MTCL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTCL1
Entrez Gene ID	538547
Full Name	microtubule crosslinking factor 1
Synonyms	CCDC165
General protein information	Preferred Names microtubule crosslinking factor 1 Names microtubule cross-linking factor 1 coiled-coil domain containing 165
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005224298.3	XP_005224355.1	microtubule cross-linking factor 1 isoform X1
XM_015469149.1	XP_015324635.1	microtubule cross-linking factor 1 isoform X2
XM_005224298.4	XP_005224355.2	microtubule cross-linking factor 1 isoform X1
XM_010818953.2	XP_010817255.1	microtubule cross-linking factor 1 isoform X2
XM_010825558.2	XP_010823860.2	microtubule cross-linking factor 1 isoform X1
XM_010818953.3	XP_010817255.2	microtubule cross-linking factor 1 isoform X2
XM_024984415.1	XP_024840183.1	microtubule cross-linking factor 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTCL1	NP_056025.2
Pan troglodytes (chimpanzee)	SOGA2	XP_001140422.2
Canis lupus familiaris (dog)	SOGA2	XP_005623132.1
Bos taurus (cattle)	CCDC165	XP_005224355.1
Danio rerio (zebrafish)	LOC101884972	XP_005171514.1
	LOC706392	XP_001100204.2
Mus musculus (house mouse)	Soga2	NP_766551.3
Rattus norvegicus (Norway rat)	Soga2	XP_006245692.1
Gallus gallus (chicken)	SOGA2	XP_004939798.1

Gene Ontology

The following MTCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa230006	XM_010818953.3 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa230005	XM_005224298.4 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa230007	XM_024984415.1 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa32643	XM_010825558.2 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32643	XM_005224298.3 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32645	XM_015469149.1 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32645	XM_010818953.2 Latest version!	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTCL1 ( XM_010818953.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817255.2 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230006
Clone ID Related Accession (Same CDS sequence)	XM_010818953.3
Accession Version	XM_010818953.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
GATGACAGCG CGGATCTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAGGG CTCGCTGATG 
CTCAGGAAGA TGGCCAAGCT GGGGCGCGAG AAGGACCGGC TGGAGCAGGA GCTGCAGAGG 
TACAGGGCCC TCTACGGGGA CGCTGACAGC CCCCTGCCCA CGGGCGAGGC CGGCGGGCCA 
CCCAGCACCC GGGAGGCGGA GCTGAAGCTG CGGCTGAGGC TGGTGGAGGA GGAGGCCAGC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAATC GGGGCCTCAG GGCTGAGATG 
GAAGACCTGC GCGGCCGGGA ATCCCTGCCC GGTGGGCCGA CCTCGCCCTT CGGGGGTGAC 
TCCCTGGAGT CCTCGGCTGA GCTGCGCCGG CACCTGCAGT TCGTGGAGGA GGAGGCGGAG 
CTGCTGCGGC GGTCCATCTC CGAGATCGAG GACCACAACC GGCAGCTGAC CCACGAGCTG 
AGCAAGTTCA AGTTCGAGCC GCGGCCCGAG CCGGGCTGGC TGGCAGGGGG CGGGGCGCCC 
CTGCAGGACG AGCTCCAGGC GGTGCGGCTG CCGATAAGCG AGCTGAGCGG GGAGGTGCTG 
ACGCTGCAGC CGGGCCCGCA CGCCCCGAGC CCGCGGGACA GTGATGCTGA CAGCGACACG 
GGCCGAAGGA GCGACGACGG TGAGGACGGA CGCGTGCAGC CTCGCCGGGA GGGCCCCATT 
GGCGGCGAGA GTGACTCGGA GGAGACCTTC GAGAAGACGT CTGGCTTCGG AAGCGGGAGG 
CCGTCGGAGG CCGGCGAGTG CCCGGCGGAG CTGCTGCGCG CGCGGGAGGA CTCGGAGCGC 
CTGGCGAGCA TCAGGCACGA GGCGGAGCGG CTGGAGCGCA CGGTGGAGCG CATCATCACG 
GACACCGAGG CCGCTCTCCC GGGCGGAGAG GCCTCGCCCG GGGCCTGCGG CCAGGGCATC 
AGGGAGGAGC CCCAGCTGCT GGGGACCCTC AACGCCAGGA TGAAGGCATT CAGGAAAGAG 
CTCCAGGCCT TCCTGGAGCA GGTGGAGCGA CTGGGGGAGG GCCTGTCCCC GCTGCCCCAG 
CTCACCGAGT CATCCAGCTT CCTGTCCTCT GTGACCTCCG TATCCCGGGA CTCCCCGGTG 
GGGAACCTGG GCAAGGAGCT GGGCCCGGAC CTGCAGTCCA AACTGAGAGA TCAGATGGAG 
TGGCAGCCGG GGCAGGAGCG AGGGGAGGAG CGGGGTCACC TCCATCTCCG CACCGCACGG 
GAGCTGCACC GCCGAGCCGA CGGGGATGCC GGAAGCTGCC GGCCTGGAGC CCAGAGCTGC 
TTCAGCCTCG AGGCGGAGGA GGAACGCCGC TGCGCCCTGC GGAGGCGCGA CCGGGAGGCA 
AACGGCCTGG CCCTGCAGAG CTCGCTGCAC GAGCGCGCCT GGGACGACGA GAGGCACCTG 
ATGCAGCAGG AGCTGCGCTC CCTGAAGCAG AACATCTTCC TCTTCTACGT CAAGCTCAGG 
TGGCTGCTCA AGCACTGGCG CCAAGGGAGG CAGGCGGAGG ACGACGCGGA GGACTTCATG 
GAGAGCGAGC TTCCAGAGAC CTTCCCCAGG CTCGGGGAGC TTGGCGTCCA GGGGGACACA 
AAGGCGGACA GTCCAGACCG CGACGACGAT GGGCCAAGCT GTGGGTTTTC CCTGGGAGAG 
CGCTCTCCGC AGATGGGTGA CCACGGGTCA CGGCTGCAGC CCACCGACGG GGGACAGCTC 
TACAGGCAGG TGGCAGAGAC CCAGCCGCTG TTCTGCGCCC TCAAGGCACT GCTTGAGGAG 
CTGCGCGCAG AGCTACGGGA GGACGCGCGC GCACGCCAGC GGCTGCAGCA GCAATACGCA 
GGTGACAAGG CTGCCTGGGA TGTGGAGTGG GCCGCGCTCA CATGCCGCCT GGAGCAGCTG 
GAAGCAAAGA ATGAAAAGAG CTTGGGAGAC CCAGGCTCCT GCACCGACAG CAAAGGGGCA 
TTCAGGAAGG AGAGGGAGGT GCAGCAGAAG CTGCTGGCGG ACAGTCACAG GCTGGTCATG 
GACCTGCGCT GGCAGATCCA CCACAGCGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTT 
CTCGACCGCC TGGACCGGGA GCGCCAGGAG TGGGGGCGGC AGGAGAAGGA GATGCTGTGG 
AGAATTGAAC AGTTGCAGAA AGAAAACAGT CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT 
CAAGAGGAGG GGGACACCCG CCCCTGTACC CACCCAGGGA GCCCTCGGGC GCCCCGCCCT 
GCGGGGATGT GGCCCTGTAT GGACACAGAC TCCAGTGCGT TTGAGGACCG GCCGCTGTCA 
AAGCTGAAGG AATCGGACAG GTGCTCAGCG CGGGAGAACC TCTACCTGGA CACCTTGTCC 
CTGGACGACG AGCCAGAGGA GCCCCCGGCC CGTGGGCCCC AGAGGGAGTT CAGGAACTGC 
TTCCGTGAGG AAGAAGAAAA TCACAAGGGA AATCTTCAAA GGGCCGTGTC TGTGTCGTCC 
ATGTCTGAGT TCCAGCGCTT GATGGATGGC TCTCCCTTCC TGCCGGAGAA GGGCCTGCCC 
CCCACATGTA GCAAGGAGGA CGTCACCCCA CCCCTGTCCC CTGATGACCT CAAGTACATC 
GAGGAGTTCA ACAGCAAGAG CTGGGACGAC AGGCCTCCAG ACCCCTGGGC GGACAGGACC 
GAGGTGGGGC GGACGCGGCC CGAGGCCAGC ACTGAACCTT TCTCTGACGC CGCCTGGTAC 
CTCACCACAA GCATCACCCT GACCACGGAC ACCGTGACCA ACCCCGAACA CTGCCAGAAG 
CAGCCCCCGC GGAACCTTGT CTGTGCTGAG CAGGCTGGGG GGAGGGGGCT GCACAGCCCA 
CCCGCCATCC GGAGGCTGGA GGGCATCATG GCGGGGGTCG GCGAGAGCAG GGTCCGAGGG 
GGCCCCGGGG ATGCCAAGGG GAGCCCTTCT GAACATGTGC TCAGCAGGTG GCCCTGTGCC 
CCCTCCAGGC ACCCCCGAGA CTACACGGAG GTGGGGCTCC GGCCCCTCGA TAGCACCCTC 
AGCTTCTCCT CCCCATTGCA CAGCCTGGAA ATGCCCAGAA ACCTGAGTGA TGATATGAAG 
GAGGTAGCCT TCTCGGTCAG GAGCGCCATC TGCTCCAGCC CCGTTGAGCC TCTGGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACAACAGGAA CACAGACAGT CCAGACCATC 
AGTGTTGGCC TACAGACGGA AGCCCTGCGG GGCAGCCCAC ACAAGTGTCT CACACCCAAG 
GCGGGAGGTG GTGCCACACC CGCGTCCTCT CCCTCCCGTG GCCTCAGGAA CAGGCAGGTG 
GCCCCCGCCA TTGAGAAGGT GCAAGCTAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAC 
GGGTCTCCCA AGCTGCAGCG GAAGCCCCTT CCCAGAGTGG ACCAGTCAAG TAGCAGGGCC 
TCCCCGGGGG TGGCCCAGAA GGGGTGTGGC GAGTCAGCCT GGGCCCGCTC CACCACCACA 
CGCGAGAGCC CCGTGCACAC CGCCATGAAC GATGGCCTCT CCAGCCTCTT CAGCATCATC 
GACCACAGCC CGGTGGTGCA GGACCCCTTC CAGAAGGGGG TGCGGACTGC GAGTCGGTCT 
CGCTCGGCCG AGCCCCGGCC AGAGCTGGGT CCAGGCCAGG ACACCAGCCC CAGCTCTCGG 
GGCCGCTCAC CCAGCCCCAT TGGGGGTGGC TCAGAGACAT GCAGGGAGGA GGGGGGAGAG 
GGCACGCCCC TGAGGCAGGA CTTATCTGCT CCCCCTGGCT ACACACTCAC CGAGAACGTG 
GCCCGGATCC TCCACAGAAA GCTGTTGGAA CATGCCTTAA AGGAGGAGCG GAGGCAGGCT 
GCCCACAGCC CCACAAATAG CAGCAGCGAC AACCACATGG GAGAAGCGGT GGTCAAGGCC 
GAACCAGGGT CCATCGAGAA CCAAACTGTC TTACTAACTG CCCCCTGGGG ACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817255.2
CDS	582..5078
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_010818953.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818953.3

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
GATGACAGCG CGGATCTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAGGG CTCGCTGATG 
CTCAGGAAGA TGGCCAAGCT GGGGCGCGAG AAGGACCGGC TGGAGCAGGA GCTGCAGAGG 
TACAGGGCCC TCTACGGGGA CGCTGACAGC CCCCTGCCCA CGGGCGAGGC CGGCGGGCCA 
CCCAGCACCC GGGAGGCGGA GCTGAAGCTG CGGCTGAGGC TGGTGGAGGA GGAGGCCAGC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAATC GGGGCCTCAG GGCTGAGATG 
GAAGACCTGC GCGGCCGGGA ATCCCTGCCC GGTGGGCCGA CCTCGCCCTT CGGGGGTGAC 
TCCCTGGAGT CCTCGGCTGA GCTGCGCCGG CACCTGCAGT TCGTGGAGGA GGAGGCGGAG 
CTGCTGCGGC GGTCCATCTC CGAGATCGAG GACCACAACC GGCAGCTGAC CCACGAGCTG 
AGCAAGTTCA AGTTCGAGCC GCGGCCCGAG CCGGGCTGGC TGGCAGGGGG CGGGGCGCCC 
CTGCAGGACG AGCTCCAGGC GGTGCGGCTG CCGATAAGCG AGCTGAGCGG GGAGGTGCTG 
ACGCTGCAGC CGGGCCCGCA CGCCCCGAGC CCGCGGGACA GTGATGCTGA CAGCGACACG 
GGCCGAAGGA GCGACGACGG TGAGGACGGA CGCGTGCAGC CTCGCCGGGA GGGCCCCATT 
GGCGGCGAGA GTGACTCGGA GGAGACCTTC GAGAAGACGT CTGGCTTCGG AAGCGGGAGG 
CCGTCGGAGG CCGGCGAGTG CCCGGCGGAG CTGCTGCGCG CGCGGGAGGA CTCGGAGCGC 
CTGGCGAGCA TCAGGCACGA GGCGGAGCGG CTGGAGCGCA CGGTGGAGCG CATCATCACG 
GACACCGAGG CCGCTCTCCC GGGCGGAGAG GCCTCGCCCG GGGCCTGCGG CCAGGGCATC 
AGGGAGGAGC CCCAGCTGCT GGGGACCCTC AACGCCAGGA TGAAGGCATT CAGGAAAGAG 
CTCCAGGCCT TCCTGGAGCA GGTGGAGCGA CTGGGGGAGG GCCTGTCCCC GCTGCCCCAG 
CTCACCGAGT CATCCAGCTT CCTGTCCTCT GTGACCTCCG TATCCCGGGA CTCCCCGGTG 
GGGAACCTGG GCAAGGAGCT GGGCCCGGAC CTGCAGTCCA AACTGAGAGA TCAGATGGAG 
TGGCAGCCGG GGCAGGAGCG AGGGGAGGAG CGGGGTCACC TCCATCTCCG CACCGCACGG 
GAGCTGCACC GCCGAGCCGA CGGGGATGCC GGAAGCTGCC GGCCTGGAGC CCAGAGCTGC 
TTCAGCCTCG AGGCGGAGGA GGAACGCCGC TGCGCCCTGC GGAGGCGCGA CCGGGAGGCA 
AACGGCCTGG CCCTGCAGAG CTCGCTGCAC GAGCGCGCCT GGGACGACGA GAGGCACCTG 
ATGCAGCAGG AGCTGCGCTC CCTGAAGCAG AACATCTTCC TCTTCTACGT CAAGCTCAGG 
TGGCTGCTCA AGCACTGGCG CCAAGGGAGG CAGGCGGAGG ACGACGCGGA GGACTTCATG 
GAGAGCGAGC TTCCAGAGAC CTTCCCCAGG CTCGGGGAGC TTGGCGTCCA GGGGGACACA 
AAGGCGGACA GTCCAGACCG CGACGACGAT GGGCCAAGCT GTGGGTTTTC CCTGGGAGAG 
CGCTCTCCGC AGATGGGTGA CCACGGGTCA CGGCTGCAGC CCACCGACGG GGGACAGCTC 
TACAGGCAGG TGGCAGAGAC CCAGCCGCTG TTCTGCGCCC TCAAGGCACT GCTTGAGGAG 
CTGCGCGCAG AGCTACGGGA GGACGCGCGC GCACGCCAGC GGCTGCAGCA GCAATACGCA 
GGTGACAAGG CTGCCTGGGA TGTGGAGTGG GCCGCGCTCA CATGCCGCCT GGAGCAGCTG 
GAAGCAAAGA ATGAAAAGAG CTTGGGAGAC CCAGGCTCCT GCACCGACAG CAAAGGGGCA 
TTCAGGAAGG AGAGGGAGGT GCAGCAGAAG CTGCTGGCGG ACAGTCACAG GCTGGTCATG 
GACCTGCGCT GGCAGATCCA CCACAGCGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTT 
CTCGACCGCC TGGACCGGGA GCGCCAGGAG TGGGGGCGGC AGGAGAAGGA GATGCTGTGG 
AGAATTGAAC AGTTGCAGAA AGAAAACAGT CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT 
CAAGAGGAGG GGGACACCCG CCCCTGTACC CACCCAGGGA GCCCTCGGGC GCCCCGCCCT 
GCGGGGATGT GGCCCTGTAT GGACACAGAC TCCAGTGCGT TTGAGGACCG GCCGCTGTCA 
AAGCTGAAGG AATCGGACAG GTGCTCAGCG CGGGAGAACC TCTACCTGGA CACCTTGTCC 
CTGGACGACG AGCCAGAGGA GCCCCCGGCC CGTGGGCCCC AGAGGGAGTT CAGGAACTGC 
TTCCGTGAGG AAGAAGAAAA TCACAAGGGA AATCTTCAAA GGGCCGTGTC TGTGTCGTCC 
ATGTCTGAGT TCCAGCGCTT GATGGATGGC TCTCCCTTCC TGCCGGAGAA GGGCCTGCCC 
CCCACATGTA GCAAGGAGGA CGTCACCCCA CCCCTGTCCC CTGATGACCT CAAGTACATC 
GAGGAGTTCA ACAGCAAGAG CTGGGACGAC AGGCCTCCAG ACCCCTGGGC GGACAGGACC 
GAGGTGGGGC GGACGCGGCC CGAGGCCAGC ACTGAACCTT TCTCTGACGC CGCCTGGTAC 
CTCACCACAA GCATCACCCT GACCACGGAC ACCGTGACCA ACCCCGAACA CTGCCAGAAG 
CAGCCCCCGC GGAACCTTGT CTGTGCTGAG CAGGCTGGGG GGAGGGGGCT GCACAGCCCA 
CCCGCCATCC GGAGGCTGGA GGGCATCATG GCGGGGGTCG GCGAGAGCAG GGTCCGAGGG 
GGCCCCGGGG ATGCCAAGGG GAGCCCTTCT GAACATGTGC TCAGCAGGTG GCCCTGTGCC 
CCCTCCAGGC ACCCCCGAGA CTACACGGAG GTGGGGCTCC GGCCCCTCGA TAGCACCCTC 
AGCTTCTCCT CCCCATTGCA CAGCCTGGAA ATGCCCAGAA ACCTGAGTGA TGATATGAAG 
GAGGTAGCCT TCTCGGTCAG GAGCGCCATC TGCTCCAGCC CCGTTGAGCC TCTGGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACAACAGGAA CACAGACAGT CCAGACCATC 
AGTGTTGGCC TACAGACGGA AGCCCTGCGG GGCAGCCCAC ACAAGTGTCT CACACCCAAG 
GCGGGAGGTG GTGCCACACC CGCGTCCTCT CCCTCCCGTG GCCTCAGGAA CAGGCAGGTG 
GCCCCCGCCA TTGAGAAGGT GCAAGCTAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAC 
GGGTCTCCCA AGCTGCAGCG GAAGCCCCTT CCCAGAGTGG ACCAGTCAAG TAGCAGGGCC 
TCCCCGGGGG TGGCCCAGAA GGGGTGTGGC GAGTCAGCCT GGGCCCGCTC CACCACCACA 
CGCGAGAGCC CCGTGCACAC CGCCATGAAC GATGGCCTCT CCAGCCTCTT CAGCATCATC 
GACCACAGCC CGGTGGTGCA GGACCCCTTC CAGAAGGGGG TGCGGACTGC GAGTCGGTCT 
CGCTCGGCCG AGCCCCGGCC AGAGCTGGGT CCAGGCCAGG ACACCAGCCC CAGCTCTCGG 
GGCCGCTCAC CCAGCCCCAT TGGGGGTGGC TCAGAGACAT GCAGGGAGGA GGGGGGAGAG 
GGCACGCCCC TGAGGCAGGA CTTATCTGCT CCCCCTGGCT ACACACTCAC CGAGAACGTG 
GCCCGGATCC TCCACAGAAA GCTGTTGGAA CATGCCTTAA AGGAGGAGCG GAGGCAGGCT 
GCCCACAGCC CCACAAATAG CAGCAGCGAC AACCACATGG GAGAAGCGGT GGTCAAGGCC 
GAACCAGGGT CCATCGAGAA CCAAACTGTC TTACTAACTG CCCCCTGGGG ACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005224298.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224355.2 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa230005
Clone ID Related Accession (Same CDS sequence)	XM_005224298.4
Accession Version	XM_005224298.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224298.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
GATGACAGCG CGGATCTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAGGG CTCGCTGATG 
CTCAGGAAGA TGGCCAAGCT GGGGCGCGAG AAGGACCGGC TGGAGCAGGA GCTGCAGAGG 
TACAGGGCCC TCTACGGGGA CGCTGACAGC CCCCTGCCCA CGGGCGAGGC CGGCGGGCCA 
CCCAGCACCC GGGAGGCGGA GCTGAAGCTG CGGCTGAGGC TGGTGGAGGA GGAGGCCAGC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAATC GGGGCCTCAG GGCTGAGATG 
GAAGACCTGC GCGGCCGGGA ATCCCTGCCC GGTGGGCCGA CCTCGCCCTT CGGGGGTGAC 
TCCCTGGAGT CCTCGGCTGA GCTGCGCCGG CACCTGCAGT TCGTGGAGGA GGAGGCGGAG 
CTGCTGCGGC GGTCCATCTC CGAGATCGAG GACCACAACC GGCAGCTGAC CCACGAGCTG 
AGCAAGTTCA AGTTCGAGCC GCGGCCCGAG CCGGGCTGGC TGGCAGGGGG CGGGGCGCCC 
CTGCAGGACG AGCTCCAGGC GGTGCGGCTG CCGATAAGCG AGCTGAGCGG GGAGGTGCTG 
ACGCTGCAGC CGGGCCCGCA CGCCCCGAGC CCGCGGGACA GTGATGCTGA CAGCGACACG 
GGCCGAAGGA GCGACGACGG TGAGGACGGA CGCGTGCAGC CTCGCCGGGA GGGCCCCATT 
GGCGGCGAGA GTGACTCGGA GGAGACCTTC GAGAAGACGT CTGGCTTCGG AAGCGGGAGG 
CCGTCGGAGG CCGGCGAGTG CCCGGCGGAG CTGCTGCGCG CGCGGGAGGA CTCGGAGCGC 
CTGGCGAGCA TCAGGCACGA GGCGGAGCGG CTGGAGCGCA CGGTGGAGCG CATCATCACG 
GACACCGAGG CCGCTCTCCC GGGCGGAGAG GCCTCGCCCG GGGCCTGCGG CCAGGGCATC 
AGGGAGGAGC CCCAGCTGCT GGGGACCCTC AACGCCAGGA TGAAGGCATT CAGGAAAGAG 
CTCCAGGCCT TCCTGGAGCA GGTGGAGCGA CTGGGGGAGG GCCTGTCCCC GCTGCCCCAG 
CTCACCGAGT CATCCAGCTT CCTGTCCTCT GTGACCTCCG TATCCCGGGA CTCCCCGGTG 
GGGAACCTGG GCAAGGAGCT GGGCCCGGAC CTGCAGTCCA AACTGAGAGA TCAGATGGAG 
TGGCAGCCGG GGCAGGAGCG AGGGGAGGAG CGGGGTCACC TCCATCTCCG CACCGCACGG 
GAGCTGCACC GCCGAGCCGA CGGGGATGCC GGAAGCTGCC GGCCTGGAGC CCAGAGCTGC 
TTCAGCCTCG AGGCGGAGGA GGAACGCCGC TGCGCCCTGC GGAGGCGCGA CCGGGAGGCA 
AACGGCCTGG CCCTGCAGAG CTCGCTGCAC GAGCGCGCCT GGGACGACGA GAGGCACCTG 
ATGCAGCAGG AGCTGCGCTC CCTGAAGCAG AACATCTTCC TCTTCTACGT CAAGCTCAGG 
TGGCTGCTCA AGCACTGGCG CCAAGGGAGG CAGGCGGAGG ACGACGCGGA GGACTTCATG 
GAGAGCGAGC TTCCAGAGAC CTTCCCCAGG CTCGGGGAGC TTGGCGTCCA GGGGGACACA 
AAGGCGGACA GTCCAGACCG CGACGACGAT GGGCCAAGCT GTGGGTTTTC CCTGGGAGAG 
CGCTCTCCGC AGATGGGTGA CCACGGGTCA CGGCTGCAGC CCACCGACGG GGGACAGCTC 
TACAGGCAGG TGGCAGAGAC CCAGCCGCTG TTCTGCGCCC TCAAGGCACT GCTTGAGGAG 
CTGCGCGCAG AGCTACGGGA GGACGCGCGC GCACGCCAGC GGCTGCAGCA GCAATACGCA 
GGTGACAAGG CTGCCTGGGA TGTGGAGTGG GCCGCGCTCA CATGCCGCCT GGAGCAGCTG 
GAAGCAAAGA ATGAAAAGAG CTTGGGAGAC CCAGGCTCCT GCACCGACAG CAAAGGGGCA 
TTCAGGAAGG AGAGGGAGGT GCAGCAGAAG CTGCTGGCGG ACAGTCACAG GCTGGTCATG 
GACCTGCGCT GGCAGATCCA CCACAGCGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTT 
CTCGACCGCC TGGACCGGGA GCGCCAGGAG TGGGGGCGGC AGGAGAAGGA GATGCTGTGG 
AGAATTGAAC AGTTGCAGAA AGAAAACAGT CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT 
CAAGAGGAGG GGGACACCCG CCCCTGTACC CACCCAGGGA GCCCTCGGGC GCCCCGCCCT 
GCGGGGATGT GGCCCTGTAT GGACACAGAC TCCAGTGCGT TTGAGGACCG GCCGCTGTCA 
AAGCTGAAGG AATCGGACAG GTGCTCAGCG CGGGAGAACC TCTACCTGGA CACCTTGTCC 
CTGGACGACG AGCCAGAGGA GCCCCCGGCC CGTGGGCCCC AGAGGGAGTT CAGGAACTGC 
TTCCGTGAGG AAGAAGAAAA TCACAAGGGA AATCTTCAAA GGGCCGTGTC TGTGTCGTCC 
ATGTCTGAGT TCCAGCGCTT GATGGATGGC TCTCCCTTCC TGCCGGAGAA GGGCCTGCCC 
CCCACATGTA GCAAGGAGGA CGTCACCCCA CCCCTGTCCC CTGATGACCT CAAGTACATC 
GAGGAGTTCA ACAGCAAGAG CTGGGACGAC AGGCCTCCAG ACCCCTGGGC GGACAGGACC 
GAGGTGGGGC GGACGCGGCC CGAGGCCAGC ACTGAACCTT TCTCTGACGC CGCCTGGTAC 
CTCACCACAA GCATCACCCT GACCACGGAC ACCGTGACCA ACCCCGAACA CTGCCAGAAG 
CAGCCCCCGC GGAACCTTGT CTGTGCTGAG CAGGCTGGGG GGAGGGGGCT GCACAGCCCA 
CCCGCCATCC GGAGGCTGGA GGGCATCATG GCGGGGGTCG GCGAGAGCAG GGTCCGAGGG 
GGCCCCGGGG ATGCCAAGGG GAGCCCTTCT GAACATGTGC TCAGCAGGTG GCCCTGTGCC 
CCCTCCAGGC ACCCCCGAGA CTACACGGAG GTGGGGCTCC GGCCCCTCGA TAGCACCCTC 
AGCTTCTCCT CCCCATTGCA CAGCCTGGAA ATGCCCAGAA ACCTGAGTGA TGATATGAAG 
GAGGTAGCCT TCTCGGTCAG GAGCGCCATC TGCTCCAGCC CCGTTGAGCC TCTGGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACAACAGGAA CACAGACAGT CCAGACCATC 
AGTGTTGGCC TACAGACGGA AGCCCTGCGG GGCAGCCCAC ACAAGTGTCT CACACCCAAG 
GCGGGAGGTG GTGCCACACC CGCGTCCTCT CCCTCCCGTG GCCTCAGGAA CAGGCAGGTG 
GCCCCCGCCA TTGAGAAGGT GCAAGCTAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAC 
GGGTCTCCCA AGCTGCAGCG GAAGCCCCTT CCCAGAGTGG ACCAGTCAAG TAGCAGGGCC 
TCCCCGGGGG TGGCCCAGAA GGGGTGTGGC GAGTCAGCCT GGGCCCGCTC CACCACCACA 
CGCGAGAGCC CCGTGCACAC CGCCATGAAC GATGGCCTCT CCAGCCTCTT CAGCATCATC 
GACCACAGCC CGGTGGTGCA GGACCCCTTC CAGAAGGGGG TGCGGACTGC GAGTCGGTCT 
CGCTCGGCCG AGCCCCGGCC AGAGCTGGGT CCAGGCCAGG ACACCAGCCC CAGCTCTCGG 
GGCCGCTCAC CCAGCCCCAT TGGGGGTGGC TCAGAGACAT GCAGGGAGGA GGGGGGAGAG 
GGCACGCCCC TGAGGCAGGA CTTATCTGCT CCCCCTGGCT ACACACTCAC CGAGAACGTG 
GCCCGGATCC TCCACAGAAA GCTGTTGGAA CATGCCTTAA AGGAGGAGCG GAGGCAGGCT 
GCCCACAGCC CCACAAATAG CAGCAGCGAC AACCACATGG GAGAAGCGGT GGTCAAGGCC 
GAACCAGGGT CCATCGAGGA ACTACCTTGT TCTGCACTTG CTCCATCCCT AGAGCCTTGC 
TTCTCCAGGC CCGAGAGACC AGCAAACCGT CGCCCTCCGT CCCGCTGGGC CCCACACCCC 
GCCACTGCCT CACCAGCTCC ATCACCCGGA GAACCCACCT CCTTGGAGGA GCTCGGTGAT 
GAGGAGCCAC CTGGGGAGAA GCCGCACGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224355.2
CDS	578..5230
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHPATASPAPSPGEPTSLEELGDEEPPGEKPHV

Target ORF information:

RefSeq Version	XM_005224298.4
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224298.4

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
GATGACAGCG CGGATCTGAG GTGCCAGCTC CAGTTTGCCA AAGAGGAGGG CTCGCTGATG 
CTCAGGAAGA TGGCCAAGCT GGGGCGCGAG AAGGACCGGC TGGAGCAGGA GCTGCAGAGG 
TACAGGGCCC TCTACGGGGA CGCTGACAGC CCCCTGCCCA CGGGCGAGGC CGGCGGGCCA 
CCCAGCACCC GGGAGGCGGA GCTGAAGCTG CGGCTGAGGC TGGTGGAGGA GGAGGCCAGC 
ATCTTGGGCC GGAAGATCGT GGAGCTGGAG GTGGAGAATC GGGGCCTCAG GGCTGAGATG 
GAAGACCTGC GCGGCCGGGA ATCCCTGCCC GGTGGGCCGA CCTCGCCCTT CGGGGGTGAC 
TCCCTGGAGT CCTCGGCTGA GCTGCGCCGG CACCTGCAGT TCGTGGAGGA GGAGGCGGAG 
CTGCTGCGGC GGTCCATCTC CGAGATCGAG GACCACAACC GGCAGCTGAC CCACGAGCTG 
AGCAAGTTCA AGTTCGAGCC GCGGCCCGAG CCGGGCTGGC TGGCAGGGGG CGGGGCGCCC 
CTGCAGGACG AGCTCCAGGC GGTGCGGCTG CCGATAAGCG AGCTGAGCGG GGAGGTGCTG 
ACGCTGCAGC CGGGCCCGCA CGCCCCGAGC CCGCGGGACA GTGATGCTGA CAGCGACACG 
GGCCGAAGGA GCGACGACGG TGAGGACGGA CGCGTGCAGC CTCGCCGGGA GGGCCCCATT 
GGCGGCGAGA GTGACTCGGA GGAGACCTTC GAGAAGACGT CTGGCTTCGG AAGCGGGAGG 
CCGTCGGAGG CCGGCGAGTG CCCGGCGGAG CTGCTGCGCG CGCGGGAGGA CTCGGAGCGC 
CTGGCGAGCA TCAGGCACGA GGCGGAGCGG CTGGAGCGCA CGGTGGAGCG CATCATCACG 
GACACCGAGG CCGCTCTCCC GGGCGGAGAG GCCTCGCCCG GGGCCTGCGG CCAGGGCATC 
AGGGAGGAGC CCCAGCTGCT GGGGACCCTC AACGCCAGGA TGAAGGCATT CAGGAAAGAG 
CTCCAGGCCT TCCTGGAGCA GGTGGAGCGA CTGGGGGAGG GCCTGTCCCC GCTGCCCCAG 
CTCACCGAGT CATCCAGCTT CCTGTCCTCT GTGACCTCCG TATCCCGGGA CTCCCCGGTG 
GGGAACCTGG GCAAGGAGCT GGGCCCGGAC CTGCAGTCCA AACTGAGAGA TCAGATGGAG 
TGGCAGCCGG GGCAGGAGCG AGGGGAGGAG CGGGGTCACC TCCATCTCCG CACCGCACGG 
GAGCTGCACC GCCGAGCCGA CGGGGATGCC GGAAGCTGCC GGCCTGGAGC CCAGAGCTGC 
TTCAGCCTCG AGGCGGAGGA GGAACGCCGC TGCGCCCTGC GGAGGCGCGA CCGGGAGGCA 
AACGGCCTGG CCCTGCAGAG CTCGCTGCAC GAGCGCGCCT GGGACGACGA GAGGCACCTG 
ATGCAGCAGG AGCTGCGCTC CCTGAAGCAG AACATCTTCC TCTTCTACGT CAAGCTCAGG 
TGGCTGCTCA AGCACTGGCG CCAAGGGAGG CAGGCGGAGG ACGACGCGGA GGACTTCATG 
GAGAGCGAGC TTCCAGAGAC CTTCCCCAGG CTCGGGGAGC TTGGCGTCCA GGGGGACACA 
AAGGCGGACA GTCCAGACCG CGACGACGAT GGGCCAAGCT GTGGGTTTTC CCTGGGAGAG 
CGCTCTCCGC AGATGGGTGA CCACGGGTCA CGGCTGCAGC CCACCGACGG GGGACAGCTC 
TACAGGCAGG TGGCAGAGAC CCAGCCGCTG TTCTGCGCCC TCAAGGCACT GCTTGAGGAG 
CTGCGCGCAG AGCTACGGGA GGACGCGCGC GCACGCCAGC GGCTGCAGCA GCAATACGCA 
GGTGACAAGG CTGCCTGGGA TGTGGAGTGG GCCGCGCTCA CATGCCGCCT GGAGCAGCTG 
GAAGCAAAGA ATGAAAAGAG CTTGGGAGAC CCAGGCTCCT GCACCGACAG CAAAGGGGCA 
TTCAGGAAGG AGAGGGAGGT GCAGCAGAAG CTGCTGGCGG ACAGTCACAG GCTGGTCATG 
GACCTGCGCT GGCAGATCCA CCACAGCGAG AAGAACTGGA ACCGGGAGAA GGTGGAGCTT 
CTCGACCGCC TGGACCGGGA GCGCCAGGAG TGGGGGCGGC AGGAGAAGGA GATGCTGTGG 
AGAATTGAAC AGTTGCAGAA AGAAAACAGT CCCCGGAGAG GTGGCAGTTT CCTCTGTGAT 
CAAGAGGAGG GGGACACCCG CCCCTGTACC CACCCAGGGA GCCCTCGGGC GCCCCGCCCT 
GCGGGGATGT GGCCCTGTAT GGACACAGAC TCCAGTGCGT TTGAGGACCG GCCGCTGTCA 
AAGCTGAAGG AATCGGACAG GTGCTCAGCG CGGGAGAACC TCTACCTGGA CACCTTGTCC 
CTGGACGACG AGCCAGAGGA GCCCCCGGCC CGTGGGCCCC AGAGGGAGTT CAGGAACTGC 
TTCCGTGAGG AAGAAGAAAA TCACAAGGGA AATCTTCAAA GGGCCGTGTC TGTGTCGTCC 
ATGTCTGAGT TCCAGCGCTT GATGGATGGC TCTCCCTTCC TGCCGGAGAA GGGCCTGCCC 
CCCACATGTA GCAAGGAGGA CGTCACCCCA CCCCTGTCCC CTGATGACCT CAAGTACATC 
GAGGAGTTCA ACAGCAAGAG CTGGGACGAC AGGCCTCCAG ACCCCTGGGC GGACAGGACC 
GAGGTGGGGC GGACGCGGCC CGAGGCCAGC ACTGAACCTT TCTCTGACGC CGCCTGGTAC 
CTCACCACAA GCATCACCCT GACCACGGAC ACCGTGACCA ACCCCGAACA CTGCCAGAAG 
CAGCCCCCGC GGAACCTTGT CTGTGCTGAG CAGGCTGGGG GGAGGGGGCT GCACAGCCCA 
CCCGCCATCC GGAGGCTGGA GGGCATCATG GCGGGGGTCG GCGAGAGCAG GGTCCGAGGG 
GGCCCCGGGG ATGCCAAGGG GAGCCCTTCT GAACATGTGC TCAGCAGGTG GCCCTGTGCC 
CCCTCCAGGC ACCCCCGAGA CTACACGGAG GTGGGGCTCC GGCCCCTCGA TAGCACCCTC 
AGCTTCTCCT CCCCATTGCA CAGCCTGGAA ATGCCCAGAA ACCTGAGTGA TGATATGAAG 
GAGGTAGCCT TCTCGGTCAG GAGCGCCATC TGCTCCAGCC CCGTTGAGCC TCTGGTCAAG 
GACATGGCCT GCCAGACCAA TGGGTCCCGG ACAACAGGAA CACAGACAGT CCAGACCATC 
AGTGTTGGCC TACAGACGGA AGCCCTGCGG GGCAGCCCAC ACAAGTGTCT CACACCCAAG 
GCGGGAGGTG GTGCCACACC CGCGTCCTCT CCCTCCCGTG GCCTCAGGAA CAGGCAGGTG 
GCCCCCGCCA TTGAGAAGGT GCAAGCTAAG TTTGAACGCA CATGCTGCTC CCCCAAGTAC 
GGGTCTCCCA AGCTGCAGCG GAAGCCCCTT CCCAGAGTGG ACCAGTCAAG TAGCAGGGCC 
TCCCCGGGGG TGGCCCAGAA GGGGTGTGGC GAGTCAGCCT GGGCCCGCTC CACCACCACA 
CGCGAGAGCC CCGTGCACAC CGCCATGAAC GATGGCCTCT CCAGCCTCTT CAGCATCATC 
GACCACAGCC CGGTGGTGCA GGACCCCTTC CAGAAGGGGG TGCGGACTGC GAGTCGGTCT 
CGCTCGGCCG AGCCCCGGCC AGAGCTGGGT CCAGGCCAGG ACACCAGCCC CAGCTCTCGG 
GGCCGCTCAC CCAGCCCCAT TGGGGGTGGC TCAGAGACAT GCAGGGAGGA GGGGGGAGAG 
GGCACGCCCC TGAGGCAGGA CTTATCTGCT CCCCCTGGCT ACACACTCAC CGAGAACGTG 
GCCCGGATCC TCCACAGAAA GCTGTTGGAA CATGCCTTAA AGGAGGAGCG GAGGCAGGCT 
GCCCACAGCC CCACAAATAG CAGCAGCGAC AACCACATGG GAGAAGCGGT GGTCAAGGCC 
GAACCAGGGT CCATCGAGGA ACTACCTTGT TCTGCACTTG CTCCATCCCT AGAGCCTTGC 
TTCTCCAGGC CCGAGAGACC AGCAAACCGT CGCCCTCCGT CCCGCTGGGC CCCACACCCC 
GCCACTGCCT CACCAGCTCC ATCACCCGGA GAACCCACCT CCTTGGAGGA GCTCGGTGAT 
GAGGAGCCAC CTGGGGAGAA GCCGCACGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_024984415.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840183.1 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

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CloneID	OBa230007
Clone ID Related Accession (Same CDS sequence)	XM_024984415.1
Accession Version	XM_024984415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
AACGGAGGAG CCGAGCACCT TGGGAACTGC AGGTCTACAG CCAAAGCTCA GAGGAAACTG 
CCGCCCCGGT GCAAGGATGA CAGCGCGGAT CTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAGGGCTCGC TGATGCTCAG GAAGATGGCC AAGCTGGGGC GCGAGAAGGA CCGGCTGGAG 
CAGGAGCTGC AGAGGTACAG GGCCCTCTAC GGGGACGCTG ACAGCCCCCT GCCCACGGGC 
GAGGCCGGCG GGCCACCCAG CACCCGGGAG GCGGAGCTGA AGCTGCGGCT GAGGCTGGTG 
GAGGAGGAGG CCAGCATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAATCGGGGC 
CTCAGGGCTG AGATGGAAGA CCTGCGCGGC CGGGAATCCC TGCCCGGTGG GCCGACCTCG 
CCCTTCGGGG GTGACTCCCT GGAGTCCTCG GCTGAGCTGC GCCGGCACCT GCAGTTCGTG 
GAGGAGGAGG CGGAGCTGCT GCGGCGGTCC ATCTCCGAGA TCGAGGACCA CAACCGGCAG 
CTGACCCACG AGCTGAGCAA GTTCAAGTTC GAGCCGCGGC CCGAGCCGGG CTGGCTGGCA 
GGGGGCGGGG CGCCCCTGCA GGACGAGCTC CAGGCGGTGC GGCTGCCGAT AAGCGAGCTG 
AGCGGGGAGG TGCTGACGCT GCAGCCGGGC CCGCACGCCC CGAGCCCGCG GGACAGTGAT 
GCTGACAGCG ACACGGGCCG AAGGAGCGAC GACGGTGAGG ACGGACGCGT GCAGCCTCGC 
CGGGAGGGCC CCATTGGCGG CGAGAGTGAC TCGGAGGAGA CCTTCGAGAA GACGTCTGGC 
TTCGGAAGCG GGAGGCCGTC GGAGGCCGGC GAGTGCCCGG CGGAGCTGCT GCGCGCGCGG 
GAGGACTCGG AGCGCCTGGC GAGCATCAGG CACGAGGCGG AGCGGCTGGA GCGCACGGTG 
GAGCGCATCA TCACGGACAC CGAGGCCGCT CTCCCGGGCG GAGAGGCCTC GCCCGGGGCC 
TGCGGCCAGG GCATCAGGGA GGAGCCCCAG CTGCTGGGGA CCCTCAACGC CAGGATGAAG 
GCATTCAGGA AAGAGCTCCA GGCCTTCCTG GAGCAGGTGG AGCGACTGGG GGAGGGCCTG 
TCCCCGCTGC CCCAGCTCAC CGAGTCATCC AGCTTCCTGT CCTCTGTGAC CTCCGTATCC 
CGGGACTCCC CGGTGGGGAA CCTGGGCAAG GAGCTGGGCC CGGACCTGCA GTCCAAACTG 
AGAGATCAGA TGGAGTGGCA GCCGGGGCAG GAGCGAGGGG AGGAGCGGGG TCACCTCCAT 
CTCCGCACCG CACGGGAGCT GCACCGCCGA GCCGACGGGG ATGCCGGAAG CTGCCGGCCT 
GGAGCCCAGA GCTGCTTCAG CCTCGAGGCG GAGGAGGAAC GCCGCTGCGC CCTGCGGAGG 
CGCGACCGGG AGGCAAACGG CCTGGCCCTG CAGAGCTCGC TGCACGAGCG CGCCTGGGAC 
GACGAGAGGC ACCTGATGCA GCAGGAGCTG CGCTCCCTGA AGCAGAACAT CTTCCTCTTC 
TACGTCAAGC TCAGGTGGCT GCTCAAGCAC TGGCGCCAAG GGAGGCAGGC GGAGGACGAC 
GCGGAGGACT TCATGGAGAG CGAGCTTCCA GAGACCTTCC CCAGGCTCGG GGAGCTTGGC 
GTCCAGGGGG ACACAAAGGC GGACAGTCCA GACCGCGACG ACGATGGGCC AAGCTGTGGG 
TTTTCCCTGG GAGAGCGCTC TCCGCAGATG GGTGACCACG GGTCACGGCT GCAGCCCACC 
GACGGGGGAC AGCTCTACAG GCAGGTGGCA GAGACCCAGC CGCTGTTCTG CGCCCTCAAG 
GCACTGCTTG AGGAGCTGCG CGCAGAGCTA CGGGAGGACG CGCGCGCACG CCAGCGGCTG 
CAGCAGCAAT ACGCAGGTGA CAAGGCTGCC TGGGATGTGG AGTGGGCCGC GCTCACATGC 
CGCCTGGAGC AGCTGGAAGC AAAGAATGAA AAGAGCTTGG GAGACCCAGG CTCCTGCACC 
GACAGCAAAG GGGCATTCAG GAAGGAGAGG GAGGTGCAGC AGAAGCTGCT GGCGGACAGT 
CACAGGCTGG TCATGGACCT GCGCTGGCAG ATCCACCACA GCGAGAAGAA CTGGAACCGG 
GAGAAGGTGG AGCTTCTCGA CCGCCTGGAC CGGGAGCGCC AGGAGTGGGG GCGGCAGGAG 
AAGGAGATGC TGTGGAGAAT TGAACAGTTG CAGAAAGAAA ACAGTCCCCG GAGAGGTGGC 
AGTTTCCTCT GTGATCAAGA GGAGGGGGAC ACCCGCCCCT GTACCCACCC AGGGAGCCCT 
CGGGCGCCCC GCCCTGCGGG GATGTGGCCC TGTATGGACA CAGACTCCAG TGCGTTTGAG 
GACCGGCCGC TGTCAAAGCT GAAGGAATCG GACAGGTGCT CAGCGCGGGA GAACCTCTAC 
CTGGACACCT TGTCCCTGGA CGACGAGCCA GAGGAGCCCC CGGCCCGTGG GCCCCAGAGG 
GAGTTCAGGA ACTGCTTCCG TGAGGAAGAA GAAAATCACA AGGGAAATCT TCAAAGGGCC 
GTGTCTGTGT CGTCCATGTC TGAGTTCCAG CGCTTGATGG ATGGCTCTCC CTTCCTGCCG 
GAGAAGGGCC TGCCCCCCAC ATGTAGCAAG GAGGACGTCA CCCCACCCCT GTCCCCTGAT 
GACCTCAAGT ACATCGAGGA GTTCAACAGC AAGAGCTGGG ACGACAGGCC TCCAGACCCC 
TGGGCGGACA GGACCGAGGT GGGGCGGACG CGGCCCGAGG CCAGCACTGA ACCTTTCTCT 
GACGCCGCCT GGTACCTCAC CACAAGCATC ACCCTGACCA CGGACACCGT GACCAACCCC 
GAACACTGCC AGAAGCAGCC CCCGCGGAAC CTTGTCTGTG CTGAGCAGGC TGGGGGGAGG 
GGGCTGCACA GCCCACCCGC CATCCGGAGG CTGGAGGGCA TCATGGCGGG GGTCGGCGAG 
AGCAGGGTCC GAGGGGGCCC CGGGGATGCC AAGGGGAGCC CTTCTGAACA TGTGCTCAGC 
AGGTGGCCCT GTGCCCCCTC CAGGCACCCC CGAGACTACA CGGAGGTGGG GCTCCGGCCC 
CTCGATAGCA CCCTCAGCTT CTCCTCCCCA TTGCACAGCC TGGAAATGCC CAGAAACCTG 
AGTGATGATA TGAAGGAGGT AGCCTTCTCG GTCAGGAGCG CCATCTGCTC CAGCCCCGTT 
GAGCCTCTGG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACAAC AGGAACACAG 
ACAGTCCAGA CCATCAGTGT TGGCCTACAG ACGGAAGCCC TGCGGGGCAG CCCACACAAG 
TGTCTCACAC CCAAGGCGGG AGGTGGTGCC ACACCCGCGT CCTCTCCCTC CCGTGGCCTC 
AGGAACAGGC AGGTGGCCCC CGCCATTGAG AAGGTGCAAG CTAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTACGGGTC TCCCAAGCTG CAGCGGAAGC CCCTTCCCAG AGTGGACCAG 
TCAAGTAGCA GGGCCTCCCC GGGGGTGGCC CAGAAGGGGT GTGGCGAGTC AGCCTGGGCC 
CGCTCCACCA CCACACGCGA GAGCCCCGTG CACACCGCCA TGAACGATGG CCTCTCCAGC 
CTCTTCAGCA TCATCGACCA CAGCCCGGTG GTGCAGGACC CCTTCCAGAA GGGGGTGCGG 
ACTGCGAGTC GGTCTCGCTC GGCCGAGCCC CGGCCAGAGC TGGGTCCAGG CCAGGACACC 
AGCCCCAGCT CTCGGGGCCG CTCACCCAGC CCCATTGGGG GTGGCTCAGA GACATGCAGG 
GAGGAGGGGG GAGAGGGCAC GCCCCTGAGG CAGGACTTAT CTGCTCCCCC TGGCTACACA 
CTCACCGAGA ACGTGGCCCG GATCCTCCAC AGAAAGCTGT TGGAACATGC CTTAAAGGAG 
GAGCGGAGGC AGGCTGCCCA CAGCCCCACA AATAGCAGCA GCGACAACCA CATGGGAGAA 
GCGGTGGTCA AGGCCGAACC AGGGTCCATC GAGGAACTAC CTTGTTCTGC ACTTGCTCCA 
TCCCTAGAGC CTTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGC 
TGGGCCCCAC ACCCCGCCAC TGCCTCACCA GCTCCATCAC CCGGAGAACC CACCTCCTTG 
GAGGAGCTCG GTGATGAGGA GCCACCTGGG GAGAAGCCGC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840183.1
CDS	581..5308
Translation	MEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQNGGAEHLGNCRSTAKAQRKLPPRCKDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHPATASPAPSPGEPTSLEELGDEEPPGEKPHV

Target ORF information:

RefSeq Version	XM_024984415.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984415.1

ORF Insert Sequence:

ATGGAGGAGC TGCGCTCGGA GAACGACTAT CTCAAGGATG AGTTGGATGA ACTCCGAGCG 
GAAATGGAAG AGATGAGAGA CAGTTACTTG GAGGAGGAGG TTTACCAGCT GCAGGAGCTG 
CGGCGGGAGC TGGACCGCGC CAACAAGAAC TGCCGAATCC TGCAGTACCG CCTCCGGAAG 
GCCGAGCAGA AGAGGCTGAA GGTGGCGGAG ACCGGGCAGG TGGACGGGGA GCTTATCCGG 
AGTCTGGAGC AGGACCTGAA GGTGGCCAAA GATGTATCGG TCCGATTGCA CCATGAACTT 
GAAACCGTGG AGGAAAAGAG AGCTAAAGCG GAAGACGAGA ATGAAACTCT CCGACAGCAG 
ATGATCGAAG TGGAGATATC AAAGCAGGCC CTGCAGAACG AGCTGGAGAG ACTGAAGGAG 
AGTTCCTTGA AGAGAAGAGG CAGCCGGGAA GCGTACAAAG AGAAGAAAGC CTTCACCCAG 
AACGGAGGAG CCGAGCACCT TGGGAACTGC AGGTCTACAG CCAAAGCTCA GAGGAAACTG 
CCGCCCCGGT GCAAGGATGA CAGCGCGGAT CTGAGGTGCC AGCTCCAGTT TGCCAAAGAG 
GAGGGCTCGC TGATGCTCAG GAAGATGGCC AAGCTGGGGC GCGAGAAGGA CCGGCTGGAG 
CAGGAGCTGC AGAGGTACAG GGCCCTCTAC GGGGACGCTG ACAGCCCCCT GCCCACGGGC 
GAGGCCGGCG GGCCACCCAG CACCCGGGAG GCGGAGCTGA AGCTGCGGCT GAGGCTGGTG 
GAGGAGGAGG CCAGCATCTT GGGCCGGAAG ATCGTGGAGC TGGAGGTGGA GAATCGGGGC 
CTCAGGGCTG AGATGGAAGA CCTGCGCGGC CGGGAATCCC TGCCCGGTGG GCCGACCTCG 
CCCTTCGGGG GTGACTCCCT GGAGTCCTCG GCTGAGCTGC GCCGGCACCT GCAGTTCGTG 
GAGGAGGAGG CGGAGCTGCT GCGGCGGTCC ATCTCCGAGA TCGAGGACCA CAACCGGCAG 
CTGACCCACG AGCTGAGCAA GTTCAAGTTC GAGCCGCGGC CCGAGCCGGG CTGGCTGGCA 
GGGGGCGGGG CGCCCCTGCA GGACGAGCTC CAGGCGGTGC GGCTGCCGAT AAGCGAGCTG 
AGCGGGGAGG TGCTGACGCT GCAGCCGGGC CCGCACGCCC CGAGCCCGCG GGACAGTGAT 
GCTGACAGCG ACACGGGCCG AAGGAGCGAC GACGGTGAGG ACGGACGCGT GCAGCCTCGC 
CGGGAGGGCC CCATTGGCGG CGAGAGTGAC TCGGAGGAGA CCTTCGAGAA GACGTCTGGC 
TTCGGAAGCG GGAGGCCGTC GGAGGCCGGC GAGTGCCCGG CGGAGCTGCT GCGCGCGCGG 
GAGGACTCGG AGCGCCTGGC GAGCATCAGG CACGAGGCGG AGCGGCTGGA GCGCACGGTG 
GAGCGCATCA TCACGGACAC CGAGGCCGCT CTCCCGGGCG GAGAGGCCTC GCCCGGGGCC 
TGCGGCCAGG GCATCAGGGA GGAGCCCCAG CTGCTGGGGA CCCTCAACGC CAGGATGAAG 
GCATTCAGGA AAGAGCTCCA GGCCTTCCTG GAGCAGGTGG AGCGACTGGG GGAGGGCCTG 
TCCCCGCTGC CCCAGCTCAC CGAGTCATCC AGCTTCCTGT CCTCTGTGAC CTCCGTATCC 
CGGGACTCCC CGGTGGGGAA CCTGGGCAAG GAGCTGGGCC CGGACCTGCA GTCCAAACTG 
AGAGATCAGA TGGAGTGGCA GCCGGGGCAG GAGCGAGGGG AGGAGCGGGG TCACCTCCAT 
CTCCGCACCG CACGGGAGCT GCACCGCCGA GCCGACGGGG ATGCCGGAAG CTGCCGGCCT 
GGAGCCCAGA GCTGCTTCAG CCTCGAGGCG GAGGAGGAAC GCCGCTGCGC CCTGCGGAGG 
CGCGACCGGG AGGCAAACGG CCTGGCCCTG CAGAGCTCGC TGCACGAGCG CGCCTGGGAC 
GACGAGAGGC ACCTGATGCA GCAGGAGCTG CGCTCCCTGA AGCAGAACAT CTTCCTCTTC 
TACGTCAAGC TCAGGTGGCT GCTCAAGCAC TGGCGCCAAG GGAGGCAGGC GGAGGACGAC 
GCGGAGGACT TCATGGAGAG CGAGCTTCCA GAGACCTTCC CCAGGCTCGG GGAGCTTGGC 
GTCCAGGGGG ACACAAAGGC GGACAGTCCA GACCGCGACG ACGATGGGCC AAGCTGTGGG 
TTTTCCCTGG GAGAGCGCTC TCCGCAGATG GGTGACCACG GGTCACGGCT GCAGCCCACC 
GACGGGGGAC AGCTCTACAG GCAGGTGGCA GAGACCCAGC CGCTGTTCTG CGCCCTCAAG 
GCACTGCTTG AGGAGCTGCG CGCAGAGCTA CGGGAGGACG CGCGCGCACG CCAGCGGCTG 
CAGCAGCAAT ACGCAGGTGA CAAGGCTGCC TGGGATGTGG AGTGGGCCGC GCTCACATGC 
CGCCTGGAGC AGCTGGAAGC AAAGAATGAA AAGAGCTTGG GAGACCCAGG CTCCTGCACC 
GACAGCAAAG GGGCATTCAG GAAGGAGAGG GAGGTGCAGC AGAAGCTGCT GGCGGACAGT 
CACAGGCTGG TCATGGACCT GCGCTGGCAG ATCCACCACA GCGAGAAGAA CTGGAACCGG 
GAGAAGGTGG AGCTTCTCGA CCGCCTGGAC CGGGAGCGCC AGGAGTGGGG GCGGCAGGAG 
AAGGAGATGC TGTGGAGAAT TGAACAGTTG CAGAAAGAAA ACAGTCCCCG GAGAGGTGGC 
AGTTTCCTCT GTGATCAAGA GGAGGGGGAC ACCCGCCCCT GTACCCACCC AGGGAGCCCT 
CGGGCGCCCC GCCCTGCGGG GATGTGGCCC TGTATGGACA CAGACTCCAG TGCGTTTGAG 
GACCGGCCGC TGTCAAAGCT GAAGGAATCG GACAGGTGCT CAGCGCGGGA GAACCTCTAC 
CTGGACACCT TGTCCCTGGA CGACGAGCCA GAGGAGCCCC CGGCCCGTGG GCCCCAGAGG 
GAGTTCAGGA ACTGCTTCCG TGAGGAAGAA GAAAATCACA AGGGAAATCT TCAAAGGGCC 
GTGTCTGTGT CGTCCATGTC TGAGTTCCAG CGCTTGATGG ATGGCTCTCC CTTCCTGCCG 
GAGAAGGGCC TGCCCCCCAC ATGTAGCAAG GAGGACGTCA CCCCACCCCT GTCCCCTGAT 
GACCTCAAGT ACATCGAGGA GTTCAACAGC AAGAGCTGGG ACGACAGGCC TCCAGACCCC 
TGGGCGGACA GGACCGAGGT GGGGCGGACG CGGCCCGAGG CCAGCACTGA ACCTTTCTCT 
GACGCCGCCT GGTACCTCAC CACAAGCATC ACCCTGACCA CGGACACCGT GACCAACCCC 
GAACACTGCC AGAAGCAGCC CCCGCGGAAC CTTGTCTGTG CTGAGCAGGC TGGGGGGAGG 
GGGCTGCACA GCCCACCCGC CATCCGGAGG CTGGAGGGCA TCATGGCGGG GGTCGGCGAG 
AGCAGGGTCC GAGGGGGCCC CGGGGATGCC AAGGGGAGCC CTTCTGAACA TGTGCTCAGC 
AGGTGGCCCT GTGCCCCCTC CAGGCACCCC CGAGACTACA CGGAGGTGGG GCTCCGGCCC 
CTCGATAGCA CCCTCAGCTT CTCCTCCCCA TTGCACAGCC TGGAAATGCC CAGAAACCTG 
AGTGATGATA TGAAGGAGGT AGCCTTCTCG GTCAGGAGCG CCATCTGCTC CAGCCCCGTT 
GAGCCTCTGG TCAAGGACAT GGCCTGCCAG ACCAATGGGT CCCGGACAAC AGGAACACAG 
ACAGTCCAGA CCATCAGTGT TGGCCTACAG ACGGAAGCCC TGCGGGGCAG CCCACACAAG 
TGTCTCACAC CCAAGGCGGG AGGTGGTGCC ACACCCGCGT CCTCTCCCTC CCGTGGCCTC 
AGGAACAGGC AGGTGGCCCC CGCCATTGAG AAGGTGCAAG CTAAGTTTGA ACGCACATGC 
TGCTCCCCCA AGTACGGGTC TCCCAAGCTG CAGCGGAAGC CCCTTCCCAG AGTGGACCAG 
TCAAGTAGCA GGGCCTCCCC GGGGGTGGCC CAGAAGGGGT GTGGCGAGTC AGCCTGGGCC 
CGCTCCACCA CCACACGCGA GAGCCCCGTG CACACCGCCA TGAACGATGG CCTCTCCAGC 
CTCTTCAGCA TCATCGACCA CAGCCCGGTG GTGCAGGACC CCTTCCAGAA GGGGGTGCGG 
ACTGCGAGTC GGTCTCGCTC GGCCGAGCCC CGGCCAGAGC TGGGTCCAGG CCAGGACACC 
AGCCCCAGCT CTCGGGGCCG CTCACCCAGC CCCATTGGGG GTGGCTCAGA GACATGCAGG 
GAGGAGGGGG GAGAGGGCAC GCCCCTGAGG CAGGACTTAT CTGCTCCCCC TGGCTACACA 
CTCACCGAGA ACGTGGCCCG GATCCTCCAC AGAAAGCTGT TGGAACATGC CTTAAAGGAG 
GAGCGGAGGC AGGCTGCCCA CAGCCCCACA AATAGCAGCA GCGACAACCA CATGGGAGAA 
GCGGTGGTCA AGGCCGAACC AGGGTCCATC GAGGAACTAC CTTGTTCTGC ACTTGCTCCA 
TCCCTAGAGC CTTGCTTCTC CAGGCCCGAG AGACCAGCAA ACCGTCGCCC TCCGTCCCGC 
TGGGCCCCAC ACCCCGCCAC TGCCTCACCA GCTCCATCAC CCGGAGAACC CACCTCCTTG 
GAGGAGCTCG GTGATGAGGA GCCACCTGGG GAGAAGCCGC ACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_005224298.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224355.1 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

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CloneID	OBa32643
Clone ID Related Accession (Same CDS sequence)	XM_005224298.3 , XM_010825558.2
Accession Version	XM_005224298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGGAACT ACCTTGTTCT GCACTTGCTC CATCCCTAGA GCCTTGCTTC 
TCCAGGCCCG AGAGACCAGC AAACCGTCGC CCTCCGTCCC GCTGGGCCCC ACACCCCGCC 
ACTGCCTCAC CAGCTCCATC ACCCGGAGAA CCCACCTCCT TGGAGGAGCT CGGTGATGAG 
GAGCCACCTG GGGAGAAGCC GCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224355.1
CDS	64..5733
Translation	METLNGPAGGGAQDPKPQPPGQHHRQHHHLHPLAERRRLHRAPSPARPFLKDLHGRPAAPGQAAPSSGRAPAPAAPRSPSLAGKAPPSPGPPAAPGRLSRRSGGAPGAKDKPPPGAGARAAGGAKAAPGPRRVARAAPAEPLPRVGKPPPGAEPPPAAAKGRRAKRASGAVPARAAGPRVPAVAVPAVILAVTSAAGSPAPGSRISHTDSSSDLSDCPSDPLSDEQRLLPAASSDAESGTGSSDREPLRGAPTTSPPARGPPPDSPEPPGLLAATPAACLGGRNSPSGVPSASPGPGVVEDVAGRAPPERTGPATPREHGLGEQHRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHPATASPAPSPGEPTSLEELGDEEPPGEKPHV

Target ORF information:

RefSeq Version	XM_005224298.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224298.3

ORF Insert Sequence:

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGGAACT ACCTTGTTCT GCACTTGCTC CATCCCTAGA GCCTTGCTTC 
TCCAGGCCCG AGAGACCAGC AAACCGTCGC CCTCCGTCCC GCTGGGCCCC ACACCCCGCC 
ACTGCCTCAC CAGCTCCATC ACCCGGAGAA CCCACCTCCT TGGAGGAGCT CGGTGATGAG 
GAGCCACCTG GGGAGAAGCC GCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_010825558.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010823860.2 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

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CloneID	OBa32643
Clone ID Related Accession (Same CDS sequence)	XM_005224298.3 , XM_010825558.2
Accession Version	XM_010825558.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010825558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGGAACT ACCTTGTTCT GCACTTGCTC CATCCCTAGA GCCTTGCTTC 
TCCAGGCCCG AGAGACCAGC AAACCGTCGC CCTCCGTCCC GCTGGGCCCC ACACCCCGCC 
ACTGCCTCAC CAGCTCCATC ACCCGGAGAA CCCACCTCCT TGGAGGAGCT CGGTGATGAG 
GAGCCACCTG GGGAGAAGCC GCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010823860.2
CDS	64..5733
Translation	METLNGPAGGGAQDPKPQPPGQHHRQHHHLHPLAERRRLHRAPSPARPFLKDLHGRPAAPGQAAPSSGRAPAPAAPRSPSLAGKAPPSPGPPAAPGRLSRRSGGAPGAKDKPPPGAGARAAGGAKAAPGPRRVARAAPAEPLPRVGKPPPGAEPPPAAAKGRRAKRASGAVPARAAGPRVPAVAVPAVILAVTSAAGSPAPGSRISHTDSSSDLSDCPSDPLSDEQRLLPAASSDAESGTGSSDREPLRGAPTTSPPARGPPPDSPEPPGLLAATPAACLGGRNSPSGVPSASPGPGVVEDVAGRAPPERTGPATPREHGLGEQHRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIEELPCSALAPSLEPCFSRPERPANRRPPSRWAPHPATASPAPSPGEPTSLEELGDEEPPGEKPHV

Target ORF information:

RefSeq Version	XM_010825558.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010825558.2

ORF Insert Sequence:

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGGAACT ACCTTGTTCT GCACTTGCTC CATCCCTAGA GCCTTGCTTC 
TCCAGGCCCG AGAGACCAGC AAACCGTCGC CCTCCGTCCC GCTGGGCCCC ACACCCCGCC 
ACTGCCTCAC CAGCTCCATC ACCCGGAGAA CCCACCTCCT TGGAGGAGCT CGGTGATGAG 
GAGCCACCTG GGGAGAAGCC GCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_015469149.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324635.1 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

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CloneID	OBa32645
Clone ID Related Accession (Same CDS sequence)	XM_015469149.1 , XM_010818953.2
Accession Version	XM_015469149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646636.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGAACCA AACTGTCTTA CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324635.1
CDS	64..5577
Translation	METLNGPAGGGAQDPKPQPPGQHHRQHHHLHPLAERRRLHRAPSPARPFLKDLHGRPAAPGQAAPSSGRAPAPAAPRSPSLAGKAPPSPGPPAAPGRLSRRSGGAPGAKDKPPPGAGARAAGGAKAAPGPRRVARAAPAEPLPRVGKPPPGAEPPPAAAKGRRAKRASGAVPARAAGPRVPAVAVPAVILAVTSAAGSPAPGSRISHTDSSSDLSDCPSDPLSDEQRLLPAASSDAESGTGSSDREPLRGAPTTSPPARGPPPDSPEPPGLLAATPAACLGGRNSPSGVPSASPGPGVVEDVAGRAPPERTGPATPREHGLGEQHRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_015469149.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469149.1

ORF Insert Sequence:

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGAACCA AACTGTCTTA CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTCL1 ( XM_010818953.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817255.1 Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

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CloneID	OBa32645
Clone ID Related Accession (Same CDS sequence)	XM_015469149.1 , XM_010818953.2
Accession Version	XM_010818953.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	microtubule cross-linking factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818953.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGAACCA AACTGTCTTA CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817255.1
CDS	64..5577
Translation	METLNGPAGGGAQDPKPQPPGQHHRQHHHLHPLAERRRLHRAPSPARPFLKDLHGRPAAPGQAAPSSGRAPAPAAPRSPSLAGKAPPSPGPPAAPGRLSRRSGGAPGAKDKPPPGAGARAAGGAKAAPGPRRVARAAPAEPLPRVGKPPPGAEPPPAAAKGRRAKRASGAVPARAAGPRVPAVAVPAVILAVTSAAGSPAPGSRISHTDSSSDLSDCPSDPLSDEQRLLPAASSDAESGTGSSDREPLRGAPTTSPPARGPPPDSPEPPGLLAATPAACLGGRNSPSGVPSASPGPGVVEDVAGRAPPERTGPATPREHGLGEQHRLVPAAEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEEVYQLQELRRELDRANKNCRILQYRLRKAEQKRLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENETLRQQMIEVEISKQALQNELERLKESSLKRRGSREAYKEKKAFTQDDSADLRCQLQFAKEEGSLMLRKMAKLGREKDRLEQELQRYRALYGDADSPLPTGEAGGPPSTREAELKLRLRLVEEEASILGRKIVELEVENRGLRAEMEDLRGRESLPGGPTSPFGGDSLESSAELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFEPRPEPGWLAGGGAPLQDELQAVRLPISELSGEVLTLQPGPHAPSPRDSDADSDTGRRSDDGEDGRVQPRREGPIGGESDSEETFEKTSGFGSGRPSEAGECPAELLRAREDSERLASIRHEAERLERTVERIITDTEAALPGGEASPGACGQGIREEPQLLGTLNARMKAFRKELQAFLEQVERLGEGLSPLPQLTESSSFLSSVTSVSRDSPVGNLGKELGPDLQSKLRDQMEWQPGQERGEERGHLHLRTARELHRRADGDAGSCRPGAQSCFSLEAEEERRCALRRRDREANGLALQSSLHERAWDDERHLMQQELRSLKQNIFLFYVKLRWLLKHWRQGRQAEDDAEDFMESELPETFPRLGELGVQGDTKADSPDRDDDGPSCGFSLGERSPQMGDHGSRLQPTDGGQLYRQVAETQPLFCALKALLEELRAELREDARARQRLQQQYAGDKAAWDVEWAALTCRLEQLEAKNEKSLGDPGSCTDSKGAFRKEREVQQKLLADSHRLVMDLRWQIHHSEKNWNREKVELLDRLDRERQEWGRQEKEMLWRIEQLQKENSPRRGGSFLCDQEEGDTRPCTHPGSPRAPRPAGMWPCMDTDSSAFEDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFRNCFREEEENHKGNLQRAVSVSSMSEFQRLMDGSPFLPEKGLPPTCSKEDVTPPLSPDDLKYIEEFNSKSWDDRPPDPWADRTEVGRTRPEASTEPFSDAAWYLTTSITLTTDTVTNPEHCQKQPPRNLVCAEQAGGRGLHSPPAIRRLEGIMAGVGESRVRGGPGDAKGSPSEHVLSRWPCAPSRHPRDYTEVGLRPLDSTLSFSSPLHSLEMPRNLSDDMKEVAFSVRSAICSSPVEPLVKDMACQTNGSRTTGTQTVQTISVGLQTEALRGSPHKCLTPKAGGGATPASSPSRGLRNRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPRVDQSSSRASPGVAQKGCGESAWARSTTTRESPVHTAMNDGLSSLFSIIDHSPVVQDPFQKGVRTASRSRSAEPRPELGPGQDTSPSSRGRSPSPIGGGSETCREEGGEGTPLRQDLSAPPGYTLTENVARILHRKLLEHALKEERRQAAHSPTNSSSDNHMGEAVVKAEPGSIENQTVLLTAPWGL

Target ORF information:

RefSeq Version	XM_010818953.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule crosslinking factor 1 (MTCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818953.2

ORF Insert Sequence:

ATGGAGACGC TGAACGGCCC CGCGGGCGGC GGCGCCCAGG ACCCGAAGCC GCAGCCGCCC 
GGCCAGCACC ACCGCCAGCA CCACCACCTC CACCCGCTGG CGGAACGGAG ACGGCTGCAC 
CGCGCACCCT CGCCAGCCAG ACCGTTTCTC AAGGACCTGC ACGGCCGGCC TGCGGCCCCC 
GGCCAGGCCG CCCCCTCCTC GGGCCGAGCC CCGGCGCCCG CCGCCCCGCG CTCTCCCAGC 
CTCGCGGGCA AGGCGCCGCC CTCGCCGGGG CCCCCGGCCG CGCCCGGCCG CCTCTCCCGG 
CGGAGCGGTG GCGCTCCCGG CGCGAAGGAC AAGCCGCCGC CGGGCGCCGG GGCCAGGGCA 
GCGGGCGGCG CTAAGGCCGC TCCGGGCCCC AGGAGGGTGG CACGCGCGGC GCCCGCCGAG 
CCGCTGCCCC GGGTAGGGAA GCCACCGCCC GGGGCCGAGC CGCCGCCCGC CGCTGCCAAG 
GGCCGCAGAG CCAAACGCGC CTCCGGAGCT GTCCCCGCCC GCGCCGCCGG GCCCCGGGTC 
CCCGCCGTCG CGGTCCCGGC CGTGATCCTC GCCGTGACTT CCGCGGCCGG CTCCCCGGCC 
CCCGGCTCGC GCATCAGCCA CACAGACAGC AGCTCCGATC TCTCCGACTG CCCCTCCGAC 
CCTCTTTCCG ACGAGCAGCG CCTGCTCCCC GCCGCCAGTA GTGACGCCGA GTCCGGCACG 
GGCTCCAGCG ACCGGGAACC TCTGCGAGGA GCCCCCACGA CCAGCCCCCC AGCTCGGGGG 
CCGCCGCCAG ACAGCCCCGA GCCCCCGGGA CTCCTCGCCG CGACCCCCGC CGCCTGCCTT 
GGGGGTCGGA ACAGCCCCAG TGGGGTCCCC TCGGCGTCCC CGGGGCCGGG CGTGGTGGAG 
GATGTCGCGG GGCGCGCACC GCCGGAGCGA ACGGGTCCCG CGACCCCCAG GGAGCACGGG 
CTGGGCGAGC AGCACCGGTT GGTCCCGGCG GCGGAGGAGG AGGAGCTGCT GCGGGAGATG 
GAGGAGCTGC GCTCGGAGAA CGACTATCTC AAGGATGAGT TGGATGAACT CCGAGCGGAA 
ATGGAAGAGA TGAGAGACAG TTACTTGGAG GAGGAGGTTT ACCAGCTGCA GGAGCTGCGG 
CGGGAGCTGG ACCGCGCCAA CAAGAACTGC CGAATCCTGC AGTACCGCCT CCGGAAGGCC 
GAGCAGAAGA GGCTGAAGGT GGCGGAGACC GGGCAGGTGG ACGGGGAGCT TATCCGGAGT 
CTGGAGCAGG ACCTGAAGGT GGCCAAAGAT GTATCGGTCC GATTGCACCA TGAACTTGAA 
ACCGTGGAGG AAAAGAGAGC TAAAGCGGAA GACGAGAATG AAACTCTCCG ACAGCAGATG 
ATCGAAGTGG AGATATCAAA GCAGGCCCTG CAGAACGAGC TGGAGAGACT GAAGGAGAGT 
TCCTTGAAGA GAAGAGGCAG CCGGGAAGCG TACAAAGAGA AGAAAGCCTT CACCCAGGAT 
GACAGCGCGG ATCTGAGGTG CCAGCTCCAG TTTGCCAAAG AGGAGGGCTC GCTGATGCTC 
AGGAAGATGG CCAAGCTGGG GCGCGAGAAG GACCGGCTGG AGCAGGAGCT GCAGAGGTAC 
AGGGCCCTCT ACGGGGACGC TGACAGCCCC CTGCCCACGG GCGAGGCCGG CGGGCCACCC 
AGCACCCGGG AGGCGGAGCT GAAGCTGCGG CTGAGGCTGG TGGAGGAGGA GGCCAGCATC 
TTGGGCCGGA AGATCGTGGA GCTGGAGGTG GAGAATCGGG GCCTCAGGGC TGAGATGGAA 
GACCTGCGCG GCCGGGAATC CCTGCCCGGT GGGCCGACCT CGCCCTTCGG GGGTGACTCC 
CTGGAGTCCT CGGCTGAGCT GCGCCGGCAC CTGCAGTTCG TGGAGGAGGA GGCGGAGCTG 
CTGCGGCGGT CCATCTCCGA GATCGAGGAC CACAACCGGC AGCTGACCCA CGAGCTGAGC 
AAGTTCAAGT TCGAGCCGCG GCCCGAGCCG GGCTGGCTGG CAGGGGGCGG GGCGCCCCTG 
CAGGACGAGC TCCAGGCGGT GCGGCTGCCG ATAAGCGAGC TGAGCGGGGA GGTGCTGACG 
CTGCAGCCGG GCCCGCACGC CCCGAGCCCG CGGGACAGTG ATGCTGACAG CGACACGGGC 
CGAAGGAGCG ACGACGGTGA GGACGGACGC GTGCAGCCTC GCCGGGAGGG CCCCATTGGC 
GGCGAGAGTG ACTCGGAGGA GACCTTCGAG AAGACGTCTG GCTTCGGAAG CGGGAGGCCG 
TCGGAGGCCG GCGAGTGCCC GGCGGAGCTG CTGCGCGCGC GGGAGGACTC GGAGCGCCTG 
GCGAGCATCA GGCACGAGGC GGAGCGGCTG GAGCGCACGG TGGAGCGCAT CATCACGGAC 
ACCGAGGCCG CTCTCCCGGG CGGAGAGGCC TCGCCCGGGG CCTGCGGCCA GGGCATCAGG 
GAGGAGCCCC AGCTGCTGGG GACCCTCAAC GCCAGGATGA AGGCATTCAG GAAAGAGCTC 
CAGGCCTTCC TGGAGCAGGT GGAGCGACTG GGGGAGGGCC TGTCCCCGCT GCCCCAGCTC 
ACCGAGTCAT CCAGCTTCCT GTCCTCTGTG ACCTCCGTAT CCCGGGACTC CCCGGTGGGG 
AACCTGGGCA AGGAGCTGGG CCCGGACCTG CAGTCCAAAC TGAGAGATCA GATGGAGTGG 
CAGCCGGGGC AGGAGCGAGG GGAGGAGCGG GGTCACCTCC ATCTCCGCAC CGCACGGGAG 
CTGCACCGCC GAGCCGACGG GGATGCCGGA AGCTGCCGGC CTGGAGCCCA GAGCTGCTTC 
AGCCTCGAGG CGGAGGAGGA ACGCCGCTGC GCCCTGCGGA GGCGCGACCG GGAGGCAAAC 
GGCCTGGCCC TGCAGAGCTC GCTGCACGAG CGCGCCTGGG ACGACGAGAG GCACCTGATG 
CAGCAGGAGC TGCGCTCCCT GAAGCAGAAC ATCTTCCTCT TCTACGTCAA GCTCAGGTGG 
CTGCTCAAGC ACTGGCGCCA AGGGAGGCAG GCGGAGGACG ACGCGGAGGA CTTCATGGAG 
AGCGAGCTTC CAGAGACCTT CCCCAGGCTC GGGGAGCTTG GCGTCCAGGG GGACACAAAG 
GCGGACAGTC CAGACCGCGA CGACGATGGG CCAAGCTGTG GGTTTTCCCT GGGAGAGCGC 
TCTCCGCAGA TGGGTGACCA CGGGTCACGG CTGCAGCCCA CCGACGGGGG ACAGCTCTAC 
AGGCAGGTGG CAGAGACCCA GCCGCTGTTC TGCGCCCTCA AGGCACTGCT TGAGGAGCTG 
CGCGCAGAGC TACGGGAGGA CGCGCGCGCA CGCCAGCGGC TGCAGCAGCA ATACGCAGGT 
GACAAGGCTG CCTGGGATGT GGAGTGGGCC GCGCTCACAT GCCGCCTGGA GCAGCTGGAA 
GCAAAGAATG AAAAGAGCTT GGGAGACCCA GGCTCCTGCA CCGACAGCAA AGGGGCATTC 
AGGAAGGAGA GGGAGGTGCA GCAGAAGCTG CTGGCGGACA GTCACAGGCT GGTCATGGAC 
CTGCGCTGGC AGATCCACCA CAGCGAGAAG AACTGGAACC GGGAGAAGGT GGAGCTTCTC 
GACCGCCTGG ACCGGGAGCG CCAGGAGTGG GGGCGGCAGG AGAAGGAGAT GCTGTGGAGA 
ATTGAACAGT TGCAGAAAGA AAACAGTCCC CGGAGAGGTG GCAGTTTCCT CTGTGATCAA 
GAGGAGGGGG ACACCCGCCC CTGTACCCAC CCAGGGAGCC CTCGGGCGCC CCGCCCTGCG 
GGGATGTGGC CCTGTATGGA CACAGACTCC AGTGCGTTTG AGGACCGGCC GCTGTCAAAG 
CTGAAGGAAT CGGACAGGTG CTCAGCGCGG GAGAACCTCT ACCTGGACAC CTTGTCCCTG 
GACGACGAGC CAGAGGAGCC CCCGGCCCGT GGGCCCCAGA GGGAGTTCAG GAACTGCTTC 
CGTGAGGAAG AAGAAAATCA CAAGGGAAAT CTTCAAAGGG CCGTGTCTGT GTCGTCCATG 
TCTGAGTTCC AGCGCTTGAT GGATGGCTCT CCCTTCCTGC CGGAGAAGGG CCTGCCCCCC 
ACATGTAGCA AGGAGGACGT CACCCCACCC CTGTCCCCTG ATGACCTCAA GTACATCGAG 
GAGTTCAACA GCAAGAGCTG GGACGACAGG CCTCCAGACC CCTGGGCGGA CAGGACCGAG 
GTGGGGCGGA CGCGGCCCGA GGCCAGCACT GAACCTTTCT CTGACGCCGC CTGGTACCTC 
ACCACAAGCA TCACCCTGAC CACGGACACC GTGACCAACC CCGAACACTG CCAGAAGCAG 
CCCCCGCGGA ACCTTGTCTG TGCTGAGCAG GCTGGGGGGA GGGGGCTGCA CAGCCCACCC 
GCCATCCGGA GGCTGGAGGG CATCATGGCG GGGGTCGGCG AGAGCAGGGT CCGAGGGGGC 
CCCGGGGATG CCAAGGGGAG CCCTTCTGAA CATGTGCTCA GCAGGTGGCC CTGTGCCCCC 
TCCAGGCACC CCCGAGACTA CACGGAGGTG GGGCTCCGGC CCCTCGATAG CACCCTCAGC 
TTCTCCTCCC CATTGCACAG CCTGGAAATG CCCAGAAACC TGAGTGATGA TATGAAGGAG 
GTAGCCTTCT CGGTCAGGAG CGCCATCTGC TCCAGCCCCG TTGAGCCTCT GGTCAAGGAC 
ATGGCCTGCC AGACCAATGG GTCCCGGACA ACAGGAACAC AGACAGTCCA GACCATCAGT 
GTTGGCCTAC AGACGGAAGC CCTGCGGGGC AGCCCACACA AGTGTCTCAC ACCCAAGGCG 
GGAGGTGGTG CCACACCCGC GTCCTCTCCC TCCCGTGGCC TCAGGAACAG GCAGGTGGCC 
CCCGCCATTG AGAAGGTGCA AGCTAAGTTT GAACGCACAT GCTGCTCCCC CAAGTACGGG 
TCTCCCAAGC TGCAGCGGAA GCCCCTTCCC AGAGTGGACC AGTCAAGTAG CAGGGCCTCC 
CCGGGGGTGG CCCAGAAGGG GTGTGGCGAG TCAGCCTGGG CCCGCTCCAC CACCACACGC 
GAGAGCCCCG TGCACACCGC CATGAACGAT GGCCTCTCCA GCCTCTTCAG CATCATCGAC 
CACAGCCCGG TGGTGCAGGA CCCCTTCCAG AAGGGGGTGC GGACTGCGAG TCGGTCTCGC 
TCGGCCGAGC CCCGGCCAGA GCTGGGTCCA GGCCAGGACA CCAGCCCCAG CTCTCGGGGC 
CGCTCACCCA GCCCCATTGG GGGTGGCTCA GAGACATGCA GGGAGGAGGG GGGAGAGGGC 
ACGCCCCTGA GGCAGGACTT ATCTGCTCCC CCTGGCTACA CACTCACCGA GAACGTGGCC 
CGGATCCTCC ACAGAAAGCT GTTGGAACAT GCCTTAAAGG AGGAGCGGAG GCAGGCTGCC 
CACAGCCCCA CAAATAGCAG CAGCGACAAC CACATGGGAG AAGCGGTGGT CAAGGCCGAA 
CCAGGGTCCA TCGAGAACCA AACTGTCTTA CTAACTGCCC CCTGGGGACT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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電話

03-6811-6572

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050-3174-7589(受注専用)

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オンライン注文

MTCL1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム