MICAL3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL3
Entrez Gene ID	532244
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 3
Synonyms	MICAL-3
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 3 Names [F-actin]-monooxygenase MICAL3 protein-methionine sulfoxide oxidase MICAL3 Molecule interacting with CasL protein 3 Protein-methionine sulfoxide oxidase MICAL3 [F-actin]-methionine sulfoxide oxidase MICAL3 microtubule associated monoxygenase, calponin and LIM domain containing 3
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463589.1	XP_015319075.1	protein-methionine sulfoxide oxidase MICAL3 isoform X6
XM_010805726.2	XP_010804028.1	protein-methionine sulfoxide oxidase MICAL3 isoform X6
XM_005207355.3	XP_005207412.1	protein-methionine sulfoxide oxidase MICAL3 isoform X10
XM_010805720.3	XP_010804022.2	F-actin-monooxygenase MICAL3 isoform X1
XM_010805721.3	XP_010804023.2	F-actin-monooxygenase MICAL3 isoform X1
XM_024992784.1	XP_024848552.1	F-actin-monooxygenase MICAL3 isoform X14
XM_010805721.2	XP_010804023.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_015471384.1	XP_015326870.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_010805724.2	XP_010804026.1	protein-methionine sulfoxide oxidase MICAL3 isoform X4
XM_015463583.1	XP_015319069.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_015463584.1	XP_015319070.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_015463587.1	XP_015319073.1	protein-methionine sulfoxide oxidase MICAL3 isoform X3
XM_010827969.2	XP_010826271.2	protein-methionine sulfoxide oxidase MICAL3 isoform X5
XM_024992773.1	XP_024848541.1	F-actin-monooxygenase MICAL3 isoform X3
XM_024992781.1	XP_024848549.1	F-actin-monooxygenase MICAL3 isoform X11
XM_010805720.2	XP_010804022.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_015463592.1	XP_015319078.1	protein-methionine sulfoxide oxidase MICAL3 isoform X9
XM_015463595.1	XP_015319081.1	protein-methionine sulfoxide oxidase MICAL3 isoform X12
XM_010805725.2	XP_010804027.1	protein-methionine sulfoxide oxidase MICAL3 isoform X5
XM_010805730.2	XP_010804032.1	protein-methionine sulfoxide oxidase MICAL3 isoform X9
XM_002687919.5	XP_002687965.2	protein-methionine sulfoxide oxidase MICAL3 isoform X12
XM_015463591.1	XP_015319077.1	protein-methionine sulfoxide oxidase MICAL3 isoform X8
XM_024992774.1	XP_024848542.1	F-actin-monooxygenase MICAL3 isoform X4
XM_024992776.1	XP_024848544.1	F-actin-monooxygenase MICAL3 isoform X6
XM_024992777.1	XP_024848545.1	F-actin-monooxygenase MICAL3 isoform X7
XM_024992782.1	XP_024848550.1	F-actin-monooxygenase MICAL3 isoform X12
XM_010805723.2	XP_010804025.1	protein-methionine sulfoxide oxidase MICAL3 isoform X3
XM_010805722.2	XP_010804024.1	protein-methionine sulfoxide oxidase MICAL3 isoform X2
XM_015463586.1	XP_015319072.1	protein-methionine sulfoxide oxidase MICAL3 isoform X2
XM_015463588.1	XP_015319074.1	protein-methionine sulfoxide oxidase MICAL3 isoform X4
XM_015463593.1	XP_015319079.1	protein-methionine sulfoxide oxidase MICAL3 isoform X10
XM_024992775.1	XP_024848543.1	F-actin-monooxygenase MICAL3 isoform X5
XM_024992778.1	XP_024848546.1	F-actin-monooxygenase MICAL3 isoform X8
XM_010805727.2	XP_010804029.1	protein-methionine sulfoxide oxidase MICAL3 isoform X7
XM_010805729.2	XP_010804031.1	protein-methionine sulfoxide oxidase MICAL3 isoform X8
XM_005207361.3	XP_005207418.1	protein-methionine sulfoxide oxidase MICAL3 isoform X11
XM_015463594.1	XP_015319080.1	protein-methionine sulfoxide oxidase MICAL3 isoform X11
XM_015463585.1	XP_015319071.1	protein-methionine sulfoxide oxidase MICAL3 isoform X1
XM_015463590.1	XP_015319076.1	protein-methionine sulfoxide oxidase MICAL3 isoform X7
XM_024992772.1	XP_024848540.1	F-actin-monooxygenase MICAL3 isoform X2
XM_024992779.1	XP_024848547.1	F-actin-monooxygenase MICAL3 isoform X9
XM_024992780.1	XP_024848548.1	F-actin-monooxygenase MICAL3 isoform X10
XM_024992783.1	XP_024848551.1	F-actin-monooxygenase MICAL3 isoform X13

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mical3	NP_001178014.1
Pan troglodytes (chimpanzee)	MICAL3	XP_001151830.2
	MICAL3	XP_001103660.2
Canis lupus familiaris (dog)	MICAL3	XP_005637453.1
Bos taurus (cattle)	MICAL3	XP_005207412.1
Danio rerio (zebrafish)	mical3a	XP_005159086.1
Homo sapiens (human)	MICAL3	NP_056056.2
Mus musculus (house mouse)	Mical3	NP_001257404.1
Gallus gallus (chicken)	MICAL3	XP_004938010.1
Xenopus tropicalis (tropical clawed frog)	mical3	XP_004912548.1

Gene Ontology

The following MICAL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234968	XM_024992784.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa234955	XM_010805721.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234955	XM_010805720.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234956	XM_024992772.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234957	XM_024992773.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234958	XM_024992774.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234959	XM_024992775.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234960	XM_024992776.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234961	XM_024992777.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234962	XM_024992778.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234963	XM_024992779.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234964	XM_024992780.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234965	XM_024992781.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234966	XM_024992782.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234967	XM_024992783.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa19955	XM_015463593.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa19955	XM_005207355.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa19958	XM_015463594.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19958	XM_005207361.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19960	XM_002687919.5 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa19960	XM_015463595.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa52131	XM_015471384.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52131	XM_015463585.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52131	XM_015463584.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52131	XM_010805720.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52131	XM_015463583.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52131	XM_010805721.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52132	XM_010805722.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52132	XM_015463586.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52133	XM_010805723.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52133	XM_015463587.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52134	XM_010805724.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52134	XM_015463588.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52135	XM_010805725.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52135	XM_010827969.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52136	XM_015463589.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52136	XM_010805726.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52137	XM_010805727.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52137	XM_015463590.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52139	XM_015463591.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52139	XM_010805729.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52140	XM_015463592.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa52140	XM_010805730.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL3 ( XM_024992784.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848552.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.

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CloneID	OBa234968
Clone ID Related Accession (Same CDS sequence)	XM_024992784.1
Accession Version	XM_024992784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
GAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848552.1
CDS	1..5883
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992784.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992784.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
GAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805720.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804022.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

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CloneID	OBa234955
Clone ID Related Accession (Same CDS sequence)	XM_010805720.3 , XM_010805721.3
Accession Version	XM_010805720.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805720.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804022.2
CDS	272..6739
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805720.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805720.3

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805721.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804023.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

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CloneID	OBa234955
Clone ID Related Accession (Same CDS sequence)	XM_010805720.3 , XM_010805721.3
Accession Version	XM_010805721.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805721.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804023.2
CDS	517..6984
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805721.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805721.3

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992772.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848540.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

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CloneID	OBa234956
Clone ID Related Accession (Same CDS sequence)	XM_024992772.1
Accession Version	XM_024992772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848540.1
CDS	1..6465
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992772.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992772.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992773.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848541.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

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CloneID	OBa234957
Clone ID Related Accession (Same CDS sequence)	XM_024992773.1
Accession Version	XM_024992773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848541.1
CDS	1..6453
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYTDLIDLHLGGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992773.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992773.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992774.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848542.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

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CloneID	OBa234958
Clone ID Related Accession (Same CDS sequence)	XM_024992774.1
Accession Version	XM_024992774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848542.1
CDS	1..6405
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992774.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992774.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992775.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848543.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

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CloneID	OBa234959
Clone ID Related Accession (Same CDS sequence)	XM_024992775.1
Accession Version	XM_024992775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848543.1
CDS	1..6402
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992775.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992775.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCG AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992776.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848544.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

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CloneID	OBa234960
Clone ID Related Accession (Same CDS sequence)	XM_024992776.1
Accession Version	XM_024992776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACACTGACC TCATCGACTT GCATCTGGGT GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848544.1
CDS	1..6390
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYTDLIDLHLGGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992776.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992776.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCGAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG ACTATTGGGG AGAGTCTTAT 
TACACTGACC TCATCGACTT GCATCTGGGT GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992777.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848545.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

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CloneID	OBa234961
Clone ID Related Accession (Same CDS sequence)	XM_024992777.1
Accession Version	XM_024992777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CGAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848545.1
CDS	1..6360
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992777.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992777.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CGAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992778.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848546.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

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CloneID	OBa234962
Clone ID Related Accession (Same CDS sequence)	XM_024992778.1
Accession Version	XM_024992778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CGAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848546.1
CDS	1..6330
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992778.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992778.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CGAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992779.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848547.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

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CloneID	OBa234963
Clone ID Related Accession (Same CDS sequence)	XM_024992779.1
Accession Version	XM_024992779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGAAGTAT ATAAAGATGT ACACGGGCGG AGTGAGCTCA 
TTGGCTGAGC AGATAGCCAA TCAGCTTCAA AGGAAAGAAC AACCCAAAAC CCTTCTAGAC 
AAGAAGGAAC TGGGCTCCAT AAAGAAGGAG TTCCCGCAGA ACTTGGGAGG CAGCGACACC 
TGCTATTTCT GCCAGAAGCG GGTCTATGTG ATGGAGCGGC TGAGCGCCGA GGGCAAGTTC 
TTCCACCGGA GCTGCTTCAA GTGCGAGCAC TGCGCCACCA CCCTGCGCCT GTCGGCCTAT 
GCCTACGCCC TCGAGGACGG TAAATTCTAC TGCAAGCCAC ACTACTGTTA CCGGCTGTCT 
GGCCCTGCAC AGAGGAAGAG ACCGGCAGGG GTGCCTCTGT CTGGAAAGGA GGCCCGGGGA 
CCCCTGCAGG ACAGCCCCGC CGCAGACGCC AGTGGACGGC CCAGCACCAG TGCCAGCCCT 
GCCGAGAGAA GCCCAGGCCC AAGCGTGAAT GGCCTGGAGG AGCCGAGCGT CGCCAAGAGA 
CTGCGGGGCA CCCCGGAGAG GATCGAGCTG GAGAACTACC GGCTGTCGGT GCGGCAGGCG 
GAGGGGCTGG AGGAGGTGCC CGAGGAGACG CAGGCCGAGC ACAACCTGAG CAGCGTGCTG 
GACACGGGCA CAGAGGAGGA CGCGGCCAGC AGCAGTTCGG AGTCGGAGAT GGAGGAAGAG 
GAGCCTCCGC TGCCCACCTC GGACCTGGGC GGCGTACCCT GGAAGGAGGC TGTGAGGATC 
CATGCGCTCC TGAGAGGGAA GAGTGAAGAG GAGCTGGAGG CCTCCAGGAG CTTCGGGGCC 
GGGGAGGAGG AGGAGGAAGA CGAGGAGGAC GAGGAGGAGG AGGAGGAGGA GGAAGACGAG 
GAGGACGAGG AGGAGGACGA GGATGAGTCT AGTGAAGTCG GGAGCCCAAG GAGGCTGCAG 
CAGCTGCTGA ACCCGGCCGA CCCCCTGGAG ATCCAGGCCG ACGTGCACTG GACGCATATC 
CGTGAGAGCC AGGAGGAGCG AGCGGCACTG GCGCCCAAGT CCCCCCTTCC CGGAGTGCCC 
GGCCTCCCCT CCGTCCGCCG CGCAGCAGTT CAGGCCTGGC TGGAGTCGGT CTCCGGAGTC 
CCATTCGATG AGGATGACCT GGAGCGAGAC GTGGACTCGG AACCAGCAGA GATCGAAGGG 
GAGGCAGCGG AGAATGGGGA CGCAGGGGAC ACTGGTGCCG AGCTGGACGA CGATCAGCAC 
TGGTCGGATG ACGTCCCGTC CGAGGCCAAC ACGGAGCTGC ACCTGCCGGC AGTGGGAGCA 
GAGCTGGAGC TGAGGGTGTC GGATGGCGAG GAGGAGCCAC CGCCGGCCTC AGTAGGGCAC 
CCAGAGAGAG GTCCCTCCCG AGTCTCCAGC CCCACCCGGT CCCCTGAGGA GCCCACGGGC 
CTGTCCTCCC CAGCCCGCAG CCCCGGGGCA CAGAGCGCCC ATCCCCCACT TGCCGCTGTG 
GCCACCGGGG TAAGGTCACC CGCCGAGAGC CCTCTGCCGG AGCCCTCGAC CCCGCCAGCG 
GAGCCCGAAG CTCACCCTCC CATCCGATCG CAGCCTGAAG CCAGGACGCC ACCCTCGCCT 
GCCAGCCCCC AGCGCCCATC CCCGCCCACC CAACTGCCCA TCTGCTCCCA GCCTCAGCCG 
TCCCCTGAGG CCACCGTACC GTCCCCCACC CTGTCCCCCA TCCGCTCCCA GCCCGTGCCG 
GCCAGAACCT CCACTCCTCT GGCCCCCCTC CCCGTGAAGA ACCAAGGGGT CACGAAGGAC 
ACACTGGGCA GCTCCCTCCC TGGGGACGAG GCCCTGAAAC GCAGCGACCT GGTGGCAGAG 
TTCTGGATGA AGAGTGCCGA GATCCGCCGC AGCCTGGGGC TCACGCCTGT GCACCGGAGC 
CCGGGGTCTG AGCTCGCCTT CCAGTCCCCG CCCCTGAAGG CCTGTCCAGC AGAGAAGGCC 
CCCCAGAGCG AGGGGCTCCG GCTCCTAAAA CCCCCACCTG TCCCCAGGAA GCTGGGGCTG 
CCAGCAGCCG AGGGGGCCCA GCCCTGCCCA CCCACCCCTG TGTCCCCGCC CGACAGGGAG 
CCAAAGGGCC CCCGGGAGGA GCACAGAGAC CTGTCCAGCA GCTCGGGGCT GGGCCTGCAG 
GGCAGCTCCT CCCGCACCAG GACACCGGGC AGCCAGAGCT TCAACACCTC TGACTCCACC 
ATGCTCACGC CTCCTTCCAG CCCGCCGCCC CCGCCGCCTG ACGAGGAGCC CGCTACCCTT 
CACAGGAAGC CAGCCCTGGC AGGGCAGCTT GTGGCCTCAG CACCCCCACC CCCGGCTGTA 
TGTGTGAGGC CCCCGCGGGA GCCCACCCAG CCCCCCCAGG AGGAGGCGCG GAAGTCTTTC 
GTGGAGAGCG TGGATGAGAT TCCCTTTGCG GATGATGTGG AGGACACGTA TGATGACAAC 
ACATGTGACG ACCGCACGGA GGACTCGAGT CTGCAGGAGA CCTTCTTCAC ACCCCCCTCC 
CACTGGCCCC ACCCCAAGCA GCCCTTGGCT CCGGAGAACG GCCGGGGGCC AGAGAGTGCG 
GTCCCACTGC AGAAGCGGGG GCTGCCGCTG GTCTCGGCCG AGGCCAAGGA GCTGGCGGCC 
GAGCGCATGC GGGCCCGGGA GAAGTCGGTG CGGAGCCAGG CACTGAGGGA CGCCATGGCC 
CGGCAGCTGA GCAGGATGAA GGAGATGGAC ATAGCAGCTG CAGCCCCCAG GACCCCCAGG 
ACCCCTGCAC CCCGCAGAGC CACTGCCGTG CCCCCCAAGG GCCCCGAGGA ACCTGCCCCG 
AGGCACGAAG CCACAAGTGA GGAGCTGCTG TCCCCTCCCT CGGACTCAGG GGGCCCGGAC 
GGCTCGGTCA CCTCGTCAGA GGGCTCCAGT GGGAAGAGCA AGAAGAGGTC GTCCCTCTTC 
TCTCCTCGGA GAAGCAAGAA AGAGAAGAAG CCCAAAGGCG AGGGCCGGCC CCTGGAGAGG 
CCCAGCCCCG GCACTCTGGA GGAAGCGGCC GCCAAGCCCA GGTCTCTGTG GAAGTCTGTC 
TTCTCGGGGT ACAGGAAGGA CAAGAAGAAG AGTGATGGCC GGTCTTGCCC CAGCACCCCG 
TCCAGCGGGA CCACAGTGGA CGCCGGCAAG CCCAGGGCAT CTCCCGTCTC AAGAGCAGAG 
CTGCGGACGC GGCGCCAGCT GAGCTGCTCG GAGGACTCGG ACCTCTCCAG TGACGACGTC 
CTCGAGCGGA CCTCCCAGAA GTCCAGAAAA GAGCCGAGAA CTTACACGGA GGAGGAGCTG 
AACGCCAAGC TGACCCGGCG TGTGCAGAAG GCAGCTCGGA GGCAGGCCAA GCAGGAGGAG 
CTGAAACGGC TGCACCGCGC GCAGATCATC CAGCGGCAGC TGGAGCAGGT GGAGGAGAAG 
CAGCGGCAGC TGGAGGAGAG GGGGGTCGCC GTGGAGAAGG CGCTGCGCGG GGAAGCAGTT 
GAGCCCAGTG GCGGGACTCC ACGGCGTAGA CCTCTCTCCT TCTGCCCTTG CTGTGTCCAG 
GAAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848547.1
CDS	1..6303
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992779.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992779.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGAAGTAT ATAAAGATGT ACACGGGCGG AGTGAGCTCA 
TTGGCTGAGC AGATAGCCAA TCAGCTTCAA AGGAAAGAAC AACCCAAAAC CCTTCTAGAC 
AAGAAGGAAC TGGGCTCCAT AAAGAAGGAG TTCCCGCAGA ACTTGGGAGG CAGCGACACC 
TGCTATTTCT GCCAGAAGCG GGTCTATGTG ATGGAGCGGC TGAGCGCCGA GGGCAAGTTC 
TTCCACCGGA GCTGCTTCAA GTGCGAGCAC TGCGCCACCA CCCTGCGCCT GTCGGCCTAT 
GCCTACGCCC TCGAGGACGG TAAATTCTAC TGCAAGCCAC ACTACTGTTA CCGGCTGTCT 
GGCCCTGCAC AGAGGAAGAG ACCGGCAGGG GTGCCTCTGT CTGGAAAGGA GGCCCGGGGA 
CCCCTGCAGG ACAGCCCCGC CGCAGACGCC AGTGGACGGC CCAGCACCAG TGCCAGCCCT 
GCCGAGAGAA GCCCAGGCCC AAGCGTGAAT GGCCTGGAGG AGCCGAGCGT CGCCAAGAGA 
CTGCGGGGCA CCCCGGAGAG GATCGAGCTG GAGAACTACC GGCTGTCGGT GCGGCAGGCG 
GAGGGGCTGG AGGAGGTGCC CGAGGAGACG CAGGCCGAGC ACAACCTGAG CAGCGTGCTG 
GACACGGGCA CAGAGGAGGA CGCGGCCAGC AGCAGTTCGG AGTCGGAGAT GGAGGAAGAG 
GAGCCTCCGC TGCCCACCTC GGACCTGGGC GGCGTACCCT GGAAGGAGGC TGTGAGGATC 
CATGCGCTCC TGAGAGGGAA GAGTGAAGAG GAGCTGGAGG CCTCCAGGAG CTTCGGGGCC 
GGGGAGGAGG AGGAGGAAGA CGAGGAGGAC GAGGAGGAGG AGGAGGAGGA GGAAGACGAG 
GAGGACGAGG AGGAGGACGA GGATGAGTCT AGTGAAGTCG GGAGCCCAAG GAGGCTGCAG 
CAGCTGCTGA ACCCGGCCGA CCCCCTGGAG ATCCAGGCCG ACGTGCACTG GACGCATATC 
CGTGAGAGCC AGGAGGAGCG AGCGGCACTG GCGCCCAAGT CCCCCCTTCC CGGAGTGCCC 
GGCCTCCCCT CCGTCCGCCG CGCAGCAGTT CAGGCCTGGC TGGAGTCGGT CTCCGGAGTC 
CCATTCGATG AGGATGACCT GGAGCGAGAC GTGGACTCGG AACCAGCAGA GATCGAAGGG 
GAGGCAGCGG AGAATGGGGA CGCAGGGGAC ACTGGTGCCG AGCTGGACGA CGATCAGCAC 
TGGTCGGATG ACGTCCCGTC CGAGGCCAAC ACGGAGCTGC ACCTGCCGGC AGTGGGAGCA 
GAGCTGGAGC TGAGGGTGTC GGATGGCGAG GAGGAGCCAC CGCCGGCCTC AGTAGGGCAC 
CCAGAGAGAG GTCCCTCCCG AGTCTCCAGC CCCACCCGGT CCCCTGAGGA GCCCACGGGC 
CTGTCCTCCC CAGCCCGCAG CCCCGGGGCA CAGAGCGCCC ATCCCCCACT TGCCGCTGTG 
GCCACCGGGG TAAGGTCACC CGCCGAGAGC CCTCTGCCGG AGCCCTCGAC CCCGCCAGCG 
GAGCCCGAAG CTCACCCTCC CATCCGATCG CAGCCTGAAG CCAGGACGCC ACCCTCGCCT 
GCCAGCCCCC AGCGCCCATC CCCGCCCACC CAACTGCCCA TCTGCTCCCA GCCTCAGCCG 
TCCCCTGAGG CCACCGTACC GTCCCCCACC CTGTCCCCCA TCCGCTCCCA GCCCGTGCCG 
GCCAGAACCT CCACTCCTCT GGCCCCCCTC CCCGTGAAGA ACCAAGGGGT CACGAAGGAC 
ACACTGGGCA GCTCCCTCCC TGGGGACGAG GCCCTGAAAC GCAGCGACCT GGTGGCAGAG 
TTCTGGATGA AGAGTGCCGA GATCCGCCGC AGCCTGGGGC TCACGCCTGT GCACCGGAGC 
CCGGGGTCTG AGCTCGCCTT CCAGTCCCCG CCCCTGAAGG CCTGTCCAGC AGAGAAGGCC 
CCCCAGAGCG AGGGGCTCCG GCTCCTAAAA CCCCCACCTG TCCCCAGGAA GCTGGGGCTG 
CCAGCAGCCG AGGGGGCCCA GCCCTGCCCA CCCACCCCTG TGTCCCCGCC CGACAGGGAG 
CCAAAGGGCC CCCGGGAGGA GCACAGAGAC CTGTCCAGCA GCTCGGGGCT GGGCCTGCAG 
GGCAGCTCCT CCCGCACCAG GACACCGGGC AGCCAGAGCT TCAACACCTC TGACTCCACC 
ATGCTCACGC CTCCTTCCAG CCCGCCGCCC CCGCCGCCTG ACGAGGAGCC CGCTACCCTT 
CACAGGAAGC CAGCCCTGGC AGGGCAGCTT GTGGCCTCAG CACCCCCACC CCCGGCTGTA 
TGTGTGAGGC CCCCGCGGGA GCCCACCCAG CCCCCCCAGG AGGAGGCGCG GAAGTCTTTC 
GTGGAGAGCG TGGATGAGAT TCCCTTTGCG GATGATGTGG AGGACACGTA TGATGACAAC 
ACATGTGACG ACCGCACGGA GGACTCGAGT CTGCAGGAGA CCTTCTTCAC ACCCCCCTCC 
CACTGGCCCC ACCCCAAGCA GCCCTTGGCT CCGGAGAACG GCCGGGGGCC AGAGAGTGCG 
GTCCCACTGC AGAAGCGGGG GCTGCCGCTG GTCTCGGCCG AGGCCAAGGA GCTGGCGGCC 
GAGCGCATGC GGGCCCGGGA GAAGTCGGTG CGGAGCCAGG CACTGAGGGA CGCCATGGCC 
CGGCAGCTGA GCAGGATGAA GGAGATGGAC ATAGCAGCTG CAGCCCCCAG GACCCCCAGG 
ACCCCTGCAC CCCGCAGAGC CACTGCCGTG CCCCCCAAGG GCCCCGAGGA ACCTGCCCCG 
AGGCACGAAG CCACAAGTGA GGAGCTGCTG TCCCCTCCCT CGGACTCAGG GGGCCCGGAC 
GGCTCGGTCA CCTCGTCAGA GGGCTCCAGT GGGAAGAGCA AGAAGAGGTC GTCCCTCTTC 
TCTCCTCGGA GAAGCAAGAA AGAGAAGAAG CCCAAAGGCG AGGGCCGGCC CCTGGAGAGG 
CCCAGCCCCG GCACTCTGGA GGAAGCGGCC GCCAAGCCCA GGTCTCTGTG GAAGTCTGTC 
TTCTCGGGGT ACAGGAAGGA CAAGAAGAAG AGTGATGGCC GGTCTTGCCC CAGCACCCCG 
TCCAGCGGGA CCACAGTGGA CGCCGGCAAG CCCAGGGCAT CTCCCGTCTC AAGAGCAGAG 
CTGCGGACGC GGCGCCAGCT GAGCTGCTCG GAGGACTCGG ACCTCTCCAG TGACGACGTC 
CTCGAGCGGA CCTCCCAGAA GTCCAGAAAA GAGCCGAGAA CTTACACGGA GGAGGAGCTG 
AACGCCAAGC TGACCCGGCG TGTGCAGAAG GCAGCTCGGA GGCAGGCCAA GCAGGAGGAG 
CTGAAACGGC TGCACCGCGC GCAGATCATC CAGCGGCAGC TGGAGCAGGT GGAGGAGAAG 
CAGCGGCAGC TGGAGGAGAG GGGGGTCGCC GTGGAGAAGG CGCTGCGCGG GGAAGCAGTT 
GAGCCCAGTG GCGGGACTCC ACGGCGTAGA CCTCTCTCCT TCTGCCCTTG CTGTGTCCAG 
GAAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992780.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848548.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

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CloneID	OBa234964
Clone ID Related Accession (Same CDS sequence)	XM_024992780.1
Accession Version	XM_024992780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCGA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848548.1
CDS	1..6267
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992780.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992780.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCGA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992781.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848549.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

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CloneID	OBa234965
Clone ID Related Accession (Same CDS sequence)	XM_024992781.1
Accession Version	XM_024992781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CGAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGGCATG GGCAAGAAGG ATGACCCCAA GCTGATGCAG 
GAGTGGTTCA AGCTGGTGCA GGAGAAGAAC GCCATGGTGC GCTACGAGTC AGAGCTGATG 
ATCTTTGCTC GGGAGCTGGA GCTGGAGGAC CGGCAGAGCC GGCTGCAGCA GGAGCTCCGC 
GAGCGGATGG CGGTGGAAGA CCACCTGAAG ACGGAGGAGG AGCTGGCGGA GGAGAAGAGG 
ATCCTGAACG AGATGCTGGA GGTGGTGGAG CAGAGAGACG CCCTGGTGGC CCTGCTGGAG 
GAGCAGCGGT TGCGGGAGAA GGAGGAGGAC AAGGACCTGG AGGCCGCCAT GCTGTCCAAG 
GGCTTCAGCC TGCACTGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848549.1
CDS	1..6264
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992781.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992781.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CGAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGGCATG GGCAAGAAGG ATGACCCCAA GCTGATGCAG 
GAGTGGTTCA AGCTGGTGCA GGAGAAGAAC GCCATGGTGC GCTACGAGTC AGAGCTGATG 
ATCTTTGCTC GGGAGCTGGA GCTGGAGGAC CGGCAGAGCC GGCTGCAGCA GGAGCTCCGC 
GAGCGGATGG CGGTGGAAGA CCACCTGAAG ACGGAGGAGG AGCTGGCGGA GGAGAAGAGG 
ATCCTGAACG AGATGCTGGA GGTGGTGGAG CAGAGAGACG CCCTGGTGGC CCTGCTGGAG 
GAGCAGCGGT TGCGGGAGAA GGAGGAGGAC AAGGACCTGG AGGCCGCCAT GCTGTCCAAG 
GGCTTCAGCC TGCACTGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992782.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848550.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

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CloneID	OBa234966
Clone ID Related Accession (Same CDS sequence)	XM_024992782.1
Accession Version	XM_024992782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCGAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848550.1
CDS	1..6096
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992782.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992782.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCGAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_024992783.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848551.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

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CloneID	OBa234967
Clone ID Related Accession (Same CDS sequence)	XM_024992783.1
Accession Version	XM_024992783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL3 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCGAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848551.1
CDS	1..6012
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIEGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_024992783.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992783.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCCTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC GAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCGAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_005207355.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207412.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

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CloneID	OBa19955
Clone ID Related Accession (Same CDS sequence)	XM_005207355.3 , XM_015463593.1
Accession Version	XM_005207355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCAAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207412.1
CDS	244..6255
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_005207355.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207355.3

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCAAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463593.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319079.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

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CloneID	OBa19955
Clone ID Related Accession (Same CDS sequence)	XM_005207355.3 , XM_015463593.1
Accession Version	XM_015463593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCAAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319079.1
CDS	244..6255
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463593.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463593.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTGCCCG GCCTCCCCTC CGTCCGCCGC GCAGCAGTTC AGGCCTGGCT GGAGTCGGTC 
TCCGGAGTCC CATTCGATGA GGATGACCTG GAGCGAGACG TGGACTCGGA ACCAGCAGAG 
ATCAAAGGGG AGGCAGCGGA GAATGGGGAC GCAGGGGACA CTGGTGCCGA GCTGGACGAC 
GATCAGCACT GGTCGGATGA CGTCCCGTCC GAGGCCAACA CGGAGCTGCA CCTGCCGGCA 
GTGGGAGCAG AGCTGGAGCT GAGGGTGTCG GATGGCGAGG AGGAGCCACC GCCGGCCTCA 
GTAGGGCACC CAGAGAGAGG TCCCTCCCGA GTCTCCAGCC CCACCCGGTC CCCTGAGGAG 
CCCACGGGCC TGTCCTCCCC AGCCCGCAGC CCCGGGGCAC AGAGCGCCCA TCCCCCACTT 
GCCGCTGTGG CCACCGGGGT AAGGTCACCC GCCGAGAGCC CTCTGCCGGA GCCCTCGACC 
CCGCCAGCGG AGCCCGAAGC TCACCCTCCC ATCCGATCGC AGCCTGAAGC CAGGACGCCA 
CCCTCGCCTG CCAGCCCCCA GCGCCCATCC CCGCCCACCC AACTGCCCAT CTGCTCCCAG 
CCTCAGCCGT CCCCTGAGGC CACCGTACCG TCCCCCACCC TGTCCCCCAT CCGCTCCCAG 
CCCGTGCCGG CCAGAACCTC CACTCCTCTG GCCCCCCTCC CCGTGAAGAA CCAAGGGGTC 
ACGAAGGACA CACTGGGCAG CTCCCTCCCT GGGGACGAGG CCCTGAAACG CAGCGACCTG 
GTGGCAGAGT TCTGGATGAA GAGTGCCGAG ATCCGCCGCA GCCTGGGGCT CACGCCTGTG 
CACCGGAGCC CGGGGTCTGA GCTCGCCTTC CAGTCCCCGC CCCTGAAGGC CTGTCCAGCA 
GAGAAGGCCC CCCAGAGCGA GGGGCTCCGG CTCCTAAAAC CCCCACCTGT CCCCAGGAAG 
CTGGGGCTGC CAGCAGCCGA GGGGGCCCAG CCCTGCCCAC CCACCCCTGT GTCCCCGCCC 
GACAGGGAGC CAAAGGGCCC CCGGGAGGAG CACAGAGACC TGTCCAGCAG CTCGGGGCTG 
GGCCTGCAGG GCAGCTCCTC CCGCACCAGG ACACCGGGCA GCCAGAGCTT CAACACCTCT 
GACTCCACCA TGCTCACGCC TCCTTCCAGC CCGCCGCCCC CGCCGCCTGA CGAGGAGCCC 
GCTACCCTTC ACAGGAAGCC AGCCCTGGCA GGGCAGCTTG TGGCCTCAGC ACCCCCACCC 
CCGGCTGTAT GTGTGAGGCC CCCGCGGGAG CCCACCCAGC CCCCCCAGGA GGAGGCGCGG 
AAGTCTTTCG TGGAGAGCGT GGATGAGATT CCCTTTGCGG ATGATGTGGA GGACACGTAT 
GATGACAACA CATGTGACGA CCGCACGGAG GACTCGAGTC TGCAGGAGAC CTTCTTCACA 
CCCCCCTCCC ACTGGCCCCA CCCCAAGCAG CCCTTGGCTC CGGAGAACGG CCGGGGGCCA 
GAGAGTGCGG TCCCACTGCA GAAGCGGGGG CTGCCGCTGG TCTCGGCCGA GGCCAAGGAG 
CTGGCGGCCG AGCGCATGCG GGCCCGGGAG AAGTCGGTGC GGAGCCAGGC ACTGAGGGAC 
GCCATGGCCC GGCAGCTGAG CAGGATGAAG GAGATGGACA TAGCAGCTGC AGCCCCCAGG 
ACCCCCAGGA CCCCTGCACC CCGCAGAGCC ACTGCCGTGC CCCCCAAGGG CCCCGAGGAA 
CCTGCCCCGA GGCACGAAGC CACAAGTGAG GAGCTGCTGT CCCCTCCCTC GGACTCAGGG 
GGCCCGGACG GCTCGGTCAC CTCGTCAGAG GGCTCCAGTG GGAAGAGCAA GAAGAGGTCG 
TCCCTCTTCT CTCCTCGGAG AAGCAAGAAA GAGAAGAAGC CCAAAGGCGA GGGCCGGCCC 
CTGGAGAGGC CCAGCCCCGG CACTCTGGAG GAAGCGGCCG CCAAGCCCAG GTCTCTGTGG 
AAGTCTGTCT TCTCGGGGTA CAGGAAGGAC AAGAAGAAGA GTGATGGCCG GTCTTGCCCC 
AGCACCCCGT CCAGCGGGAC CACAGTGGAC GCCGGCAAGC CCAGGGCATC TCCCGTCTCA 
AGAGCAGAGC TGCGGACGCG GCGCCAGCTG AGCTGCTCGG AGGACTCGGA CCTCTCCAGT 
GACGACGTCC TCGAGCGGAC CTCCCAGAAG TCCAGAAAAG AGCCGAGAAC TTACACGGAG 
GAGGAGCTGA ACGCCAAGCT GACCCGGCGT GTGCAGAAGG CAGCTCGGAG GCAGGCCAAG 
CAGGAGGAGC TGAAACGGCT GCACCGCGCG CAGATCATCC AGCGGCAGCT GGAGCAGGTG 
GAGGAGAAGC AGCGGCAGCT GGAGGAGAGG GGGGTCGCCG TGGAGAAGGC GCTGCGCGGG 
GAAGCAGTTG AGCCCAGTGG CGGGACTCCA CGGCGTAGAC CTCTCTCCTT CTGCCCTTGC 
TGTGTCCAGG AAGGCATGGG CAAGAAGGAT GACCCCAAGC TGATGCAGGA GTGGTTCAAG 
CTGGTGCAGG AGAAGAACGC CATGGTGCGC TACGAGTCAG AGCTGATGAT CTTTGCTCGG 
GAGCTGGAGC TGGAGGACCG GCAGAGCCGG CTGCAGCAGG AGCTCCGCGA GCGGATGGCG 
GTGGAAGACC ACCTGAAGAC GGAGGAGGAG CTGGCGGAGG AGAAGAGGAT CCTGAACGAG 
ATGCTGGAGG TGGTGGAGCA GAGAGACGCC CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG 
CGGGAGAAGG AGGAGGACAA GGACCTGGAG GCCGCCATGC TGTCCAAGGG CTTCAGCCTG 
CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_005207361.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207418.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

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CloneID	OBa19958
Clone ID Related Accession (Same CDS sequence)	XM_005207361.3 , XM_015463594.1
Accession Version	XM_005207361.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGTTGAGC CCAGTGGCGG GACTCCACGG CGTAGACCTC TCTCCTTCTG CCCTTGCTGT 
GTCCAGGAAG GCATGGGCAA GAAGGATGAC CCCAAGCTGA TGCAGGAGTG GTTCAAGCTG 
GTGCAGGAGA AGAACGCCAT GGTGCGCTAC GAGTCAGAGC TGATGATCTT TGCTCGGGAG 
CTGGAGCTGG AGGACCGGCA GAGCCGGCTG CAGCAGGAGC TCCGCGAGCG GATGGCGGTG 
GAAGACCACC TGAAGACGGA GGAGGAGCTG GCGGAGGAGA AGAGGATCCT GAACGAGATG 
CTGGAGGTGG TGGAGCAGAG AGACGCCCTG GTGGCCCTGC TGGAGGAGCA GCGGTTGCGG 
GAGAAGGAGG AGGACAAGGA CCTGGAGGCC GCCATGCTGT CCAAGGGCTT CAGCCTGCAC 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207418.1
CDS	244..6192
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_005207361.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207361.3

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGTTGAGC CCAGTGGCGG GACTCCACGG CGTAGACCTC TCTCCTTCTG CCCTTGCTGT 
GTCCAGGAAG GCATGGGCAA GAAGGATGAC CCCAAGCTGA TGCAGGAGTG GTTCAAGCTG 
GTGCAGGAGA AGAACGCCAT GGTGCGCTAC GAGTCAGAGC TGATGATCTT TGCTCGGGAG 
CTGGAGCTGG AGGACCGGCA GAGCCGGCTG CAGCAGGAGC TCCGCGAGCG GATGGCGGTG 
GAAGACCACC TGAAGACGGA GGAGGAGCTG GCGGAGGAGA AGAGGATCCT GAACGAGATG 
CTGGAGGTGG TGGAGCAGAG AGACGCCCTG GTGGCCCTGC TGGAGGAGCA GCGGTTGCGG 
GAGAAGGAGG AGGACAAGGA CCTGGAGGCC GCCATGCTGT CCAAGGGCTT CAGCCTGCAC 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463594.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319080.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

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CloneID	OBa19958
Clone ID Related Accession (Same CDS sequence)	XM_005207361.3 , XM_015463594.1
Accession Version	XM_015463594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGTTGAGC CCAGTGGCGG GACTCCACGG CGTAGACCTC TCTCCTTCTG CCCTTGCTGT 
GTCCAGGAAG GCATGGGCAA GAAGGATGAC CCCAAGCTGA TGCAGGAGTG GTTCAAGCTG 
GTGCAGGAGA AGAACGCCAT GGTGCGCTAC GAGTCAGAGC TGATGATCTT TGCTCGGGAG 
CTGGAGCTGG AGGACCGGCA GAGCCGGCTG CAGCAGGAGC TCCGCGAGCG GATGGCGGTG 
GAAGACCACC TGAAGACGGA GGAGGAGCTG GCGGAGGAGA AGAGGATCCT GAACGAGATG 
CTGGAGGTGG TGGAGCAGAG AGACGCCCTG GTGGCCCTGC TGGAGGAGCA GCGGTTGCGG 
GAGAAGGAGG AGGACAAGGA CCTGGAGGCC GCCATGCTGT CCAAGGGCTT CAGCCTGCAC 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319080.1
CDS	244..6192
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463594.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463594.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGTTGAGC CCAGTGGCGG GACTCCACGG CGTAGACCTC TCTCCTTCTG CCCTTGCTGT 
GTCCAGGAAG GCATGGGCAA GAAGGATGAC CCCAAGCTGA TGCAGGAGTG GTTCAAGCTG 
GTGCAGGAGA AGAACGCCAT GGTGCGCTAC GAGTCAGAGC TGATGATCTT TGCTCGGGAG 
CTGGAGCTGG AGGACCGGCA GAGCCGGCTG CAGCAGGAGC TCCGCGAGCG GATGGCGGTG 
GAAGACCACC TGAAGACGGA GGAGGAGCTG GCGGAGGAGA AGAGGATCCT GAACGAGATG 
CTGGAGGTGG TGGAGCAGAG AGACGCCCTG GTGGCCCTGC TGGAGGAGCA GCGGTTGCGG 
GAGAAGGAGG AGGACAAGGA CCTGGAGGCC GCCATGCTGT CCAAGGGCTT CAGCCTGCAC 
TGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319081.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

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CloneID	OBa19960
Clone ID Related Accession (Same CDS sequence)	XM_015463595.1 , XM_002687919.5
Accession Version	XM_015463595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319081.1
CDS	243..6125
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463595.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_002687919.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002687965.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

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CloneID	OBa19960
Clone ID Related Accession (Same CDS sequence)	XM_015463595.1 , XM_002687919.5
Accession Version	XM_002687919.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5883bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687919.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002687965.2
CDS	243..6125
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_002687919.5
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002687919.5

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GGGCTCCATA AAGAAGGAGT TCCCGCAGAA CTTGGGAGGC 
AGCGACACCT GCTATTTCTG CCAGAAGCGG GTCTATGTGA TGGAGCGGCT GAGCGCCGAG 
GGCAAGTTCT TCCACCGGAG CTGCTTCAAG TGCGAGCACT GCGCCACCAC CCTGCGCCTG 
TCGGCCTATG CCTACGCCCT CGAGGACGGT AAATTCTACT GCAAGCCACA CTACTGTTAC 
CGGCTGTCTG GCCCTGCACA GAGGAAGAGA CCGGCAGGGG TGCCTCTGTC TGGAAAGGAG 
GCCCGGGGAC CCCTGCAGGA CAGCCCCGCC GCAGACGCCA GTGGACGGCC CAGCACCAGT 
GCCAGCCCTG CCGAGAGAAG CCCAGGCCCA AGCGTGAATG GCCTGGAGGA GCCGAGCGTC 
GCCAAGAGAC TGCGGGGCAC CCCGGAGAGG ATCGAGCTGG AGAACTACCG GCTGTCGGTG 
CGGCAGGCGG AGGGGCTGGA GGAGGTGCCC GAGGAGACGC AGGCCGAGCA CAACCTGAGC 
AGCGTGCTGG ACACGGGCAC AGAGGAGGAC GCGGCCAGCA GCAGTTCGGA GTCGGAGATG 
GAGGAAGAGG AGCCTCCGCT GCCCACCTCG GACCTGGGCG GCGTACCCTG GAAGGAGGCT 
GTGAGGATCC ATGCGCTCCT GAGAGGGAAG AGTGAAGAGG AGCTGGAGGC CTCCAGGAGC 
TTCGGGGCCG GGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGACGAG GATGAGTCTA GTGAAGTCGG GAGCCCAAGG 
AGGCTGCAGC AGCTGCTGAA CCCGGCCGAC CCCCTGGAGA TCCAGGCCGA CGTGCACTGG 
ACGCATATCC GTGAGAGCCA GGAGGAGCGA GCGGCACTGG CGCCCAAGTC CCCCCTTCCC 
GGAGTCCCAT TCGATGAGGA TGACCTGGAG CGAGACGTGG ACTCGGAACC AGCAGAGATC 
AAAGGGGAGG CAGCGGAGAA TGGGGACGCA GGGGACACTG GTGCCGAGCT GGACGACGAT 
CAGCACTGGT CGGATGACGT CCCGTCCGAG GCCAACACGG AGCTGCACCT GCCGGCAGTG 
GGAGCAGAGC TGGAGCTGAG GGTGTCGGAT GGCGAGGAGG AGCCACCGCC GGCCTCAGTA 
GGGCACCCAG AGAGAGGTCC CTCCCGAGTC TCCAGCCCCA CCCGGTCCCC TGAGGAGCCC 
ACGGGCCTGT CCTCCCCAGC CCGCAGCCCC GGGGCACAGA GCGCCCATCC CCCACTTGCC 
GCTGTGGCCA CCGGGGTAAG GTCACCCGCC GAGAGCCCTC TGCCGGAGCC CTCGACCCCG 
CCAGCGGAGC CCGAAGCTCA CCCTCCCATC CGATCGCAGC CTGAAGCCAG GACGCCACCC 
TCGCCTGCCA GCCCCCAGCG CCCATCCCCG CCCACCCAAC TGCCCATCTG CTCCCAGCCT 
CAGCCGTCCC CTGAGGCCAC CGTACCGTCC CCCACCCTGT CCCCCATCCG CTCCCAGCCC 
GTGCCGGCCA GAACCTCCAC TCCTCTGGCC CCCCTCCCCG TGAAGAACCA AGGGGTCACG 
AAGGACACAC TGGGCAGCTC CCTCCCTGGG GACGAGGCCC TGAAACGCAG CGACCTGGTG 
GCAGAGTTCT GGATGAAGAG TGCCGAGATC CGCCGCAGCC TGGGGCTCAC GCCTGTGCAC 
CGGAGCCCGG GGTCTGAGCT CGCCTTCCAG TCCCCGCCCC TGAAGGCCTG TCCAGCAGAG 
AAGGCCCCCC AGAGCGAGGG GCTCCGGCTC CTAAAACCCC CACCTGTCCC CAGGAAGCTG 
GGGCTGCCAG CAGCCGAGGG GGCCCAGCCC TGCCCACCCA CCCCTGTGTC CCCGCCCGAC 
AGGGAGCCAA AGGGCCCCCG GGAGGAGCAC AGAGACCTGT CCAGCAGCTC GGGGCTGGGC 
CTGCAGGGCA GCTCCTCCCG CACCAGGACA CCGGGCAGCC AGAGCTTCAA CACCTCTGAC 
TCCACCATGC TCACGCCTCC TTCCAGCCCG CCGCCCCCGC CGCCTGACGA GGAGCCCGCT 
ACCCTTCACA GGAAGCCAGC CCTGGCAGGG CAGCTTGTGG CCTCAGCACC CCCACCCCCG 
GCTGTATGTG TGAGGCCCCC GCGGGAGCCC ACCCAGCCCC CCCAGGAGGA GGCGCGGAAG 
TCTTTCGTGG AGAGCGTGGA TGAGATTCCC TTTGCGGATG ATGTGGAGGA CACGTATGAT 
GACAACACAT GTGACGACCG CACGGAGGAC TCGAGTCTGC AGGAGACCTT CTTCACACCC 
CCCTCCCACT GGCCCCACCC CAAGCAGCCC TTGGCTCCGG AGAACGGCCG GGGGCCAGAG 
AGTGCGGTCC CACTGCAGAA GCGGGGGCTG CCGCTGGTCT CGGCCGAGGC CAAGGAGCTG 
GCGGCCGAGC GCATGCGGGC CCGGGAGAAG TCGGTGCGGA GCCAGGCACT GAGGGACGCC 
ATGGCCCGGC AGCTGAGCAG GATGAAGGAG ATGGACATAG CAGCTGCAGC CCCCAGGACC 
CCCAGGACCC CTGCACCCCG CAGAGCCACT GCCGTGCCCC CCAAGGGCCC CGAGGAACCT 
GCCCCGAGGC ACGAAGCCAC AAGTGAGGAG CTGCTGTCCC CTCCCTCGGA CTCAGGGGGC 
CCGGACGGCT CGGTCACCTC GTCAGAGGGC TCCAGTGGGA AGAGCAAGAA GAGGTCGTCC 
CTCTTCTCTC CTCGGAGAAG CAAGAAAGAG AAGAAGCCCA AAGGCGAGGG CCGGCCCCTG 
GAGAGGCCCA GCCCCGGCAC TCTGGAGGAA GCGGCCGCCA AGCCCAGGTC TCTGTGGAAG 
TCTGTCTTCT CGGGGTACAG GAAGGACAAG AAGAAGAGTG ATGGCCGGTC TTGCCCCAGC 
ACCCCGTCCA GCGGGACCAC AGTGGACGCC GGCAAGCCCA GGGCATCTCC CGTCTCAAGA 
GCAGAGCTGC GGACGCGGCG CCAGCTGAGC TGCTCGGAGG ACTCGGACCT CTCCAGTGAC 
GACGTCCTCG AGCGGACCTC CCAGAAGTCC AGAAAAGAGC CGAGAACTTA CACGGAGGAG 
GAGCTGAACG CCAAGCTGAC CCGGCGTGTG CAGAAGGCAG CTCGGAGGCA GGCCAAGCAG 
GAGGAGCTGA AACGGCTGCA CCGCGCGCAG ATCATCCAGC GGCAGCTGGA GCAGGTGGAG 
GAGAAGCAGC GGCAGCTGGA GGAGAGGGGG GTCGCCGTGG AGAAGGCGCT GCGCGGGGAA 
GCAGGCATGG GCAAGAAGGA TGACCCCAAG CTGATGCAGG AGTGGTTCAA GCTGGTGCAG 
GAGAAGAACG CCATGGTGCG CTACGAGTCA GAGCTGATGA TCTTTGCTCG GGAGCTGGAG 
CTGGAGGACC GGCAGAGCCG GCTGCAGCAG GAGCTCCGCG AGCGGATGGC GGTGGAAGAC 
CACCTGAAGA CGGAGGAGGA GCTGGCGGAG GAGAAGAGGA TCCTGAACGA GATGCTGGAG 
GTGGTGGAGC AGAGAGACGC CCTGGTGGCC CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG 
GAGGAGGACA AGGACCTGGA GGCCGCCATG CTGTCCAAGG GCTTCAGCCT GCACTGGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805721.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804023.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_010805721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804023.1
CDS	434..6901
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805721.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805721.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015471384.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326870.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_015471384.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326870.1
CDS	455..6922
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015471384.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471384.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463583.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319069.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_015463583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319069.1
CDS	246..6713
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463583.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463583.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463584.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319070.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_015463584.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319070.1
CDS	434..6901
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463584.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463584.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805720.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804022.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_010805720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804022.1
CDS	246..6713
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805720.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805720.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463585.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319071.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

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CloneID	OBa52131
Clone ID Related Accession (Same CDS sequence)	XM_010805721.2 , XM_015471384.1 , XM_015463583.1 , XM_015463584.1 , XM_010805720.2 , XM_015463585.1
Accession Version	XM_015463585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319071.1
CDS	455..6922
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463585.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463585.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGTTGAGCC CAGTGGCGGG 
ACTCCACGGC GTAGACCTCT CTCCTTCTGC CCTTGCTGTG TCCAGGAAGG CATGGGCAAG 
AAGGATGACC CCAAGCTGAT GCAGGAGTGG TTCAAGCTGG TGCAGGAGAA GAACGCCATG 
GTGCGCTACG AGTCAGAGCT GATGATCTTT GCTCGGGAGC TGGAGCTGGA GGACCGGCAG 
AGCCGGCTGC AGCAGGAGCT CCGCGAGCGG ATGGCGGTGG AAGACCACCT GAAGACGGAG 
GAGGAGCTGG CGGAGGAGAA GAGGATCCTG AACGAGATGC TGGAGGTGGT GGAGCAGAGA 
GACGCCCTGG TGGCCCTGCT GGAGGAGCAG CGGTTGCGGG AGAAGGAGGA GGACAAGGAC 
CTGGAGGCCG CCATGCTGTC CAAGGGCTTC AGCCTGCACT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805722.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804024.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

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CloneID	OBa52132
Clone ID Related Accession (Same CDS sequence)	XM_010805722.2 , XM_015463586.1
Accession Version	XM_010805722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804024.1
CDS	246..6710
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805722.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805722.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463586.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319072.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

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CloneID	OBa52132
Clone ID Related Accession (Same CDS sequence)	XM_010805722.2 , XM_015463586.1
Accession Version	XM_015463586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319072.1
CDS	246..6710
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463586.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463586.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGTTC GGAGTCGGAG ATGGAGGAAG AGGAGCCTCC GCTGCCCACC 
TCGGACCTGG GCGGCGTACC CTGGAAGGAG GCTGTGAGGA TCCATGCGCT CCTGAGAGGG 
AAGAGTGAAG AGGAGCTGGA GGCCTCCAGG AGCTTCGGGG CCGGGGAGGA GGAGGAGGAA 
GACGAGGAGG ACGAGGAGGA GGAGGAGGAG GAGGAAGACG AGGAGGACGA GGAGGAGGAC 
GAGGATGAGT CTAGTGAAGT CGGGAGCCCA AGGAGGCTGC AGCAGCTGCT GAACCCGGCC 
GACCCCCTGG AGATCCAGGC CGACGTGCAC TGGACGCATA TCCGTGAGAG CCAGGAGGAG 
CGAGCGGCAC TGGCGCCCAA GTCCCCCCTT CCCGGAGTGC CCGGCCTCCC CTCCGTCCGC 
CGCGCAGCAG TTCAGGCCTG GCTGGAGTCG GTCTCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463587.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319073.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

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CloneID	OBa52133
Clone ID Related Accession (Same CDS sequence)	XM_015463587.1 , XM_010805723.2
Accession Version	XM_015463587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319073.1
CDS	246..6698
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYTDLIDLHLGGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463587.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805723.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804025.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

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CloneID	OBa52133
Clone ID Related Accession (Same CDS sequence)	XM_015463587.1 , XM_010805723.2
Accession Version	XM_010805723.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805723.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804025.1
CDS	246..6698
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEADYWGESYYTDLIDLHLGGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805723.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805723.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGACTATTG GGGAGAGTCT 
TATTACACTG ACCTCATCGA CTTGCATCTG GGTGGCATGG GCAAGAAGGA TGACCCCAAG 
CTGATGCAGG AGTGGTTCAA GCTGGTGCAG GAGAAGAACG CCATGGTGCG CTACGAGTCA 
GAGCTGATGA TCTTTGCTCG GGAGCTGGAG CTGGAGGACC GGCAGAGCCG GCTGCAGCAG 
GAGCTCCGCG AGCGGATGGC GGTGGAAGAC CACCTGAAGA CGGAGGAGGA GCTGGCGGAG 
GAGAAGAGGA TCCTGAACGA GATGCTGGAG GTGGTGGAGC AGAGAGACGC CCTGGTGGCC 
CTGCTGGAGG AGCAGCGGTT GCGGGAGAAG GAGGAGGACA AGGACCTGGA GGCCGCCATG 
CTGTCCAAGG GCTTCAGCCT GCACTGGTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805724.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804026.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

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CloneID	OBa52134
Clone ID Related Accession (Same CDS sequence)	XM_010805724.2 , XM_015463588.1
Accession Version	XM_010805724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805724.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804026.1
CDS	246..6650
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805724.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805724.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463588.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319074.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

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CloneID	OBa52134
Clone ID Related Accession (Same CDS sequence)	XM_010805724.2 , XM_015463588.1
Accession Version	XM_015463588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319074.1
CDS	246..6650
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463588.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463588.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TCCCATTCGA TGAGGATGAC 
CTGGAGCGAG ACGTGGACTC GGAACCAGCA GAGATCAAAG GGGAGGCAGC GGAGAATGGG 
GACGCAGGGG ACACTGGTGC CGAGCTGGAC GACGATCAGC ACTGGTCGGA TGACGTCCCG 
TCCGAGGCCA ACACGGAGCT GCACCTGCCG GCAGTGGGAG CAGAGCTGGA GCTGAGGGTG 
TCGGATGGCG AGGAGGAGCC ACCGCCGGCC TCAGTAGGGC ACCCAGAGAG AGGTCCCTCC 
CGAGTCTCCA GCCCCACCCG GTCCCCTGAG GAGCCCACGG GCCTGTCCTC CCCAGCCCGC 
AGCCCCGGGG CACAGAGCGC CCATCCCCCA CTTGCCGCTG TGGCCACCGG GGTAAGGTCA 
CCCGCCGAGA GCCCTCTGCC GGAGCCCTCG ACCCCGCCAG CGGAGCCCGA AGCTCACCCT 
CCCATCCGAT CGCAGCCTGA AGCCAGGACG CCACCCTCGC CTGCCAGCCC CCAGCGCCCA 
TCCCCGCCCA CCCAACTGCC CATCTGCTCC CAGCCTCAGC CGTCCCCTGA GGCCACCGTA 
CCGTCCCCCA CCCTGTCCCC CATCCGCTCC CAGCCCGTGC CGGCCAGAAC CTCCACTCCT 
CTGGCCCCCC TCCCCGTGAA GAACCAAGGG GTCACGAAGG ACACACTGGG CAGCTCCCTC 
CCTGGGGACG AGGCCCTGAA ACGCAGCGAC CTGGTGGCAG AGTTCTGGAT GAAGAGTGCC 
GAGATCCGCC GCAGCCTGGG GCTCACGCCT GTGCACCGGA GCCCGGGGTC TGAGCTCGCC 
TTCCAGTCCC CGCCCCTGAA GGCCTGTCCA GCAGAGAAGG CCCCCCAGAG CGAGGGGCTC 
CGGCTCCTAA AACCCCCACC TGTCCCCAGG AAGCTGGGGC TGCCAGCAGC CGAGGGGGCC 
CAGCCCTGCC CACCCACCCC TGTGTCCCCG CCCGACAGGG AGCCAAAGGG CCCCCGGGAG 
GAGCACAGAG ACCTGTCCAG CAGCTCGGGG CTGGGCCTGC AGGGCAGCTC CTCCCGCACC 
AGGACACCGG GCAGCCAGAG CTTCAACACC TCTGACTCCA CCATGCTCAC GCCTCCTTCC 
AGCCCGCCGC CCCCGCCGCC TGACGAGGAG CCCGCTACCC TTCACAGGAA GCCAGCCCTG 
GCAGGGCAGC TTGTGGCCTC AGCACCCCCA CCCCCGGCTG TATGTGTGAG GCCCCCGCGG 
GAGCCCACCC AGCCCCCCCA GGAGGAGGCG CGGAAGTCTT TCGTGGAGAG CGTGGATGAG 
ATTCCCTTTG CGGATGATGT GGAGGACACG TATGATGACA ACACATGTGA CGACCGCACG 
GAGGACTCGA GTCTGCAGGA GACCTTCTTC ACACCCCCCT CCCACTGGCC CCACCCCAAG 
CAGCCCTTGG CTCCGGAGAA CGGCCGGGGG CCAGAGAGTG CGGTCCCACT GCAGAAGCGG 
GGGCTGCCGC TGGTCTCGGC CGAGGCCAAG GAGCTGGCGG CCGAGCGCAT GCGGGCCCGG 
GAGAAGTCGG TGCGGAGCCA GGCACTGAGG GACGCCATGG CCCGGCAGCT GAGCAGGATG 
AAGGAGATGG ACATAGCAGC TGCAGCCCCC AGGACCCCCA GGACCCCTGC ACCCCGCAGA 
GCCACTGCCG TGCCCCCCAA GGGCCCCGAG GAACCTGCCC CGAGGCACGA AGCCACAAGT 
GAGGAGCTGC TGTCCCCTCC CTCGGACTCA GGGGGCCCGG ACGGCTCGGT CACCTCGTCA 
GAGGGCTCCA GTGGGAAGAG CAAGAAGAGG TCGTCCCTCT TCTCTCCTCG GAGAAGCAAG 
AAAGAGAAGA AGCCCAAAGG CGAGGGCCGG CCCCTGGAGA GGCCCAGCCC CGGCACTCTG 
GAGGAAGCGG CCGCCAAGCC CAGGTCTCTG TGGAAGTCTG TCTTCTCGGG GTACAGGAAG 
GACAAGAAGA AGAGTGATGG CCGGTCTTGC CCCAGCACCC CGTCCAGCGG GACCACAGTG 
GACGCCGGCA AGCCCAGGGC ATCTCCCGTC TCAAGAGCAG AGCTGCGGAC GCGGCGCCAG 
CTGAGCTGCT CGGAGGACTC GGACCTCTCC AGTGACGACG TCCTCGAGCG GACCTCCCAG 
AAGTCCAGAA AAGAGCCGAG AACTTACACG GAGGAGGAGC TGAACGCCAA GCTGACCCGG 
CGTGTGCAGA AGGCAGCTCG GAGGCAGGCC AAGCAGGAGG AGCTGAAACG GCTGCACCGC 
GCGCAGATCA TCCAGCGGCA GCTGGAGCAG GTGGAGGAGA AGCAGCGGCA GCTGGAGGAG 
AGGGGGGTCG CCGTGGAGAA GGCGCTGCGC GGGGAAGCAG TTGAGCCCAG TGGCGGGACT 
CCACGGCGTA GACCTCTCTC CTTCTGCCCT TGCTGTGTCC AGGAAGGCAT GGGCAAGAAG 
GATGACCCCA AGCTGATGCA GGAGTGGTTC AAGCTGGTGC AGGAGAAGAA CGCCATGGTG 
CGCTACGAGT CAGAGCTGAT GATCTTTGCT CGGGAGCTGG AGCTGGAGGA CCGGCAGAGC 
CGGCTGCAGC AGGAGCTCCG CGAGCGGATG GCGGTGGAAG ACCACCTGAA GACGGAGGAG 
GAGCTGGCGG AGGAGAAGAG GATCCTGAAC GAGATGCTGG AGGTGGTGGA GCAGAGAGAC 
GCCCTGGTGG CCCTGCTGGA GGAGCAGCGG TTGCGGGAGA AGGAGGAGGA CAAGGACCTG 
GAGGCCGCCA TGCTGTCCAA GGGCTTCAGC CTGCACTGGT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010827969.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010826271.2 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

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CloneID	OBa52135
Clone ID Related Accession (Same CDS sequence)	XM_010827969.2 , XM_010805725.2
Accession Version	XM_010827969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010827969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010826271.2
CDS	245..6646
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010827969.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010827969.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805725.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804027.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

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CloneID	OBa52135
Clone ID Related Accession (Same CDS sequence)	XM_010827969.2 , XM_010805725.2
Accession Version	XM_010805725.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805725.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804027.1
CDS	245..6646
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805725.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805725.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCGGGAGC CCAAGGAGGC TGCAGCAGCT GCTGAACCCG 
GCCGACCCCC TGGAGATCCA GGCCGACGTG CACTGGACGC ATATCCGTGA GAGCCAGGAG 
GAGCGAGCGG CACTGGCGCC CAAGTCCCCC CTTCCCGGAG TGCCCGGCCT CCCCTCCGTC 
CGCCGCGCAG CAGTTCAGGC CTGGCTGGAG TCGGTCTCCG GAGTCCCATT CGATGAGGAT 
GACCTGGAGC GAGACGTGGA CTCGGAACCA GCAGAGATCA AAGGGGAGGC AGCGGAGAAT 
GGGGACGCAG GGGACACTGG TGCCGAGCTG GACGACGATC AGCACTGGTC GGATGACGTC 
CCGTCCGAGG CCAACACGGA GCTGCACCTG CCGGCAGTGG GAGCAGAGCT GGAGCTGAGG 
GTGTCGGATG GCGAGGAGGA GCCACCGCCG GCCTCAGTAG GGCACCCAGA GAGAGGTCCC 
TCCCGAGTCT CCAGCCCCAC CCGGTCCCCT GAGGAGCCCA CGGGCCTGTC CTCCCCAGCC 
CGCAGCCCCG GGGCACAGAG CGCCCATCCC CCACTTGCCG CTGTGGCCAC CGGGGTAAGG 
TCACCCGCCG AGAGCCCTCT GCCGGAGCCC TCGACCCCGC CAGCGGAGCC CGAAGCTCAC 
CCTCCCATCC GATCGCAGCC TGAAGCCAGG ACGCCACCCT CGCCTGCCAG CCCCCAGCGC 
CCATCCCCGC CCACCCAACT GCCCATCTGC TCCCAGCCTC AGCCGTCCCC TGAGGCCACC 
GTACCGTCCC CCACCCTGTC CCCCATCCGC TCCCAGCCCG TGCCGGCCAG AACCTCCACT 
CCTCTGGCCC CCCTCCCCGT GAAGAACCAA GGGGTCACGA AGGACACACT GGGCAGCTCC 
CTCCCTGGGG ACGAGGCCCT GAAACGCAGC GACCTGGTGG CAGAGTTCTG GATGAAGAGT 
GCCGAGATCC GCCGCAGCCT GGGGCTCACG CCTGTGCACC GGAGCCCGGG GTCTGAGCTC 
GCCTTCCAGT CCCCGCCCCT GAAGGCCTGT CCAGCAGAGA AGGCCCCCCA GAGCGAGGGG 
CTCCGGCTCC TAAAACCCCC ACCTGTCCCC AGGAAGCTGG GGCTGCCAGC AGCCGAGGGG 
GCCCAGCCCT GCCCACCCAC CCCTGTGTCC CCGCCCGACA GGGAGCCAAA GGGCCCCCGG 
GAGGAGCACA GAGACCTGTC CAGCAGCTCG GGGCTGGGCC TGCAGGGCAG CTCCTCCCGC 
ACCAGGACAC CGGGCAGCCA GAGCTTCAAC ACCTCTGACT CCACCATGCT CACGCCTCCT 
TCCAGCCCGC CGCCCCCGCC GCCTGACGAG GAGCCCGCTA CCCTTCACAG GAAGCCAGCC 
CTGGCAGGGC AGCTTGTGGC CTCAGCACCC CCACCCCCGG CTGTATGTGT GAGGCCCCCG 
CGGGAGCCCA CCCAGCCCCC CCAGGAGGAG GCGCGGAAGT CTTTCGTGGA GAGCGTGGAT 
GAGATTCCCT TTGCGGATGA TGTGGAGGAC ACGTATGATG ACAACACATG TGACGACCGC 
ACGGAGGACT CGAGTCTGCA GGAGACCTTC TTCACACCCC CCTCCCACTG GCCCCACCCC 
AAGCAGCCCT TGGCTCCGGA GAACGGCCGG GGGCCAGAGA GTGCGGTCCC ACTGCAGAAG 
CGGGGGCTGC CGCTGGTCTC GGCCGAGGCC AAGGAGCTGG CGGCCGAGCG CATGCGGGCC 
CGGGAGAAGT CGGTGCGGAG CCAGGCACTG AGGGACGCCA TGGCCCGGCA GCTGAGCAGG 
ATGAAGGAGA TGGACATAGC AGCTGCAGCC CCCAGGACCC CCAGGACCCC TGCACCCCGC 
AGAGCCACTG CCGTGCCCCC CAAGGGCCCC GAGGAACCTG CCCCGAGGCA CGAAGCCACA 
AGTGAGGAGC TGCTGTCCCC TCCCTCGGAC TCAGGGGGCC CGGACGGCTC GGTCACCTCG 
TCAGAGGGCT CCAGTGGGAA GAGCAAGAAG AGGTCGTCCC TCTTCTCTCC TCGGAGAAGC 
AAGAAAGAGA AGAAGCCCAA AGGCGAGGGC CGGCCCCTGG AGAGGCCCAG CCCCGGCACT 
CTGGAGGAAG CGGCCGCCAA GCCCAGGTCT CTGTGGAAGT CTGTCTTCTC GGGGTACAGG 
AAGGACAAGA AGAAGAGTGA TGGCCGGTCT TGCCCCAGCA CCCCGTCCAG CGGGACCACA 
GTGGACGCCG GCAAGCCCAG GGCATCTCCC GTCTCAAGAG CAGAGCTGCG GACGCGGCGC 
CAGCTGAGCT GCTCGGAGGA CTCGGACCTC TCCAGTGACG ACGTCCTCGA GCGGACCTCC 
CAGAAGTCCA GAAAAGAGCC GAGAACTTAC ACGGAGGAGG AGCTGAACGC CAAGCTGACC 
CGGCGTGTGC AGAAGGCAGC TCGGAGGCAG GCCAAGCAGG AGGAGCTGAA ACGGCTGCAC 
CGCGCGCAGA TCATCCAGCG GCAGCTGGAG CAGGTGGAGG AGAAGCAGCG GCAGCTGGAG 
GAGAGGGGGG TCGCCGTGGA GAAGGCGCTG CGCGGGGAAG CAGGCATGGG CAAGAAGGAT 
GACCCCAAGC TGATGCAGGA GTGGTTCAAG CTGGTGCAGG AGAAGAACGC CATGGTGCGC 
TACGAGTCAG AGCTGATGAT CTTTGCTCGG GAGCTGGAGC TGGAGGACCG GCAGAGCCGG 
CTGCAGCAGG AGCTCCGCGA GCGGATGGCG GTGGAAGACC ACCTGAAGAC GGAGGAGGAG 
CTGGCGGAGG AGAAGAGGAT CCTGAACGAG ATGCTGGAGG TGGTGGAGCA GAGAGACGCC 
CTGGTGGCCC TGCTGGAGGA GCAGCGGTTG CGGGAGAAGG AGGAGGACAA GGACCTGGAG 
GCCGCCATGC TGTCCAAGGG CTTCAGCCTG CACTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463589.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319075.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

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CloneID	OBa52136
Clone ID Related Accession (Same CDS sequence)	XM_015463589.1 , XM_010805726.2
Accession Version	XM_015463589.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CAAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319075.1
CDS	245..6604
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463589.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463589.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CAAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805726.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804028.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

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CloneID	OBa52136
Clone ID Related Accession (Same CDS sequence)	XM_015463589.1 , XM_010805726.2
Accession Version	XM_010805726.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805726.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CAAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804028.1
CDS	245..6604
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805726.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805726.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GAAGTATATA AAGATGTACA CGGGCGGAGT GAGCTCATTG 
GCTGAGCAGA TAGCCAATCA GCTTCAAAGG AAAGAACAAC CCAAAACCCT TCTAGACAAG 
AAGGAACTGG GCTCCATAAA GAAGGAGTTC CCGCAGAACT TGGGAGGCAG CGACACCTGC 
TATTTCTGCC AGAAGCGGGT CTATGTGATG GAGCGGCTGA GCGCCGAGGG CAAGTTCTTC 
CACCGGAGCT GCTTCAAGTG CGAGCACTGC GCCACCACCC TGCGCCTGTC GGCCTATGCC 
TACGCCCTCG AGGACGGTAA ATTCTACTGC AAGCCACACT ACTGTTACCG GCTGTCTGGC 
CCTGCACAGA GGAAGAGACC GGCAGGGGTG CCTCTGTCTG GAAAGGAGGC CCGGGGACCC 
CTGCAGGACA GCCCCGCCGC AGACGCCAGT GGACGGCCCA GCACCAGTGC CAGCCCTGCC 
GAGAGAAGCC CAGGCCCAAG CGTGAATGGC CTGGAGGAGC CGAGCGTCGC CAAGAGACTG 
CGGGGCACCC CGGAGAGGAT CGAGCTGGAG AACTACCGGC TGTCGGTGCG GCAGGCGGAG 
GGGCTGGAGG AGGTGCCCGA GGAGACGCAG GCCGAGCACA ACCTGAGCAG CGTGCTGGAC 
ACGGGCACAG AGGAGGACGC GGCCAGCAGC AGTTCGGAGT CGGAGATGGA GGAAGAGGAG 
CCTCCGCTGC CCACCTCGGA CCTGGGCGGC GTACCCTGGA AGGAGGCTGT GAGGATCCAT 
GCGCTCCTGA GAGGGAAGAG TGAAGAGGAG CTGGAGGCCT CCAGGAGCTT CGGGGCCGGG 
GAGGAGGAGG AGGAAGACGA GGAGGACGAG GAGGAGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGACGAGGA TGAGTCTAGT GAAGTCGGGA GCCCAAGGAG GCTGCAGCAG 
CTGCTGAACC CGGCCGACCC CCTGGAGATC CAGGCCGACG TGCACTGGAC GCATATCCGT 
GAGAGCCAGG AGGAGCGAGC GGCACTGGCG CCCAAGTCCC CCCTTCCCGG AGTGCCCGGC 
CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA 
TTCGATGAGG ATGACCTGGA GCGAGACGTG GACTCGGAAC CAGCAGAGAT CAAAGGGGAG 
GCAGCGGAGA ATGGGGACGC AGGGGACACT GGTGCCGAGC TGGACGACGA TCAGCACTGG 
TCGGATGACG TCCCGTCCGA GGCCAACACG GAGCTGCACC TGCCGGCAGT GGGAGCAGAG 
CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG GAGCCACCGC CGGCCTCAGT AGGGCACCCA 
GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC ACCCGGTCCC CTGAGGAGCC CACGGGCCTG 
TCCTCCCCAG CCCGCAGCCC CGGGGCACAG AGCGCCCATC CCCCACTTGC CGCTGTGGCC 
ACCGGGGTAA GGTCACCCGC CGAGAGCCCT CTGCCGGAGC CCTCGACCCC GCCAGCGGAG 
CCCGAAGCTC ACCCTCCCAT CCGATCGCAG CCTGAAGCCA GGACGCCACC CTCGCCTGCC 
AGCCCCCAGC GCCCATCCCC GCCCACCCAA CTGCCCATCT GCTCCCAGCC TCAGCCGTCC 
CCTGAGGCCA CCGTACCGTC CCCCACCCTG TCCCCCATCC GCTCCCAGCC CGTGCCGGCC 
AGAACCTCCA CTCCTCTGGC CCCCCTCCCC GTGAAGAACC AAGGGGTCAC GAAGGACACA 
CTGGGCAGCT CCCTCCCTGG GGACGAGGCC CTGAAACGCA GCGACCTGGT GGCAGAGTTC 
TGGATGAAGA GTGCCGAGAT CCGCCGCAGC CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG 
GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC 
CAGAGCGAGG GGCTCCGGCT CCTAAAACCC CCACCTGTCC CCAGGAAGCT GGGGCTGCCA 
GCAGCCGAGG GGGCCCAGCC CTGCCCACCC ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA 
AAGGGCCCCC GGGAGGAGCA CAGAGACCTG TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC 
AGCTCCTCCC GCACCAGGAC ACCGGGCAGC CAGAGCTTCA ACACCTCTGA CTCCACCATG 
CTCACGCCTC CTTCCAGCCC GCCGCCCCCG CCGCCTGACG AGGAGCCCGC TACCCTTCAC 
AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG GCCTCAGCAC CCCCACCCCC GGCTGTATGT 
GTGAGGCCCC CGCGGGAGCC CACCCAGCCC CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG 
GAGAGCGTGG ATGAGATTCC CTTTGCGGAT GATGTGGAGG ACACGTATGA TGACAACACA 
TGTGACGACC GCACGGAGGA CTCGAGTCTG CAGGAGACCT TCTTCACACC CCCCTCCCAC 
TGGCCCCACC CCAAGCAGCC CTTGGCTCCG GAGAACGGCC GGGGGCCAGA GAGTGCGGTC 
CCACTGCAGA AGCGGGGGCT GCCGCTGGTC TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG 
CGCATGCGGG CCCGGGAGAA GTCGGTGCGG AGCCAGGCAC TGAGGGACGC CATGGCCCGG 
CAGCTGAGCA GGATGAAGGA GATGGACATA GCAGCTGCAG CCCCCAGGAC CCCCAGGACC 
CCTGCACCCC GCAGAGCCAC TGCCGTGCCC CCCAAGGGCC CCGAGGAACC TGCCCCGAGG 
CACGAAGCCA CAAGTGAGGA GCTGCTGTCC CCTCCCTCGG ACTCAGGGGG CCCGGACGGC 
TCGGTCACCT CGTCAGAGGG CTCCAGTGGG AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT 
CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC 
AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC 
TCGGGGTACA GGAAGGACAA GAAGAAGAGT GATGGCCGGT CTTGCCCCAG CACCCCGTCC 
AGCGGGACCA CAGTGGACGC CGGCAAGCCC AGGGCATCTC CCGTCTCAAG AGCAGAGCTG 
CGGACGCGGC GCCAGCTGAG CTGCTCGGAG GACTCGGACC TCTCCAGTGA CGACGTCCTC 
GAGCGGACCT CCCAGAAGTC CAGAAAAGAG CCGAGAACTT ACACGGAGGA GGAGCTGAAC 
GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG 
AAACGGCTGC ACCGCGCGCA GATCATCCAG CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG 
CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG 
CCCAGTGGCG GGACTCCACG GCGTAGACCT CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA 
GGCATGGGCA AGAAGGATGA CCCCAAGCTG ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG 
AAGAACGCCA TGGTGCGCTA CGAGTCAGAG CTGATGATCT TTGCTCGGGA GCTGGAGCTG 
GAGGACCGGC AGAGCCGGCT GCAGCAGGAG CTCCGCGAGC GGATGGCGGT GGAAGACCAC 
CTGAAGACGG AGGAGGAGCT GGCGGAGGAG AAGAGGATCC TGAACGAGAT GCTGGAGGTG 
GTGGAGCAGA GAGACGCCCT GGTGGCCCTG CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG 
GAGGACAAGG ACCTGGAGGC CGCCATGCTG TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805727.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804029.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

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CloneID	OBa52137
Clone ID Related Accession (Same CDS sequence)	XM_010805727.2 , XM_015463590.1
Accession Version	XM_010805727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805727.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CAAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804029.1
CDS	245..6574
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805727.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805727.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CAAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463590.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319076.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

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CloneID	OBa52137
Clone ID Related Accession (Same CDS sequence)	XM_010805727.2 , XM_015463590.1
Accession Version	XM_015463590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CAAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319076.1
CDS	245..6574
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463590.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463590.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTGCCCGGC CTCCCCTCCG TCCGCCGCGC AGCAGTTCAG 
GCCTGGCTGG AGTCGGTCTC CGGAGTCCCA TTCGATGAGG ATGACCTGGA GCGAGACGTG 
GACTCGGAAC CAGCAGAGAT CAAAGGGGAG GCAGCGGAGA ATGGGGACGC AGGGGACACT 
GGTGCCGAGC TGGACGACGA TCAGCACTGG TCGGATGACG TCCCGTCCGA GGCCAACACG 
GAGCTGCACC TGCCGGCAGT GGGAGCAGAG CTGGAGCTGA GGGTGTCGGA TGGCGAGGAG 
GAGCCACCGC CGGCCTCAGT AGGGCACCCA GAGAGAGGTC CCTCCCGAGT CTCCAGCCCC 
ACCCGGTCCC CTGAGGAGCC CACGGGCCTG TCCTCCCCAG CCCGCAGCCC CGGGGCACAG 
AGCGCCCATC CCCCACTTGC CGCTGTGGCC ACCGGGGTAA GGTCACCCGC CGAGAGCCCT 
CTGCCGGAGC CCTCGACCCC GCCAGCGGAG CCCGAAGCTC ACCCTCCCAT CCGATCGCAG 
CCTGAAGCCA GGACGCCACC CTCGCCTGCC AGCCCCCAGC GCCCATCCCC GCCCACCCAA 
CTGCCCATCT GCTCCCAGCC TCAGCCGTCC CCTGAGGCCA CCGTACCGTC CCCCACCCTG 
TCCCCCATCC GCTCCCAGCC CGTGCCGGCC AGAACCTCCA CTCCTCTGGC CCCCCTCCCC 
GTGAAGAACC AAGGGGTCAC GAAGGACACA CTGGGCAGCT CCCTCCCTGG GGACGAGGCC 
CTGAAACGCA GCGACCTGGT GGCAGAGTTC TGGATGAAGA GTGCCGAGAT CCGCCGCAGC 
CTGGGGCTCA CGCCTGTGCA CCGGAGCCCG GGGTCTGAGC TCGCCTTCCA GTCCCCGCCC 
CTGAAGGCCT GTCCAGCAGA GAAGGCCCCC CAGAGCGAGG GGCTCCGGCT CCTAAAACCC 
CCACCTGTCC CCAGGAAGCT GGGGCTGCCA GCAGCCGAGG GGGCCCAGCC CTGCCCACCC 
ACCCCTGTGT CCCCGCCCGA CAGGGAGCCA AAGGGCCCCC GGGAGGAGCA CAGAGACCTG 
TCCAGCAGCT CGGGGCTGGG CCTGCAGGGC AGCTCCTCCC GCACCAGGAC ACCGGGCAGC 
CAGAGCTTCA ACACCTCTGA CTCCACCATG CTCACGCCTC CTTCCAGCCC GCCGCCCCCG 
CCGCCTGACG AGGAGCCCGC TACCCTTCAC AGGAAGCCAG CCCTGGCAGG GCAGCTTGTG 
GCCTCAGCAC CCCCACCCCC GGCTGTATGT GTGAGGCCCC CGCGGGAGCC CACCCAGCCC 
CCCCAGGAGG AGGCGCGGAA GTCTTTCGTG GAGAGCGTGG ATGAGATTCC CTTTGCGGAT 
GATGTGGAGG ACACGTATGA TGACAACACA TGTGACGACC GCACGGAGGA CTCGAGTCTG 
CAGGAGACCT TCTTCACACC CCCCTCCCAC TGGCCCCACC CCAAGCAGCC CTTGGCTCCG 
GAGAACGGCC GGGGGCCAGA GAGTGCGGTC CCACTGCAGA AGCGGGGGCT GCCGCTGGTC 
TCGGCCGAGG CCAAGGAGCT GGCGGCCGAG CGCATGCGGG CCCGGGAGAA GTCGGTGCGG 
AGCCAGGCAC TGAGGGACGC CATGGCCCGG CAGCTGAGCA GGATGAAGGA GATGGACATA 
GCAGCTGCAG CCCCCAGGAC CCCCAGGACC CCTGCACCCC GCAGAGCCAC TGCCGTGCCC 
CCCAAGGGCC CCGAGGAACC TGCCCCGAGG CACGAAGCCA CAAGTGAGGA GCTGCTGTCC 
CCTCCCTCGG ACTCAGGGGG CCCGGACGGC TCGGTCACCT CGTCAGAGGG CTCCAGTGGG 
AAGAGCAAGA AGAGGTCGTC CCTCTTCTCT CCTCGGAGAA GCAAGAAAGA GAAGAAGCCC 
AAAGGCGAGG GCCGGCCCCT GGAGAGGCCC AGCCCCGGCA CTCTGGAGGA AGCGGCCGCC 
AAGCCCAGGT CTCTGTGGAA GTCTGTCTTC TCGGGGTACA GGAAGGACAA GAAGAAGAGT 
GATGGCCGGT CTTGCCCCAG CACCCCGTCC AGCGGGACCA CAGTGGACGC CGGCAAGCCC 
AGGGCATCTC CCGTCTCAAG AGCAGAGCTG CGGACGCGGC GCCAGCTGAG CTGCTCGGAG 
GACTCGGACC TCTCCAGTGA CGACGTCCTC GAGCGGACCT CCCAGAAGTC CAGAAAAGAG 
CCGAGAACTT ACACGGAGGA GGAGCTGAAC GCCAAGCTGA CCCGGCGTGT GCAGAAGGCA 
GCTCGGAGGC AGGCCAAGCA GGAGGAGCTG AAACGGCTGC ACCGCGCGCA GATCATCCAG 
CGGCAGCTGG AGCAGGTGGA GGAGAAGCAG CGGCAGCTGG AGGAGAGGGG GGTCGCCGTG 
GAGAAGGCGC TGCGCGGGGA AGCAGTTGAG CCCAGTGGCG GGACTCCACG GCGTAGACCT 
CTCTCCTTCT GCCCTTGCTG TGTCCAGGAA GGCATGGGCA AGAAGGATGA CCCCAAGCTG 
ATGCAGGAGT GGTTCAAGCT GGTGCAGGAG AAGAACGCCA TGGTGCGCTA CGAGTCAGAG 
CTGATGATCT TTGCTCGGGA GCTGGAGCTG GAGGACCGGC AGAGCCGGCT GCAGCAGGAG 
CTCCGCGAGC GGATGGCGGT GGAAGACCAC CTGAAGACGG AGGAGGAGCT GGCGGAGGAG 
AAGAGGATCC TGAACGAGAT GCTGGAGGTG GTGGAGCAGA GAGACGCCCT GGTGGCCCTG 
CTGGAGGAGC AGCGGTTGCG GGAGAAGGAG GAGGACAAGG ACCTGGAGGC CGCCATGCTG 
TCCAAGGGCT TCAGCCTGCA CTGGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463591.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319077.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

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CloneID	OBa52139
Clone ID Related Accession (Same CDS sequence)	XM_015463591.1 , XM_010805729.2
Accession Version	XM_015463591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCAA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319077.1
CDS	245..6511
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463591.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463591.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCAA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805729.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804031.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

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CloneID	OBa52139
Clone ID Related Accession (Same CDS sequence)	XM_015463591.1 , XM_010805729.2
Accession Version	XM_010805729.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805729.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCAA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804031.1
CDS	245..6511
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQRRDKERSRTCPKKVITLSPPPTPPPCRAHGGQQTHRDFDADSRGKQSPQHERPEPEPSRRFLVDQWELSLSLRSASRPASPSSDSLRQKYIKMYTGGVSSLAEQIANQLQRKEQPKTLLDKKELGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805729.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805729.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGCGACG GGACAAGGAG 
CGTTCTCGGA CCTGCCCCAA AAAAGTGATC ACCCTCTCTC CTCCCCCTAC TCCGCCTCCC 
TGTCGGGCAC ATGGCGGCCA GCAGACACAC AGGGATTTTG ATGCCGACAG CCGTGGCAAG 
CAGAGCCCCC AGCACGAAAG GCCAGAGCCT GAGCCTTCTC GTCGATTTTT GGTCGATCAG 
TGGGAGCTTT CTCTAAGCCT CCGCTCTGCC AGCCGCCCCG CTTCCCCCTC TTCCGACTCC 
CTCCGACAGA AGTATATAAA GATGTACACG GGCGGAGTGA GCTCATTGGC TGAGCAGATA 
GCCAATCAGC TTCAAAGGAA AGAACAACCC AAAACCCTTC TAGACAAGAA GGAACTGGGC 
TCCATAAAGA AGGAGTTCCC GCAGAACTTG GGAGGCAGCG ACACCTGCTA TTTCTGCCAG 
AAGCGGGTCT ATGTGATGGA GCGGCTGAGC GCCGAGGGCA AGTTCTTCCA CCGGAGCTGC 
TTCAAGTGCG AGCACTGCGC CACCACCCTG CGCCTGTCGG CCTATGCCTA CGCCCTCGAG 
GACGGTAAAT TCTACTGCAA GCCACACTAC TGTTACCGGC TGTCTGGCCC TGCACAGAGG 
AAGAGACCGG CAGGGGTGCC TCTGTCTGGA AAGGAGGCCC GGGGACCCCT GCAGGACAGC 
CCCGCCGCAG ACGCCAGTGG ACGGCCCAGC ACCAGTGCCA GCCCTGCCGA GAGAAGCCCA 
GGCCCAAGCG TGAATGGCCT GGAGGAGCCG AGCGTCGCCA AGAGACTGCG GGGCACCCCG 
GAGAGGATCG AGCTGGAGAA CTACCGGCTG TCGGTGCGGC AGGCGGAGGG GCTGGAGGAG 
GTGCCCGAGG AGACGCAGGC CGAGCACAAC CTGAGCAGCG TGCTGGACAC GGGCACAGAG 
GAGGACGCGG CCAGCAGCAG TTCGGAGTCG GAGATGGAGG AAGAGGAGCC TCCGCTGCCC 
ACCTCGGACC TGGGCGGCGT ACCCTGGAAG GAGGCTGTGA GGATCCATGC GCTCCTGAGA 
GGGAAGAGTG AAGAGGAGCT GGAGGCCTCC AGGAGCTTCG GGGCCGGGGA GGAGGAGGAG 
GAAGACGAGG AGGACGAGGA GGAGGAGGAG GAGGAGGAAG ACGAGGAGGA CGAGGAGGAG 
GACGAGGATG AGTCTAGTGA AGTCCCATTC GATGAGGATG ACCTGGAGCG AGACGTGGAC 
TCGGAACCAG CAGAGATCAA AGGGGAGGCA GCGGAGAATG GGGACGCAGG GGACACTGGT 
GCCGAGCTGG ACGACGATCA GCACTGGTCG GATGACGTCC CGTCCGAGGC CAACACGGAG 
CTGCACCTGC CGGCAGTGGG AGCAGAGCTG GAGCTGAGGG TGTCGGATGG CGAGGAGGAG 
CCACCGCCGG CCTCAGTAGG GCACCCAGAG AGAGGTCCCT CCCGAGTCTC CAGCCCCACC 
CGGTCCCCTG AGGAGCCCAC GGGCCTGTCC TCCCCAGCCC GCAGCCCCGG GGCACAGAGC 
GCCCATCCCC CACTTGCCGC TGTGGCCACC GGGGTAAGGT CACCCGCCGA GAGCCCTCTG 
CCGGAGCCCT CGACCCCGCC AGCGGAGCCC GAAGCTCACC CTCCCATCCG ATCGCAGCCT 
GAAGCCAGGA CGCCACCCTC GCCTGCCAGC CCCCAGCGCC CATCCCCGCC CACCCAACTG 
CCCATCTGCT CCCAGCCTCA GCCGTCCCCT GAGGCCACCG TACCGTCCCC CACCCTGTCC 
CCCATCCGCT CCCAGCCCGT GCCGGCCAGA ACCTCCACTC CTCTGGCCCC CCTCCCCGTG 
AAGAACCAAG GGGTCACGAA GGACACACTG GGCAGCTCCC TCCCTGGGGA CGAGGCCCTG 
AAACGCAGCG ACCTGGTGGC AGAGTTCTGG ATGAAGAGTG CCGAGATCCG CCGCAGCCTG 
GGGCTCACGC CTGTGCACCG GAGCCCGGGG TCTGAGCTCG CCTTCCAGTC CCCGCCCCTG 
AAGGCCTGTC CAGCAGAGAA GGCCCCCCAG AGCGAGGGGC TCCGGCTCCT AAAACCCCCA 
CCTGTCCCCA GGAAGCTGGG GCTGCCAGCA GCCGAGGGGG CCCAGCCCTG CCCACCCACC 
CCTGTGTCCC CGCCCGACAG GGAGCCAAAG GGCCCCCGGG AGGAGCACAG AGACCTGTCC 
AGCAGCTCGG GGCTGGGCCT GCAGGGCAGC TCCTCCCGCA CCAGGACACC GGGCAGCCAG 
AGCTTCAACA CCTCTGACTC CACCATGCTC ACGCCTCCTT CCAGCCCGCC GCCCCCGCCG 
CCTGACGAGG AGCCCGCTAC CCTTCACAGG AAGCCAGCCC TGGCAGGGCA GCTTGTGGCC 
TCAGCACCCC CACCCCCGGC TGTATGTGTG AGGCCCCCGC GGGAGCCCAC CCAGCCCCCC 
CAGGAGGAGG CGCGGAAGTC TTTCGTGGAG AGCGTGGATG AGATTCCCTT TGCGGATGAT 
GTGGAGGACA CGTATGATGA CAACACATGT GACGACCGCA CGGAGGACTC GAGTCTGCAG 
GAGACCTTCT TCACACCCCC CTCCCACTGG CCCCACCCCA AGCAGCCCTT GGCTCCGGAG 
AACGGCCGGG GGCCAGAGAG TGCGGTCCCA CTGCAGAAGC GGGGGCTGCC GCTGGTCTCG 
GCCGAGGCCA AGGAGCTGGC GGCCGAGCGC ATGCGGGCCC GGGAGAAGTC GGTGCGGAGC 
CAGGCACTGA GGGACGCCAT GGCCCGGCAG CTGAGCAGGA TGAAGGAGAT GGACATAGCA 
GCTGCAGCCC CCAGGACCCC CAGGACCCCT GCACCCCGCA GAGCCACTGC CGTGCCCCCC 
AAGGGCCCCG AGGAACCTGC CCCGAGGCAC GAAGCCACAA GTGAGGAGCT GCTGTCCCCT 
CCCTCGGACT CAGGGGGCCC GGACGGCTCG GTCACCTCGT CAGAGGGCTC CAGTGGGAAG 
AGCAAGAAGA GGTCGTCCCT CTTCTCTCCT CGGAGAAGCA AGAAAGAGAA GAAGCCCAAA 
GGCGAGGGCC GGCCCCTGGA GAGGCCCAGC CCCGGCACTC TGGAGGAAGC GGCCGCCAAG 
CCCAGGTCTC TGTGGAAGTC TGTCTTCTCG GGGTACAGGA AGGACAAGAA GAAGAGTGAT 
GGCCGGTCTT GCCCCAGCAC CCCGTCCAGC GGGACCACAG TGGACGCCGG CAAGCCCAGG 
GCATCTCCCG TCTCAAGAGC AGAGCTGCGG ACGCGGCGCC AGCTGAGCTG CTCGGAGGAC 
TCGGACCTCT CCAGTGACGA CGTCCTCGAG CGGACCTCCC AGAAGTCCAG AAAAGAGCCG 
AGAACTTACA CGGAGGAGGA GCTGAACGCC AAGCTGACCC GGCGTGTGCA GAAGGCAGCT 
CGGAGGCAGG CCAAGCAGGA GGAGCTGAAA CGGCTGCACC GCGCGCAGAT CATCCAGCGG 
CAGCTGGAGC AGGTGGAGGA GAAGCAGCGG CAGCTGGAGG AGAGGGGGGT CGCCGTGGAG 
AAGGCGCTGC GCGGGGAAGC AGTTGAGCCC AGTGGCGGGA CTCCACGGCG TAGACCTCTC 
TCCTTCTGCC CTTGCTGTGT CCAGGAAGGC ATGGGCAAGA AGGATGACCC CAAGCTGATG 
CAGGAGTGGT TCAAGCTGGT GCAGGAGAAG AACGCCATGG TGCGCTACGA GTCAGAGCTG 
ATGATCTTTG CTCGGGAGCT GGAGCTGGAG GACCGGCAGA GCCGGCTGCA GCAGGAGCTC 
CGCGAGCGGA TGGCGGTGGA AGACCACCTG AAGACGGAGG AGGAGCTGGC GGAGGAGAAG 
AGGATCCTGA ACGAGATGCT GGAGGTGGTG GAGCAGAGAG ACGCCCTGGT GGCCCTGCTG 
GAGGAGCAGC GGTTGCGGGA GAAGGAGGAG GACAAGGACC TGGAGGCCGC CATGCTGTCC 
AAGGGCTTCA GCCTGCACTG GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_015463592.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319078.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

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CloneID	OBa52140
Clone ID Related Accession (Same CDS sequence)	XM_015463592.1 , XM_010805730.2
Accession Version	XM_015463592.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644860.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCAAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319078.1
CDS	244..6339
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_015463592.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463592.1

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCAAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL3 ( XM_010805730.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804032.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

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CloneID	OBa52140
Clone ID Related Accession (Same CDS sequence)	XM_015463592.1 , XM_010805730.2
Accession Version	XM_010805730.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103943.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805730.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCAAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804032.1
CDS	244..6339
Translation	MEESKNEATNRAHVLFDRFVQATTCKGTLKAFQELCDHLELKPKDHRSFYHKLKSKLNYWKAKALWAKLDKRGSHKDYKKGKVCTNTKCLIIGAGPCGLRTAIDLSLLGAKVVVIEKRDAFSRNNVLHLWPFTIHDLRGLGAKKFYGKFCAGAIDHISIRQLQLILLKVALILGIEIHVNVEFRGLVEPPEDQENERIGWRALVHPKTHPVSEYEFEVIIGGDGRRNTLEGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQKFFQELREATGIDLENIVYYKDDTHYFVMTAKKQSLLDKGVILHDYADTELLLSRENVDQEALLSYAREAADFSTQQQLPSLDFAINHYGQPDVAMFDFTCMYASENAALVREHNGHQLLVALVGDSLLEPFWPMGTGIARGFLAAMDSAWMVRSWSLGTSPLEVLAERESIYRLLPQTTPENVSKNFSQYSIDPVTRYPNVNVNFLRPSQVRHLYDTGDTKDVHLEMENLVNSRTTPKLARNESVARSSKLLGWCQRQTDGYAGVNVTDLTMSWKSGLALCAIIHRYRPDLIDFDSLDEQNVEKNNQLAFDIAEKELGISPIMTGREMASVGEPDKLSMVMYLTQFYEMFKDSLPSRDASDLNAEERAVLIASTKSPISFLSKLGQTISRKRSPKDKKEKDLDGAGKRRKTSQSEEEDTPRGHRGARPTLVSTLTDRRMDVALGNQNKVKYMATQLLAKFEENAPPQSVGVRRQPTQERGVSQPSSCLPEQGRSAPTPQWKQGSIKKEFPQNLGGSDTCYFCQKRVYVMERLSAEGKFFHRSCFKCEHCATTLRLSAYAYALEDGKFYCKPHYCYRLSGPAQRKRPAGVPLSGKEARGPLQDSPAADASGRPSTSASPAERSPGPSVNGLEEPSVAKRLRGTPERIELENYRLSVRQAEGLEEVPEETQAEHNLSSVLDTGTEEDAASSSSESEMEEEEPPLPTSDLGGVPWKEAVRIHALLRGKSEEELEASRSFGAGEEEEEDEEDEEEEEEEEDEEDEEEDEDESSEVGSPRRLQQLLNPADPLEIQADVHWTHIRESQEERAALAPKSPLPGVPGLPSVRRAAVQAWLESVSGVPFDEDDLERDVDSEPAEIKGEAAENGDAGDTGAELDDDQHWSDDVPSEANTELHLPAVGAELELRVSDGEEEPPPASVGHPERGPSRVSSPTRSPEEPTGLSSPARSPGAQSAHPPLAAVATGVRSPAESPLPEPSTPPAEPEAHPPIRSQPEARTPPSPASPQRPSPPTQLPICSQPQPSPEATVPSPTLSPIRSQPVPARTSTPLAPLPVKNQGVTKDTLGSSLPGDEALKRSDLVAEFWMKSAEIRRSLGLTPVHRSPGSELAFQSPPLKACPAEKAPQSEGLRLLKPPPVPRKLGLPAAEGAQPCPPTPVSPPDREPKGPREEHRDLSSSSGLGLQGSSSRTRTPGSQSFNTSDSTMLTPPSSPPPPPPDEEPATLHRKPALAGQLVASAPPPPAVCVRPPREPTQPPQEEARKSFVESVDEIPFADDVEDTYDDNTCDDRTEDSSLQETFFTPPSHWPHPKQPLAPENGRGPESAVPLQKRGLPLVSAEAKELAAERMRAREKSVRSQALRDAMARQLSRMKEMDIAAAAPRTPRTPAPRRATAVPPKGPEEPAPRHEATSEELLSPPSDSGGPDGSVTSSEGSSGKSKKRSSLFSPRRSKKEKKPKGEGRPLERPSPGTLEEAAAKPRSLWKSVFSGYRKDKKKSDGRSCPSTPSSGTTVDAGKPRASPVSRAELRTRRQLSCSEDSDLSSDDVLERTSQKSRKEPRTYTEEELNAKLTRRVQKAARRQAKQEELKRLHRAQIIQRQLEQVEEKQRQLEERGVAVEKALRGEAVEPSGGTPRRRPLSFCPCCVQEGMGKKDDPKLMQEWFKLVQEKNAMVRYESELMIFARELELEDRQSRLQQELRERMAVEDHLKTEEELAEEKRILNEMLEVVEQRDALVALLEEQRLREKEEDKDLEAAMLSKGFSLHWS

Target ORF information:

RefSeq Version	XM_010805730.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 3 (MICAL3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805730.2

ORF Insert Sequence:

ATGGAGGAGA GTAAGAACGA GGCCACGAAT CGGGCCCACG TCCTCTTTGA CCGCTTCGTG 
CAGGCCACCA CCTGCAAGGG GACCCTCAAG GCCTTCCAAG AGCTCTGTGA CCACCTGGAA 
CTGAAGCCTA AGGACCATCG CTCCTTTTAT CACAAGCTCA AGTCCAAGCT TAACTACTGG 
AAAGCCAAGG CCCTCTGGGC AAAGCTGGAC AAGCGGGGCA GCCACAAAGA CTACAAGAAG 
GGAAAGGTGT GCACGAACAC CAAGTGTCTC ATCATTGGAG CCGGCCCCTG CGGTCTCCGC 
ACAGCCATCG ATTTGTCCTT GCTGGGAGCT AAGGTGGTCG TCATTGAGAA GCGAGACGCC 
TTCTCCCGCA ACAACGTCTT GCACCTCTGG CCGTTCACCA TACATGACCT GCGAGGTCTG 
GGTGCCAAGA AGTTCTACGG CAAGTTCTGT GCTGGGGCCA TCGACCACAT CAGTATCCGT 
CAGCTTCAGC TAATACTTTT GAAAGTAGCC TTGATCTTAG GCATTGAGAT CCACGTCAAT 
GTGGAATTCC GAGGACTTGT GGAGCCTCCC GAGGATCAAG AAAATGAACG AATAGGTTGG 
CGGGCCCTGG TGCACCCCAA AACGCACCCT GTGTCAGAAT ATGAGTTTGA AGTGATCATC 
GGCGGGGATG GCCGCAGGAA TACCTTGGAA GGATTTCGTC GGAAAGAATT CCGTGGCAAA 
TTGGCCATTG CCATTACAGC AAATTTTATT AACCGAAATA CAACAGCAGA AGCCAAAGTG 
GAAGAAATCA GTGGTGTGGC TTTTATATTC AACCAAAAAT TTTTCCAGGA ACTGAGGGAA 
GCCACAGGGA TCGACCTGGA GAACATTGTC TACTACAAAG ATGACACACA CTACTTCGTT 
ATGACTGCCA AGAAGCAGAG CTTACTGGAC AAAGGGGTGA TACTGCATGA CTACGCTGAC 
ACGGAGCTCT TGCTCTCCCG GGAGAATGTG GACCAGGAGG CCCTGCTGAG CTACGCCCGG 
GAGGCAGCCG ACTTCTCCAC CCAGCAGCAG CTGCCGTCGC TGGACTTCGC CATCAACCAC 
TACGGGCAGC CTGACGTGGC CATGTTCGAC TTCACGTGCA TGTATGCCTC CGAGAATGCG 
GCCCTGGTGC GGGAGCACAA CGGGCACCAG CTGCTGGTGG CTCTGGTGGG GGACAGCCTC 
CTGGAGCCTT TTTGGCCAAT GGGAACAGGA ATAGCCCGGG GCTTTCTGGC TGCTATGGAC 
TCAGCCTGGA TGGTGCGTAG CTGGTCTCTG GGGACAAGCC CCTTGGAAGT CCTGGCAGAG 
AGGGAAAGCA TTTATAGGTT GCTGCCTCAG ACCACCCCTG AGAACGTGAG CAAGAACTTC 
AGCCAGTACA GCATCGACCC GGTCACCAGA TACCCCAACG TGAATGTCAA CTTTCTCCGG 
CCGAGTCAGG TGCGCCATCT GTATGATACT GGAGACACAA AAGACGTTCA TTTGGAGATG 
GAGAACCTTG TGAATTCCCG AACCACCCCA AAGCTGGCCC GCAATGAGTC TGTGGCTCGT 
TCCAGCAAGC TGCTGGGTTG GTGCCAGCGG CAGACCGATG GCTATGCTGG GGTGAATGTG 
ACGGACCTCA CCATGTCGTG GAAGAGTGGC CTGGCCCTGT GTGCCATCAT ACACAGATAC 
CGCCCTGACC TGATAGATTT TGACTCTTTG GATGAGCAGA ATGTGGAGAA GAATAACCAG 
CTGGCCTTCG ACATTGCTGA GAAAGAGCTG GGCATTTCTC CCATCATGAC AGGCAGAGAG 
ATGGCCTCAG TAGGGGAGCC GGACAAGCTA TCCATGGTTA TGTACCTGAC CCAGTTCTAC 
GAGATGTTCA AGGACTCCCT CCCCTCCCGA GACGCCTCAG ACCTGAATGC GGAGGAGAGG 
GCTGTCCTAA TAGCCAGCAC CAAGTCCCCC ATCTCCTTCC TGAGCAAACT CGGGCAGACT 
ATTTCTCGGA AGCGCTCCCC CAAGGACAAG AAGGAAAAAG ACTTGGATGG TGCTGGGAAG 
AGGAGAAAGA CCAGCCAGTC AGAGGAGGAG GACACGCCCC GGGGCCACAG AGGAGCAAGG 
CCCACCCTGG TGAGCACGCT GACCGACCGG AGGATGGACG TGGCCCTCGG CAACCAGAAC 
AAAGTGAAGT ACATGGCAAC CCAGCTGCTG GCCAAGTTTG AGGAGAACGC GCCTCCACAG 
TCTGTGGGTG TGAGGAGACA GCCGACACAG GAGCGTGGGG TCAGCCAGCC GTCCAGCTGC 
CTGCCCGAGC AGGGTCGCTC TGCTCCCACC CCCCAGTGGA AACAGGGCTC CATAAAGAAG 
GAGTTCCCGC AGAACTTGGG AGGCAGCGAC ACCTGCTATT TCTGCCAGAA GCGGGTCTAT 
GTGATGGAGC GGCTGAGCGC CGAGGGCAAG TTCTTCCACC GGAGCTGCTT CAAGTGCGAG 
CACTGCGCCA CCACCCTGCG CCTGTCGGCC TATGCCTACG CCCTCGAGGA CGGTAAATTC 
TACTGCAAGC CACACTACTG TTACCGGCTG TCTGGCCCTG CACAGAGGAA GAGACCGGCA 
GGGGTGCCTC TGTCTGGAAA GGAGGCCCGG GGACCCCTGC AGGACAGCCC CGCCGCAGAC 
GCCAGTGGAC GGCCCAGCAC CAGTGCCAGC CCTGCCGAGA GAAGCCCAGG CCCAAGCGTG 
AATGGCCTGG AGGAGCCGAG CGTCGCCAAG AGACTGCGGG GCACCCCGGA GAGGATCGAG 
CTGGAGAACT ACCGGCTGTC GGTGCGGCAG GCGGAGGGGC TGGAGGAGGT GCCCGAGGAG 
ACGCAGGCCG AGCACAACCT GAGCAGCGTG CTGGACACGG GCACAGAGGA GGACGCGGCC 
AGCAGCAGTT CGGAGTCGGA GATGGAGGAA GAGGAGCCTC CGCTGCCCAC CTCGGACCTG 
GGCGGCGTAC CCTGGAAGGA GGCTGTGAGG ATCCATGCGC TCCTGAGAGG GAAGAGTGAA 
GAGGAGCTGG AGGCCTCCAG GAGCTTCGGG GCCGGGGAGG AGGAGGAGGA AGACGAGGAG 
GACGAGGAGG AGGAGGAGGA GGAGGAAGAC GAGGAGGACG AGGAGGAGGA CGAGGATGAG 
TCTAGTGAAG TCGGGAGCCC AAGGAGGCTG CAGCAGCTGC TGAACCCGGC CGACCCCCTG 
GAGATCCAGG CCGACGTGCA CTGGACGCAT ATCCGTGAGA GCCAGGAGGA GCGAGCGGCA 
CTGGCGCCCA AGTCCCCCCT TCCCGGAGTG CCCGGCCTCC CCTCCGTCCG CCGCGCAGCA 
GTTCAGGCCT GGCTGGAGTC GGTCTCCGGA GTCCCATTCG ATGAGGATGA CCTGGAGCGA 
GACGTGGACT CGGAACCAGC AGAGATCAAA GGGGAGGCAG CGGAGAATGG GGACGCAGGG 
GACACTGGTG CCGAGCTGGA CGACGATCAG CACTGGTCGG ATGACGTCCC GTCCGAGGCC 
AACACGGAGC TGCACCTGCC GGCAGTGGGA GCAGAGCTGG AGCTGAGGGT GTCGGATGGC 
GAGGAGGAGC CACCGCCGGC CTCAGTAGGG CACCCAGAGA GAGGTCCCTC CCGAGTCTCC 
AGCCCCACCC GGTCCCCTGA GGAGCCCACG GGCCTGTCCT CCCCAGCCCG CAGCCCCGGG 
GCACAGAGCG CCCATCCCCC ACTTGCCGCT GTGGCCACCG GGGTAAGGTC ACCCGCCGAG 
AGCCCTCTGC CGGAGCCCTC GACCCCGCCA GCGGAGCCCG AAGCTCACCC TCCCATCCGA 
TCGCAGCCTG AAGCCAGGAC GCCACCCTCG CCTGCCAGCC CCCAGCGCCC ATCCCCGCCC 
ACCCAACTGC CCATCTGCTC CCAGCCTCAG CCGTCCCCTG AGGCCACCGT ACCGTCCCCC 
ACCCTGTCCC CCATCCGCTC CCAGCCCGTG CCGGCCAGAA CCTCCACTCC TCTGGCCCCC 
CTCCCCGTGA AGAACCAAGG GGTCACGAAG GACACACTGG GCAGCTCCCT CCCTGGGGAC 
GAGGCCCTGA AACGCAGCGA CCTGGTGGCA GAGTTCTGGA TGAAGAGTGC CGAGATCCGC 
CGCAGCCTGG GGCTCACGCC TGTGCACCGG AGCCCGGGGT CTGAGCTCGC CTTCCAGTCC 
CCGCCCCTGA AGGCCTGTCC AGCAGAGAAG GCCCCCCAGA GCGAGGGGCT CCGGCTCCTA 
AAACCCCCAC CTGTCCCCAG GAAGCTGGGG CTGCCAGCAG CCGAGGGGGC CCAGCCCTGC 
CCACCCACCC CTGTGTCCCC GCCCGACAGG GAGCCAAAGG GCCCCCGGGA GGAGCACAGA 
GACCTGTCCA GCAGCTCGGG GCTGGGCCTG CAGGGCAGCT CCTCCCGCAC CAGGACACCG 
GGCAGCCAGA GCTTCAACAC CTCTGACTCC ACCATGCTCA CGCCTCCTTC CAGCCCGCCG 
CCCCCGCCGC CTGACGAGGA GCCCGCTACC CTTCACAGGA AGCCAGCCCT GGCAGGGCAG 
CTTGTGGCCT CAGCACCCCC ACCCCCGGCT GTATGTGTGA GGCCCCCGCG GGAGCCCACC 
CAGCCCCCCC AGGAGGAGGC GCGGAAGTCT TTCGTGGAGA GCGTGGATGA GATTCCCTTT 
GCGGATGATG TGGAGGACAC GTATGATGAC AACACATGTG ACGACCGCAC GGAGGACTCG 
AGTCTGCAGG AGACCTTCTT CACACCCCCC TCCCACTGGC CCCACCCCAA GCAGCCCTTG 
GCTCCGGAGA ACGGCCGGGG GCCAGAGAGT GCGGTCCCAC TGCAGAAGCG GGGGCTGCCG 
CTGGTCTCGG CCGAGGCCAA GGAGCTGGCG GCCGAGCGCA TGCGGGCCCG GGAGAAGTCG 
GTGCGGAGCC AGGCACTGAG GGACGCCATG GCCCGGCAGC TGAGCAGGAT GAAGGAGATG 
GACATAGCAG CTGCAGCCCC CAGGACCCCC AGGACCCCTG CACCCCGCAG AGCCACTGCC 
GTGCCCCCCA AGGGCCCCGA GGAACCTGCC CCGAGGCACG AAGCCACAAG TGAGGAGCTG 
CTGTCCCCTC CCTCGGACTC AGGGGGCCCG GACGGCTCGG TCACCTCGTC AGAGGGCTCC 
AGTGGGAAGA GCAAGAAGAG GTCGTCCCTC TTCTCTCCTC GGAGAAGCAA GAAAGAGAAG 
AAGCCCAAAG GCGAGGGCCG GCCCCTGGAG AGGCCCAGCC CCGGCACTCT GGAGGAAGCG 
GCCGCCAAGC CCAGGTCTCT GTGGAAGTCT GTCTTCTCGG GGTACAGGAA GGACAAGAAG 
AAGAGTGATG GCCGGTCTTG CCCCAGCACC CCGTCCAGCG GGACCACAGT GGACGCCGGC 
AAGCCCAGGG CATCTCCCGT CTCAAGAGCA GAGCTGCGGA CGCGGCGCCA GCTGAGCTGC 
TCGGAGGACT CGGACCTCTC CAGTGACGAC GTCCTCGAGC GGACCTCCCA GAAGTCCAGA 
AAAGAGCCGA GAACTTACAC GGAGGAGGAG CTGAACGCCA AGCTGACCCG GCGTGTGCAG 
AAGGCAGCTC GGAGGCAGGC CAAGCAGGAG GAGCTGAAAC GGCTGCACCG CGCGCAGATC 
ATCCAGCGGC AGCTGGAGCA GGTGGAGGAG AAGCAGCGGC AGCTGGAGGA GAGGGGGGTC 
GCCGTGGAGA AGGCGCTGCG CGGGGAAGCA GTTGAGCCCA GTGGCGGGAC TCCACGGCGT 
AGACCTCTCT CCTTCTGCCC TTGCTGTGTC CAGGAAGGCA TGGGCAAGAA GGATGACCCC 
AAGCTGATGC AGGAGTGGTT CAAGCTGGTG CAGGAGAAGA ACGCCATGGT GCGCTACGAG 
TCAGAGCTGA TGATCTTTGC TCGGGAGCTG GAGCTGGAGG ACCGGCAGAG CCGGCTGCAG 
CAGGAGCTCC GCGAGCGGAT GGCGGTGGAA GACCACCTGA AGACGGAGGA GGAGCTGGCG 
GAGGAGAAGA GGATCCTGAA CGAGATGCTG GAGGTGGTGG AGCAGAGAGA CGCCCTGGTG 
GCCCTGCTGG AGGAGCAGCG GTTGCGGGAG AAGGAGGAGG ACAAGGACCT GGAGGCCGCC 
ATGCTGTCCA AGGGCTTCAG CCTGCACTGG TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MICAL3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: