 
    TCCTCCTCCA GCGAGGAGTC GGGGACGACG GACGAGGAGG ACGACGACGC GGAGCAGGAA 
    GGGGCCGAGG AGCCCACGTC TGGCCCGGAG GACACCAGAG CCGCATCATC TCTGAACTTG 
    AGCAACGGTG AAACGGAGTC CGTGAAAACC ATGGTTGTCC ATGACGACGT GGAGAGTGAA 
    CCAGCCCTGA CCCCGTCCAA AGAGGGCACG CTGATCGTCC GCCAGAGTGC AGTTGACCAA 
    AAGCGCGCCA GCCATCATGA GAGCAATGGC TTTGCGGGTC GCATTCACCT CTTGCCAGAT 
    CTCTTACAGC AAAGCCATTC CTCCTCCACT TCCTCCACCT CCTCTTCCCC ATCCTCCAGC 
    CAGCCGACAC CCACCATGTC CCCACAGACA CCCCAGGACA AGCTCACTAC TAATGAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCCGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC TAACACTCGG CCCCAGAGCG ACACGCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC TGAGATCCTG TGCGCTGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACTG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTGGTG 
    ACGATATCTG GGAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCAGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCGTGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCGT TTGGCGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCGGGATCGG TCTATGACAT TTACCTACCA ACACACGTAA GAAAGAACCC ACACTCTATG 
    ATCCAATGCA GCATCAAACC CCACGCGATC ATCATCCTCC CCAACACAGA CGGCATGGAG 
    CTCCTGGTGT GCTATGAAGA TGAAGGGGTT TACGTAAACA CGTATGGGAG GATCACCAAG 
    GATGTGGTTC TGCAGTGGGG AGAGATGCCG ACGTCTGTAG CATATATTCG ATCCAATCAG 
    ACGATGGGCT GGGGAGAAAA GGCCATAGAG ATCCGATCTG TGGAAACTGG CCACTTGGAT 
    GGCGTGTTTA TGCACAAAAG GGCTCAAAGG CTGAAGTTCT TGTGTGAACG CAATGACAAG 
    GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC AGCAGCCAGG TTTACTTCAT GACTCTCGGC 
    AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260736.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116521.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.
    Price & Availability↑
    CloneID OBu45791
    Clone ID Related Accession (Same CDS sequence) XM_025260736.1
    Accession Version XM_025260736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GTGGTCCCAC 
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCCTGCCC ACCTTCCCGC 
    AGTGAGGTGC TCAGTCAGAG TTCTGACTCT AAGGCGGAGG TGCCCGACCC AGCCCAGAAG 
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TCCCTGTGAG AACAACGTCA 
    CGGTCCCCCG TCCTGTCCCG CCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAGAGTAAC 
    CAAGCTGGTC AGAGAAACTC CACCAGTAGC ATCGAGCCAA GGCTGCTCTG GGAGAGAGTG 
    GAGAAGCTGG CGCCCAGGCC AGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCAGGGTCC 
    CAGCCCGGCT CCCACCCAGG GTCTCAGAGT GGCTCCGGGG AGCGCTTCAG AGTGAGATCA 
    TCATCCAAAT CTGAAGGCTC GCCATCTCAG CGCCTTGAAA ACGCAGCAAA AAAGCCTGAA 
    GATAAAAAGG AAGTGTTTAG ACCTCTCAAG CCTGCTGGTG AAGTGGACCT GACGGCGCTG 
    GCCAAAGAGC TGCGGGCCGT GGAGGACGTG CGGCCGCCGC ACAAGGTGAC GGACTACTCC 
    TCCTCCAGCG AGGAGTCGGG GACGACGGAC GAGGAGGACG ACGACGCGGA GCAGGAAGGG 
    GCCGAGGAGC CCACGTCTGG CCCGGAGGAC ACCAGAGCCG CATCATCTCT GAACTTGAGC 
    AACGGTGAAA CGGAGTCCGT GAAAACCATG GTTGTCCATG ACGACGTGGA GAGTGAACCA 
    GCCCTGACCC CGTCCAAAGA GGGCACGCTG ATCGTCCGCC AGAGTGCAGT TGACCAAAAG 
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CTTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC CGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACTAA CACTCGGCCC CAGAGCGACA CGCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCTGA GATCCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACTGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTGGTGACG 
    ATATCTGGGA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCAGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCGTTTG GCGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCG 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
    CAATGCAGCA TCAAACCCCA CGCGATCATC ATCCTCCCCA ACACAGACGG CATGGAGCTC 
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAC GTAAACACGT ATGGGAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA GATGCCGACG TCTGTAGCAT ATATTCGATC CAATCAGACG 
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGCCA CTTGGATGGC 
    GTGTTTATGC ACAAAAGGGC TCAAAGGCTG AAGTTCTTGT GTGAACGCAA TGACAAGGTG 
    TTCTTTGCCT CGGTCCGGTC TGGGGGCAGC AGCCAGGTTT ACTTCATGAC TCTCGGCAGG 
    ACCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116521.1
    CDS124..4104
    Translation

    Target ORF information:

    RefSeq Version XM_025260736.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GTGGTCCCAC 
    CTGGCATCTC TCAAGAACAA TGTTTCCCCT GTCTCGAGAT CCCATTCCTT CAGTGACCCT 
    TCTCCTCCCA AATTTGCACA CCACCATCTT CGCTCTCAGG ACCCCTGCCC ACCTTCCCGC 
    AGTGAGGTGC TCAGTCAGAG TTCTGACTCT AAGGCGGAGG TGCCCGACCC AGCCCAGAAG 
    GCTTGGTCTA GATCAGACAG TGACGAGGTG CCTCCAAGGG TCCCTGTGAG AACAACGTCA 
    CGGTCCCCCG TCCTGTCCCG CCGGGATTCC CCACTGCAGG GCAGTGGGCA GCAGAGTAAC 
    CAAGCTGGTC AGAGAAACTC CACCAGTAGC ATCGAGCCAA GGCTGCTCTG GGAGAGAGTG 
    GAGAAGCTGG CGCCCAGGCC AGGCAGTGGC AGCTCCTCGG GGTCCAGCAA CTCAGGGTCC 
    CAGCCCGGCT CCCACCCAGG GTCTCAGAGT GGCTCCGGGG AGCGCTTCAG AGTGAGATCA 
    TCATCCAAAT CTGAAGGCTC GCCATCTCAG CGCCTTGAAA ACGCAGCAAA AAAGCCTGAA 
    GATAAAAAGG AAGTGTTTAG ACCTCTCAAG CCTGCTGGTG AAGTGGACCT GACGGCGCTG 
    GCCAAAGAGC TGCGGGCCGT GGAGGACGTG CGGCCGCCGC ACAAGGTGAC GGACTACTCC 
    TCCTCCAGCG AGGAGTCGGG GACGACGGAC GAGGAGGACG ACGACGCGGA GCAGGAAGGG 
    GCCGAGGAGC CCACGTCTGG CCCGGAGGAC ACCAGAGCCG CATCATCTCT GAACTTGAGC 
    AACGGTGAAA CGGAGTCCGT GAAAACCATG GTTGTCCATG ACGACGTGGA GAGTGAACCA 
    GCCCTGACCC CGTCCAAAGA GGGCACGCTG ATCGTCCGCC AGAGTGCAGT TGACCAAAAG 
    CGCGCCAGCC ATCATGAGAG CAATGGCTTT GCGGGTCGCA TTCACCTCTT GCCAGATCTC 
    TTACAGCAAA GCCATTCCTC CTCCACTTCC TCCACCTCCT CTTCCCCATC CTCCAGCCAG 
    CCGACACCCA CCATGTCCCC ACAGACACCC CAGGACAAGC TCACTACTAA TGAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC CGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACTAA CACTCGGCCC CAGAGCGACA CGCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCTGA GATCCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACTGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTGGTGACG 
    ATATCTGGGA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCAGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCGTTTG GCGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCG 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACGTAAGAA AGAACCCACA CTCTATGATC 
    CAATGCAGCA TCAAACCCCA CGCGATCATC ATCCTCCCCA ACACAGACGG CATGGAGCTC 
    CTGGTGTGCT ATGAAGATGA AGGGGTTTAC GTAAACACGT ATGGGAGGAT CACCAAGGAT 
    GTGGTTCTGC AGTGGGGAGA GATGCCGACG TCTGTAGCAT ATATTCGATC CAATCAGACG 
    ATGGGCTGGG GAGAAAAGGC CATAGAGATC CGATCTGTGG AAACTGGCCA CTTGGATGGC 
    GTGTTTATGC ACAAAAGGGC TCAAAGGCTG AAGTTCTTGT GTGAACGCAA TGACAAGGTG 
    TTCTTTGCCT CGGTCCGGTC TGGGGGCAGC AGCCAGGTTT ACTTCATGAC TCTCGGCAGG 
    ACCTCTCTTC TGAGCTGGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260737.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116522.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.
    Price & Availability↑
    CloneID OBu45792
    Clone ID Related Accession (Same CDS sequence) XM_025260737.1
    Accession Version XM_025260737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCCGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACTAACACT 
    CGGCCCCAGA GCGACACGCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCTGAGATC 
    CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CTGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTG GTGACGATAT CTGGGAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC AGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCG 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCGGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAAT GCAGCATCAA ACCCCACGCG 
    ATCATCATCC TCCCCAACAC AGACGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTAA ACACGTATGG GAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
    CCGACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCACAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116522.1
    CDS125..4081
    Translation

    Target ORF information:

    RefSeq Version XM_025260737.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCCGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACTAACACT 
    CGGCCCCAGA GCGACACGCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCTGAGATC 
    CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CTGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTG GTGACGATAT CTGGGAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC AGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCG 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCGGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACG TAAGAAAGAA CCCACACTCT ATGATCCAAT GCAGCATCAA ACCCCACGCG 
    ATCATCATCC TCCCCAACAC AGACGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTAA ACACGTATGG GAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
    CCGACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCACAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260738.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116523.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.
    Price & Availability↑
    CloneID OBu45793
    Clone ID Related Accession (Same CDS sequence) XM_025260738.1
    Accession Version XM_025260738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3936bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTGAAGA AATATTCTCA TCATAGAAAT ATTGCAACGT ATTACGGTGC TTTCATTAAA 
    AAGAGCCCCC CAGGACACGA TGACCAGCTT TGGCTCGTTA TGGAGTTCTG TGGGGCCGGA 
    TCCATCACAG ACCTGGTGAA GAACACCAAG GGCAACACGC TAAAGGAAGA CTGGATAGCC 
    TACATCTCCA GGGAGATCCT GCGGGGCCTG GCCCATCTCC ACATCCATCA CGTGATCCAC 
    CGCGACATCA AGGGCCAGAA TGTGCTGCTG ACCGAGAACG CAGAGGTGAA GCTGGTGGAC 
    TTCGGGGTGA GTGCCCAGCT GGACAGGACC GTGGGGAGGA GGAACACGTT CATAGGCACC 
    CCATACTGGA TGGCCCCTGA GGTCATTGCC TGTGACGAGA ACCCGGACGC CACCTACGAT 
    TACAGGAGTG ACCTCTGGTC CTGTGGCATC ACTGCCATTG AGATGGCTGA AGGCGCTCCC 
    CCTCTCTGCG ACATGCATCC CATGAGAGCC CTGTTTCTCA TCCCCCGGAA TCCTCCTCCC 
    CGGCTGAAGT CCAAAAAATG GTCCAAGAAG TTTTTTAGTT TTATAGAAGG ATGCCTGGTG 
    AAGAATTACA TGCAGCGGCC CTCCACCGAG CAGCTTCTGA AGCATCCTTT CATTAGGGAT 
    CAGCCGAACG AAAGGCAGGT TAGAATCCAG CTTAAGGACC ATATAGACCG GACCAGGAAG 
    AAGAGAGGAG AGAAAGATGA AACCGAGTAT GAGTACAGTG GGAGTGAGGA AGAGGAGGAG 
    GAGGCGCCTG AGCAGGAGGG CGAGCCCAGC TCCATCGTCA ACGTGCCGGG GGAGTCGACG 
    CTGCGCCGGG ACTTCCTGCG GCTCCAGCAG GAGAACAAGG AGCGCTCCGA GGCCCTGAGG 
    AGGCAGCAGC TCCTGCAGGA GCAGCAGCTC CGCGAGCAGG AGGAGTATAA ACGGCAGCTG 
    CTGGCGGAGA GGCAGAAGCG GATCGAGCAG CAGAAGGAGC AGAGGCGGCG GCTGGAAGAG 
    CAACAGAGGA GAGAGCGGGA AGCCAGAAGG CAGCAGGAAC GTGAGCAGCG GAGGAGGGAA 
    CAGGAGGAGA AGAGGCGGCT GGAGGAGCTG GAGAGACGAC GGAAGGAGGA GGAGGAGCGG 
    AGGCGGGCGG AGGAGGAGAA GAGGAGAGTG GAGAGGGAGC AGGAATACAT CCGACGGCAG 
    CTGGAAGAGG AACAGCGTCA CCTGGAACTG CTTCAGCAGC AGCTGCTCCA GGAGCAGGCC 
    ATGCTACTGG AGTGCCGCTG GCGGGAGCTG GAGGAGCACC GGCAGGCAGA GCGGCTCCAG 
    AGGCAGCTGC AGCAAGAACA AGCCTATCTC CTGTCTCTAC AGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116523.1
    CDS1265..5200
    Translation

    Target ORF information:

