WWC1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	WWC1
Entrez Gene ID	102392669
Full Name	WW and C2 domain containing 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025292279.1	XP_025148064.1	protein KIBRA
XM_006065122.1	XP_006065184.1	protein KIBRA

Pathways from BioSystems
Gene Ontology

The following WWC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WWC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu20711	XM_006065122.1 Latest version!	Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu71594	XM_025292279.1 Latest version!	Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WWC1 ( XM_006065122.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006065184.1 Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.

Price & Availability↑

CloneID	OBu20711
Clone ID Related Accession (Same CDS sequence)	XM_006065122.1
Accession Version	XM_006065122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	protein KIBRA
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785161.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTTGG TGGCCCTGGA CTCTGCAGGA GTCCCGGTGC TGGCTTGGTA CACCAAACCG 
CTCACCTTTG CTGACTGCAT TAGTGACGAG CTGCCGCTGG GATGGGAAGA GGCATACGAC 
CCACAGGTCG GAGATTACTT CATAGACCAC AACACCAAAA CCACTCAGAT CGAGGATCCC 
CGGATACAAT GGCGGCGGGA GCAGGAGCAC ATGCTGAAGG ATTACCTGGT GGTGGCCCAG 
GAGGCCCTGA GTGCACAAAA GGAAATCTAC CAGGTGAAGC AGCAGCGCTT AGAGCTTGCA 
CAGCAGGAGT ATCAGCAGCT GCATGCCGTC TGGGAGCACA AGCTTGGCTC CCAGGTCAGC 
TTGGTCTCTG GCTCGTCATC CAGCTCTAAG TATGACCCTG AGATTCTGAA AGCTGAAATT 
GCCACAGCAA AATCCCGGGT TAACAAGCTG AAGAGAGAGA TGGTTCACCT GCAACAGGAG 
CTACAGTTCA AAGAGCGAGG CTTTCAGACA CTGAAGAAAA TCGATAAGAA GATGTCAGAT 
GCTCAGGGCA GCTACAAACT GGATGAAGCT CAGGCTGTTC TGAGAGAAAC AAAAGCCATC 
AAAAAGGCCA TCACCTGTGG AGAAAAGGAA AAGCAAGATC TCATTAAGAG CCTCGCCATG 
CTGAAGGACG GCTTCCGCAC CAACAGAGGC TCCCACTCTG ACCTGTGGTC CAGCAGCAGC 
TCTCTGGAGA GTTCAGGTTT CCCACTGCCG AAACAGTACC TGGATGTGAG CTCCCAGACG 
GACATCTCAG GAAGTTTCGG TACCAGCAGC AACAATCAGT TGGCGGAGAA GGTCAGATTG 
CGCCTTCGAT ACGAAGAGGC CAAGAGAAGG ATTGCCAACC TGAAGATCCA GCTCGCCAAG 
CTCGACAGCG AGGCCTGGCC CGGAGTGCTC GACTCTGAGA GGGACCGGCT GATCCTCATC 
AATGAGAAGG AGGAGCTACT GAAGGAGATG CGCTTCATCA GCCCCCGGAA GTGGACCCAA 
GGGGAGGTGG AGCAGCTGGA GATGGCCCGC AAGCGGCTGG AGAAGGACCT GCAGGCGGCC 
CGGGACACCC AGAGCAAGGC GCTGACCGAG AGGTTGAAGT TAAACAGTAA AAGGAACCAG 
CTGGTGAGAG AGCTGGAGGA AGCCACCCGG CAGGTTGCAG CCCTGCACTC CCAGCTGAAG 
AGCCTCTCTG GCAGCATGCA GAGCCTGTCC TCCGGCAGCA GTCCGGGGTC CCTCACGTCC 
AGCCGGGGCT CTCTGGCCGC CTCCAGCCTG GACTCCTCTG CCTCAGCCAG TTTCACCGAC 
CTCTACTATG ACCCATTCGA GCAGCTGGAC TCGGAGCTGC AGAACAAGGT GGAATTCCTG 
CTCCTGGAGG GGGCCACGGG CTTCCGGCCC TCTGGCTGCA TCACCACCAT CCACGAGGAC 
GAAGTGGCCA AGACCCAGAA GGCGGAGGGG GGTGGTCGCC TGCAGGCCCT GCGCTCCCTA 
TCGGGCACCC CCAAGTCCAT GACCTCCCTG TCCCCCCGCT CCTCGCTCTC CTCCCCATCC 
CCGCCCTGCT CGCCTCTCAT CGCTGACTTG GCTGGGGAAG CCTTCCTGAG CAACCTGGAG 
TTTGAAGACC CCGAGCTGAG TGCCGCTCTC TGCGAGCTGA GCCTCGGCAG CAGCACGCGG 
GAGAGGTACC GGCTGGAGGA ACCCGGCCCG GAGGGCAAGC CGCTGGGCCA AGCTGTGAAT 
ATGGCTCAGG GGTGCGGCCT GAAAGTGGCC TGTGTCTCTG CTGCCGTGTC AGATGAGTCC 
GTGGCCGGAG ACAGTGGTGT GTATGAGGCT TCCGTGCAGA GGCTGGGTGT CTCGGAAGCC 
ACTGCTTTCG ACAGTGATGA ATCAGAAGCA GTGGGTGCCA CCCGGGTTCA GGTCGCCCTG 
AAGTATGATG AGAAGAATAA GCAGTTTGCA ATATTCATCA TCCAGCTGAG CAACCTTTCT 
GCTTTGTTGC TGCAGCAAGA CCAGAAAGTG AACATCCGAG CGGCTCTCCT TCCGTGCTCC 
GAGAGCACCA CCTGCCTCTT CCGGACCCGG CCCCTGGATG CTGCGGACAC CCTGGTGTTC 
AACGAGGTGT TCTGGGTGTC CATGTCCTAC CCAGCCCTCC ACCAGAAGAC CTTAAGAGTC 
GACGTCTGTA CCACCGACAG GTGCCATCTG GAGGAGTGCC TGGGAGGCGC CCAGATCAGC 
CTGGCTGAGG TCTGCCGGTC CGGTGAGAGG TCGACTCGGT GGTACAACCT CCTGAGCTAC 
AAATACTTGA AGAAACAGAG CCGGGAACCC AAACCAGTGG GAGCCATGGC CCCCATCCCA 
GGGCCTGAAA GCGCGGATGC TGTGTCTGCT CTGTTGAAAC AGACGGCCGT GGAGCTGGAG 
AAGAGGCAGG AGGAAAGGAG CAGCACACAG GCCCTGGAAG ACACCTGGAG GTATGAGGAG 
GGGACCAGCG AGAACGAGGC TGCAGCCGAG GAGGAGGAAG AGGAGGAAGA GGAGGAGGAA 
GGGGAAGAGG AAGCCTCCCC TGACATGGAC GGGTGCCTAG CGTTAAAGGT GGACAAGGAG 
ACCAACACAG AGACCCCAGC CCCATCTCCC ACGGTGGTGC GGCCCAAGGA CCGGCGGGTG 
GGCACGCCCT CCCCGGGGCC GTTTCTTCGA GGGAGCACCA TCATCCGTTC CAAGACCTTC 
TCTCCAGGCC CCCAGAGCCA GTACGTGTGC CGGCTGAACC GAAGCGATAG CGACAGCTCC 
ACCCTGTCCA AGAAGCCACC TTTTGTTCGA AATTCCTTGG AGCGGCGCAG CGTCCGGATG 
AAGCGGCCTT CATCTGTCAA ATCCCTGCGT ACTGAACGCC TGCCGCGCAC CTCGCTGGAC 
CTCGAGCTGG ACCTGCAGGC CACCAGGACC TGGCACAGCC AGCTGACTCA GGAGATCTCG 
GTGCTGAAGG AGCTCAAGGA GCAGCTGGAA CAAGCCAAGA GCCACGGGGA GAAGGAGCTG 
CCACAGTGGG TGCGGGAGGA CGAGCGCTTC CGCCTGCTGC TGAGGATGCT GGAGAAGCGG 
ATGGACCGAG CAGAGCACAA GGGAGAGCTT CAGACAGACA AGATGATGAG GTCAGCCGCC 
AAGGACGTGC ACAGGCTTCG AGGCCAGAGC TGTAAGGAGC CCCCGGAGGT GCAGTCTTTC 
AGGGAGAAGA TGGCATTTTT CACGCGGCCT CGGATGAATA TCCCGACCCT CTCTGCAGAT 
GACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006065184.1
CDS	1..3249
Translation	MCLVALDSAGVPVLAWYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRIQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQQELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFRTNRGSHSDLWSSSSSLESSGFPLPKQYLDVSSQTDISGSFGTSSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVAALHSQLKSLSGSMQSLSSGSSPGSLTSSRGSLAASSLDSSASASFTDLYYDPFEQLDSELQNKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLIADLAGEAFLSNLEFEDPELSAALCELSLGSSTRERYRLEEPGPEGKPLGQAVNMAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGVSEATAFDSDESEAVGATRVQVALKYDEKNKQFAIFIIQLSNLSALLLQQDQKVNIRAALLPCSESTTCLFRTRPLDAADTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRCHLEECLGGAQISLAEVCRSGERSTRWYNLLSYKYLKKQSREPKPVGAMAPIPGPESADAVSALLKQTAVELEKRQEERSSTQALEDTWRYEEGTSENEAAAEEEEEEEEEEEGEEEASPDMDGCLALKVDKETNTETPAPSPTVVRPKDRRVGTPSPGPFLRGSTIIRSKTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRTERLPRTSLDLELDLQATRTWHSQLTQEISVLKELKEQLEQAKSHGEKELPQWVREDERFRLLLRMLEKRMDRAEHKGELQTDKMMRSAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPTLSADDV

Target ORF information:

RefSeq Version	XM_006065122.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006065122.1

ORF Insert Sequence:

ATGTGCTTGG TGGCCCTGGA CTCTGCAGGA GTCCCGGTGC TGGCTTGGTA CACCAAACCG 
CTCACCTTTG CTGACTGCAT TAGTGACGAG CTGCCGCTGG GATGGGAAGA GGCATACGAC 
CCACAGGTCG GAGATTACTT CATAGACCAC AACACCAAAA CCACTCAGAT CGAGGATCCC 
CGGATACAAT GGCGGCGGGA GCAGGAGCAC ATGCTGAAGG ATTACCTGGT GGTGGCCCAG 
GAGGCCCTGA GTGCACAAAA GGAAATCTAC CAGGTGAAGC AGCAGCGCTT AGAGCTTGCA 
CAGCAGGAGT ATCAGCAGCT GCATGCCGTC TGGGAGCACA AGCTTGGCTC CCAGGTCAGC 
TTGGTCTCTG GCTCGTCATC CAGCTCTAAG TATGACCCTG AGATTCTGAA AGCTGAAATT 
GCCACAGCAA AATCCCGGGT TAACAAGCTG AAGAGAGAGA TGGTTCACCT GCAACAGGAG 
CTACAGTTCA AAGAGCGAGG CTTTCAGACA CTGAAGAAAA TCGATAAGAA GATGTCAGAT 
GCTCAGGGCA GCTACAAACT GGATGAAGCT CAGGCTGTTC TGAGAGAAAC AAAAGCCATC 
AAAAAGGCCA TCACCTGTGG AGAAAAGGAA AAGCAAGATC TCATTAAGAG CCTCGCCATG 
CTGAAGGACG GCTTCCGCAC CAACAGAGGC TCCCACTCTG ACCTGTGGTC CAGCAGCAGC 
TCTCTGGAGA GTTCAGGTTT CCCACTGCCG AAACAGTACC TGGATGTGAG CTCCCAGACG 
GACATCTCAG GAAGTTTCGG TACCAGCAGC AACAATCAGT TGGCGGAGAA GGTCAGATTG 
CGCCTTCGAT ACGAAGAGGC CAAGAGAAGG ATTGCCAACC TGAAGATCCA GCTCGCCAAG 
CTCGACAGCG AGGCCTGGCC CGGAGTGCTC GACTCTGAGA GGGACCGGCT GATCCTCATC 
AATGAGAAGG AGGAGCTACT GAAGGAGATG CGCTTCATCA GCCCCCGGAA GTGGACCCAA 
GGGGAGGTGG AGCAGCTGGA GATGGCCCGC AAGCGGCTGG AGAAGGACCT GCAGGCGGCC 
CGGGACACCC AGAGCAAGGC GCTGACCGAG AGGTTGAAGT TAAACAGTAA AAGGAACCAG 
CTGGTGAGAG AGCTGGAGGA AGCCACCCGG CAGGTTGCAG CCCTGCACTC CCAGCTGAAG 
AGCCTCTCTG GCAGCATGCA GAGCCTGTCC TCCGGCAGCA GTCCGGGGTC CCTCACGTCC 
AGCCGGGGCT CTCTGGCCGC CTCCAGCCTG GACTCCTCTG CCTCAGCCAG TTTCACCGAC 
CTCTACTATG ACCCATTCGA GCAGCTGGAC TCGGAGCTGC AGAACAAGGT GGAATTCCTG 
CTCCTGGAGG GGGCCACGGG CTTCCGGCCC TCTGGCTGCA TCACCACCAT CCACGAGGAC 
GAAGTGGCCA AGACCCAGAA GGCGGAGGGG GGTGGTCGCC TGCAGGCCCT GCGCTCCCTA 
TCGGGCACCC CCAAGTCCAT GACCTCCCTG TCCCCCCGCT CCTCGCTCTC CTCCCCATCC 
CCGCCCTGCT CGCCTCTCAT CGCTGACTTG GCTGGGGAAG CCTTCCTGAG CAACCTGGAG 
TTTGAAGACC CCGAGCTGAG TGCCGCTCTC TGCGAGCTGA GCCTCGGCAG CAGCACGCGG 
GAGAGGTACC GGCTGGAGGA ACCCGGCCCG GAGGGCAAGC CGCTGGGCCA AGCTGTGAAT 
ATGGCTCAGG GGTGCGGCCT GAAAGTGGCC TGTGTCTCTG CTGCCGTGTC AGATGAGTCC 
GTGGCCGGAG ACAGTGGTGT GTATGAGGCT TCCGTGCAGA GGCTGGGTGT CTCGGAAGCC 
ACTGCTTTCG ACAGTGATGA ATCAGAAGCA GTGGGTGCCA CCCGGGTTCA GGTCGCCCTG 
AAGTATGATG AGAAGAATAA GCAGTTTGCA ATATTCATCA TCCAGCTGAG CAACCTTTCT 
GCTTTGTTGC TGCAGCAAGA CCAGAAAGTG AACATCCGAG CGGCTCTCCT TCCGTGCTCC 
GAGAGCACCA CCTGCCTCTT CCGGACCCGG CCCCTGGATG CTGCGGACAC CCTGGTGTTC 
AACGAGGTGT TCTGGGTGTC CATGTCCTAC CCAGCCCTCC ACCAGAAGAC CTTAAGAGTC 
GACGTCTGTA CCACCGACAG GTGCCATCTG GAGGAGTGCC TGGGAGGCGC CCAGATCAGC 
CTGGCTGAGG TCTGCCGGTC CGGTGAGAGG TCGACTCGGT GGTACAACCT CCTGAGCTAC 
AAATACTTGA AGAAACAGAG CCGGGAACCC AAACCAGTGG GAGCCATGGC CCCCATCCCA 
GGGCCTGAAA GCGCGGATGC TGTGTCTGCT CTGTTGAAAC AGACGGCCGT GGAGCTGGAG 
AAGAGGCAGG AGGAAAGGAG CAGCACACAG GCCCTGGAAG ACACCTGGAG GTATGAGGAG 
GGGACCAGCG AGAACGAGGC TGCAGCCGAG GAGGAGGAAG AGGAGGAAGA GGAGGAGGAA 
GGGGAAGAGG AAGCCTCCCC TGACATGGAC GGGTGCCTAG CGTTAAAGGT GGACAAGGAG 
ACCAACACAG AGACCCCAGC CCCATCTCCC ACGGTGGTGC GGCCCAAGGA CCGGCGGGTG 
GGCACGCCCT CCCCGGGGCC GTTTCTTCGA GGGAGCACCA TCATCCGTTC CAAGACCTTC 
TCTCCAGGCC CCCAGAGCCA GTACGTGTGC CGGCTGAACC GAAGCGATAG CGACAGCTCC 
ACCCTGTCCA AGAAGCCACC TTTTGTTCGA AATTCCTTGG AGCGGCGCAG CGTCCGGATG 
AAGCGGCCTT CATCTGTCAA ATCCCTGCGT ACTGAACGCC TGCCGCGCAC CTCGCTGGAC 
CTCGAGCTGG ACCTGCAGGC CACCAGGACC TGGCACAGCC AGCTGACTCA GGAGATCTCG 
GTGCTGAAGG AGCTCAAGGA GCAGCTGGAA CAAGCCAAGA GCCACGGGGA GAAGGAGCTG 
CCACAGTGGG TGCGGGAGGA CGAGCGCTTC CGCCTGCTGC TGAGGATGCT GGAGAAGCGG 
ATGGACCGAG CAGAGCACAA GGGAGAGCTT CAGACAGACA AGATGATGAG GTCAGCCGCC 
AAGGACGTGC ACAGGCTTCG AGGCCAGAGC TGTAAGGAGC CCCCGGAGGT GCAGTCTTTC 
AGGGAGAAGA TGGCATTTTT CACGCGGCCT CGGATGAATA TCCCGACCCT CTCTGCAGAT 
GACGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WWC1 ( XM_025292279.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025148064.1 Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.

Price & Availability↑

CloneID	OBu71594
Clone ID Related Accession (Same CDS sequence)	XM_025292279.1
Accession Version	XM_025292279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	protein KIBRA
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCGGC CGGAGCTGCC CCTGCCGGAG GGCTGGGAGG AGGCGCGCGA CTTCGACGGC 
AAGGTCTACT ACATAGACCA CACGAGCCGC ACCACCAGCT GGATCGACCC GCGGGACAGG 
TACACCAAAC CGCTCACCTT TGCTGACTGC ATTAGTGACG AGCTGCCGCT GGGATGGGAA 
GAGGCATACG ACCCACAGGT CGGAGATTAC TTCATAGACC ACAACACCAA AACCACTCAG 
ATCGAGGATC CCCGGATACA ATGGCGGCGG GAGCAGGAGC ACATGCTGAA GGATTACCTG 
GTGGTGGCCC AGGAGGCCCT GAGTGCACAA AAGGAAATCT ACCAGGTGAA GCAGCAGCGC 
TTAGAGCTTG CACAGCAGGA GTATCAGCAG CTGCATGCCG TCTGGGAGCA CAAGCTTGGC 
TCCCAGGTCA GCTTGGTCTC TGGCTCGTCA TCCAGCTCTA AGTATGACCC TGAGATTCTG 
AAAGCTGAAA TTGCCACAGC AAAATCCCGG GTTAACAAGC TGAAGAGAGA GATGGTTCAC 
CTGCAACAGG AGCTACAGTT CAAAGAGCGA GGCTTTCAGA CACTGAAGAA AATCGATAAG 
AAGATGTCAG ATGCTCAGGG CAGCTACAAA CTGGATGAAG CTCAGGCTGT TCTGAGAGAA 
ACAAAAGCCA TCAAAAAGGC CATCACCTGT GGAGAAAAGG AAAAGCAAGA TCTCATTAAG 
AGCCTCGCCA TGCTGAAGGA CGGCTTCCAC ACCGACAGAG GCTCCCACTC TGACCTGTGG 
TCCAGCAGCA GCTCTCTGGA GAGTTCAGGT TTCCCACTGC CGAAACAGTA CCTGGATGTG 
AGCTCCCAGA CGGACATCTC AGGAAGTTTC GGTACCAGCA GCAACAATCA GTTGGCGGAG 
AAGGTCAGAT TGCGCCTTCG ATACGAAGAG GCCAAGAGAA GGATTGCCAA CCTGAAGATC 
CAGCTCGCCA AGCTCGACAG CGAGGCCTGG CCCGGAGTGC TCGACTCTGA GAGGGACCGG 
CTGATCCTCA TCAATGAGAA GGAGGAGCTA CTGAAGGAGA TGCGCTTCAT CAGCCCCCGG 
AAGTGGACCC AAGGGGAGGT GGAGCAGCTG GAGATGGCCC GCAAGCGGCT GGAGAAGGAC 
CTGCAGGCGG CCCGGGACAC CCAGAGCAAG GCGCTGACCG AGAGGTTGAA GTTAAACAGT 
AAAAGGAACC AGCTGGTGAG AGAGCTGGAG GAAGCCACCC GGCAGGTTGC AGCCCTGCAC 
TCCCAGCTGA AGAGCCTCTC TGGCAGCATG CAGAGCCTGT CCTCCGGCAG CAGTCCGGGG 
TCCCTCACGT CCAGCCGGGG CTCTCTGGCC GCCTCCAGCC TGGACTCCTC TGCCTCAGCC 
AGTTTCACCG ACCTCTACTA TGACCCATTC GAGCAGCTGG ACTCGGAGCT GCAGAACAAG 
GTGGAATTCC TGCTCCTGGA GGGGGCCACG GGCTTCCGGC CCTCTGGCTG CATCACCACC 
ATCCACGAGG ACGAAGTGGC CAAGACCCAG AAGGCGGAGG GGGGTGGTCG CCTGCAGGCC 
CTGCGCTCCC TATCGGGCAC CCCCAAGTCC ATGACCTCCC TGTCCCCCCG CTCCTCGCTC 
TCCTCCCCAT CCCCACCCTG CTCGCCTCTC ATCGCTGACT TGGCTGGGGA AGCCTTCCTG 
AGCAACCTGG AGTTTGAAGA CCCCGAGCTG AGTGCCGCTC TCTGCGAGCT GAGCCTCGGC 
AGCAGCACGC GGGAGAGGTA CCGGCTGGAG GAACCTGGCC CGGAGGGCAA GCCGCTGGGC 
