PHEX cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PHEX
Entrez Gene ID	536029
Full Name	phosphate regulating endopeptidase homolog X-linked
General protein information	Preferred Names phosphate regulating endopeptidase homolog X-linked Names phosphate-regulating neutral endopeptidase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001192871.2	NP_001179800.2	phosphate-regulating neutral endopeptidase
XM_024988135.1	XP_024843903.1	phosphate-regulating neutral endopeptidase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHEX	XP_003317428.1
Canis lupus familiaris (dog)	PHEX	XP_005641253.1
Gallus gallus (chicken)	PHEX	NP_001186206.1
Xenopus tropicalis (tropical clawed frog)	phex	XP_004911718.1
Homo sapiens (human)	PHEX	NP_000435.3
Mus musculus (house mouse)	Phex	NP_035207.1
Bos taurus (cattle)	PHEX	NP_001179800.2
Danio rerio (zebrafish)	phex	NP_001082818.1
	PHEX	XP_001087261.1
Rattus norvegicus (Norway rat)	Phex	NP_037136.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs.
Takasuga A, Hirotsune S, Itoh R, Jitohzono A, Suzuki H, Aso H, Sugimoto Y
Nucleic acids research29(22)E108(2001 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PHEX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHEX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01809	NM_001192871.2 Latest version!	Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01809	XM_024988135.1 Latest version!	Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PHEX ( NM_001192871.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179800.2 Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), mRNA.

Price & Availability↑

CloneID	OBa01809
Clone ID Related Accession (Same CDS sequence)	NM_001192871.2 , XM_024988135.1
Accession Version	NM_001192871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-24
Organism	Bos taurus(cattle)
Product	phosphate-regulating neutral endopeptidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AV594764.1 and NKLS02000030.1. On Apr 26, 2011 this sequence version replaced NM_001192871.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The genomic coordinates used for the transcript record were based on transcript alignments, including human NM_000444.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGAGGCAG AAACAGGGAG CAGCATGGAG ATCGGAAAGA CAGCCAACAG AGGCACTCGA 
ATCGCCCTGG CGGTGTTCGT TGGTGGCACC CTGGTGCTGG GCATAGTCCT CTTTCTAGTG 
AGTCAAGGTT TCTTAAGTCT CCATGCTAAA CCAGAGTACT GCCTGAAGCC AGAATGCATC 
GAAGCTGCTG CTTCCATCTT AAGTAAAGTA AATCTGTCTG TGGACCCTTG TGATAATTTC 
TTCCGGTTTG CTTGTGATGG CTGGATACAC AGTAATCCAA TTCCTGAAGA TATGCCAAGC 
TATGGGATTT ATCCTTGGCT CAGACGTAAT GTCGACCTCA AGTTGAAGGC ACTTTTGGAG 
AAATCAATCA GTAGAAGACG GGACACTGAA GCAATACAGA AAGCCAAAAT CCTTTATTCA 
TCTTGCATGA ATGAGAAAGC CATCGAAAAA GCGGATGCCA AGCCACTACT ACATATCCTG 
CGGCACTCAC CTTTCCGCTG GCCTGTGCTC GAATCCAATA TTGGTCCTGA AGGGGTCTGG 
TCAGAGAGAA AGTTCAGCCT TCTGCAGACC CTTGCAACGT TTCGTGGCCA ATACAGCAAT 
TCTGTGTTCA TCCGTTTATA TGTGTCCTCT GATGACAAGA TGTCCAACGA ACACATCTTG 
AAGCTGGACC AAGCGGCGCT CTCCCTAGCC GTGAGGGAAG ACTACCTTGA TAACAGCACG 
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAATTCATGG TGGATACTGC TGTGCTTTTG 
GGAGCTAATA GCTCCCGAGC AGAGCATGAC ATGAAGTCGG TGCTTAGGTT GGAAATAAAG 
ATAGCTGAGA TAATGATTCC GCATGAAAAC CGAACCAGTG AAGCCATGTA CAACAAAATG 
AACATCTCCC AGCTGAGTGC TATGATTCCC CAGTTTGACT GGCTGAGCTA CATCAAGAAG 
GTCATCGATG TCAGACTCTA CCCTGAACTG AAAGACATTG GCCCCTCAGA GAACGTGGTG 
GTCCGAGTCC CTCAATACTT TAAGGATTTG TTTAGGATAT TAGGCTCTGA GAGGAAGAAG 
ACCATTGCCA ACTATTTGGT GTGGAGGATG GTTTATTCCA GAATTCCAAA CTTAAGCAGG 
CGCTTTCAGT ATAGGTGGCT GGAGTTCTCA CGGGTAATCC AGGGGACCAC AACTTTGTTG 
CCTCAGTGGG ACAAATGTGT GAACTTCATT GAAAGTACTC TCCCTTATGT CGTTGGAAAA 
ATGTTTGTGA ATGTACACTT CCAGGAAGAC AAGAAGGAAA TGATGGAGGA ATTGATTGAG 
GGTGTTCGAT GGGCCTTTAT TGACATGCTG GAGAAAGAAA ATGAGTGGAT GGATGCAGGG 
ACAAAAAGGA AAGCCAAAGA AAAGGCAAGA GCTGTTTTGG CAAAAGTTGG CTATCCTGAG 
TTTATAATGA ATGATACTCA TATTAATGAA GACCTCAAGG CAATCAAGTT TTCAGAATCT 
GACTACTTTG GCAACGTCCT GCAAACCCGC AAGTATTTAG CACAGACTGA TTTCTTCTGG 
CTGAGAAAAG CTGTTCCCAA AACAGAGTGG TTTACAAATC CAACGACTGT CAATGCCTTC 
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AACTCCAGAA GCCTTTCTTT 
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTTGGACAT 
GAATTTACAC ATGGATTTGA TAATAATGGT CGAAAATATG ATAAAAATGG AAACCTGGAT 
CCTTGGTGGT CTATGGATTC AGAAGAAAAG TTTAAGGAAA AAACAAAGTG CATGATTAAC 
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TGAAGGGGAA GAGGACACTG 
GGAGAAAATA TTGCTGATAA TGGAGGGCTG CGACAAGCTT TTAGGGCTTA CAGGAAATGG 
ATAAATGACA AGAGGCAGGG AGTTGAGGAG CCTCTCCTAC CAGGCATTGA ATTCACCAAC 
AACCAGCTTT TCTTCATGAG TTATGCTCAC GTGAGGTGCA ATTCCTACAG ACCAGAAGCT 
GCTCGAGAAC AAATCCAAGC TGGTGCTCAC AGCCCCCCAC AGTTTAGGGT CAATGGTGCA 
GTTAGCAACT TTGAAGAATT CCAGAAGGCT TTTAATTGTC CATCCAATTC CACAATGAAC 
AGAGGCGCAG AATCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179800.2
CDS	352..2601
Translation	MEAETGSSMEIGKTANRGTRIALAVFVGGTLVLGIVLFLVSQGFLSLHAKPEYCLKPECIEAAASILSKVNLSVDPCDNFFRFACDGWIHSNPIPEDMPSYGIYPWLRRNVDLKLKALLEKSISRRRDTEAIQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSSDDKMSNEHILKLDQAALSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISQLSAMIPQFDWLSYIKKVIDVRLYPELKDIGPSENVVVRVPQYFKDLFRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSRVIQGTTTLLPQWDKCVNFIESTLPYVVGKMFVNVHFQEDKKEMMEELIEGVRWAFIDMLEKENEWMDAGTKRKAKEKARAVLAKVGYPEFIMNDTHINEDLKAIKFSESDYFGNVLQTRKYLAQTDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFFWGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSMDSEEKFKEKTKCMINQYSNYYWKKAGLNVKGKRTLGENIADNGGLRQAFRAYRKWINDKRQGVEEPLLPGIEFTNNQLFFMSYAHVRCNSYRPEAAREQIQAGAHSPPQFRVNGAVSNFEEFQKAFNCPSNSTMNRGAESCRLW

Target ORF information:

RefSeq Version	NM_001192871.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192871.2

ORF Insert Sequence:

ATGGAGGCAG AAACAGGGAG CAGCATGGAG ATCGGAAAGA CAGCCAACAG AGGCACTCGA 
ATCGCCCTGG CGGTGTTCGT TGGTGGCACC CTGGTGCTGG GCATAGTCCT CTTTCTAGTG 
AGTCAAGGTT TCTTAAGTCT CCATGCTAAA CCAGAGTACT GCCTGAAGCC AGAATGCATC 
GAAGCTGCTG CTTCCATCTT AAGTAAAGTA AATCTGTCTG TGGACCCTTG TGATAATTTC 
TTCCGGTTTG CTTGTGATGG CTGGATACAC AGTAATCCAA TTCCTGAAGA TATGCCAAGC 
TATGGGATTT ATCCTTGGCT CAGACGTAAT GTCGACCTCA AGTTGAAGGC ACTTTTGGAG 
AAATCAATCA GTAGAAGACG GGACACTGAA GCAATACAGA AAGCCAAAAT CCTTTATTCA 
TCTTGCATGA ATGAGAAAGC CATCGAAAAA GCGGATGCCA AGCCACTACT ACATATCCTG 
CGGCACTCAC CTTTCCGCTG GCCTGTGCTC GAATCCAATA TTGGTCCTGA AGGGGTCTGG 
TCAGAGAGAA AGTTCAGCCT TCTGCAGACC CTTGCAACGT TTCGTGGCCA ATACAGCAAT 
TCTGTGTTCA TCCGTTTATA TGTGTCCTCT GATGACAAGA TGTCCAACGA ACACATCTTG 
AAGCTGGACC AAGCGGCGCT CTCCCTAGCC GTGAGGGAAG ACTACCTTGA TAACAGCACG 
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAATTCATGG TGGATACTGC TGTGCTTTTG 
GGAGCTAATA GCTCCCGAGC AGAGCATGAC ATGAAGTCGG TGCTTAGGTT GGAAATAAAG 
ATAGCTGAGA TAATGATTCC GCATGAAAAC CGAACCAGTG AAGCCATGTA CAACAAAATG 
AACATCTCCC AGCTGAGTGC TATGATTCCC CAGTTTGACT GGCTGAGCTA CATCAAGAAG 
GTCATCGATG TCAGACTCTA CCCTGAACTG AAAGACATTG GCCCCTCAGA GAACGTGGTG 
GTCCGAGTCC CTCAATACTT TAAGGATTTG TTTAGGATAT TAGGCTCTGA GAGGAAGAAG 
ACCATTGCCA ACTATTTGGT GTGGAGGATG GTTTATTCCA GAATTCCAAA CTTAAGCAGG 
CGCTTTCAGT ATAGGTGGCT GGAGTTCTCA CGGGTAATCC AGGGGACCAC AACTTTGTTG 
CCTCAGTGGG ACAAATGTGT GAACTTCATT GAAAGTACTC TCCCTTATGT CGTTGGAAAA 
ATGTTTGTGA ATGTACACTT CCAGGAAGAC AAGAAGGAAA TGATGGAGGA ATTGATTGAG 
GGTGTTCGAT GGGCCTTTAT TGACATGCTG GAGAAAGAAA ATGAGTGGAT GGATGCAGGG 
ACAAAAAGGA AAGCCAAAGA AAAGGCAAGA GCTGTTTTGG CAAAAGTTGG CTATCCTGAG 
TTTATAATGA ATGATACTCA TATTAATGAA GACCTCAAGG CAATCAAGTT TTCAGAATCT 
GACTACTTTG GCAACGTCCT GCAAACCCGC AAGTATTTAG CACAGACTGA TTTCTTCTGG 
CTGAGAAAAG CTGTTCCCAA AACAGAGTGG TTTACAAATC CAACGACTGT CAATGCCTTC 
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AACTCCAGAA GCCTTTCTTT 
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTTGGACAT 
GAATTTACAC ATGGATTTGA TAATAATGGT CGAAAATATG ATAAAAATGG AAACCTGGAT 
CCTTGGTGGT CTATGGATTC AGAAGAAAAG TTTAAGGAAA AAACAAAGTG CATGATTAAC 
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TGAAGGGGAA GAGGACACTG 
GGAGAAAATA TTGCTGATAA TGGAGGGCTG CGACAAGCTT TTAGGGCTTA CAGGAAATGG 
ATAAATGACA AGAGGCAGGG AGTTGAGGAG CCTCTCCTAC CAGGCATTGA ATTCACCAAC 
AACCAGCTTT TCTTCATGAG TTATGCTCAC GTGAGGTGCA ATTCCTACAG ACCAGAAGCT 
GCTCGAGAAC AAATCCAAGC TGGTGCTCAC AGCCCCCCAC AGTTTAGGGT CAATGGTGCA 
GTTAGCAACT TTGAAGAATT CCAGAAGGCT TTTAATTGTC CATCCAATTC CACAATGAAC 
AGAGGCGCAG AATCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHEX ( XM_024988135.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843903.1 Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa01809
Clone ID Related Accession (Same CDS sequence)	NM_001192871.2 , XM_024988135.1
Accession Version	XM_024988135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphate-regulating neutral endopeptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAACAGGGAG CAGCATGGAG ATCGGAAAGA CAGCCAACAG AGGCACTCGA 
ATCGCCCTGG CGGTGTTCGT TGGTGGCACC CTGGTGCTGG GCATAGTCCT CTTTCTAGTG 
AGTCAAGGTT TCTTAAGTCT CCATGCTAAA CCAGAGTACT GCCTGAAGCC AGAATGCATC 
GAAGCTGCTG CTTCCATCTT AAGTAAAGTA AATCTGTCTG TGGACCCTTG TGATAATTTC 
TTCCGGTTTG CTTGTGATGG CTGGATACAC AGTAATCCAA TTCCTGAAGA TATGCCAAGC 
TATGGGATTT ATCCTTGGCT CAGACGTAAT GTCGACCTCA AGTTGAAGGC ACTTTTGGAG 
AAATCAATCA GTAGAAGACG GGACACTGAA GCAATACAGA AAGCCAAAAT CCTTTATTCA 
TCTTGCATGA ATGAGAAAGC CATCGAAAAA GCGGATGCCA AGCCACTACT ACATATCCTG 
CGGCACTCAC CTTTCCGCTG GCCTGTGCTC GAATCCAATA TTGGTCCTGA AGGGGTCTGG 
TCAGAGAGAA AGTTCAGCCT TCTGCAGACC CTTGCAACGT TTCGTGGCCA ATACAGCAAT 
TCTGTGTTCA TCCGTTTATA TGTGTCCTCT GATGACAAGA TGTCCAACGA ACACATCTTG 
AAGCTGGACC AAGCGGCGCT CTCCCTAGCC GTGAGGGAAG ACTACCTTGA TAACAGCACG 
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAATTCATGG TGGATACTGC TGTGCTTTTG 
GGAGCTAATA GCTCCCGAGC AGAGCATGAC ATGAAGTCGG TGCTTAGGTT GGAAATAAAG 
ATAGCTGAGA TAATGATTCC GCATGAAAAC CGAACCAGTG AAGCCATGTA CAACAAAATG 
AACATCTCCC AGCTGAGTGC TATGATTCCC CAGTTTGACT GGCTGAGCTA CATCAAGAAG 
GTCATCGATG TCAGACTCTA CCCTGAACTG AAAGACATTG GCCCCTCAGA GAACGTGGTG 
GTCCGAGTCC CTCAATACTT TAAGGATTTG TTTAGGATAT TAGGCTCTGA GAGGAAGAAG 
ACCATTGCCA ACTATTTGGT GTGGAGGATG GTTTATTCCA GAATTCCAAA CTTAAGCAGG 
CGCTTTCAGT ATAGGTGGCT GGAGTTCTCA CGGGTAATCC AGGGGACCAC AACTTTGTTG 
CCTCAGTGGG ACAAATGTGT GAACTTCATT GAAAGTACTC TCCCTTATGT CGTTGGAAAA 
ATGTTTGTGA ATGTACACTT CCAGGAAGAC AAGAAGGAAA TGATGGAGGA ATTGATTGAG 
GGTGTTCGAT GGGCCTTTAT TGACATGCTG GAGAAAGAAA ATGAGTGGAT GGATGCAGGG 
ACAAAAAGGA AAGCCAAAGA AAAGGCAAGA GCTGTTTTGG CAAAAGTTGG CTATCCTGAG 
TTTATAATGA ATGATACTCA TATTAATGAA GACCTCAAGG CAATCAAGTT TTCAGAATCT 
GACTACTTTG GCAACGTCCT GCAAACCCGC AAGTATTTAG CACAGACTGA TTTCTTCTGG 
CTGAGAAAAG CTGTTCCCAA AACAGAGTGG TTTACAAATC CAACGACTGT CAATGCCTTC 
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AACTCCAGAA GCCTTTCTTT 
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTTGGACAT 
GAATTTACAC ATGGATTTGA TAATAATGGT CGAAAATATG ATAAAAATGG AAACCTGGAT 
CCTTGGTGGT CTATGGATTC AGAAGAAAAG TTTAAGGAAA AAACAAAGTG CATGATTAAC 
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TGAAGGGGAA GAGGACACTG 
GGAGAAAATA TTGCTGATAA TGGAGGGCTG CGACAAGCTT TTAGGGCTTA CAGGAAATGG 
ATAAATGACA AGAGGCAGGG AGTTGAGGAG CCTCTCCTAC CAGGCATTGA ATTCACCAAC 
AACCAGCTTT TCTTCATGAG TTATGCTCAC GTGAGGTGCA ATTCCTACAG ACCAGAAGCT 
GCTCGAGAAC AAATCCAAGC TGGTGCTCAC AGCCCCCCAC AGTTTAGGGT CAATGGTGCA 
GTTAGCAACT TTGAAGAATT CCAGAAGGCT TTTAATTGTC CATCCAATTC CACAATGAAC 
AGAGGCGCAG AATCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843903.1
CDS	531..2780
Translation	MEAETGSSMEIGKTANRGTRIALAVFVGGTLVLGIVLFLVSQGFLSLHAKPEYCLKPECIEAAASILSKVNLSVDPCDNFFRFACDGWIHSNPIPEDMPSYGIYPWLRRNVDLKLKALLEKSISRRRDTEAIQKAKILYSSCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSSDDKMSNEHILKLDQAALSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIKIAEIMIPHENRTSEAMYNKMNISQLSAMIPQFDWLSYIKKVIDVRLYPELKDIGPSENVVVRVPQYFKDLFRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSRVIQGTTTLLPQWDKCVNFIESTLPYVVGKMFVNVHFQEDKKEMMEELIEGVRWAFIDMLEKENEWMDAGTKRKAKEKARAVLAKVGYPEFIMNDTHINEDLKAIKFSESDYFGNVLQTRKYLAQTDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFFWGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSMDSEEKFKEKTKCMINQYSNYYWKKAGLNVKGKRTLGENIADNGGLRQAFRAYRKWINDKRQGVEEPLLPGIEFTNNQLFFMSYAHVRCNSYRPEAAREQIQAGAHSPPQFRVNGAVSNFEEFQKAFNCPSNSTMNRGAESCRLW

Target ORF information:

RefSeq Version	XM_024988135.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphate regulating endopeptidase homolog X-linked (PHEX), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024988135.1

ORF Insert Sequence:

ATGGAGGCAG AAACAGGGAG CAGCATGGAG ATCGGAAAGA CAGCCAACAG AGGCACTCGA 
ATCGCCCTGG CGGTGTTCGT TGGTGGCACC CTGGTGCTGG GCATAGTCCT CTTTCTAGTG 
AGTCAAGGTT TCTTAAGTCT CCATGCTAAA CCAGAGTACT GCCTGAAGCC AGAATGCATC 
GAAGCTGCTG CTTCCATCTT AAGTAAAGTA AATCTGTCTG TGGACCCTTG TGATAATTTC 
TTCCGGTTTG CTTGTGATGG CTGGATACAC AGTAATCCAA TTCCTGAAGA TATGCCAAGC 
TATGGGATTT ATCCTTGGCT CAGACGTAAT GTCGACCTCA AGTTGAAGGC ACTTTTGGAG 
AAATCAATCA GTAGAAGACG GGACACTGAA GCAATACAGA AAGCCAAAAT CCTTTATTCA 
TCTTGCATGA ATGAGAAAGC CATCGAAAAA GCGGATGCCA AGCCACTACT ACATATCCTG 
CGGCACTCAC CTTTCCGCTG GCCTGTGCTC GAATCCAATA TTGGTCCTGA AGGGGTCTGG 
TCAGAGAGAA AGTTCAGCCT TCTGCAGACC CTTGCAACGT TTCGTGGCCA ATACAGCAAT 
TCTGTGTTCA TCCGTTTATA TGTGTCCTCT GATGACAAGA TGTCCAACGA ACACATCTTG 
AAGCTGGACC AAGCGGCGCT CTCCCTAGCC GTGAGGGAAG ACTACCTTGA TAACAGCACG 
GAAGCCAAGT CTTATCGGGA TGCCCTTTAC AAATTCATGG TGGATACTGC TGTGCTTTTG 
GGAGCTAATA GCTCCCGAGC AGAGCATGAC ATGAAGTCGG TGCTTAGGTT GGAAATAAAG 
ATAGCTGAGA TAATGATTCC GCATGAAAAC CGAACCAGTG AAGCCATGTA CAACAAAATG 
AACATCTCCC AGCTGAGTGC TATGATTCCC CAGTTTGACT GGCTGAGCTA CATCAAGAAG 
GTCATCGATG TCAGACTCTA CCCTGAACTG AAAGACATTG GCCCCTCAGA GAACGTGGTG 
GTCCGAGTCC CTCAATACTT TAAGGATTTG TTTAGGATAT TAGGCTCTGA GAGGAAGAAG 
ACCATTGCCA ACTATTTGGT GTGGAGGATG GTTTATTCCA GAATTCCAAA CTTAAGCAGG 
CGCTTTCAGT ATAGGTGGCT GGAGTTCTCA CGGGTAATCC AGGGGACCAC AACTTTGTTG 
CCTCAGTGGG ACAAATGTGT GAACTTCATT GAAAGTACTC TCCCTTATGT CGTTGGAAAA 
ATGTTTGTGA ATGTACACTT CCAGGAAGAC AAGAAGGAAA TGATGGAGGA ATTGATTGAG 
GGTGTTCGAT GGGCCTTTAT TGACATGCTG GAGAAAGAAA ATGAGTGGAT GGATGCAGGG 
ACAAAAAGGA AAGCCAAAGA AAAGGCAAGA GCTGTTTTGG CAAAAGTTGG CTATCCTGAG 
TTTATAATGA ATGATACTCA TATTAATGAA GACCTCAAGG CAATCAAGTT TTCAGAATCT 
GACTACTTTG GCAACGTCCT GCAAACCCGC AAGTATTTAG CACAGACTGA TTTCTTCTGG 
CTGAGAAAAG CTGTTCCCAA AACAGAGTGG TTTACAAATC CAACGACTGT CAATGCCTTC 
TACAGTGCAT CCACCAACCA GATCCGATTT CCAGCAGGAG AACTCCAGAA GCCTTTCTTT 
TGGGGAACAG AATATCCTCG ATCTCTGAGT TATGGTGCTA TAGGAGTAAT TGTTGGACAT 
GAATTTACAC ATGGATTTGA TAATAATGGT CGAAAATATG ATAAAAATGG AAACCTGGAT 
CCTTGGTGGT CTATGGATTC AGAAGAAAAG TTTAAGGAAA AAACAAAGTG CATGATTAAC 
CAGTATAGCA ACTATTATTG GAAGAAAGCT GGCTTAAATG TGAAGGGGAA GAGGACACTG 
GGAGAAAATA TTGCTGATAA TGGAGGGCTG CGACAAGCTT TTAGGGCTTA CAGGAAATGG 
ATAAATGACA AGAGGCAGGG AGTTGAGGAG CCTCTCCTAC CAGGCATTGA ATTCACCAAC 
AACCAGCTTT TCTTCATGAG TTATGCTCAC GTGAGGTGCA ATTCCTACAG ACCAGAAGCT 
GCTCGAGAAC AAATCCAAGC TGGTGCTCAC AGCCCCCCAC AGTTTAGGGT CAATGGTGCA 
GTTAGCAACT TTGAAGAATT CCAGAAGGCT TTTAATTGTC CATCCAATTC CACAATGAAC 
AGAGGCGCAG AATCCTGCCG ACTCTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Establishment of a high throughput EST sequencing system using poly(A) tail-removed cDNA libraries and determination of 36,000 bovine ESTs.
Nucleic acids research29(22)E108(2001 Nov)
Takasuga A,Hirotsune S,Itoh R,Jitohzono A,Suzuki H,Aso H,Sugimoto Y