    RefSeq Version XM_025260738.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTGAAGA AATATTCTCA TCATAGAAAT ATTGCAACGT ATTACGGTGC TTTCATTAAA 
    AAGAGCCCCC CAGGACACGA TGACCAGCTT TGGCTCGTTA TGGAGTTCTG TGGGGCCGGA 
    TCCATCACAG ACCTGGTGAA GAACACCAAG GGCAACACGC TAAAGGAAGA CTGGATAGCC 
    TACATCTCCA GGGAGATCCT GCGGGGCCTG GCCCATCTCC ACATCCATCA CGTGATCCAC 
    CGCGACATCA AGGGCCAGAA TGTGCTGCTG ACCGAGAACG CAGAGGTGAA GCTGGTGGAC 
    TTCGGGGTGA GTGCCCAGCT GGACAGGACC GTGGGGAGGA GGAACACGTT CATAGGCACC 
    CCATACTGGA TGGCCCCTGA GGTCATTGCC TGTGACGAGA ACCCGGACGC CACCTACGAT 
    TACAGGAGTG ACCTCTGGTC CTGTGGCATC ACTGCCATTG AGATGGCTGA AGGCGCTCCC 
    CCTCTCTGCG ACATGCATCC CATGAGAGCC CTGTTTCTCA TCCCCCGGAA TCCTCCTCCC 
    CGGCTGAAGT CCAAAAAATG GTCCAAGAAG TTTTTTAGTT TTATAGAAGG ATGCCTGGTG 
    AAGAATTACA TGCAGCGGCC CTCCACCGAG CAGCTTCTGA AGCATCCTTT CATTAGGGAT 
    CAGCCGAACG AAAGGCAGGT TAGAATCCAG CTTAAGGACC ATATAGACCG GACCAGGAAG 
    AAGAGAGGAG AGAAAGATGA AACCGAGTAT GAGTACAGTG GGAGTGAGGA AGAGGAGGAG 
    GAGGCGCCTG AGCAGGAGGG CGAGCCCAGC TCCATCGTCA ACGTGCCGGG GGAGTCGACG 
    CTGCGCCGGG ACTTCCTGCG GCTCCAGCAG GAGAACAAGG AGCGCTCCGA GGCCCTGAGG 
    AGGCAGCAGC TCCTGCAGGA GCAGCAGCTC CGCGAGCAGG AGGAGTATAA ACGGCAGCTG 
    CTGGCGGAGA GGCAGAAGCG GATCGAGCAG CAGAAGGAGC AGAGGCGGCG GCTGGAAGAG 
    CAACAGAGGA GAGAGCGGGA AGCCAGAAGG CAGCAGGAAC GTGAGCAGCG GAGGAGGGAA 
    CAGGAGGAGA AGAGGCGGCT GGAGGAGCTG GAGAGACGAC GGAAGGAGGA GGAGGAGCGG 
    AGGCGGGCGG AGGAGGAGAA GAGGAGAGTG GAGAGGGAGC AGGAATACAT CCGACGGCAG 
    CTGGAAGAGG AACAGCGTCA CCTGGAACTG CTTCAGCAGC AGCTGCTCCA GGAGCAGGCC 
    ATGCTACTGG AGTGCCGCTG GCGGGAGCTG GAGGAGCACC GGCAGGCAGA GCGGCTCCAG 
    AGGCAGCTGC AGCAAGAACA AGCCTATCTC CTGTCTCTAC AGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGAGT 
    GCAGTTGACC AAAAGCGCGC CAGCCATCAT GAGAGCAATG GCTTTGCGGG TCGCATTCAC 
    CTCTTGCCAG ATCTCTTACA GCAAAGCCAT TCCTCCTCCA CTTCCTCCAC CTCCTCTTCC 
    CCATCCTCCA GCCAGCCGAC ACCCACCATG TCCCCACAGA CACCCCAGGA CAAGCTCACT 
    ACTAATGAGA CTCAGTCCGC TAGTAACACA CTCCAGAAAC ACAAATCTTC CTCCTCCTTT 
    ACACCTTTTA TAGACCCCAG ATTACTACAG ATTTCTCCAT CTAGCGGGAC AACAGTGACT 
    TCTGTGGTGG GGTTTTCCTG TGATGGAATG AGACCCGAGG CCATGAGGCA AGATCCTACG 
    CGGAAGGGCT CAGTGGTCAA TGTCAATCCC ACTAACACTC GGCCCCAGAG CGACACGCCA 
    GAGATTCGCA AATACAAGAA GAGGTTTAAC TCTGAGATCC TGTGCGCTGC CTTGTGGGGA 
    GTGAACTTGT TGGTGGGTAC TGAGAGTGGC CTGATGCTGC TGGACAGAAG TGGCCAAGGG 
    AAGGTGTATC CTCTGATCAA CAGAAGGCGA TTTCAGCAGA TGGACGTCCT GGAAGGCTTG 
    AACGTCTTGG TGACGATATC TGGGAAAAAG GATAAGTTAC GTGTCTACTA TTTGTCCTGG 
    TTAAGAAATA AAATACTTCA CAACGACCCA GAGGTTGAGA AGAAGCAGGG CTGGACGACA 
    GTGGGGGACC TGGAAGGCTG TGTGCATTAT AAAGTTGTAA AATATGAAAG GATCAAATTT 
    CTGGTCATTG CTTTGAAGAA CTCTGTGGAA GTCTACGCGT GGGCACCCAA GCCATATCAC 
    AAATTCATGG CCTTTAAGTC GTTTGGCGAG CTGGTGCACA AGCCGTTGCT GGTGGATCTC 
    ACTGTTGAGG AAGGCCAGAG GCTGAAAGTG ATCTATGGAT CCTGTGCTGG ATTCCATGCT 
    GTTGATGTGG ACTCGGGATC GGTCTATGAC ATTTACCTAC CAACACACGT AAGAAAGAAC 
    CCACACTCTA TGATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260739.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116524.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.
    Price & Availability↑
    CloneID OBu45794
    Clone ID Related Accession (Same CDS sequence) XM_025260739.1
    Accession Version XM_025260739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCCGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACTAACACT 
    CGGCCCCAGA GCGACACGCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCTGAGATC 
    CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CTGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTG GTGACGATAT CTGGGAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC AGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCG 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCGGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGCAG CATCAAACCC CACGCGATCA TCATCCTCCC CAACACAGAC 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTAAACAC GTATGGGAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCGA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCACAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGC 
    AATGACAAGG TGTTCTTTGC CTCGGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116524.1
    CDS124..4056
    Translation

    Target ORF information:

    RefSeq Version XM_025260739.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG ACTCAGTCCG CTAGTAACAC ACTCCAGAAA 
    CACAAATCTT CCTCCTCCTT TACACCTTTT ATAGACCCCA GATTACTACA GATTTCTCCA 
    TCTAGCGGGA CAACAGTGAC TTCTGTGGTG GGGTTTTCCT GTGATGGAAT GAGACCCGAG 
    GCCATGAGGC AAGATCCTAC GCGGAAGGGC TCAGTGGTCA ATGTCAATCC CACTAACACT 
    CGGCCCCAGA GCGACACGCC AGAGATTCGC AAATACAAGA AGAGGTTTAA CTCTGAGATC 
    CTGTGCGCTG CCTTGTGGGG AGTGAACTTG TTGGTGGGTA CTGAGAGTGG CCTGATGCTG 
    CTGGACAGAA GTGGCCAAGG GAAGGTGTAT CCTCTGATCA ACAGAAGGCG ATTTCAGCAG 
    ATGGACGTCC TGGAAGGCTT GAACGTCTTG GTGACGATAT CTGGGAAAAA GGATAAGTTA 
    CGTGTCTACT ATTTGTCCTG GTTAAGAAAT AAAATACTTC ACAACGACCC AGAGGTTGAG 
    AAGAAGCAGG GCTGGACGAC AGTGGGGGAC CTGGAAGGCT GTGTGCATTA TAAAGTTGTA 
    AAATATGAAA GGATCAAATT TCTGGTCATT GCTTTGAAGA ACTCTGTGGA AGTCTACGCG 
    TGGGCACCCA AGCCATATCA CAAATTCATG GCCTTTAAGT CGTTTGGCGA GCTGGTGCAC 
    AAGCCGTTGC TGGTGGATCT CACTGTTGAG GAAGGCCAGA GGCTGAAAGT GATCTATGGA 
    TCCTGTGCTG GATTCCATGC TGTTGATGTG GACTCGGGAT CGGTCTATGA CATTTACCTA 
    CCAACACACA TCCAATGCAG CATCAAACCC CACGCGATCA TCATCCTCCC CAACACAGAC 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTAAACAC GTATGGGAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCGA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCACAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGC 
    AATGACAAGG TGTTCTTTGC CTCGGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260740.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116525.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.
    Price & Availability↑
    CloneID OBu45795
    Clone ID Related Accession (Same CDS sequence) XM_025260740.1
    Accession Version XM_025260740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3915bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCAAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCAGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC GCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA CGTGCGGCCG CCGCACAAGG TGACGGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACGAC GGACGAGGAG GACGACGACG CGGAGCAGGA AGGGGCCGAG 
    GAGCCCACGT CTGGCCCGGA GGACACCAGA GCCGCATCAT CTCTGAACTT GAGCAACGGT 
    GAAACGGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCCGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CTAACACTCG GCCCCAGAGC GACACGCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CTGAGATCCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACT 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTGGT GACGATATCT 
    GGGAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCAG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
    TTTGGCGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCGGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGC 
    AGCATCAAAC CCCACGCGAT CATCATCCTC CCCAACACAG ACGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTAAAC ACGTATGGGA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAGATGCC GACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCACAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
    GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116525.1
    CDS124..4038
    Translation

    Target ORF information:

    RefSeq Version XM_025260740.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG TAGCATCGAG CCAAGGCTGC TCTGGGAGAG AGTGGAGAAG 
    CTGGCGCCCA GGCCAGGCAG TGGCAGCTCC TCGGGGTCCA GCAACTCAGG GTCCCAGCCC 
    GGCTCCCACC CAGGGTCTCA GAGTGGCTCC GGGGAGCGCT TCAGAGTGAG ATCATCATCC 
    AAATCTGAAG GCTCGCCATC TCAGCGCCTT GAAAACGCAG CAAAAAAGCC TGAAGATAAA 
    AAGGAAGTGT TTAGACCTCT CAAGCCTGCT GGTGAAGTGG ACCTGACGGC GCTGGCCAAA 
    GAGCTGCGGG CCGTGGAGGA CGTGCGGCCG CCGCACAAGG TGACGGACTA CTCCTCCTCC 
    AGCGAGGAGT CGGGGACGAC GGACGAGGAG GACGACGACG CGGAGCAGGA AGGGGCCGAG 
    GAGCCCACGT CTGGCCCGGA GGACACCAGA GCCGCATCAT CTCTGAACTT GAGCAACGGT 
    GAAACGGAGT CCGTGAAAAC CATGGTTGTC CATGACGACG TGGAGAGTGA ACCAGCCCTG 
    ACCCCGTCCA AAGAGGGCAC GCTGATCGTC CGCCAGAGTG CAGTTGACCA AAAGCGCGCC 
    AGCCATCATG AGAGCAATGG CTTTGCGGGT CGCATTCACC TCTTGCCAGA TCTCTTACAG 
    CAAAGCCATT CCTCCTCCAC TTCCTCCACC TCCTCTTCCC CATCCTCCAG CCAGCCGACA 
    CCCACCATGT CCCCACAGAC ACCCCAGGAC AAGCTCACTA CTAATGAGAC TCAGTCCGCT 
    AGTAACACAC TCCAGAAACA CAAATCTTCC TCCTCCTTTA CACCTTTTAT AGACCCCAGA 
    TTACTACAGA TTTCTCCATC TAGCGGGACA ACAGTGACTT CTGTGGTGGG GTTTTCCTGT 
    GATGGAATGA GACCCGAGGC CATGAGGCAA GATCCTACGC GGAAGGGCTC AGTGGTCAAT 
    GTCAATCCCA CTAACACTCG GCCCCAGAGC GACACGCCAG AGATTCGCAA ATACAAGAAG 
    AGGTTTAACT CTGAGATCCT GTGCGCTGCC TTGTGGGGAG TGAACTTGTT GGTGGGTACT 
    GAGAGTGGCC TGATGCTGCT GGACAGAAGT GGCCAAGGGA AGGTGTATCC TCTGATCAAC 
    AGAAGGCGAT TTCAGCAGAT GGACGTCCTG GAAGGCTTGA ACGTCTTGGT GACGATATCT 
    GGGAAAAAGG ATAAGTTACG TGTCTACTAT TTGTCCTGGT TAAGAAATAA AATACTTCAC 
    AACGACCCAG AGGTTGAGAA GAAGCAGGGC TGGACGACAG TGGGGGACCT GGAAGGCTGT 
    GTGCATTATA AAGTTGTAAA ATATGAAAGG ATCAAATTTC TGGTCATTGC TTTGAAGAAC 
    TCTGTGGAAG TCTACGCGTG GGCACCCAAG CCATATCACA AATTCATGGC CTTTAAGTCG 
    TTTGGCGAGC TGGTGCACAA GCCGTTGCTG GTGGATCTCA CTGTTGAGGA AGGCCAGAGG 
    CTGAAAGTGA TCTATGGATC CTGTGCTGGA TTCCATGCTG TTGATGTGGA CTCGGGATCG 
    GTCTATGACA TTTACCTACC AACACACGTA AGAAAGAACC CACACTCTAT GATCCAATGC 
    AGCATCAAAC CCCACGCGAT CATCATCCTC CCCAACACAG ACGGCATGGA GCTCCTGGTG 
    TGCTATGAAG ATGAAGGGGT TTACGTAAAC ACGTATGGGA GGATCACCAA GGATGTGGTT 
    CTGCAGTGGG GAGAGATGCC GACGTCTGTA GCATATATTC GATCCAATCA GACGATGGGC 
    TGGGGAGAAA AGGCCATAGA GATCCGATCT GTGGAAACTG GCCACTTGGA TGGCGTGTTT 
    ATGCACAAAA GGGCTCAAAG GCTGAAGTTC TTGTGTGAAC GCAATGACAA GGTGTTCTTT 
    GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG GTTTACTTCA TGACTCTCGG CAGGACCTCT 
    CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260742.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116527.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.
    Price & Availability↑
    CloneID OBu45796
    Clone ID Related Accession (Same CDS sequence) XM_025260742.1
    Accession Version XM_025260742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCA AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGGT GAAGTGGACC TGACGGCGCT GGCCAAAGAG 
    CTGCGGGCCG TGGAGGACGT GCGGCCGCCG CACAAGGTGA CGGACTACTC CTCCTCCAGC 
    GAGGAGTCGG GGACGACGGA CGAGGAGGAC GACGACGCGG AGCAGGAAGG GGCCGAGGAG 
    CCCACGTCTG GCCCGGAGGA CACCAGAGCC GCATCATCTC TGAACTTGAG CAACGGTGAA 
    ACGGAGTCCG TGAAAACCAT GGTTGTCCAT GACGACGTGG AGAGTGAACC AGCCCTGACC 
    CCGTCCAAAG AGGGCACGCT GATCGTCCGC CAGAGTGCAG TTGACCAAAA GCGCGCCAGC 
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCTTCCCCAT CCTCCAGCCA GCCGACACCC 
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT 
    AACACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
    CTACAGATTT CTCCATCTAG CGGGACAACA GTGACTTCTG TGGTGGGGTT TTCCTGTGAT 
    GGAATGAGAC CCGAGGCCAT GAGGCAAGAT CCTACGCGGA AGGGCTCAGT GGTCAATGTC 
    AATCCCACTA ACACTCGGCC CCAGAGCGAC ACGCCAGAGA TTCGCAAATA CAAGAAGAGG 
    TTTAACTCTG AGATCCTGTG CGCTGCCTTG TGGGGAGTGA ACTTGTTGGT GGGTACTGAG 
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TGTATCCTCT GATCAACAGA 
    AGGCGATTTC AGCAGATGGA CGTCCTGGAA GGCTTGAACG TCTTGGTGAC GATATCTGGG 
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAC 
    GACCCAGAGG TTGAGAAGAA GCAGGGCTGG ACGACAGTGG GGGACCTGGA AGGCTGTGTG 
    CATTATAAAG TTGTAAAATA TGAAAGGATC AAATTTCTGG TCATTGCTTT GAAGAACTCT 
    GTGGAAGTCT ACGCGTGGGC ACCCAAGCCA TATCACAAAT TCATGGCCTT TAAGTCGTTT 
    GGCGAGCTGG TGCACAAGCC GTTGCTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGCTG 
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGACTC GGGATCGGTC 
    TATGACATTT ACCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAATGCAGC 
    ATCAAACCCC ACGCGATCAT CATCCTCCCC AACACAGACG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTAAACACG TATGGGAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AGATGCCGAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CACAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
    TCGGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116527.1
    CDS125..4036
    Translation

    Target ORF information:

    RefSeq Version XM_025260742.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCA AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGGT GAAGTGGACC TGACGGCGCT GGCCAAAGAG 
    CTGCGGGCCG TGGAGGACGT GCGGCCGCCG CACAAGGTGA CGGACTACTC CTCCTCCAGC 
    GAGGAGTCGG GGACGACGGA CGAGGAGGAC GACGACGCGG AGCAGGAAGG GGCCGAGGAG 
    CCCACGTCTG GCCCGGAGGA CACCAGAGCC GCATCATCTC TGAACTTGAG CAACGGTGAA 
    ACGGAGTCCG TGAAAACCAT GGTTGTCCAT GACGACGTGG AGAGTGAACC AGCCCTGACC 
    CCGTCCAAAG AGGGCACGCT GATCGTCCGC CAGAGTGCAG TTGACCAAAA GCGCGCCAGC 
    CATCATGAGA GCAATGGCTT TGCGGGTCGC ATTCACCTCT TGCCAGATCT CTTACAGCAA 
    AGCCATTCCT CCTCCACTTC CTCCACCTCC TCTTCCCCAT CCTCCAGCCA GCCGACACCC 
    ACCATGTCCC CACAGACACC CCAGGACAAG CTCACTACTA ATGAGACTCA GTCCGCTAGT 
    AACACACTCC AGAAACACAA ATCTTCCTCC TCCTTTACAC CTTTTATAGA CCCCAGATTA 
    CTACAGATTT CTCCATCTAG CGGGACAACA GTGACTTCTG TGGTGGGGTT TTCCTGTGAT 
    GGAATGAGAC CCGAGGCCAT GAGGCAAGAT CCTACGCGGA AGGGCTCAGT GGTCAATGTC 
    AATCCCACTA ACACTCGGCC CCAGAGCGAC ACGCCAGAGA TTCGCAAATA CAAGAAGAGG 
    TTTAACTCTG AGATCCTGTG CGCTGCCTTG TGGGGAGTGA ACTTGTTGGT GGGTACTGAG 
    AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC CAAGGGAAGG TGTATCCTCT GATCAACAGA 
    AGGCGATTTC AGCAGATGGA CGTCCTGGAA GGCTTGAACG TCTTGGTGAC GATATCTGGG 
    AAAAAGGATA AGTTACGTGT CTACTATTTG TCCTGGTTAA GAAATAAAAT ACTTCACAAC 
    GACCCAGAGG TTGAGAAGAA GCAGGGCTGG ACGACAGTGG GGGACCTGGA AGGCTGTGTG 
    CATTATAAAG TTGTAAAATA TGAAAGGATC AAATTTCTGG TCATTGCTTT GAAGAACTCT 
    GTGGAAGTCT ACGCGTGGGC ACCCAAGCCA TATCACAAAT TCATGGCCTT TAAGTCGTTT 
    GGCGAGCTGG TGCACAAGCC GTTGCTGGTG GATCTCACTG TTGAGGAAGG CCAGAGGCTG 
    AAAGTGATCT ATGGATCCTG TGCTGGATTC CATGCTGTTG ATGTGGACTC GGGATCGGTC 
    TATGACATTT ACCTACCAAC ACACGTAAGA AAGAACCCAC ACTCTATGAT CCAATGCAGC 
    ATCAAACCCC ACGCGATCAT CATCCTCCCC AACACAGACG GCATGGAGCT CCTGGTGTGC 
    TATGAAGATG AAGGGGTTTA CGTAAACACG TATGGGAGGA TCACCAAGGA TGTGGTTCTG 
    CAGTGGGGAG AGATGCCGAC GTCTGTAGCA TATATTCGAT CCAATCAGAC GATGGGCTGG 
    GGAGAAAAGG CCATAGAGAT CCGATCTGTG GAAACTGGCC ACTTGGATGG CGTGTTTATG 
    CACAAAAGGG CTCAAAGGCT GAAGTTCTTG TGTGAACGCA ATGACAAGGT GTTCTTTGCC 
    TCGGTCCGGT CTGGGGGCAG CAGCCAGGTT TACTTCATGA CTCTCGGCAG GACCTCTCTT 
    CTGAGCTGGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260743.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116528.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.
    Price & Availability↑
    CloneID OBu45797
    Clone ID Related Accession (Same CDS sequence) XM_025260743.1
    Accession Version XM_025260743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAA GGGTCCCTGT GAGAACAACG TCACGGTCCC CCGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGCAGTGG GCAGCAGAGT AACCAAGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CAAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCAGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCG CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC 
    GTGCGGCCGC CGCACAAGGT GACGGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACGACG 
    GACGAGGAGG ACGACGACGC GGAGCAGGAA GGGGCCGAGG AGCCCACGTC TGGCCCGGAG 
    GACACCAGAG CCGCATCATC TCTGAACTTG AGCAACGGTG AAACGGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGAGTGC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC 
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC 
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
    AGCGGGACAA CAGTGACTTC TGTGGTGGGG TTTTCCTGTG ATGGAATGAG ACCCGAGGCC 
    ATGAGGCAAG ATCCTACGCG GAAGGGCTCA GTGGTCAATG TCAATCCCAC TAACACTCGG 
    CCCCAGAGCG ACACGCCAGA GATTCGCAAA TACAAGAAGA GGTTTAACTC TGAGATCCTG 
    TGCGCTGCCT TGTGGGGAGT GAACTTGTTG GTGGGTACTG AGAGTGGCCT GATGCTGCTG 
    GACAGAAGTG GCCAAGGGAA GGTGTATCCT CTGATCAACA GAAGGCGATT TCAGCAGATG 
    GACGTCCTGG AAGGCTTGAA CGTCTTGGTG ACGATATCTG GGAAAAAGGA TAAGTTACGT 
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ACGACCCAGA GGTTGAGAAG 
    AAGCAGGGCT GGACGACAGT GGGGGACCTG GAAGGCTGTG TGCATTATAA AGTTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCGTGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCGT TTGGCGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCGGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGCA GCATCAAACC CCACGCGATC 
    ATCATCCTCC CCAACACAGA CGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTAAACA CGTATGGGAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCG 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCACAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116528.1
    CDS124..4017
    Translation

    Target ORF information:

    RefSeq Version XM_025260743.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAA GGGTCCCTGT GAGAACAACG TCACGGTCCC CCGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGCAGTGG GCAGCAGAGT AACCAAGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CAAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCAGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCG CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC 
    GTGCGGCCGC CGCACAAGGT GACGGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACGACG 
    GACGAGGAGG ACGACGACGC GGAGCAGGAA GGGGCCGAGG AGCCCACGTC TGGCCCGGAG 
    GACACCAGAG CCGCATCATC TCTGAACTTG AGCAACGGTG AAACGGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGAGTGC AGTTGACCAA AAGCGCGCCA GCCATCATGA GAGCAATGGC 
    TTTGCGGGTC GCATTCACCT CTTGCCAGAT CTCTTACAGC AAAGCCATTC CTCCTCCACT 
    TCCTCCACCT CCTCTTCCCC ATCCTCCAGC CAGCCGACAC CCACCATGTC CCCACAGACA 
    CCCCAGGACA AGCTCACTAC TAATGAGACT CAGTCCGCTA GTAACACACT CCAGAAACAC 
    AAATCTTCCT CCTCCTTTAC ACCTTTTATA GACCCCAGAT TACTACAGAT TTCTCCATCT 
    AGCGGGACAA CAGTGACTTC TGTGGTGGGG TTTTCCTGTG ATGGAATGAG ACCCGAGGCC 
    ATGAGGCAAG ATCCTACGCG GAAGGGCTCA GTGGTCAATG TCAATCCCAC TAACACTCGG 
    CCCCAGAGCG ACACGCCAGA GATTCGCAAA TACAAGAAGA GGTTTAACTC TGAGATCCTG 
    TGCGCTGCCT TGTGGGGAGT GAACTTGTTG GTGGGTACTG AGAGTGGCCT GATGCTGCTG 
    GACAGAAGTG GCCAAGGGAA GGTGTATCCT CTGATCAACA GAAGGCGATT TCAGCAGATG 
    GACGTCCTGG AAGGCTTGAA CGTCTTGGTG ACGATATCTG GGAAAAAGGA TAAGTTACGT 
    GTCTACTATT TGTCCTGGTT AAGAAATAAA ATACTTCACA ACGACCCAGA GGTTGAGAAG 
    AAGCAGGGCT GGACGACAGT GGGGGACCTG GAAGGCTGTG TGCATTATAA AGTTGTAAAA 
    TATGAAAGGA TCAAATTTCT GGTCATTGCT TTGAAGAACT CTGTGGAAGT CTACGCGTGG 
    GCACCCAAGC CATATCACAA ATTCATGGCC TTTAAGTCGT TTGGCGAGCT GGTGCACAAG 
    CCGTTGCTGG TGGATCTCAC TGTTGAGGAA GGCCAGAGGC TGAAAGTGAT CTATGGATCC 
    TGTGCTGGAT TCCATGCTGT TGATGTGGAC TCGGGATCGG TCTATGACAT TTACCTACCA 
    ACACACGTAA GAAAGAACCC ACACTCTATG ATCCAATGCA GCATCAAACC CCACGCGATC 
    ATCATCCTCC CCAACACAGA CGGCATGGAG CTCCTGGTGT GCTATGAAGA TGAAGGGGTT 
    TACGTAAACA CGTATGGGAG GATCACCAAG GATGTGGTTC TGCAGTGGGG AGAGATGCCG 
    ACGTCTGTAG CATATATTCG ATCCAATCAG ACGATGGGCT GGGGAGAAAA GGCCATAGAG 
    ATCCGATCTG TGGAAACTGG CCACTTGGAT GGCGTGTTTA TGCACAAAAG GGCTCAAAGG 
    CTGAAGTTCT TGTGTGAACG CAATGACAAG GTGTTCTTTG CCTCGGTCCG GTCTGGGGGC 
    AGCAGCCAGG TTTACTTCAT GACTCTCGGC AGGACCTCTC TTCTGAGCTG GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260744.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116529.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.
    Price & Availability↑
    CloneID OBu45798
    Clone ID Related Accession (Same CDS sequence) XM_025260744.1
    Accession Version XM_025260744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC 
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC 
    TCTCAGGACC CCTGCCCACC TTCCCGCAGT GAGGTGCTCA GTCAGAGTTC TGACTCTAAG 
    GCGGAGGTGC CCGACCCAGC CCAGAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTCC CTGTGAGAAC AACGTCACGG TCCCCCGTCC TGTCCCGCCG GGATTCCCCA 
    CTGCAGGGCA GTGGGCAGCA GAGTAACCAA GCTGGTCAGA GAAACTCCAC CAGTAGCATC 
    GAGCCAAGGC TGCTCTGGGA GAGAGTGGAG AAGCTGGCGC CCAGGCCAGG CAGTGGCAGC 
    TCCTCGGGGT CCAGCAACTC AGGGTCCCAG CCCGGCTCCC ACCCAGGGTC TCAGAGTGGC 
    TCCGGGGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCGCC ATCTCAGCGC 
    CTTGAAAACG CAGCAAAAAA GCCTGAAGAT AAAAAGGAAG TGTTTAGACC TCTCAAGCCT 
    GCTGGTGAAG TGGACCTGAC GGCGCTGGCC AAAGAGCTGC GGGCCGTGGA GGACGTGCGG 
    CCGCCGCACA AGGTGACGGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC GACGGACGAG 
    GAGGACGACG ACGCGGAGCA GGAAGGGGCC GAGGAGCCCA CGTCTGGCCC GGAGGACACC 
    AGAGCCGCAT CATCTCTGAA CTTGAGCAAC GGTGAAACGG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA GTGCAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCG 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCTT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCCGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACTAACAC TCGGCCCCAG 
    AGCGACACGC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCTGAGAT CCTGTGCGCT 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACTGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT GGTGACGATA TCTGGGAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CAGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC GTGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCGTTTGGCG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCGGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGCAGCATCA AACCCCACGC GATCATCATC 
    CTCCCCAACA CAGACGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTA 
    AACACGTATG GGAGGATCAC CAAGGATGTG GTTCTGCAGT GGGGAGAGAT GCCGACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCACA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCGG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116529.1
    CDS124..4011
    Translation

