LOC109472721 cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109472721
Entrez Gene ID	109472721
Full Name	endothelin-converting enzyme-like 1
Gene Type	protein-coding
Organism	Branchiostoma belcheri(Belcher's lancelet)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019772573.1	XP_019628132.1	endothelin-converting enzyme-like 1

Pathways from BioSystems
Gene Ontology

The following LOC109472721 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109472721 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr182314	XM_019772573.1 Latest version!	Branchiostoma belcheri endothelin-converting enzyme-like 1 (LOC109472721), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109472721 ( XM_019772573.1 ) cDNA ORF clone, Branchiostoma belcheri(Belcher's lancelet) -> XP_019628132.1 Branchiostoma belcheri endothelin-converting enzyme-like 1 (LOC109472721), mRNA.

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CloneID	OBr182314
Clone ID Related Accession (Same CDS sequence)	XM_019772573.1
Accession Version	XM_019772573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-12-27
Organism	Branchiostoma belcheri(Belcher's lancelet)
Product	endothelin-converting enzyme-like 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017803887.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Branchiostoma belcheri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGT CTGTGAACAA CGTGAAGCAC GGGGACCCGC ACGCCGAGGG CTCGCGGCAA 
GCGAAGGAAG CGGAGCTAAA TCACCCCGAA CCCGTCCGGT GCGTGATGCC GCGCGCGCCC 
AGGCCCAGGG AAGCAGCCGT GATGGTGTGT TTGTTCTTGT CTCTGACGGT TTGTGTGATT 
TTCGGCGTGA TGTTGGCGAC TCGAGGCGGG GTTCAGGAGG TAGGAGGCGT CCCTGACGGG 
CACGTCTGTC TCACGGCAGA CTGCATCACC TCAGCAGCCC TTCTGCTATC CAACATGGAC 
GCCACGGTCG ATCCCTGCCA GGACTTCTTC CAGTTTTCGT GCGGAGGTTG GCTCCAAGAG 
AACCCCATCC CTCCCGAGCA GAAGCAGTGG GGGGTCGACT CCAAGATGTG GGAAACTAAC 
GAGAGGATCT TGAGGAGGCT CCTCGCGACG CCGACTTTGA GAAACTCGAC GGAATCGTAC 
GAGCGGAAGG CGAAGGACTT CTTCCAGTCG TGCATGCAGA AGGAGGCCAG AGAACAGGCT 
TCGGGACGGC CTCTTCTGGA GCTGATGGAC CAGCTAGGAG GCTGGGCAGC CTTAGGGAAC 
TGGAGCGAGG CTACTTGGAA CTTTAACGAC ACTTTCACGA GGTTGCACCT GGATCTCAAG 
GTGGACGCGC TCTTCTTTTT ATGGGTGAAC TTCGACGATA AGGATTCGTC CAAGTACGTC 
TTCAGGATTG ACCAGGGAGG GCTGGGGCTT CCTGACAGGA GCTACTACAT CAGCCAGGAG 
TCACTGAAGG TAGTTGATGC GTACAGAAAG CTGATAGCCC GGGTTGTGGA ACTGCTGGGG 
GCAGACCCGG CATCGGCACA GGACTCTGCT CAAGATGTGG TCAACTTCGA GACCAAACTG 
