IL4 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL4
Entrez Gene ID	102397131
Full Name	interleukin 4
Synonyms	BSF-1,IL-4
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290959.1	NP_001277888.1	interleukin-4 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics.
Mingala CN, Odbileg R, Konnai S, Ohashi K, Onuma M
Veterinary immunology and immunopathology113(3-4)348-56(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu00150	NM_001290959.1 Latest version!	Bubalus bubalis interleukin 4 (IL4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL4 ( NM_001290959.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> NP_001277888.1 Bubalus bubalis interleukin 4 (IL4), mRNA.

Price & Availability↑

CloneID	OBu00150
Clone ID Related Accession (Same CDS sequence)	NM_001290959.1
Accession Version	NM_001290959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Bubalus bubalis(water buffalo)
Product	interleukin-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF407850.1. On Mar 20, 2014 this sequence version replaced XM_006046043.1. ##Evidence-Data-START## Transcript exon combination :: EF407850.1, AB246355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103886622, SAMEA103886803 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCTCA CCTCCCAGCT GATCCCAGTG CTGGTCTGCT TACTGGTATG TACCAGTCAC 
TTCGTCCATG GACACAAGTG CGATATTACC TTAGCAGAGA TCATCAAAAC GCTGAACATC 
CTCACAACGA GAAAGAATTC ATGCATGGAG CTGCCTGTAG CAGACGTCTT TGCTGCCCCA 
AAGAACACAA CTGAGAAGGA AACCTTCTGC AGGGTTGGAA TTGAGCTTAG GCGTATCTAC 
AGGAGCCACA CGTGCTTGAA CAAATTCCTG GGCGGACTCG ACAGGAATCT CAACAGCTTG 
GCAAGCAAGA CCTGTTCTGT GAATGAAGCC AAGACGAGCA CAAGTACGCT GAAAGACCTC 
TTGGAAAGGC TAAAGACGAT TATGAAGGAG AAATACTCCA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277888.1
CDS	59..466
Translation	MGLTSQLIPVLVCLLVCTSHFVHGHKCDITLAEIIKTLNILTTRKNSCMELPVADVFAAPKNTTEKETFCRVGIELRRIYRSHTCLNKFLGGLDRNLNSLASKTCSVNEAKTSTSTLKDLLERLKTIMKEKYSKC

Target ORF information:

RefSeq Version	NM_001290959.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis interleukin 4 (IL4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290959.1

ORF Insert Sequence:

ATGGGTCTCA CCTCCCAGCT GATCCCAGTG CTGGTCTGCT TACTGGTATG TACCAGTCAC 
TTCGTCCATG GACACAAGTG CGATATTACC TTAGCAGAGA TCATCAAAAC GCTGAACATC 
CTCACAACGA GAAAGAATTC ATGCATGGAG CTGCCTGTAG CAGACGTCTT TGCTGCCCCA 
AAGAACACAA CTGAGAAGGA AACCTTCTGC AGGGTTGGAA TTGAGCTTAG GCGTATCTAC 
AGGAGCCACA CGTGCTTGAA CAAATTCCTG GGCGGACTCG ACAGGAATCT CAACAGCTTG 
GCAAGCAAGA CCTGTTCTGT GAATGAAGCC AAGACGAGCA CAAGTACGCT GAAAGACCTC 
TTGGAAAGGC TAAAGACGAT TATGAAGGAG AAATACTCCA AGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics.
Veterinary immunology and immunopathology113(3-4)348-56(2006 Oct)
Mingala CN,Odbileg R,Konnai S,Ohashi K,Onuma M