    Target ORF information:

    RefSeq Version XM_025260744.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGTG GTCCCACCTG GCATCTCTCA AGAACAATGT TTCCCCTGTC 
    TCGAGATCCC ATTCCTTCAG TGACCCTTCT CCTCCCAAAT TTGCACACCA CCATCTTCGC 
    TCTCAGGACC CCTGCCCACC TTCCCGCAGT GAGGTGCTCA GTCAGAGTTC TGACTCTAAG 
    GCGGAGGTGC CCGACCCAGC CCAGAAGGCT TGGTCTAGAT CAGACAGTGA CGAGGTGCCT 
    CCAAGGGTCC CTGTGAGAAC AACGTCACGG TCCCCCGTCC TGTCCCGCCG GGATTCCCCA 
    CTGCAGGGCA GTGGGCAGCA GAGTAACCAA GCTGGTCAGA GAAACTCCAC CAGTAGCATC 
    GAGCCAAGGC TGCTCTGGGA GAGAGTGGAG AAGCTGGCGC CCAGGCCAGG CAGTGGCAGC 
    TCCTCGGGGT CCAGCAACTC AGGGTCCCAG CCCGGCTCCC ACCCAGGGTC TCAGAGTGGC 
    TCCGGGGAGC GCTTCAGAGT GAGATCATCA TCCAAATCTG AAGGCTCGCC ATCTCAGCGC 
    CTTGAAAACG CAGCAAAAAA GCCTGAAGAT AAAAAGGAAG TGTTTAGACC TCTCAAGCCT 
    GCTGGTGAAG TGGACCTGAC GGCGCTGGCC AAAGAGCTGC GGGCCGTGGA GGACGTGCGG 
    CCGCCGCACA AGGTGACGGA CTACTCCTCC TCCAGCGAGG AGTCGGGGAC GACGGACGAG 
    GAGGACGACG ACGCGGAGCA GGAAGGGGCC GAGGAGCCCA CGTCTGGCCC GGAGGACACC 
    AGAGCCGCAT CATCTCTGAA CTTGAGCAAC GGTGAAACGG AGTCCGTGAA AACCATGGTT 
    GTCCATGACG ACGTGGAGAG TGAACCAGCC CTGACCCCGT CCAAAGAGGG CACGCTGATC 
    GTCCGCCAGA GTGCAGTTGA CCAAAAGCGC GCCAGCCATC ATGAGAGCAA TGGCTTTGCG 
    GGTCGCATTC ACCTCTTGCC AGATCTCTTA CAGCAAAGCC ATTCCTCCTC CACTTCCTCC 
    ACCTCCTCTT CCCCATCCTC CAGCCAGCCG ACACCCACCA TGTCCCCACA GACACCCCAG 
    GACAAGCTCA CTACTAATGA GACTCAGTCC GCTAGTAACA CACTCCAGAA ACACAAATCT 
    TCCTCCTCCT TTACACCTTT TATAGACCCC AGATTACTAC AGATTTCTCC ATCTAGCGGG 
    ACAACAGTGA CTTCTGTGGT GGGGTTTTCC TGTGATGGAA TGAGACCCGA GGCCATGAGG 
    CAAGATCCTA CGCGGAAGGG CTCAGTGGTC AATGTCAATC CCACTAACAC TCGGCCCCAG 
    AGCGACACGC CAGAGATTCG CAAATACAAG AAGAGGTTTA ACTCTGAGAT CCTGTGCGCT 
    GCCTTGTGGG GAGTGAACTT GTTGGTGGGT ACTGAGAGTG GCCTGATGCT GCTGGACAGA 
    AGTGGCCAAG GGAAGGTGTA TCCTCTGATC AACAGAAGGC GATTTCAGCA GATGGACGTC 
    CTGGAAGGCT TGAACGTCTT GGTGACGATA TCTGGGAAAA AGGATAAGTT ACGTGTCTAC 
    TATTTGTCCT GGTTAAGAAA TAAAATACTT CACAACGACC CAGAGGTTGA GAAGAAGCAG 
    GGCTGGACGA CAGTGGGGGA CCTGGAAGGC TGTGTGCATT ATAAAGTTGT AAAATATGAA 
    AGGATCAAAT TTCTGGTCAT TGCTTTGAAG AACTCTGTGG AAGTCTACGC GTGGGCACCC 
    AAGCCATATC ACAAATTCAT GGCCTTTAAG TCGTTTGGCG AGCTGGTGCA CAAGCCGTTG 
    CTGGTGGATC TCACTGTTGA GGAAGGCCAG AGGCTGAAAG TGATCTATGG ATCCTGTGCT 
    GGATTCCATG CTGTTGATGT GGACTCGGGA TCGGTCTATG ACATTTACCT ACCAACACAC 
    GTAAGAAAGA ACCCACACTC TATGATCCAA TGCAGCATCA AACCCCACGC GATCATCATC 
    CTCCCCAACA CAGACGGCAT GGAGCTCCTG GTGTGCTATG AAGATGAAGG GGTTTACGTA 
    AACACGTATG GGAGGATCAC CAAGGATGTG GTTCTGCAGT GGGGAGAGAT GCCGACGTCT 
    GTAGCATATA TTCGATCCAA TCAGACGATG GGCTGGGGAG AAAAGGCCAT AGAGATCCGA 
    TCTGTGGAAA CTGGCCACTT GGATGGCGTG TTTATGCACA AAAGGGCTCA AAGGCTGAAG 
    TTCTTGTGTG AACGCAATGA CAAGGTGTTC TTTGCCTCGG TCCGGTCTGG GGGCAGCAGC 
    CAGGTTTACT TCATGACTCT CGGCAGGACC TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260745.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116530.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.
    Price & Availability↑
    CloneID OBu45799
    Clone ID Related Accession (Same CDS sequence) XM_025260745.1
    Accession Version XM_025260745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCCGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC TAACACTCGG CCCCAGAGCG ACACGCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC TGAGATCCTG TGCGCTGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACTG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTGGTG 
    ACGATATCTG GGAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCAGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCGTGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCGT TTGGCGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCGGGATCGG TCTATGACAT TTACCTACCA ACACACATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116530.1
    CDS124..3963
    Translation

    Target ORF information:

    RefSeq Version XM_025260745.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTACAGTGGT CCCACCTGGC ATCTCTCAAG AACAATGTTT CCCCTGTCTC GAGATCCCAT 
    TCCTTCAGTG ACCCTTCTCC TCCCAAATTT GCACACCACC ATCTTCGCTC TCAGGACCCC 
    TGCCCACCTT CCCGCAGTGA GGTGCTCAGT CAGAGTTCTG ACTCTAAGGC GGAGGTGCCC 
    GACCCAGCCC AGAAGGCTTG GTCTAGATCA GACAGTGACG AGGTGCCTCC AAGGGTCCCT 
    GTGAGAACAA CGTCACGGTC CCCCGTCCTG TCCCGCCGGG ATTCCCCACT GCAGGGCAGT 
    GGGCAGCAGA GTAACCAAGC TGGTCAGAGA AACTCCACCA GTAGCATCGA GCCAAGGCTG 
    CTCTGGGAGA GAGTGGAGAA GCTGGCGCCC AGGCCAGGCA GTGGCAGCTC CTCGGGGTCC 
    AGCAACTCAG GGTCCCAGCC CGGCTCCCAC CCAGGGTCTC AGAGTGGCTC CGGGGAGCGC 
    TTCAGAGTGA GATCATCATC CAAATCTGAA GGCTCGCCAT CTCAGCGCCT TGAAAACGCA 
    GCAAAAAAGC CTGAAGATAA AAAGGAAGTG TTTAGACCTC TCAAGCCTGC TGGTGAAGTG 
    GACCTGACGG CGCTGGCCAA AGAGCTGCGG GCCGTGGAGG ACGTGCGGCC GCCGCACAAG 
    GTGACGGACT ACTCCTCCTC CAGCGAGGAG TCGGGGACGA CGGACGAGGA GGACGACGAC 
    GCGGAGCAGG AAGGGGCCGA GGAGCCCACG TCTGGCCCGG AGGACACCAG AGCCGCATCA 
    TCTCTGAACT TGAGCAACGG TGAAACGGAG TCCGTGAAAA CCATGGTTGT CCATGACGAC 
    GTGGAGAGTG AACCAGCCCT GACCCCGTCC AAAGAGGGCA CGCTGATCGT CCGCCAGACT 
    CAGTCCGCTA GTAACACACT CCAGAAACAC AAATCTTCCT CCTCCTTTAC ACCTTTTATA 
    GACCCCAGAT TACTACAGAT TTCTCCATCT AGCGGGACAA CAGTGACTTC TGTGGTGGGG 
    TTTTCCTGTG ATGGAATGAG ACCCGAGGCC ATGAGGCAAG ATCCTACGCG GAAGGGCTCA 
    GTGGTCAATG TCAATCCCAC TAACACTCGG CCCCAGAGCG ACACGCCAGA GATTCGCAAA 
    TACAAGAAGA GGTTTAACTC TGAGATCCTG TGCGCTGCCT TGTGGGGAGT GAACTTGTTG 
    GTGGGTACTG AGAGTGGCCT GATGCTGCTG GACAGAAGTG GCCAAGGGAA GGTGTATCCT 
    CTGATCAACA GAAGGCGATT TCAGCAGATG GACGTCCTGG AAGGCTTGAA CGTCTTGGTG 
    ACGATATCTG GGAAAAAGGA TAAGTTACGT GTCTACTATT TGTCCTGGTT AAGAAATAAA 
    ATACTTCACA ACGACCCAGA GGTTGAGAAG AAGCAGGGCT GGACGACAGT GGGGGACCTG 
    GAAGGCTGTG TGCATTATAA AGTTGTAAAA TATGAAAGGA TCAAATTTCT GGTCATTGCT 
    TTGAAGAACT CTGTGGAAGT CTACGCGTGG GCACCCAAGC CATATCACAA ATTCATGGCC 
    TTTAAGTCGT TTGGCGAGCT GGTGCACAAG CCGTTGCTGG TGGATCTCAC TGTTGAGGAA 
    GGCCAGAGGC TGAAAGTGAT CTATGGATCC TGTGCTGGAT TCCATGCTGT TGATGTGGAC 
    TCGGGATCGG TCTATGACAT TTACCTACCA ACACACATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260746.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116531.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.
    Price & Availability↑
    CloneID OBu45800
    Clone ID Related Accession (Same CDS sequence) XM_025260746.1
    Accession Version XM_025260746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CTGTTGCCCA GTTGCTGTGT TTATCACCAG GAAGCTCAGC 
    CAGTGCCCTT GCACAAGACA CTCGTATTCA GCCCGTGTGC AGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116531.1
    CDS127..3954
    Translation

    Target ORF information:

    RefSeq Version XM_025260746.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTACAGT GGTCCCACCT GGCATCTCTC 
    AAGAACAATG TTTCCCCTGT CTCGAGATCC CATTCCTTCA GTGACCCTTC TCCTCCCAAA 
    TTTGCACACC ACCATCTTCG CTCTCAGGAC CCCTGCCCAC CTTCCCGCAG TGAGGTGCTC 
    AGTCAGAGTT CTGACTCTAA GGCGGAGGTG CCCGACCCAG CCCAGAAGGC TTGGTCTAGA 
    TCAGACAGTG ACGAGGTGCC TCCAAGGGTC CCTGTGAGAA CAACGTCACG GTCCCCCGTC 
    CTGTCCCGCC GGGATTCCCC ACTGCAGGGC AGTGGGCAGC AGAGTAACCA AGCTGGTCAG 
    AGAAACTCCA CCAGTAGCAT CGAGCCAAGG CTGCTCTGGG AGAGAGTGGA GAAGCTGGCG 
    CCCAGGCCAG GCAGTGGCAG CTCCTCGGGG TCCAGCAACT CAGGGTCCCA GCCCGGCTCC 
    CACCCAGGGT CTCAGAGTGG CTCCGGGGAG CGCTTCAGAG TGAGATCATC ATCCAAATCT 
    GAAGGCTCGC CATCTCAGCG CCTTGAAAAC GCAGCAAAAA AGCCTGAAGA TAAAAAGGAA 
    GTGTTTAGAC CTCTCAAGCC TGCTGGTGAA GTGGACCTGA CGGCGCTGGC CAAAGAGCTG 
    CGGGCCGTGG AGGACGTGCG GCCGCCGCAC AAGGTGACGG ACTACTCCTC CTCCAGCGAG 
    GAGTCGGGGA CGACGGACGA GGAGGACGAC GACGCGGAGC AGGAAGGGGC CGAGGAGCCC 
    ACGTCTGGCC CGGAGGACAC CAGAGCCGCA TCATCTCTGA ACTTGAGCAA CGGTGAAACG 
    GAGTCCGTGA AAACCATGGT TGTCCATGAC GACGTGGAGA GTGAACCAGC CCTGACCCCG 
    TCCAAAGAGG GCACGCTGAT CGTCCGCCAG AGTGCAGTTG ACCAAAAGCG CGCCAGCCAT 
    CATGAGAGCA ATGGCTTTGC GGGTCGCATT CACCTCTTGC CAGATCTCTT ACAGCAAAGC 
    CATTCCTCCT CCACTTCCTC CACCTCCTCT TCCCCATCCT CCAGCCAGCC GACACCCACC 
    ATGTCCCCAC AGACACCCCA GGACAAGCTC ACTACTAATG AGACTCAGTC CGCTAGTAAC 
    ACACTCCAGA AACACAAATC TTCCTCCTCC TTTACACCTT TTATAGACCC CAGATTACTA 
    CAGATTTCTC CATCTAGCGG GACAACAGTG ACTTCTGTGG TGGGGTTTTC CTGTGATGGA 
    ATGAGACCCG AGGCCATGAG GCAAGATCCT ACGCGGAAGG GCTCAGTGGT CAATGTCAAT 
    CCCACTAACA CTCGGCCCCA GAGCGACACG CCAGAGATTC GCAAATACAA GAAGAGGTTT 
    AACTCTGAGA TCCTGTGCGC TGCCTTGTGG GGAGTGAACT TGTTGGTGGG TACTGAGAGT 
    GGCCTGATGC TGCTGGACAG AAGTGGCCAA GGGAAGGTGT ATCCTCTGAT CAACAGAAGG 
    CGATTTCAGC AGATGGACGT CCTGGAAGGC TTGAACGTCT TGGTGACGAT ATCTGGGAAA 
    AAGGATAAGT TACGTGTCTA CTATTTGTCC TGGTTAAGAA ATAAAATACT TCACAACGAC 
    CCAGAGGTTG AGAAGAAGCA GGGCTGGACG ACAGTGGGGG ACCTGGAAGG CTGTGTGCAT 
    TATAAAGTTG TAAAATATGA AAGGATCAAA TTTCTGGTCA TTGCTTTGAA GAACTCTGTG 
    GAAGTCTACG CGTGGGCACC CAAGCCATAT CACAAATTCA TGGCCTTTAA GTCGTTTGGC 
    GAGCTGGTGC ACAAGCCGTT GCTGGTGGAT CTCACTGTTG AGGAAGGCCA GAGGCTGAAA 
    GTGATCTATG GATCCTGTGC TGGATTCCAT GCTGTTGATG TGGACTCGGG ATCGGTCTAT 
    GACATTTACC TACCAACACA CTGTTGCCCA GTTGCTGTGT TTATCACCAG GAAGCTCAGC 
    CAGTGCCCTT GCACAAGACA CTCGTATTCA GCCCGTGTGC AGGAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260747.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116532.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.
    Price & Availability↑
    CloneID OBu45801
    Clone ID Related Accession (Same CDS sequence) XM_025260747.1
    Accession Version XM_025260747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCCGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACT AACACTCGGC CCCAGAGCGA CACGCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCT GAGATCCTGT GCGCTGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACTGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTGGTGA CGATATCTGG GAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCAGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCGTGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCGTT TGGCGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CGGGATCGGT CTATGACATT TACCTACCAA CACACATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116532.1
    CDS123..3911
    Translation

