LOC103852453 cDNA ORF clone, Brassica rapa(field mustard)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103852453
Entrez Gene ID	103852453
Full Name	biotin--protein ligase 2-like
Gene Type	protein-coding
Organism	Brassica rapa(field mustard)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009129359.2	XP_009127607.1	biotin--protein ligase 2-like

Pathways from BioSystems
Gene Ontology

The following LOC103852453 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103852453 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr134225	XM_009129359.2 Latest version!	Brassica rapa biotin--protein ligase 2-like (LOC103852453), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103852453 ( XM_009129359.2 ) cDNA ORF clone, Brassica rapa(field mustard) -> XP_009127607.1 Brassica rapa biotin--protein ligase 2-like (LOC103852453), mRNA.

Price & Availability↑

CloneID	OBr134225
Clone ID Related Accession (Same CDS sequence)	XM_009129359.2
Accession Version	XM_009129359.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-12
Organism	Brassica rapa(field mustard)
Product	biotin--protein ligase 2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024796.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 13, 2016 this sequence version replaced XM_009129359.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brassica rapa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGG CGAACTTGTG GCGTTTCGTT TCTTTAAGGC CTTCTTGGCG TCTATCTTTC 
TCCGTCGCTG CCACAGCAAT GGATGGTGAT CTAGATTCGT GTAGCTTGGT TTTGTGTGGG 
AAGTCCTGTG TTGAGAATGA TACAGCAAAG CGTTTGAAGA GCGAGAACGT GCTTAAGCTC 
CCAGATGATA CTACTAAAGT TTCACTCTTT CTAGATTCCG AGATTAATAA TTTGGTTAGA 
GATGGTGATT CATTCAATCC TTCACTCTTC ATGAACTCGC TTTCAACTGC TCGCTTTGGT 
CGGTTTCTCA TCTGGTCTCC CCGCTTGTCT TCCACTCACG ATCTTGTTTC TCATAACTTT 
TCTGAGCTTC CGGTTGGTTC AGTTTGTGTC TCAGATATTC AGTTCAAGGG TAGAGGCAGA 
ACAAAGAATG TGTGGGAATC TCCAAAGGGT TGTCTTATGT ATTCTTTTAC GGTAGAAATG 
GAAGATGGTC GAATCGTGCC TTTGATTCAA TACGTTGTAT CTCTTGCTGT AACCGAGGCA 
GTGAAAGATG TTTGTGACAA CAAGGGTTTG CCGTATATAG ACGTTAAAAT AAAGTGGCCC 
AATGATCTTT ACTTGAATGG CCTTAAAGTC GGAGGCATTT TGTGTACCTC TACCTACAGA 
TCTAGAATAT TTCACGTTAG TGTTGGTGTG GGATTGAATG TGGACAACAA TCAACCGACC 
ACATGCTTAA ATGCTGTACT AAAAGATATA TCTCCTGCAT CAGTTCTACT TAAAAGAGAA 
GAAATTATTG CTGCCTTCTT TCATAAATTC GAAAAATTCT TTGATCTATT TATTGACCAA 
GGTTTTAAAT CTCTCGAGGA GCTTTACTAC AGGACATGGC TTCACAGCGG CCAAAGAGTG 
ATCGTTGAAG ATAAAATCGA GGACCAAGTT GTTCAAAACG TTGTGACTAT CCAGGGTTTG 
ACATCTTCGG GATATCTGCT GGCTATTGGA GATGATTATC AAATGTATGA GCTTCACCCT 
GATGGCAATA GCTTTGACTT TTTCAAAGGT CTAGTCCGAA GAAAAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009127607.1
CDS	64..1134
Translation	MQVANLWRFVSLRPSWRLSFSVAATAMDGDLDSCSLVLCGKSCVENDTAKRLKSENVLKLPDDTTKVSLFLDSEINNLVRDGDSFNPSLFMNSLSTARFGRFLIWSPRLSSTHDLVSHNFSELPVGSVCVSDIQFKGRGRTKNVWESPKGCLMYSFTVEMEDGRIVPLIQYVVSLAVTEAVKDVCDNKGLPYIDVKIKWPNDLYLNGLKVGGILCTSTYRSRIFHVSVGVGLNVDNNQPTTCLNAVLKDISPASVLLKREEIIAAFFHKFEKFFDLFIDQGFKSLEELYYRTWLHSGQRVIVEDKIEDQVVQNVVTIQGLTSSGYLLAIGDDYQMYELHPDGNSFDFFKGLVRRKI

Target ORF information:

RefSeq Version	XM_009129359.2
Organism	Brassica rapa(field mustard)
Definition	Brassica rapa biotin--protein ligase 2-like (LOC103852453), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009129359.2

ORF Insert Sequence:

ATGCAAGTGG CGAACTTGTG GCGTTTCGTT TCTTTAAGGC CTTCTTGGCG TCTATCTTTC 
TCCGTCGCTG CCACAGCAAT GGATGGTGAT CTAGATTCGT GTAGCTTGGT TTTGTGTGGG 
AAGTCCTGTG TTGAGAATGA TACAGCAAAG CGTTTGAAGA GCGAGAACGT GCTTAAGCTC 
CCAGATGATA CTACTAAAGT TTCACTCTTT CTAGATTCCG AGATTAATAA TTTGGTTAGA 
GATGGTGATT CATTCAATCC TTCACTCTTC ATGAACTCGC TTTCAACTGC TCGCTTTGGT 
CGGTTTCTCA TCTGGTCTCC CCGCTTGTCT TCCACTCACG ATCTTGTTTC TCATAACTTT 
TCTGAGCTTC CGGTTGGTTC AGTTTGTGTC TCAGATATTC AGTTCAAGGG TAGAGGCAGA 
ACAAAGAATG TGTGGGAATC TCCAAAGGGT TGTCTTATGT ATTCTTTTAC GGTAGAAATG 
GAAGATGGTC GAATCGTGCC TTTGATTCAA TACGTTGTAT CTCTTGCTGT AACCGAGGCA 
GTGAAAGATG TTTGTGACAA CAAGGGTTTG CCGTATATAG ACGTTAAAAT AAAGTGGCCC 
AATGATCTTT ACTTGAATGG CCTTAAAGTC GGAGGCATTT TGTGTACCTC TACCTACAGA 
TCTAGAATAT TTCACGTTAG TGTTGGTGTG GGATTGAATG TGGACAACAA TCAACCGACC 
ACATGCTTAA ATGCTGTACT AAAAGATATA TCTCCTGCAT CAGTTCTACT TAAAAGAGAA 
GAAATTATTG CTGCCTTCTT TCATAAATTC GAAAAATTCT TTGATCTATT TATTGACCAA 
GGTTTTAAAT CTCTCGAGGA GCTTTACTAC AGGACATGGC TTCACAGCGG CCAAAGAGTG 
ATCGTTGAAG ATAAAATCGA GGACCAAGTT GTTCAAAACG TTGTGACTAT CCAGGGTTTG 
ACATCTTCGG GATATCTGCT GGCTATTGGA GATGATTATC AAATGTATGA GCTTCACCCT 
GATGGCAATA GCTTTGACTT TTTCAAAGGT CTAGTCCGAA GAAAAATATG A