CAAGCTGTGA ATATGGCTCA GGGGTGCGGC CTGAAAGTGG CCTGTGTCTC TGCTGCCGTG 
TCAGATGAGT CCGTGGCCGG AGACAGTGGT GTGTATGAGG CTTCCGTGCA GAGGCTGGGT 
GTCTCGGAAG CCACTGCTTT CGACAGTGAT GAATCAGAAG CAGTGGGTGC CACCCGGGTT 
CAGGTCGCCC TGAAGTATGA TGAGAAGAAT AAGCAGTTTG CAATATTCAT CATCCAGCTG 
AGCAACCTTT CTGCTTTGTT GCTGCAGCAA GACCAGAAAG TGAACATCCG AGCGGCTCTC 
CTTCCGTGCT CCGAGAGCAC CACCTGCCTC TTCCGGACCC GGCCCCTGGA TGCTGCGGAC 
ACCCTGGTGT TCAACGAGGT GTTCTGGGTG TCCATGTCCT ACCCAGCCCT CCACCAGAAG 
ACCTTAAGAG TCGACGTCTG TACCACCGAC AGGTGCCATC TGGAGGAGTG CCTGGGAGGC 
GCCCAGATCA GCCTGGCTGA GGTCTGCCGG TCCGGTGAGA GGTCGACTCG GTGGTACAAC 
CTCCTGAGCT ACAAATACTT GAAGAAACAG AGCCGGGAAC CCAAACCAGT GGGAGCCATG 
GCCCCCATCC CAGGGCCTGA AAGCGCGGAT GCTGTGTCTG CTCTGTTGGA ACAGACGGCC 
GTGGAGCTGG AGAAGAGGCA GGAGGAAAGG AGCAGCACAC AGGCCCTGGA AGACACCTGG 
AGGTATGAGG AGGGGACCAG CGAGAACGAG GCTGCAGCCG AGGAGGAGGA AGAGGAGGAA 
GAGGAGGAGG AAGGGGAAGA GGAAGCCTCC CCTGACATGG ACGGGTGCCT AGCGTTAAAG 
GTGGACAAGG AGACCAACAC AGAGACCCCA GCCCCATCTC CCACGGTGGT GCGGCCCAAG 
GACCGGCGGG TGGGCACGCC CTCCCCGGGG CCGTTTCTTC GAGGGAGCAC CATCATCCGT 
TCCAAGACCT TCTCTCCAGG CCCCCAGAGC CAGTACGTGT GCCGGCTGAA CCGAAGCGAT 
AGCGACAGCT CCACCCTGTC CAAGAAGCCA CCTTTTGTTC GAAATTCCTT GGAGCGGCGC 
AGCGTCCGGA TGAAGCGGCC TTCATCTGTC AAATCCCTGC GTACTGAACG CCTGCCGCGC 
ACCTCGCTGG ACCTCGAGCT GGACCTGCAG GCCACCAGGA CCTGGCACAG CCAGCTGACT 
CAGGAGATCT CGGTGCTGAA GGAGCTCAAG GAGCAGCTGG AACAAGCCAA GAGCCACGGG 
GAGAAGGAGC TGCCACAGTG GGTGCGGGAG GACGAGCGCT TCCGCCTGCT GCTGAGGATG 
CTGGAGAAGC GGATGGACCG AGCAGAGCAC AAGGGAGAGC TTCAGACAGA CAAGATGATG 
AGGTCAGCCG CCAAGGACGT GCACAGGCTT CGAGGCCAGA GCTGTAAGGA GCCCCCGGAG 
GTGCAGTCTT TCAGGGAGAA GATGGCATTT TTCACGCGGC CTCGGATGAA TATCCCGACC 
CTCTCTGCAG ATGACGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025148064.1
CDS	462..3782
Translation	MPRPELPLPEGWEEARDFDGKVYYIDHTSRTTSWIDPRDRYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRIQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQQELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFHTDRGSHSDLWSSSSSLESSGFPLPKQYLDVSSQTDISGSFGTSSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVAALHSQLKSLSGSMQSLSSGSSPGSLTSSRGSLAASSLDSSASASFTDLYYDPFEQLDSELQNKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLIADLAGEAFLSNLEFEDPELSAALCELSLGSSTRERYRLEEPGPEGKPLGQAVNMAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGVSEATAFDSDESEAVGATRVQVALKYDEKNKQFAIFIIQLSNLSALLLQQDQKVNIRAALLPCSESTTCLFRTRPLDAADTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRCHLEECLGGAQISLAEVCRSGERSTRWYNLLSYKYLKKQSREPKPVGAMAPIPGPESADAVSALLEQTAVELEKRQEERSSTQALEDTWRYEEGTSENEAAAEEEEEEEEEEEGEEEASPDMDGCLALKVDKETNTETPAPSPTVVRPKDRRVGTPSPGPFLRGSTIIRSKTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRTERLPRTSLDLELDLQATRTWHSQLTQEISVLKELKEQLEQAKSHGEKELPQWVREDERFRLLLRMLEKRMDRAEHKGELQTDKMMRSAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPTLSADDV

Target ORF information:

RefSeq Version	XM_025292279.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis WW and C2 domain containing 1 (WWC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025292279.1

ORF Insert Sequence:

ATGCCCCGGC CGGAGCTGCC CCTGCCGGAG GGCTGGGAGG AGGCGCGCGA CTTCGACGGC 
AAGGTCTACT ACATAGACCA CACGAGCCGC ACCACCAGCT GGATCGACCC GCGGGACAGG 
TACACCAAAC CGCTCACCTT TGCTGACTGC ATTAGTGACG AGCTGCCGCT GGGATGGGAA 
GAGGCATACG ACCCACAGGT CGGAGATTAC TTCATAGACC ACAACACCAA AACCACTCAG 
ATCGAGGATC CCCGGATACA ATGGCGGCGG GAGCAGGAGC ACATGCTGAA GGATTACCTG 
GTGGTGGCCC AGGAGGCCCT GAGTGCACAA AAGGAAATCT ACCAGGTGAA GCAGCAGCGC 
TTAGAGCTTG CACAGCAGGA GTATCAGCAG CTGCATGCCG TCTGGGAGCA CAAGCTTGGC 
TCCCAGGTCA GCTTGGTCTC TGGCTCGTCA TCCAGCTCTA AGTATGACCC TGAGATTCTG 
AAAGCTGAAA TTGCCACAGC AAAATCCCGG GTTAACAAGC TGAAGAGAGA GATGGTTCAC 
CTGCAACAGG AGCTACAGTT CAAAGAGCGA GGCTTTCAGA CACTGAAGAA AATCGATAAG 
AAGATGTCAG ATGCTCAGGG CAGCTACAAA CTGGATGAAG CTCAGGCTGT TCTGAGAGAA 
ACAAAAGCCA TCAAAAAGGC CATCACCTGT GGAGAAAAGG AAAAGCAAGA TCTCATTAAG 
AGCCTCGCCA TGCTGAAGGA CGGCTTCCAC ACCGACAGAG GCTCCCACTC TGACCTGTGG 
TCCAGCAGCA GCTCTCTGGA GAGTTCAGGT TTCCCACTGC CGAAACAGTA CCTGGATGTG 
AGCTCCCAGA CGGACATCTC AGGAAGTTTC GGTACCAGCA GCAACAATCA GTTGGCGGAG 
AAGGTCAGAT TGCGCCTTCG ATACGAAGAG GCCAAGAGAA GGATTGCCAA CCTGAAGATC 
CAGCTCGCCA AGCTCGACAG CGAGGCCTGG CCCGGAGTGC TCGACTCTGA GAGGGACCGG 
CTGATCCTCA TCAATGAGAA GGAGGAGCTA CTGAAGGAGA TGCGCTTCAT CAGCCCCCGG 
AAGTGGACCC AAGGGGAGGT GGAGCAGCTG GAGATGGCCC GCAAGCGGCT GGAGAAGGAC 
CTGCAGGCGG CCCGGGACAC CCAGAGCAAG GCGCTGACCG AGAGGTTGAA GTTAAACAGT 
AAAAGGAACC AGCTGGTGAG AGAGCTGGAG GAAGCCACCC GGCAGGTTGC AGCCCTGCAC 
TCCCAGCTGA AGAGCCTCTC TGGCAGCATG CAGAGCCTGT CCTCCGGCAG CAGTCCGGGG 