    Target ORF information:

    RefSeq Version XM_025260747.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCCGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACT AACACTCGGC CCCAGAGCGA CACGCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCT GAGATCCTGT GCGCTGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACTGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTGGTGA CGATATCTGG GAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCAGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCGTGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCGTT TGGCGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CGGGATCGGT CTATGACATT TACCTACCAA CACACATCCA ATGCAGCATC 
    AAACCCCACG CGATCATCAT CCTCCCCAAC ACAGACGGCA TGGAGCTCCT GGTGTGCTAT 
    GAAGATGAAG GGGTTTACGT AAACACGTAT GGGAGGATCA CCAAGGATGT GGTTCTGCAG 
    TGGGGAGAGA TGCCGACGTC TGTAGCATAT ATTCGATCCA ATCAGACGAT GGGCTGGGGA 
    GAAAAGGCCA TAGAGATCCG ATCTGTGGAA ACTGGCCACT TGGATGGCGT GTTTATGCAC 
    AAAAGGGCTC AAAGGCTGAA GTTCTTGTGT GAACGCAATG ACAAGGTGTT CTTTGCCTCG 
    GTCCGGTCTG GGGGCAGCAG CCAGGTTTAC TTCATGACTC TCGGCAGGAC CTCTCTTCTG 
    AGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260748.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116533.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.
    Price & Availability↑
    CloneID OBu45802
    Clone ID Related Accession (Same CDS sequence) XM_025260748.1
    Accession Version XM_025260748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTCCCTGTG 
    AGAACAACGT CACGGTCCCC CGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGCAGTGGG 
    CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC AAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCAGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCAGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGG TGAAGTGGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCCGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACT AACACTCGGC CCCAGAGCGA CACGCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCT GAGATCCTGT GCGCTGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACTGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTGGTGA CGATATCTGG GAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCAGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCGTGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCGTT TGGCGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CGGGATCGGT CTATGACATT TACCTACCAA CACACGTAAG AAAGAACCCA 
    CACTCTATGA TCCAATGCAG CATCAAACCC CACGCGATCA TCATCCTCCC CAACACAGAC 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTAAACAC GTATGGGAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCGA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCACAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGC 
    AATGACAAGG TGTTCTTTGC CTCGGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116533.1
    CDS124..3876
    Translation

    Target ORF information:

    RefSeq Version XM_025260748.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTCCCTGTG 
    AGAACAACGT CACGGTCCCC CGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGCAGTGGG 
    CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC TCCACCAGTA GCATCGAGCC AAGGCTGCTC 
    TGGGAGAGAG TGGAGAAGCT GGCGCCCAGG CCAGGCAGTG GCAGCTCCTC GGGGTCCAGC 
    AACTCAGGGT CCCAGCCCGG CTCCCACCCA GGGTCTCAGA GTGGCTCCGG GGAGCGCTTC 
    AGAGTGAGAT CATCATCCAA ATCTGAAGGC TCGCCATCTC AGCGCCTTGA AAACGCAGCA 
    AAAAAGCCTG AAGATAAAAA GGAAGTGTTT AGACCTCTCA AGCCTGCTGG TGAAGTGGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGAGTGCA 
    GTTGACCAAA AGCGCGCCAG CCATCATGAG AGCAATGGCT TTGCGGGTCG CATTCACCTC 
    TTGCCAGATC TCTTACAGCA AAGCCATTCC TCCTCCACTT CCTCCACCTC CTCTTCCCCA 
    TCCTCCAGCC AGCCGACACC CACCATGTCC CCACAGACAC CCCAGGACAA GCTCACTACT 
    AATGAGACTC AGTCCGCTAG TAACACACTC CAGAAACACA AATCTTCCTC CTCCTTTACA 
    CCTTTTATAG ACCCCAGATT ACTACAGATT TCTCCATCTA GCGGGACAAC AGTGACTTCT 
    GTGGTGGGGT TTTCCTGTGA TGGAATGAGA CCCGAGGCCA TGAGGCAAGA TCCTACGCGG 
    AAGGGCTCAG TGGTCAATGT CAATCCCACT AACACTCGGC CCCAGAGCGA CACGCCAGAG 
    ATTCGCAAAT ACAAGAAGAG GTTTAACTCT GAGATCCTGT GCGCTGCCTT GTGGGGAGTG 
    AACTTGTTGG TGGGTACTGA GAGTGGCCTG ATGCTGCTGG ACAGAAGTGG CCAAGGGAAG 
    GTGTATCCTC TGATCAACAG AAGGCGATTT CAGCAGATGG ACGTCCTGGA AGGCTTGAAC 
    GTCTTGGTGA CGATATCTGG GAAAAAGGAT AAGTTACGTG TCTACTATTT GTCCTGGTTA 
    AGAAATAAAA TACTTCACAA CGACCCAGAG GTTGAGAAGA AGCAGGGCTG GACGACAGTG 
    GGGGACCTGG AAGGCTGTGT GCATTATAAA GTTGTAAAAT ATGAAAGGAT CAAATTTCTG 
    GTCATTGCTT TGAAGAACTC TGTGGAAGTC TACGCGTGGG CACCCAAGCC ATATCACAAA 
    TTCATGGCCT TTAAGTCGTT TGGCGAGCTG GTGCACAAGC CGTTGCTGGT GGATCTCACT 
    GTTGAGGAAG GCCAGAGGCT GAAAGTGATC TATGGATCCT GTGCTGGATT CCATGCTGTT 
    GATGTGGACT CGGGATCGGT CTATGACATT TACCTACCAA CACACGTAAG AAAGAACCCA 
    CACTCTATGA TCCAATGCAG CATCAAACCC CACGCGATCA TCATCCTCCC CAACACAGAC 
    GGCATGGAGC TCCTGGTGTG CTATGAAGAT GAAGGGGTTT ACGTAAACAC GTATGGGAGG 
    ATCACCAAGG ATGTGGTTCT GCAGTGGGGA GAGATGCCGA CGTCTGTAGC ATATATTCGA 
    TCCAATCAGA CGATGGGCTG GGGAGAAAAG GCCATAGAGA TCCGATCTGT GGAAACTGGC 
    CACTTGGATG GCGTGTTTAT GCACAAAAGG GCTCAAAGGC TGAAGTTCTT GTGTGAACGC 
    AATGACAAGG TGTTCTTTGC CTCGGTCCGG TCTGGGGGCA GCAGCCAGGT TTACTTCATG 
    ACTCTCGGCA GGACCTCTCT TCTGAGCTGG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260749.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116534.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.
    Price & Availability↑
    CloneID OBu45803
    Clone ID Related Accession (Same CDS sequence) XM_025260749.1
    Accession Version XM_025260749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTCCCTGTG 
    AGAACAACGT CACGGTCCCC CGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGCAGTGGG 
    CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC TCCACCAGCA TCGAGCCAAG GCTGCTCTGG 
    GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC 
    TCAGGGTCCC AGCCCGGCTC CCACCCAGGG TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA 
    GTGAGATCAT CATCCAAATC TGAAGGCTCG CCATCTCAGC GCCTTGAAAA CGCAGCAAAA 
    AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA CCTCTCAAGC CTGCTGGTGA AGTGGACCTG 
    ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG GAGGACGTGC GGCCGCCGCA CAAGGTGACG 
    GACTACTCCT CCTCCAGCGA GGAGTCGGGG ACGACGGACG AGGAGGACGA CGACGCGGAG 
    CAGGAAGGGG CCGAGGAGCC CACGTCTGGC CCGGAGGACA CCAGAGCCGC ATCATCTCTG 
    AACTTGAGCA ACGGTGAAAC GGAGTCCGTG AAAACCATGG TTGTCCATGA CGACGTGGAG 
    AGTGAACCAG CCCTGACCCC GTCCAAAGAG GGCACGCTGA TCGTCCGCCA GAGTGCAGTT 
    GACCAAAAGC GCGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC TTCCCCATCC 
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTACTAAT 
    GAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCC GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACTAAC ACTCGGCCCC AGAGCGACAC GCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCTGAG ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACTGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTGGTGACGA TATCTGGGAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCAGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCGTGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCGG GATCGGTCTA TGACATTTAC CTACCAACAC ACGTAAGAAA GAACCCACAC 
    TCTATGATCC AATGCAGCAT CAAACCCCAC GCGATCATCA TCCTCCCCAA CACAGACGGC 
    ATGGAGCTCC TGGTGTGCTA TGAAGATGAA GGGGTTTACG TAAACACGTA TGGGAGGATC 
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAG ATGCCGACGT CTGTAGCATA TATTCGATCC 
    AATCAGACGA TGGGCTGGGG AGAAAAGGCC ATAGAGATCC GATCTGTGGA AACTGGCCAC 
    TTGGATGGCG TGTTTATGCA CAAAAGGGCT CAAAGGCTGA AGTTCTTGTG TGAACGCAAT 
    GACAAGGTGT TCTTTGCCTC GGTCCGGTCT GGGGGCAGCA GCCAGGTTTA CTTCATGACT 
    CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116534.1
    CDS124..3873
    Translation

    Target ORF information:

    RefSeq Version XM_025260749.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTCCCTGTG 
    AGAACAACGT CACGGTCCCC CGTCCTGTCC CGCCGGGATT CCCCACTGCA GGGCAGTGGG 
    CAGCAGAGTA ACCAAGCTGG TCAGAGAAAC TCCACCAGCA TCGAGCCAAG GCTGCTCTGG 
    GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC 
    TCAGGGTCCC AGCCCGGCTC CCACCCAGGG TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA 
    GTGAGATCAT CATCCAAATC TGAAGGCTCG CCATCTCAGC GCCTTGAAAA CGCAGCAAAA 
    AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA CCTCTCAAGC CTGCTGGTGA AGTGGACCTG 
    ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG GAGGACGTGC GGCCGCCGCA CAAGGTGACG 
    GACTACTCCT CCTCCAGCGA GGAGTCGGGG ACGACGGACG AGGAGGACGA CGACGCGGAG 
    CAGGAAGGGG CCGAGGAGCC CACGTCTGGC CCGGAGGACA CCAGAGCCGC ATCATCTCTG 
    AACTTGAGCA ACGGTGAAAC GGAGTCCGTG AAAACCATGG TTGTCCATGA CGACGTGGAG 
    AGTGAACCAG CCCTGACCCC GTCCAAAGAG GGCACGCTGA TCGTCCGCCA GAGTGCAGTT 
    GACCAAAAGC GCGCCAGCCA TCATGAGAGC AATGGCTTTG CGGGTCGCAT TCACCTCTTG 
    CCAGATCTCT TACAGCAAAG CCATTCCTCC TCCACTTCCT CCACCTCCTC TTCCCCATCC 
    TCCAGCCAGC CGACACCCAC CATGTCCCCA CAGACACCCC AGGACAAGCT CACTACTAAT 
    GAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCC GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACTAAC ACTCGGCCCC AGAGCGACAC GCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCTGAG ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACTGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTGGTGACGA TATCTGGGAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCAGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCGTGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCGG GATCGGTCTA TGACATTTAC CTACCAACAC ACGTAAGAAA GAACCCACAC 
    TCTATGATCC AATGCAGCAT CAAACCCCAC GCGATCATCA TCCTCCCCAA CACAGACGGC 
    ATGGAGCTCC TGGTGTGCTA TGAAGATGAA GGGGTTTACG TAAACACGTA TGGGAGGATC 
    ACCAAGGATG TGGTTCTGCA GTGGGGAGAG ATGCCGACGT CTGTAGCATA TATTCGATCC 
    AATCAGACGA TGGGCTGGGG AGAAAAGGCC ATAGAGATCC GATCTGTGGA AACTGGCCAC 
    TTGGATGGCG TGTTTATGCA CAAAAGGGCT CAAAGGCTGA AGTTCTTGTG TGAACGCAAT 
    GACAAGGTGT TCTTTGCCTC GGTCCGGTCT GGGGGCAGCA GCCAGGTTTA CTTCATGACT 
    CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260750.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116535.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.
    Price & Availability↑
    CloneID OBu45804
    Clone ID Related Accession (Same CDS sequence) XM_025260750.1
    Accession Version XM_025260750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCA AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGAC CTGACGGCGC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGACG TGCGGCCGCC GCACAAGGTG ACGGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACGACGG ACGAGGAGGA CGACGACGCG GAGCAGGAAG GGGCCGAGGA GCCCACGTCT 
    GGCCCGGAGG ACACCAGAGC CGCATCATCT CTGAACTTGA GCAACGGTGA AACGGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGACTCAG TCCGCTAGTA ACACACTCCA GAAACACAAA 
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
    GGGACAACAG TGACTTCTGT GGTGGGGTTT TCCTGTGATG GAATGAGACC CGAGGCCATG 
    AGGCAAGATC CTACGCGGAA GGGCTCAGTG GTCAATGTCA ATCCCACTAA CACTCGGCCC 
    CAGAGCGACA CGCCAGAGAT TCGCAAATAC AAGAAGAGGT TTAACTCTGA GATCCTGTGC 
    GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACTGAGA GTGGCCTGAT GCTGCTGGAC 
    AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GGCGATTTCA GCAGATGGAC 
    GTCCTGGAAG GCTTGAACGT CTTGGTGACG ATATCTGGGA AAAAGGATAA GTTACGTGTC 
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ACCCAGAGGT TGAGAAGAAG 
    CAGGGCTGGA CGACAGTGGG GGACCTGGAA GGCTGTGTGC ATTATAAAGT TGTAAAATAT 
    GAAAGGATCA AATTTCTGGT CATTGCTTTG AAGAACTCTG TGGAAGTCTA CGCGTGGGCA 
    CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCGTTTG GCGAGCTGGT GCACAAGCCG 
    TTGCTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGCTGA AAGTGATCTA TGGATCCTGT 
    GCTGGATTCC ATGCTGTTGA TGTGGACTCG GGATCGGTCT ATGACATTTA CCTACCAACA 
    CACATCCAAT GCAGCATCAA ACCCCACGCG ATCATCATCC TCCCCAACAC AGACGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTAA ACACGTATGG GAGGATCACC 
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCGACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCACAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGCAATGAC 
    AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116535.1
    CDS123..3809
    Translation

    Target ORF information:

    RefSeq Version XM_025260750.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGGAGTGCCG CTGGCGGGAG 
    CTGGAGGAGC ACCGGCAGGC AGAGCGGCTC CAGAGGCAGC TGCAGCAAGA ACAAGCCTAT 
    CTCCTGTCTC TACAGCACGA CCCCCGGAGG CCGCCCCCGC CGCCAGACAG GAGCAAGGCT 
    GGCCTGCACG AGCCCAAGGC CCCGCACGAG CCCGCTGACC GGGCCCGCGA GGTGGAAGAT 
    AGATTTAGGA AAACTAACCA CAGCTCCCCC GAAGCCCAGT CTAAGCAGAC GGGCCGAGTA 
    TTGGAACCTC CAGTGCCTTC CAGATCAGAG TCTTTTTCCA GTGGCAGCTC CGAGTCTGTG 
    CACCCTGCCC TGCAGAGACC CGCCGAGCCA CAGGTCCCTG TGAGAACAAC GTCACGGTCC 
    CCCGTCCTGT CCCGCCGGGA TTCCCCACTG CAGGGCAGTG GGCAGCAGAG TAACCAAGCT 
    GGTCAGAGAA ACTCCACCAG CATCGAGCCA AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG 
    GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC 
    TCCCACCCAG GGTCTCAGAG TGGCTCCGGG GAGCGCTTCA GAGTGAGATC ATCATCCAAA 
    TCTGAAGGCT CGCCATCTCA GCGCCTTGAA AACGCAGCAA AAAAGCCTGA AGATAAAAAG 
    GAAGTGTTTA GACCTCTCAA GCCTGCTGAC CTGACGGCGC TGGCCAAAGA GCTGCGGGCC 
    GTGGAGGACG TGCGGCCGCC GCACAAGGTG ACGGACTACT CCTCCTCCAG CGAGGAGTCG 
    GGGACGACGG ACGAGGAGGA CGACGACGCG GAGCAGGAAG GGGCCGAGGA GCCCACGTCT 
    GGCCCGGAGG ACACCAGAGC CGCATCATCT CTGAACTTGA GCAACGGTGA AACGGAGTCC 
    GTGAAAACCA TGGTTGTCCA TGACGACGTG GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA 
    GAGGGCACGC TGATCGTCCG CCAGACTCAG TCCGCTAGTA ACACACTCCA GAAACACAAA 
    TCTTCCTCCT CCTTTACACC TTTTATAGAC CCCAGATTAC TACAGATTTC TCCATCTAGC 
    GGGACAACAG TGACTTCTGT GGTGGGGTTT TCCTGTGATG GAATGAGACC CGAGGCCATG 
    AGGCAAGATC CTACGCGGAA GGGCTCAGTG GTCAATGTCA ATCCCACTAA CACTCGGCCC 
    CAGAGCGACA CGCCAGAGAT TCGCAAATAC AAGAAGAGGT TTAACTCTGA GATCCTGTGC 
    GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG GGTACTGAGA GTGGCCTGAT GCTGCTGGAC 
    AGAAGTGGCC AAGGGAAGGT GTATCCTCTG ATCAACAGAA GGCGATTTCA GCAGATGGAC 
    GTCCTGGAAG GCTTGAACGT CTTGGTGACG ATATCTGGGA AAAAGGATAA GTTACGTGTC 
    TACTATTTGT CCTGGTTAAG AAATAAAATA CTTCACAACG ACCCAGAGGT TGAGAAGAAG 
    CAGGGCTGGA CGACAGTGGG GGACCTGGAA GGCTGTGTGC ATTATAAAGT TGTAAAATAT 
    GAAAGGATCA AATTTCTGGT CATTGCTTTG AAGAACTCTG TGGAAGTCTA CGCGTGGGCA 
    CCCAAGCCAT ATCACAAATT CATGGCCTTT AAGTCGTTTG GCGAGCTGGT GCACAAGCCG 
    TTGCTGGTGG ATCTCACTGT TGAGGAAGGC CAGAGGCTGA AAGTGATCTA TGGATCCTGT 
    GCTGGATTCC ATGCTGTTGA TGTGGACTCG GGATCGGTCT ATGACATTTA CCTACCAACA 
    CACATCCAAT GCAGCATCAA ACCCCACGCG ATCATCATCC TCCCCAACAC AGACGGCATG 
    GAGCTCCTGG TGTGCTATGA AGATGAAGGG GTTTACGTAA ACACGTATGG GAGGATCACC 
    AAGGATGTGG TTCTGCAGTG GGGAGAGATG CCGACGTCTG TAGCATATAT TCGATCCAAT 
    CAGACGATGG GCTGGGGAGA AAAGGCCATA GAGATCCGAT CTGTGGAAAC TGGCCACTTG 
    GATGGCGTGT TTATGCACAA AAGGGCTCAA AGGCTGAAGT TCTTGTGTGA ACGCAATGAC 
    AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG GGCAGCAGCC AGGTTTACTT CATGACTCTC 
    GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260751.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116536.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.
    Price & Availability↑
    CloneID OBu45805
    Clone ID Related Accession (Same CDS sequence) XM_025260751.1
    Accession Version XM_025260751.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAA GGGTCCCTGT GAGAACAACG TCACGGTCCC CCGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGCAGTGG GCAGCAGAGT AACCAAGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CAAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCAGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCG CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC 
    GTGCGGCCGC CGCACAAGGT GACGGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACGACG 
    GACGAGGAGG ACGACGACGC GGAGCAGGAA GGGGCCGAGG AGCCCACGTC TGGCCCGGAG 
    GACACCAGAG CCGCATCATC TCTGAACTTG AGCAACGGTG AAACGGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGGACAACA 
    GTGACTTCTG TGGTGGGGTT TTCCTGTGAT GGAATGAGAC CCGAGGCCAT GAGGCAAGAT 
    CCTACGCGGA AGGGCTCAGT GGTCAATGTC AATCCCACTA ACACTCGGCC CCAGAGCGAC 
    ACGCCAGAGA TTCGCAAATA CAAGAAGAGG TTTAACTCTG AGATCCTGTG CGCTGCCTTG 
    TGGGGAGTGA ACTTGTTGGT GGGTACTGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
    CAAGGGAAGG TGTATCCTCT GATCAACAGA AGGCGATTTC AGCAGATGGA CGTCCTGGAA 
    GGCTTGAACG TCTTGGTGAC GATATCTGGG AAAAAGGATA AGTTACGTGT CTACTATTTG 
    TCCTGGTTAA GAAATAAAAT ACTTCACAAC GACCCAGAGG TTGAGAAGAA GCAGGGCTGG 
    ACGACAGTGG GGGACCTGGA AGGCTGTGTG CATTATAAAG TTGTAAAATA TGAAAGGATC 
    AAATTTCTGG TCATTGCTTT GAAGAACTCT GTGGAAGTCT ACGCGTGGGC ACCCAAGCCA 
    TATCACAAAT TCATGGCCTT TAAGTCGTTT GGCGAGCTGG TGCACAAGCC GTTGCTGGTG 
    GATCTCACTG TTGAGGAAGG CCAGAGGCTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
    CATGCTGTTG ATGTGGACTC GGGATCGGTC TATGACATTT ACCTACCAAC ACACATCCAA 
    TGCAGCATCA AACCCCACGC GATCATCATC CTCCCCAACA CAGACGGCAT GGAGCTCCTG 
    GTGTGCTATG AAGATGAAGG GGTTTACGTA AACACGTATG GGAGGATCAC CAAGGATGTG 
    GTTCTGCAGT GGGGAGAGAT GCCGACGTCT GTAGCATATA TTCGATCCAA TCAGACGATG 
    GGCTGGGGAG AAAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGCCACTT GGATGGCGTG 
    TTTATGCACA AAAGGGCTCA AAGGCTGAAG TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
    TTTGCCTCGG TCCGGTCTGG GGGCAGCAGC CAGGTTTACT TCATGACTCT CGGCAGGACC 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116536.1
    CDS123..3800
    Translation

    Target ORF information:

    RefSeq Version XM_025260751.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260751.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT ACAGTGGTCC CACCTGGCAT CTCTCAAGAA CAATGTTTCC 
    CCTGTCTCGA GATCCCATTC CTTCAGTGAC CCTTCTCCTC CCAAATTTGC ACACCACCAT 
    CTTCGCTCTC AGGACCCCTG CCCACCTTCC CGCAGTGAGG TGCTCAGTCA GAGTTCTGAC 
    TCTAAGGCGG AGGTGCCCGA CCCAGCCCAG AAGGCTTGGT CTAGATCAGA CAGTGACGAG 
    GTGCCTCCAA GGGTCCCTGT GAGAACAACG TCACGGTCCC CCGTCCTGTC CCGCCGGGAT 
    TCCCCACTGC AGGGCAGTGG GCAGCAGAGT AACCAAGCTG GTCAGAGAAA CTCCACCAGT 
    AGCATCGAGC CAAGGCTGCT CTGGGAGAGA GTGGAGAAGC TGGCGCCCAG GCCAGGCAGT 
    GGCAGCTCCT CGGGGTCCAG CAACTCAGGG TCCCAGCCCG GCTCCCACCC AGGGTCTCAG 
    AGTGGCTCCG GGGAGCGCTT CAGAGTGAGA TCATCATCCA AATCTGAAGG CTCGCCATCT 
    CAGCGCCTTG AAAACGCAGC AAAAAAGCCT GAAGATAAAA AGGAAGTGTT TAGACCTCTC 
    AAGCCTGCTG GTGAAGTGGA CCTGACGGCG CTGGCCAAAG AGCTGCGGGC CGTGGAGGAC 
    GTGCGGCCGC CGCACAAGGT GACGGACTAC TCCTCCTCCA GCGAGGAGTC GGGGACGACG 
    GACGAGGAGG ACGACGACGC GGAGCAGGAA GGGGCCGAGG AGCCCACGTC TGGCCCGGAG 
    GACACCAGAG CCGCATCATC TCTGAACTTG AGCAACGGTG AAACGGAGTC CGTGAAAACC 
    ATGGTTGTCC ATGACGACGT GGAGAGTGAA CCAGCCCTGA CCCCGTCCAA AGAGGGCACG 
    CTGATCGTCC GCCAGACTCA GTCCGCTAGT AACACACTCC AGAAACACAA ATCTTCCTCC 
    TCCTTTACAC CTTTTATAGA CCCCAGATTA CTACAGATTT CTCCATCTAG CGGGACAACA 
    GTGACTTCTG TGGTGGGGTT TTCCTGTGAT GGAATGAGAC CCGAGGCCAT GAGGCAAGAT 
    CCTACGCGGA AGGGCTCAGT GGTCAATGTC AATCCCACTA ACACTCGGCC CCAGAGCGAC 
    ACGCCAGAGA TTCGCAAATA CAAGAAGAGG TTTAACTCTG AGATCCTGTG CGCTGCCTTG 
    TGGGGAGTGA ACTTGTTGGT GGGTACTGAG AGTGGCCTGA TGCTGCTGGA CAGAAGTGGC 
    CAAGGGAAGG TGTATCCTCT GATCAACAGA AGGCGATTTC AGCAGATGGA CGTCCTGGAA 
    GGCTTGAACG TCTTGGTGAC GATATCTGGG AAAAAGGATA AGTTACGTGT CTACTATTTG 
    TCCTGGTTAA GAAATAAAAT ACTTCACAAC GACCCAGAGG TTGAGAAGAA GCAGGGCTGG 
    ACGACAGTGG GGGACCTGGA AGGCTGTGTG CATTATAAAG TTGTAAAATA TGAAAGGATC 
    AAATTTCTGG TCATTGCTTT GAAGAACTCT GTGGAAGTCT ACGCGTGGGC ACCCAAGCCA 
    TATCACAAAT TCATGGCCTT TAAGTCGTTT GGCGAGCTGG TGCACAAGCC GTTGCTGGTG 
    GATCTCACTG TTGAGGAAGG CCAGAGGCTG AAAGTGATCT ATGGATCCTG TGCTGGATTC 
    CATGCTGTTG ATGTGGACTC GGGATCGGTC TATGACATTT ACCTACCAAC ACACATCCAA 
    TGCAGCATCA AACCCCACGC GATCATCATC CTCCCCAACA CAGACGGCAT GGAGCTCCTG 
    GTGTGCTATG AAGATGAAGG GGTTTACGTA AACACGTATG GGAGGATCAC CAAGGATGTG 
    GTTCTGCAGT GGGGAGAGAT GCCGACGTCT GTAGCATATA TTCGATCCAA TCAGACGATG 
    GGCTGGGGAG AAAAGGCCAT AGAGATCCGA TCTGTGGAAA CTGGCCACTT GGATGGCGTG 
    TTTATGCACA AAAGGGCTCA AAGGCTGAAG TTCTTGTGTG AACGCAATGA CAAGGTGTTC 
    TTTGCCTCGG TCCGGTCTGG GGGCAGCAGC CAGGTTTACT TCATGACTCT CGGCAGGACC 
    TCTCTTCTGA GCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260752.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116537.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.
    Price & Availability↑
    CloneID OBu45806
    Clone ID Related Accession (Same CDS sequence) XM_025260752.1
    Accession Version XM_025260752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCAAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116537.1
    CDS124..3783
    Translation

    Target ORF information:

    RefSeq Version XM_025260752.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCAAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACGTAAGAAA GAACCCACAC TCTATGATCC AATGCAGCAT CAAACCCCAC 
    GCGATCATCA TCCTCCCCAA CACAGACGGC ATGGAGCTCC TGGTGTGCTA TGAAGATGAA 
    GGGGTTTACG TAAACACGTA TGGGAGGATC ACCAAGGATG TGGTTCTGCA GTGGGGAGAG 
    ATGCCGACGT CTGTAGCATA TATTCGATCC AATCAGACGA TGGGCTGGGG AGAAAAGGCC 
    ATAGAGATCC GATCTGTGGA AACTGGCCAC TTGGATGGCG TGTTTATGCA CAAAAGGGCT 
    CAAAGGCTGA AGTTCTTGTG TGAACGCAAT GACAAGGTGT TCTTTGCCTC GGTCCGGTCT 
    GGGGGCAGCA GCCAGGTTTA CTTCATGACT CTCGGCAGGA CCTCTCTTCT GAGCTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260753.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116538.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.
    Price & Availability↑
    CloneID OBu45807
    Clone ID Related Accession (Same CDS sequence) XM_025260753.1
    Accession Version XM_025260753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3636bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCAAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116538.1
    CDS122..3757
    Translation

    Target ORF information:

    RefSeq Version XM_025260753.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGTAGCA TCGAGCCAAG GCTGCTCTGG GAGAGAGTGG AGAAGCTGGC GCCCAGGCCA 
    GGCAGTGGCA GCTCCTCGGG GTCCAGCAAC TCAGGGTCCC AGCCCGGCTC CCACCCAGGG 
    TCTCAGAGTG GCTCCGGGGA GCGCTTCAGA GTGAGATCAT CATCCAAATC TGAAGGCTCG 
    CCATCTCAGC GCCTTGAAAA CGCAGCAAAA AAGCCTGAAG ATAAAAAGGA AGTGTTTAGA 
    CCTCTCAAGC CTGCTGGTGA AGTGGACCTG ACGGCGCTGG CCAAAGAGCT GCGGGCCGTG 
    GAGGACGTGC GGCCGCCGCA CAAGGTGACG GACTACTCCT CCTCCAGCGA GGAGTCGGGG 
    ACGACGGACG AGGAGGACGA CGACGCGGAG CAGGAAGGGG CCGAGGAGCC CACGTCTGGC 
    CCGGAGGACA CCAGAGCCGC ATCATCTCTG AACTTGAGCA ACGGTGAAAC GGAGTCCGTG 
    AAAACCATGG TTGTCCATGA CGACGTGGAG AGTGAACCAG CCCTGACCCC GTCCAAAGAG 
    GGCACGCTGA TCGTCCGCCA GAGTGCAGTT GACCAAAAGC GCGCCAGCCA TCATGAGAGC 
    AATGGCTTTG CGGGTCGCAT TCACCTCTTG CCAGATCTCT TACAGCAAAG CCATTCCTCC 
    TCCACTTCCT CCACCTCCTC TTCCCCATCC TCCAGCCAGC CGACACCCAC CATGTCCCCA 
    CAGACACCCC AGGACAAGCT CACTACTAAT GAGACTCAGT CCGCTAGTAA CACACTCCAG 
    AAACACAAAT CTTCCTCCTC CTTTACACCT TTTATAGACC CCAGATTACT ACAGATTTCT 
    CCATCTAGCG GGACAACAGT GACTTCTGTG GTGGGGTTTT CCTGTGATGG AATGAGACCC 
    GAGGCCATGA GGCAAGATCC TACGCGGAAG GGCTCAGTGG TCAATGTCAA TCCCACTAAC 
    ACTCGGCCCC AGAGCGACAC GCCAGAGATT CGCAAATACA AGAAGAGGTT TAACTCTGAG 
    ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC TTGTTGGTGG GTACTGAGAG TGGCCTGATG 
    CTGCTGGACA GAAGTGGCCA AGGGAAGGTG TATCCTCTGA TCAACAGAAG GCGATTTCAG 
    CAGATGGACG TCCTGGAAGG CTTGAACGTC TTGGTGACGA TATCTGGGAA AAAGGATAAG 
    TTACGTGTCT ACTATTTGTC CTGGTTAAGA AATAAAATAC TTCACAACGA CCCAGAGGTT 
    GAGAAGAAGC AGGGCTGGAC GACAGTGGGG GACCTGGAAG GCTGTGTGCA TTATAAAGTT 
    GTAAAATATG AAAGGATCAA ATTTCTGGTC ATTGCTTTGA AGAACTCTGT GGAAGTCTAC 
    GCGTGGGCAC CCAAGCCATA TCACAAATTC ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG 
    CACAAGCCGT TGCTGGTGGA TCTCACTGTT GAGGAAGGCC AGAGGCTGAA AGTGATCTAT 
    GGATCCTGTG CTGGATTCCA TGCTGTTGAT GTGGACTCGG GATCGGTCTA TGACATTTAC 
    CTACCAACAC ACATCCAATG CAGCATCAAA CCCCACGCGA TCATCATCCT CCCCAACACA 
    GACGGCATGG AGCTCCTGGT GTGCTATGAA GATGAAGGGG TTTACGTAAA CACGTATGGG 
    AGGATCACCA AGGATGTGGT TCTGCAGTGG GGAGAGATGC CGACGTCTGT AGCATATATT 
    CGATCCAATC AGACGATGGG CTGGGGAGAA AAGGCCATAG AGATCCGATC TGTGGAAACT 
    GGCCACTTGG ATGGCGTGTT TATGCACAAA AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA 
    CGCAATGACA AGGTGTTCTT TGCCTCGGTC CGGTCTGGGG GCAGCAGCCA GGTTTACTTC 
    ATGACTCTCG GCAGGACCTC TCTTCTGAGC TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260754.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116539.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.
    Price & Availability↑
    CloneID OBu45808
    Clone ID Related Accession (Same CDS sequence) XM_025260754.1
    Accession Version XM_025260754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGGT 
    GAAGTGGACC TGACGGCGCT GGCCAAAGAG CTGCGGGCCG TGGAGGACGT GCGGCCGCCG 
    CACAAGGTGA CGGACTACTC CTCCTCCAGC GAGGAGTCGG GGACGACGGA CGAGGAGGAC 
    GACGACGCGG AGCAGGAAGG GGCCGAGGAG CCCACGTCTG GCCCGGAGGA CACCAGAGCC 
    GCATCATCTC TGAACTTGAG CAACGGTGAA ACGGAGTCCG TGAAAACCAT GGTTGTCCAT 
    GACGACGTGG AGAGTGAACC AGCCCTGACC CCGTCCAAAG AGGGCACGCT GATCGTCCGC 
    CAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCC GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACTAAC ACTCGGCCCC AGAGCGACAC GCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCTGAG ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACTGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTGGTGACGA TATCTGGGAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCAGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCGTGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCGG GATCGGTCTA TGACATTTAC CTACCAACAC ACATCCAATG CAGCATCAAA 
    CCCCACGCGA TCATCATCCT CCCCAACACA GACGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTAAA CACGTATGGG AGGATCACCA AGGATGTGGT TCTGCAGTGG 
    GGAGAGATGC CGACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCACAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCGGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116539.1
    CDS123..3728
    Translation

    Target ORF information:

    RefSeq Version XM_025260754.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGGT 
    GAAGTGGACC TGACGGCGCT GGCCAAAGAG CTGCGGGCCG TGGAGGACGT GCGGCCGCCG 
    CACAAGGTGA CGGACTACTC CTCCTCCAGC GAGGAGTCGG GGACGACGGA CGAGGAGGAC 
    GACGACGCGG AGCAGGAAGG GGCCGAGGAG CCCACGTCTG GCCCGGAGGA CACCAGAGCC 
    GCATCATCTC TGAACTTGAG CAACGGTGAA ACGGAGTCCG TGAAAACCAT GGTTGTCCAT 
    GACGACGTGG AGAGTGAACC AGCCCTGACC CCGTCCAAAG AGGGCACGCT GATCGTCCGC 
    CAGACTCAGT CCGCTAGTAA CACACTCCAG AAACACAAAT CTTCCTCCTC CTTTACACCT 
    TTTATAGACC CCAGATTACT ACAGATTTCT CCATCTAGCG GGACAACAGT GACTTCTGTG 
    GTGGGGTTTT CCTGTGATGG AATGAGACCC GAGGCCATGA GGCAAGATCC TACGCGGAAG 
    GGCTCAGTGG TCAATGTCAA TCCCACTAAC ACTCGGCCCC AGAGCGACAC GCCAGAGATT 
    CGCAAATACA AGAAGAGGTT TAACTCTGAG ATCCTGTGCG CTGCCTTGTG GGGAGTGAAC 
    TTGTTGGTGG GTACTGAGAG TGGCCTGATG CTGCTGGACA GAAGTGGCCA AGGGAAGGTG 
    TATCCTCTGA TCAACAGAAG GCGATTTCAG CAGATGGACG TCCTGGAAGG CTTGAACGTC 
    TTGGTGACGA TATCTGGGAA AAAGGATAAG TTACGTGTCT ACTATTTGTC CTGGTTAAGA 
    AATAAAATAC TTCACAACGA CCCAGAGGTT GAGAAGAAGC AGGGCTGGAC GACAGTGGGG 
    GACCTGGAAG GCTGTGTGCA TTATAAAGTT GTAAAATATG AAAGGATCAA ATTTCTGGTC 
    ATTGCTTTGA AGAACTCTGT GGAAGTCTAC GCGTGGGCAC CCAAGCCATA TCACAAATTC 
    ATGGCCTTTA AGTCGTTTGG CGAGCTGGTG CACAAGCCGT TGCTGGTGGA TCTCACTGTT 
    GAGGAAGGCC AGAGGCTGAA AGTGATCTAT GGATCCTGTG CTGGATTCCA TGCTGTTGAT 
    GTGGACTCGG GATCGGTCTA TGACATTTAC CTACCAACAC ACATCCAATG CAGCATCAAA 
    CCCCACGCGA TCATCATCCT CCCCAACACA GACGGCATGG AGCTCCTGGT GTGCTATGAA 
    GATGAAGGGG TTTACGTAAA CACGTATGGG AGGATCACCA AGGATGTGGT TCTGCAGTGG 
    GGAGAGATGC CGACGTCTGT AGCATATATT CGATCCAATC AGACGATGGG CTGGGGAGAA 
    AAGGCCATAG AGATCCGATC TGTGGAAACT GGCCACTTGG ATGGCGTGTT TATGCACAAA 
    AGGGCTCAAA GGCTGAAGTT CTTGTGTGAA CGCAATGACA AGGTGTTCTT TGCCTCGGTC 
    CGGTCTGGGG GCAGCAGCCA GGTTTACTTC ATGACTCTCG GCAGGACCTC TCTTCTGAGC 
    TGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260755.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116540.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.
    Price & Availability↑
    CloneID OBu45809
    Clone ID Related Accession (Same CDS sequence) XM_025260755.1
    Accession Version XM_025260755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3597bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC CGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACTAA CACTCGGCCC CAGAGCGACA CGCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCTGA GATCCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACTGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTGGTGACG 
    ATATCTGGGA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCAGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCGTTTG GCGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCG 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACATCCAAT GCAGCATCAA ACCCCACGCG 
    ATCATCATCC TCCCCAACAC AGACGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTAA ACACGTATGG GAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
    CCGACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCACAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116540.1
    CDS123..3719
    Translation

    Target ORF information:

    RefSeq Version XM_025260755.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT GGAAGATAGA TTTAGGAAAA CTAACCACAG CTCCCCCGAA 
    GCCCAGTCTA AGCAGACGGG CCGAGTATTG GAACCTCCAG TGCCTTCCAG ATCAGAGTCT 
    TTTTCCAGTG GCAGCTCCGA GTCTGTGCAC CCTGCCCTGC AGAGACCCGC CGAGCCACAG 
    GTCCCTGTGA GAACAACGTC ACGGTCCCCC GTCCTGTCCC GCCGGGATTC CCCACTGCAG 
    GGCAGTGGGC AGCAGAGTAA CCAAGCTGGT CAGAGAAACT CCACCAGTAG CATCGAGCCA 
    AGGCTGCTCT GGGAGAGAGT GGAGAAGCTG GCGCCCAGGC CAGGCAGTGG CAGCTCCTCG 
    GGGTCCAGCA ACTCAGGGTC CCAGCCCGGC TCCCACCCAG GGTCTCAGAG TGGCTCCGGG 
    GAGCGCTTCA GAGTGAGATC ATCATCCAAA TCTGAAGGCT CGCCATCTCA GCGCCTTGAA 
    AACGCAGCAA AAAAGCCTGA AGATAAAAAG GAAGTGTTTA GACCTCTCAA GCCTGCTGAC 
    CTGACGGCGC TGGCCAAAGA GCTGCGGGCC GTGGAGGACG TGCGGCCGCC GCACAAGGTG 
    ACGGACTACT CCTCCTCCAG CGAGGAGTCG GGGACGACGG ACGAGGAGGA CGACGACGCG 
    GAGCAGGAAG GGGCCGAGGA GCCCACGTCT GGCCCGGAGG ACACCAGAGC CGCATCATCT 
    CTGAACTTGA GCAACGGTGA AACGGAGTCC GTGAAAACCA TGGTTGTCCA TGACGACGTG 
    GAGAGTGAAC CAGCCCTGAC CCCGTCCAAA GAGGGCACGC TGATCGTCCG CCAGACTCAG 
    TCCGCTAGTA ACACACTCCA GAAACACAAA TCTTCCTCCT CCTTTACACC TTTTATAGAC 
    CCCAGATTAC TACAGATTTC TCCATCTAGC GGGACAACAG TGACTTCTGT GGTGGGGTTT 
    TCCTGTGATG GAATGAGACC CGAGGCCATG AGGCAAGATC CTACGCGGAA GGGCTCAGTG 
    GTCAATGTCA ATCCCACTAA CACTCGGCCC CAGAGCGACA CGCCAGAGAT TCGCAAATAC 
    AAGAAGAGGT TTAACTCTGA GATCCTGTGC GCTGCCTTGT GGGGAGTGAA CTTGTTGGTG 
    GGTACTGAGA GTGGCCTGAT GCTGCTGGAC AGAAGTGGCC AAGGGAAGGT GTATCCTCTG 
    ATCAACAGAA GGCGATTTCA GCAGATGGAC GTCCTGGAAG GCTTGAACGT CTTGGTGACG 
    ATATCTGGGA AAAAGGATAA GTTACGTGTC TACTATTTGT CCTGGTTAAG AAATAAAATA 
    CTTCACAACG ACCCAGAGGT TGAGAAGAAG CAGGGCTGGA CGACAGTGGG GGACCTGGAA 
    GGCTGTGTGC ATTATAAAGT TGTAAAATAT GAAAGGATCA AATTTCTGGT CATTGCTTTG 
    AAGAACTCTG TGGAAGTCTA CGCGTGGGCA CCCAAGCCAT ATCACAAATT CATGGCCTTT 
    AAGTCGTTTG GCGAGCTGGT GCACAAGCCG TTGCTGGTGG ATCTCACTGT TGAGGAAGGC 
    CAGAGGCTGA AAGTGATCTA TGGATCCTGT GCTGGATTCC ATGCTGTTGA TGTGGACTCG 
    GGATCGGTCT ATGACATTTA CCTACCAACA CACATCCAAT GCAGCATCAA ACCCCACGCG 
    ATCATCATCC TCCCCAACAC AGACGGCATG GAGCTCCTGG TGTGCTATGA AGATGAAGGG 
    GTTTACGTAA ACACGTATGG GAGGATCACC AAGGATGTGG TTCTGCAGTG GGGAGAGATG 
    CCGACGTCTG TAGCATATAT TCGATCCAAT CAGACGATGG GCTGGGGAGA AAAGGCCATA 
    GAGATCCGAT CTGTGGAAAC TGGCCACTTG GATGGCGTGT TTATGCACAA AAGGGCTCAA 
    AGGCTGAAGT TCTTGTGTGA ACGCAATGAC AAGGTGTTCT TTGCCTCGGT CCGGTCTGGG 
    GGCAGCAGCC AGGTTTACTT CATGACTCTC GGCAGGACCT CTCTTCTGAG CTGGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    MAP4K4 ( XM_025260756.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025116541.1 Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.
    Price & Availability↑
    CloneID OBu45810
    Clone ID Related Accession (Same CDS sequence) XM_025260756.1
    Accession Version XM_025260756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Bubalus bubalis(water buffalo)
    Product mitogen-activated protein kinase kinase kinase kinase 4 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGCATCG AGCCAAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCGCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GACGTGCGGC CGCCGCACAA GGTGACGGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACG 
    ACGGACGAGG AGGACGACGA CGCGGAGCAG GAAGGGGCCG AGGAGCCCAC GTCTGGCCCG 
    GAGGACACCA GAGCCGCATC ATCTCTGAAC TTGAGCAACG GTGAAACGGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGGACA 
    ACAGTGACTT CTGTGGTGGG GTTTTCCTGT GATGGAATGA GACCCGAGGC CATGAGGCAA 
    GATCCTACGC GGAAGGGCTC AGTGGTCAAT GTCAATCCCA CTAACACTCG GCCCCAGAGC 
    GACACGCCAG AGATTCGCAA ATACAAGAAG AGGTTTAACT CTGAGATCCT GTGCGCTGCC 
    TTGTGGGGAG TGAACTTGTT GGTGGGTACT GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
    GGCCAAGGGA AGGTGTATCC TCTGATCAAC AGAAGGCGAT TTCAGCAGAT GGACGTCCTG 
    GAAGGCTTGA ACGTCTTGGT GACGATATCT GGGAAAAAGG ATAAGTTACG TGTCTACTAT 
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AACGACCCAG AGGTTGAGAA GAAGCAGGGC 
    TGGACGACAG TGGGGGACCT GGAAGGCTGT GTGCATTATA AAGTTGTAAA ATATGAAAGG 
    ATCAAATTTC TGGTCATTGC TTTGAAGAAC TCTGTGGAAG TCTACGCGTG GGCACCCAAG 
    CCATATCACA AATTCATGGC CTTTAAGTCG TTTGGCGAGC TGGTGCACAA GCCGTTGCTG 
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG CTGAAAGTGA TCTATGGATC CTGTGCTGGA 
    TTCCATGCTG TTGATGTGGA CTCGGGATCG GTCTATGACA TTTACCTACC AACACACGTA 
    AGAAAGAACC CACACTCTAT GATCCAATGC AGCATCAAAC CCCACGCGAT CATCATCCTC 
    CCCAACACAG ACGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTAAAC 
    ACGTATGGGA GGATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC GACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCACAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025116541.1
    CDS124..3588
    Translation

    Target ORF information:

    RefSeq Version XM_025260756.1
    Organism Bubalus bubalis(water buffalo)
    Definition Bubalus bubalis mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_025260756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCGAACG ACTCCCCTGC CAAAAGTCTG GTGGACATAG ACCTGTCCTC CCTGCGGGAT 
    CCGGCGGGGA TTTTCGAGCT GGTGGAAGTG GTGGGAAATG GCACCTACGG ACAAGTCTAT 
    AAGGGTCGAC ATGTTAAAAC AGGTCAGCTG GCAGCCATCA AAGTTATGGA TGTTACCGAG 
    GATGAAGAGG AGGAAATCAA ACTGGAGATA AATATGCTGA AGAAATATTC TCATCATAGA 
    AATATTGCAA CGTATTACGG TGCTTTCATT AAAAAGAGCC CCCCAGGACA CGATGACCAG 
    CTTTGGCTCG TTATGGAGTT CTGTGGGGCC GGATCCATCA CAGACCTGGT GAAGAACACC 
    AAGGGCAACA CGCTAAAGGA AGACTGGATA GCCTACATCT CCAGGGAGAT CCTGCGGGGC 
    CTGGCCCATC TCCACATCCA TCACGTGATC CACCGCGACA TCAAGGGCCA GAATGTGCTG 
    CTGACCGAGA ACGCAGAGGT GAAGCTGGTG GACTTCGGGG TGAGTGCCCA GCTGGACAGG 
    ACCGTGGGGA GGAGGAACAC GTTCATAGGC ACCCCATACT GGATGGCCCC TGAGGTCATT 
    GCCTGTGACG AGAACCCGGA CGCCACCTAC GATTACAGGA GTGACCTCTG GTCCTGTGGC 
    ATCACTGCCA TTGAGATGGC TGAAGGCGCT CCCCCTCTCT GCGACATGCA TCCCATGAGA 
    GCCCTGTTTC TCATCCCCCG GAATCCTCCT CCCCGGCTGA AGTCCAAAAA ATGGTCCAAG 
    AAGTTTTTTA GTTTTATAGA AGGATGCCTG GTGAAGAATT ACATGCAGCG GCCCTCCACC 
    GAGCAGCTTC TGAAGCATCC TTTCATTAGG GATCAGCCGA ACGAAAGGCA GGTTAGAATC 
    CAGCTTAAGG ACCATATAGA CCGGACCAGG AAGAAGAGAG GAGAGAAAGA TGAAACCGAG 
    TATGAGTACA GTGGGAGTGA GGAAGAGGAG GAGGAGGCGC CTGAGCAGGA GGGCGAGCCC 
    AGCTCCATCG TCAACGTGCC GGGGGAGTCG ACGCTGCGCC GGGACTTCCT GCGGCTCCAG 
    CAGGAGAACA AGGAGCGCTC CGAGGCCCTG AGGAGGCAGC AGCTCCTGCA GGAGCAGCAG 
    CTCCGCGAGC AGGAGGAGTA TAAACGGCAG CTGCTGGCGG AGAGGCAGAA GCGGATCGAG 
    CAGCAGAAGG AGCAGAGGCG GCGGCTGGAA GAGCAACAGA GGAGAGAGCG GGAAGCCAGA 
    AGGCAGCAGG AACGTGAGCA GCGGAGGAGG GAACAGGAGG AGAAGAGGCG GCTGGAGGAG 
    CTGGAGAGAC GACGGAAGGA GGAGGAGGAG CGGAGGCGGG CGGAGGAGGA GAAGAGGAGA 
    GTGGAGAGGG AGCAGGAATA CATCCGACGG CAGCTGGAAG AGGAACAGCG TCACCTGGAA 
    CTGCTTCAGC AGCAGCTGCT CCAGGAGCAG GCCATGCTAC TGCACGACCC CCGGAGGCCG 
    CCCCCGCCGC CAGACAGGAG CAAGGCTGGC CTGCACGAGC CCAAGGCCCC GCACGAGCCC 
    GCTGACCGGG CCCGCGAGGT CCCTGTGAGA ACAACGTCAC GGTCCCCCGT CCTGTCCCGC 
    CGGGATTCCC CACTGCAGGG CAGTGGGCAG CAGAGTAACC AAGCTGGTCA GAGAAACTCC 
    ACCAGCATCG AGCCAAGGCT GCTCTGGGAG AGAGTGGAGA AGCTGGCGCC CAGGCCAGGC 
    AGTGGCAGCT CCTCGGGGTC CAGCAACTCA GGGTCCCAGC CCGGCTCCCA CCCAGGGTCT 
    CAGAGTGGCT CCGGGGAGCG CTTCAGAGTG AGATCATCAT CCAAATCTGA AGGCTCGCCA 
    TCTCAGCGCC TTGAAAACGC AGCAAAAAAG CCTGAAGATA AAAAGGAAGT GTTTAGACCT 
    CTCAAGCCTG CTGGTGAAGT GGACCTGACG GCGCTGGCCA AAGAGCTGCG GGCCGTGGAG 
    GACGTGCGGC CGCCGCACAA GGTGACGGAC TACTCCTCCT CCAGCGAGGA GTCGGGGACG 
    ACGGACGAGG AGGACGACGA CGCGGAGCAG GAAGGGGCCG AGGAGCCCAC GTCTGGCCCG 
    GAGGACACCA GAGCCGCATC ATCTCTGAAC TTGAGCAACG GTGAAACGGA GTCCGTGAAA 
    ACCATGGTTG TCCATGACGA CGTGGAGAGT GAACCAGCCC TGACCCCGTC CAAAGAGGGC 
    ACGCTGATCG TCCGCCAGAC TCAGTCCGCT AGTAACACAC TCCAGAAACA CAAATCTTCC 
    TCCTCCTTTA CACCTTTTAT AGACCCCAGA TTACTACAGA TTTCTCCATC TAGCGGGACA 
    ACAGTGACTT CTGTGGTGGG GTTTTCCTGT GATGGAATGA GACCCGAGGC CATGAGGCAA 
    GATCCTACGC GGAAGGGCTC AGTGGTCAAT GTCAATCCCA CTAACACTCG GCCCCAGAGC 
    GACACGCCAG AGATTCGCAA ATACAAGAAG AGGTTTAACT CTGAGATCCT GTGCGCTGCC 
    TTGTGGGGAG TGAACTTGTT GGTGGGTACT GAGAGTGGCC TGATGCTGCT GGACAGAAGT 
    GGCCAAGGGA AGGTGTATCC TCTGATCAAC AGAAGGCGAT TTCAGCAGAT GGACGTCCTG 
    GAAGGCTTGA ACGTCTTGGT GACGATATCT GGGAAAAAGG ATAAGTTACG TGTCTACTAT 
    TTGTCCTGGT TAAGAAATAA AATACTTCAC AACGACCCAG AGGTTGAGAA GAAGCAGGGC 
    TGGACGACAG TGGGGGACCT GGAAGGCTGT GTGCATTATA AAGTTGTAAA ATATGAAAGG 
    ATCAAATTTC TGGTCATTGC TTTGAAGAAC TCTGTGGAAG TCTACGCGTG GGCACCCAAG 
    CCATATCACA AATTCATGGC CTTTAAGTCG TTTGGCGAGC TGGTGCACAA GCCGTTGCTG 
    GTGGATCTCA CTGTTGAGGA AGGCCAGAGG CTGAAAGTGA TCTATGGATC CTGTGCTGGA 
    TTCCATGCTG TTGATGTGGA CTCGGGATCG GTCTATGACA TTTACCTACC AACACACGTA 
    AGAAAGAACC CACACTCTAT GATCCAATGC AGCATCAAAC CCCACGCGAT CATCATCCTC 
    CCCAACACAG ACGGCATGGA GCTCCTGGTG TGCTATGAAG ATGAAGGGGT TTACGTAAAC 
    ACGTATGGGA GGATCACCAA GGATGTGGTT CTGCAGTGGG GAGAGATGCC GACGTCTGTA 
    GCATATATTC GATCCAATCA GACGATGGGC TGGGGAGAAA AGGCCATAGA GATCCGATCT 
    GTGGAAACTG GCCACTTGGA TGGCGTGTTT ATGCACAAAA GGGCTCAAAG GCTGAAGTTC 
    TTGTGTGAAC GCAATGACAA GGTGTTCTTT GCCTCGGTCC GGTCTGGGGG CAGCAGCCAG 
    GTTTACTTCA TGACTCTCGG CAGGACCTCT CTTCTGAGCT GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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