GCAGAGCTGA CAGTTCCAGA GTCTGAACGA ACCAACGTCA ACGCGCTGTA CAACAACGTC 
CGGATTCACG AGCTGAAAAC TATCGCACCC ATGATCGACT GGGGTTCGTT TTTCCACGCC 
ATGGTTCCTG ACCTCACGCC GTCCGCGAGG GTGGTGCTGT ACGCTACCGA CTACCTGGGG 
CAGATCTCAC AACTCATCGC AGACACGCCG GCAAGGACGC TGAATAGCTA CCTCCTGTGG 
CGGGTTGCCG CCAGTTTCGT GACGGACCTC AGTCAGCCCT TCAGGAAGGC GCTGGAGAAC 
TTCAACAAGG TTGCTGAGGG GACGGCCGGC GTGACTGAGG AATGGCGGCT GTGTTTACAG 
GCGGTGGACG AACACATGGG CATGGCGCTG GGGGCCATGT TTGTCAAGGA CTCCTTCTCT 
AAGGAGAGCA AGGAAAAGGT GAGGGAGATT GCTGTTGACA TCAGACGAGC TTTCGTCTTC 
AACCTGGCTA AGATAGACTG GATGGATGAC AAGACCAAGG CGTCTGCCGT CGAGAAGTCG 
AACCACATGG TGGAGAAGAT TGGATATCCA GACTACATCC TGAATGACAC CCTGCTGGAT 
GAAAAGTTTA CAGAGCTGCG TGTAGGCAGA GAAAGTTTCT TCCAGAACCG GGTGAGCGGG 
ATGCGACATC AGGTGCGGCA GATGCTGGAC AAGCTAGAGA GACCGGTGGA CAGGACGCTT 
TGGACGATGC CACCCCATCT TGTGAATGCA TACTACTGGC CAAACACCAA CGAGATGGTT 
TTCCCTGCCG GTATACTACA GAGATCATTC TACGATTCAA AGTTCCCCAT GTCGATGAAC 
TTTGGGGGAA TCGGCGGAGT GATCGCTCAC GAACTGACTC ATGGGTACGA CGACTGGGGA 
GGAAACTATG ACAAGGAGGG GAACCTGAAG TCCTGGTGGT CCAACAGTTC CTGGACTAAC 
TTCACGCGTG CGTCACAGTG CGTCATCGAT CTGTACAACA GCTACGTCAT CGAAGGCAAA 
CAGATAGACG GCAGGCTTAC CCTGGGGGAA AATATAGCCG ACATCGGAGG CCTAAAGCAA 
GCATATTATG GATATAAGTT ATGGAGAAAA GTTCACCAGG ACGAGCCTCT ACTGCCCGGA 
CTGAACCGGT CACACGAGGA GGTGTTCTTC ATGTCGTTTG CACAGAGCTG GTGCATCACA 
CAGCGTCCTG AGGCGTTAGA CGTCATGTTA CTCGTGGATC CTCACGCACC ACCAAAGTAC 
AGGGTAATCG GCAGCGTGTC TCAGTTGGCA GAGTTCTCCG AGGCGTTCCA CTGTCCGGTG 
GGCAGCCCCA TGAACCCTGT ACACAAGTGT TCGGTTTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019628132.1
CDS	208..2469
Translation	MASSVNNVKHGDPHAEGSRQAKEAELNHPEPVRCVMPRAPRPREAAVMVCLFLSLTVCVIFGVMLATRGGVQEVGGVPDGHVCLTADCITSAALLLSNMDATVDPCQDFFQFSCGGWLQENPIPPEQKQWGVDSKMWETNERILRRLLATPTLRNSTESYERKAKDFFQSCMQKEAREQASGRPLLELMDQLGGWAALGNWSEATWNFNDTFTRLHLDLKVDALFFLWVNFDDKDSSKYVFRIDQGGLGLPDRSYYISQESLKVVDAYRKLIARVVELLGADPASAQDSAQDVVNFETKLAELTVPESERTNVNALYNNVRIHELKTIAPMIDWGSFFHAMVPDLTPSARVVLYATDYLGQISQLIADTPARTLNSYLLWRVAASFVTDLSQPFRKALENFNKVAEGTAGVTEEWRLCLQAVDEHMGMALGAMFVKDSFSKESKEKVREIAVDIRRAFVFNLAKIDWMDDKTKASAVEKSNHMVEKIGYPDYILNDTLLDEKFTELRVGRESFFQNRVSGMRHQVRQMLDKLERPVDRTLWTMPPHLVNAYYWPNTNEMVFPAGILQRSFYDSKFPMSMNFGGIGGVIAHELTHGYDDWGGNYDKEGNLKSWWSNSSWTNFTRASQCVIDLYNSYVIEGKQIDGRLTLGENIADIGGLKQAYYGYKLWRKVHQDEPLLPGLNRSHEEVFFMSFAQSWCITQRPEALDVMLLVDPHAPPKYRVIGSVSQLAEFSEAFHCPVGSPMNPVHKCSVW

Target ORF information:

RefSeq Version	XM_019772573.1
Organism	Branchiostoma belcheri(Belcher's lancelet)
Definition	Branchiostoma belcheri endothelin-converting enzyme-like 1 (LOC109472721), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019772573.1

ORF Insert Sequence:

ATGGCTTCGT CTGTGAACAA CGTGAAGCAC GGGGACCCGC ACGCCGAGGG CTCGCGGCAA 
GCGAAGGAAG CGGAGCTAAA TCACCCCGAA CCCGTCCGGT GCGTGATGCC GCGCGCGCCC 
AGGCCCAGGG AAGCAGCCGT GATGGTGTGT TTGTTCTTGT CTCTGACGGT TTGTGTGATT 
TTCGGCGTGA TGTTGGCGAC TCGAGGCGGG GTTCAGGAGG TAGGAGGCGT CCCTGACGGG 
CACGTCTGTC TCACGGCAGA CTGCATCACC TCAGCAGCCC TTCTGCTATC CAACATGGAC 
GCCACGGTCG ATCCCTGCCA GGACTTCTTC CAGTTTTCGT GCGGAGGTTG GCTCCAAGAG 
AACCCCATCC CTCCCGAGCA GAAGCAGTGG GGGGTCGACT CCAAGATGTG GGAAACTAAC 
GAGAGGATCT TGAGGAGGCT CCTCGCGACG CCGACTTTGA GAAACTCGAC GGAATCGTAC 
GAGCGGAAGG CGAAGGACTT CTTCCAGTCG TGCATGCAGA AGGAGGCCAG AGAACAGGCT 
TCGGGACGGC CTCTTCTGGA GCTGATGGAC CAGCTAGGAG GCTGGGCAGC CTTAGGGAAC 
TGGAGCGAGG CTACTTGGAA CTTTAACGAC ACTTTCACGA GGTTGCACCT GGATCTCAAG 
GTGGACGCGC TCTTCTTTTT ATGGGTGAAC TTCGACGATA AGGATTCGTC CAAGTACGTC 
TTCAGGATTG ACCAGGGAGG GCTGGGGCTT CCTGACAGGA GCTACTACAT CAGCCAGGAG 
TCACTGAAGG TAGTTGATGC GTACAGAAAG CTGATAGCCC GGGTTGTGGA ACTGCTGGGG 
GCAGACCCGG CATCGGCACA GGACTCTGCT CAAGATGTGG TCAACTTCGA GACCAAACTG 
GCAGAGCTGA CAGTTCCAGA GTCTGAACGA ACCAACGTCA ACGCGCTGTA CAACAACGTC 
CGGATTCACG AGCTGAAAAC TATCGCACCC ATGATCGACT GGGGTTCGTT TTTCCACGCC 
ATGGTTCCTG ACCTCACGCC GTCCGCGAGG GTGGTGCTGT ACGCTACCGA CTACCTGGGG 
CAGATCTCAC AACTCATCGC AGACACGCCG GCAAGGACGC TGAATAGCTA CCTCCTGTGG 
CGGGTTGCCG CCAGTTTCGT GACGGACCTC AGTCAGCCCT TCAGGAAGGC GCTGGAGAAC 
TTCAACAAGG TTGCTGAGGG GACGGCCGGC GTGACTGAGG AATGGCGGCT GTGTTTACAG 
GCGGTGGACG AACACATGGG CATGGCGCTG GGGGCCATGT TTGTCAAGGA CTCCTTCTCT 
AAGGAGAGCA AGGAAAAGGT GAGGGAGATT GCTGTTGACA TCAGACGAGC TTTCGTCTTC 
AACCTGGCTA AGATAGACTG GATGGATGAC AAGACCAAGG CGTCTGCCGT CGAGAAGTCG 
AACCACATGG TGGAGAAGAT TGGATATCCA GACTACATCC TGAATGACAC CCTGCTGGAT 
GAAAAGTTTA CAGAGCTGCG TGTAGGCAGA GAAAGTTTCT TCCAGAACCG GGTGAGCGGG 
ATGCGACATC AGGTGCGGCA GATGCTGGAC AAGCTAGAGA GACCGGTGGA CAGGACGCTT 
TGGACGATGC CACCCCATCT TGTGAATGCA TACTACTGGC CAAACACCAA CGAGATGGTT 
TTCCCTGCCG GTATACTACA GAGATCATTC TACGATTCAA AGTTCCCCAT GTCGATGAAC 
TTTGGGGGAA TCGGCGGAGT GATCGCTCAC GAACTGACTC ATGGGTACGA CGACTGGGGA 
GGAAACTATG ACAAGGAGGG GAACCTGAAG TCCTGGTGGT CCAACAGTTC CTGGACTAAC 
TTCACGCGTG CGTCACAGTG CGTCATCGAT CTGTACAACA GCTACGTCAT CGAAGGCAAA 
CAGATAGACG GCAGGCTTAC CCTGGGGGAA AATATAGCCG ACATCGGAGG CCTAAAGCAA 
GCATATTATG GATATAAGTT ATGGAGAAAA GTTCACCAGG ACGAGCCTCT ACTGCCCGGA 
CTGAACCGGT CACACGAGGA GGTGTTCTTC ATGTCGTTTG CACAGAGCTG GTGCATCACA 
CAGCGTCCTG AGGCGTTAGA CGTCATGTTA CTCGTGGATC CTCACGCACC ACCAAAGTAC 
AGGGTAATCG GCAGCGTGTC TCAGTTGGCA GAGTTCTCCG AGGCGTTCCA CTGTCCGGTG 
GGCAGCCCCA TGAACCCTGT ACACAAGTGT TCGGTTTGGT GA