GYS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GYS2
Entrez Gene ID	537451
Full Name	glycogen synthase 2
General protein information	Preferred Names glycogen synthase 2 Names glycogen [starch] synthase, liver glycogen synthase 2 (liver)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005206956.3	XP_005207013.1	glycogen [starch] synthase, liver isoform X1
XM_015471237.1	XP_015326723.1	glycogen [starch] synthase, liver isoform X3
NM_001192905.1	NP_001179834.1	glycogen [starch] synthase, liver
XM_010805421.2	XP_010803723.1	glycogen [starch] synthase, liver isoform X2
NM_001192905.2	NP_001179834.2	glycogen [starch] synthase, liver

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GYS2	NP_001179834.1
Mus musculus (house mouse)	Gys2	NP_663547.2
Danio rerio (zebrafish)	gys2	NP_001018199.1
Pan troglodytes (chimpanzee)	GYS2	XP_520790.2
Gallus gallus (chicken)	GYS2	XP_004938048.1
Canis lupus familiaris (dog)	GYS2	XP_534869.2
Homo sapiens (human)	GYS2	NP_068776.2
	GYS2	XP_001098578.1
Rattus norvegicus (Norway rat)	Gys2	NP_037221.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GYS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GYS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa37284	NM_001192905.1 Latest version!	Bos taurus glycogen synthase 2 (GYS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19664	XM_005206956.3 Latest version!	Bos taurus glycogen synthase 2 (GYS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa53654	XM_010805421.2 Latest version!	Bos taurus glycogen synthase 2 (GYS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa239153	NM_001192905.2 Latest version!	Bos taurus glycogen synthase 2 (GYS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa90796	XM_015471237.1 Latest version!	Bos taurus glycogen synthase 2 (GYS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GYS2 ( NM_001192905.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179834.1 Bos taurus glycogen synthase 2 (GYS2), mRNA.

Price & Availability↑

CloneID	OBa37284
Clone ID Related Accession (Same CDS sequence)	NM_001192905.1
Accession Version	NM_001192905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	glycogen [starch] synthase, liver
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02014121.1. On Jul 17, 2010 this sequence version replaced XM_617616.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822095 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGT TGATTTTGCA CCCAGAGTTC 
CTGTCCTCCA CCAGCCCCTT GCTACCCATG GATTATGAAG AGTTTGTCCG AGGTTGTCAT 
CTTGGAGTAT TTCCATCTTA CTATGAACCC TGGGGTTATA CTCCAGCTGA ATGCACTGTG 
ATGGGCATCC CCAGTGTGAC CACCAATCTC TCTGGCTTTG GCTGTTTCAT GCAGGAACAT 
GTGGCTGACC CCACTGCATA CGGAATTTAC ATCGTTGACA GGCGGTTCTG CTCACCCGAT 
GATTCTTGCA ATCAGCTGAC TCAGTTTCTC TATGGATTTT GCAAACAGTC ACGCCGCCAG 
AGGATTATTC AAAGGAATCG AACCGAGAGG CTCTCAGATC TTCTGGACTG GAGATACTTA 
GGCAGATATT ACCAGCATGC CAGGCACCTG ACATTAAGCA GAGCCTTTCC AGAAAAATTC 
CACATGGAAC CCACATCACC ACCAACGACA GAAGGGTTTA AGTATCCCAG GCCGCCCTCG 
GTACCACCTT CTCCGTCGGG ATCTCAGGGC TCCAGTCCTC AGAGCAGCGA TGCGGAGGAT 
GACGATGAGG ATGAGAGATA CGACGAGGAG GAAGAGGCCG AAAGGGATCG GTTAAATATC 
AAGTCTCCGT TTTCTCTGAG TCACGTTTCT CGCGGGAAGA AGAAGCAGCA CGGAGAATAC 
AAGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179834.1
CDS	112..2220
Translation	MLRGRSLSVTSLGGLPRWEAGLPVENLLLFEVSWEVTNKVGGIYTVIQTKAKTTADEWGDNYFLIGPYFEHNMKTQVEQCEPVNDAVRRAMDTMNKHGCQVRFGRWLIEGSPYVVLFDIGYSAWNLDKWKGDLWEACSVGIPYHDQEANDMLIFGSLTAWFLKEVTDHADGRHVIVQFHEWQAGIGLILSRARKLPIATVFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQEFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTAHSVKEKFGKKLYDALLRGEIPDMNNILDRDDMTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNSRTDRVKLILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFCSPDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPEKFHMEPTSPPTTEGFKYPRPPSVPPSPSGSQGSSPQSSDAEDDDEDERYDEEEEAERDRLNIKSPFSLSHVSRGKKKQHGEYKN

Target ORF information:

RefSeq Version	NM_001192905.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen synthase 2 (GYS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192905.1

ORF Insert Sequence:

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGT TGATTTTGCA CCCAGAGTTC 
CTGTCCTCCA CCAGCCCCTT GCTACCCATG GATTATGAAG AGTTTGTCCG AGGTTGTCAT 
CTTGGAGTAT TTCCATCTTA CTATGAACCC TGGGGTTATA CTCCAGCTGA ATGCACTGTG 
ATGGGCATCC CCAGTGTGAC CACCAATCTC TCTGGCTTTG GCTGTTTCAT GCAGGAACAT 
GTGGCTGACC CCACTGCATA CGGAATTTAC ATCGTTGACA GGCGGTTCTG CTCACCCGAT 
GATTCTTGCA ATCAGCTGAC TCAGTTTCTC TATGGATTTT GCAAACAGTC ACGCCGCCAG 
AGGATTATTC AAAGGAATCG AACCGAGAGG CTCTCAGATC TTCTGGACTG GAGATACTTA 
GGCAGATATT ACCAGCATGC CAGGCACCTG ACATTAAGCA GAGCCTTTCC AGAAAAATTC 
CACATGGAAC CCACATCACC ACCAACGACA GAAGGGTTTA AGTATCCCAG GCCGCCCTCG 
GTACCACCTT CTCCGTCGGG ATCTCAGGGC TCCAGTCCTC AGAGCAGCGA TGCGGAGGAT 
GACGATGAGG ATGAGAGATA CGACGAGGAG GAAGAGGCCG AAAGGGATCG GTTAAATATC 
AAGTCTCCGT TTTCTCTGAG TCACGTTTCT CGCGGGAAGA AGAAGCAGCA CGGAGAATAC 
AAGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GYS2 ( XM_005206956.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207013.1 Bos taurus glycogen synthase 2 (GYS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19664
Clone ID Related Accession (Same CDS sequence)	XM_005206956.3
Accession Version	XM_005206956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen [starch] synthase, liver isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103934.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206956.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGA TTTTTTTTCT CTTTCAGTTG 
ATTTTGCACC CAGAGTTCCT GTCCTCCACC AGCCCCTTGC TACCCATGGA TTATGAAGAG 
TTTGTCCGAG GTTGTCATCT TGGAGTATTT CCATCTTACT ATGAACCCTG GGGTTATACT 
CCAGCTGAAT GCACTGTGAT GGGCATCCCC AGTGTGACCA CCAATCTCTC TGGCTTTGGC 
TGTTTCATGC AGGAACATGT GGCTGACCCC ACTGCATACG GAATTTACAT CGTTGACAGG 
CGGTTCTGCT CACCCGATGA TTCTTGCAAT CAGCTGACTC AGTTTCTCTA TGGATTTTGC 
AAACAGTCAC GCCGCCAGAG GATTATTCAA AGGAATCGAA CCGAGAGGCT CTCAGATCTT 
CTGGACTGGA GATACTTAGG CAGATATTAC CAGCATGCCA GGCACCTGAC ATTAAGCAGA 
GCCTTTCCAG AAAAATTCCA CATGGAACCC ACATCACCAC CAACGACAGA AGGGTTTAAG 
TATCCCAGGC CGCCCTCGGT ACCACCTTCT CCGTCGGGAT CTCAGGGCTC CAGTCCTCAG 
AGCAGCGATG CGGAGGATGA CGATGAGGAT GAGAGATACG ACGAGGAGGA AGAGGCCGAA 
AGGGATCGGT TAAATATCAA GTCTCCGTTT TCTCTGAGTC ACGTTTCTCG CGGGAAGAAG 
AAGCAGCACG GAGAATACAA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207013.1
CDS	239..2365
Translation	MLRGRSLSVTSLGGLPRWEAGLPVENLLLFEVSWEVTNKVGGIYTVIQTKAKTTADEWGDNYFLIGPYFEHNMKTQVEQCEPVNDAVRRAMDTMNKHGCQVRFGRWLIEGSPYVVLFDIGYSAWNLDKWKGDLWEACSVGIPYHDQEANDMLIFGSLTAWFLKEVTDHADGRHVIVQFHEWQAGIGLILSRARKLPIATVFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQEFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTAHSVKEKFGKKLYDALLRGEIPDMNNILDRDDMTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNSRTDRVKIFFLFQLILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFCSPDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPEKFHMEPTSPPTTEGFKYPRPPSVPPSPSGSQGSSPQSSDAEDDDEDERYDEEEEAERDRLNIKSPFSLSHVSRGKKKQHGEYKN

Target ORF information:

RefSeq Version	XM_005206956.3
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen synthase 2 (GYS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206956.3

ORF Insert Sequence:

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGA TTTTTTTTCT CTTTCAGTTG 
ATTTTGCACC CAGAGTTCCT GTCCTCCACC AGCCCCTTGC TACCCATGGA TTATGAAGAG 
TTTGTCCGAG GTTGTCATCT TGGAGTATTT CCATCTTACT ATGAACCCTG GGGTTATACT 
CCAGCTGAAT GCACTGTGAT GGGCATCCCC AGTGTGACCA CCAATCTCTC TGGCTTTGGC 
TGTTTCATGC AGGAACATGT GGCTGACCCC ACTGCATACG GAATTTACAT CGTTGACAGG 
CGGTTCTGCT CACCCGATGA TTCTTGCAAT CAGCTGACTC AGTTTCTCTA TGGATTTTGC 
AAACAGTCAC GCCGCCAGAG GATTATTCAA AGGAATCGAA CCGAGAGGCT CTCAGATCTT 
CTGGACTGGA GATACTTAGG CAGATATTAC CAGCATGCCA GGCACCTGAC ATTAAGCAGA 
GCCTTTCCAG AAAAATTCCA CATGGAACCC ACATCACCAC CAACGACAGA AGGGTTTAAG 
TATCCCAGGC CGCCCTCGGT ACCACCTTCT CCGTCGGGAT CTCAGGGCTC CAGTCCTCAG 
AGCAGCGATG CGGAGGATGA CGATGAGGAT GAGAGATACG ACGAGGAGGA AGAGGCCGAA 
AGGGATCGGT TAAATATCAA GTCTCCGTTT TCTCTGAGTC ACGTTTCTCG CGGGAAGAAG 
AAGCAGCACG GAGAATACAA GAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GYS2 ( XM_010805421.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803723.1 Bos taurus glycogen synthase 2 (GYS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa53654
Clone ID Related Accession (Same CDS sequence)	XM_010805421.2
Accession Version	XM_010805421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen [starch] synthase, liver isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103934.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805421.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGA TTTTTTTTCT CTTTCAGTTG 
ATTTTGCACC CAGAGTTCCT GTCCTCCACC AGCCCCTTGC TACCCATGGA TTATGAAGAG 
TTTGTCCGAG GTTGTCATCT TGGAGTATTT CCATCTTACT ATGAACCCTG GGGTTATACT 
CCAGGAATTT ACATCGTTGA CAGGCGGTTC TGCTCACCCG ATGATTCTTG CAATCAGCTG 
ACTCAGTTTC TCTATGGATT TTGCAAACAG TCACGCCGCC AGAGGATTAT TCAAAGGAAT 
CGAACCGAGA GGCTCTCAGA TCTTCTGGAC TGGAGATACT TAGGCAGATA TTACCAGCAT 
GCCAGGCACC TGACATTAAG CAGAGCCTTT CCAGAAAAAT TCCACATGGA ACCCACATCA 
CCACCAACGA CAGAAGGGTT TAAGTATCCC AGGCCGCCCT CGGTACCACC TTCTCCGTCG 
GGATCTCAGG GCTCCAGTCC TCAGAGCAGC GATGCGGAGG ATGACGATGA GGATGAGAGA 
TACGACGAGG AGGAAGAGGC CGAAAGGGAT CGGTTAAATA TCAAGTCTCC GTTTTCTCTG 
AGTCACGTTT CTCGCGGGAA GAAGAAGCAG CACGGAGAAT ACAAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803723.1
CDS	241..2271
Translation	MLRGRSLSVTSLGGLPRWEAGLPVENLLLFEVSWEVTNKVGGIYTVIQTKAKTTADEWGDNYFLIGPYFEHNMKTQVEQCEPVNDAVRRAMDTMNKHGCQVRFGRWLIEGSPYVVLFDIGYSAWNLDKWKGDLWEACSVGIPYHDQEANDMLIFGSLTAWFLKEVTDHADGRHVIVQFHEWQAGIGLILSRARKLPIATVFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQEFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTAHSVKEKFGKKLYDALLRGEIPDMNNILDRDDMTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNSRTDRVKIFFLFQLILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPGIYIVDRRFCSPDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPEKFHMEPTSPPTTEGFKYPRPPSVPPSPSGSQGSSPQSSDAEDDDEDERYDEEEEAERDRLNIKSPFSLSHVSRGKKKQHGEYKN

Target ORF information:

RefSeq Version	XM_010805421.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen synthase 2 (GYS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805421.2

ORF Insert Sequence:

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGA TTTTTTTTCT CTTTCAGTTG 
ATTTTGCACC CAGAGTTCCT GTCCTCCACC AGCCCCTTGC TACCCATGGA TTATGAAGAG 
TTTGTCCGAG GTTGTCATCT TGGAGTATTT CCATCTTACT ATGAACCCTG GGGTTATACT 
CCAGGAATTT ACATCGTTGA CAGGCGGTTC TGCTCACCCG ATGATTCTTG CAATCAGCTG 
ACTCAGTTTC TCTATGGATT TTGCAAACAG TCACGCCGCC AGAGGATTAT TCAAAGGAAT 
CGAACCGAGA GGCTCTCAGA TCTTCTGGAC TGGAGATACT TAGGCAGATA TTACCAGCAT 
GCCAGGCACC TGACATTAAG CAGAGCCTTT CCAGAAAAAT TCCACATGGA ACCCACATCA 
CCACCAACGA CAGAAGGGTT TAAGTATCCC AGGCCGCCCT CGGTACCACC TTCTCCGTCG 
GGATCTCAGG GCTCCAGTCC TCAGAGCAGC GATGCGGAGG ATGACGATGA GGATGAGAGA 
TACGACGAGG AGGAAGAGGC CGAAAGGGAT CGGTTAAATA TCAAGTCTCC GTTTTCTCTG 
AGTCACGTTT CTCGCGGGAA GAAGAAGCAG CACGGAGAAT ACAAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GYS2 ( NM_001192905.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179834.2 Bos taurus glycogen synthase 2 (GYS2), mRNA.

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CloneID	OBa239153
Clone ID Related Accession (Same CDS sequence)	NM_001192905.2
Accession Version	NM_001192905.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Bos taurus(cattle)
Product	glycogen [starch] synthase, liver
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1 and AW311782.1. On Jun 18, 2018 this sequence version replaced NM_001192905.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.40833.1, SRR5571257.51918.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145427, SAMN04019641 [ECO:0000348] ##Evidence-Data-END##

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGT TGATTTTGCA CCCAGAGTTC 
CTGTCCTCCA CCAGCCCCTT GCTACCCATG GATTATGAAG AGTTTGTCCG AGGTTGTCAT 
CTTGGAGTAT TTCCATCTTA CTATGAACCC TGGGGTTATA CTCCAGCTGA ATGCACTGTG 
ATGGGCATCC CCAGTGTGAC CACCAATCTC TCTGGCTTTG GCTGTTTCAT GCAGGAACAT 
GTGGCTGACC CCACTGCATA CGGAATTTAC ATCGTTGACA GGCGGTTCTG CTCACCCGAT 
GATTCTTGCA ATCAGCTGAC TCAGTTTCTC TATGGATTTT GCAAACAGTC ACGCCGCCAG 
AGGATTATTC AAAGGAATCG AACCGAGAGG CTCTCAGATC TTCTGGACTG GAGATACTTA 
GGCAGATATT ACCAGCATGC CAGGCACCTG ACATTAAGCA GAGCCTTTCC AGAAAAATTC 
CACATGGAAC CCACATCACC ACCAACGACA GAAGGGTTTA AGTATCCCAG GCCGCCCTCG 
GTACCACCTT CTCCGTCGGG ATCTCAGGGC TCCAGTCCTC AGAGCAGCGA TGCGGAGGAT 
GACGATGAGG ATGAGAGATA CGACGAGGAG GAAGAGGCCG AAAGGGATCG GTTAAATATC 
AAGTCTCCGT TTTCTCTGAG TCACGTTCCT CGCGGCAAGA AGAAGCAGCA CGGAGAATAC 
AAGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179834.2
CDS	112..2220
Translation	MLRGRSLSVTSLGGLPRWEAGLPVENLLLFEVSWEVTNKVGGIYTVIQTKAKTTADEWGDNYFLIGPYFEHNMKTQVEQCEPVNDAVRRAMDTMNKHGCQVRFGRWLIEGSPYVVLFDIGYSAWNLDKWKGDLWEACSVGIPYHDQEANDMLIFGSLTAWFLKEVTDHADGRHVIVQFHEWQAGIGLILSRARKLPIATVFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQEFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTAHSVKEKFGKKLYDALLRGEIPDMNNILDRDDMTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNSRTDRVKLILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFCSPDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPEKFHMEPTSPPTTEGFKYPRPPSVPPSPSGSQGSSPQSSDAEDDDEDERYDEEEEAERDRLNIKSPFSLSHVPRGKKKQHGEYKN

Target ORF information:

RefSeq Version	NM_001192905.2
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen synthase 2 (GYS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192905.2

ORF Insert Sequence:

ATGCTTCGAG GTCGATCGCT ATCTGTGACA TCCCTAGGTG GGCTGCCTCG GTGGGAAGCC 
GGACTTCCTG TGGAGAATTT ACTGCTCTTT GAAGTCTCTT GGGAAGTGAC CAATAAAGTC 
GGTGGCATCT ATACTGTGAT TCAGACAAAG GCCAAAACAA CAGCAGATGA ATGGGGAGAC 
AACTATTTTC TGATTGGTCC ATATTTTGAG CATAATATGA AGACTCAGGT GGAACAGTGT 
GAACCTGTGA ATGATGCTGT TAGAAGAGCC ATGGACACGA TGAACAAACA CGGCTGCCAG 
GTGCGCTTTG GAAGATGGCT GATAGAAGGG AGTCCTTACG TGGTTCTCTT TGACATTGGC 
TATTCGGCTT GGAACCTGGA CAAGTGGAAA GGAGACCTCT GGGAAGCATG CAGCGTTGGC 
ATTCCTTATC ACGACCAGGA AGCCAATGAT ATGCTGATCT TTGGGTCATT AACTGCCTGG 
TTCTTGAAAG AGGTGACGGA CCATGCTGAT GGGAGACATG TCATTGTCCA GTTCCATGAA 
TGGCAAGCTG GGATTGGACT GATCCTTTCT CGAGCTCGGA AACTGCCCAT TGCTACAGTC 
TTTACCACCC ACGCCACACT GCTTGGGCGG TATCTTTGTG CAGCTAACAT CGATTTCTAC 
AACCACCTTG ATAAGTTTAA CATAGACAAA GAGGCTGGGG AAAGGCAGAT TTATCACCGG 
TATTGCATGG AGCGCGCTTC CGTCCATTGT GCTCATGTTT TCACCACAGT TTCTGAAATT 
ACCGCAATAG AGGCAGAACA CATGCTGAAG AGAAAACCTG ATGTAGTTAC TCCAAATGGC 
TTGAACGTTA AGAAATTTTC AGCTGTACAT GAGTTTCAAA ATCTCCATGC TATGTACAAG 
GCCAGAATCC AAGAGTTTGT TCGAGGTCAT TTCTATGGTC ATCTGGACTT TGATCTTGAA 
AAAACGTTAT TCCTCTTCAT TGCTGGGAGG TATGAGTTTT CAAACAAAGG AGCTGACATC 
TTCTTAGAAT CCTTATCCAG GTTAAACTTC CTGCTGAGGA TGCATAAAAG TGATGTCACC 
GTGGTGGTGT TTTTCATTAT GCCTGCCAAG ACAAACAATT TCAATGTGGA AACCCTGAAA 
GGCCAGGCAG TGCGCAAACA GCTATGGGAC ACTGCACATT CTGTGAAGGA AAAGTTTGGA 
AAGAAACTGT ATGATGCATT ATTAAGAGGA GAAATTCCTG ACATGAACAA TATTTTGGAC 
CGAGATGATA TGACAATTAT GAAAAGAGCT ATCTTTTCAA CTCAGCGGCA GTCTTTGCCC 
CCAGTGACTA CTCACAACAT GATTGATGAT TCCACCGATC CCATCCTCAG CACCATTAGA 
CGTATCGGGC TCTTCAATAG CCGCACAGAC AGAGTCAAGT TGATTTTGCA CCCAGAGTTC 
CTGTCCTCCA CCAGCCCCTT GCTACCCATG GATTATGAAG AGTTTGTCCG AGGTTGTCAT 
CTTGGAGTAT TTCCATCTTA CTATGAACCC TGGGGTTATA CTCCAGCTGA ATGCACTGTG 
ATGGGCATCC CCAGTGTGAC CACCAATCTC TCTGGCTTTG GCTGTTTCAT GCAGGAACAT 
GTGGCTGACC CCACTGCATA CGGAATTTAC ATCGTTGACA GGCGGTTCTG CTCACCCGAT 
GATTCTTGCA ATCAGCTGAC TCAGTTTCTC TATGGATTTT GCAAACAGTC ACGCCGCCAG 
AGGATTATTC AAAGGAATCG AACCGAGAGG CTCTCAGATC TTCTGGACTG GAGATACTTA 
GGCAGATATT ACCAGCATGC CAGGCACCTG ACATTAAGCA GAGCCTTTCC AGAAAAATTC 
CACATGGAAC CCACATCACC ACCAACGACA GAAGGGTTTA AGTATCCCAG GCCGCCCTCG 
GTACCACCTT CTCCGTCGGG ATCTCAGGGC TCCAGTCCTC AGAGCAGCGA TGCGGAGGAT 
GACGATGAGG ATGAGAGATA CGACGAGGAG GAAGAGGCCG AAAGGGATCG GTTAAATATC 
AAGTCTCCGT TTTCTCTGAG TCACGTTCCT CGCGGCAAGA AGAAGCAGCA CGGAGAATAC 
AAGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GYS2 ( XM_015471237.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326723.1 Bos taurus glycogen synthase 2 (GYS2), transcript variant X3, mRNA.

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CloneID	OBa90796
Clone ID Related Accession (Same CDS sequence)	XM_015471237.1
Accession Version	XM_015471237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	glycogen [starch] synthase, liver isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103934.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACTC AGGTGGAACA GTGTGAACCT GTGAATGATG CTGTTAGAAG AGCCATGGAC 
ACGATGAACA AACACGGCTG CCAGGTGCGC TTTGGAAGAT GGCTGATAGA AGGGAGTCCT 
TACGTGGTTC TCTTTGACAT TGGCTATTCG GCTTGGAACC TGGACAAGTG GAAAGGAGAC 
CTCTGGGAAG CATGCAGCGT TGGCATTCCT TATCACGACC AGGAAGCCAA TGATATGCTG 
ATCTTTGGGT CATTAACTGC CTGGTTCTTG AAAGAGGTGA CGGACCATGC TGATGGGAGA 
CATGTCATTG TCCAGTTCCA TGAATGGCAA GCTGGGATTG GACTGATCCT TTCTCGAGCT 
CGGAAACTGC CCATTGCTAC AGTCTTTACC ACCCACGCCA CACTGCTTGG GCGGTATCTT 
TGTGCAGCTA ACATCGATTT CTACAACCAC CTTGATAAGT TTAACATAGA CAAAGAGGCT 
GGGGAAAGGC AGATTTATCA CCGGTATTGC ATGGAGCGCG CTTCCGTCCA TTGTGCTCAT 
GTTTTCACCA CAGTTTCTGA AATTACCGCA ATAGAGGCAG AACACATGCT GAAGAGAAAA 
CCTGATGTAG TTACTCCAAA TGGCTTGAAC GTTAAGAAAT TTTCAGCTGT ACATGAGTTT 
CAAAATCTCC ATGCTATGTA CAAGGCCAGA ATCCAAGAGT TTGTTCGAGG TCATTTCTAT 
GGTCATCTGG ACTTTGATCT TGAAAAAACG TTATTCCTCT TCATTGCTGG GAGGTATGAG 
TTTTCAAACA AAGGAGCTGA CATCTTCTTA GAATCCTTAT CCAGGTTAAA CTTCCTGCTG 
AGGATGCATA AAAGTGATGT CACCGTGGTG GTGTTTTTCA TTATGCCTGC CAAGACAAAC 
AATTTCAATG TGGAAACCCT GAAAGGCCAG GCAGTGCGCA AACAGCTATG GGACACTGCA 
CATTCTGTGA AGGAAAAGTT TGGAAAGAAA CTGTATGATG CATTATTAAG AGGAGAAATT 
CCTGACATGA ACAATATTTT GGACCGAGAT GATATGACAA TTATGAAAAG AGCTATCTTT 
TCAACTCAGC GGCAGTCTTT GCCCCCAGTG ACTACTCACA ACATGATTGA TGATTCCACC 
GATCCCATCC TCAGCACCAT TAGACGTATC GGGCTCTTCA ATAGCCGCAC AGACAGAGTC 
AAGATTTTTT TTCTCTTTCA GTTGATTTTG CACCCAGAGT TCCTGTCCTC CACCAGCCCC 
TTGCTACCCA TGGATTATGA AGAGTTTGTC CGAGGTTGTC ATCTTGGAGT ATTTCCATCT 
TACTATGAAC CCTGGGGTTA TACTCCAGCT GAATGCACTG TGATGGGCAT CCCCAGTGTG 
ACCACCAATC TCTCTGGCTT TGGCTGTTTC ATGCAGGAAC ATGTGGCTGA CCCCACTGCA 
TACGGAATTT ACATCGTTGA CAGGCGGTTC TGCTCACCCG ATGATTCTTG CAATCAGCTG 
ACTCAGTTTC TCTATGGATT TTGCAAACAG TCACGCCGCC AGAGGATTAT TCAAAGGAAT 
CGAACCGAGA GGCTCTCAGA TCTTCTGGAC TGGAGATACT TAGGCAGATA TTACCAGCAT 
GCCAGGCACC TGACATTAAG CAGAGCCTTT CCAGAAAAAT TCCACATGGA ACCCACATCA 
CCACCAACGA CAGAAGGGTT TAAGTATCCC AGGCCGCCCT CGGTACCACC TTCTCCGTCG 
GGATCTCAGG GCTCCAGTCC TCAGAGCAGC GATGCGGAGG ATGACGATGA GGATGAGAGA 
TACGACGAGG AGGAAGAGGC CGAAAGGGAT CGGTTAAATA TCAAGTCTCC GTTTTCTCTG 
AGTCACGTTT CTCGCGGGAA GAAGAAGCAG CACGGAGAAT ACAAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326723.1
CDS	815..2725
Translation	MKTQVEQCEPVNDAVRRAMDTMNKHGCQVRFGRWLIEGSPYVVLFDIGYSAWNLDKWKGDLWEACSVGIPYHDQEANDMLIFGSLTAWFLKEVTDHADGRHVIVQFHEWQAGIGLILSRARKLPIATVFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQEFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDVTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTAHSVKEKFGKKLYDALLRGEIPDMNNILDRDDMTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNSRTDRVKIFFLFQLILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFCSPDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPEKFHMEPTSPPTTEGFKYPRPPSVPPSPSGSQGSSPQSSDAEDDDEDERYDEEEEAERDRLNIKSPFSLSHVSRGKKKQHGEYKN

Target ORF information:

RefSeq Version	XM_015471237.1
Organism	Bos taurus(cattle)
Definition	Bos taurus glycogen synthase 2 (GYS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471237.1

ORF Insert Sequence:

ATGAAGACTC AGGTGGAACA GTGTGAACCT GTGAATGATG CTGTTAGAAG AGCCATGGAC 
ACGATGAACA AACACGGCTG CCAGGTGCGC TTTGGAAGAT GGCTGATAGA AGGGAGTCCT 
TACGTGGTTC TCTTTGACAT TGGCTATTCG GCTTGGAACC TGGACAAGTG GAAAGGAGAC 
CTCTGGGAAG CATGCAGCGT TGGCATTCCT TATCACGACC AGGAAGCCAA TGATATGCTG 
ATCTTTGGGT CATTAACTGC CTGGTTCTTG AAAGAGGTGA CGGACCATGC TGATGGGAGA 
CATGTCATTG TCCAGTTCCA TGAATGGCAA GCTGGGATTG GACTGATCCT TTCTCGAGCT 
CGGAAACTGC CCATTGCTAC AGTCTTTACC ACCCACGCCA CACTGCTTGG GCGGTATCTT 
TGTGCAGCTA ACATCGATTT CTACAACCAC CTTGATAAGT TTAACATAGA CAAAGAGGCT 
GGGGAAAGGC AGATTTATCA CCGGTATTGC ATGGAGCGCG CTTCCGTCCA TTGTGCTCAT 
GTTTTCACCA CAGTTTCTGA AATTACCGCA ATAGAGGCAG AACACATGCT GAAGAGAAAA 
CCTGATGTAG TTACTCCAAA TGGCTTGAAC GTTAAGAAAT TTTCAGCTGT ACATGAGTTT 
CAAAATCTCC ATGCTATGTA CAAGGCCAGA ATCCAAGAGT TTGTTCGAGG TCATTTCTAT 
GGTCATCTGG ACTTTGATCT TGAAAAAACG TTATTCCTCT TCATTGCTGG GAGGTATGAG 
TTTTCAAACA AAGGAGCTGA CATCTTCTTA GAATCCTTAT CCAGGTTAAA CTTCCTGCTG 
AGGATGCATA AAAGTGATGT CACCGTGGTG GTGTTTTTCA TTATGCCTGC CAAGACAAAC 
AATTTCAATG TGGAAACCCT GAAAGGCCAG GCAGTGCGCA AACAGCTATG GGACACTGCA 
CATTCTGTGA AGGAAAAGTT TGGAAAGAAA CTGTATGATG CATTATTAAG AGGAGAAATT 
CCTGACATGA ACAATATTTT GGACCGAGAT GATATGACAA TTATGAAAAG AGCTATCTTT 
TCAACTCAGC GGCAGTCTTT GCCCCCAGTG ACTACTCACA ACATGATTGA TGATTCCACC 
GATCCCATCC TCAGCACCAT TAGACGTATC GGGCTCTTCA ATAGCCGCAC AGACAGAGTC 
AAGATTTTTT TTCTCTTTCA GTTGATTTTG CACCCAGAGT TCCTGTCCTC CACCAGCCCC 
TTGCTACCCA TGGATTATGA AGAGTTTGTC CGAGGTTGTC ATCTTGGAGT ATTTCCATCT 
TACTATGAAC CCTGGGGTTA TACTCCAGCT GAATGCACTG TGATGGGCAT CCCCAGTGTG 
ACCACCAATC TCTCTGGCTT TGGCTGTTTC ATGCAGGAAC ATGTGGCTGA CCCCACTGCA 
TACGGAATTT ACATCGTTGA CAGGCGGTTC TGCTCACCCG ATGATTCTTG CAATCAGCTG 
ACTCAGTTTC TCTATGGATT TTGCAAACAG TCACGCCGCC AGAGGATTAT TCAAAGGAAT 
CGAACCGAGA GGCTCTCAGA TCTTCTGGAC TGGAGATACT TAGGCAGATA TTACCAGCAT 
GCCAGGCACC TGACATTAAG CAGAGCCTTT CCAGAAAAAT TCCACATGGA ACCCACATCA 
CCACCAACGA CAGAAGGGTT TAAGTATCCC AGGCCGCCCT CGGTACCACC TTCTCCGTCG 
GGATCTCAGG GCTCCAGTCC TCAGAGCAGC GATGCGGAGG ATGACGATGA GGATGAGAGA 
TACGACGAGG AGGAAGAGGC CGAAAGGGAT CGGTTAAATA TCAAGTCTCC GTTTTCTCTG 
AGTCACGTTT CTCGCGGGAA GAAGAAGCAG CACGGAGAAT ACAAGAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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GYS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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