TCCCTCACGT CCAGCCGGGG CTCTCTGGCC GCCTCCAGCC TGGACTCCTC TGCCTCAGCC 
AGTTTCACCG ACCTCTACTA TGACCCATTC GAGCAGCTGG ACTCGGAGCT GCAGAACAAG 
GTGGAATTCC TGCTCCTGGA GGGGGCCACG GGCTTCCGGC CCTCTGGCTG CATCACCACC 
ATCCACGAGG ACGAAGTGGC CAAGACCCAG AAGGCGGAGG GGGGTGGTCG CCTGCAGGCC 
CTGCGCTCCC TATCGGGCAC CCCCAAGTCC ATGACCTCCC TGTCCCCCCG CTCCTCGCTC 
TCCTCCCCAT CCCCACCCTG CTCGCCTCTC ATCGCTGACT TGGCTGGGGA AGCCTTCCTG 
AGCAACCTGG AGTTTGAAGA CCCCGAGCTG AGTGCCGCTC TCTGCGAGCT GAGCCTCGGC 
AGCAGCACGC GGGAGAGGTA CCGGCTGGAG GAACCTGGCC CGGAGGGCAA GCCGCTGGGC 
CAAGCTGTGA ATATGGCTCA GGGGTGCGGC CTGAAAGTGG CCTGTGTCTC TGCTGCCGTG 
TCAGATGAGT CCGTGGCCGG AGACAGTGGT GTGTATGAGG CTTCCGTGCA GAGGCTGGGT 
GTCTCGGAAG CCACTGCTTT CGACAGTGAT GAATCAGAAG CAGTGGGTGC CACCCGGGTT 
CAGGTCGCCC TGAAGTATGA TGAGAAGAAT AAGCAGTTTG CAATATTCAT CATCCAGCTG 
AGCAACCTTT CTGCTTTGTT GCTGCAGCAA GACCAGAAAG TGAACATCCG AGCGGCTCTC 
CTTCCGTGCT CCGAGAGCAC CACCTGCCTC TTCCGGACCC GGCCCCTGGA TGCTGCGGAC 
ACCCTGGTGT TCAACGAGGT GTTCTGGGTG TCCATGTCCT ACCCAGCCCT CCACCAGAAG 
ACCTTAAGAG TCGACGTCTG TACCACCGAC AGGTGCCATC TGGAGGAGTG CCTGGGAGGC 
GCCCAGATCA GCCTGGCTGA GGTCTGCCGG TCCGGTGAGA GGTCGACTCG GTGGTACAAC 
CTCCTGAGCT ACAAATACTT GAAGAAACAG AGCCGGGAAC CCAAACCAGT GGGAGCCATG 
GCCCCCATCC CAGGGCCTGA AAGCGCGGAT GCTGTGTCTG CTCTGTTGGA ACAGACGGCC 
GTGGAGCTGG AGAAGAGGCA GGAGGAAAGG AGCAGCACAC AGGCCCTGGA AGACACCTGG 
AGGTATGAGG AGGGGACCAG CGAGAACGAG GCTGCAGCCG AGGAGGAGGA AGAGGAGGAA 
GAGGAGGAGG AAGGGGAAGA GGAAGCCTCC CCTGACATGG ACGGGTGCCT AGCGTTAAAG 
GTGGACAAGG AGACCAACAC AGAGACCCCA GCCCCATCTC CCACGGTGGT GCGGCCCAAG 
GACCGGCGGG TGGGCACGCC CTCCCCGGGG CCGTTTCTTC GAGGGAGCAC CATCATCCGT 
TCCAAGACCT TCTCTCCAGG CCCCCAGAGC CAGTACGTGT GCCGGCTGAA CCGAAGCGAT 
AGCGACAGCT CCACCCTGTC CAAGAAGCCA CCTTTTGTTC GAAATTCCTT GGAGCGGCGC 
AGCGTCCGGA TGAAGCGGCC TTCATCTGTC AAATCCCTGC GTACTGAACG CCTGCCGCGC 
ACCTCGCTGG ACCTCGAGCT GGACCTGCAG GCCACCAGGA CCTGGCACAG CCAGCTGACT 
CAGGAGATCT CGGTGCTGAA GGAGCTCAAG GAGCAGCTGG AACAAGCCAA GAGCCACGGG 
GAGAAGGAGC TGCCACAGTG GGTGCGGGAG GACGAGCGCT TCCGCCTGCT GCTGAGGATG 
CTGGAGAAGC GGATGGACCG AGCAGAGCAC AAGGGAGAGC TTCAGACAGA CAAGATGATG 
AGGTCAGCCG CCAAGGACGT GCACAGGCTT CGAGGCCAGA GCTGTAAGGA GCCCCCGGAG 
GTGCAGTCTT TCAGGGAGAA GATGGCATTT TTCACGCGGC CTCGGATGAA TATCCCGACC 
CTCTCTGCAG ATGACGTCTA A