LOC100826533 cDNA ORF clone, Brachypodium distachyon(stiff brome)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100826533
Entrez Gene ID	100826533
Full Name	villin-1-like
Gene Type	protein-coding
Organism	Brachypodium distachyon(stiff brome)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014898037.1	XP_014753523.1	villin-1-like isoform X3
XM_010233388.3	XP_010231690.1	villin-1 isoform X1
XM_014898036.1	XP_014753522.1	villin-1-like isoform X2
XM_014898037.2	XP_014753523.1	villin-1 isoform X3
XM_014898038.2	XP_014753524.1	villin-1 isoform X4
XM_010233388.2	XP_010231690.1	villin-1-like isoform X1
XM_014898038.1	XP_014753524.1	villin-1-like isoform X4
XM_014898036.2	XP_014753522.1	villin-1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC100826533 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100826533 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBr104084	XM_010233388.3 Latest version!	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBr104085	XM_014898036.2 Latest version!	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBr104086	XM_014898037.2 Latest version!	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBr104087	XM_014898038.2 Latest version!	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBr104086	XM_014898037.1	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBr104084	XM_010233388.2	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBr104087	XM_014898038.1	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBr104085	XM_014898036.1	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100826533 ( XM_010233388.3 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_010231690.1 Brachypodium distachyon villin-1 (LOC100826533), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBr104084
Clone ID Related Accession (Same CDS sequence)	XM_010233388.3 , XM_010233388.2
Accession Version	XM_010233388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_010233388.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG TGATCATGCT CTACTTGATA GGATGATGAA TAAGTTGTGG 
CCACTAAAGC AATCATTGTT AGTAAGAGAA GGTTCTGAGC CTGACCACTT CTGGAAAACA 
TTAGGAGGAA GATCAGAATA CTCTAAAGAA AAGCTTGTCA AGGGTTGGCC TGCAGATCCA 
CATATGTATA CTTGTACTTT CGAACAATGT CTGTTCAAGG CAAAGGAGGT GTTCAGCTTC 
ACGCAGGATG ATCTTGCAAC TGAGGAGACT TTGATTGTGG ACTGCAGCGA AGAAATCTAC 
GTCTGGGTCG GACTTCACTC CGGTGTCACG TCCAAGGAGC ATGCTCTTGA CATCGGCAAG 
ATGTTCCTTC AAGCAGGCAT TGGTCAGGAT GGACGGCGTT CAATCGACAC AACTGTATAT 
GCAGTTGCAG AAGGGGAGGA ACCTGCATTT TTCACCACCT TTTTCGACTG GGATAGTTCA 
AAGCAAGCAG AAGCTACGTT GGGGAACTCA TTTGAGAGGA AGCTCGCGAT GCTGAAAGGT 
CTTTCACCAA AATTGGAAGA GTCGTCGCCG GACACCAGGA TCAGCCTGCG CAAGTCACCG 
TCGAGAAGAC CTGCAGCTGC AGGGAGACCA TCGGAGCCAG CAGCCACGCC GGAGCAGCAA 
CCGTCAGCAA GGAAGATTGC GTTCGGCTCT GCCGGGAGGT TGGCTAGAGA GAGATCCCCA 
TCAGCGTCCG GACTACTGTC CCCATCGTCA GCATCATCGT CTCCTAAGAG CCGCGTGAAC 
TCCTCGCCGT CGACACCGGC GGTGGCGCGG CGGCTCTTCA GCAGCTCCTC AATGCACGCG 
GCGGCCGAGG CAGCAGCAGC TGCCTCGCAT GTCGCCTCCC CTACGGCCAA AGGATCACGA 
CGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010231690.1
CDS	162..2750
Translation	MATLKGVDDAFLGVGDKPGLDIWCVMGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_010233388.3
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010233388.3

ORF Insert Sequence:

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG TGATCATGCT CTACTTGATA GGATGATGAA TAAGTTGTGG 
CCACTAAAGC AATCATTGTT AGTAAGAGAA GGTTCTGAGC CTGACCACTT CTGGAAAACA 
TTAGGAGGAA GATCAGAATA CTCTAAAGAA AAGCTTGTCA AGGGTTGGCC TGCAGATCCA 
CATATGTATA CTTGTACTTT CGAACAATGT CTGTTCAAGG CAAAGGAGGT GTTCAGCTTC 
ACGCAGGATG ATCTTGCAAC TGAGGAGACT TTGATTGTGG ACTGCAGCGA AGAAATCTAC 
GTCTGGGTCG GACTTCACTC CGGTGTCACG TCCAAGGAGC ATGCTCTTGA CATCGGCAAG 
ATGTTCCTTC AAGCAGGCAT TGGTCAGGAT GGACGGCGTT CAATCGACAC AACTGTATAT 
GCAGTTGCAG AAGGGGAGGA ACCTGCATTT TTCACCACCT TTTTCGACTG GGATAGTTCA 
AAGCAAGCAG AAGCTACGTT GGGGAACTCA TTTGAGAGGA AGCTCGCGAT GCTGAAAGGT 
CTTTCACCAA AATTGGAAGA GTCGTCGCCG GACACCAGGA TCAGCCTGCG CAAGTCACCG 
TCGAGAAGAC CTGCAGCTGC AGGGAGACCA TCGGAGCCAG CAGCCACGCC GGAGCAGCAA 
CCGTCAGCAA GGAAGATTGC GTTCGGCTCT GCCGGGAGGT TGGCTAGAGA GAGATCCCCA 
TCAGCGTCCG GACTACTGTC CCCATCGTCA GCATCATCGT CTCCTAAGAG CCGCGTGAAC 
TCCTCGCCGT CGACACCGGC GGTGGCGCGG CGGCTCTTCA GCAGCTCCTC AATGCACGCG 
GCGGCCGAGG CAGCAGCAGC TGCCTCGCAT GTCGCCTCCC CTACGGCCAA AGGATCACGA 
CGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_010233388.2 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_010231690.1 Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBr104084
Clone ID Related Accession (Same CDS sequence)	XM_010233388.3 , XM_010233388.2
Accession Version	XM_010233388.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 8, 2015 this sequence version replaced XM_010233388.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG TGATCATGCT CTACTTGATA GGATGATGAA TAAGTTGTGG 
CCACTAAAGC AATCATTGTT AGTAAGAGAA GGTTCTGAGC CTGACCACTT CTGGAAAACA 
TTAGGAGGAA GATCAGAATA CTCTAAAGAA AAGCTTGTCA AGGGTTGGCC TGCAGATCCA 
CATATGTATA CTTGTACTTT CGAACAATGT CTGTTCAAGG CAAAGGAGGT GTTCAGCTTC 
ACGCAGGATG ATCTTGCAAC TGAGGAGACT TTGATTGTGG ACTGCAGCGA AGAAATCTAC 
GTCTGGGTCG GACTTCACTC CGGTGTCACG TCCAAGGAGC ATGCTCTTGA CATCGGCAAG 
ATGTTCCTTC AAGCAGGCAT TGGTCAGGAT GGACGGCGTT CAATCGACAC AACTGTATAT 
GCAGTTGCAG AAGGGGAGGA ACCTGCATTT TTCACCACCT TTTTCGACTG GGATAGTTCA 
AAGCAAGCAG AAGCTACGTT GGGGAACTCA TTTGAGAGGA AGCTCGCGAT GCTGAAAGGT 
CTTTCACCAA AATTGGAAGA GTCGTCGCCG GACACCAGGA TCAGCCTGCG CAAGTCACCG 
TCGAGAAGAC CTGCAGCTGC AGGGAGACCA TCGGAGCCAG CAGCCACGCC GGAGCAGCAA 
CCGTCAGCAA GGAAGATTGC GTTCGGCTCT GCCGGGAGGT TGGCTAGAGA GAGATCCCCA 
TCAGCGTCCG GACTACTGTC CCCATCGTCA GCATCATCGT CTCCTAAGAG CCGCGTGAAC 
TCCTCGCCGT CGACACCGGC GGTGGCGCGG CGGCTCTTCA GCAGCTCCTC AATGCACGCG 
GCGGCCGAGG CAGCAGCAGC TGCCTCGCAT GTCGCCTCCC CTACGGCCAA AGGATCACGA 
CGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010231690.1
CDS	107..2695
Translation	MATLKGVDDAFLGVGDKPGLDIWCVMGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_010233388.2
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010233388.2

ORF Insert Sequence:

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG TGATCATGCT CTACTTGATA GGATGATGAA TAAGTTGTGG 
CCACTAAAGC AATCATTGTT AGTAAGAGAA GGTTCTGAGC CTGACCACTT CTGGAAAACA 
TTAGGAGGAA GATCAGAATA CTCTAAAGAA AAGCTTGTCA AGGGTTGGCC TGCAGATCCA 
CATATGTATA CTTGTACTTT CGAACAATGT CTGTTCAAGG CAAAGGAGGT GTTCAGCTTC 
ACGCAGGATG ATCTTGCAAC TGAGGAGACT TTGATTGTGG ACTGCAGCGA AGAAATCTAC 
GTCTGGGTCG GACTTCACTC CGGTGTCACG TCCAAGGAGC ATGCTCTTGA CATCGGCAAG 
ATGTTCCTTC AAGCAGGCAT TGGTCAGGAT GGACGGCGTT CAATCGACAC AACTGTATAT 
GCAGTTGCAG AAGGGGAGGA ACCTGCATTT TTCACCACCT TTTTCGACTG GGATAGTTCA 
AAGCAAGCAG AAGCTACGTT GGGGAACTCA TTTGAGAGGA AGCTCGCGAT GCTGAAAGGT 
CTTTCACCAA AATTGGAAGA GTCGTCGCCG GACACCAGGA TCAGCCTGCG CAAGTCACCG 
TCGAGAAGAC CTGCAGCTGC AGGGAGACCA TCGGAGCCAG CAGCCACGCC GGAGCAGCAA 
CCGTCAGCAA GGAAGATTGC GTTCGGCTCT GCCGGGAGGT TGGCTAGAGA GAGATCCCCA 
TCAGCGTCCG GACTACTGTC CCCATCGTCA GCATCATCGT CTCCTAAGAG CCGCGTGAAC 
TCCTCGCCGT CGACACCGGC GGTGGCGCGG CGGCTCTTCA GCAGCTCCTC AATGCACGCG 
GCGGCCGAGG CAGCAGCAGC TGCCTCGCAT GTCGCCTCCC CTACGGCCAA AGGATCACGA 
CGACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898036.1 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753522.1 Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBr104085
Clone ID Related Accession (Same CDS sequence)	XM_014898036.1 , XM_014898036.2
Accession Version	XM_014898036.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA ATATAGTTCC AATTGCAAAA TCCTTCCATG GCAAGTTCTA TACGGGGAAC 
TGCTACATCA TACTGAATAC AGCTGAGCTT AAGAGCGGAA CCCGCCGGCA CAATGTGCAT 
TACTGGGTAG GGGAGGAAGC CAAACAGGAA GATTGCTTCA TGGCCTCGGA CAAGGCTGTC 
GAGTTGGATG CGGCTCTGGG CTCCCAAGCG GTCCAGTACA GGGAAACGCA AGGCGAAGAA 
TCCGACGAGT TCTTGTCATA CTTTAGACCA TGCATTATAC CAATACAAGG TAGCTTCTCT 
TCGCACTGGA GTAGATCTGG GGACGAATGT GACAGGACTA CGATGTTTCG GTGTGAAGGA 
GAGCATGTAC CTCGAGTCAG AGAGGTTCCA TTTTCGCGTT CTTCACTGGA TCATAGTGCA 
GCATTTATAG TGGACACGCC GTCGAAGATC TTTCTTTTCA GTGGTTGTAA TTCTAGTATA 
CAGACAAGGG CTAAGGCATT AGATGTCATT AAGCACCTGA GAGAAAACCG ACATTGCGGC 
AGATGTGAGA TTGGGACAAT AGAGGATGGA AAGCTTGTTG GTGATTCTGA TGCTGGTGAG 
TTCTGGAACC TATTTGGAGG CTATGCACCT ATTCCCCGTG ATGTACCAGG CACGGTCAAT 
GGGGAGTCGA TGACTACCTC ACCCAAGAAA CTTTTTTGGA TCAACAAAAG AAACCTTGTT 
CCCATGGAGG CAAATTTGTT AGACAGAGAA ATGCTGAACT CTGACAGGAG TTACATACTG 
GACTGTGGCA CTGAAATATT CTTGTGGATA GGGATAACAA CACTGGTTTC AGAGAGGAAG 
ACATCAATTA CCGCCTTAGA GGATTATGTA CATTGTCAAG GCAGATCATC AATTGGTCGT 
ACTGTTATAG TGACTGAAGG TCATGAAATT GCCGACTTCA AACTGCATTT TCAGCATTGG 
CCCAAGAACG TTGTGCAAAA GTTATATGAG GCAGGGCGTG AGAAAGTGGC AGCAATTTTC 
AAACATCAGG GCTACGATGT TGCAGAAATT CCGGAAGATA AATCGCCGCA GCTCATCAGT 
AGTAATGGTT GTCTAAAGGT TTGGCTGGTA GACCGCGGTT GTGCAACCCT CCTTTGCACT 
GAGGAGCAGG AGCAACTGTA CAATGGGGAC AGCTATATCA TACAGTATAG CTATGCTGAA 
GATGGAAAAG ATTATCATCT GTTCTTTGCT TGGTCTGGAC AGAATAGCAT ACAGGAAGAC 
AGAGTTGCTA CAGTTTCATT GGTGTCAAGC ATGGCCGATT CAGTCAAAGG GCATGCAGTT 
GTCGGGCAAA TGTTTGAGGG CAGAGAACCA GAGTTGTTTT TCCTAGTATT CAAGTCATTG 
GTTATATTCA AGGGGGGCAG GAGTGCTGCT GCTGCATATA TGAACTCTGT TCTACAGAAT 
AGCAATAGAA CTGAATGGTA TCAGAAAGAT GGAACTGCAT TATTTAGGGT TCAAGGGCTG 
AGACATGATT GCATACAAGC CATTCAGGTC AATCTGGCCA CAAATTCTCT CAATTCCTCG 
CACTGTTACA TTTTACAAGA CGGTGCTTCT TTGTTTACCT GGCTAGGAAG CCTTTCTTCA 
CCAGGTGATC ATGCTCTACT TGATAGGATG ATGAATAAGT TGTGGCCACT AAAGCAATCA 
TTGTTAGTAA GAGAAGGTTC TGAGCCTGAC CACTTCTGGA AAACATTAGG AGGAAGATCA 
GAATACTCTA AAGAAAAGCT TGTCAAGGGT TGGCCTGCAG ATCCACATAT GTATACTTGT 
ACTTTCGAAC AATGTCTGTT CAAGGCAAAG GAGGTGTTCA GCTTCACGCA GGATGATCTT 
GCAACTGAGG AGACTTTGAT TGTGGACTGC AGCGAAGAAA TCTACGTCTG GGTCGGACTT 
CACTCCGGTG TCACGTCCAA GGAGCATGCT CTTGACATCG GCAAGATGTT CCTTCAAGCA 
GGCATTGGTC AGGATGGACG GCGTTCAATC GACACAACTG TATATGCAGT TGCAGAAGGG 
GAGGAACCTG CATTTTTCAC CACCTTTTTC GACTGGGATA GTTCAAAGCA AGCAGAAGCT 
ACGTTGGGGA ACTCATTTGA GAGGAAGCTC GCGATGCTGA AAGGTCTTTC ACCAAAATTG 
GAAGAGTCGT CGCCGGACAC CAGGATCAGC CTGCGCAAGT CACCGTCGAG AAGACCTGCA 
GCTGCAGGGA GACCATCGGA GCCAGCAGCC ACGCCGGAGC AGCAACCGTC AGCAAGGAAG 
ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT AGAGAGAGAT CCCCATCAGC GTCCGGACTA 
CTGTCCCCAT CGTCAGCATC ATCGTCTCCT AAGAGCCGCG TGAACTCCTC GCCGTCGACA 
CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC TCCTCAATGC ACGCGGCGGC CGAGGCAGCA 
GCAGCTGCCT CGCATGTCGC CTCCCCTACG GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753522.1
CDS	192..2705
Translation	MGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898036.1
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898036.1

ORF Insert Sequence:

ATGGGCAGCA ATATAGTTCC AATTGCAAAA TCCTTCCATG GCAAGTTCTA TACGGGGAAC 
TGCTACATCA TACTGAATAC AGCTGAGCTT AAGAGCGGAA CCCGCCGGCA CAATGTGCAT 
TACTGGGTAG GGGAGGAAGC CAAACAGGAA GATTGCTTCA TGGCCTCGGA CAAGGCTGTC 
GAGTTGGATG CGGCTCTGGG CTCCCAAGCG GTCCAGTACA GGGAAACGCA AGGCGAAGAA 
TCCGACGAGT TCTTGTCATA CTTTAGACCA TGCATTATAC CAATACAAGG TAGCTTCTCT 
TCGCACTGGA GTAGATCTGG GGACGAATGT GACAGGACTA CGATGTTTCG GTGTGAAGGA 
GAGCATGTAC CTCGAGTCAG AGAGGTTCCA TTTTCGCGTT CTTCACTGGA TCATAGTGCA 
GCATTTATAG TGGACACGCC GTCGAAGATC TTTCTTTTCA GTGGTTGTAA TTCTAGTATA 
CAGACAAGGG CTAAGGCATT AGATGTCATT AAGCACCTGA GAGAAAACCG ACATTGCGGC 
AGATGTGAGA TTGGGACAAT AGAGGATGGA AAGCTTGTTG GTGATTCTGA TGCTGGTGAG 
TTCTGGAACC TATTTGGAGG CTATGCACCT ATTCCCCGTG ATGTACCAGG CACGGTCAAT 
GGGGAGTCGA TGACTACCTC ACCCAAGAAA CTTTTTTGGA TCAACAAAAG AAACCTTGTT 
CCCATGGAGG CAAATTTGTT AGACAGAGAA ATGCTGAACT CTGACAGGAG TTACATACTG 
GACTGTGGCA CTGAAATATT CTTGTGGATA GGGATAACAA CACTGGTTTC AGAGAGGAAG 
ACATCAATTA CCGCCTTAGA GGATTATGTA CATTGTCAAG GCAGATCATC AATTGGTCGT 
ACTGTTATAG TGACTGAAGG TCATGAAATT GCCGACTTCA AACTGCATTT TCAGCATTGG 
CCCAAGAACG TTGTGCAAAA GTTATATGAG GCAGGGCGTG AGAAAGTGGC AGCAATTTTC 
AAACATCAGG GCTACGATGT TGCAGAAATT CCGGAAGATA AATCGCCGCA GCTCATCAGT 
AGTAATGGTT GTCTAAAGGT TTGGCTGGTA GACCGCGGTT GTGCAACCCT CCTTTGCACT 
GAGGAGCAGG AGCAACTGTA CAATGGGGAC AGCTATATCA TACAGTATAG CTATGCTGAA 
GATGGAAAAG ATTATCATCT GTTCTTTGCT TGGTCTGGAC AGAATAGCAT ACAGGAAGAC 
AGAGTTGCTA CAGTTTCATT GGTGTCAAGC ATGGCCGATT CAGTCAAAGG GCATGCAGTT 
GTCGGGCAAA TGTTTGAGGG CAGAGAACCA GAGTTGTTTT TCCTAGTATT CAAGTCATTG 
GTTATATTCA AGGGGGGCAG GAGTGCTGCT GCTGCATATA TGAACTCTGT TCTACAGAAT 
AGCAATAGAA CTGAATGGTA TCAGAAAGAT GGAACTGCAT TATTTAGGGT TCAAGGGCTG 
AGACATGATT GCATACAAGC CATTCAGGTC AATCTGGCCA CAAATTCTCT CAATTCCTCG 
CACTGTTACA TTTTACAAGA CGGTGCTTCT TTGTTTACCT GGCTAGGAAG CCTTTCTTCA 
CCAGGTGATC ATGCTCTACT TGATAGGATG ATGAATAAGT TGTGGCCACT AAAGCAATCA 
TTGTTAGTAA GAGAAGGTTC TGAGCCTGAC CACTTCTGGA AAACATTAGG AGGAAGATCA 
GAATACTCTA AAGAAAAGCT TGTCAAGGGT TGGCCTGCAG ATCCACATAT GTATACTTGT 
ACTTTCGAAC AATGTCTGTT CAAGGCAAAG GAGGTGTTCA GCTTCACGCA GGATGATCTT 
GCAACTGAGG AGACTTTGAT TGTGGACTGC AGCGAAGAAA TCTACGTCTG GGTCGGACTT 
CACTCCGGTG TCACGTCCAA GGAGCATGCT CTTGACATCG GCAAGATGTT CCTTCAAGCA 
GGCATTGGTC AGGATGGACG GCGTTCAATC GACACAACTG TATATGCAGT TGCAGAAGGG 
GAGGAACCTG CATTTTTCAC CACCTTTTTC GACTGGGATA GTTCAAAGCA AGCAGAAGCT 
ACGTTGGGGA ACTCATTTGA GAGGAAGCTC GCGATGCTGA AAGGTCTTTC ACCAAAATTG 
GAAGAGTCGT CGCCGGACAC CAGGATCAGC CTGCGCAAGT CACCGTCGAG AAGACCTGCA 
GCTGCAGGGA GACCATCGGA GCCAGCAGCC ACGCCGGAGC AGCAACCGTC AGCAAGGAAG 
ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT AGAGAGAGAT CCCCATCAGC GTCCGGACTA 
CTGTCCCCAT CGTCAGCATC ATCGTCTCCT AAGAGCCGCG TGAACTCCTC GCCGTCGACA 
CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC TCCTCAATGC ACGCGGCGGC CGAGGCAGCA 
GCAGCTGCCT CGCATGTCGC CTCCCCTACG GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898036.2 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753522.1 Brachypodium distachyon villin-1 (LOC100826533), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBr104085
Clone ID Related Accession (Same CDS sequence)	XM_014898036.1 , XM_014898036.2
Accession Version	XM_014898036.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_014898036.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAGCA ATATAGTTCC AATTGCAAAA TCCTTCCATG GCAAGTTCTA TACGGGGAAC 
TGCTACATCA TACTGAATAC AGCTGAGCTT AAGAGCGGAA CCCGCCGGCA CAATGTGCAT 
TACTGGGTAG GGGAGGAAGC CAAACAGGAA GATTGCTTCA TGGCCTCGGA CAAGGCTGTC 
GAGTTGGATG CGGCTCTGGG CTCCCAAGCG GTCCAGTACA GGGAAACGCA AGGCGAAGAA 
TCCGACGAGT TCTTGTCATA CTTTAGACCA TGCATTATAC CAATACAAGG TAGCTTCTCT 
TCGCACTGGA GTAGATCTGG GGACGAATGT GACAGGACTA CGATGTTTCG GTGTGAAGGA 
GAGCATGTAC CTCGAGTCAG AGAGGTTCCA TTTTCGCGTT CTTCACTGGA TCATAGTGCA 
GCATTTATAG TGGACACGCC GTCGAAGATC TTTCTTTTCA GTGGTTGTAA TTCTAGTATA 
CAGACAAGGG CTAAGGCATT AGATGTCATT AAGCACCTGA GAGAAAACCG ACATTGCGGC 
AGATGTGAGA TTGGGACAAT AGAGGATGGA AAGCTTGTTG GTGATTCTGA TGCTGGTGAG 
TTCTGGAACC TATTTGGAGG CTATGCACCT ATTCCCCGTG ATGTACCAGG CACGGTCAAT 
GGGGAGTCGA TGACTACCTC ACCCAAGAAA CTTTTTTGGA TCAACAAAAG AAACCTTGTT 
CCCATGGAGG CAAATTTGTT AGACAGAGAA ATGCTGAACT CTGACAGGAG TTACATACTG 
GACTGTGGCA CTGAAATATT CTTGTGGATA GGGATAACAA CACTGGTTTC AGAGAGGAAG 
ACATCAATTA CCGCCTTAGA GGATTATGTA CATTGTCAAG GCAGATCATC AATTGGTCGT 
ACTGTTATAG TGACTGAAGG TCATGAAATT GCCGACTTCA AACTGCATTT TCAGCATTGG 
CCCAAGAACG TTGTGCAAAA GTTATATGAG GCAGGGCGTG AGAAAGTGGC AGCAATTTTC 
AAACATCAGG GCTACGATGT TGCAGAAATT CCGGAAGATA AATCGCCGCA GCTCATCAGT 
AGTAATGGTT GTCTAAAGGT TTGGCTGGTA GACCGCGGTT GTGCAACCCT CCTTTGCACT 
GAGGAGCAGG AGCAACTGTA CAATGGGGAC AGCTATATCA TACAGTATAG CTATGCTGAA 
GATGGAAAAG ATTATCATCT GTTCTTTGCT TGGTCTGGAC AGAATAGCAT ACAGGAAGAC 
AGAGTTGCTA CAGTTTCATT GGTGTCAAGC ATGGCCGATT CAGTCAAAGG GCATGCAGTT 
GTCGGGCAAA TGTTTGAGGG CAGAGAACCA GAGTTGTTTT TCCTAGTATT CAAGTCATTG 
GTTATATTCA AGGGGGGCAG GAGTGCTGCT GCTGCATATA TGAACTCTGT TCTACAGAAT 
AGCAATAGAA CTGAATGGTA TCAGAAAGAT GGAACTGCAT TATTTAGGGT TCAAGGGCTG 
AGACATGATT GCATACAAGC CATTCAGGTC AATCTGGCCA CAAATTCTCT CAATTCCTCG 
CACTGTTACA TTTTACAAGA CGGTGCTTCT TTGTTTACCT GGCTAGGAAG CCTTTCTTCA 
CCAGGTGATC ATGCTCTACT TGATAGGATG ATGAATAAGT TGTGGCCACT AAAGCAATCA 
TTGTTAGTAA GAGAAGGTTC TGAGCCTGAC CACTTCTGGA AAACATTAGG AGGAAGATCA 
GAATACTCTA AAGAAAAGCT TGTCAAGGGT TGGCCTGCAG ATCCACATAT GTATACTTGT 
ACTTTCGAAC AATGTCTGTT CAAGGCAAAG GAGGTGTTCA GCTTCACGCA GGATGATCTT 
GCAACTGAGG AGACTTTGAT TGTGGACTGC AGCGAAGAAA TCTACGTCTG GGTCGGACTT 
CACTCCGGTG TCACGTCCAA GGAGCATGCT CTTGACATCG GCAAGATGTT CCTTCAAGCA 
GGCATTGGTC AGGATGGACG GCGTTCAATC GACACAACTG TATATGCAGT TGCAGAAGGG 
GAGGAACCTG CATTTTTCAC CACCTTTTTC GACTGGGATA GTTCAAAGCA AGCAGAAGCT 
ACGTTGGGGA ACTCATTTGA GAGGAAGCTC GCGATGCTGA AAGGTCTTTC ACCAAAATTG 
GAAGAGTCGT CGCCGGACAC CAGGATCAGC CTGCGCAAGT CACCGTCGAG AAGACCTGCA 
GCTGCAGGGA GACCATCGGA GCCAGCAGCC ACGCCGGAGC AGCAACCGTC AGCAAGGAAG 
ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT AGAGAGAGAT CCCCATCAGC GTCCGGACTA 
CTGTCCCCAT CGTCAGCATC ATCGTCTCCT AAGAGCCGCG TGAACTCCTC GCCGTCGACA 
CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC TCCTCAATGC ACGCGGCGGC CGAGGCAGCA 
GCAGCTGCCT CGCATGTCGC CTCCCCTACG GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753522.1
CDS	278..2791
Translation	MGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898036.2
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898036.2

ORF Insert Sequence:

ATGGGCAGCA ATATAGTTCC AATTGCAAAA TCCTTCCATG GCAAGTTCTA TACGGGGAAC 
TGCTACATCA TACTGAATAC AGCTGAGCTT AAGAGCGGAA CCCGCCGGCA CAATGTGCAT 
TACTGGGTAG GGGAGGAAGC CAAACAGGAA GATTGCTTCA TGGCCTCGGA CAAGGCTGTC 
GAGTTGGATG CGGCTCTGGG CTCCCAAGCG GTCCAGTACA GGGAAACGCA AGGCGAAGAA 
TCCGACGAGT TCTTGTCATA CTTTAGACCA TGCATTATAC CAATACAAGG TAGCTTCTCT 
TCGCACTGGA GTAGATCTGG GGACGAATGT GACAGGACTA CGATGTTTCG GTGTGAAGGA 
GAGCATGTAC CTCGAGTCAG AGAGGTTCCA TTTTCGCGTT CTTCACTGGA TCATAGTGCA 
GCATTTATAG TGGACACGCC GTCGAAGATC TTTCTTTTCA GTGGTTGTAA TTCTAGTATA 
CAGACAAGGG CTAAGGCATT AGATGTCATT AAGCACCTGA GAGAAAACCG ACATTGCGGC 
AGATGTGAGA TTGGGACAAT AGAGGATGGA AAGCTTGTTG GTGATTCTGA TGCTGGTGAG 
TTCTGGAACC TATTTGGAGG CTATGCACCT ATTCCCCGTG ATGTACCAGG CACGGTCAAT 
GGGGAGTCGA TGACTACCTC ACCCAAGAAA CTTTTTTGGA TCAACAAAAG AAACCTTGTT 
CCCATGGAGG CAAATTTGTT AGACAGAGAA ATGCTGAACT CTGACAGGAG TTACATACTG 
GACTGTGGCA CTGAAATATT CTTGTGGATA GGGATAACAA CACTGGTTTC AGAGAGGAAG 
ACATCAATTA CCGCCTTAGA GGATTATGTA CATTGTCAAG GCAGATCATC AATTGGTCGT 
ACTGTTATAG TGACTGAAGG TCATGAAATT GCCGACTTCA AACTGCATTT TCAGCATTGG 
CCCAAGAACG TTGTGCAAAA GTTATATGAG GCAGGGCGTG AGAAAGTGGC AGCAATTTTC 
AAACATCAGG GCTACGATGT TGCAGAAATT CCGGAAGATA AATCGCCGCA GCTCATCAGT 
AGTAATGGTT GTCTAAAGGT TTGGCTGGTA GACCGCGGTT GTGCAACCCT CCTTTGCACT 
GAGGAGCAGG AGCAACTGTA CAATGGGGAC AGCTATATCA TACAGTATAG CTATGCTGAA 
GATGGAAAAG ATTATCATCT GTTCTTTGCT TGGTCTGGAC AGAATAGCAT ACAGGAAGAC 
AGAGTTGCTA CAGTTTCATT GGTGTCAAGC ATGGCCGATT CAGTCAAAGG GCATGCAGTT 
GTCGGGCAAA TGTTTGAGGG CAGAGAACCA GAGTTGTTTT TCCTAGTATT CAAGTCATTG 
GTTATATTCA AGGGGGGCAG GAGTGCTGCT GCTGCATATA TGAACTCTGT TCTACAGAAT 
AGCAATAGAA CTGAATGGTA TCAGAAAGAT GGAACTGCAT TATTTAGGGT TCAAGGGCTG 
AGACATGATT GCATACAAGC CATTCAGGTC AATCTGGCCA CAAATTCTCT CAATTCCTCG 
CACTGTTACA TTTTACAAGA CGGTGCTTCT TTGTTTACCT GGCTAGGAAG CCTTTCTTCA 
CCAGGTGATC ATGCTCTACT TGATAGGATG ATGAATAAGT TGTGGCCACT AAAGCAATCA 
TTGTTAGTAA GAGAAGGTTC TGAGCCTGAC CACTTCTGGA AAACATTAGG AGGAAGATCA 
GAATACTCTA AAGAAAAGCT TGTCAAGGGT TGGCCTGCAG ATCCACATAT GTATACTTGT 
ACTTTCGAAC AATGTCTGTT CAAGGCAAAG GAGGTGTTCA GCTTCACGCA GGATGATCTT 
GCAACTGAGG AGACTTTGAT TGTGGACTGC AGCGAAGAAA TCTACGTCTG GGTCGGACTT 
CACTCCGGTG TCACGTCCAA GGAGCATGCT CTTGACATCG GCAAGATGTT CCTTCAAGCA 
GGCATTGGTC AGGATGGACG GCGTTCAATC GACACAACTG TATATGCAGT TGCAGAAGGG 
GAGGAACCTG CATTTTTCAC CACCTTTTTC GACTGGGATA GTTCAAAGCA AGCAGAAGCT 
ACGTTGGGGA ACTCATTTGA GAGGAAGCTC GCGATGCTGA AAGGTCTTTC ACCAAAATTG 
GAAGAGTCGT CGCCGGACAC CAGGATCAGC CTGCGCAAGT CACCGTCGAG AAGACCTGCA 
GCTGCAGGGA GACCATCGGA GCCAGCAGCC ACGCCGGAGC AGCAACCGTC AGCAAGGAAG 
ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT AGAGAGAGAT CCCCATCAGC GTCCGGACTA 
CTGTCCCCAT CGTCAGCATC ATCGTCTCCT AAGAGCCGCG TGAACTCCTC GCCGTCGACA 
CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC TCCTCAATGC ACGCGGCGGC CGAGGCAGCA 
GCAGCTGCCT CGCATGTCGC CTCCCCTACG GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898037.1 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753523.1 Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBr104086
Clone ID Related Accession (Same CDS sequence)	XM_014898037.1 , XM_014898037.2
Accession Version	XM_014898037.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG AGGAAGATCA GAATACTCTA AAGAAAAGCT TGTCAAGGGT 
TGGCCTGCAG ATCCACATAT GTATACTTGT ACTTTCGAAC AATGTCTGTT CAAGGCAAAG 
GAGGTGTTCA GCTTCACGCA GGATGATCTT GCAACTGAGG AGACTTTGAT TGTGGACTGC 
AGCGAAGAAA TCTACGTCTG GGTCGGACTT CACTCCGGTG TCACGTCCAA GGAGCATGCT 
CTTGACATCG GCAAGATGTT CCTTCAAGCA GGCATTGGTC AGGATGGACG GCGTTCAATC 
GACACAACTG TATATGCAGT TGCAGAAGGG GAGGAACCTG CATTTTTCAC CACCTTTTTC 
GACTGGGATA GTTCAAAGCA AGCAGAAGCT ACGTTGGGGA ACTCATTTGA GAGGAAGCTC 
GCGATGCTGA AAGGTCTTTC ACCAAAATTG GAAGAGTCGT CGCCGGACAC CAGGATCAGC 
CTGCGCAAGT CACCGTCGAG AAGACCTGCA GCTGCAGGGA GACCATCGGA GCCAGCAGCC 
ACGCCGGAGC AGCAACCGTC AGCAAGGAAG ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT 
AGAGAGAGAT CCCCATCAGC GTCCGGACTA CTGTCCCCAT CGTCAGCATC ATCGTCTCCT 
AAGAGCCGCG TGAACTCCTC GCCGTCGACA CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC 
TCCTCAATGC ACGCGGCGGC CGAGGCAGCA GCAGCTGCCT CGCATGTCGC CTCCCCTACG 
GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753523.1
CDS	107..2590
Translation	MATLKGVDDAFLGVGDKPGLDIWCVMGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898037.1
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898037.1

ORF Insert Sequence:

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG AGGAAGATCA GAATACTCTA AAGAAAAGCT TGTCAAGGGT 
TGGCCTGCAG ATCCACATAT GTATACTTGT ACTTTCGAAC AATGTCTGTT CAAGGCAAAG 
GAGGTGTTCA GCTTCACGCA GGATGATCTT GCAACTGAGG AGACTTTGAT TGTGGACTGC 
AGCGAAGAAA TCTACGTCTG GGTCGGACTT CACTCCGGTG TCACGTCCAA GGAGCATGCT 
CTTGACATCG GCAAGATGTT CCTTCAAGCA GGCATTGGTC AGGATGGACG GCGTTCAATC 
GACACAACTG TATATGCAGT TGCAGAAGGG GAGGAACCTG CATTTTTCAC CACCTTTTTC 
GACTGGGATA GTTCAAAGCA AGCAGAAGCT ACGTTGGGGA ACTCATTTGA GAGGAAGCTC 
GCGATGCTGA AAGGTCTTTC ACCAAAATTG GAAGAGTCGT CGCCGGACAC CAGGATCAGC 
CTGCGCAAGT CACCGTCGAG AAGACCTGCA GCTGCAGGGA GACCATCGGA GCCAGCAGCC 
ACGCCGGAGC AGCAACCGTC AGCAAGGAAG ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT 
AGAGAGAGAT CCCCATCAGC GTCCGGACTA CTGTCCCCAT CGTCAGCATC ATCGTCTCCT 
AAGAGCCGCG TGAACTCCTC GCCGTCGACA CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC 
TCCTCAATGC ACGCGGCGGC CGAGGCAGCA GCAGCTGCCT CGCATGTCGC CTCCCCTACG 
GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898037.2 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753523.1 Brachypodium distachyon villin-1 (LOC100826533), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBr104086
Clone ID Related Accession (Same CDS sequence)	XM_014898037.1 , XM_014898037.2
Accession Version	XM_014898037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_014898037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG AGGAAGATCA GAATACTCTA AAGAAAAGCT TGTCAAGGGT 
TGGCCTGCAG ATCCACATAT GTATACTTGT ACTTTCGAAC AATGTCTGTT CAAGGCAAAG 
GAGGTGTTCA GCTTCACGCA GGATGATCTT GCAACTGAGG AGACTTTGAT TGTGGACTGC 
AGCGAAGAAA TCTACGTCTG GGTCGGACTT CACTCCGGTG TCACGTCCAA GGAGCATGCT 
CTTGACATCG GCAAGATGTT CCTTCAAGCA GGCATTGGTC AGGATGGACG GCGTTCAATC 
GACACAACTG TATATGCAGT TGCAGAAGGG GAGGAACCTG CATTTTTCAC CACCTTTTTC 
GACTGGGATA GTTCAAAGCA AGCAGAAGCT ACGTTGGGGA ACTCATTTGA GAGGAAGCTC 
GCGATGCTGA AAGGTCTTTC ACCAAAATTG GAAGAGTCGT CGCCGGACAC CAGGATCAGC 
CTGCGCAAGT CACCGTCGAG AAGACCTGCA GCTGCAGGGA GACCATCGGA GCCAGCAGCC 
ACGCCGGAGC AGCAACCGTC AGCAAGGAAG ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT 
AGAGAGAGAT CCCCATCAGC GTCCGGACTA CTGTCCCCAT CGTCAGCATC ATCGTCTCCT 
AAGAGCCGCG TGAACTCCTC GCCGTCGACA CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC 
TCCTCAATGC ACGCGGCGGC CGAGGCAGCA GCAGCTGCCT CGCATGTCGC CTCCCCTACG 
GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753523.1
CDS	163..2646
Translation	MATLKGVDDAFLGVGDKPGLDIWCVMGSNIVPIAKSFHGKFYTGNCYIILNTAELKSGTRRHNVHYWVGEEAKQEDCFMASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898037.2
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898037.2

ORF Insert Sequence:

ATGGCGACCC TCAAGGGCGT CGATGACGCC TTCCTCGGCG TCGGGGACAA GCCAGGGTTG 
GACATCTGGT GCGTTATGGG CAGCAATATA GTTCCAATTG CAAAATCCTT CCATGGCAAG 
TTCTATACGG GGAACTGCTA CATCATACTG AATACAGCTG AGCTTAAGAG CGGAACCCGC 
CGGCACAATG TGCATTACTG GGTAGGGGAG GAAGCCAAAC AGGAAGATTG CTTCATGGCC 
TCGGACAAGG CTGTCGAGTT GGATGCGGCT CTGGGCTCCC AAGCGGTCCA GTACAGGGAA 
ACGCAAGGCG AAGAATCCGA CGAGTTCTTG TCATACTTTA GACCATGCAT TATACCAATA 
CAAGGTAGCT TCTCTTCGCA CTGGAGTAGA TCTGGGGACG AATGTGACAG GACTACGATG 
TTTCGGTGTG AAGGAGAGCA TGTACCTCGA GTCAGAGAGG TTCCATTTTC GCGTTCTTCA 
CTGGATCATA GTGCAGCATT TATAGTGGAC ACGCCGTCGA AGATCTTTCT TTTCAGTGGT 
TGTAATTCTA GTATACAGAC AAGGGCTAAG GCATTAGATG TCATTAAGCA CCTGAGAGAA 
AACCGACATT GCGGCAGATG TGAGATTGGG ACAATAGAGG ATGGAAAGCT TGTTGGTGAT 
TCTGATGCTG GTGAGTTCTG GAACCTATTT GGAGGCTATG CACCTATTCC CCGTGATGTA 
CCAGGCACGG TCAATGGGGA GTCGATGACT ACCTCACCCA AGAAACTTTT TTGGATCAAC 
AAAAGAAACC TTGTTCCCAT GGAGGCAAAT TTGTTAGACA GAGAAATGCT GAACTCTGAC 
AGGAGTTACA TACTGGACTG TGGCACTGAA ATATTCTTGT GGATAGGGAT AACAACACTG 
GTTTCAGAGA GGAAGACATC AATTACCGCC TTAGAGGATT ATGTACATTG TCAAGGCAGA 
TCATCAATTG GTCGTACTGT TATAGTGACT GAAGGTCATG AAATTGCCGA CTTCAAACTG 
CATTTTCAGC ATTGGCCCAA GAACGTTGTG CAAAAGTTAT ATGAGGCAGG GCGTGAGAAA 
GTGGCAGCAA TTTTCAAACA TCAGGGCTAC GATGTTGCAG AAATTCCGGA AGATAAATCG 
CCGCAGCTCA TCAGTAGTAA TGGTTGTCTA AAGGTTTGGC TGGTAGACCG CGGTTGTGCA 
ACCCTCCTTT GCACTGAGGA GCAGGAGCAA CTGTACAATG GGGACAGCTA TATCATACAG 
TATAGCTATG CTGAAGATGG AAAAGATTAT CATCTGTTCT TTGCTTGGTC TGGACAGAAT 
AGCATACAGG AAGACAGAGT TGCTACAGTT TCATTGGTGT CAAGCATGGC CGATTCAGTC 
AAAGGGCATG CAGTTGTCGG GCAAATGTTT GAGGGCAGAG AACCAGAGTT GTTTTTCCTA 
GTATTCAAGT CATTGGTTAT ATTCAAGGGG GGCAGGAGTG CTGCTGCTGC ATATATGAAC 
TCTGTTCTAC AGAATAGCAA TAGAACTGAA TGGTATCAGA AAGATGGAAC TGCATTATTT 
AGGGTTCAAG GGCTGAGACA TGATTGCATA CAAGCCATTC AGGTCAATCT GGCCACAAAT 
TCTCTCAATT CCTCGCACTG TTACATTTTA CAAGACGGTG CTTCTTTGTT TACCTGGCTA 
GGAAGCCTTT CTTCACCAGG AGGAAGATCA GAATACTCTA AAGAAAAGCT TGTCAAGGGT 
TGGCCTGCAG ATCCACATAT GTATACTTGT ACTTTCGAAC AATGTCTGTT CAAGGCAAAG 
GAGGTGTTCA GCTTCACGCA GGATGATCTT GCAACTGAGG AGACTTTGAT TGTGGACTGC 
AGCGAAGAAA TCTACGTCTG GGTCGGACTT CACTCCGGTG TCACGTCCAA GGAGCATGCT 
CTTGACATCG GCAAGATGTT CCTTCAAGCA GGCATTGGTC AGGATGGACG GCGTTCAATC 
GACACAACTG TATATGCAGT TGCAGAAGGG GAGGAACCTG CATTTTTCAC CACCTTTTTC 
GACTGGGATA GTTCAAAGCA AGCAGAAGCT ACGTTGGGGA ACTCATTTGA GAGGAAGCTC 
GCGATGCTGA AAGGTCTTTC ACCAAAATTG GAAGAGTCGT CGCCGGACAC CAGGATCAGC 
CTGCGCAAGT CACCGTCGAG AAGACCTGCA GCTGCAGGGA GACCATCGGA GCCAGCAGCC 
ACGCCGGAGC AGCAACCGTC AGCAAGGAAG ATTGCGTTCG GCTCTGCCGG GAGGTTGGCT 
AGAGAGAGAT CCCCATCAGC GTCCGGACTA CTGTCCCCAT CGTCAGCATC ATCGTCTCCT 
AAGAGCCGCG TGAACTCCTC GCCGTCGACA CCGGCGGTGG CGCGGCGGCT CTTCAGCAGC 
TCCTCAATGC ACGCGGCGGC CGAGGCAGCA GCAGCTGCCT CGCATGTCGC CTCCCCTACG 
GCCAAAGGAT CACGACGACG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898038.2 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753524.1 Brachypodium distachyon villin-1 (LOC100826533), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBr104087
Clone ID Related Accession (Same CDS sequence)	XM_014898038.2 , XM_014898038.1
Accession Version	XM_014898038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_014898038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG ACAAGGCTGT CGAGTTGGAT GCGGCTCTGG GCTCCCAAGC GGTCCAGTAC 
AGGGAAACGC AAGGCGAAGA ATCCGACGAG TTCTTGTCAT ACTTTAGACC ATGCATTATA 
CCAATACAAG GTAGCTTCTC TTCGCACTGG AGTAGATCTG GGGACGAATG TGACAGGACT 
ACGATGTTTC GGTGTGAAGG AGAGCATGTA CCTCGAGTCA GAGAGGTTCC ATTTTCGCGT 
TCTTCACTGG ATCATAGTGC AGCATTTATA GTGGACACGC CGTCGAAGAT CTTTCTTTTC 
AGTGGTTGTA ATTCTAGTAT ACAGACAAGG GCTAAGGCAT TAGATGTCAT TAAGCACCTG 
AGAGAAAACC GACATTGCGG CAGATGTGAG ATTGGGACAA TAGAGGATGG AAAGCTTGTT 
GGTGATTCTG ATGCTGGTGA GTTCTGGAAC CTATTTGGAG GCTATGCACC TATTCCCCGT 
GATGTACCAG GCACGGTCAA TGGGGAGTCG ATGACTACCT CACCCAAGAA ACTTTTTTGG 
ATCAACAAAA GAAACCTTGT TCCCATGGAG GCAAATTTGT TAGACAGAGA AATGCTGAAC 
TCTGACAGGA GTTACATACT GGACTGTGGC ACTGAAATAT TCTTGTGGAT AGGGATAACA 
ACACTGGTTT CAGAGAGGAA GACATCAATT ACCGCCTTAG AGGATTATGT ACATTGTCAA 
GGCAGATCAT CAATTGGTCG TACTGTTATA GTGACTGAAG GTCATGAAAT TGCCGACTTC 
AAACTGCATT TTCAGCATTG GCCCAAGAAC GTTGTGCAAA AGTTATATGA GGCAGGGCGT 
GAGAAAGTGG CAGCAATTTT CAAACATCAG GGCTACGATG TTGCAGAAAT TCCGGAAGAT 
AAATCGCCGC AGCTCATCAG TAGTAATGGT TGTCTAAAGG TTTGGCTGGT AGACCGCGGT 
TGTGCAACCC TCCTTTGCAC TGAGGAGCAG GAGCAACTGT ACAATGGGGA CAGCTATATC 
ATACAGTATA GCTATGCTGA AGATGGAAAA GATTATCATC TGTTCTTTGC TTGGTCTGGA 
CAGAATAGCA TACAGGAAGA CAGAGTTGCT ACAGTTTCAT TGGTGTCAAG CATGGCCGAT 
TCAGTCAAAG GGCATGCAGT TGTCGGGCAA ATGTTTGAGG GCAGAGAACC AGAGTTGTTT 
TTCCTAGTAT TCAAGTCATT GGTTATATTC AAGGGGGGCA GGAGTGCTGC TGCTGCATAT 
ATGAACTCTG TTCTACAGAA TAGCAATAGA ACTGAATGGT ATCAGAAAGA TGGAACTGCA 
TTATTTAGGG TTCAAGGGCT GAGACATGAT TGCATACAAG CCATTCAGGT CAATCTGGCC 
ACAAATTCTC TCAATTCCTC GCACTGTTAC ATTTTACAAG ACGGTGCTTC TTTGTTTACC 
TGGCTAGGAA GCCTTTCTTC ACCAGGTGAT CATGCTCTAC TTGATAGGAT GATGAATAAG 
TTGTGGCCAC TAAAGCAATC ATTGTTAGTA AGAGAAGGTT CTGAGCCTGA CCACTTCTGG 
AAAACATTAG GAGGAAGATC AGAATACTCT AAAGAAAAGC TTGTCAAGGG TTGGCCTGCA 
GATCCACATA TGTATACTTG TACTTTCGAA CAATGTCTGT TCAAGGCAAA GGAGGTGTTC 
AGCTTCACGC AGGATGATCT TGCAACTGAG GAGACTTTGA TTGTGGACTG CAGCGAAGAA 
ATCTACGTCT GGGTCGGACT TCACTCCGGT GTCACGTCCA AGGAGCATGC TCTTGACATC 
GGCAAGATGT TCCTTCAAGC AGGCATTGGT CAGGATGGAC GGCGTTCAAT CGACACAACT 
GTATATGCAG TTGCAGAAGG GGAGGAACCT GCATTTTTCA CCACCTTTTT CGACTGGGAT 
AGTTCAAAGC AAGCAGAAGC TACGTTGGGG AACTCATTTG AGAGGAAGCT CGCGATGCTG 
AAAGGTCTTT CACCAAAATT GGAAGAGTCG TCGCCGGACA CCAGGATCAG CCTGCGCAAG 
TCACCGTCGA GAAGACCTGC AGCTGCAGGG AGACCATCGG AGCCAGCAGC CACGCCGGAG 
CAGCAACCGT CAGCAAGGAA GATTGCGTTC GGCTCTGCCG GGAGGTTGGC TAGAGAGAGA 
TCCCCATCAG CGTCCGGACT ACTGTCCCCA TCGTCAGCAT CATCGTCTCC TAAGAGCCGC 
GTGAACTCCT CGCCGTCGAC ACCGGCGGTG GCGCGGCGGC TCTTCAGCAG CTCCTCAATG 
CACGCGGCGG CCGAGGCAGC AGCAGCTGCC TCGCATGTCG CCTCCCCTAC GGCCAAAGGA 
TCACGACGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753524.1
CDS	95..2449
Translation	MASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898038.2
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1 (LOC100826533), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898038.2

ORF Insert Sequence:

ATGGCCTCGG ACAAGGCTGT CGAGTTGGAT GCGGCTCTGG GCTCCCAAGC GGTCCAGTAC 
AGGGAAACGC AAGGCGAAGA ATCCGACGAG TTCTTGTCAT ACTTTAGACC ATGCATTATA 
CCAATACAAG GTAGCTTCTC TTCGCACTGG AGTAGATCTG GGGACGAATG TGACAGGACT 
ACGATGTTTC GGTGTGAAGG AGAGCATGTA CCTCGAGTCA GAGAGGTTCC ATTTTCGCGT 
TCTTCACTGG ATCATAGTGC AGCATTTATA GTGGACACGC CGTCGAAGAT CTTTCTTTTC 
AGTGGTTGTA ATTCTAGTAT ACAGACAAGG GCTAAGGCAT TAGATGTCAT TAAGCACCTG 
AGAGAAAACC GACATTGCGG CAGATGTGAG ATTGGGACAA TAGAGGATGG AAAGCTTGTT 
GGTGATTCTG ATGCTGGTGA GTTCTGGAAC CTATTTGGAG GCTATGCACC TATTCCCCGT 
GATGTACCAG GCACGGTCAA TGGGGAGTCG ATGACTACCT CACCCAAGAA ACTTTTTTGG 
ATCAACAAAA GAAACCTTGT TCCCATGGAG GCAAATTTGT TAGACAGAGA AATGCTGAAC 
TCTGACAGGA GTTACATACT GGACTGTGGC ACTGAAATAT TCTTGTGGAT AGGGATAACA 
ACACTGGTTT CAGAGAGGAA GACATCAATT ACCGCCTTAG AGGATTATGT ACATTGTCAA 
GGCAGATCAT CAATTGGTCG TACTGTTATA GTGACTGAAG GTCATGAAAT TGCCGACTTC 
AAACTGCATT TTCAGCATTG GCCCAAGAAC GTTGTGCAAA AGTTATATGA GGCAGGGCGT 
GAGAAAGTGG CAGCAATTTT CAAACATCAG GGCTACGATG TTGCAGAAAT TCCGGAAGAT 
AAATCGCCGC AGCTCATCAG TAGTAATGGT TGTCTAAAGG TTTGGCTGGT AGACCGCGGT 
TGTGCAACCC TCCTTTGCAC TGAGGAGCAG GAGCAACTGT ACAATGGGGA CAGCTATATC 
ATACAGTATA GCTATGCTGA AGATGGAAAA GATTATCATC TGTTCTTTGC TTGGTCTGGA 
CAGAATAGCA TACAGGAAGA CAGAGTTGCT ACAGTTTCAT TGGTGTCAAG CATGGCCGAT 
TCAGTCAAAG GGCATGCAGT TGTCGGGCAA ATGTTTGAGG GCAGAGAACC AGAGTTGTTT 
TTCCTAGTAT TCAAGTCATT GGTTATATTC AAGGGGGGCA GGAGTGCTGC TGCTGCATAT 
ATGAACTCTG TTCTACAGAA TAGCAATAGA ACTGAATGGT ATCAGAAAGA TGGAACTGCA 
TTATTTAGGG TTCAAGGGCT GAGACATGAT TGCATACAAG CCATTCAGGT CAATCTGGCC 
ACAAATTCTC TCAATTCCTC GCACTGTTAC ATTTTACAAG ACGGTGCTTC TTTGTTTACC 
TGGCTAGGAA GCCTTTCTTC ACCAGGTGAT CATGCTCTAC TTGATAGGAT GATGAATAAG 
TTGTGGCCAC TAAAGCAATC ATTGTTAGTA AGAGAAGGTT CTGAGCCTGA CCACTTCTGG 
AAAACATTAG GAGGAAGATC AGAATACTCT AAAGAAAAGC TTGTCAAGGG TTGGCCTGCA 
GATCCACATA TGTATACTTG TACTTTCGAA CAATGTCTGT TCAAGGCAAA GGAGGTGTTC 
AGCTTCACGC AGGATGATCT TGCAACTGAG GAGACTTTGA TTGTGGACTG CAGCGAAGAA 
ATCTACGTCT GGGTCGGACT TCACTCCGGT GTCACGTCCA AGGAGCATGC TCTTGACATC 
GGCAAGATGT TCCTTCAAGC AGGCATTGGT CAGGATGGAC GGCGTTCAAT CGACACAACT 
GTATATGCAG TTGCAGAAGG GGAGGAACCT GCATTTTTCA CCACCTTTTT CGACTGGGAT 
AGTTCAAAGC AAGCAGAAGC TACGTTGGGG AACTCATTTG AGAGGAAGCT CGCGATGCTG 
AAAGGTCTTT CACCAAAATT GGAAGAGTCG TCGCCGGACA CCAGGATCAG CCTGCGCAAG 
TCACCGTCGA GAAGACCTGC AGCTGCAGGG AGACCATCGG AGCCAGCAGC CACGCCGGAG 
CAGCAACCGT CAGCAAGGAA GATTGCGTTC GGCTCTGCCG GGAGGTTGGC TAGAGAGAGA 
TCCCCATCAG CGTCCGGACT ACTGTCCCCA TCGTCAGCAT CATCGTCTCC TAAGAGCCGC 
GTGAACTCCT CGCCGTCGAC ACCGGCGGTG GCGCGGCGGC TCTTCAGCAG CTCCTCAATG 
CACGCGGCGG CCGAGGCAGC AGCAGCTGCC TCGCATGTCG CCTCCCCTAC GGCCAAAGGA 
TCACGACGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100826533 ( XM_014898038.1 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_014753524.1 Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X4, mRNA.

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CloneID	OBr104087
Clone ID Related Accession (Same CDS sequence)	XM_014898038.2 , XM_014898038.1
Accession Version	XM_014898038.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	villin-1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG ACAAGGCTGT CGAGTTGGAT GCGGCTCTGG GCTCCCAAGC GGTCCAGTAC 
AGGGAAACGC AAGGCGAAGA ATCCGACGAG TTCTTGTCAT ACTTTAGACC ATGCATTATA 
CCAATACAAG GTAGCTTCTC TTCGCACTGG AGTAGATCTG GGGACGAATG TGACAGGACT 
ACGATGTTTC GGTGTGAAGG AGAGCATGTA CCTCGAGTCA GAGAGGTTCC ATTTTCGCGT 
TCTTCACTGG ATCATAGTGC AGCATTTATA GTGGACACGC CGTCGAAGAT CTTTCTTTTC 
AGTGGTTGTA ATTCTAGTAT ACAGACAAGG GCTAAGGCAT TAGATGTCAT TAAGCACCTG 
AGAGAAAACC GACATTGCGG CAGATGTGAG ATTGGGACAA TAGAGGATGG AAAGCTTGTT 
GGTGATTCTG ATGCTGGTGA GTTCTGGAAC CTATTTGGAG GCTATGCACC TATTCCCCGT 
GATGTACCAG GCACGGTCAA TGGGGAGTCG ATGACTACCT CACCCAAGAA ACTTTTTTGG 
ATCAACAAAA GAAACCTTGT TCCCATGGAG GCAAATTTGT TAGACAGAGA AATGCTGAAC 
TCTGACAGGA GTTACATACT GGACTGTGGC ACTGAAATAT TCTTGTGGAT AGGGATAACA 
ACACTGGTTT CAGAGAGGAA GACATCAATT ACCGCCTTAG AGGATTATGT ACATTGTCAA 
GGCAGATCAT CAATTGGTCG TACTGTTATA GTGACTGAAG GTCATGAAAT TGCCGACTTC 
AAACTGCATT TTCAGCATTG GCCCAAGAAC GTTGTGCAAA AGTTATATGA GGCAGGGCGT 
GAGAAAGTGG CAGCAATTTT CAAACATCAG GGCTACGATG TTGCAGAAAT TCCGGAAGAT 
AAATCGCCGC AGCTCATCAG TAGTAATGGT TGTCTAAAGG TTTGGCTGGT AGACCGCGGT 
TGTGCAACCC TCCTTTGCAC TGAGGAGCAG GAGCAACTGT ACAATGGGGA CAGCTATATC 
ATACAGTATA GCTATGCTGA AGATGGAAAA GATTATCATC TGTTCTTTGC TTGGTCTGGA 
CAGAATAGCA TACAGGAAGA CAGAGTTGCT ACAGTTTCAT TGGTGTCAAG CATGGCCGAT 
TCAGTCAAAG GGCATGCAGT TGTCGGGCAA ATGTTTGAGG GCAGAGAACC AGAGTTGTTT 
TTCCTAGTAT TCAAGTCATT GGTTATATTC AAGGGGGGCA GGAGTGCTGC TGCTGCATAT 
ATGAACTCTG TTCTACAGAA TAGCAATAGA ACTGAATGGT ATCAGAAAGA TGGAACTGCA 
TTATTTAGGG TTCAAGGGCT GAGACATGAT TGCATACAAG CCATTCAGGT CAATCTGGCC 
ACAAATTCTC TCAATTCCTC GCACTGTTAC ATTTTACAAG ACGGTGCTTC TTTGTTTACC 
TGGCTAGGAA GCCTTTCTTC ACCAGGTGAT CATGCTCTAC TTGATAGGAT GATGAATAAG 
TTGTGGCCAC TAAAGCAATC ATTGTTAGTA AGAGAAGGTT CTGAGCCTGA CCACTTCTGG 
AAAACATTAG GAGGAAGATC AGAATACTCT AAAGAAAAGC TTGTCAAGGG TTGGCCTGCA 
GATCCACATA TGTATACTTG TACTTTCGAA CAATGTCTGT TCAAGGCAAA GGAGGTGTTC 
AGCTTCACGC AGGATGATCT TGCAACTGAG GAGACTTTGA TTGTGGACTG CAGCGAAGAA 
ATCTACGTCT GGGTCGGACT TCACTCCGGT GTCACGTCCA AGGAGCATGC TCTTGACATC 
GGCAAGATGT TCCTTCAAGC AGGCATTGGT CAGGATGGAC GGCGTTCAAT CGACACAACT 
GTATATGCAG TTGCAGAAGG GGAGGAACCT GCATTTTTCA CCACCTTTTT CGACTGGGAT 
AGTTCAAAGC AAGCAGAAGC TACGTTGGGG AACTCATTTG AGAGGAAGCT CGCGATGCTG 
AAAGGTCTTT CACCAAAATT GGAAGAGTCG TCGCCGGACA CCAGGATCAG CCTGCGCAAG 
TCACCGTCGA GAAGACCTGC AGCTGCAGGG AGACCATCGG AGCCAGCAGC CACGCCGGAG 
CAGCAACCGT CAGCAAGGAA GATTGCGTTC GGCTCTGCCG GGAGGTTGGC TAGAGAGAGA 
TCCCCATCAG CGTCCGGACT ACTGTCCCCA TCGTCAGCAT CATCGTCTCC TAAGAGCCGC 
GTGAACTCCT CGCCGTCGAC ACCGGCGGTG GCGCGGCGGC TCTTCAGCAG CTCCTCAATG 
CACGCGGCGG CCGAGGCAGC AGCAGCTGCC TCGCATGTCG CCTCCCCTAC GGCCAAAGGA 
TCACGACGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014753524.1
CDS	129..2483
Translation	MASDKAVELDAALGSQAVQYRETQGEESDEFLSYFRPCIIPIQGSFSSHWSRSGDECDRTTMFRCEGEHVPRVREVPFSRSSLDHSAAFIVDTPSKIFLFSGCNSSIQTRAKALDVIKHLRENRHCGRCEIGTIEDGKLVGDSDAGEFWNLFGGYAPIPRDVPGTVNGESMTTSPKKLFWINKRNLVPMEANLLDREMLNSDRSYILDCGTEIFLWIGITTLVSERKTSITALEDYVHCQGRSSIGRTVIVTEGHEIADFKLHFQHWPKNVVQKLYEAGREKVAAIFKHQGYDVAEIPEDKSPQLISSNGCLKVWLVDRGCATLLCTEEQEQLYNGDSYIIQYSYAEDGKDYHLFFAWSGQNSIQEDRVATVSLVSSMADSVKGHAVVGQMFEGREPELFFLVFKSLVIFKGGRSAAAAYMNSVLQNSNRTEWYQKDGTALFRVQGLRHDCIQAIQVNLATNSLNSSHCYILQDGASLFTWLGSLSSPGDHALLDRMMNKLWPLKQSLLVREGSEPDHFWKTLGGRSEYSKEKLVKGWPADPHMYTCTFEQCLFKAKEVFSFTQDDLATEETLIVDCSEEIYVWVGLHSGVTSKEHALDIGKMFLQAGIGQDGRRSIDTTVYAVAEGEEPAFFTTFFDWDSSKQAEATLGNSFERKLAMLKGLSPKLEESSPDTRISLRKSPSRRPAAAGRPSEPAATPEQQPSARKIAFGSAGRLARERSPSASGLLSPSSASSSPKSRVNSSPSTPAVARRLFSSSSMHAAAEAAAAASHVASPTAKGSRRR

Target ORF information:

RefSeq Version	XM_014898038.1
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon villin-1-like (LOC100826533), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014898038.1

ORF Insert Sequence:

ATGGCCTCGG ACAAGGCTGT CGAGTTGGAT GCGGCTCTGG GCTCCCAAGC GGTCCAGTAC 
AGGGAAACGC AAGGCGAAGA ATCCGACGAG TTCTTGTCAT ACTTTAGACC ATGCATTATA 
CCAATACAAG GTAGCTTCTC TTCGCACTGG AGTAGATCTG GGGACGAATG TGACAGGACT 
ACGATGTTTC GGTGTGAAGG AGAGCATGTA CCTCGAGTCA GAGAGGTTCC ATTTTCGCGT 
TCTTCACTGG ATCATAGTGC AGCATTTATA GTGGACACGC CGTCGAAGAT CTTTCTTTTC 
AGTGGTTGTA ATTCTAGTAT ACAGACAAGG GCTAAGGCAT TAGATGTCAT TAAGCACCTG 
AGAGAAAACC GACATTGCGG CAGATGTGAG ATTGGGACAA TAGAGGATGG AAAGCTTGTT 
GGTGATTCTG ATGCTGGTGA GTTCTGGAAC CTATTTGGAG GCTATGCACC TATTCCCCGT 
GATGTACCAG GCACGGTCAA TGGGGAGTCG ATGACTACCT CACCCAAGAA ACTTTTTTGG 
ATCAACAAAA GAAACCTTGT TCCCATGGAG GCAAATTTGT TAGACAGAGA AATGCTGAAC 
TCTGACAGGA GTTACATACT GGACTGTGGC ACTGAAATAT TCTTGTGGAT AGGGATAACA 
ACACTGGTTT CAGAGAGGAA GACATCAATT ACCGCCTTAG AGGATTATGT ACATTGTCAA 
GGCAGATCAT CAATTGGTCG TACTGTTATA GTGACTGAAG GTCATGAAAT TGCCGACTTC 
AAACTGCATT TTCAGCATTG GCCCAAGAAC GTTGTGCAAA AGTTATATGA GGCAGGGCGT 
GAGAAAGTGG CAGCAATTTT CAAACATCAG GGCTACGATG TTGCAGAAAT TCCGGAAGAT 
AAATCGCCGC AGCTCATCAG TAGTAATGGT TGTCTAAAGG TTTGGCTGGT AGACCGCGGT 
TGTGCAACCC TCCTTTGCAC TGAGGAGCAG GAGCAACTGT ACAATGGGGA CAGCTATATC 
ATACAGTATA GCTATGCTGA AGATGGAAAA GATTATCATC TGTTCTTTGC TTGGTCTGGA 
CAGAATAGCA TACAGGAAGA CAGAGTTGCT ACAGTTTCAT TGGTGTCAAG CATGGCCGAT 
TCAGTCAAAG GGCATGCAGT TGTCGGGCAA ATGTTTGAGG GCAGAGAACC AGAGTTGTTT 
TTCCTAGTAT TCAAGTCATT GGTTATATTC AAGGGGGGCA GGAGTGCTGC TGCTGCATAT 
ATGAACTCTG TTCTACAGAA TAGCAATAGA ACTGAATGGT ATCAGAAAGA TGGAACTGCA 
TTATTTAGGG TTCAAGGGCT GAGACATGAT TGCATACAAG CCATTCAGGT CAATCTGGCC 
ACAAATTCTC TCAATTCCTC GCACTGTTAC ATTTTACAAG ACGGTGCTTC TTTGTTTACC 
TGGCTAGGAA GCCTTTCTTC ACCAGGTGAT CATGCTCTAC TTGATAGGAT GATGAATAAG 
TTGTGGCCAC TAAAGCAATC ATTGTTAGTA AGAGAAGGTT CTGAGCCTGA CCACTTCTGG 
AAAACATTAG GAGGAAGATC AGAATACTCT AAAGAAAAGC TTGTCAAGGG TTGGCCTGCA 
GATCCACATA TGTATACTTG TACTTTCGAA CAATGTCTGT TCAAGGCAAA GGAGGTGTTC 
AGCTTCACGC AGGATGATCT TGCAACTGAG GAGACTTTGA TTGTGGACTG CAGCGAAGAA 
ATCTACGTCT GGGTCGGACT TCACTCCGGT GTCACGTCCA AGGAGCATGC TCTTGACATC 
GGCAAGATGT TCCTTCAAGC AGGCATTGGT CAGGATGGAC GGCGTTCAAT CGACACAACT 
GTATATGCAG TTGCAGAAGG GGAGGAACCT GCATTTTTCA CCACCTTTTT CGACTGGGAT 
AGTTCAAAGC AAGCAGAAGC TACGTTGGGG AACTCATTTG AGAGGAAGCT CGCGATGCTG 
AAAGGTCTTT CACCAAAATT GGAAGAGTCG TCGCCGGACA CCAGGATCAG CCTGCGCAAG 
TCACCGTCGA GAAGACCTGC AGCTGCAGGG AGACCATCGG AGCCAGCAGC CACGCCGGAG 
CAGCAACCGT CAGCAAGGAA GATTGCGTTC GGCTCTGCCG GGAGGTTGGC TAGAGAGAGA 
TCCCCATCAG CGTCCGGACT ACTGTCCCCA TCGTCAGCAT CATCGTCTCC TAAGAGCCGC 
GTGAACTCCT CGCCGTCGAC ACCGGCGGTG GCGCGGCGGC TCTTCAGCAG CTCCTCAATG 
CACGCGGCGG CCGAGGCAGC AGCAGCTGCC TCGCATGTCG CCTCCCCTAC GGCCAAAGGA 
TCACGACGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC100826533 cDNA ORF clone, Brachypodium distachyon(stiff brome)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム