CEP295 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015469903.1	XP_015325389.1	centrosomal protein of 295 kDa isoform X6
XM_010820789.2	XP_010819091.1	centrosomal protein of 295 kDa isoform X4
XM_024987480.1	XP_024843248.1	centrosomal protein of 295 kDa isoform X3
XM_010820791.3	XP_010819093.1	centrosomal protein of 295 kDa isoform X8
XM_015469902.1	XP_015325388.1	centrosomal protein of 295 kDa isoform X5
XM_010820786.2	XP_010819088.1	centrosomal protein of 295 kDa isoform X3
XM_003588005.5	XP_003588053.2	centrosomal protein of 295 kDa isoform X2
XM_024987482.1	XP_024843250.1	centrosomal protein of 295 kDa isoform X9
XM_010820784.2	XP_010819086.1	centrosomal protein of 295 kDa isoform X1
XM_003588005.4	XP_003588053.2	centrosomal protein of 295 kDa isoform X2
XM_010820791.2	XP_010819093.1	centrosomal protein of 295 kDa isoform X6
XM_015469899.1	XP_015325385.1	centrosomal protein of 295 kDa isoform X1
XM_015469900.1	XP_015325386.1	centrosomal protein of 295 kDa isoform X3
XM_015469901.1	XP_015325387.1	centrosomal protein of 295 kDa isoform X4
XM_010820784.3	XP_010819086.1	centrosomal protein of 295 kDa isoform X1
XM_010820786.3	XP_010819088.1	centrosomal protein of 295 kDa isoform X4
XM_024987481.1	XP_024843249.1	centrosomal protein of 295 kDa isoform X5
XM_010820790.3	XP_010819092.1	centrosomal protein of 295 kDa isoform X7
XM_010826192.2	XP_010824494.2	centrosomal protein of 295 kDa isoform X2
XM_010820790.2	XP_010819092.1	centrosomal protein of 295 kDa isoform X5
XM_010820789.3	XP_010819091.1	centrosomal protein of 295 kDa isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232255	XM_024987480.1 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa232256	XM_024987482.1 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa34402	XM_003588005.5 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa34402	XM_010826192.2 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52309	XM_010820784.3 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52309	XM_015469899.1 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52311	XM_015469900.1 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52311	XM_010820786.3 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52313	XM_024987481.1 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52314	XM_015469901.1 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52314	XM_010820789.3 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52315	XM_010820790.3 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52315	XM_015469902.1 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52316	XM_010820791.3 Latest version!	Bos taurus centrosomal protein 295 (CEP295), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52316	XM_015469903.1 Latest version!	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OBa34402	XM_003588005.4	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52316	XM_010820791.2	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52309	XM_010820784.2	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52311	XM_010820786.2	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa52315	XM_010820790.2	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa52314	XM_010820789.2	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
Hide Old Accession Versions >>

CEP295 ( XM_024987480.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843248.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa232255
Clone ID Related Accession (Same CDS sequence)	XM_024987480.1
Accession Version	XM_024987480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GAAATGAAAA CCACTCAGCC TCTCATAGCT CCAAGTTCAA TTCTACTTCA TCCTCAGGAA 
GAAGCAGTCA GAGTTAGAAT GGCAGCGAGG CAGAAACAGA TAATGGAAAT AGAAGAACAG 
AAGCAAAAGC AGTTGGAATT GCTTGAACAG ATTGAACAAC AGAAGTTAAG ATTAGAAACT 
GATTGCTTCA GAGCTCAGCT GGAAGAAGAA AAAAGAAAAA AAACTCAACA GACTGGGGTC 
AGCACTGCTC CAACATCATA CACCATATGT TCTGATGAAG ATAGGCACAG GCAGATGATT 
CATATTTATC AGCAACAGCT ATTACAGCAA AACAGGTTTC ACAAGCAGTC TGTTGAAACA 
GCCAGGAAGC GATTACTTGA ATATCAGACA ATGTTAAGAG GAAAGTACCC ATCCATGTCA 
GCTACGCCAG TGATACCTGA TTCTGTTACA TCAGTGCCAC CACCAAAATC TGAAAGACCC 
AGCGTTCCAT CAGAACATGA GGATCAAGGT CAGAGACCCA AACTGAGTCC TGACAAACAA 
CCTGTACAGA TCTCCAAATT AGAGCAAGAT TCTCAGGTTT CAAGACAGAG TCGCTTTCCA 
CAAAGACAGG TAGAAACAAC TGAAGCATTA GTCACTTCAG ATGTTTTAGC CAAGCAAGCT 
TTGGAATCAG AAGAACACCA AAAGCAACTC TCACAGACTG AAGTACAACA GAGAGACTAC 
AAATTGGTCG CTAAAGATTC TCAAATGCTT TCAAGGGCTT TATCACATGA TAAGCCACTG 
ACAGTACAGG ATGCTAGAGA AAGAGCTGAA ACATCTGGGG CAACAGCTTT TCAAAGTTTA 
GACTCCCAGC AAGTGTTCTC AGAGAACAAT GAAAGTGTAT CTTCTAAGTT AATTGAACCA 
TCTTCATTCT TACCATTGGT AGCTGAGCAT TCTTTTAGTT CTCTGCCTAC TAAAGTCGAG 
TCTGGAGAAA TCCAGCAGCC CTCTTCAACT GCGAGCAAAG GTGTAGTTTC TGTAAGCCAC 
TCTGTAGCTG GCCAAATGCA GGATAAGTCT TTGCCATTCT CGGAAAATAT CATAGCCCAG 
CAAAGTAATT TGAAGGCTCT CCAAGAACAG TTAGACCTAC AGAAGGAAGT TCTTCGGACA 
AGACAGGAAG CTCAGGAACA ATTGCTTTTG TGTAAACAGA AAGAGTTGGA AGGGCAAACT 
GGCCTTTCTA TATTCCTTCC TTTAGTACCT CTGAATTCAT TTGCTTCACT GCCTTCAGCC 
CAAGCTGAGT CAAGGAGAAT CCAGGAATCT TCTCCAACCA ATAAAGAGAC TGGTGTTTCC 
TCAGGCCATC CTGGGGTCCC ACAACTTCAG GATAGGCTTA ATTTAAAATT TTTCCAAGAA 
CAGTTAAAAA CGCAGAGGGA CAGCCTTCAG GCTAGGCGGG AAGCCCAGGA AGTATTATGT 
GTGCATAAAC AGAATGAGTT GGATGGGAGA GTATGGGCTG AACAGACTGG GTCCTCTTCT 
CTCCCACCTC AAGTAGCTCA GCATACATTT ACTTCACTGC CTTCTGCTGG TCCTCAGTCT 
GGAAAAATCC AGGACCAGTA TTCATCTAAG ACTGAGAAGG AGCATTTCTC AATCCAGACT 
GAAATGCCCA CATCTCAGGA TGGGTCTTTA GGTTTCCCAC AACAGTTCCA ACCTTTGCAC 
GATTGTTTGA AGTTGCTCCA AGAGCAGCTG ATTACACAGA GGGATGCTCT CCAGGCCAGG 
CATGAAGCCC AAGTGGAATT ACTTTCACGT AAACAAAGGG ATTTGGAAGA CGCTAAGTCT 
GTGCAGATGA GTTCTTCGTT CCTGCCAGTG GTCACTCAAC ATTCACTTGC TTCACAAGTT 
TCAGCTAAAA CTGAGCCTAG GAGAATTCAG AACTTTTATT TCTCTGAGAG GGATAGTGTT 
ATTCCCTCAA ATAATTTGGT AATGCCAGCA TCTCAGGATG CGTCTCTTAG TTTTCCACAG 
TATAGTCTGC CACGGCAGGA AAATTTAACA GCACTCCACA ATCAGTCACA CGTTCAGAGG 
GTAATACTTG GCGATAAACA AGAAACTCAG GAATTTGTAC ACAAACAAAG TGAATTAGAA 
AAAATTTTCC CTTCTGAACA GACTGGCACT TCTTTGTCTC AGGGAGCTGA ATCTGAAAGA 
TGCCAGGAAT ACATGTCATT CAGGAGTGAC AGTACTGTTC CCTTGAGCCA CTGGAAGATC 
CCAAGATCTC AGGAAAGACT TCTGGGATTT TCACGACATA CACAACCTCT GCAAGGTAAT 
TTGGAAGGAC AGCAAGAACA GTTAGACAGA GAAGAGGAGG CCCTTCAGTT CAGCCAGAGA 
TCCCAAGGGA ATGTATCTTC TGGACAGATT TGCCCCTCGT TCACACCCCG GTTAGGGCAG 
CTCTCTCTTA CTTCATTGCC TTCTGCTGAA TCTCGGGAAC CTCTTTCAGC AGAGAGTGAG 
AGTAGAACTC CTTCAAGCCG TTTTCACATC CCGGTGTTGC AGGATAGACT TTGGAAGTTA 
TCACAGCTTA TACAGCCTCA GCAAGATAAC CTGAAGTCAC TCCAAGAACA GTTAGCTACA 
CAGAGGGAAG CCATCATTCA GTCTAGGCAG GAAGCTCAGC AAGAATTTCT TTTGCACAAA 
CAGGGTGAGT GGAAGGGAAG AGTGTCTCCA GAGCAGGTTG GCACCTCCTC CTTCCTACCC 
CAAGTCATAC AGCCTCTTGC TCCATTACCT CCTAGTAAAG CTGGTAGAAT CCAAGAACCT 
TGTTCAACTA AGAGTGATAA TATAGTCTCC TCAGGTCATT CTGAGATACC AAGGTTGCCT 
GATAGACTTT TAGGTTTACC ACAGCCTGTT TTGCCTCAAC AAGATTATCC AGTCACATTT 
CAACAAGAAC ATTTGTATGC CCAGACAAAC CCCCTTCCAT GTAGCGAGAA AATCCAAAGA 
GAGTTGGTTT TGCCCAGACA ATATACACTT GAGGGAAAGT CACCTAAGCA TTTTGTCCAA 
CCTCACCATG GTGATTTAAA GGCCTATCAA CGGCAGTTAG ATATACAGAG GGAAGTCCAA 
GAAGAATTCC TTTTGCAAAC ACTAAGTAAA TTGGAAAAAG GGGTCTCAGC TGAGCAGACC 
ACCACCTCTT CATCCTTATC CCACGTAGCA CTGCCTGTTG CTGACTCTAA AGGAATGCCA 
AAGTGTTTTC CAGCCAGAAG TGACCTTGCT GTTTCCTCAA GTCATCCTGA GATTCTTAGA 
CCTCAGGAGA GGCCTTTGCA TTTATCCCAG GCTGTTTTGC CTCAGCAAGA CCATGTGTCC 
ACACAGTTGG GCCTTCAGGG TGAAGTGCTA CGTTTTAGTG AAAAGGCCCA GGAAGAACTG 
CTAAGAGGCA ATCACACAAA ACTGATTGAA GGTGATGCTT CTGGGCAGCC TGTGCCCTCT 
TTGTTTCTAC CCAAGGAAAG AGAACATTCG TTTATTCCAC TACCTTTTGC TGAGGTAAAA 
TCTCAAAACA TACGTGAATT GTATTCAGCC AAGATTGAAT GTGCAGCTCC CTCTAGTGCT 
TCTGTGAGCC CAAGACTTCA AGATAGAATT TTGAGTTTTT CACAACCCAT CTTTGCTCAG 
CAAGATAACT GGGGACTTCA GAAGCAGTTG GAGCTACAAA AAGAAGTTCT GCCTTTTAGT 
CAGAAAGCCC AAGAAGTATT GCTTGTACCA ACACAGACAG CCTTGCAGCA ACAGACACAC 
AAACATCGGG AGACTCTGAA GGATTTCTTC AAAGACAGCC AGGAAAGTAA GCCCACAGTT 
GAAAATGATT TTGAAACCCA GAAGCTCCAA GAATGGCTTC CTCATCTCCA CGATCAAAAA 
AACATTGGTC CTGCAGACAG GAGCAACTGT GATGATTATC AGCTCCTTTC AGAAGGTGTG 
CATCAGGACA AAGAACTGGG TAGGAAATCC TCAAAACCAC CTGTAGTAAA AATAAGAGGT 
GGATTGGACT TGAACCAACA TGAACTTAGT GCCATACAAG AGGTAGAATC ACCAACAAGT 
GGCAGAACTT CTATACTAGG TAAACCTGAT TTTTATCAAG ACAGAGACCC CCTAAGGGTC 
TCAATAAGCC GAGAACAAAG TTTTCTTGGG AGCCCTCTGG ACCGTGATCC ATTTGGTCAT 
CTTCACCCAG TTGCTCAGGA GAACATCTGT GGTGATGACT CTGATAAAGC AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843248.1
CDS	77..7825
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_024987480.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987480.1

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GAAATGAAAA CCACTCAGCC TCTCATAGCT CCAAGTTCAA TTCTACTTCA TCCTCAGGAA 
GAAGCAGTCA GAGTTAGAAT GGCAGCGAGG CAGAAACAGA TAATGGAAAT AGAAGAACAG 
AAGCAAAAGC AGTTGGAATT GCTTGAACAG ATTGAACAAC AGAAGTTAAG ATTAGAAACT 
GATTGCTTCA GAGCTCAGCT GGAAGAAGAA AAAAGAAAAA AAACTCAACA GACTGGGGTC 
AGCACTGCTC CAACATCATA CACCATATGT TCTGATGAAG ATAGGCACAG GCAGATGATT 
CATATTTATC AGCAACAGCT ATTACAGCAA AACAGGTTTC ACAAGCAGTC TGTTGAAACA 
GCCAGGAAGC GATTACTTGA ATATCAGACA ATGTTAAGAG GAAAGTACCC ATCCATGTCA 
GCTACGCCAG TGATACCTGA TTCTGTTACA TCAGTGCCAC CACCAAAATC TGAAAGACCC 
AGCGTTCCAT CAGAACATGA GGATCAAGGT CAGAGACCCA AACTGAGTCC TGACAAACAA 
CCTGTACAGA TCTCCAAATT AGAGCAAGAT TCTCAGGTTT CAAGACAGAG TCGCTTTCCA 
CAAAGACAGG TAGAAACAAC TGAAGCATTA GTCACTTCAG ATGTTTTAGC CAAGCAAGCT 
TTGGAATCAG AAGAACACCA AAAGCAACTC TCACAGACTG AAGTACAACA GAGAGACTAC 
AAATTGGTCG CTAAAGATTC TCAAATGCTT TCAAGGGCTT TATCACATGA TAAGCCACTG 
ACAGTACAGG ATGCTAGAGA AAGAGCTGAA ACATCTGGGG CAACAGCTTT TCAAAGTTTA 
GACTCCCAGC AAGTGTTCTC AGAGAACAAT GAAAGTGTAT CTTCTAAGTT AATTGAACCA 
TCTTCATTCT TACCATTGGT AGCTGAGCAT TCTTTTAGTT CTCTGCCTAC TAAAGTCGAG 
TCTGGAGAAA TCCAGCAGCC CTCTTCAACT GCGAGCAAAG GTGTAGTTTC TGTAAGCCAC 
TCTGTAGCTG GCCAAATGCA GGATAAGTCT TTGCCATTCT CGGAAAATAT CATAGCCCAG 
CAAAGTAATT TGAAGGCTCT CCAAGAACAG TTAGACCTAC AGAAGGAAGT TCTTCGGACA 
AGACAGGAAG CTCAGGAACA ATTGCTTTTG TGTAAACAGA AAGAGTTGGA AGGGCAAACT 
GGCCTTTCTA TATTCCTTCC TTTAGTACCT CTGAATTCAT TTGCTTCACT GCCTTCAGCC 
CAAGCTGAGT CAAGGAGAAT CCAGGAATCT TCTCCAACCA ATAAAGAGAC TGGTGTTTCC 
TCAGGCCATC CTGGGGTCCC ACAACTTCAG GATAGGCTTA ATTTAAAATT TTTCCAAGAA 
CAGTTAAAAA CGCAGAGGGA CAGCCTTCAG GCTAGGCGGG AAGCCCAGGA AGTATTATGT 
GTGCATAAAC AGAATGAGTT GGATGGGAGA GTATGGGCTG AACAGACTGG GTCCTCTTCT 
CTCCCACCTC AAGTAGCTCA GCATACATTT ACTTCACTGC CTTCTGCTGG TCCTCAGTCT 
GGAAAAATCC AGGACCAGTA TTCATCTAAG ACTGAGAAGG AGCATTTCTC AATCCAGACT 
GAAATGCCCA CATCTCAGGA TGGGTCTTTA GGTTTCCCAC AACAGTTCCA ACCTTTGCAC 
GATTGTTTGA AGTTGCTCCA AGAGCAGCTG ATTACACAGA GGGATGCTCT CCAGGCCAGG 
CATGAAGCCC AAGTGGAATT ACTTTCACGT AAACAAAGGG ATTTGGAAGA CGCTAAGTCT 
GTGCAGATGA GTTCTTCGTT CCTGCCAGTG GTCACTCAAC ATTCACTTGC TTCACAAGTT 
TCAGCTAAAA CTGAGCCTAG GAGAATTCAG AACTTTTATT TCTCTGAGAG GGATAGTGTT 
ATTCCCTCAA ATAATTTGGT AATGCCAGCA TCTCAGGATG CGTCTCTTAG TTTTCCACAG 
TATAGTCTGC CACGGCAGGA AAATTTAACA GCACTCCACA ATCAGTCACA CGTTCAGAGG 
GTAATACTTG GCGATAAACA AGAAACTCAG GAATTTGTAC ACAAACAAAG TGAATTAGAA 
AAAATTTTCC CTTCTGAACA GACTGGCACT TCTTTGTCTC AGGGAGCTGA ATCTGAAAGA 
TGCCAGGAAT ACATGTCATT CAGGAGTGAC AGTACTGTTC CCTTGAGCCA CTGGAAGATC 
CCAAGATCTC AGGAAAGACT TCTGGGATTT TCACGACATA CACAACCTCT GCAAGGTAAT 
TTGGAAGGAC AGCAAGAACA GTTAGACAGA GAAGAGGAGG CCCTTCAGTT CAGCCAGAGA 
TCCCAAGGGA ATGTATCTTC TGGACAGATT TGCCCCTCGT TCACACCCCG GTTAGGGCAG 
CTCTCTCTTA CTTCATTGCC TTCTGCTGAA TCTCGGGAAC CTCTTTCAGC AGAGAGTGAG 
AGTAGAACTC CTTCAAGCCG TTTTCACATC CCGGTGTTGC AGGATAGACT TTGGAAGTTA 
TCACAGCTTA TACAGCCTCA GCAAGATAAC CTGAAGTCAC TCCAAGAACA GTTAGCTACA 
CAGAGGGAAG CCATCATTCA GTCTAGGCAG GAAGCTCAGC AAGAATTTCT TTTGCACAAA 
CAGGGTGAGT GGAAGGGAAG AGTGTCTCCA GAGCAGGTTG GCACCTCCTC CTTCCTACCC 
CAAGTCATAC AGCCTCTTGC TCCATTACCT CCTAGTAAAG CTGGTAGAAT CCAAGAACCT 
TGTTCAACTA AGAGTGATAA TATAGTCTCC TCAGGTCATT CTGAGATACC AAGGTTGCCT 
GATAGACTTT TAGGTTTACC ACAGCCTGTT TTGCCTCAAC AAGATTATCC AGTCACATTT 
CAACAAGAAC ATTTGTATGC CCAGACAAAC CCCCTTCCAT GTAGCGAGAA AATCCAAAGA 
GAGTTGGTTT TGCCCAGACA ATATACACTT GAGGGAAAGT CACCTAAGCA TTTTGTCCAA 
CCTCACCATG GTGATTTAAA GGCCTATCAA CGGCAGTTAG ATATACAGAG GGAAGTCCAA 
GAAGAATTCC TTTTGCAAAC ACTAAGTAAA TTGGAAAAAG GGGTCTCAGC TGAGCAGACC 
ACCACCTCTT CATCCTTATC CCACGTAGCA CTGCCTGTTG CTGACTCTAA AGGAATGCCA 
AAGTGTTTTC CAGCCAGAAG TGACCTTGCT GTTTCCTCAA GTCATCCTGA GATTCTTAGA 
CCTCAGGAGA GGCCTTTGCA TTTATCCCAG GCTGTTTTGC CTCAGCAAGA CCATGTGTCC 
ACACAGTTGG GCCTTCAGGG TGAAGTGCTA CGTTTTAGTG AAAAGGCCCA GGAAGAACTG 
CTAAGAGGCA ATCACACAAA ACTGATTGAA GGTGATGCTT CTGGGCAGCC TGTGCCCTCT 
TTGTTTCTAC CCAAGGAAAG AGAACATTCG TTTATTCCAC TACCTTTTGC TGAGGTAAAA 
TCTCAAAACA TACGTGAATT GTATTCAGCC AAGATTGAAT GTGCAGCTCC CTCTAGTGCT 
TCTGTGAGCC CAAGACTTCA AGATAGAATT TTGAGTTTTT CACAACCCAT CTTTGCTCAG 
CAAGATAACT GGGGACTTCA GAAGCAGTTG GAGCTACAAA AAGAAGTTCT GCCTTTTAGT 
CAGAAAGCCC AAGAAGTATT GCTTGTACCA ACACAGACAG CCTTGCAGCA ACAGACACAC 
AAACATCGGG AGACTCTGAA GGATTTCTTC AAAGACAGCC AGGAAAGTAA GCCCACAGTT 
GAAAATGATT TTGAAACCCA GAAGCTCCAA GAATGGCTTC CTCATCTCCA CGATCAAAAA 
AACATTGGTC CTGCAGACAG GAGCAACTGT GATGATTATC AGCTCCTTTC AGAAGGTGTG 
CATCAGGACA AAGAACTGGG TAGGAAATCC TCAAAACCAC CTGTAGTAAA AATAAGAGGT 
GGATTGGACT TGAACCAACA TGAACTTAGT GCCATACAAG AGGTAGAATC ACCAACAAGT 
GGCAGAACTT CTATACTAGG TAAACCTGAT TTTTATCAAG ACAGAGACCC CCTAAGGGTC 
TCAATAAGCC GAGAACAAAG TTTTCTTGGG AGCCCTCTGG ACCGTGATCC ATTTGGTCAT 
CTTCACCCAG TTGCTCAGGA GAACATCTGT GGTGATGACT CTGATAAAGC AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_024987482.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843250.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X12, mRNA.

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CloneID	OBa232256
Clone ID Related Accession (Same CDS sequence)	XM_024987482.1
Accession Version	XM_024987482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843250.1
CDS	61..6447
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQH

Target ORF information:

RefSeq Version	XM_024987482.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987482.1

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_003588005.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003588053.2 Bos taurus centrosomal protein 295 (CEP295), transcript variant X2, mRNA.

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CloneID	OBa34402
Clone ID Related Accession (Same CDS sequence)	XM_003588005.5 , XM_003588005.4 , XM_010826192.2
Accession Version	XM_003588005.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003588005.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003588053.2
CDS	61..7899
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKKTLEKLRARNIC

Target ORF information:

RefSeq Version	XM_003588005.5
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588005.5

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_003588005.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003588053.2 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.

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CloneID	OBa34402
Clone ID Related Accession (Same CDS sequence)	XM_003588005.5 , XM_003588005.4 , XM_010826192.2
Accession Version	XM_003588005.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003588005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003588053.2
CDS	28..7866
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKKTLEKLRARNIC

Target ORF information:

RefSeq Version	XM_003588005.4
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003588005.4

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010826192.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824494.2 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.

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CloneID	OBa34402
Clone ID Related Accession (Same CDS sequence)	XM_003588005.5 , XM_003588005.4 , XM_010826192.2
Accession Version	XM_010826192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824494.2
CDS	28..7866
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKKTLEKLRARNIC

Target ORF information:

RefSeq Version	XM_010826192.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826192.2

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
AAAACATTAG AGAAGCTTCG AGCCAGAAAT ATATGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820784.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819086.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.

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CloneID	OBa52309
Clone ID Related Accession (Same CDS sequence)	XM_010820784.2 , XM_015469899.1 , XM_010820784.3
Accession Version	XM_010820784.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819086.1
CDS	28..7869
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820784.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820784.2

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_015469899.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325385.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.

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CloneID	OBa52309
Clone ID Related Accession (Same CDS sequence)	XM_010820784.2 , XM_015469899.1 , XM_010820784.3
Accession Version	XM_015469899.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325385.1
CDS	28..7869
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_015469899.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469899.1

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820784.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819086.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X1, mRNA.

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CloneID	OBa52309
Clone ID Related Accession (Same CDS sequence)	XM_010820784.2 , XM_015469899.1 , XM_010820784.3
Accession Version	XM_010820784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819086.1
CDS	77..7918
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820784.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820784.3

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GAGGTCAGGT TCTTCCTGTG AAAGCCTCCG TGCTACTCCA 
TCCCCCAAAA GTATGGCTTC TTTTACAGCA CTGAGGAGGG CCAGCCTGCA TTCTTCTAAC 
ACCAGTCTGA ACCAGCAGCC TGACCCTAAC TTGGCTTGTG ACACAGCTCA GAGTTTTGCT 
GTAGAAGATA TTGAGGGGTC TGAACAATCT TTTCGACAAC TTCGACCAGA ATATTCTTCA 
CAGGAAGGGA GCCAGCATGC CGATCTACCA AGCATTTTTA GCATTGAAGC GAGAGCTTCT 
TCCCAAGATG TGGAAAATCA GAACTGTTCC TCTGAACATG AACTATTACA AAACAAGCAA 
AAAAGTGTTC ATTTCCAACT CTCAGTAGGA AATTTGCAAA GTTCAGTCTT CAATTCATCT 
GGTGAGGCTA ATGTATTTCA TCAGTTAAAT CTACAGCATG GCACTACATG TGGTTCTACC 
TCTAGTGAGT GGTCAATAAA AGACCAACCA GAAGGCAGAA AAGAAAGACT GGGTTTTGAA 
AAACTATCAG AAAGACTTGA TACAGTGTTA CAAGGTCAAG GACTCACTGA TGATAAAAAA 
GAAACCTGTG ATTTAAATCC ACACATAGAG GAAACTGATT CTCATTTATG TGTAAGGACA 
GTAGAGATGG GAACTTCAAT TCAGATACCA TATTCACTCA CTGTTCAGAA TGAAAAATGT 
CTTCAGGACT CAACTATGGC AGGAATTCCA ACAATCGTAG GAAACCCATC TCAACTTGCA 
CAATCAGAGC TCTTTGTAAA TTCTGGATCA TTTTCATTAC AGAGATCTAT TCCAGTCTGG 
GAAACAGAAT CTGGCCATGG TATAATGGAA GAACCAGAAC TTACATTGAC AAGCACCAGT 
GATATCAGTA TTGCTGAAAT GGATTTTGCA AACTTAACCC TAGAAGAAAA GAGAGAAAAT 
GAGGCAAAGA GCTGTTTTCA GGTGAGTGAA TTTCTGCCTC TTCTGTCAGA AACAGAAAAC 
TCAGATTATC CAGCCATGTC AGAACATCCA CTGGAGAGAC CAACTGTGTG TGGAGAAACC 
TTTTCAAGGT GTACAGCTGC ACCAGAGAGC TTACAAGAAG CATTCATAAA AAGAAAAAAG 
GCATTTATAG AGAGATCCTC ACAGAGACAG AAAGAGATAA AGGACAAAGT TCATGCCTCT 
GGATATTCTC AAACCAAAAC AATAACAGAG AAAACTACAG GTTCCTTTGT GAGTGACCTA 
AAGGGTATGA GTAAAGTTAG GGTGTCGCTT CCTGAAGATA AAAAGACTGC ACAACCCCAT 
ACGCACGAAA GGGCAGTAGG ATTATACCTT CAATTAGCTG AAATGAGAAA GCAGAAGGAA 
GACAAAGCAA AGCAAGATGC ACATGCCCAA AACAGAGCAA GGACAAAAGA ATTTCATAAG 
GTAAGCAGCA TCCCATACAT ACCCCCAACT TTTCATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820786.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819088.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.

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CloneID	OBa52311
Clone ID Related Accession (Same CDS sequence)	XM_010820786.2 , XM_015469900.1 , XM_010820786.3
Accession Version	XM_010820786.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819088.1
CDS	29..7717
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820786.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820786.2

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_015469900.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325386.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.

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CloneID	OBa52311
Clone ID Related Accession (Same CDS sequence)	XM_010820786.2 , XM_015469900.1 , XM_010820786.3
Accession Version	XM_015469900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325386.1
CDS	29..7717
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_015469900.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469900.1

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820786.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819088.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X4, mRNA.

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CloneID	OBa52311
Clone ID Related Accession (Same CDS sequence)	XM_010820786.2 , XM_015469900.1 , XM_010820786.3
Accession Version	XM_010820786.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820786.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819088.1
CDS	61..7749
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820786.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820786.3

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGTCAAAGTC 
AAGGAGGCTG TGGTTGAGAA TTATGCAGTA TTAAGCTATG CTGTGGAGAG AGAAGGCCAT 
ACGTACCTGG ATCCAAATGT GAAGTTAGAT GTTAAGGCTG AAACACAAGA AATTTATCAT 
GAGCCACTAT CTTCAATAAC TGTTTCCACT GGGAGTTTTT TAAGTTACGA AAACATAGAT 
TTGAGCCTTA CAGAGCCAGA GTCATTCCCA GAGCATGTGG ACCATAGGGA ACAAGAACCT 
CCTGCCGGTA AAGAAGAGGA AACAAATGTT TTAAGTTCTA GAGTTCCTTC AACACAAGAT 
ATTTATCAAA GACAGGACTC TCAGGACGTC CATAAACCTC TTTTATGTGC AGTGGAAACA 
TTGACATCTG GTCAGACACA CATTCAACAG ATGAGAGACA ATTACATTAA TGAAACAAAT 
TTGATGACTG AAAAAACAGA CTTTCGGGTT GACTTTGACT TTCCAGAATT GGAGCACAAT 
TTTCCAAATT TGCATCGTCA GCTATTTAAA CCCTTAGAAC CACATCCAGA TTTTGATTTG 
TTATCATGTT TCTCTGGGAT TTCTCAAGAC AGTAAAGACT TTAACCAGAG GTCAGGTTCT 
TCCTGTGAAA GCCTCCGTGC TACTCCATCC CCCAAAAGTA TGGCTTCTTT TACAGCACTG 
AGGAGGGCCA GCCTGCATTC TTCTAACACC AGTCTGAACC AGCAGCCTGA CCCTAACTTG 
GCTTGTGACA CAGCTCAGAG TTTTGCTGTA GAAGATATTG AGGGGTCTGA ACAATCTTTT 
CGACAACTTC GACCAGAATA TTCTTCACAG GAAGGGAGCC AGCATGCCGA TCTACCAAGC 
ATTTTTAGCA TTGAAGCGAG AGCTTCTTCC CAAGATGTGG AAAATCAGAA CTGTTCCTCT 
GAACATGAAC TATTACAAAA CAAGCAAAAA AGTGTTCATT TCCAACTCTC AGTAGGAAAT 
TTGCAAAGTT CAGTCTTCAA TTCATCTGGT GAGGCTAATG TATTTCATCA GTTAAATCTA 
CAGCATGGCA CTACATGTGG TTCTACCTCT AGTGAGTGGT CAATAAAAGA CCAACCAGAA 
GGCAGAAAAG AAAGACTGGG TTTTGAAAAA CTATCAGAAA GACTTGATAC AGTGTTACAA 
GGTCAAGGAC TCACTGATGA TAAAAAAGAA ACCTGTGATT TAAATCCACA CATAGAGGAA 
ACTGATTCTC ATTTATGTGT AAGGACAGTA GAGATGGGAA CTTCAATTCA GATACCATAT 
TCACTCACTG TTCAGAATGA AAAATGTCTT CAGGACTCAA CTATGGCAGG AATTCCAACA 
ATCGTAGGAA ACCCATCTCA ACTTGCACAA TCAGAGCTCT TTGTAAATTC TGGATCATTT 
TCATTACAGA GATCTATTCC AGTCTGGGAA ACAGAATCTG GCCATGGTAT AATGGAAGAA 
CCAGAACTTA CATTGACAAG CACCAGTGAT ATCAGTATTG CTGAAATGGA TTTTGCAAAC 
TTAACCCTAG AAGAAAAGAG AGAAAATGAG GCAAAGAGCT GTTTTCAGGT GAGTGAATTT 
CTGCCTCTTC TGTCAGAAAC AGAAAACTCA GATTATCCAG CCATGTCAGA ACATCCACTG 
GAGAGACCAA CTGTGTGTGG AGAAACCTTT TCAAGGTGTA CAGCTGCACC AGAGAGCTTA 
CAAGAAGCAT TCATAAAAAG AAAAAAGGCA TTTATAGAGA GATCCTCACA GAGACAGAAA 
GAGATAAAGG ACAAAGTTCA TGCCTCTGGA TATTCTCAAA CCAAAACAAT AACAGAGAAA 
ACTACAGGTT CCTTTGTGAG TGACCTAAAG GGTATGAGTA AAGTTAGGGT GTCGCTTCCT 
GAAGATAAAA AGACTGCACA ACCCCATACG CACGAAAGGG CAGTAGGATT ATACCTTCAA 
TTAGCTGAAA TGAGAAAGCA GAAGGAAGAC AAAGCAAAGC AAGATGCACA TGCCCAAAAC 
AGAGCAAGGA CAAAAGAATT TCATAAGGTA AGCAGCATCC CATACATACC CCCAACTTTT 
CATGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_024987481.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843249.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X6, mRNA.

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CloneID	OBa52313
Clone ID Related Accession (Same CDS sequence)	XM_024987481.1
Accession Version	XM_024987481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATATAA AAGCTCGAAA AGAAGCTCTG CTTGTGGAAA AGGAAAGATC TGCCAAAATT 
GCAAGTCTGC CACCCCCTCC TCCAGCTCTC TTTGAGAATG TTGAAGTAAA GAAAACTAAC 
GTGAAGACGA ACAGTTCTAC CTACCATCAT CTTTGTACTT TTGTGAATAG AGAGGTGGAC 
ACAAAGCAGC CAGATCCTCA TTTGGCTGCT GAAGAAGAAG CTAAAAGATT AGAAGAACTA 
CAGAAGCAGG CAGCGCAAGA GAAAATGGAA CAGAGTGAGA AGGCACATGC CCGGGGATTC 
CAAGCAATGA AAAAGATCCA TTTGGCTCAA AATCAAGAGA AACTAATGAA AGAACTCCAG 
CAGCTCCAGC AAGAGGACCT GGCACGCAGG AGACTGATGG TAGCACGGAT GCCGCCACAA 
CTAGTGGAAC TTCCATACAA ACGCAGTGAA ATGAAAGAAG ATTGGCAGAG AGAGCTAGAA 
TTTGCCTTTG AAGACATGTA CAATGCAGAC AGAAAGGTGA AAGGAAACCT GATTTTGCAC 
CTTGAACCAG AGCCTCTGCC TGCTGTGATT GATCAGATCC AAGACGAAGA ACTGGACCTC 
TCAATGGAAC ATGAAAGTTT AGGGGAGACT GAAAACATTC CAATGACAGA AGCTGAAACA 
GTACATTCTA GTGAAGCAGA CGTTCCCTCA GCAGTGAAGA CCCACCAGAT TCCTTCAAAA 
ATTCTTTTTA AAAAATTATT AAATAAGATC CGAAGCCAAA AATCTCTCTG GACAATTAAA 
TCCGTGTCTG AAGAGGAGGG TGAAATGATT ACAACTGTCA GTGAAGCTGA AAGTAAAGCC 
CTGACAGTTG AATCAGGAGC AACTGTGAGT GAAGACAGAA CATTATCCTC TGGGCAGGAA 
CAAGTTGTTG AAAGTGACAT GCTAACAATT GACTCTGGAC CACTTACTAG TGAAGATAAA 
CCACTTTCAT TTAGTACTGA CTCTGAAAAG GAACAAGAAA TGAAAACCAC TCAGCCTCTC 
ATAGCTCCAA GTTCAATTCT ACTTCATCCT CAGGAAGAAG CAGTCAGAGT TAGAATGGCA 
GCGAGGCAGA AACAGATAAT GGAAATAGAA GAACAGAAGC AAAAGCAGTT GGAATTGCTT 
GAACAGATTG AACAACAGAA GTTAAGATTA GAAACTGATT GCTTCAGAGC TCAGCTGGAA 
GAAGAAAAAA GAAAAAAAAC TCAACAGACT GGGGTCAGCA CTGCTCCAAC ATCATACACC 
ATATGTTCTG ATGAAGATAG GCACAGGCAG ATGATTCATA TTTATCAGCA ACAGCTATTA 
CAGCAAAACA GGTTTCACAA GCAGTCTGTT GAAACAGCCA GGAAGCGATT ACTTGAATAT 
CAGACAATGT TAAGAGGAAA GTACCCATCC ATGTCAGCTA CGCCAGTGAT ACCTGATTCT 
GTTACATCAG TGCCACCACC AAAATCTGAA AGACCCAGCG TTCCATCAGA ACATGAGGAT 
CAAGGTCAGA GACCCAAACT GAGTCCTGAC AAACAACCTG TACAGATCTC CAAATTAGAG 
CAAGATTCTC AGGTTTCAAG ACAGAGTCGC TTTCCACAAA GACAGGTAGA AACAACTGAA 
GCATTAGTCA CTTCAGATGT TTTAGCCAAG CAAGCTTTGG AATCAGAAGA ACACCAAAAG 
CAACTCTCAC AGACTGAAGT ACAACAGAGA GACTACAAAT TGGTCGCTAA AGATTCTCAA 
ATGCTTTCAA GGGCTTTATC ACATGATAAG CCACTGACAG TACAGGATGC TAGAGAAAGA 
GCTGAAACAT CTGGGGCAAC AGCTTTTCAA AGTTTAGACT CCCAGCAAGT GTTCTCAGAG 
AACAATGAAA GTGTATCTTC TAAGTTAATT GAACCATCTT CATTCTTACC ATTGGTAGCT 
GAGCATTCTT TTAGTTCTCT GCCTACTAAA GTCGAGTCTG GAGAAATCCA GCAGCCCTCT 
TCAACTGCGA GCAAAGGTGT AGTTTCTGTA AGCCACTCTG TAGCTGGCCA AATGCAGGAT 
AAGTCTTTGC CATTCTCGGA AAATATCATA GCCCAGCAAA GTAATTTGAA GGCTCTCCAA 
GAACAGTTAG ACCTACAGAA GGAAGTTCTT CGGACAAGAC AGGAAGCTCA GGAACAATTG 
CTTTTGTGTA AACAGAAAGA GTTGGAAGGG CAAACTGGCC TTTCTATATT CCTTCCTTTA 
GTACCTCTGA ATTCATTTGC TTCACTGCCT TCAGCCCAAG CTGAGTCAAG GAGAATCCAG 
GAATCTTCTC CAACCAATAA AGAGACTGGT GTTTCCTCAG GCCATCCTGG GGTCCCACAA 
CTTCAGGATA GGCTTAATTT AAAATTTTTC CAAGAACAGT TAAAAACGCA GAGGGACAGC 
CTTCAGGCTA GGCGGGAAGC CCAGGAAGTA TTATGTGTGC ATAAACAGAA TGAGTTGGAT 
GGGAGAGTAT GGGCTGAACA GACTGGGTCC TCTTCTCTCC CACCTCAAGT AGCTCAGCAT 
ACATTTACTT CACTGCCTTC TGCTGGTCCT CAGTCTGGAA AAATCCAGGA CCAGTATTCA 
TCTAAGACTG AGAAGGAGCA TTTCTCAATC CAGACTGAAA TGCCCACATC TCAGGATGGG 
TCTTTAGGTT TCCCACAACA GTTCCAACCT TTGCACGATT GTTTGAAGTT GCTCCAAGAG 
CAGCTGATTA CACAGAGGGA TGCTCTCCAG GCCAGGCATG AAGCCCAAGT GGAATTACTT 
TCACGTAAAC AAAGGGATTT GGAAGACGCT AAGTCTGTGC AGATGAGTTC TTCGTTCCTG 
CCAGTGGTCA CTCAACATTC ACTTGCTTCA CAAGTTTCAG CTAAAACTGA GCCTAGGAGA 
ATTCAGAACT TTTATTTCTC TGAGAGGGAT AGTGTTATTC CCTCAAATAA TTTGGTAATG 
CCAGCATCTC AGGATGCGTC TCTTAGTTTT CCACAGTATA GTCTGCCACG GCAGGAAAAT 
TTAACAGCAC TCCACAATCA GTCACACGTT CAGAGGGTAA TACTTGGCGA TAAACAAGAA 
ACTCAGGAAT TTGTACACAA ACAAAGTGAA TTAGAAAAAA TTTTCCCTTC TGAACAGACT 
GGCACTTCTT TGTCTCAGGG AGCTGAATCT GAAAGATGCC AGGAATACAT GTCATTCAGG 
AGTGACAGTA CTGTTCCCTT GAGCCACTGG AAGATCCCAA GATCTCAGGA AAGACTTCTG 
GGATTTTCAC GACATACACA ACCTCTGCAA GGTAATTTGG AAGGACAGCA AGAACAGTTA 
GACAGAGAAG AGGAGGCCCT TCAGTTCAGC CAGAGATCCC AAGGGAATGT ATCTTCTGGA 
CAGATTTGCC CCTCGTTCAC ACCCCGGTTA GGGCAGCTCT CTCTTACTTC ATTGCCTTCT 
GCTGAATCTC GGGAACCTCT TTCAGCAGAG AGTGAGAGTA GAACTCCTTC AAGCCGTTTT 
CACATCCCGG TGTTGCAGGA TAGACTTTGG AAGTTATCAC AGCTTATACA GCCTCAGCAA 
GATAACCTGA AGTCACTCCA AGAACAGTTA GCTACACAGA GGGAAGCCAT CATTCAGTCT 
AGGCAGGAAG CTCAGCAAGA ATTTCTTTTG CACAAACAGG GTGAGTGGAA GGGAAGAGTG 
TCTCCAGAGC AGGTTGGCAC CTCCTCCTTC CTACCCCAAG TCATACAGCC TCTTGCTCCA 
TTACCTCCTA GTAAAGCTGG TAGAATCCAA GAACCTTGTT CAACTAAGAG TGATAATATA 
GTCTCCTCAG GTCATTCTGA GATACCAAGG TTGCCTGATA GACTTTTAGG TTTACCACAG 
CCTGTTTTGC CTCAACAAGA TTATCCAGTC ACATTTCAAC AAGAACATTT GTATGCCCAG 
ACAAACCCCC TTCCATGTAG CGAGAAAATC CAAAGAGAGT TGGTTTTGCC CAGACAATAT 
ACACTTGAGG GAAAGTCACC TAAGCATTTT GTCCAACCTC ACCATGGTGA TTTAAAGGCC 
TATCAACGGC AGTTAGATAT ACAGAGGGAA GTCCAAGAAG AATTCCTTTT GCAAACACTA 
AGTAAATTGG AAAAAGGGGT CTCAGCTGAG CAGACCACCA CCTCTTCATC CTTATCCCAC 
GTAGCACTGC CTGTTGCTGA CTCTAAAGGA ATGCCAAAGT GTTTTCCAGC CAGAAGTGAC 
CTTGCTGTTT CCTCAAGTCA TCCTGAGATT CTTAGACCTC AGGAGAGGCC TTTGCATTTA 
TCCCAGGCTG TTTTGCCTCA GCAAGACCAT GTGTCCACAC AGTTGGGCCT TCAGGGTGAA 
GTGCTACGTT TTAGTGAAAA GGCCCAGGAA GAACTGCTAA GAGGCAATCA CACAAAACTG 
ATTGAAGGTG ATGCTTCTGG GCAGCCTGTG CCCTCTTTGT TTCTACCCAA GGAAAGAGAA 
CATTCGTTTA TTCCACTACC TTTTGCTGAG GTAAAATCTC AAAACATACG TGAATTGTAT 
TCAGCCAAGA TTGAATGTGC AGCTCCCTCT AGTGCTTCTG TGAGCCCAAG ACTTCAAGAT 
AGAATTTTGA GTTTTTCACA ACCCATCTTT GCTCAGCAAG ATAACTGGGG ACTTCAGAAG 
CAGTTGGAGC TACAAAAAGA AGTTCTGCCT TTTAGTCAGA AAGCCCAAGA AGTATTGCTT 
GTACCAACAC AGACAGCCTT GCAGCAACAG ACACACAAAC ATCGGGAGAC TCTGAAGGAT 
TTCTTCAAAG ACAGCCAGGA AAGTAAGCCC ACAGTTGAAA ATGATTTTGA AACCCAGAAG 
CTCCAAGAAT GGCTTCCTCA TCTCCACGAT CAAAAAAACA TTGGTCCTGC AGACAGGAGC 
AACTGTGATG ATTATCAGCT CCTTTCAGAA GGTGTGCATC AGGACAAAGA ACTGGGTAGG 
AAATCCTCAA AACCACCTGT AGTAAAAATA AGAGGTGGAT TGGACTTGAA CCAACATGAA 
CTTAGTGCCA TACAAGAGGT AGAATCACCA ACAAGTGGCA GAACTTCTAT ACTAGGTAAA 
CCTGATTTTT ATCAAGACAG AGACCCCCTA AGGGTCTCAA TAAGCCGAGA ACAAAGTTTT 
CTTGGGAGCC CTCTGGACCG TGATCCATTT GGTCATCTTC ACCCAGTTGC TCAGGAGAAC 
ATCTGTGGTG ATGACTCTGA TAAAGCAGTC AAAGTCAAGG AGGCTGTGGT TGAGAATTAT 
GCAGTATTAA GCTATGCTGT GGAGAGAGAA GGCCATACGT ACCTGGATCC AAATGTGAAG 
TTAGATGTTA AGGCTGAAAC ACAAGAAATT TATCATGAGC CACTATCTTC AATAACTGTT 
TCCACTGGGA GTTTTTTAAG TTACGAAAAC ATAGATTTGA GCCTTACAGA GCCAGAGTCA 
TTCCCAGAGC ATGTGGACCA TAGGGAACAA GAACCTCCTG CCGGTAAAGA AGAGGAAACA 
AATGTTTTAA GTTCTAGAGT TCCTTCAACA CAAGATATTT ATCAAAGACA GGACTCTCAG 
GACGTCCATA AACCTCTTTT ATGTGCAGTG GAAACATTGA CATCTGGTCA GACACACATT 
CAACAGATGA GAGACAATTA CATTAATGAA ACAAATTTGA TGACTGAAAA AACAGACTTT 
CGGGTTGACT TTGACTTTCC AGAATTGGAG CACAATTTTC CAAATTTGCA TCGTCAGCTA 
TTTAAACCCT TAGAACCACA TCCAGATTTT GATTTGTTAT CATGTTTCTC TGGGATTTCT 
CAAGACAGTA AAGACTTTAA CCAGAGGTCA GGTTCTTCCT GTGAAAGCCT CCGTGCTACT 
CCATCCCCCA AAAGTATGGC TTCTTTTACA GCACTGAGGA GGGCCAGCCT GCATTCTTCT 
AACACCAGTC TGAACCAGCA GCCTGACCCT AACTTGGCTT GTGACACAGC TCAGAGTTTT 
GCTGTAGAAG ATATTGAGGG GTCTGAACAA TCTTTTCGAC AACTTCGACC AGAATATTCT 
TCACAGGAAG GGAGCCAGCA TGCCGATCTA CCAAGCATTT TTAGCATTGA AGCGAGAGCT 
TCTTCCCAAG ATGTGGAAAA TCAGAACTGT TCCTCTGAAC ATGAACTATT ACAAAACAAG 
CAAAAAAGTG TTCATTTCCA ACTCTCAGTA GGAAATTTGC AAAGTTCAGT CTTCAATTCA 
TCTGGTGAGG CTAATGTATT TCATCAGTTA AATCTACAGC ATGGCACTAC ATGTGGTTCT 
ACCTCTAGTG AGTGGTCAAT AAAAGACCAA CCAGAAGGCA GAAAAGAAAG ACTGGGTTTT 
GAAAAACTAT CAGAAAGACT TGATACAGTG TTACAAGGTC AAGGACTCAC TGATGATAAA 
AAAGAAACCT GTGATTTAAA TCCACACATA GAGGAAACTG ATTCTCATTT ATGTGTAAGG 
ACAGTAGAGA TGGGAACTTC AATTCAGATA CCATATTCAC TCACTGTTCA GAATGAAAAA 
TGTCTTCAGG ACTCAACTAT GGCAGGAATT CCAACAATCG TAGGAAACCC ATCTCAACTT 
GCACAATCAG AGCTCTTTGT AAATTCTGGA TCATTTTCAT TACAGAGATC TATTCCAGTC 
TGGGAAACAG AATCTGGCCA TGGTATAATG GAAGAACCAG AACTTACATT GACAAGCACC 
AGTGATATCA GTATTGCTGA AATGGATTTT GCAAACTTAA CCCTAGAAGA AAAGAGAGAA 
AATGAGGCAA AGAGCTGTTT TCAGGTGAGT GAATTTCTGC CTCTTCTGTC AGAAACAGAA 
AACTCAGATT ATCCAGCCAT GTCAGAACAT CCACTGGAGA GACCAACTGT GTGTGGAGAA 
ACCTTTTCAA GGTGTACAGC TGCACCAGAG AGCTTACAAG AAGCATTCAT AAAAAGAAAA 
AAGGCATTTA TAGAGAGATC CTCACAGAGA CAGAAAGAGA TAAAGGACAA AGTTCATGCC 
TCTGGATATT CTCAAACCAA AACAATAACA GAGAAAACTA CAGGTTCCTT TGTGAGTGAC 
CTAAAGGGTA TGAGTAAAGT TAGGGTGTCG CTTCCTGAAG ATAAAAAGAC TGCACAACCC 
CATACGCACG AAAGGGCAGT AGGATTATAC CTTCAATTAG CTGAAATGAG AAAGCAGAAG 
GAAGACAAAG CAAAGCAAGA TGCACATGCC CAAAACAGAG CAAGGACAAA AGAATTTCAT 
AAGGTAAGCA GCATCCCATA CATACCCCCA ACTTTTCATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843249.1
CDS	137..7381
Translation	MHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_024987481.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987481.1

ORF Insert Sequence:

ATGCATATAA AAGCTCGAAA AGAAGCTCTG CTTGTGGAAA AGGAAAGATC TGCCAAAATT 
GCAAGTCTGC CACCCCCTCC TCCAGCTCTC TTTGAGAATG TTGAAGTAAA GAAAACTAAC 
GTGAAGACGA ACAGTTCTAC CTACCATCAT CTTTGTACTT TTGTGAATAG AGAGGTGGAC 
ACAAAGCAGC CAGATCCTCA TTTGGCTGCT GAAGAAGAAG CTAAAAGATT AGAAGAACTA 
CAGAAGCAGG CAGCGCAAGA GAAAATGGAA CAGAGTGAGA AGGCACATGC CCGGGGATTC 
CAAGCAATGA AAAAGATCCA TTTGGCTCAA AATCAAGAGA AACTAATGAA AGAACTCCAG 
CAGCTCCAGC AAGAGGACCT GGCACGCAGG AGACTGATGG TAGCACGGAT GCCGCCACAA 
CTAGTGGAAC TTCCATACAA ACGCAGTGAA ATGAAAGAAG ATTGGCAGAG AGAGCTAGAA 
TTTGCCTTTG AAGACATGTA CAATGCAGAC AGAAAGGTGA AAGGAAACCT GATTTTGCAC 
CTTGAACCAG AGCCTCTGCC TGCTGTGATT GATCAGATCC AAGACGAAGA ACTGGACCTC 
TCAATGGAAC ATGAAAGTTT AGGGGAGACT GAAAACATTC CAATGACAGA AGCTGAAACA 
GTACATTCTA GTGAAGCAGA CGTTCCCTCA GCAGTGAAGA CCCACCAGAT TCCTTCAAAA 
ATTCTTTTTA AAAAATTATT AAATAAGATC CGAAGCCAAA AATCTCTCTG GACAATTAAA 
TCCGTGTCTG AAGAGGAGGG TGAAATGATT ACAACTGTCA GTGAAGCTGA AAGTAAAGCC 
CTGACAGTTG AATCAGGAGC AACTGTGAGT GAAGACAGAA CATTATCCTC TGGGCAGGAA 
CAAGTTGTTG AAAGTGACAT GCTAACAATT GACTCTGGAC CACTTACTAG TGAAGATAAA 
CCACTTTCAT TTAGTACTGA CTCTGAAAAG GAACAAGAAA TGAAAACCAC TCAGCCTCTC 
ATAGCTCCAA GTTCAATTCT ACTTCATCCT CAGGAAGAAG CAGTCAGAGT TAGAATGGCA 
GCGAGGCAGA AACAGATAAT GGAAATAGAA GAACAGAAGC AAAAGCAGTT GGAATTGCTT 
GAACAGATTG AACAACAGAA GTTAAGATTA GAAACTGATT GCTTCAGAGC TCAGCTGGAA 
GAAGAAAAAA GAAAAAAAAC TCAACAGACT GGGGTCAGCA CTGCTCCAAC ATCATACACC 
ATATGTTCTG ATGAAGATAG GCACAGGCAG ATGATTCATA TTTATCAGCA ACAGCTATTA 
CAGCAAAACA GGTTTCACAA GCAGTCTGTT GAAACAGCCA GGAAGCGATT ACTTGAATAT 
CAGACAATGT TAAGAGGAAA GTACCCATCC ATGTCAGCTA CGCCAGTGAT ACCTGATTCT 
GTTACATCAG TGCCACCACC AAAATCTGAA AGACCCAGCG TTCCATCAGA ACATGAGGAT 
CAAGGTCAGA GACCCAAACT GAGTCCTGAC AAACAACCTG TACAGATCTC CAAATTAGAG 
CAAGATTCTC AGGTTTCAAG ACAGAGTCGC TTTCCACAAA GACAGGTAGA AACAACTGAA 
GCATTAGTCA CTTCAGATGT TTTAGCCAAG CAAGCTTTGG AATCAGAAGA ACACCAAAAG 
CAACTCTCAC AGACTGAAGT ACAACAGAGA GACTACAAAT TGGTCGCTAA AGATTCTCAA 
ATGCTTTCAA GGGCTTTATC ACATGATAAG CCACTGACAG TACAGGATGC TAGAGAAAGA 
GCTGAAACAT CTGGGGCAAC AGCTTTTCAA AGTTTAGACT CCCAGCAAGT GTTCTCAGAG 
AACAATGAAA GTGTATCTTC TAAGTTAATT GAACCATCTT CATTCTTACC ATTGGTAGCT 
GAGCATTCTT TTAGTTCTCT GCCTACTAAA GTCGAGTCTG GAGAAATCCA GCAGCCCTCT 
TCAACTGCGA GCAAAGGTGT AGTTTCTGTA AGCCACTCTG TAGCTGGCCA AATGCAGGAT 
AAGTCTTTGC CATTCTCGGA AAATATCATA GCCCAGCAAA GTAATTTGAA GGCTCTCCAA 
GAACAGTTAG ACCTACAGAA GGAAGTTCTT CGGACAAGAC AGGAAGCTCA GGAACAATTG 
CTTTTGTGTA AACAGAAAGA GTTGGAAGGG CAAACTGGCC TTTCTATATT CCTTCCTTTA 
GTACCTCTGA ATTCATTTGC TTCACTGCCT TCAGCCCAAG CTGAGTCAAG GAGAATCCAG 
GAATCTTCTC CAACCAATAA AGAGACTGGT GTTTCCTCAG GCCATCCTGG GGTCCCACAA 
CTTCAGGATA GGCTTAATTT AAAATTTTTC CAAGAACAGT TAAAAACGCA GAGGGACAGC 
CTTCAGGCTA GGCGGGAAGC CCAGGAAGTA TTATGTGTGC ATAAACAGAA TGAGTTGGAT 
GGGAGAGTAT GGGCTGAACA GACTGGGTCC TCTTCTCTCC CACCTCAAGT AGCTCAGCAT 
ACATTTACTT CACTGCCTTC TGCTGGTCCT CAGTCTGGAA AAATCCAGGA CCAGTATTCA 
TCTAAGACTG AGAAGGAGCA TTTCTCAATC CAGACTGAAA TGCCCACATC TCAGGATGGG 
TCTTTAGGTT TCCCACAACA GTTCCAACCT TTGCACGATT GTTTGAAGTT GCTCCAAGAG 
CAGCTGATTA CACAGAGGGA TGCTCTCCAG GCCAGGCATG AAGCCCAAGT GGAATTACTT 
TCACGTAAAC AAAGGGATTT GGAAGACGCT AAGTCTGTGC AGATGAGTTC TTCGTTCCTG 
CCAGTGGTCA CTCAACATTC ACTTGCTTCA CAAGTTTCAG CTAAAACTGA GCCTAGGAGA 
ATTCAGAACT TTTATTTCTC TGAGAGGGAT AGTGTTATTC CCTCAAATAA TTTGGTAATG 
CCAGCATCTC AGGATGCGTC TCTTAGTTTT CCACAGTATA GTCTGCCACG GCAGGAAAAT 
TTAACAGCAC TCCACAATCA GTCACACGTT CAGAGGGTAA TACTTGGCGA TAAACAAGAA 
ACTCAGGAAT TTGTACACAA ACAAAGTGAA TTAGAAAAAA TTTTCCCTTC TGAACAGACT 
GGCACTTCTT TGTCTCAGGG AGCTGAATCT GAAAGATGCC AGGAATACAT GTCATTCAGG 
AGTGACAGTA CTGTTCCCTT GAGCCACTGG AAGATCCCAA GATCTCAGGA AAGACTTCTG 
GGATTTTCAC GACATACACA ACCTCTGCAA GGTAATTTGG AAGGACAGCA AGAACAGTTA 
GACAGAGAAG AGGAGGCCCT TCAGTTCAGC CAGAGATCCC AAGGGAATGT ATCTTCTGGA 
CAGATTTGCC CCTCGTTCAC ACCCCGGTTA GGGCAGCTCT CTCTTACTTC ATTGCCTTCT 
GCTGAATCTC GGGAACCTCT TTCAGCAGAG AGTGAGAGTA GAACTCCTTC AAGCCGTTTT 
CACATCCCGG TGTTGCAGGA TAGACTTTGG AAGTTATCAC AGCTTATACA GCCTCAGCAA 
GATAACCTGA AGTCACTCCA AGAACAGTTA GCTACACAGA GGGAAGCCAT CATTCAGTCT 
AGGCAGGAAG CTCAGCAAGA ATTTCTTTTG CACAAACAGG GTGAGTGGAA GGGAAGAGTG 
TCTCCAGAGC AGGTTGGCAC CTCCTCCTTC CTACCCCAAG TCATACAGCC TCTTGCTCCA 
TTACCTCCTA GTAAAGCTGG TAGAATCCAA GAACCTTGTT CAACTAAGAG TGATAATATA 
GTCTCCTCAG GTCATTCTGA GATACCAAGG TTGCCTGATA GACTTTTAGG TTTACCACAG 
CCTGTTTTGC CTCAACAAGA TTATCCAGTC ACATTTCAAC AAGAACATTT GTATGCCCAG 
ACAAACCCCC TTCCATGTAG CGAGAAAATC CAAAGAGAGT TGGTTTTGCC CAGACAATAT 
ACACTTGAGG GAAAGTCACC TAAGCATTTT GTCCAACCTC ACCATGGTGA TTTAAAGGCC 
TATCAACGGC AGTTAGATAT ACAGAGGGAA GTCCAAGAAG AATTCCTTTT GCAAACACTA 
AGTAAATTGG AAAAAGGGGT CTCAGCTGAG CAGACCACCA CCTCTTCATC CTTATCCCAC 
GTAGCACTGC CTGTTGCTGA CTCTAAAGGA ATGCCAAAGT GTTTTCCAGC CAGAAGTGAC 
CTTGCTGTTT CCTCAAGTCA TCCTGAGATT CTTAGACCTC AGGAGAGGCC TTTGCATTTA 
TCCCAGGCTG TTTTGCCTCA GCAAGACCAT GTGTCCACAC AGTTGGGCCT TCAGGGTGAA 
GTGCTACGTT TTAGTGAAAA GGCCCAGGAA GAACTGCTAA GAGGCAATCA CACAAAACTG 
ATTGAAGGTG ATGCTTCTGG GCAGCCTGTG CCCTCTTTGT TTCTACCCAA GGAAAGAGAA 
CATTCGTTTA TTCCACTACC TTTTGCTGAG GTAAAATCTC AAAACATACG TGAATTGTAT 
TCAGCCAAGA TTGAATGTGC AGCTCCCTCT AGTGCTTCTG TGAGCCCAAG ACTTCAAGAT 
AGAATTTTGA GTTTTTCACA ACCCATCTTT GCTCAGCAAG ATAACTGGGG ACTTCAGAAG 
CAGTTGGAGC TACAAAAAGA AGTTCTGCCT TTTAGTCAGA AAGCCCAAGA AGTATTGCTT 
GTACCAACAC AGACAGCCTT GCAGCAACAG ACACACAAAC ATCGGGAGAC TCTGAAGGAT 
TTCTTCAAAG ACAGCCAGGA AAGTAAGCCC ACAGTTGAAA ATGATTTTGA AACCCAGAAG 
CTCCAAGAAT GGCTTCCTCA TCTCCACGAT CAAAAAAACA TTGGTCCTGC AGACAGGAGC 
AACTGTGATG ATTATCAGCT CCTTTCAGAA GGTGTGCATC AGGACAAAGA ACTGGGTAGG 
AAATCCTCAA AACCACCTGT AGTAAAAATA AGAGGTGGAT TGGACTTGAA CCAACATGAA 
CTTAGTGCCA TACAAGAGGT AGAATCACCA ACAAGTGGCA GAACTTCTAT ACTAGGTAAA 
CCTGATTTTT ATCAAGACAG AGACCCCCTA AGGGTCTCAA TAAGCCGAGA ACAAAGTTTT 
CTTGGGAGCC CTCTGGACCG TGATCCATTT GGTCATCTTC ACCCAGTTGC TCAGGAGAAC 
ATCTGTGGTG ATGACTCTGA TAAAGCAGTC AAAGTCAAGG AGGCTGTGGT TGAGAATTAT 
GCAGTATTAA GCTATGCTGT GGAGAGAGAA GGCCATACGT ACCTGGATCC AAATGTGAAG 
TTAGATGTTA AGGCTGAAAC ACAAGAAATT TATCATGAGC CACTATCTTC AATAACTGTT 
TCCACTGGGA GTTTTTTAAG TTACGAAAAC ATAGATTTGA GCCTTACAGA GCCAGAGTCA 
TTCCCAGAGC ATGTGGACCA TAGGGAACAA GAACCTCCTG CCGGTAAAGA AGAGGAAACA 
AATGTTTTAA GTTCTAGAGT TCCTTCAACA CAAGATATTT ATCAAAGACA GGACTCTCAG 
GACGTCCATA AACCTCTTTT ATGTGCAGTG GAAACATTGA CATCTGGTCA GACACACATT 
CAACAGATGA GAGACAATTA CATTAATGAA ACAAATTTGA TGACTGAAAA AACAGACTTT 
CGGGTTGACT TTGACTTTCC AGAATTGGAG CACAATTTTC CAAATTTGCA TCGTCAGCTA 
TTTAAACCCT TAGAACCACA TCCAGATTTT GATTTGTTAT CATGTTTCTC TGGGATTTCT 
CAAGACAGTA AAGACTTTAA CCAGAGGTCA GGTTCTTCCT GTGAAAGCCT CCGTGCTACT 
CCATCCCCCA AAAGTATGGC TTCTTTTACA GCACTGAGGA GGGCCAGCCT GCATTCTTCT 
AACACCAGTC TGAACCAGCA GCCTGACCCT AACTTGGCTT GTGACACAGC TCAGAGTTTT 
GCTGTAGAAG ATATTGAGGG GTCTGAACAA TCTTTTCGAC AACTTCGACC AGAATATTCT 
TCACAGGAAG GGAGCCAGCA TGCCGATCTA CCAAGCATTT TTAGCATTGA AGCGAGAGCT 
TCTTCCCAAG ATGTGGAAAA TCAGAACTGT TCCTCTGAAC ATGAACTATT ACAAAACAAG 
CAAAAAAGTG TTCATTTCCA ACTCTCAGTA GGAAATTTGC AAAGTTCAGT CTTCAATTCA 
TCTGGTGAGG CTAATGTATT TCATCAGTTA AATCTACAGC ATGGCACTAC ATGTGGTTCT 
ACCTCTAGTG AGTGGTCAAT AAAAGACCAA CCAGAAGGCA GAAAAGAAAG ACTGGGTTTT 
GAAAAACTAT CAGAAAGACT TGATACAGTG TTACAAGGTC AAGGACTCAC TGATGATAAA 
AAAGAAACCT GTGATTTAAA TCCACACATA GAGGAAACTG ATTCTCATTT ATGTGTAAGG 
ACAGTAGAGA TGGGAACTTC AATTCAGATA CCATATTCAC TCACTGTTCA GAATGAAAAA 
TGTCTTCAGG ACTCAACTAT GGCAGGAATT CCAACAATCG TAGGAAACCC ATCTCAACTT 
GCACAATCAG AGCTCTTTGT AAATTCTGGA TCATTTTCAT TACAGAGATC TATTCCAGTC 
TGGGAAACAG AATCTGGCCA TGGTATAATG GAAGAACCAG AACTTACATT GACAAGCACC 
AGTGATATCA GTATTGCTGA AATGGATTTT GCAAACTTAA CCCTAGAAGA AAAGAGAGAA 
AATGAGGCAA AGAGCTGTTT TCAGGTGAGT GAATTTCTGC CTCTTCTGTC AGAAACAGAA 
AACTCAGATT ATCCAGCCAT GTCAGAACAT CCACTGGAGA GACCAACTGT GTGTGGAGAA 
ACCTTTTCAA GGTGTACAGC TGCACCAGAG AGCTTACAAG AAGCATTCAT AAAAAGAAAA 
AAGGCATTTA TAGAGAGATC CTCACAGAGA CAGAAAGAGA TAAAGGACAA AGTTCATGCC 
TCTGGATATT CTCAAACCAA AACAATAACA GAGAAAACTA CAGGTTCCTT TGTGAGTGAC 
CTAAAGGGTA TGAGTAAAGT TAGGGTGTCG CTTCCTGAAG ATAAAAAGAC TGCACAACCC 
CATACGCACG AAAGGGCAGT AGGATTATAC CTTCAATTAG CTGAAATGAG AAAGCAGAAG 
GAAGACAAAG CAAAGCAAGA TGCACATGCC CAAAACAGAG CAAGGACAAA AGAATTTCAT 
AAGGTAAGCA GCATCCCATA CATACCCCCA ACTTTTCATG TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820789.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819091.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.

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CloneID	OBa52314
Clone ID Related Accession (Same CDS sequence)	XM_010820789.2 , XM_015469901.1 , XM_010820789.3
Accession Version	XM_010820789.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819091.1
CDS	162..7142
Translation	MEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820789.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820789.2

ORF Insert Sequence:

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_015469901.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325387.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.

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CloneID	OBa52314
Clone ID Related Accession (Same CDS sequence)	XM_010820789.2 , XM_015469901.1 , XM_010820789.3
Accession Version	XM_015469901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325387.1
CDS	162..7142
Translation	MEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_015469901.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469901.1

ORF Insert Sequence:

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820789.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819091.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X7, mRNA.

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CloneID	OBa52314
Clone ID Related Accession (Same CDS sequence)	XM_010820789.2 , XM_015469901.1 , XM_010820789.3
Accession Version	XM_010820789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819091.1
CDS	855..7835
Translation	MEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820789.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820789.3

ORF Insert Sequence:

ATGGAACAGA GTGAGAAGGC ACATGCCCGG GGATTCCAAG CAATGAAAAA GATCCATTTG 
GCTCAAAATC AAGAGAAACT AATGAAAGAA CTCCAGCAGC TCCAGCAAGA GGACCTGGCA 
CGCAGGAGAC TGATGGTAGC ACGGATGCCG CCACAACTAG TGGAACTTCC ATACAAACGC 
AGTGAAATGA AAGAAGATTG GCAGAGAGAG CTAGAATTTG CCTTTGAAGA CATGTACAAT 
GCAGACAGAA AGGTGAAAGG AAACCTGATT TTGCACCTTG AACCAGAGCC TCTGCCTGCT 
GTGATTGATC AGATCCAAGA CGAAGAACTG GACCTCTCAA TGGAACATGA AAGTTTAGGG 
GAGACTGAAA ACATTCCAAT GACAGAAGCT GAAACAGTAC ATTCTAGTGA AGCAGACGTT 
CCCTCAGCAG TGAAGACCCA CCAGATTCCT TCAAAAATTC TTTTTAAAAA ATTATTAAAT 
AAGATCCGAA GCCAAAAATC TCTCTGGACA ATTAAATCCG TGTCTGAAGA GGAGGGTGAA 
ATGATTACAA CTGTCAGTGA AGCTGAAAGT AAAGCCCTGA CAGTTGAATC AGGAGCAACT 
GTGAGTGAAG ACAGAACATT ATCCTCTGGG CAGGAACAAG TTGTTGAAAG TGACATGCTA 
ACAATTGACT CTGGACCACT TACTAGTGAA GATAAACCAC TTTCATTTAG TACTGACTCT 
GAAAAGGAAC AAGAAATGAA AACCACTCAG CCTCTCATAG CTCCAAGTTC AATTCTACTT 
CATCCTCAGG AAGAAGCAGT CAGAGTTAGA ATGGCAGCGA GGCAGAAACA GATAATGGAA 
ATAGAAGAAC AGAAGCAAAA GCAGTTGGAA TTGCTTGAAC AGATTGAACA ACAGAAGTTA 
AGATTAGAAA CTGATTGCTT CAGAGCTCAG CTGGAAGAAG AAAAAAGAAA AAAAACTCAA 
CAGACTGGGG TCAGCACTGC TCCAACATCA TACACCATAT GTTCTGATGA AGATAGGCAC 
AGGCAGATGA TTCATATTTA TCAGCAACAG CTATTACAGC AAAACAGGTT TCACAAGCAG 
TCTGTTGAAA CAGCCAGGAA GCGATTACTT GAATATCAGA CAATGTTAAG AGGAAAGTAC 
CCATCCATGT CAGCTACGCC AGTGATACCT GATTCTGTTA CATCAGTGCC ACCACCAAAA 
TCTGAAAGAC CCAGCGTTCC ATCAGAACAT GAGGATCAAG GTCAGAGACC CAAACTGAGT 
CCTGACAAAC AACCTGTACA GATCTCCAAA TTAGAGCAAG ATTCTCAGGT TTCAAGACAG 
AGTCGCTTTC CACAAAGACA GGTAGAAACA ACTGAAGCAT TAGTCACTTC AGATGTTTTA 
GCCAAGCAAG CTTTGGAATC AGAAGAACAC CAAAAGCAAC TCTCACAGAC TGAAGTACAA 
CAGAGAGACT ACAAATTGGT CGCTAAAGAT TCTCAAATGC TTTCAAGGGC TTTATCACAT 
GATAAGCCAC TGACAGTACA GGATGCTAGA GAAAGAGCTG AAACATCTGG GGCAACAGCT 
TTTCAAAGTT TAGACTCCCA GCAAGTGTTC TCAGAGAACA ATGAAAGTGT ATCTTCTAAG 
TTAATTGAAC CATCTTCATT CTTACCATTG GTAGCTGAGC ATTCTTTTAG TTCTCTGCCT 
ACTAAAGTCG AGTCTGGAGA AATCCAGCAG CCCTCTTCAA CTGCGAGCAA AGGTGTAGTT 
TCTGTAAGCC ACTCTGTAGC TGGCCAAATG CAGGATAAGT CTTTGCCATT CTCGGAAAAT 
ATCATAGCCC AGCAAAGTAA TTTGAAGGCT CTCCAAGAAC AGTTAGACCT ACAGAAGGAA 
GTTCTTCGGA CAAGACAGGA AGCTCAGGAA CAATTGCTTT TGTGTAAACA GAAAGAGTTG 
GAAGGGCAAA CTGGCCTTTC TATATTCCTT CCTTTAGTAC CTCTGAATTC ATTTGCTTCA 
CTGCCTTCAG CCCAAGCTGA GTCAAGGAGA ATCCAGGAAT CTTCTCCAAC CAATAAAGAG 
ACTGGTGTTT CCTCAGGCCA TCCTGGGGTC CCACAACTTC AGGATAGGCT TAATTTAAAA 
TTTTTCCAAG AACAGTTAAA AACGCAGAGG GACAGCCTTC AGGCTAGGCG GGAAGCCCAG 
GAAGTATTAT GTGTGCATAA ACAGAATGAG TTGGATGGGA GAGTATGGGC TGAACAGACT 
GGGTCCTCTT CTCTCCCACC TCAAGTAGCT CAGCATACAT TTACTTCACT GCCTTCTGCT 
GGTCCTCAGT CTGGAAAAAT CCAGGACCAG TATTCATCTA AGACTGAGAA GGAGCATTTC 
TCAATCCAGA CTGAAATGCC CACATCTCAG GATGGGTCTT TAGGTTTCCC ACAACAGTTC 
CAACCTTTGC ACGATTGTTT GAAGTTGCTC CAAGAGCAGC TGATTACACA GAGGGATGCT 
CTCCAGGCCA GGCATGAAGC CCAAGTGGAA TTACTTTCAC GTAAACAAAG GGATTTGGAA 
GACGCTAAGT CTGTGCAGAT GAGTTCTTCG TTCCTGCCAG TGGTCACTCA ACATTCACTT 
GCTTCACAAG TTTCAGCTAA AACTGAGCCT AGGAGAATTC AGAACTTTTA TTTCTCTGAG 
AGGGATAGTG TTATTCCCTC AAATAATTTG GTAATGCCAG CATCTCAGGA TGCGTCTCTT 
AGTTTTCCAC AGTATAGTCT GCCACGGCAG GAAAATTTAA CAGCACTCCA CAATCAGTCA 
CACGTTCAGA GGGTAATACT TGGCGATAAA CAAGAAACTC AGGAATTTGT ACACAAACAA 
AGTGAATTAG AAAAAATTTT CCCTTCTGAA CAGACTGGCA CTTCTTTGTC TCAGGGAGCT 
GAATCTGAAA GATGCCAGGA ATACATGTCA TTCAGGAGTG ACAGTACTGT TCCCTTGAGC 
CACTGGAAGA TCCCAAGATC TCAGGAAAGA CTTCTGGGAT TTTCACGACA TACACAACCT 
CTGCAAGGTA ATTTGGAAGG ACAGCAAGAA CAGTTAGACA GAGAAGAGGA GGCCCTTCAG 
TTCAGCCAGA GATCCCAAGG GAATGTATCT TCTGGACAGA TTTGCCCCTC GTTCACACCC 
CGGTTAGGGC AGCTCTCTCT TACTTCATTG CCTTCTGCTG AATCTCGGGA ACCTCTTTCA 
GCAGAGAGTG AGAGTAGAAC TCCTTCAAGC CGTTTTCACA TCCCGGTGTT GCAGGATAGA 
CTTTGGAAGT TATCACAGCT TATACAGCCT CAGCAAGATA ACCTGAAGTC ACTCCAAGAA 
CAGTTAGCTA CACAGAGGGA AGCCATCATT CAGTCTAGGC AGGAAGCTCA GCAAGAATTT 
CTTTTGCACA AACAGGGTGA GTGGAAGGGA AGAGTGTCTC CAGAGCAGGT TGGCACCTCC 
TCCTTCCTAC CCCAAGTCAT ACAGCCTCTT GCTCCATTAC CTCCTAGTAA AGCTGGTAGA 
ATCCAAGAAC CTTGTTCAAC TAAGAGTGAT AATATAGTCT CCTCAGGTCA TTCTGAGATA 
CCAAGGTTGC CTGATAGACT TTTAGGTTTA CCACAGCCTG TTTTGCCTCA ACAAGATTAT 
CCAGTCACAT TTCAACAAGA ACATTTGTAT GCCCAGACAA ACCCCCTTCC ATGTAGCGAG 
AAAATCCAAA GAGAGTTGGT TTTGCCCAGA CAATATACAC TTGAGGGAAA GTCACCTAAG 
CATTTTGTCC AACCTCACCA TGGTGATTTA AAGGCCTATC AACGGCAGTT AGATATACAG 
AGGGAAGTCC AAGAAGAATT CCTTTTGCAA ACACTAAGTA AATTGGAAAA AGGGGTCTCA 
GCTGAGCAGA CCACCACCTC TTCATCCTTA TCCCACGTAG CACTGCCTGT TGCTGACTCT 
AAAGGAATGC CAAAGTGTTT TCCAGCCAGA AGTGACCTTG CTGTTTCCTC AAGTCATCCT 
GAGATTCTTA GACCTCAGGA GAGGCCTTTG CATTTATCCC AGGCTGTTTT GCCTCAGCAA 
GACCATGTGT CCACACAGTT GGGCCTTCAG GGTGAAGTGC TACGTTTTAG TGAAAAGGCC 
CAGGAAGAAC TGCTAAGAGG CAATCACACA AAACTGATTG AAGGTGATGC TTCTGGGCAG 
CCTGTGCCCT CTTTGTTTCT ACCCAAGGAA AGAGAACATT CGTTTATTCC ACTACCTTTT 
GCTGAGGTAA AATCTCAAAA CATACGTGAA TTGTATTCAG CCAAGATTGA ATGTGCAGCT 
CCCTCTAGTG CTTCTGTGAG CCCAAGACTT CAAGATAGAA TTTTGAGTTT TTCACAACCC 
ATCTTTGCTC AGCAAGATAA CTGGGGACTT CAGAAGCAGT TGGAGCTACA AAAAGAAGTT 
CTGCCTTTTA GTCAGAAAGC CCAAGAAGTA TTGCTTGTAC CAACACAGAC AGCCTTGCAG 
CAACAGACAC ACAAACATCG GGAGACTCTG AAGGATTTCT TCAAAGACAG CCAGGAAAGT 
AAGCCCACAG TTGAAAATGA TTTTGAAACC CAGAAGCTCC AAGAATGGCT TCCTCATCTC 
CACGATCAAA AAAACATTGG TCCTGCAGAC AGGAGCAACT GTGATGATTA TCAGCTCCTT 
TCAGAAGGTG TGCATCAGGA CAAAGAACTG GGTAGGAAAT CCTCAAAACC ACCTGTAGTA 
AAAATAAGAG GTGGATTGGA CTTGAACCAA CATGAACTTA GTGCCATACA AGAGGTAGAA 
TCACCAACAA GTGGCAGAAC TTCTATACTA GGTAAACCTG ATTTTTATCA AGACAGAGAC 
CCCCTAAGGG TCTCAATAAG CCGAGAACAA AGTTTTCTTG GGAGCCCTCT GGACCGTGAT 
CCATTTGGTC ATCTTCACCC AGTTGCTCAG GAGAACATCT GTGGTGATGA CTCTGATAAA 
GCAGTCAAAG TCAAGGAGGC TGTGGTTGAG AATTATGCAG TATTAAGCTA TGCTGTGGAG 
AGAGAAGGCC ATACGTACCT GGATCCAAAT GTGAAGTTAG ATGTTAAGGC TGAAACACAA 
GAAATTTATC ATGAGCCACT ATCTTCAATA ACTGTTTCCA CTGGGAGTTT TTTAAGTTAC 
GAAAACATAG ATTTGAGCCT TACAGAGCCA GAGTCATTCC CAGAGCATGT GGACCATAGG 
GAACAAGAAC CTCCTGCCGG TAAAGAAGAG GAAACAAATG TTTTAAGTTC TAGAGTTCCT 
TCAACACAAG ATATTTATCA AAGACAGGAC TCTCAGGACG TCCATAAACC TCTTTTATGT 
GCAGTGGAAA CATTGACATC TGGTCAGACA CACATTCAAC AGATGAGAGA CAATTACATT 
AATGAAACAA ATTTGATGAC TGAAAAAACA GACTTTCGGG TTGACTTTGA CTTTCCAGAA 
TTGGAGCACA ATTTTCCAAA TTTGCATCGT CAGCTATTTA AACCCTTAGA ACCACATCCA 
GATTTTGATT TGTTATCATG TTTCTCTGGG ATTTCTCAAG ACAGTAAAGA CTTTAACCAG 
AGGTCAGGTT CTTCCTGTGA AAGCCTCCGT GCTACTCCAT CCCCCAAAAG TATGGCTTCT 
TTTACAGCAC TGAGGAGGGC CAGCCTGCAT TCTTCTAACA CCAGTCTGAA CCAGCAGCCT 
GACCCTAACT TGGCTTGTGA CACAGCTCAG AGTTTTGCTG TAGAAGATAT TGAGGGGTCT 
GAACAATCTT TTCGACAACT TCGACCAGAA TATTCTTCAC AGGAAGGGAG CCAGCATGCC 
GATCTACCAA GCATTTTTAG CATTGAAGCG AGAGCTTCTT CCCAAGATGT GGAAAATCAG 
AACTGTTCCT CTGAACATGA ACTATTACAA AACAAGCAAA AAAGTGTTCA TTTCCAACTC 
TCAGTAGGAA ATTTGCAAAG TTCAGTCTTC AATTCATCTG GTGAGGCTAA TGTATTTCAT 
CAGTTAAATC TACAGCATGG CACTACATGT GGTTCTACCT CTAGTGAGTG GTCAATAAAA 
GACCAACCAG AAGGCAGAAA AGAAAGACTG GGTTTTGAAA AACTATCAGA AAGACTTGAT 
ACAGTGTTAC AAGGTCAAGG ACTCACTGAT GATAAAAAAG AAACCTGTGA TTTAAATCCA 
CACATAGAGG AAACTGATTC TCATTTATGT GTAAGGACAG TAGAGATGGG AACTTCAATT 
CAGATACCAT ATTCACTCAC TGTTCAGAAT GAAAAATGTC TTCAGGACTC AACTATGGCA 
GGAATTCCAA CAATCGTAGG AAACCCATCT CAACTTGCAC AATCAGAGCT CTTTGTAAAT 
TCTGGATCAT TTTCATTACA GAGATCTATT CCAGTCTGGG AAACAGAATC TGGCCATGGT 
ATAATGGAAG AACCAGAACT TACATTGACA AGCACCAGTG ATATCAGTAT TGCTGAAATG 
GATTTTGCAA ACTTAACCCT AGAAGAAAAG AGAGAAAATG AGGCAAAGAG CTGTTTTCAG 
GTGAGTGAAT TTCTGCCTCT TCTGTCAGAA ACAGAAAACT CAGATTATCC AGCCATGTCA 
GAACATCCAC TGGAGAGACC AACTGTGTGT GGAGAAACCT TTTCAAGGTG TACAGCTGCA 
CCAGAGAGCT TACAAGAAGC ATTCATAAAA AGAAAAAAGG CATTTATAGA GAGATCCTCA 
CAGAGACAGA AAGAGATAAA GGACAAAGTT CATGCCTCTG GATATTCTCA AACCAAAACA 
ATAACAGAGA AAACTACAGG TTCCTTTGTG AGTGACCTAA AGGGTATGAG TAAAGTTAGG 
GTGTCGCTTC CTGAAGATAA AAAGACTGCA CAACCCCATA CGCACGAAAG GGCAGTAGGA 
TTATACCTTC AATTAGCTGA AATGAGAAAG CAGAAGGAAG ACAAAGCAAA GCAAGATGCA 
CATGCCCAAA ACAGAGCAAG GACAAAAGAA TTTCATAAGG TAAGCAGCAT CCCATACATA 
CCCCCAACTT TTCATGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_015469902.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325388.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.

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CloneID	OBa52315
Clone ID Related Accession (Same CDS sequence)	XM_015469902.1 , XM_010820790.3 , XM_010820790.2
Accession Version	XM_015469902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325388.1
CDS	97..7062
Translation	MLFQVFRLAFTSSFQIQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_015469902.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469902.1

ORF Insert Sequence:

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820790.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819092.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X8, mRNA.

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CloneID	OBa52315
Clone ID Related Accession (Same CDS sequence)	XM_015469902.1 , XM_010820790.3 , XM_010820790.2
Accession Version	XM_010820790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819092.1
CDS	104..7069
Translation	MLFQVFRLAFTSSFQIQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820790.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820790.3

ORF Insert Sequence:

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820790.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819092.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.

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CloneID	OBa52315
Clone ID Related Accession (Same CDS sequence)	XM_015469902.1 , XM_010820790.3 , XM_010820790.2
Accession Version	XM_010820790.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820790.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819092.1
CDS	97..7062
Translation	MLFQVFRLAFTSSFQIQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQRSGSSCESLRATPSPKSMASFTALRRASLHSSNTSLNQQPDPNLACDTAQSFAVEDIEGSEQSFRQLRPEYSSQEGSQHADLPSIFSIEARASSQDVENQNCSSEHELLQNKQKSVHFQLSVGNLQSSVFNSSGEANVFHQLNLQHGTTCGSTSSEWSIKDQPEGRKERLGFEKLSERLDTVLQGQGLTDDKKETCDLNPHIEETDSHLCVRTVEMGTSIQIPYSLTVQNEKCLQDSTMAGIPTIVGNPSQLAQSELFVNSGSFSLQRSIPVWETESGHGIMEEPELTLTSTSDISIAEMDFANLTLEEKRENEAKSCFQVSEFLPLLSETENSDYPAMSEHPLERPTVCGETFSRCTAAPESLQEAFIKRKKAFIERSSQRQKEIKDKVHASGYSQTKTITEKTTGSFVSDLKGMSKVRVSLPEDKKTAQPHTHERAVGLYLQLAEMRKQKEDKAKQDAHAQNRARTKEFHKVSSIPYIPPTFHV

Target ORF information:

RefSeq Version	XM_010820790.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820790.2

ORF Insert Sequence:

ATGTTATTCC AAGTTTTCCG ACTTGCTTTT ACCTCCTCGT TTCAAATTCA GAATCAAGAG 
AAACTAATGA AAGAACTCCA GCAGCTCCAG CAAGAGGACC TGGCACGCAG GAGACTGATG 
GTAGCACGGA TGCCGCCACA ACTAGTGGAA CTTCCATACA AACGCAGTGA AATGAAAGAA 
GATTGGCAGA GAGAGCTAGA ATTTGCCTTT GAAGACATGT ACAATGCAGA CAGAAAGGTG 
AAAGGAAACC TGATTTTGCA CCTTGAACCA GAGCCTCTGC CTGCTGTGAT TGATCAGATC 
CAAGACGAAG AACTGGACCT CTCAATGGAA CATGAAAGTT TAGGGGAGAC TGAAAACATT 
CCAATGACAG AAGCTGAAAC AGTACATTCT AGTGAAGCAG ACGTTCCCTC AGCAGTGAAG 
ACCCACCAGA TTCCTTCAAA AATTCTTTTT AAAAAATTAT TAAATAAGAT CCGAAGCCAA 
AAATCTCTCT GGACAATTAA ATCCGTGTCT GAAGAGGAGG GTGAAATGAT TACAACTGTC 
AGTGAAGCTG AAAGTAAAGC CCTGACAGTT GAATCAGGAG CAACTGTGAG TGAAGACAGA 
ACATTATCCT CTGGGCAGGA ACAAGTTGTT GAAAGTGACA TGCTAACAAT TGACTCTGGA 
CCACTTACTA GTGAAGATAA ACCACTTTCA TTTAGTACTG ACTCTGAAAA GGAACAAGAA 
ATGAAAACCA CTCAGCCTCT CATAGCTCCA AGTTCAATTC TACTTCATCC TCAGGAAGAA 
GCAGTCAGAG TTAGAATGGC AGCGAGGCAG AAACAGATAA TGGAAATAGA AGAACAGAAG 
CAAAAGCAGT TGGAATTGCT TGAACAGATT GAACAACAGA AGTTAAGATT AGAAACTGAT 
TGCTTCAGAG CTCAGCTGGA AGAAGAAAAA AGAAAAAAAA CTCAACAGAC TGGGGTCAGC 
ACTGCTCCAA CATCATACAC CATATGTTCT GATGAAGATA GGCACAGGCA GATGATTCAT 
ATTTATCAGC AACAGCTATT ACAGCAAAAC AGGTTTCACA AGCAGTCTGT TGAAACAGCC 
AGGAAGCGAT TACTTGAATA TCAGACAATG TTAAGAGGAA AGTACCCATC CATGTCAGCT 
ACGCCAGTGA TACCTGATTC TGTTACATCA GTGCCACCAC CAAAATCTGA AAGACCCAGC 
GTTCCATCAG AACATGAGGA TCAAGGTCAG AGACCCAAAC TGAGTCCTGA CAAACAACCT 
GTACAGATCT CCAAATTAGA GCAAGATTCT CAGGTTTCAA GACAGAGTCG CTTTCCACAA 
AGACAGGTAG AAACAACTGA AGCATTAGTC ACTTCAGATG TTTTAGCCAA GCAAGCTTTG 
GAATCAGAAG AACACCAAAA GCAACTCTCA CAGACTGAAG TACAACAGAG AGACTACAAA 
TTGGTCGCTA AAGATTCTCA AATGCTTTCA AGGGCTTTAT CACATGATAA GCCACTGACA 
GTACAGGATG CTAGAGAAAG AGCTGAAACA TCTGGGGCAA CAGCTTTTCA AAGTTTAGAC 
TCCCAGCAAG TGTTCTCAGA GAACAATGAA AGTGTATCTT CTAAGTTAAT TGAACCATCT 
TCATTCTTAC CATTGGTAGC TGAGCATTCT TTTAGTTCTC TGCCTACTAA AGTCGAGTCT 
GGAGAAATCC AGCAGCCCTC TTCAACTGCG AGCAAAGGTG TAGTTTCTGT AAGCCACTCT 
GTAGCTGGCC AAATGCAGGA TAAGTCTTTG CCATTCTCGG AAAATATCAT AGCCCAGCAA 
AGTAATTTGA AGGCTCTCCA AGAACAGTTA GACCTACAGA AGGAAGTTCT TCGGACAAGA 
CAGGAAGCTC AGGAACAATT GCTTTTGTGT AAACAGAAAG AGTTGGAAGG GCAAACTGGC 
CTTTCTATAT TCCTTCCTTT AGTACCTCTG AATTCATTTG CTTCACTGCC TTCAGCCCAA 
GCTGAGTCAA GGAGAATCCA GGAATCTTCT CCAACCAATA AAGAGACTGG TGTTTCCTCA 
GGCCATCCTG GGGTCCCACA ACTTCAGGAT AGGCTTAATT TAAAATTTTT CCAAGAACAG 
TTAAAAACGC AGAGGGACAG CCTTCAGGCT AGGCGGGAAG CCCAGGAAGT ATTATGTGTG 
CATAAACAGA ATGAGTTGGA TGGGAGAGTA TGGGCTGAAC AGACTGGGTC CTCTTCTCTC 
CCACCTCAAG TAGCTCAGCA TACATTTACT TCACTGCCTT CTGCTGGTCC TCAGTCTGGA 
AAAATCCAGG ACCAGTATTC ATCTAAGACT GAGAAGGAGC ATTTCTCAAT CCAGACTGAA 
ATGCCCACAT CTCAGGATGG GTCTTTAGGT TTCCCACAAC AGTTCCAACC TTTGCACGAT 
TGTTTGAAGT TGCTCCAAGA GCAGCTGATT ACACAGAGGG ATGCTCTCCA GGCCAGGCAT 
GAAGCCCAAG TGGAATTACT TTCACGTAAA CAAAGGGATT TGGAAGACGC TAAGTCTGTG 
CAGATGAGTT CTTCGTTCCT GCCAGTGGTC ACTCAACATT CACTTGCTTC ACAAGTTTCA 
GCTAAAACTG AGCCTAGGAG AATTCAGAAC TTTTATTTCT CTGAGAGGGA TAGTGTTATT 
CCCTCAAATA ATTTGGTAAT GCCAGCATCT CAGGATGCGT CTCTTAGTTT TCCACAGTAT 
AGTCTGCCAC GGCAGGAAAA TTTAACAGCA CTCCACAATC AGTCACACGT TCAGAGGGTA 
ATACTTGGCG ATAAACAAGA AACTCAGGAA TTTGTACACA AACAAAGTGA ATTAGAAAAA 
ATTTTCCCTT CTGAACAGAC TGGCACTTCT TTGTCTCAGG GAGCTGAATC TGAAAGATGC 
CAGGAATACA TGTCATTCAG GAGTGACAGT ACTGTTCCCT TGAGCCACTG GAAGATCCCA 
AGATCTCAGG AAAGACTTCT GGGATTTTCA CGACATACAC AACCTCTGCA AGGTAATTTG 
GAAGGACAGC AAGAACAGTT AGACAGAGAA GAGGAGGCCC TTCAGTTCAG CCAGAGATCC 
CAAGGGAATG TATCTTCTGG ACAGATTTGC CCCTCGTTCA CACCCCGGTT AGGGCAGCTC 
TCTCTTACTT CATTGCCTTC TGCTGAATCT CGGGAACCTC TTTCAGCAGA GAGTGAGAGT 
AGAACTCCTT CAAGCCGTTT TCACATCCCG GTGTTGCAGG ATAGACTTTG GAAGTTATCA 
CAGCTTATAC AGCCTCAGCA AGATAACCTG AAGTCACTCC AAGAACAGTT AGCTACACAG 
AGGGAAGCCA TCATTCAGTC TAGGCAGGAA GCTCAGCAAG AATTTCTTTT GCACAAACAG 
GGTGAGTGGA AGGGAAGAGT GTCTCCAGAG CAGGTTGGCA CCTCCTCCTT CCTACCCCAA 
GTCATACAGC CTCTTGCTCC ATTACCTCCT AGTAAAGCTG GTAGAATCCA AGAACCTTGT 
TCAACTAAGA GTGATAATAT AGTCTCCTCA GGTCATTCTG AGATACCAAG GTTGCCTGAT 
AGACTTTTAG GTTTACCACA GCCTGTTTTG CCTCAACAAG ATTATCCAGT CACATTTCAA 
CAAGAACATT TGTATGCCCA GACAAACCCC CTTCCATGTA GCGAGAAAAT CCAAAGAGAG 
TTGGTTTTGC CCAGACAATA TACACTTGAG GGAAAGTCAC CTAAGCATTT TGTCCAACCT 
CACCATGGTG ATTTAAAGGC CTATCAACGG CAGTTAGATA TACAGAGGGA AGTCCAAGAA 
GAATTCCTTT TGCAAACACT AAGTAAATTG GAAAAAGGGG TCTCAGCTGA GCAGACCACC 
ACCTCTTCAT CCTTATCCCA CGTAGCACTG CCTGTTGCTG ACTCTAAAGG AATGCCAAAG 
TGTTTTCCAG CCAGAAGTGA CCTTGCTGTT TCCTCAAGTC ATCCTGAGAT TCTTAGACCT 
CAGGAGAGGC CTTTGCATTT ATCCCAGGCT GTTTTGCCTC AGCAAGACCA TGTGTCCACA 
CAGTTGGGCC TTCAGGGTGA AGTGCTACGT TTTAGTGAAA AGGCCCAGGA AGAACTGCTA 
AGAGGCAATC ACACAAAACT GATTGAAGGT GATGCTTCTG GGCAGCCTGT GCCCTCTTTG 
TTTCTACCCA AGGAAAGAGA ACATTCGTTT ATTCCACTAC CTTTTGCTGA GGTAAAATCT 
CAAAACATAC GTGAATTGTA TTCAGCCAAG ATTGAATGTG CAGCTCCCTC TAGTGCTTCT 
GTGAGCCCAA GACTTCAAGA TAGAATTTTG AGTTTTTCAC AACCCATCTT TGCTCAGCAA 
GATAACTGGG GACTTCAGAA GCAGTTGGAG CTACAAAAAG AAGTTCTGCC TTTTAGTCAG 
AAAGCCCAAG AAGTATTGCT TGTACCAACA CAGACAGCCT TGCAGCAACA GACACACAAA 
CATCGGGAGA CTCTGAAGGA TTTCTTCAAA GACAGCCAGG AAAGTAAGCC CACAGTTGAA 
AATGATTTTG AAACCCAGAA GCTCCAAGAA TGGCTTCCTC ATCTCCACGA TCAAAAAAAC 
ATTGGTCCTG CAGACAGGAG CAACTGTGAT GATTATCAGC TCCTTTCAGA AGGTGTGCAT 
CAGGACAAAG AACTGGGTAG GAAATCCTCA AAACCACCTG TAGTAAAAAT AAGAGGTGGA 
TTGGACTTGA ACCAACATGA ACTTAGTGCC ATACAAGAGG TAGAATCACC AACAAGTGGC 
AGAACTTCTA TACTAGGTAA ACCTGATTTT TATCAAGACA GAGACCCCCT AAGGGTCTCA 
ATAAGCCGAG AACAAAGTTT TCTTGGGAGC CCTCTGGACC GTGATCCATT TGGTCATCTT 
CACCCAGTTG CTCAGGAGAA CATCTGTGGT GATGACTCTG ATAAAGCAGT CAAAGTCAAG 
GAGGCTGTGG TTGAGAATTA TGCAGTATTA AGCTATGCTG TGGAGAGAGA AGGCCATACG 
TACCTGGATC CAAATGTGAA GTTAGATGTT AAGGCTGAAA CACAAGAAAT TTATCATGAG 
CCACTATCTT CAATAACTGT TTCCACTGGG AGTTTTTTAA GTTACGAAAA CATAGATTTG 
AGCCTTACAG AGCCAGAGTC ATTCCCAGAG CATGTGGACC ATAGGGAACA AGAACCTCCT 
GCCGGTAAAG AAGAGGAAAC AAATGTTTTA AGTTCTAGAG TTCCTTCAAC ACAAGATATT 
TATCAAAGAC AGGACTCTCA GGACGTCCAT AAACCTCTTT TATGTGCAGT GGAAACATTG 
ACATCTGGTC AGACACACAT TCAACAGATG AGAGACAATT ACATTAATGA AACAAATTTG 
ATGACTGAAA AAACAGACTT TCGGGTTGAC TTTGACTTTC CAGAATTGGA GCACAATTTT 
CCAAATTTGC ATCGTCAGCT ATTTAAACCC TTAGAACCAC ATCCAGATTT TGATTTGTTA 
TCATGTTTCT CTGGGATTTC TCAAGACAGT AAAGACTTTA ACCAGAGGTC AGGTTCTTCC 
TGTGAAAGCC TCCGTGCTAC TCCATCCCCC AAAAGTATGG CTTCTTTTAC AGCACTGAGG 
AGGGCCAGCC TGCATTCTTC TAACACCAGT CTGAACCAGC AGCCTGACCC TAACTTGGCT 
TGTGACACAG CTCAGAGTTT TGCTGTAGAA GATATTGAGG GGTCTGAACA ATCTTTTCGA 
CAACTTCGAC CAGAATATTC TTCACAGGAA GGGAGCCAGC ATGCCGATCT ACCAAGCATT 
TTTAGCATTG AAGCGAGAGC TTCTTCCCAA GATGTGGAAA ATCAGAACTG TTCCTCTGAA 
CATGAACTAT TACAAAACAA GCAAAAAAGT GTTCATTTCC AACTCTCAGT AGGAAATTTG 
CAAAGTTCAG TCTTCAATTC ATCTGGTGAG GCTAATGTAT TTCATCAGTT AAATCTACAG 
CATGGCACTA CATGTGGTTC TACCTCTAGT GAGTGGTCAA TAAAAGACCA ACCAGAAGGC 
AGAAAAGAAA GACTGGGTTT TGAAAAACTA TCAGAAAGAC TTGATACAGT GTTACAAGGT 
CAAGGACTCA CTGATGATAA AAAAGAAACC TGTGATTTAA ATCCACACAT AGAGGAAACT 
GATTCTCATT TATGTGTAAG GACAGTAGAG ATGGGAACTT CAATTCAGAT ACCATATTCA 
CTCACTGTTC AGAATGAAAA ATGTCTTCAG GACTCAACTA TGGCAGGAAT TCCAACAATC 
GTAGGAAACC CATCTCAACT TGCACAATCA GAGCTCTTTG TAAATTCTGG ATCATTTTCA 
TTACAGAGAT CTATTCCAGT CTGGGAAACA GAATCTGGCC ATGGTATAAT GGAAGAACCA 
GAACTTACAT TGACAAGCAC CAGTGATATC AGTATTGCTG AAATGGATTT TGCAAACTTA 
ACCCTAGAAG AAAAGAGAGA AAATGAGGCA AAGAGCTGTT TTCAGGTGAG TGAATTTCTG 
CCTCTTCTGT CAGAAACAGA AAACTCAGAT TATCCAGCCA TGTCAGAACA TCCACTGGAG 
AGACCAACTG TGTGTGGAGA AACCTTTTCA AGGTGTACAG CTGCACCAGA GAGCTTACAA 
GAAGCATTCA TAAAAAGAAA AAAGGCATTT ATAGAGAGAT CCTCACAGAG ACAGAAAGAG 
ATAAAGGACA AAGTTCATGC CTCTGGATAT TCTCAAACCA AAACAATAAC AGAGAAAACT 
ACAGGTTCCT TTGTGAGTGA CCTAAAGGGT ATGAGTAAAG TTAGGGTGTC GCTTCCTGAA 
GATAAAAAGA CTGCACAACC CCATACGCAC GAAAGGGCAG TAGGATTATA CCTTCAATTA 
GCTGAAATGA GAAAGCAGAA GGAAGACAAA GCAAAGCAAG ATGCACATGC CCAAAACAGA 
GCAAGGACAA AAGAATTTCA TAAGGTAAGC AGCATCCCAT ACATACCCCC AACTTTTCAT 
GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_015469903.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325389.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.

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CloneID	OBa52316
Clone ID Related Accession (Same CDS sequence)	XM_015469903.1 , XM_010820791.3 , XM_010820791.2
Accession Version	XM_015469903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646763.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015325389.1
CDS	33..6605
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQGLNNLFDNFDQNILHRKGASMPIYQAFLALKRELLPKMWKIRTVPLNMNYYKTSKKVFISNSQ

Target ORF information:

RefSeq Version	XM_015469903.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015469903.1

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820791.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819093.1 Bos taurus centrosomal protein 295 (CEP295), transcript variant X11, mRNA.

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CloneID	OBa52316
Clone ID Related Accession (Same CDS sequence)	XM_015469903.1 , XM_010820791.3 , XM_010820791.2
Accession Version	XM_010820791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819093.1
CDS	61..6633
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQGLNNLFDNFDQNILHRKGASMPIYQAFLALKRELLPKMWKIRTVPLNMNYYKTSKKVFISNSQ

Target ORF information:

RefSeq Version	XM_010820791.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295 (CEP295), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820791.3

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP295 ( XM_010820791.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819093.1 Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.

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CloneID	OBa52316
Clone ID Related Accession (Same CDS sequence)	XM_015469903.1 , XM_010820791.3 , XM_010820791.2
Accession Version	XM_010820791.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosomal protein of 295 kDa isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104628.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819093.1
CDS	33..6605
Translation	MTEVTAARRWPPRSRPNGCSEAAVGRAAGPSVSEPRFLRPGFRLPEPSYLKYYMKMKRKGVNAGKLRLSPNEEAFILKEDYERRRKLRLLQVREQERDIALQIREDIKQRRNQQFTRLAEELRTEWVESQTQKIKNLEKLYLASLRNMGEGHQQAKENEPDLNALAHRAAERKKKAEMRHKEALKVQKNQKEMLMKQKTWHIKARKEALLVEKERSAKIASLPPPPPALFENVEVKKTNVKTNSSTYHHLCTFVNREVDTKQPDPHLAAEEEAKRLEELQKQAAQEKMEQSEKAHARGFQAMKKIHLAQNQEKLMKELQQLQQEDLARRRLMVARMPPQLVELPYKRSEMKEDWQRELEFAFEDMYNADRKVKGNLILHLEPEPLPAVIDQIQDEELDLSMEHESLGETENIPMTEAETVHSSEADVPSAVKTHQIPSKILFKKLLNKIRSQKSLWTIKSVSEEEGEMITTVSEAESKALTVESGATVSEDRTLSSGQEQVVESDMLTIDSGPLTSEDKPLSFSTDSEKEQEMKTTQPLIAPSSILLHPQEEAVRVRMAARQKQIMEIEEQKQKQLELLEQIEQQKLRLETDCFRAQLEEEKRKKTQQTGVSTAPTSYTICSDEDRHRQMIHIYQQQLLQQNRFHKQSVETARKRLLEYQTMLRGKYPSMSATPVIPDSVTSVPPPKSERPSVPSEHEDQGQRPKLSPDKQPVQISKLEQDSQVSRQSRFPQRQVETTEALVTSDVLAKQALESEEHQKQLSQTEVQQRDYKLVAKDSQMLSRALSHDKPLTVQDARERAETSGATAFQSLDSQQVFSENNESVSSKLIEPSSFLPLVAEHSFSSLPTKVESGEIQQPSSTASKGVVSVSHSVAGQMQDKSLPFSENIIAQQSNLKALQEQLDLQKEVLRTRQEAQEQLLLCKQKELEGQTGLSIFLPLVPLNSFASLPSAQAESRRIQESSPTNKETGVSSGHPGVPQLQDRLNLKFFQEQLKTQRDSLQARREAQEVLCVHKQNELDGRVWAEQTGSSSLPPQVAQHTFTSLPSAGPQSGKIQDQYSSKTEKEHFSIQTEMPTSQDGSLGFPQQFQPLHDCLKLLQEQLITQRDALQARHEAQVELLSRKQRDLEDAKSVQMSSSFLPVVTQHSLASQVSAKTEPRRIQNFYFSERDSVIPSNNLVMPASQDASLSFPQYSLPRQENLTALHNQSHVQRVILGDKQETQEFVHKQSELEKIFPSEQTGTSLSQGAESERCQEYMSFRSDSTVPLSHWKIPRSQERLLGFSRHTQPLQGNLEGQQEQLDREEEALQFSQRSQGNVSSGQICPSFTPRLGQLSLTSLPSAESREPLSAESESRTPSSRFHIPVLQDRLWKLSQLIQPQQDNLKSLQEQLATQREAIIQSRQEAQQEFLLHKQGEWKGRVSPEQVGTSSFLPQVIQPLAPLPPSKAGRIQEPCSTKSDNIVSSGHSEIPRLPDRLLGLPQPVLPQQDYPVTFQQEHLYAQTNPLPCSEKIQRELVLPRQYTLEGKSPKHFVQPHHGDLKAYQRQLDIQREVQEEFLLQTLSKLEKGVSAEQTTTSSSLSHVALPVADSKGMPKCFPARSDLAVSSSHPEILRPQERPLHLSQAVLPQQDHVSTQLGLQGEVLRFSEKAQEELLRGNHTKLIEGDASGQPVPSLFLPKEREHSFIPLPFAEVKSQNIRELYSAKIECAAPSSASVSPRLQDRILSFSQPIFAQQDNWGLQKQLELQKEVLPFSQKAQEVLLVPTQTALQQQTHKHRETLKDFFKDSQESKPTVENDFETQKLQEWLPHLHDQKNIGPADRSNCDDYQLLSEGVHQDKELGRKSSKPPVVKIRGGLDLNQHELSAIQEVESPTSGRTSILGKPDFYQDRDPLRVSISREQSFLGSPLDRDPFGHLHPVAQENICGDDSDKAVKVKEAVVENYAVLSYAVEREGHTYLDPNVKLDVKAETQEIYHEPLSSITVSTGSFLSYENIDLSLTEPESFPEHVDHREQEPPAGKEEETNVLSSRVPSTQDIYQRQDSQDVHKPLLCAVETLTSGQTHIQQMRDNYINETNLMTEKTDFRVDFDFPELEHNFPNLHRQLFKPLEPHPDFDLLSCFSGISQDSKDFNQGLNNLFDNFDQNILHRKGASMPIYQAFLALKRELLPKMWKIRTVPLNMNYYKTSKKVFISNSQ

Target ORF information:

RefSeq Version	XM_010820791.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 295kDa (CEP295), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820791.2

ORF Insert Sequence:

ATGACGGAAG TGACGGCGGC TCGGAGATGG CCGCCTCGCT CACGTCCGAA CGGCTGTTCG 
GAGGCAGCTG TGGGGCGGGC CGCCGGGCCT TCTGTGTCTG AGCCGCGATT CCTGAGGCCG 
GGCTTTCGTC TCCCTGAACC TTCATATTTA AAGTACTACA TGAAAATGAA AAGAAAGGGA 
GTGAATGCTG GCAAGCTGAG ATTGAGTCCT AATGAGGAAG CCTTCATTTT AAAGGAAGAT 
TATGAAAGAA GACGAAAACT AAGATTGCTG CAGGTTCGAG AACAAGAAAG AGATATTGCT 
TTACAGATAA GAGAAGATAT AAAACAGAGG AGAAATCAGC AATTCACTCG TTTGGCAGAG 
GAACTAAGGA CAGAATGGGT GGAATCACAA ACTCAGAAAA TCAAGAACTT GGAAAAACTG 
TATTTGGCAA GTTTAAGAAA TATGGGAGAG GGGCATCAAC AAGCCAAAGA AAATGAGCCT 
GATTTAAATG CTCTGGCACA CCGGGCAGCA GAAAGGAAAA AAAAGGCAGA AATGAGACAT 
AAAGAGGCCC TGAAAGTACA GAAAAATCAA AAAGAAATGT TAATGAAACA AAAAACCTGG 
CATATAAAAG CTCGAAAAGA AGCTCTGCTT GTGGAAAAGG AAAGATCTGC CAAAATTGCA 
AGTCTGCCAC CCCCTCCTCC AGCTCTCTTT GAGAATGTTG AAGTAAAGAA AACTAACGTG 
AAGACGAACA GTTCTACCTA CCATCATCTT TGTACTTTTG TGAATAGAGA GGTGGACACA 
AAGCAGCCAG ATCCTCATTT GGCTGCTGAA GAAGAAGCTA AAAGATTAGA AGAACTACAG 
AAGCAGGCAG CGCAAGAGAA AATGGAACAG AGTGAGAAGG CACATGCCCG GGGATTCCAA 
GCAATGAAAA AGATCCATTT GGCTCAAAAT CAAGAGAAAC TAATGAAAGA ACTCCAGCAG 
CTCCAGCAAG AGGACCTGGC ACGCAGGAGA CTGATGGTAG CACGGATGCC GCCACAACTA 
GTGGAACTTC CATACAAACG CAGTGAAATG AAAGAAGATT GGCAGAGAGA GCTAGAATTT 
GCCTTTGAAG ACATGTACAA TGCAGACAGA AAGGTGAAAG GAAACCTGAT TTTGCACCTT 
GAACCAGAGC CTCTGCCTGC TGTGATTGAT CAGATCCAAG ACGAAGAACT GGACCTCTCA 
ATGGAACATG AAAGTTTAGG GGAGACTGAA AACATTCCAA TGACAGAAGC TGAAACAGTA 
CATTCTAGTG AAGCAGACGT TCCCTCAGCA GTGAAGACCC ACCAGATTCC TTCAAAAATT 
CTTTTTAAAA AATTATTAAA TAAGATCCGA AGCCAAAAAT CTCTCTGGAC AATTAAATCC 
GTGTCTGAAG AGGAGGGTGA AATGATTACA ACTGTCAGTG AAGCTGAAAG TAAAGCCCTG 
ACAGTTGAAT CAGGAGCAAC TGTGAGTGAA GACAGAACAT TATCCTCTGG GCAGGAACAA 
GTTGTTGAAA GTGACATGCT AACAATTGAC TCTGGACCAC TTACTAGTGA AGATAAACCA 
CTTTCATTTA GTACTGACTC TGAAAAGGAA CAAGAAATGA AAACCACTCA GCCTCTCATA 
GCTCCAAGTT CAATTCTACT TCATCCTCAG GAAGAAGCAG TCAGAGTTAG AATGGCAGCG 
AGGCAGAAAC AGATAATGGA AATAGAAGAA CAGAAGCAAA AGCAGTTGGA ATTGCTTGAA 
CAGATTGAAC AACAGAAGTT AAGATTAGAA ACTGATTGCT TCAGAGCTCA GCTGGAAGAA 
GAAAAAAGAA AAAAAACTCA ACAGACTGGG GTCAGCACTG CTCCAACATC ATACACCATA 
TGTTCTGATG AAGATAGGCA CAGGCAGATG ATTCATATTT ATCAGCAACA GCTATTACAG 
CAAAACAGGT TTCACAAGCA GTCTGTTGAA ACAGCCAGGA AGCGATTACT TGAATATCAG 
ACAATGTTAA GAGGAAAGTA CCCATCCATG TCAGCTACGC CAGTGATACC TGATTCTGTT 
ACATCAGTGC CACCACCAAA ATCTGAAAGA CCCAGCGTTC CATCAGAACA TGAGGATCAA 
GGTCAGAGAC CCAAACTGAG TCCTGACAAA CAACCTGTAC AGATCTCCAA ATTAGAGCAA 
GATTCTCAGG TTTCAAGACA GAGTCGCTTT CCACAAAGAC AGGTAGAAAC AACTGAAGCA 
TTAGTCACTT CAGATGTTTT AGCCAAGCAA GCTTTGGAAT CAGAAGAACA CCAAAAGCAA 
CTCTCACAGA CTGAAGTACA ACAGAGAGAC TACAAATTGG TCGCTAAAGA TTCTCAAATG 
CTTTCAAGGG CTTTATCACA TGATAAGCCA CTGACAGTAC AGGATGCTAG AGAAAGAGCT 
GAAACATCTG GGGCAACAGC TTTTCAAAGT TTAGACTCCC AGCAAGTGTT CTCAGAGAAC 
AATGAAAGTG TATCTTCTAA GTTAATTGAA CCATCTTCAT TCTTACCATT GGTAGCTGAG 
CATTCTTTTA GTTCTCTGCC TACTAAAGTC GAGTCTGGAG AAATCCAGCA GCCCTCTTCA 
ACTGCGAGCA AAGGTGTAGT TTCTGTAAGC CACTCTGTAG CTGGCCAAAT GCAGGATAAG 
TCTTTGCCAT TCTCGGAAAA TATCATAGCC CAGCAAAGTA ATTTGAAGGC TCTCCAAGAA 
CAGTTAGACC TACAGAAGGA AGTTCTTCGG ACAAGACAGG AAGCTCAGGA ACAATTGCTT 
TTGTGTAAAC AGAAAGAGTT GGAAGGGCAA ACTGGCCTTT CTATATTCCT TCCTTTAGTA 
CCTCTGAATT CATTTGCTTC ACTGCCTTCA GCCCAAGCTG AGTCAAGGAG AATCCAGGAA 
TCTTCTCCAA CCAATAAAGA GACTGGTGTT TCCTCAGGCC ATCCTGGGGT CCCACAACTT 
CAGGATAGGC TTAATTTAAA ATTTTTCCAA GAACAGTTAA AAACGCAGAG GGACAGCCTT 
CAGGCTAGGC GGGAAGCCCA GGAAGTATTA TGTGTGCATA AACAGAATGA GTTGGATGGG 
AGAGTATGGG CTGAACAGAC TGGGTCCTCT TCTCTCCCAC CTCAAGTAGC TCAGCATACA 
TTTACTTCAC TGCCTTCTGC TGGTCCTCAG TCTGGAAAAA TCCAGGACCA GTATTCATCT 
AAGACTGAGA AGGAGCATTT CTCAATCCAG ACTGAAATGC CCACATCTCA GGATGGGTCT 
TTAGGTTTCC CACAACAGTT CCAACCTTTG CACGATTGTT TGAAGTTGCT CCAAGAGCAG 
CTGATTACAC AGAGGGATGC TCTCCAGGCC AGGCATGAAG CCCAAGTGGA ATTACTTTCA 
CGTAAACAAA GGGATTTGGA AGACGCTAAG TCTGTGCAGA TGAGTTCTTC GTTCCTGCCA 
GTGGTCACTC AACATTCACT TGCTTCACAA GTTTCAGCTA AAACTGAGCC TAGGAGAATT 
CAGAACTTTT ATTTCTCTGA GAGGGATAGT GTTATTCCCT CAAATAATTT GGTAATGCCA 
GCATCTCAGG ATGCGTCTCT TAGTTTTCCA CAGTATAGTC TGCCACGGCA GGAAAATTTA 
ACAGCACTCC ACAATCAGTC ACACGTTCAG AGGGTAATAC TTGGCGATAA ACAAGAAACT 
CAGGAATTTG TACACAAACA AAGTGAATTA GAAAAAATTT TCCCTTCTGA ACAGACTGGC 
ACTTCTTTGT CTCAGGGAGC TGAATCTGAA AGATGCCAGG AATACATGTC ATTCAGGAGT 
GACAGTACTG TTCCCTTGAG CCACTGGAAG ATCCCAAGAT CTCAGGAAAG ACTTCTGGGA 
TTTTCACGAC ATACACAACC TCTGCAAGGT AATTTGGAAG GACAGCAAGA ACAGTTAGAC 
AGAGAAGAGG AGGCCCTTCA GTTCAGCCAG AGATCCCAAG GGAATGTATC TTCTGGACAG 
ATTTGCCCCT CGTTCACACC CCGGTTAGGG CAGCTCTCTC TTACTTCATT GCCTTCTGCT 
GAATCTCGGG AACCTCTTTC AGCAGAGAGT GAGAGTAGAA CTCCTTCAAG CCGTTTTCAC 
ATCCCGGTGT TGCAGGATAG ACTTTGGAAG TTATCACAGC TTATACAGCC TCAGCAAGAT 
AACCTGAAGT CACTCCAAGA ACAGTTAGCT ACACAGAGGG AAGCCATCAT TCAGTCTAGG 
CAGGAAGCTC AGCAAGAATT TCTTTTGCAC AAACAGGGTG AGTGGAAGGG AAGAGTGTCT 
CCAGAGCAGG TTGGCACCTC CTCCTTCCTA CCCCAAGTCA TACAGCCTCT TGCTCCATTA 
CCTCCTAGTA AAGCTGGTAG AATCCAAGAA CCTTGTTCAA CTAAGAGTGA TAATATAGTC 
TCCTCAGGTC ATTCTGAGAT ACCAAGGTTG CCTGATAGAC TTTTAGGTTT ACCACAGCCT 
GTTTTGCCTC AACAAGATTA TCCAGTCACA TTTCAACAAG AACATTTGTA TGCCCAGACA 
AACCCCCTTC CATGTAGCGA GAAAATCCAA AGAGAGTTGG TTTTGCCCAG ACAATATACA 
CTTGAGGGAA AGTCACCTAA GCATTTTGTC CAACCTCACC ATGGTGATTT AAAGGCCTAT 
CAACGGCAGT TAGATATACA GAGGGAAGTC CAAGAAGAAT TCCTTTTGCA AACACTAAGT 
AAATTGGAAA AAGGGGTCTC AGCTGAGCAG ACCACCACCT CTTCATCCTT ATCCCACGTA 
GCACTGCCTG TTGCTGACTC TAAAGGAATG CCAAAGTGTT TTCCAGCCAG AAGTGACCTT 
GCTGTTTCCT CAAGTCATCC TGAGATTCTT AGACCTCAGG AGAGGCCTTT GCATTTATCC 
CAGGCTGTTT TGCCTCAGCA AGACCATGTG TCCACACAGT TGGGCCTTCA GGGTGAAGTG 
CTACGTTTTA GTGAAAAGGC CCAGGAAGAA CTGCTAAGAG GCAATCACAC AAAACTGATT 
GAAGGTGATG CTTCTGGGCA GCCTGTGCCC TCTTTGTTTC TACCCAAGGA AAGAGAACAT 
TCGTTTATTC CACTACCTTT TGCTGAGGTA AAATCTCAAA ACATACGTGA ATTGTATTCA 
GCCAAGATTG AATGTGCAGC TCCCTCTAGT GCTTCTGTGA GCCCAAGACT TCAAGATAGA 
ATTTTGAGTT TTTCACAACC CATCTTTGCT CAGCAAGATA ACTGGGGACT TCAGAAGCAG 
TTGGAGCTAC AAAAAGAAGT TCTGCCTTTT AGTCAGAAAG CCCAAGAAGT ATTGCTTGTA 
CCAACACAGA CAGCCTTGCA GCAACAGACA CACAAACATC GGGAGACTCT GAAGGATTTC 
TTCAAAGACA GCCAGGAAAG TAAGCCCACA GTTGAAAATG ATTTTGAAAC CCAGAAGCTC 
CAAGAATGGC TTCCTCATCT CCACGATCAA AAAAACATTG GTCCTGCAGA CAGGAGCAAC 
TGTGATGATT ATCAGCTCCT TTCAGAAGGT GTGCATCAGG ACAAAGAACT GGGTAGGAAA 
TCCTCAAAAC CACCTGTAGT AAAAATAAGA GGTGGATTGG ACTTGAACCA ACATGAACTT 
AGTGCCATAC AAGAGGTAGA ATCACCAACA AGTGGCAGAA CTTCTATACT AGGTAAACCT 
GATTTTTATC AAGACAGAGA CCCCCTAAGG GTCTCAATAA GCCGAGAACA AAGTTTTCTT 
GGGAGCCCTC TGGACCGTGA TCCATTTGGT CATCTTCACC CAGTTGCTCA GGAGAACATC 
TGTGGTGATG ACTCTGATAA AGCAGTCAAA GTCAAGGAGG CTGTGGTTGA GAATTATGCA 
GTATTAAGCT ATGCTGTGGA GAGAGAAGGC CATACGTACC TGGATCCAAA TGTGAAGTTA 
GATGTTAAGG CTGAAACACA AGAAATTTAT CATGAGCCAC TATCTTCAAT AACTGTTTCC 
ACTGGGAGTT TTTTAAGTTA CGAAAACATA GATTTGAGCC TTACAGAGCC AGAGTCATTC 
CCAGAGCATG TGGACCATAG GGAACAAGAA CCTCCTGCCG GTAAAGAAGA GGAAACAAAT 
GTTTTAAGTT CTAGAGTTCC TTCAACACAA GATATTTATC AAAGACAGGA CTCTCAGGAC 
GTCCATAAAC CTCTTTTATG TGCAGTGGAA ACATTGACAT CTGGTCAGAC ACACATTCAA 
CAGATGAGAG ACAATTACAT TAATGAAACA AATTTGATGA CTGAAAAAAC AGACTTTCGG 
GTTGACTTTG ACTTTCCAGA ATTGGAGCAC AATTTTCCAA ATTTGCATCG TCAGCTATTT 
AAACCCTTAG AACCACATCC AGATTTTGAT TTGTTATCAT GTTTCTCTGG GATTTCTCAA 
GACAGTAAAG ACTTTAACCA GGGTCTGAAC AATCTTTTCG ACAACTTCGA CCAGAATATT 
CTTCACAGGA AGGGAGCCAG CATGCCGATC TACCAAGCAT TTTTAGCATT GAAGCGAGAG 
CTTCTTCCCA AGATGTGGAA AATCAGAACT GTTCCTCTGA ACATGAACTA TTACAAAACA 
AGCAAAAAAG TGTTCATTTC CAACTCTCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CEP295
Entrez Gene ID	532848
Full Name	centrosomal protein 295
General protein information	Preferred Names centrosomal protein 295 Names centrosomal protein of 295 kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	KIAA1731	NP_203753.1
Pan troglodytes (chimpanzee)	KIAA1731	XP_508695.3
Bos taurus (cattle)	KIAA1731	XP_003588053.2
Mus musculus (house mouse)	5830418K08Rik	NP_795950.2
	LOC697392	XP_001085979.2
Rattus norvegicus (Norway rat)	RGD1311723	NP_001257977.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CEP295 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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