CEP250 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005215005.3	XP_005215062.1	centrosome-associated protein CEP250 isoform X1
XM_015474286.1	XP_015329772.1	centrosome-associated protein CEP250 isoform X5
XM_015474287.2	XP_015329773.2	centrosome-associated protein CEP250 isoform X5
XM_015466170.1	XP_015321656.1	centrosome-associated protein CEP250 isoform X5
XM_015466171.1	XP_015321657.1	centrosome-associated protein CEP250 isoform X6
XM_005215004.3	XP_005215061.1	centrosome-associated protein CEP250 isoform X1
XM_015474287.1	XP_015329773.1	centrosome-associated protein CEP250 isoform X6
XM_005215004.4	XP_005215061.2	centrosome-associated protein CEP250 isoform X1
XM_015474285.2	XP_015329771.2	centrosome-associated protein CEP250 isoform X4
XM_002684296.5	XP_002684342.2	centrosome-associated protein CEP250 isoform X1
XM_005192478.3	XP_005192535.2	centrosome-associated protein CEP250 isoform X1
XM_015474285.1	XP_015329771.1	centrosome-associated protein CEP250 isoform X4
XM_015466168.1	XP_015321654.1	centrosome-associated protein CEP250 isoform X4
XM_015474284.2	XP_015329770.2	centrosome-associated protein CEP250 isoform X3
XM_010824119.2	XP_010822421.2	centrosome-associated protein CEP250 isoform X2
XM_010811675.2	XP_010809977.1	centrosome-associated protein CEP250 isoform X2
XM_005215005.4	XP_005215062.2	centrosome-associated protein CEP250 isoform X1
XM_010811675.3	XP_010809977.2	centrosome-associated protein CEP250 isoform X2
XM_025001207.1	XP_024856975.1	centrosome-associated protein CEP250 isoform X4
XM_015466167.1	XP_015321653.1	centrosome-associated protein CEP250 isoform X3
XM_015474284.1	XP_015329770.1	centrosome-associated protein CEP250 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222056	XM_015474287.2 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222052	XM_005215005.4 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa222052	XM_005215004.4 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa222053	XM_010811675.3 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa222054	XM_015474284.2 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OBa222055	XM_015474285.2 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa222055	XM_025001207.1 Latest version!	Bos taurus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa25630	XM_002684296.5 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa25630	XM_005215005.3 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa25630	XM_005192478.3 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa25630	XM_005215004.3 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa25632	XM_015474285.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa25632	XM_015466168.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa46857	XM_010811675.2 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa46857	XM_010824119.2 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa92412	XM_015466167.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa92412	XM_015474284.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OBa92413	XM_015466170.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa92413	XM_015474286.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OBa92414	XM_015466171.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa92414	XM_015474287.1 Latest version!	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

CEP250 ( XM_015474287.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329773.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa222056
Clone ID Related Accession (Same CDS sequence)	XM_015474287.2
Accession Version	XM_015474287.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAC GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCAAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTAA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCGG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329773.2
CDS	177..5579
Translation	MEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474287.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474287.2

ORF Insert Sequence:

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAC GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCAAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTAA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCGG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005215004.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215061.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OBa222052
Clone ID Related Accession (Same CDS sequence)	XM_005215004.4 , XM_005215005.4
Accession Version	XM_005215004.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215004.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ACGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCAAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT AAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC GGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215061.2
CDS	218..7522
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_005215004.4
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215004.4

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ACGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCAAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT AAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC GGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005215005.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215062.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OBa222052
Clone ID Related Accession (Same CDS sequence)	XM_005215004.4 , XM_005215005.4
Accession Version	XM_005215005.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215005.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ACGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCAAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT AAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC GGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215062.2
CDS	211..7515
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_005215005.4
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215005.4

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ACGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCAAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT AAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC GGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_010811675.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809977.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OBa222053
Clone ID Related Accession (Same CDS sequence)	XM_010811675.3
Accession Version	XM_010811675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAACGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCAAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTAAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCGGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809977.2
CDS	216..7442
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGCRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_010811675.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811675.3

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAACGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCAAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTAAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCGGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474284.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329770.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OBa222054
Clone ID Related Accession (Same CDS sequence)	XM_015474284.2
Accession Version	XM_015474284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAACGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCAAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTAAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCGGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329770.2
CDS	136..7332
Translation	MEEAEELPGGPAEASSSREEAAGQGVTVPELVPGAGEAAGSHWGTDPSKVGKRGKAKPGTAANPIGRGTTECRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474284.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474284.2

ORF Insert Sequence:

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAACGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCAAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTAAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCGGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474285.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329771.2 Bos taurus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OBa222055
Clone ID Related Accession (Same CDS sequence)	XM_015474285.2 , XM_025001207.1
Accession Version	XM_015474285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AACGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCAAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TAAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CGGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329771.2
CDS	118..7053
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474285.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474285.2

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AACGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCAAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TAAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CGGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_025001207.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024856975.1 Bos taurus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

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CloneID	OBa222055
Clone ID Related Accession (Same CDS sequence)	XM_015474285.2 , XM_025001207.1
Accession Version	XM_025001207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AACGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCAAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TAAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CGGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024856975.1
CDS	140..7075
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELREAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_025001207.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001207.1

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AACGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCAAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TAAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CGGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005215005.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215062.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

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CloneID	OBa25630
Clone ID Related Accession (Same CDS sequence)	XM_005215005.3 , XM_005215004.3 , XM_002684296.5 , XM_005192478.3
Accession Version	XM_005215005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215005.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215062.1
CDS	210..7514
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_005215005.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215005.3

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005215004.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215061.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

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CloneID	OBa25630
Clone ID Related Accession (Same CDS sequence)	XM_005215005.3 , XM_005215004.3 , XM_002684296.5 , XM_005192478.3
Accession Version	XM_005215004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215061.1
CDS	212..7516
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_005215004.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215004.3

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_002684296.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002684342.2 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

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CloneID	OBa25630
Clone ID Related Accession (Same CDS sequence)	XM_005215005.3 , XM_005215004.3 , XM_002684296.5 , XM_005192478.3
Accession Version	XM_002684296.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002684296.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002684342.2
CDS	212..7516
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_002684296.5
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002684296.5

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_005192478.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005192535.2 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

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CloneID	OBa25630
Clone ID Related Accession (Same CDS sequence)	XM_005215005.3 , XM_005215004.3 , XM_002684296.5 , XM_005192478.3
Accession Version	XM_005192478.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005192478.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005192535.2
CDS	210..7514
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGGPIPQRWESVEKPNLEQLLIRLEEEQQRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_005192478.3
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005192478.3

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGGACCGA TCCCTCAAAG GTGGGAAAGC GTGGAAAAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AAGAGGAACA ACAGAGGTGT GAGAATCTAG TGGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTCG ACTGGAGCCG GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ACGGTCGCCT TCTGGGTCTA 
TGGCGGGAGG TGGTGACTTT CCGACGCCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGACGG AGCTCAAGGC TGAGCACGCG AGGCTTTCGG GGTCCCTGTT GACCTCTTGC 
CTGCGCCTGA CTGTGGGAGT ACCACCATCT CGGGAGTCTG ATGGATCTGG GAGACTGAAT 
GGAAACGAGC CATCCCAGTT GATGCTGCTA CTAGCCAAGA CCCAGGAACT GGAGAGGGAA 
GCCCATGAAA GGAGCCAGGA GTTAATACAG CTGAAGAGTC AAGGGGATCT GGAGAAGGCA 
GAGCTTCAGG ATCGGGTGAC TGAGCTCTCT GCTCTGCTGA CCCAGTCTCA GAAGCAAAAT 
GAAGATTATG AAAAGATGGT GAAGGCTCTG AGAGCGACGA TGGAGATCCT GGAGGCAAAT 
CATGCAGAAT TAATGGAACA CGAGGCATCT CTTAGTAGGA ACGCCCAAGA GGAGAAGTTG 
TCTTTACAGC AAGTGATCCA GGATATAACC CAGGCCATGG TAGAAGAAGA GGACAGTATG 
GCCCAAGGTT CTAGTCACGA GAGCTCCTTG CAGTTGGACC CTGGTGGCTT CTCTTCCCGG 
TTTGATTCCC AGCAGCCAGA CAAGGCTGTC TCTCTGGTGC GTTCGGTACT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAGGCAGCAG CTGTCAGGCT GCCAGGAAGC TGTGAGCTCC 
TGGCAACAGC AGCATCACCA GTGGGAGGAA GAGGGTGAAG CCCTGAGACA GCGGCTGCAG 
CAGCTCACAG GGGAGCGGGA CACCCTGGCG GGGCAGACGG TGGACCTGCA GGGAGAGGTG 
GACTCTCTCA GCAGGGAGCG AGAGCTCCTG CAGAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCGTGGCGA CTGCGGAGGA CCAACATGGA GCTCCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAACAGCAGG AGGAGCTGCA CCTGGCCGTC 
CGAGAGAGGG AGCGTCTTCA GGAGACACTG GTGGTCCTGG AAGCCAGACA GTCCGAGTCC 
CTCAGTGAAC TGATCTGCCT TCGGGAAGCC CTGGAGTCCA GTCGCCTGGA AGGGGAGCTG 
CTGAGGCAGG AGCAAACAGA AGTGACCGCC GCGCTGGCCA GAGCAGAACA GTCAATCGTG 
GAGCTGTCAA GTGCTGAGAA CAGCTTGAAG GCTGAGGTCG CTGATCTTCG GGCTGCAGCT 
GTCAAACTGA GCGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGGA GAAGCAGTCT GTGTGCAGGA GGATGGAGGC AGCAGAGCAG 
GCGAGAAGCA CTTTGCAGGT GGACCTGGCG GAGGCCGAGA GGAGCAGGGA AGCCCTGTGG 
GAAAAGAACA GCCACCTGGA GGCCCAGCTG CAGAAAACAG AGGAGACCAG GGCGGAGCTG 
CAAGCGGACC TCAGGGGCAT CCAAGAAGAG AAGGAAGAAA TTCAAGAGAA GCTAAGCGAG 
ACATATCACC AGCAGGAGGC AGCTTCAGCT CAGATAGAAC AGCTAAAGCA AGAGACAACG 
CGCCAGGAAG AGATGCTCGC CCGAGAAGTC CAGGAGAAGG AGGCTCTGGC ACGGGAGAAG 
GCAGCTCTCG AGGTGCGGCT ACAGGCTGTG GAGCGAGACC GGCAGGAGCT CGCAGAACAA 
CTTCTGGGGC TCAGCTCAGC CAAGGAGGTA CTGGAGAGCA GTCTGTTTGA GGCCCAGCAA 
CAAAATTCTC TGACAGAGGT CACCAAAGGG CAGCTGGAGG TCCAGATTCA AACTGTCACG 
CAAGCCAAGG AAGTAATCCA AGGGGAAGTG AAGTGCCTGC AGCTGGAACT GGACACTGAA 
CGGAGCCGGG CAGAGCAGGC ACGGGACACA GCAGCCAGGC AGCTGGCCCA GGCTGAGCAA 
GAAGGGCAGG CTGCCCTGCA GCAGCTGAAG TCGGCCCATG AGGAGGAGGT GAATCGGCTC 
CAGGAGAAGT GGGAAGAAGA GCGCTCCCGG CACCGGCAGG AACTGGAGAA GGTTCTGGAG 
AGCCTGGAGA GGGAGAAGAT GGAGCTGGAA GCGAGGCTGA GGGAACAACA GGCAGAAATG 
GAGGCTATGC GGGCGCAGAG GGAGGAAGAG CGGGCCGAGG CCGAGAGCGC CCTGTGCCAG 
ATGCAGCTCG AGACAGAGAA GGAGAGGATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGAGGCCAG CCAACAGCTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
CAGTTAAAGG AGCAGGAGAC CACCGGGATG CTGCAGACCC AGCTCCAGGA GGCTCAGCAG 
GCACTGGAGC AGGCAGCCCA GCAGCACAGA GACAGTCTGG CGACCCTCCA GCAGGAGGGC 
CGAGTCCTGC TGCAGGATAA GACAGACCTG CAGAAGCAGG TGGAAGACTT GAAGTCTCAG 
CTGCTTTCCA AAGAAGCCTC CCAGAGGTTC GTGGACCAGG AGGTTCAGGA AAAGCTGAGA 
GAGGCCCAGG AGTGTAGCCG AATTCAGAAG GAGCTGGAGA GAGAAAAAGC CAGCCTGACT 
CTGTCGCTGG TGGAAAAGGA ACAGAGACTC CTTGTTCTGC AAGAGGCTGA CTCTGTTCAA 
CAGCAAGAGC TGAGCTCCCT GCGCCAGGAT ATGCAGGAGG CCCAGGCCGG GCAGAAAGAG 
CTCAGCACCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG AAAAGGAGGC TGACTTCCTG 
GCCCAGGAGA CTCAGCTGCT GGAGGAGCTT GAGGCGTCTC GAGTCACCGA ACAACAGCTC 
CGAGCTTCCT TGTGGGCCCA GGAGACCAAG GTGGCCCAAC TACAGCTGCG CCTGCGCAGC 
ACAGAGAGCC GGCTGGAGGC GCTGGTGGCG GAGCAGCAGC CTGGGCACCA GGCCCAGGTC 
CAGCTGGCCA GCCTCTATTC TGTCCTGCAG CAGGCCCTGG GGTCTGCCTG CGAGAGCAGG 
CTGGAGCTAA ATGGCGGGGG AGACTCAGCA CCCTCCCTCT GGGGCCCGGA GCCAGACCAG 
AATGGCGCTA GGATCCTCTT TAAGAGAGGG CCCCTCCTGA CGGCTCTCTC AGTTGAGGCA 
GTGGCGGCCG CCCTCCACAA GCTTCACCAA GACCTGTGGA AGACGCAGCA GGCCCGGGAT 
GATCTGCGGG ATCAAGCCCA GAAGCTGGAA CAGCGTCTCA GTGATATGGA AGCCGAGAAG 
AGCCAGGTCC ACACAGAGTT GCAGGCTCTG CAGAGACAGC TCTCCCAGAA TGAGGAAGAG 
AAATCCAAGT GGGAAGGCAA ACAGAACTCC CTAGAATCTG AGCTGATGGA ACTGCGTGAA 
ACTGTGGCAT CCTTACAGAG TCGCCTCCAG CAAGCAGAGC TGCAAGGAGT GGAAGCCCAG 
AATGAGCGCA AGTTACTGCA GGCAGCCAAG GAGAGCCTGA CTGCCCAGGT GGAACATCTC 
CAAGCCTCTA TCACAGAAGC CAGGGCTCAG GCAAGTGCTG CTGTGGCCCT GGAGGAAGAT 
CTGCGGATGG CGCGCTCAGC CCTGAAACTG AAAAATGAGG AGGTGGCGAG TGAGCGCCAG 
CGAGCCCAGG CCCTGCAGGA GCAGGGTGAG CTGAAAGTGG CCCAGGGGAA GGCTCTGCAG 
GAGAATCTGG CTCGCCTGGC CCAGGCCCTC TCGGAAAGAG AAGGGGAGGT GGAGACTCTG 
CAGGGGAAAA TTGAGGAACT GGAGAAGCAA CGGGAAATGC AGAAGGCTGT GTTGGAAGTG 
CTGTCTCTGG ACCTGAAGAA GAGGAACCGA GAGGTGGATC TACAGCAGGG ACAGATCCAG 
GAGCTGGAGG AGTGTAGGTC TGTGCTAGAG CATCTCCCCG TGGCTGTTCA GGAGCGAGAG 
CAGCAGCTGG CCCTGCAGAG AGAGCGGATC CGGGAACTTG AGAAGGACCG GGAGACGCAG 
ACGAGCATCC TAGAGCATCA TCTCCTGGAA CTTGAGAAGA AAGTCCAAGT GATCGAGTCC 
CAGAAAGGCC AGATTCAGGA CCTGAAGAAG CAGCTGGTTA CGACGGAATG CCTGGCCCTG 
GAGCTCGAAG AGAAGCGTCA CAAAACGGAG AGCCAGCAAA AGGCGATAGA GGAGCTGGAG 
GGCCAGAGGG AAGCACAGAG GGTGGCTCTG ACCCACCTAA CGCTGGACCT GGAGGAAAGG 
AGCCAGGAAC TGCTGGCACA GGGCAACCGG ATCCAGGAGC TGGAGGGCCA CAGCACTCTC 
CTGGCTAGTG AGCTTCAGGA GAAGGAGCAG GAGGCCATGG CCCAGCAAGA CCAGATCCGG 
GATCTGCAGA GGCAGAAAGA GCGTCTAAGT CAAGACCTGG AGAGGAGGGA GCAGGAGCTA 
GTGCTGCTGA AGGAGAGGAC GCAGTCCCTG GAAGATCAGA GGAGCCGGCA GACCAAGATC 
CTGGAGGAAG ACCTGGAGCA GATCAAGCTG GCCCTGAGAG AGCGGGGCCG GGAGCTGGTC 
TCGCAGCGGC AGCTGAGGCA GGAGTGGGCA GAGGAAGGGA AGGGCCAGAG CAAAGCTCAG 
CGTGGAAGCC TGGAGCACAT GAAGCTGATC CTGCGAGATA AGGAGAAGGA GGTGGAGTGC 
CAGCAGGAGC ACATCCAGGA GCTTCGGGAA CACAGGGACC AGCTGGAACA GGAGCTCCAG 
GGCCTTCACA GGAAGGTGAG TGAGACCAGC CTCCTTTTGA CCCAGCGGGA GCAGGAGATT 
GCGGTCCTGC AGCAGCACCT GCAGGAAGCC CAGGACCAGG GGGAGCTGAA AGAGCAGGAG 
CTTCAGGGGC AGCTGGAAGA GACCCAGAGG GTCCTGGTGC AGAGGACCCA GGAGCTCCAG 
GCCCTGCAGC AGCAAGAGGC CCAGGACCAG GAGAAGAACG TGAAGGAGCA GGCAGACTCA 
CTGCAGACCG CCCTGGAGCA GGCGCATGCT GCCCTGGAGG AGCGCCGGGC TGAGCTCGAG 
GAACACAAGG AACACGCGCG GAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCGGCGGAGC 
CAGGCCCTGG AAGAGGTGCT GGGTGACCTT CGGGCAGAGG CTCGGGAGCA GGAGAAGGTG 
CTGCGGGCCC TCCAGCAGCA GTGTGCCGAT CAGGCCCAGG AGCATGAGGC GGAGGCCCGG 
GGCCTGCGGG ACAGCTGGCT GCAGGCAGAG GCGCTGCTCG ACGCCCGTGA CAGGGAGCTG 
GAGGCCCTAC GGGCAGAGGG CCAGGCCTCC CAACGGCAGG AGGAGGCTGC CCGGGGCCAG 
GCTGAAGCTC TGCAGAAGGC CCTTGGCCAG GCTCAGGCCG CCCTGCGGGA GAAAGAGCAG 
CATCTTCATG GGCAGGCCGA ACTGAGCCGC AACCTGGAGG CCAGCACGGC CACCTTGCAG 
GCCGCCCTGG ACTCCTGCCA GGCCCAAGCC CGGCAGCTCG AGGAGACCCT GAGGAAGCGG 
GAAGGTGAGA TCCAAGACGG GGACCTCCGG CACCAGGAGA CCGTGCAGCA GCTCCAGCAG 
GCGCTTGCCC AGAGGGACAA AGAGCTGAGA GAGCTGAGGA GGCAGGGGCA GCTGCTAGAG 
AAGTCTCTGG CCCAAGAGAA GCAGGAGCGC AGGCACGAGA GAGAAGGGGA AGAGGTGAGG 
GGCCTCCGGG AGAGCCTCAG GGAGCTACAG CGGACCCTGG CTCAAAAGGA AGACGAGCTC 
TTGGAGCTGC AGGAGGCCCA GCAAAGGAAG GATCTGGAGG ACGCACCCCA CAGCCACAAA 
ACCTCGCCCA GGGAGGAGCC GTCTTCCCCA CTCGACTCCT CAGGGCCCAG GCTGCAGCGG 
GAGCTGGAGC GACTGCAGAG GGCTCTGAGG CAGACAGAGG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGCACCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGCGT GAGCAGTCTG 
CAGGAGGCGG CCCTGCTCCT ACAGGCCTCC GTGCTGGAGC GGGACTCGGA GCAGCAGAGG 
CTGCAGGACG AACTAGAGCT CACCAGACAA GCTCTGGAGA AGGAGCGACT CCACAGCCTG 
GGCCTGACCA GCCAAGCAGA ACAGGGGCCT GGAGGAGAGC CCGGCATCCG GTTGGGAGAG 
GTCTCAGAGG TGAAGATGGA GCCGAGTCCT GAGGCAGAGG AGAAGCAGCG CTGGGAGCAG 
AGGCTTGAAC ACCTGCAGCG AGCGGTGGCG CAGCTGGAGG TTGACCGGAG CCGGCTGCAG 
CACCACAACA CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGAGT CCATGCGCGC GTCGAGGGCA GGGGTCCTAG AGAGCAGTGG AGCCACAACA 
CCGTCCCCCA CACCCCAGGA TGGAAGAGGA GGACAGAAAG TCTCCTCGGA TGCCACGCAC 
ATGGCTGAGC TGCAGAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATCGC CCGCTCGGTG CAGACGAGCC GTGAGCTGGC GGGCCTGCAC 
CACAGCCTCT CTCACTCCCT CCTGGCCGTG GCCCAGGCCC CCGAGGCCAC CGTCCTGGAG 
GCCGAGACCC GCAAGCTGGA TGAGTCCCTG ACTCAAAGCC TGACATCGCC CGGGCCCATC 
CTGCTGTGCC GCAGCCCCAG CCCTGCTCAA GGCGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474285.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329771.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

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CloneID	OBa25632
Clone ID Related Accession (Same CDS sequence)	XM_015474285.1 , XM_015466168.1
Accession Version	XM_015474285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329771.1
CDS	117..7052
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474285.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474285.1

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015466168.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321654.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

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CloneID	OBa25632
Clone ID Related Accession (Same CDS sequence)	XM_015474285.1 , XM_015466168.1
Accession Version	XM_015466168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321654.1
CDS	117..7052
Translation	MEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015466168.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466168.1

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTC 
GACTGGAGCC GGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CACGGTCGCC TTCTGGGTCT ATGGCGGGAG 
GTGGTGACTT TCCGACGCCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGACG 
GAGCTCAAGG CTGAGCACGC GAGGCTTTCG GGGTCCCTGT TGACCTCTTG CCTGCGCCTG 
ACTGTGGGAG TACCACCATC TCGGGAGTCT GATGGATCTG GGAGACTGAA TGGAAACGAG 
CCATCCCAGT TGATGCTGCT ACTAGCCAAG ACCCAGGAAC TGGAGAGGGA AGCCCATGAA 
AGGAGCCAGG AGTTAATACA GCTGAAGAGT CAAGGGGATC TGGAGAAGGC AGAGCTTCAG 
GATCGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_010824119.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822421.2 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

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CloneID	OBa46857
Clone ID Related Accession (Same CDS sequence)	XM_010824119.2 , XM_010811675.2
Accession Version	XM_010824119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAATGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCCAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTGAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCAGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822421.2
CDS	211..7437
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGCRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_010824119.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010824119.2

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAATGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCCAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTGAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCAGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_010811675.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010809977.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

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CloneID	OBa46857
Clone ID Related Accession (Same CDS sequence)	XM_010824119.2 , XM_010811675.2
Accession Version	XM_010811675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAATGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCCAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTGAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCAGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010809977.1
CDS	211..7437
Translation	METGGPGLNNMKPRSLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQAALVRKLQAKVLQYRSWCQELEKRLEATGCRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_010811675.2
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811675.2

ORF Insert Sequence:

ATGGAGACAG GGGGCCCTGG GTTGAACAAT ATGAAGCCCC GGTCCCTGCA GCTGGTGCTG 
GAAGAGCAAG TGCTGGCACT GCAGCAGCAG ATGGCCGAGA ACCAGGCAGC CTCATGGAGG 
AAGCTGAAGA GCTCCCAGGA GGCCCAGCAG AGGCAAGCAG CTCTCGTGAG GAAGCTGCAG 
GCCAAGGTGT TACAGTACCG GAGCTGGTGC CAGGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGTGCAGGT GTGAGAATCT AGTGGAGGTG AACACCCAGC TTCGGCTGCA CATGGAAAAA 
GCTGACGTGG TGAATAAAGC GCTTCGGGAA GATGTGGAAA AATTGACAGT CGACTGGAGC 
CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG AGCCAGTGGC AGATGGAGCA GGAGTTCTTC 
AAGGGCTACC TGAAAGGGGA GCACGGTCGC CTTCTGGGTC TATGGCGGGA GGTGGTGACT 
TTCCGACGCC ACTTTCTGGA AATGAAGTCA GCCACTGACA GAGACCTGAC GGAGCTCAAG 
GCTGAGCACG CGAGGCTTTC GGGGTCCCTG TTGACCTCTT GCCTGCGCCT GACTGTGGGA 
GTACCACCAT CTCGGGAGTC TGATGGATCT GGGAGACTGA ATGGAAACGA GCCATCCCAG 
TTGATGCTGC TACTAGCCAA GACCCAGGAA CTGGAGAGGG AAGCCCATGA AAGGAGCCAG 
GAGTTAATAC AGCTGAAGAG TCAAGGGGAT CTGGAGAAGG CAGAGCTTCA GGATCGGGTG 
ACTGAGCTCT CTGCTCTGCT GACCCAGTCT CAGAAGCAAA ATGAAGATTA TGAAAAGATG 
GTGAAGGCTC TGAGAGCGAC GATGGAGATC CTGGAGGCAA ATCATGCAGA ATTAATGGAA 
CACGAGGCAT CTCTTAGTAG GAACGCCCAA GAGGAGAAGT TGTCTTTACA GCAAGTGATC 
CAGGATATAA CCCAGGCCAT GGTAGAAGAA GAGGACAGTA TGGCCCAAGG TTCTAGTCAC 
GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC TTCTCTTCCC GGTTTGATTC CCAGCAGCCA 
GACAAGGCTG TCTCTCTGGT GCGTTCGGTA CTGACTCAGA GACGCCAGGC TGTGCAGGAC 
CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA GCTGTGAGCT CCTGGCAACA GCAGCATCAC 
CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG 
GACACCCTGG CGGGGCAGAC GGTGGACCTG CAGGGAGAGG TGGACTCTCT CAGCAGGGAG 
CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG 
GAGGCGTGGC GACTGCGGAG GACCAACATG GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG 
GGCGAGAAGG AGGAACAGCA GGAGGAGCTG CACCTGGCCG TCCGAGAGAG GGAGCGTCTT 
CAGGAGACAC TGGTGGTCCT GGAAGCCAGA CAGTCCGAGT CCCTCAGTGA ACTGATCTGC 
CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG GAAGGGGAGC TGCTGAGGCA GGAGCAAACA 
GAAGTGACCG CCGCGCTGGC CAGAGCAGAA CAGTCAATCG TGGAGCTGTC AAGTGCTGAG 
AACAGCTTGA AGGCTGAGGT CGCTGATCTT CGGGCTGCAG CTGTCAAACT GAGCGCCTTA 
AATGAGGCTT TGGCCTTAGA TAAAGTTGGA CTGAACCAGC AGCTTCTCCA GTTAGAACAG 
GAGAAGCAGT CTGTGTGCAG GAGGATGGAG GCAGCAGAGC AGGCGAGAAG CACTTTGCAG 
GTGGACCTGG CGGAGGCCGA GAGGAGCAGG GAAGCCCTGT GGGAAAAGAA CAGCCACCTG 
GAGGCCCAGC TGCAGAAAAC AGAGGAGACC AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC 
ATCCAAGAAG AGAAGGAAGA AATTCAAGAG AAGCTAAGCG AGACATATCA CCAGCAGGAG 
GCAGCTTCAG CTCAGATAGA ACAGCTAAAG CAAGAGACAA CGCGCCAGGA AGAGATGCTC 
GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG GCACGGGAGA AGGCAGCTCT CGAGGTGCGG 
CTACAGGCTG TGGAGCGAGA CCGGCAGGAG CTCGCAGAAC AACTTCTGGG GCTCAGCTCA 
GCCAAGGAGG TACTGGAGAG CAGTCTGTTT GAGGCCCAGC AACAAAATTC TCTGACAGAG 
GTCACCAAAG GGCAGCTGGA GGTCCAGATT CAAACTGTCA CGCAAGCCAA GGAAGTAATC 
CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA CTGGACACTG AACGGAGCCG GGCAGAGCAG 
GCACGGGACA CAGCAGCCAG GCAGCTGGCC CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG 
CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG GTGAATCGGC TCCAGGAGAA GTGGGAAGAA 
GAGCGCTCCC GGCACCGGCA GGAACTGGAG AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG 
ATGGAGCTGG AAGCGAGGCT GAGGGAACAA CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG 
AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC GCCCTGTGCC AGATGCAGCT CGAGACAGAG 
AAGGAGAGGA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGAGGCC 
AGCCAACAGC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG 
ACCACCGGGA TGCTGCAGAC CCAGCTCCAG GAGGCTCAGC AGGCACTGGA GCAGGCAGCC 
CAGCAGCACA GAGACAGTCT GGCGACCCTC CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT 
AAGACAGACC TGCAGAAGCA GGTGGAAGAC TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC 
TCCCAGAGGT TCGTGGACCA GGAGGTTCAG GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC 
CGAATTCAGA AGGAGCTGGA GAGAGAAAAA GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGTTCT GCAAGAGGCT GACTCTGTTC AACAGCAAGA GCTGAGCTCC 
CTGCGCCAGG ATATGCAGGA GGCCCAGGCC GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGAAAAGGAG GCTGACTTCC TGGCCCAGGA GACTCAGCTG 
CTGGAGGAGC TTGAGGCGTC TCGAGTCACC GAACAACAGC TCCGAGCTTC CTTGTGGGCC 
CAGGAGACCA AGGTGGCCCA ACTACAGCTG CGCCTGCGCA GCACAGAGAG CCGGCTGGAG 
GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT 
TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC TGCGAGAGCA GGCTGGAGCT AAATGGCGGG 
GGAGACTCAG CACCCTCCCT CTGGGGCCCG GAGCCAGACC AGAATGGCGC TAGGATCCTC 
TTTAAGAGAG GGCCCCTCCT GACGGCTCTC TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC 
AAGCTTCACC AAGACCTGTG GAAGACGCAG CAGGCCCGGG ATGATCTGCG GGATCAAGCC 
CAGAAGCTGG AACAGCGTCT CAGTGATATG GAAGCCGAGA AGAGCCAGGT CCACACAGAG 
TTGCAGGCTC TGCAGAGACA GCTCTCCCAG AATGAGGAAG AGAAATCCAA GTGGGAAGGC 
AAACAGAACT CCCTAGAATC TGAGCTGATG GAACTGCGTG AAACTGTGGC ATCCTTACAG 
AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA GTGGAAGCCC AGAATGAGCG CAAGTTACTG 
CAGGCAGCCA AGGAGAGCCT GACTGCCCAG GTGGAACATC TCCAAGCCTC TATCACAGAA 
GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA 
GCCCTGAAAC TGAAAAATGA GGAGGTGGCG AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG 
GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG AAGGCTCTGC AGGAGAATCT GGCTCGCCTG 
GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG GTGGAGACTC TGCAGGGGAA AATTGAGGAA 
CTGGAGAAGC AACGGGAAAT GCAGAAGGCT GTGTTGGAAG TGCTGTCTCT GGACCTGAAG 
AAGAGGAACC GAGAGGTGGA TCTACAGCAG GGACAGATCC AGGAGCTGGA GGAGTGTAGG 
TCTGTGCTAG AGCATCTCCC CGTGGCTGTT CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG 
AGAGAGCGGA TCCGGGAACT TGAGAAGGAC CGGGAGACGC AGACGAGCAT CCTAGAGCAT 
CATCTCCTGG AACTTGAGAA GAAAGTCCAA GTGATCGAGT CCCAGAAAGG CCAGATTCAG 
GACCTGAAGA AGCAGCTGGT TACGACGGAA TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT 
CACAAAACGG AGAGCCAGCA AAAGGCGATA GAGGAGCTGG AGGGCCAGAG GGAAGCACAG 
AGGGTGGCTC TGACCCACCT AACGCTGGAC CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA 
CAGGGCAACC GGATCCAGGA GCTGGAGGGC CACAGCACTC TCCTGGCTAG TGAGCTTCAG 
GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA GACCAGATCC GGGATCTGCA GAGGCAGAAA 
GAGCGTCTAA GTCAAGACCT GGAGAGGAGG GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG 
ACGCAGTCCC TGGAAGATCA GAGGAGCCGG CAGACCAAGA TCCTGGAGGA AGACCTGGAG 
CAGATCAAGC TGGCCCTGAG AGAGCGGGGC CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG 
CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC 
ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG GAGGTGGAGT GCCAGCAGGA GCACATCCAG 
GAGCTTCGGG AACACAGGGA CCAGCTGGAA CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG 
AGTGAGACCA GCCTCCTTTT GACCCAGCGG GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC 
CTGCAGGAAG CCCAGGACCA GGGGGAGCTG AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA 
GAGACCCAGA GGGTCCTGGT GCAGAGGACC CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG 
GCCCAGGACC AGGAGAAGAA CGTGAAGGAG CAGGCAGACT CACTGCAGAC CGCCCTGGAG 
CAGGCGCATG CTGCCCTGGA GGAGCGCCGG GCTGAGCTCG AGGAACACAA GGAACACGCG 
CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG 
CTGGGTGACC TTCGGGCAGA GGCTCGGGAG CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG 
CAGTGTGCCG ATCAGGCCCA GGAGCATGAG GCGGAGGCCC GGGGCCTGCG GGACAGCTGG 
CTGCAGGCAG AGGCGCTGCT CGACGCCCGT GACAGGGAGC TGGAGGCCCT ACGGGCAGAG 
GGCCAGGCCT CCCAACGGCA GGAGGAGGCT GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG 
GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG GAGAAAGAGC AGCATCTTCA TGGGCAGGCC 
GAACTGAGCC GCAACCTGGA GGCCAGCACG GCCACCTTGC AGGCCGCCCT GGACTCCTGC 
CAGGCCCAAG CCCGGCAGCT CGAGGAGACC CTGAGGAAGC GGGAAGGTGA GATCCAAGAC 
GGGGACCTCC GGCACCAGGA GACCGTGCAG CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC 
AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG 
AAGCAGGAGC GCAGGCACGA GAGAGAAGGG GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC 
AGGGAGCTAC AGCGGACCCT GGCTCAAAAG GAAGACGAGC TCTTGGAGCT GCAGGAGGCC 
CAGCAAAGGA AGGATCTGGA GGACGCACCC CACAGCCACA AAACCTCGCC CAGGGAGGAG 
CCGTCTTCCC CACTCGACTC CTCAGGGCCC AGGCTGCAGC GGGAGCTGGA GCGACTGCAG 
AGGGCTCTGA GGCAGACAGA GGCCAGGGAG ATCGAGTGGA GGGAGAAGGC CCAGCACCTG 
GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC 
CTACAGGCCT CCGTGCTGGA GCGGGACTCG GAGCAGCAGA GGCTGCAGGA CGAACTAGAG 
CTCACCAGAC AAGCTCTGGA GAAGGAGCGA CTCCACAGCC TGGGCCTGAC CAGCCAAGCA 
GAACAGGGGC CTGGAGGAGA GCCCGGCATC CGGTTGGGAG AGGTCTCAGA GGTGAAGATG 
GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG CGCTGGGAGC AGAGGCTTGA ACACCTGCAG 
CGAGCGGTGG CGCAGCTGGA GGTTGACCGG AGCCGGCTGC AGCACCACAA CACCCAGCTG 
CGGACCACCC TGGAGCAGGT GGAGCGAGAG CGGAGGAAAC TGAAGAGGGA GTCCATGCGC 
GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT GGAGCCACAA CACCGTCCCC CACACCCCAG 
GATGGAAGAG GAGGACAGAA AGTCTCCTCG GATGCCACGC ACATGGCTGA GCTGCAGAAA 
GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC TTGGAGCGGA AGCAGAAGCA GGACTACATC 
GCCCGCTCGG TGCAGACGAG CCGTGAGCTG GCGGGCCTGC ACCACAGCCT CTCTCACTCC 
CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG 
GATGAGTCCC TGACTCAAAG CCTGACATCG CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC 
AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015466167.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321653.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

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CloneID	OBa92412
Clone ID Related Accession (Same CDS sequence)	XM_015466167.1 , XM_015474284.1
Accession Version	XM_015466167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAATGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCCAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTGAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCAGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321653.1
CDS	98..7294
Translation	MEEAEELPGGPAEASSSREEAAGQGVTVPELVPGAGEAAGSHWGTDPSKVGKRGKAKPGTAANPIGRGTTECRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015466167.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466167.1

ORF Insert Sequence:

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAATGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCCAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTGAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCAGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474284.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329770.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

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CloneID	OBa92412
Clone ID Related Accession (Same CDS sequence)	XM_015466167.1 , XM_015474284.1
Accession Version	XM_015474284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAATGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCCAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTGAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCAGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329770.1
CDS	98..7294
Translation	MEEAEELPGGPAEASSSREEAAGQGVTVPELVPGAGEAAGSHWGTDPSKVGKRGKAKPGTAANPIGRGTTECRCENLVEVNTQLRLHMEKADVVNKALREDVEKLTVDWSRARDELMRKESQWQMEQEFFKGYLKGEHGRLLGLWREVVTFRRHFLEMKSATDRDLTELKAEHARLSGSLLTSCLRLTVGVPPSRESDGSGRLNGNEPSQLMLLLAKTQELEREAHERSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474284.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474284.1

ORF Insert Sequence:

ATGGAGGAAG CTGAAGAGCT CCCAGGAGGC CCAGCAGAGG CAAGCAGCTC TCGTGAGGAA 
GCTGCAGGCC AAGGTGTTAC AGTACCGGAG CTGGTGCCAG GAGCTGGAGA AGCGGCTGGA 
AGCCACTGGG GGACCGATCC CTCAAAGGTG GGAAAGCGTG GAAAAGCCAA ACCTGGAACA 
GCTGCTAATC CGATTGGAAG AGGAACAACA GAGTGCAGGT GTGAGAATCT AGTGGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT CGACTGGAGC CGGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCACGGTCGC 
CTTCTGGGTC TATGGCGGGA GGTGGTGACT TTCCGACGCC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAC GGAGCTCAAG GCTGAGCACG CGAGGCTTTC GGGGTCCCTG 
TTGACCTCTT GCCTGCGCCT GACTGTGGGA GTACCACCAT CTCGGGAGTC TGATGGATCT 
GGGAGACTGA ATGGAAACGA GCCATCCCAG TTGATGCTGC TACTAGCCAA GACCCAGGAA 
CTGGAGAGGG AAGCCCATGA AAGGAGCCAG GAGTTAATAC AGCTGAAGAG TCAAGGGGAT 
CTGGAGAAGG CAGAGCTTCA GGATCGGGTG ACTGAGCTCT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAAA ATGAAGATTA TGAAAAGATG GTGAAGGCTC TGAGAGCGAC GATGGAGATC 
CTGGAGGCAA ATCATGCAGA ATTAATGGAA CACGAGGCAT CTCTTAGTAG GAACGCCCAA 
GAGGAGAAGT TGTCTTTACA GCAAGTGATC CAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GAGGACAGTA TGGCCCAAGG TTCTAGTCAC GAGAGCTCCT TGCAGTTGGA CCCTGGTGGC 
TTCTCTTCCC GGTTTGATTC CCAGCAGCCA GACAAGGCTG TCTCTCTGGT GCGTTCGGTA 
CTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAGGCAGC AGCTGTCAGG CTGCCAGGAA 
GCTGTGAGCT CCTGGCAACA GCAGCATCAC CAGTGGGAGG AAGAGGGTGA AGCCCTGAGA 
CAGCGGCTGC AGCAGCTCAC AGGGGAGCGG GACACCCTGG CGGGGCAGAC GGTGGACCTG 
CAGGGAGAGG TGGACTCTCT CAGCAGGGAG CGAGAGCTCC TGCAGAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCGTGGC GACTGCGGAG GACCAACATG 
GAGCTCCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAACAGCA GGAGGAGCTG 
CACCTGGCCG TCCGAGAGAG GGAGCGTCTT CAGGAGACAC TGGTGGTCCT GGAAGCCAGA 
CAGTCCGAGT CCCTCAGTGA ACTGATCTGC CTTCGGGAAG CCCTGGAGTC CAGTCGCCTG 
GAAGGGGAGC TGCTGAGGCA GGAGCAAACA GAAGTGACCG CCGCGCTGGC CAGAGCAGAA 
CAGTCAATCG TGGAGCTGTC AAGTGCTGAG AACAGCTTGA AGGCTGAGGT CGCTGATCTT 
CGGGCTGCAG CTGTCAAACT GAGCGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG GAGAAGCAGT CTGTGTGCAG GAGGATGGAG 
GCAGCAGAGC AGGCGAGAAG CACTTTGCAG GTGGACCTGG CGGAGGCCGA GAGGAGCAGG 
GAAGCCCTGT GGGAAAAGAA CAGCCACCTG GAGGCCCAGC TGCAGAAAAC AGAGGAGACC 
AGGGCGGAGC TGCAAGCGGA CCTCAGGGGC ATCCAAGAAG AGAAGGAAGA AATTCAAGAG 
AAGCTAAGCG AGACATATCA CCAGCAGGAG GCAGCTTCAG CTCAGATAGA ACAGCTAAAG 
CAAGAGACAA CGCGCCAGGA AGAGATGCTC GCCCGAGAAG TCCAGGAGAA GGAGGCTCTG 
GCACGGGAGA AGGCAGCTCT CGAGGTGCGG CTACAGGCTG TGGAGCGAGA CCGGCAGGAG 
CTCGCAGAAC AACTTCTGGG GCTCAGCTCA GCCAAGGAGG TACTGGAGAG CAGTCTGTTT 
GAGGCCCAGC AACAAAATTC TCTGACAGAG GTCACCAAAG GGCAGCTGGA GGTCCAGATT 
CAAACTGTCA CGCAAGCCAA GGAAGTAATC CAAGGGGAAG TGAAGTGCCT GCAGCTGGAA 
CTGGACACTG AACGGAGCCG GGCAGAGCAG GCACGGGACA CAGCAGCCAG GCAGCTGGCC 
CAGGCTGAGC AAGAAGGGCA GGCTGCCCTG CAGCAGCTGA AGTCGGCCCA TGAGGAGGAG 
GTGAATCGGC TCCAGGAGAA GTGGGAAGAA GAGCGCTCCC GGCACCGGCA GGAACTGGAG 
AAGGTTCTGG AGAGCCTGGA GAGGGAGAAG ATGGAGCTGG AAGCGAGGCT GAGGGAACAA 
CAGGCAGAAA TGGAGGCTAT GCGGGCGCAG AGGGAGGAAG AGCGGGCCGA GGCCGAGAGC 
GCCCTGTGCC AGATGCAGCT CGAGACAGAG AAGGAGAGGA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGAGGCC AGCCAACAGC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGCAGTTAAA GGAGCAGGAG ACCACCGGGA TGCTGCAGAC CCAGCTCCAG 
GAGGCTCAGC AGGCACTGGA GCAGGCAGCC CAGCAGCACA GAGACAGTCT GGCGACCCTC 
CAGCAGGAGG GCCGAGTCCT GCTGCAGGAT AAGACAGACC TGCAGAAGCA GGTGGAAGAC 
TTGAAGTCTC AGCTGCTTTC CAAAGAAGCC TCCCAGAGGT TCGTGGACCA GGAGGTTCAG 
GAAAAGCTGA GAGAGGCCCA GGAGTGTAGC CGAATTCAGA AGGAGCTGGA GAGAGAAAAA 
GCCAGCCTGA CTCTGTCGCT GGTGGAAAAG GAACAGAGAC TCCTTGTTCT GCAAGAGGCT 
GACTCTGTTC AACAGCAAGA GCTGAGCTCC CTGCGCCAGG ATATGCAGGA GGCCCAGGCC 
GGGCAGAAAG AGCTCAGCAC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGAAAAGGAG 
GCTGACTTCC TGGCCCAGGA GACTCAGCTG CTGGAGGAGC TTGAGGCGTC TCGAGTCACC 
GAACAACAGC TCCGAGCTTC CTTGTGGGCC CAGGAGACCA AGGTGGCCCA ACTACAGCTG 
CGCCTGCGCA GCACAGAGAG CCGGCTGGAG GCGCTGGTGG CGGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG TCCAGCTGGC CAGCCTCTAT TCTGTCCTGC AGCAGGCCCT GGGGTCTGCC 
TGCGAGAGCA GGCTGGAGCT AAATGGCGGG GGAGACTCAG CACCCTCCCT CTGGGGCCCG 
GAGCCAGACC AGAATGGCGC TAGGATCCTC TTTAAGAGAG GGCCCCTCCT GACGGCTCTC 
TCAGTTGAGG CAGTGGCGGC CGCCCTCCAC AAGCTTCACC AAGACCTGTG GAAGACGCAG 
CAGGCCCGGG ATGATCTGCG GGATCAAGCC CAGAAGCTGG AACAGCGTCT CAGTGATATG 
GAAGCCGAGA AGAGCCAGGT CCACACAGAG TTGCAGGCTC TGCAGAGACA GCTCTCCCAG 
AATGAGGAAG AGAAATCCAA GTGGGAAGGC AAACAGAACT CCCTAGAATC TGAGCTGATG 
GAACTGCGTG AAACTGTGGC ATCCTTACAG AGTCGCCTCC AGCAAGCAGA GCTGCAAGGA 
GTGGAAGCCC AGAATGAGCG CAAGTTACTG CAGGCAGCCA AGGAGAGCCT GACTGCCCAG 
GTGGAACATC TCCAAGCCTC TATCACAGAA GCCAGGGCTC AGGCAAGTGC TGCTGTGGCC 
CTGGAGGAAG ATCTGCGGAT GGCGCGCTCA GCCCTGAAAC TGAAAAATGA GGAGGTGGCG 
AGTGAGCGCC AGCGAGCCCA GGCCCTGCAG GAGCAGGGTG AGCTGAAAGT GGCCCAGGGG 
AAGGCTCTGC AGGAGAATCT GGCTCGCCTG GCCCAGGCCC TCTCGGAAAG AGAAGGGGAG 
GTGGAGACTC TGCAGGGGAA AATTGAGGAA CTGGAGAAGC AACGGGAAAT GCAGAAGGCT 
GTGTTGGAAG TGCTGTCTCT GGACCTGAAG AAGAGGAACC GAGAGGTGGA TCTACAGCAG 
GGACAGATCC AGGAGCTGGA GGAGTGTAGG TCTGTGCTAG AGCATCTCCC CGTGGCTGTT 
CAGGAGCGAG AGCAGCAGCT GGCCCTGCAG AGAGAGCGGA TCCGGGAACT TGAGAAGGAC 
CGGGAGACGC AGACGAGCAT CCTAGAGCAT CATCTCCTGG AACTTGAGAA GAAAGTCCAA 
GTGATCGAGT CCCAGAAAGG CCAGATTCAG GACCTGAAGA AGCAGCTGGT TACGACGGAA 
TGCCTGGCCC TGGAGCTCGA AGAGAAGCGT CACAAAACGG AGAGCCAGCA AAAGGCGATA 
GAGGAGCTGG AGGGCCAGAG GGAAGCACAG AGGGTGGCTC TGACCCACCT AACGCTGGAC 
CTGGAGGAAA GGAGCCAGGA ACTGCTGGCA CAGGGCAACC GGATCCAGGA GCTGGAGGGC 
CACAGCACTC TCCTGGCTAG TGAGCTTCAG GAGAAGGAGC AGGAGGCCAT GGCCCAGCAA 
GACCAGATCC GGGATCTGCA GAGGCAGAAA GAGCGTCTAA GTCAAGACCT GGAGAGGAGG 
GAGCAGGAGC TAGTGCTGCT GAAGGAGAGG ACGCAGTCCC TGGAAGATCA GAGGAGCCGG 
CAGACCAAGA TCCTGGAGGA AGACCTGGAG CAGATCAAGC TGGCCCTGAG AGAGCGGGGC 
CGGGAGCTGG TCTCGCAGCG GCAGCTGAGG CAGGAGTGGG CAGAGGAAGG GAAGGGCCAG 
AGCAAAGCTC AGCGTGGAAG CCTGGAGCAC ATGAAGCTGA TCCTGCGAGA TAAGGAGAAG 
GAGGTGGAGT GCCAGCAGGA GCACATCCAG GAGCTTCGGG AACACAGGGA CCAGCTGGAA 
CAGGAGCTCC AGGGCCTTCA CAGGAAGGTG AGTGAGACCA GCCTCCTTTT GACCCAGCGG 
GAGCAGGAGA TTGCGGTCCT GCAGCAGCAC CTGCAGGAAG CCCAGGACCA GGGGGAGCTG 
AAAGAGCAGG AGCTTCAGGG GCAGCTGGAA GAGACCCAGA GGGTCCTGGT GCAGAGGACC 
CAGGAGCTCC AGGCCCTGCA GCAGCAAGAG GCCCAGGACC AGGAGAAGAA CGTGAAGGAG 
CAGGCAGACT CACTGCAGAC CGCCCTGGAG CAGGCGCATG CTGCCCTGGA GGAGCGCCGG 
GCTGAGCTCG AGGAACACAA GGAACACGCG CGGAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCGGCGGA GCCAGGCCCT GGAAGAGGTG CTGGGTGACC TTCGGGCAGA GGCTCGGGAG 
CAGGAGAAGG TGCTGCGGGC CCTCCAGCAG CAGTGTGCCG ATCAGGCCCA GGAGCATGAG 
GCGGAGGCCC GGGGCCTGCG GGACAGCTGG CTGCAGGCAG AGGCGCTGCT CGACGCCCGT 
GACAGGGAGC TGGAGGCCCT ACGGGCAGAG GGCCAGGCCT CCCAACGGCA GGAGGAGGCT 
GCCCGGGGCC AGGCTGAAGC TCTGCAGAAG GCCCTTGGCC AGGCTCAGGC CGCCCTGCGG 
GAGAAAGAGC AGCATCTTCA TGGGCAGGCC GAACTGAGCC GCAACCTGGA GGCCAGCACG 
GCCACCTTGC AGGCCGCCCT GGACTCCTGC CAGGCCCAAG CCCGGCAGCT CGAGGAGACC 
CTGAGGAAGC GGGAAGGTGA GATCCAAGAC GGGGACCTCC GGCACCAGGA GACCGTGCAG 
CAGCTCCAGC AGGCGCTTGC CCAGAGGGAC AAAGAGCTGA GAGAGCTGAG GAGGCAGGGG 
CAGCTGCTAG AGAAGTCTCT GGCCCAAGAG AAGCAGGAGC GCAGGCACGA GAGAGAAGGG 
GAAGAGGTGA GGGGCCTCCG GGAGAGCCTC AGGGAGCTAC AGCGGACCCT GGCTCAAAAG 
GAAGACGAGC TCTTGGAGCT GCAGGAGGCC CAGCAAAGGA AGGATCTGGA GGACGCACCC 
CACAGCCACA AAACCTCGCC CAGGGAGGAG CCGTCTTCCC CACTCGACTC CTCAGGGCCC 
AGGCTGCAGC GGGAGCTGGA GCGACTGCAG AGGGCTCTGA GGCAGACAGA GGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGCACCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGC 
GTGAGCAGTC TGCAGGAGGC GGCCCTGCTC CTACAGGCCT CCGTGCTGGA GCGGGACTCG 
GAGCAGCAGA GGCTGCAGGA CGAACTAGAG CTCACCAGAC AAGCTCTGGA GAAGGAGCGA 
CTCCACAGCC TGGGCCTGAC CAGCCAAGCA GAACAGGGGC CTGGAGGAGA GCCCGGCATC 
CGGTTGGGAG AGGTCTCAGA GGTGAAGATG GAGCCGAGTC CTGAGGCAGA GGAGAAGCAG 
CGCTGGGAGC AGAGGCTTGA ACACCTGCAG CGAGCGGTGG CGCAGCTGGA GGTTGACCGG 
AGCCGGCTGC AGCACCACAA CACCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGA GTCCATGCGC GCGTCGAGGG CAGGGGTCCT AGAGAGCAGT 
GGAGCCACAA CACCGTCCCC CACACCCCAG GATGGAAGAG GAGGACAGAA AGTCTCCTCG 
GATGCCACGC ACATGGCTGA GCTGCAGAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATC GCCCGCTCGG TGCAGACGAG CCGTGAGCTG 
GCGGGCCTGC ACCACAGCCT CTCTCACTCC CTCCTGGCCG TGGCCCAGGC CCCCGAGGCC 
ACCGTCCTGG AGGCCGAGAC CCGCAAGCTG GATGAGTCCC TGACTCAAAG CCTGACATCG 
CCCGGGCCCA TCCTGCTGTG CCGCAGCCCC AGCCCTGCTC AAGGCGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474286.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329772.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

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CloneID	OBa92413
Clone ID Related Accession (Same CDS sequence)	XM_015474286.1 , XM_015466170.1
Accession Version	XM_015474286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329772.1
CDS	79..6534
Translation	MWVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474286.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474286.1

ORF Insert Sequence:

ATGTGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015466170.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321656.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

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CloneID	OBa92413
Clone ID Related Accession (Same CDS sequence)	XM_015474286.1 , XM_015466170.1
Accession Version	XM_015466170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321656.1
CDS	79..6534
Translation	MWVTELSALLTQSQKQNEDYEKMVKALRATMEILEANHAELMEHEASLSRNAQEEKLSLQQVIQDITQAMVEEEDSMAQGSSHESSLQLDPGGFSSRFDSQQPDKAVSLVRSVLTQRRQAVQDLRQQLSGCQEAVSSWQQQHHQWEEEGEALRQRLQQLTGERDTLAGQTVDLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQETLVVLEARQSESLSELICLREALESSRLEGELLRQEQTEVTAALARAEQSIVELSSAENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQEKQSVCRRMEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015466170.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466170.1

ORF Insert Sequence:

ATGTGGGTGA CTGAGCTCTC TGCTCTGCTG ACCCAGTCTC AGAAGCAAAA TGAAGATTAT 
GAAAAGATGG TGAAGGCTCT GAGAGCGACG ATGGAGATCC TGGAGGCAAA TCATGCAGAA 
TTAATGGAAC ACGAGGCATC TCTTAGTAGG AACGCCCAAG AGGAGAAGTT GTCTTTACAG 
CAAGTGATCC AGGATATAAC CCAGGCCATG GTAGAAGAAG AGGACAGTAT GGCCCAAGGT 
TCTAGTCACG AGAGCTCCTT GCAGTTGGAC CCTGGTGGCT TCTCTTCCCG GTTTGATTCC 
CAGCAGCCAG ACAAGGCTGT CTCTCTGGTG CGTTCGGTAC TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAGGCAGCA GCTGTCAGGC TGCCAGGAAG CTGTGAGCTC CTGGCAACAG 
CAGCATCACC AGTGGGAGGA AGAGGGTGAA GCCCTGAGAC AGCGGCTGCA GCAGCTCACA 
GGGGAGCGGG ACACCCTGGC GGGGCAGACG GTGGACCTGC AGGGAGAGGT GGACTCTCTC 
AGCAGGGAGC GAGAGCTCCT GCAGAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCGTGGCG ACTGCGGAGG ACCAACATGG AGCTCCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAACAGCAG GAGGAGCTGC ACCTGGCCGT CCGAGAGAGG 
GAGCGTCTTC AGGAGACACT GGTGGTCCTG GAAGCCAGAC AGTCCGAGTC CCTCAGTGAA 
CTGATCTGCC TTCGGGAAGC CCTGGAGTCC AGTCGCCTGG AAGGGGAGCT GCTGAGGCAG 
GAGCAAACAG AAGTGACCGC CGCGCTGGCC AGAGCAGAAC AGTCAATCGT GGAGCTGTCA 
AGTGCTGAGA ACAGCTTGAA GGCTGAGGTC GCTGATCTTC GGGCTGCAGC TGTCAAACTG 
AGCGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGG AGAAGCAGTC TGTGTGCAGG AGGATGGAGG CAGCAGAGCA GGCGAGAAGC 
ACTTTGCAGG TGGACCTGGC GGAGGCCGAG AGGAGCAGGG AAGCCCTGTG GGAAAAGAAC 
AGCCACCTGG AGGCCCAGCT GCAGAAAACA GAGGAGACCA GGGCGGAGCT GCAAGCGGAC 
CTCAGGGGCA TCCAAGAAGA GAAGGAAGAA ATTCAAGAGA AGCTAAGCGA GACATATCAC 
CAGCAGGAGG CAGCTTCAGC TCAGATAGAA CAGCTAAAGC AAGAGACAAC GCGCCAGGAA 
GAGATGCTCG CCCGAGAAGT CCAGGAGAAG GAGGCTCTGG CACGGGAGAA GGCAGCTCTC 
GAGGTGCGGC TACAGGCTGT GGAGCGAGAC CGGCAGGAGC TCGCAGAACA ACTTCTGGGG 
CTCAGCTCAG CCAAGGAGGT ACTGGAGAGC AGTCTGTTTG AGGCCCAGCA ACAAAATTCT 
CTGACAGAGG TCACCAAAGG GCAGCTGGAG GTCCAGATTC AAACTGTCAC GCAAGCCAAG 
GAAGTAATCC AAGGGGAAGT GAAGTGCCTG CAGCTGGAAC TGGACACTGA ACGGAGCCGG 
GCAGAGCAGG CACGGGACAC AGCAGCCAGG CAGCTGGCCC AGGCTGAGCA AGAAGGGCAG 
GCTGCCCTGC AGCAGCTGAA GTCGGCCCAT GAGGAGGAGG TGAATCGGCT CCAGGAGAAG 
TGGGAAGAAG AGCGCTCCCG GCACCGGCAG GAACTGGAGA AGGTTCTGGA GAGCCTGGAG 
AGGGAGAAGA TGGAGCTGGA AGCGAGGCTG AGGGAACAAC AGGCAGAAAT GGAGGCTATG 
CGGGCGCAGA GGGAGGAAGA GCGGGCCGAG GCCGAGAGCG CCCTGTGCCA GATGCAGCTC 
GAGACAGAGA AGGAGAGGAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGAGGCCA GCCAACAGCT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GCAGTTAAAG 
GAGCAGGAGA CCACCGGGAT GCTGCAGACC CAGCTCCAGG AGGCTCAGCA GGCACTGGAG 
CAGGCAGCCC AGCAGCACAG AGACAGTCTG GCGACCCTCC AGCAGGAGGG CCGAGTCCTG 
CTGCAGGATA AGACAGACCT GCAGAAGCAG GTGGAAGACT TGAAGTCTCA GCTGCTTTCC 
AAAGAAGCCT CCCAGAGGTT CGTGGACCAG GAGGTTCAGG AAAAGCTGAG AGAGGCCCAG 
GAGTGTAGCC GAATTCAGAA GGAGCTGGAG AGAGAAAAAG CCAGCCTGAC TCTGTCGCTG 
GTGGAAAAGG AACAGAGACT CCTTGTTCTG CAAGAGGCTG ACTCTGTTCA ACAGCAAGAG 
CTGAGCTCCC TGCGCCAGGA TATGCAGGAG GCCCAGGCCG GGCAGAAAGA GCTCAGCACC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GAAAAGGAGG CTGACTTCCT GGCCCAGGAG 
ACTCAGCTGC TGGAGGAGCT TGAGGCGTCT CGAGTCACCG AACAACAGCT CCGAGCTTCC 
TTGTGGGCCC AGGAGACCAA GGTGGCCCAA CTACAGCTGC GCCTGCGCAG CACAGAGAGC 
CGGCTGGAGG CGCTGGTGGC GGAGCAGCAG CCTGGGCACC AGGCCCAGGT CCAGCTGGCC 
AGCCTCTATT CTGTCCTGCA GCAGGCCCTG GGGTCTGCCT GCGAGAGCAG GCTGGAGCTA 
AATGGCGGGG GAGACTCAGC ACCCTCCCTC TGGGGCCCGG AGCCAGACCA GAATGGCGCT 
AGGATCCTCT TTAAGAGAGG GCCCCTCCTG ACGGCTCTCT CAGTTGAGGC AGTGGCGGCC 
GCCCTCCACA AGCTTCACCA AGACCTGTGG AAGACGCAGC AGGCCCGGGA TGATCTGCGG 
GATCAAGCCC AGAAGCTGGA ACAGCGTCTC AGTGATATGG AAGCCGAGAA GAGCCAGGTC 
CACACAGAGT TGCAGGCTCT GCAGAGACAG CTCTCCCAGA ATGAGGAAGA GAAATCCAAG 
TGGGAAGGCA AACAGAACTC CCTAGAATCT GAGCTGATGG AACTGCGTGA AACTGTGGCA 
TCCTTACAGA GTCGCCTCCA GCAAGCAGAG CTGCAAGGAG TGGAAGCCCA GAATGAGCGC 
AAGTTACTGC AGGCAGCCAA GGAGAGCCTG ACTGCCCAGG TGGAACATCT CCAAGCCTCT 
ATCACAGAAG CCAGGGCTCA GGCAAGTGCT GCTGTGGCCC TGGAGGAAGA TCTGCGGATG 
GCGCGCTCAG CCCTGAAACT GAAAAATGAG GAGGTGGCGA GTGAGCGCCA GCGAGCCCAG 
GCCCTGCAGG AGCAGGGTGA GCTGAAAGTG GCCCAGGGGA AGGCTCTGCA GGAGAATCTG 
GCTCGCCTGG CCCAGGCCCT CTCGGAAAGA GAAGGGGAGG TGGAGACTCT GCAGGGGAAA 
ATTGAGGAAC TGGAGAAGCA ACGGGAAATG CAGAAGGCTG TGTTGGAAGT GCTGTCTCTG 
GACCTGAAGA AGAGGAACCG AGAGGTGGAT CTACAGCAGG GACAGATCCA GGAGCTGGAG 
GAGTGTAGGT CTGTGCTAGA GCATCTCCCC GTGGCTGTTC AGGAGCGAGA GCAGCAGCTG 
GCCCTGCAGA GAGAGCGGAT CCGGGAACTT GAGAAGGACC GGGAGACGCA GACGAGCATC 
CTAGAGCATC ATCTCCTGGA ACTTGAGAAG AAAGTCCAAG TGATCGAGTC CCAGAAAGGC 
CAGATTCAGG ACCTGAAGAA GCAGCTGGTT ACGACGGAAT GCCTGGCCCT GGAGCTCGAA 
GAGAAGCGTC ACAAAACGGA GAGCCAGCAA AAGGCGATAG AGGAGCTGGA GGGCCAGAGG 
GAAGCACAGA GGGTGGCTCT GACCCACCTA ACGCTGGACC TGGAGGAAAG GAGCCAGGAA 
CTGCTGGCAC AGGGCAACCG GATCCAGGAG CTGGAGGGCC ACAGCACTCT CCTGGCTAGT 
GAGCTTCAGG AGAAGGAGCA GGAGGCCATG GCCCAGCAAG ACCAGATCCG GGATCTGCAG 
AGGCAGAAAG AGCGTCTAAG TCAAGACCTG GAGAGGAGGG AGCAGGAGCT AGTGCTGCTG 
AAGGAGAGGA CGCAGTCCCT GGAAGATCAG AGGAGCCGGC AGACCAAGAT CCTGGAGGAA 
GACCTGGAGC AGATCAAGCT GGCCCTGAGA GAGCGGGGCC GGGAGCTGGT CTCGCAGCGG 
CAGCTGAGGC AGGAGTGGGC AGAGGAAGGG AAGGGCCAGA GCAAAGCTCA GCGTGGAAGC 
CTGGAGCACA TGAAGCTGAT CCTGCGAGAT AAGGAGAAGG AGGTGGAGTG CCAGCAGGAG 
CACATCCAGG AGCTTCGGGA ACACAGGGAC CAGCTGGAAC AGGAGCTCCA GGGCCTTCAC 
AGGAAGGTGA GTGAGACCAG CCTCCTTTTG ACCCAGCGGG AGCAGGAGAT TGCGGTCCTG 
CAGCAGCACC TGCAGGAAGC CCAGGACCAG GGGGAGCTGA AAGAGCAGGA GCTTCAGGGG 
CAGCTGGAAG AGACCCAGAG GGTCCTGGTG CAGAGGACCC AGGAGCTCCA GGCCCTGCAG 
CAGCAAGAGG CCCAGGACCA GGAGAAGAAC GTGAAGGAGC AGGCAGACTC ACTGCAGACC 
GCCCTGGAGC AGGCGCATGC TGCCCTGGAG GAGCGCCGGG CTGAGCTCGA GGAACACAAG 
GAACACGCGC GGAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCGGCGGAG CCAGGCCCTG 
GAAGAGGTGC TGGGTGACCT TCGGGCAGAG GCTCGGGAGC AGGAGAAGGT GCTGCGGGCC 
CTCCAGCAGC AGTGTGCCGA TCAGGCCCAG GAGCATGAGG CGGAGGCCCG GGGCCTGCGG 
GACAGCTGGC TGCAGGCAGA GGCGCTGCTC GACGCCCGTG ACAGGGAGCT GGAGGCCCTA 
CGGGCAGAGG GCCAGGCCTC CCAACGGCAG GAGGAGGCTG CCCGGGGCCA GGCTGAAGCT 
CTGCAGAAGG CCCTTGGCCA GGCTCAGGCC GCCCTGCGGG AGAAAGAGCA GCATCTTCAT 
GGGCAGGCCG AACTGAGCCG CAACCTGGAG GCCAGCACGG CCACCTTGCA GGCCGCCCTG 
GACTCCTGCC AGGCCCAAGC CCGGCAGCTC GAGGAGACCC TGAGGAAGCG GGAAGGTGAG 
ATCCAAGACG GGGACCTCCG GCACCAGGAG ACCGTGCAGC AGCTCCAGCA GGCGCTTGCC 
CAGAGGGACA AAGAGCTGAG AGAGCTGAGG AGGCAGGGGC AGCTGCTAGA GAAGTCTCTG 
GCCCAAGAGA AGCAGGAGCG CAGGCACGAG AGAGAAGGGG AAGAGGTGAG GGGCCTCCGG 
GAGAGCCTCA GGGAGCTACA GCGGACCCTG GCTCAAAAGG AAGACGAGCT CTTGGAGCTG 
CAGGAGGCCC AGCAAAGGAA GGATCTGGAG GACGCACCCC ACAGCCACAA AACCTCGCCC 
AGGGAGGAGC CGTCTTCCCC ACTCGACTCC TCAGGGCCCA GGCTGCAGCG GGAGCTGGAG 
CGACTGCAGA GGGCTCTGAG GCAGACAGAG GCCAGGGAGA TCGAGTGGAG GGAGAAGGCC 
CAGCACCTGG CGCTGTCCCT GGCCCAGAGC AAGGCCAGCG TGAGCAGTCT GCAGGAGGCG 
GCCCTGCTCC TACAGGCCTC CGTGCTGGAG CGGGACTCGG AGCAGCAGAG GCTGCAGGAC 
GAACTAGAGC TCACCAGACA AGCTCTGGAG AAGGAGCGAC TCCACAGCCT GGGCCTGACC 
AGCCAAGCAG AACAGGGGCC TGGAGGAGAG CCCGGCATCC GGTTGGGAGA GGTCTCAGAG 
GTGAAGATGG AGCCGAGTCC TGAGGCAGAG GAGAAGCAGC GCTGGGAGCA GAGGCTTGAA 
CACCTGCAGC GAGCGGTGGC GCAGCTGGAG GTTGACCGGA GCCGGCTGCA GCACCACAAC 
ACCCAGCTGC GGACCACCCT GGAGCAGGTG GAGCGAGAGC GGAGGAAACT GAAGAGGGAG 
TCCATGCGCG CGTCGAGGGC AGGGGTCCTA GAGAGCAGTG GAGCCACAAC ACCGTCCCCC 
ACACCCCAGG ATGGAAGAGG AGGACAGAAA GTCTCCTCGG ATGCCACGCA CATGGCTGAG 
CTGCAGAAAG AGGTGGCCCT CCTGCGAGCC CAGCTGGCCT TGGAGCGGAA GCAGAAGCAG 
GACTACATCG CCCGCTCGGT GCAGACGAGC CGTGAGCTGG CGGGCCTGCA CCACAGCCTC 
TCTCACTCCC TCCTGGCCGT GGCCCAGGCC CCCGAGGCCA CCGTCCTGGA GGCCGAGACC 
CGCAAGCTGG ATGAGTCCCT GACTCAAAGC CTGACATCGC CCGGGCCCAT CCTGCTGTGC 
CGCAGCCCCA GCCCTGCTCA AGGCGCCTCC AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015466171.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015321657.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

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CloneID	OBa92414
Clone ID Related Accession (Same CDS sequence)	XM_015466171.1 , XM_015474287.1
Accession Version	XM_015466171.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646145.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAT GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCCAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTGA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCAG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015321657.1
CDS	141..5543
Translation	MEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015466171.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015466171.1

ORF Insert Sequence:

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAT GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCCAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTGA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCAG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_015474287.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015329773.1 Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

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CloneID	OBa92414
Clone ID Related Accession (Same CDS sequence)	XM_015466171.1 , XM_015474287.1
Accession Version	XM_015474287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAT GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCCAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTGA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCAG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015329773.1
CDS	141..5543
Translation	MEAAEQARSTLQVDLAEAERSREALWEKNSHLEAQLQKTEETRAELQADLRGIQEEKEEIQEKLSETYHQQEAASAQIEQLKQETTRQEEMLAREVQEKEALAREKAALEVRLQAVERDRQELAEQLLGLSSAKEVLESSLFEAQQQNSLTEVTKGQLEVQIQTVTQAKEVIQGEVKCLQLELDTERSRAEQARDTAARQLAQAEQEGQAALQQLKSAHEEEVNRLQEKWEEERSRHRQELEKVLESLEREKMELEARLREQQAEMEAMRAQREEERAEAESALCQMQLETEKERMSLLETLLQTQKELAEASQQLERLRQDMKVQQLKEQETTGMLQTQLQEAQQALEQAAQQHRDSLATLQQEGRVLLQDKTDLQKQVEDLKSQLLSKEASQRFVDQEVQEKLREAQECSRIQKELEREKASLTLSLVEKEQRLLVLQEADSVQQQELSSLRQDMQEAQAGQKELSTQVELLKQEVKEKEADFLAQETQLLEELEASRVTEQQLRASLWAQETKVAQLQLRLRSTESRLEALVAEQQPGHQAQVQLASLYSVLQQALGSACESRLELNGGGDSAPSLWGPEPDQNGARILFKRGPLLTALSVEAVAAALHKLHQDLWKTQQARDDLRDQAQKLEQRLSDMEAEKSQVHTELQALQRQLSQNEEEKSKWEGKQNSLESELMELRETVASLQSRLQQAELQGVEAQNERKLLQAAKESLTAQVEHLQASITEARAQASAAVALEEDLRMARSALKLKNEEVASERQRAQALQEQGELKVAQGKALQENLARLAQALSEREGEVETLQGKIEELEKQREMQKAVLEVLSLDLKKRNREVDLQQGQIQELEECRSVLEHLPVAVQEREQQLALQRERIRELEKDRETQTSILEHHLLELEKKVQVIESQKGQIQDLKKQLVTTECLALELEEKRHKTESQQKAIEELEGQREAQRVALTHLTLDLEERSQELLAQGNRIQELEGHSTLLASELQEKEQEAMAQQDQIRDLQRQKERLSQDLERREQELVLLKERTQSLEDQRSRQTKILEEDLEQIKLALRERGRELVSQRQLRQEWAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELREHRDQLEQELQGLHRKVSETSLLLTQREQEIAVLQQHLQEAQDQGELKEQELQGQLEETQRVLVQRTQELQALQQQEAQDQEKNVKEQADSLQTALEQAHAALEERRAELEEHKEHARRLQEELAVEGRRSQALEEVLGDLRAEAREQEKVLRALQQQCADQAQEHEAEARGLRDSWLQAEALLDARDRELEALRAEGQASQRQEEAARGQAEALQKALGQAQAALREKEQHLHGQAELSRNLEASTATLQAALDSCQAQARQLEETLRKREGEIQDGDLRHQETVQQLQQALAQRDKELRELRRQGQLLEKSLAQEKQERRHEREGEEVRGLRESLRELQRTLAQKEDELLELQEAQQRKDLEDAPHSHKTSPREEPSSPLDSSGPRLQRELERLQRALRQTEAREIEWREKAQHLALSLAQSKASVSSLQEAALLLQASVLERDSEQQRLQDELELTRQALEKERLHSLGLTSQAEQGPGGEPGIRLGEVSEVKMEPSPEAEEKQRWEQRLEHLQRAVAQLEVDRSRLQHHNTQLRTTLEQVERERRKLKRESMRASRAGVLESSGATTPSPTPQDGRGGQKVSSDATHMAELQKEVALLRAQLALERKQKQDYIARSVQTSRELAGLHHSLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPILLCRSPSPAQGASR

Target ORF information:

RefSeq Version	XM_015474287.1
Organism	Bos taurus(cattle)
Definition	Bos taurus centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474287.1

ORF Insert Sequence:

ATGGAGGCAG CAGAGCAGGC GAGAAGCACT TTGCAGGTGG ACCTGGCGGA GGCCGAGAGG 
AGCAGGGAAG CCCTGTGGGA AAAGAACAGC CACCTGGAGG CCCAGCTGCA GAAAACAGAG 
GAGACCAGGG CGGAGCTGCA AGCGGACCTC AGGGGCATCC AAGAAGAGAA GGAAGAAATT 
CAAGAGAAGC TAAGCGAGAC ATATCACCAG CAGGAGGCAG CTTCAGCTCA GATAGAACAG 
CTAAAGCAAG AGACAACGCG CCAGGAAGAG ATGCTCGCCC GAGAAGTCCA GGAGAAGGAG 
GCTCTGGCAC GGGAGAAGGC AGCTCTCGAG GTGCGGCTAC AGGCTGTGGA GCGAGACCGG 
CAGGAGCTCG CAGAACAACT TCTGGGGCTC AGCTCAGCCA AGGAGGTACT GGAGAGCAGT 
CTGTTTGAGG CCCAGCAACA AAATTCTCTG ACAGAGGTCA CCAAAGGGCA GCTGGAGGTC 
CAGATTCAAA CTGTCACGCA AGCCAAGGAA GTAATCCAAG GGGAAGTGAA GTGCCTGCAG 
CTGGAACTGG ACACTGAACG GAGCCGGGCA GAGCAGGCAC GGGACACAGC AGCCAGGCAG 
CTGGCCCAGG CTGAGCAAGA AGGGCAGGCT GCCCTGCAGC AGCTGAAGTC GGCCCATGAG 
GAGGAGGTGA ATCGGCTCCA GGAGAAGTGG GAAGAAGAGC GCTCCCGGCA CCGGCAGGAA 
CTGGAGAAGG TTCTGGAGAG CCTGGAGAGG GAGAAGATGG AGCTGGAAGC GAGGCTGAGG 
GAACAACAGG CAGAAATGGA GGCTATGCGG GCGCAGAGGG AGGAAGAGCG GGCCGAGGCC 
GAGAGCGCCC TGTGCCAGAT GCAGCTCGAG ACAGAGAAGG AGAGGATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GAGGCCAGCC AACAGCTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGCA GTTAAAGGAG CAGGAGACCA CCGGGATGCT GCAGACCCAG 
CTCCAGGAGG CTCAGCAGGC ACTGGAGCAG GCAGCCCAGC AGCACAGAGA CAGTCTGGCG 
ACCCTCCAGC AGGAGGGCCG AGTCCTGCTG CAGGATAAGA CAGACCTGCA GAAGCAGGTG 
GAAGACTTGA AGTCTCAGCT GCTTTCCAAA GAAGCCTCCC AGAGGTTCGT GGACCAGGAG 
GTTCAGGAAA AGCTGAGAGA GGCCCAGGAG TGTAGCCGAA TTCAGAAGGA GCTGGAGAGA 
GAAAAAGCCA GCCTGACTCT GTCGCTGGTG GAAAAGGAAC AGAGACTCCT TGTTCTGCAA 
GAGGCTGACT CTGTTCAACA GCAAGAGCTG AGCTCCCTGC GCCAGGATAT GCAGGAGGCC 
CAGGCCGGGC AGAAAGAGCT CAGCACCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGAA 
AAGGAGGCTG ACTTCCTGGC CCAGGAGACT CAGCTGCTGG AGGAGCTTGA GGCGTCTCGA 
GTCACCGAAC AACAGCTCCG AGCTTCCTTG TGGGCCCAGG AGACCAAGGT GGCCCAACTA 
CAGCTGCGCC TGCGCAGCAC AGAGAGCCGG CTGGAGGCGC TGGTGGCGGA GCAGCAGCCT 
GGGCACCAGG CCCAGGTCCA GCTGGCCAGC CTCTATTCTG TCCTGCAGCA GGCCCTGGGG 
TCTGCCTGCG AGAGCAGGCT GGAGCTAAAT GGCGGGGGAG ACTCAGCACC CTCCCTCTGG 
GGCCCGGAGC CAGACCAGAA TGGCGCTAGG ATCCTCTTTA AGAGAGGGCC CCTCCTGACG 
GCTCTCTCAG TTGAGGCAGT GGCGGCCGCC CTCCACAAGC TTCACCAAGA CCTGTGGAAG 
ACGCAGCAGG CCCGGGATGA TCTGCGGGAT CAAGCCCAGA AGCTGGAACA GCGTCTCAGT 
GATATGGAAG CCGAGAAGAG CCAGGTCCAC ACAGAGTTGC AGGCTCTGCA GAGACAGCTC 
TCCCAGAATG AGGAAGAGAA ATCCAAGTGG GAAGGCAAAC AGAACTCCCT AGAATCTGAG 
CTGATGGAAC TGCGTGAAAC TGTGGCATCC TTACAGAGTC GCCTCCAGCA AGCAGAGCTG 
CAAGGAGTGG AAGCCCAGAA TGAGCGCAAG TTACTGCAGG CAGCCAAGGA GAGCCTGACT 
GCCCAGGTGG AACATCTCCA AGCCTCTATC ACAGAAGCCA GGGCTCAGGC AAGTGCTGCT 
GTGGCCCTGG AGGAAGATCT GCGGATGGCG CGCTCAGCCC TGAAACTGAA AAATGAGGAG 
GTGGCGAGTG AGCGCCAGCG AGCCCAGGCC CTGCAGGAGC AGGGTGAGCT GAAAGTGGCC 
CAGGGGAAGG CTCTGCAGGA GAATCTGGCT CGCCTGGCCC AGGCCCTCTC GGAAAGAGAA 
GGGGAGGTGG AGACTCTGCA GGGGAAAATT GAGGAACTGG AGAAGCAACG GGAAATGCAG 
AAGGCTGTGT TGGAAGTGCT GTCTCTGGAC CTGAAGAAGA GGAACCGAGA GGTGGATCTA 
CAGCAGGGAC AGATCCAGGA GCTGGAGGAG TGTAGGTCTG TGCTAGAGCA TCTCCCCGTG 
GCTGTTCAGG AGCGAGAGCA GCAGCTGGCC CTGCAGAGAG AGCGGATCCG GGAACTTGAG 
AAGGACCGGG AGACGCAGAC GAGCATCCTA GAGCATCATC TCCTGGAACT TGAGAAGAAA 
GTCCAAGTGA TCGAGTCCCA GAAAGGCCAG ATTCAGGACC TGAAGAAGCA GCTGGTTACG 
ACGGAATGCC TGGCCCTGGA GCTCGAAGAG AAGCGTCACA AAACGGAGAG CCAGCAAAAG 
GCGATAGAGG AGCTGGAGGG CCAGAGGGAA GCACAGAGGG TGGCTCTGAC CCACCTAACG 
CTGGACCTGG AGGAAAGGAG CCAGGAACTG CTGGCACAGG GCAACCGGAT CCAGGAGCTG 
GAGGGCCACA GCACTCTCCT GGCTAGTGAG CTTCAGGAGA AGGAGCAGGA GGCCATGGCC 
CAGCAAGACC AGATCCGGGA TCTGCAGAGG CAGAAAGAGC GTCTAAGTCA AGACCTGGAG 
AGGAGGGAGC AGGAGCTAGT GCTGCTGAAG GAGAGGACGC AGTCCCTGGA AGATCAGAGG 
AGCCGGCAGA CCAAGATCCT GGAGGAAGAC CTGGAGCAGA TCAAGCTGGC CCTGAGAGAG 
CGGGGCCGGG AGCTGGTCTC GCAGCGGCAG CTGAGGCAGG AGTGGGCAGA GGAAGGGAAG 
GGCCAGAGCA AAGCTCAGCG TGGAAGCCTG GAGCACATGA AGCTGATCCT GCGAGATAAG 
GAGAAGGAGG TGGAGTGCCA GCAGGAGCAC ATCCAGGAGC TTCGGGAACA CAGGGACCAG 
CTGGAACAGG AGCTCCAGGG CCTTCACAGG AAGGTGAGTG AGACCAGCCT CCTTTTGACC 
CAGCGGGAGC AGGAGATTGC GGTCCTGCAG CAGCACCTGC AGGAAGCCCA GGACCAGGGG 
GAGCTGAAAG AGCAGGAGCT TCAGGGGCAG CTGGAAGAGA CCCAGAGGGT CCTGGTGCAG 
AGGACCCAGG AGCTCCAGGC CCTGCAGCAG CAAGAGGCCC AGGACCAGGA GAAGAACGTG 
AAGGAGCAGG CAGACTCACT GCAGACCGCC CTGGAGCAGG CGCATGCTGC CCTGGAGGAG 
CGCCGGGCTG AGCTCGAGGA ACACAAGGAA CACGCGCGGA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC GGCGGAGCCA GGCCCTGGAA GAGGTGCTGG GTGACCTTCG GGCAGAGGCT 
CGGGAGCAGG AGAAGGTGCT GCGGGCCCTC CAGCAGCAGT GTGCCGATCA GGCCCAGGAG 
CATGAGGCGG AGGCCCGGGG CCTGCGGGAC AGCTGGCTGC AGGCAGAGGC GCTGCTCGAC 
GCCCGTGACA GGGAGCTGGA GGCCCTACGG GCAGAGGGCC AGGCCTCCCA ACGGCAGGAG 
GAGGCTGCCC GGGGCCAGGC TGAAGCTCTG CAGAAGGCCC TTGGCCAGGC TCAGGCCGCC 
CTGCGGGAGA AAGAGCAGCA TCTTCATGGG CAGGCCGAAC TGAGCCGCAA CCTGGAGGCC 
AGCACGGCCA CCTTGCAGGC CGCCCTGGAC TCCTGCCAGG CCCAAGCCCG GCAGCTCGAG 
GAGACCCTGA GGAAGCGGGA AGGTGAGATC CAAGACGGGG ACCTCCGGCA CCAGGAGACC 
GTGCAGCAGC TCCAGCAGGC GCTTGCCCAG AGGGACAAAG AGCTGAGAGA GCTGAGGAGG 
CAGGGGCAGC TGCTAGAGAA GTCTCTGGCC CAAGAGAAGC AGGAGCGCAG GCACGAGAGA 
GAAGGGGAAG AGGTGAGGGG CCTCCGGGAG AGCCTCAGGG AGCTACAGCG GACCCTGGCT 
CAAAAGGAAG ACGAGCTCTT GGAGCTGCAG GAGGCCCAGC AAAGGAAGGA TCTGGAGGAC 
GCACCCCACA GCCACAAAAC CTCGCCCAGG GAGGAGCCGT CTTCCCCACT CGACTCCTCA 
GGGCCCAGGC TGCAGCGGGA GCTGGAGCGA CTGCAGAGGG CTCTGAGGCA GACAGAGGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG CACCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGCGTGA GCAGTCTGCA GGAGGCGGCC CTGCTCCTAC AGGCCTCCGT GCTGGAGCGG 
GACTCGGAGC AGCAGAGGCT GCAGGACGAA CTAGAGCTCA CCAGACAAGC TCTGGAGAAG 
GAGCGACTCC ACAGCCTGGG CCTGACCAGC CAAGCAGAAC AGGGGCCTGG AGGAGAGCCC 
GGCATCCGGT TGGGAGAGGT CTCAGAGGTG AAGATGGAGC CGAGTCCTGA GGCAGAGGAG 
AAGCAGCGCT GGGAGCAGAG GCTTGAACAC CTGCAGCGAG CGGTGGCGCA GCTGGAGGTT 
GACCGGAGCC GGCTGCAGCA CCACAACACC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGAGTCC ATGCGCGCGT CGAGGGCAGG GGTCCTAGAG 
AGCAGTGGAG CCACAACACC GTCCCCCACA CCCCAGGATG GAAGAGGAGG ACAGAAAGTC 
TCCTCGGATG CCACGCACAT GGCTGAGCTG CAGAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATCGCCC GCTCGGTGCA GACGAGCCGT 
GAGCTGGCGG GCCTGCACCA CAGCCTCTCT CACTCCCTCC TGGCCGTGGC CCAGGCCCCC 
GAGGCCACCG TCCTGGAGGC CGAGACCCGC AAGCTGGATG AGTCCCTGAC TCAAAGCCTG 
ACATCGCCCG GGCCCATCCT GCTGTGCCGC AGCCCCAGCC CTGCTCAAGG CGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CEP250
Entrez Gene ID	507479
Full Name	centrosomal protein 250
General protein information	Preferred Names centrosomal protein 250 Names centrosome-associated protein CEP250
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Gallus gallus (chicken)	LOC100858952	XP_003642497.2
Homo sapiens (human)	CEP250	NP_009117.2
Pan troglodytes (chimpanzee)	CEP250	XP_003316929.1
Mus musculus (house mouse)	Cep250	NP_001123471.1
Bos taurus (cattle)	CEP250	XP_005215062.1
Gallus gallus (chicken)	CEP250	XP_417323.4
	CEP250	XP_001099508.1
Canis lupus familiaris (dog)	CEP250	XP_542975.3
Rattus norvegicus (Norway rat)	Cep250	XP_006235436.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CEP250 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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