GRAMD1A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005219085.3	XP_005219142.1	GRAM domain-containing protein 1A isoform X2
XM_010814852.2	XP_010813154.1	GRAM domain-containing protein 1A isoform X4
NM_001305021.1	NP_001291950.1	GRAM domain-containing protein 1A
XM_024978041.1	XP_024833809.1	GRAM domain-containing protein 1A isoform X1
XM_005219085.4	XP_005219142.2	GRAM domain-containing protein 1A isoform X2
XM_010814851.3	XP_010813153.2	GRAM domain-containing protein 1A isoform X5
XM_005219087.3	XP_005219144.1	GRAM domain-containing protein 1A isoform X3
NM_001305021.2	NP_001291950.2	GRAM domain-containing protein 1A
XM_010814850.3	XP_010813152.2	GRAM domain-containing protein 1A isoform X5
XM_005219084.3	XP_005219141.1	GRAM domain-containing protein 1A isoform X1
XM_010814851.2	XP_010813153.1	GRAM domain-containing protein 1A isoform X4
XM_010814850.2	XP_010813152.1	GRAM domain-containing protein 1A isoform X4
XM_005219087.4	XP_005219144.2	GRAM domain-containing protein 1A isoform X4
XM_024978042.1	XP_024833810.1	GRAM domain-containing protein 1A isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa28731	NM_001305021.1 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa28729	XM_005219084.3 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa28730	XM_005219085.3 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa28732	XM_005219087.3 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46732	XM_010814851.2 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46732	XM_010814852.2 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46732	XM_010814850.2 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa239039	NM_001305021.2 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224857	XM_024978041.1 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224858	XM_005219085.4 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224859	XM_024978042.1 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224860	XM_005219087.4 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224861	XM_010814850.3 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224861	XM_010814851.3 Latest version!	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

GRAMD1A ( NM_001305021.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001291950.1 Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.

Price & Availability↑

CloneID	OBa28731
Clone ID Related Accession (Same CDS sequence)	NM_001305021.1
Accession Version	NM_001305021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02046930.1 and DAAA02046931.1. On or before Feb 14, 2015 this sequence version replaced XM_005219086.2, XM_005192729.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02947219, SAMN02947220 [ECO:0000348] ##Evidence-Data-END##

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACGTACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCAG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA CGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291950.1
CDS	61..2235
Translation	MFDTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	NM_001305021.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305021.1

ORF Insert Sequence:

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACGTACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCAG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA CGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005219084.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219141.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa28729
Clone ID Related Accession (Same CDS sequence)	XM_005219084.3
Accession Version	XM_005219084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219084.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACGT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCAGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTGATCT GTGTGAGCCT CATCGTCCTC ATTGCCCTCA ACGTCCTCCT CTTTTACCGC 
CTCTGGTCCC TGGAGAGGAC AGCTCACACT TTTGAGTCCT GGCACAGTCT GGCCCTGGCC 
AAGGGTAAGT TCCCCCAGAC GGCCACGGAG TGGGCGGAGA TCCTGGCCCT GCAGAAGCAG 
TTCCACAGCG TCGACGTGCA CAAGTGGAGG CAGATCCTGC GGGCTTCTGT GGAGCTCCTG 
GACGAGATGA AGTTCTCACT GGAGAAGCTA CATCAAGGCA TCACCGTCTC AGACCCTCCC 
TTCGACTCCC AGCCACAGCC TGACGACAGC TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219141.1
CDS	2646..5084
Translation	MAEFLPLLPCTPLLTQDVCAQDSRVPPAKEPSQEAQGGRVHGPAPKRWKLLEVPTIQITPASNGESPPCTPPAQRLQLPRTPDRESSPQDSTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_005219084.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219084.3

ORF Insert Sequence:

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACGT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCAGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTGATCT GTGTGAGCCT CATCGTCCTC ATTGCCCTCA ACGTCCTCCT CTTTTACCGC 
CTCTGGTCCC TGGAGAGGAC AGCTCACACT TTTGAGTCCT GGCACAGTCT GGCCCTGGCC 
AAGGGTAAGT TCCCCCAGAC GGCCACGGAG TGGGCGGAGA TCCTGGCCCT GCAGAAGCAG 
TTCCACAGCG TCGACGTGCA CAAGTGGAGG CAGATCCTGC GGGCTTCTGT GGAGCTCCTG 
GACGAGATGA AGTTCTCACT GGAGAAGCTA CATCAAGGCA TCACCGTCTC AGACCCTCCC 
TTCGACTCCC AGCCACAGCC TGACGACAGC TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005219085.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219142.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa28730
Clone ID Related Accession (Same CDS sequence)	XM_005219085.3
Accession Version	XM_005219085.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219085.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACGT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCAGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTCCTCA TCGTCCTCAT TGCCCTCAAC GTCCTCCTCT TTTACCGCCT CTGGTCCCTG 
GAGAGGACAG CTCACACTTT TGAGTCCTGG CACAGTCTGG CCCTGGCCAA GGGTAAGTTC 
CCCCAGACGG CCACGGAGTG GGCGGAGATC CTGGCCCTGC AGAAGCAGTT CCACAGCGTC 
GACGTGCACA AGTGGAGGCA GATCCTGCGG GCTTCTGTGG AGCTCCTGGA CGAGATGAAG 
TTCTCACTGG AGAAGCTACA TCAAGGCATC ACCGTCTCAG ACCCTCCCTT CGACTCCCAG 
CCACAGCCTG ACGACAGCTT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219142.1
CDS	174..2600
Translation	MAEFLPLLPCTPLLTQDVCAQDSRVPPAKEPSQEAQGGRVHGPAPKRWKLLEVPTIQITPASNGESPPCTPPAQRLQLPRTPDRESSPQDSTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_005219085.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219085.3

ORF Insert Sequence:

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACGT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCAGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTCCTCA TCGTCCTCAT TGCCCTCAAC GTCCTCCTCT TTTACCGCCT CTGGTCCCTG 
GAGAGGACAG CTCACACTTT TGAGTCCTGG CACAGTCTGG CCCTGGCCAA GGGTAAGTTC 
CCCCAGACGG CCACGGAGTG GGCGGAGATC CTGGCCCTGC AGAAGCAGTT CCACAGCGTC 
GACGTGCACA AGTGGAGGCA GATCCTGCGG GCTTCTGTGG AGCTCCTGGA CGAGATGAAG 
TTCTCACTGG AGAAGCTACA TCAAGGCATC ACCGTCTCAG ACCCTCCCTT CGACTCCCAG 
CCACAGCCTG ACGACAGCTT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005219087.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219144.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa28732
Clone ID Related Accession (Same CDS sequence)	XM_005219087.3
Accession Version	XM_005219087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACGTACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCAG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TCCTCATCGT CCTCATTGCC 
CTCAACGTCC TCCTCTTTTA CCGCCTCTGG TCCCTGGAGA GGACAGCTCA CACTTTTGAG 
TCCTGGCACA GTCTGGCCCT GGCCAAGGGT AAGTTCCCCC AGACGGCCAC GGAGTGGGCG 
GAGATCCTGG CCCTGCAGAA GCAGTTCCAC AGCGTCGACG TGCACAAGTG GAGGCAGATC 
CTGCGGGCTT CTGTGGAGCT CCTGGACGAG ATGAAGTTCT CACTGGAGAA GCTACATCAA 
GGCATCACCG TCTCAGACCC TCCCTTCGAC TCCCAGCCAC AGCCTGACGA CAGCTTCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219144.1
CDS	88..2250
Translation	MFDTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_005219087.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219087.3

ORF Insert Sequence:

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACGTACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCAG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TCCTCATCGT CCTCATTGCC 
CTCAACGTCC TCCTCTTTTA CCGCCTCTGG TCCCTGGAGA GGACAGCTCA CACTTTTGAG 
TCCTGGCACA GTCTGGCCCT GGCCAAGGGT AAGTTCCCCC AGACGGCCAC GGAGTGGGCG 
GAGATCCTGG CCCTGCAGAA GCAGTTCCAC AGCGTCGACG TGCACAAGTG GAGGCAGATC 
CTGCGGGCTT CTGTGGAGCT CCTGGACGAG ATGAAGTTCT CACTGGAGAA GCTACATCAA 
GGCATCACCG TCTCAGACCC TCCCTTCGAC TCCCAGCCAC AGCCTGACGA CAGCTTCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_010814852.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813154.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa46732
Clone ID Related Accession (Same CDS sequence)	XM_010814852.2 , XM_010814851.2 , XM_010814850.2
Accession Version	XM_010814852.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813154.1
CDS	237..2294
Translation	MVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_010814852.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814852.2

ORF Insert Sequence:

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_010814851.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813153.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa46732
Clone ID Related Accession (Same CDS sequence)	XM_010814852.2 , XM_010814851.2 , XM_010814850.2
Accession Version	XM_010814851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813153.1
CDS	354..2411
Translation	MVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_010814851.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814851.2

ORF Insert Sequence:

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_010814850.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813152.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa46732
Clone ID Related Accession (Same CDS sequence)	XM_010814852.2 , XM_010814851.2 , XM_010814850.2
Accession Version	XM_010814850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813152.1
CDS	265..2322
Translation	MVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVDVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_010814850.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814850.2

ORF Insert Sequence:

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACGTA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CAGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGACGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( NM_001305021.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001291950.2 Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.

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CloneID	OBa239039
Clone ID Related Accession (Same CDS sequence)	NM_001305021.2
Accession Version	NM_001305021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000018.1. On May 30, 2018 this sequence version replaced NM_001305021.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571263.29002.1, SRR5571256.1628.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA GGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291950.2
CDS	61..2235
Translation	MFDTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	NM_001305021.2
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305021.2

ORF Insert Sequence:

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA GGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_024978041.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833809.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

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CloneID	OBa224857
Clone ID Related Accession (Same CDS sequence)	XM_024978041.1
Accession Version	XM_024978041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACAT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCGGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTGATCT GTGTGAGCCT CATCGTCCTC ATTGCCCTCA ACGTCCTCCT CTTTTACCGC 
CTCTGGTCCC TGGAGAGGAC AGCTCACACT TTTGAGTCCT GGCACAGTCT GGCCCTGGCC 
AAGGGTAAGT TCCCCCAGAC GGCCACGGAG TGGGCGGAGA TCCTGGCCCT GCAGAAGCAG 
TTCCACAGCG TCGAGGTGCA CAAGTGGAGG CAGATCCTGC GGGCTTCTGT GGAGCTCCTG 
GACGAGATGA AGTTCTCACT GGAGAAGCTA CATCAAGGCA TCACCGTCTC AGACCCTCCC 
TTCGACTCCC AGCCACAGCC TGACGACAGC TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833809.1
CDS	32..2470
Translation	MAEFLPLLPCTPLLTQDVCAQDSRVPPAKEPSQEAQGGRVHGPAPKRWKLLEVPTIQITPASNGESPPCTPPAQRLQLPRTPDRESSPQDSTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_024978041.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978041.1

ORF Insert Sequence:

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACAT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCGGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTGATCT GTGTGAGCCT CATCGTCCTC ATTGCCCTCA ACGTCCTCCT CTTTTACCGC 
CTCTGGTCCC TGGAGAGGAC AGCTCACACT TTTGAGTCCT GGCACAGTCT GGCCCTGGCC 
AAGGGTAAGT TCCCCCAGAC GGCCACGGAG TGGGCGGAGA TCCTGGCCCT GCAGAAGCAG 
TTCCACAGCG TCGAGGTGCA CAAGTGGAGG CAGATCCTGC GGGCTTCTGT GGAGCTCCTG 
GACGAGATGA AGTTCTCACT GGAGAAGCTA CATCAAGGCA TCACCGTCTC AGACCCTCCC 
TTCGACTCCC AGCCACAGCC TGACGACAGC TTCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005219085.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219142.2 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224858
Clone ID Related Accession (Same CDS sequence)	XM_005219085.4
Accession Version	XM_005219085.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219085.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACAT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCGGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTCCTCA TCGTCCTCAT TGCCCTCAAC GTCCTCCTCT TTTACCGCCT CTGGTCCCTG 
GAGAGGACAG CTCACACTTT TGAGTCCTGG CACAGTCTGG CCCTGGCCAA GGGTAAGTTC 
CCCCAGACGG CCACGGAGTG GGCGGAGATC CTGGCCCTGC AGAAGCAGTT CCACAGCGTC 
GAGGTGCACA AGTGGAGGCA GATCCTGCGG GCTTCTGTGG AGCTCCTGGA CGAGATGAAG 
TTCTCACTGG AGAAGCTACA TCAAGGCATC ACCGTCTCAG ACCCTCCCTT CGACTCCCAG 
CCACAGCCTG ACGACAGCTT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219142.2
CDS	30..2456
Translation	MAEFLPLLPCTPLLTQDVCAQDSRVPPAKEPSQEAQGGRVHGPAPKRWKLLEVPTIQITPASNGESPPCTPPAQRLQLPRTPDRESSPQDSTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_005219085.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219085.4

ORF Insert Sequence:

ATGGCTGAGT TTCTGCCCCT GCTGCCTTGT ACACCCTTGC TGACCCAGGA TGTCTGTGCC 
CAGGACTCAC GGGTGCCCCC AGCCAAGGAG CCGAGCCAGG AGGCACAGGG TGGCAGGGTC 
CATGGCCCGG CCCCTAAGCG CTGGAAGCTC CTGGAGGTGC CCACCATTCA GATAACACCA 
GCCAGCAATG GGGAGTCACC CCCTTGCACT CCACCCGCCC AGCGCCTGCA GCTGCCGAGG 
ACCCCAGACC GGGAGAGTTC GCCCCAGGAC AGCACAACCC CCCACTCTGG CCGGAGCACG 
CCAAGCAGCT CTCCATCCCT CCGTAAGCGC CTGCAGCTCT TGCCCACAAG CCGACCCCCG 
CCTGAGCCAG AACCGGGCAC CATGGTGGAG AAGGGGTCGG ATAGCTCCTC AGAGAAGGGT 
GGGGTGCCTG GGACACCCAG CACCCAGAGC CTGGGCAGCC GGAACTTCAT CCGCAACAGC 
AAGAAGATGC AGAGCTGGTA CAGTATGCTG AGCCCCACAT ACAAACAGCG GAACGAGGAC 
TTCCGGAAAC TGTTCAGCAA ACTCCCTGAA GCAGAACGCC TCATTGTGGA TTACTCCTGT 
GCCTTGCAGC GCGAGATCCT CCTCCAGGGC CGCCTCTATC TCTCCGAGAA CTGGATCTGC 
TTCTACAGCA ACATCTTCCG CTGGGAGACA ACCATCTCCA TCCAGTTGAA GGAAGTGACG 
TGTCTGAAGA AGGAAAAGAC GGCCAAGCTG ATCCCCAATG CCATCCAGAT CTGCACGGAG 
AACGAGAAGC ATTTCTTCAC CTCCTTTGGG GCCCGAGATC GCTGCTTCCT CCTCATCTTC 
CGCCTCTGGC AGAACGCGCT GCTTGAAAAG ACGCTGAGTC CCCGCGAGCT CTGGCACCTG 
GTGCATCAGT GCTACGGCTC AGAGCTGGGC CTCACCAGCG AGGACGAGGA CTATGTCTGC 
CCCTTACAGC TGAATGGCCT AGGGAGCCCC AAGGATGTGG GCGATGTAAT TGCCCTGAGC 
GACATCACCC CCTCGGGGGC AGCTGACCAC AGCCAGGAGC CAAGCCCCGC AGGTTCACGC 
CGTGGCCGCA TCACCCCCAA CCTGTCCCGG GCCAGCAGTG ATGCAGACCA TGGGGCAGAG 
GAGGACAAGG AGGAGCAGAC AGACAGCCAG CCAGATGCCT CCTCCAGCCA GACAGTGACC 
CCGGTGGCTG AACCCCCGAG CGCAGAGCCC ACCCCACCTG ACGGGCCCAC CTCCCTGGGT 
CCCTTGGATC TGCTGCCCAG TGAGGAGCTG TTGACAGACA CAAGTAACTC CTCCTCGTCC 
ACGGGGGAGG AAGCCGACCT GGCGGCCCTG CTGCCTGACC TCTCCGGCCG TCTCCTCATC 
AACTCCGTCT TCCACGTGGG TGCTGAGCGG CTCCAGCAGA TGCTCTTCTC AGACTCGCCC 
TTCCTGCAGG ACTTCCTGCA GCAGTGCAAG TTCACAGACG TGACCTTGAG CCCCTGGAGT 
GGGGACAGCA AATGCCACCA GCGCCGAGTG CTGACCTACA CCATTCCCAT CAGCAACCCG 
CTGGGGCCCA AGAGCGCCTC TGTGGTGGAG ACACAGACGC TGTTCCGGCG TGGCCCCCAG 
GCGGGAGGGT GTGTGGTAGA CTCCGAGGTG CTGACGCAGG GCATCCCTTA CCAAGACTAC 
TTCTACACTG CCCACCGCTA CTGCATCCTG GGCCTCGCCC GGAACAAGGC CCGCCTCCGG 
GTGTCCTCCG AGATCCGCTA CCGGAAGCAG CCCTGGAGCC TCGTGAAGTC CCTGATTGAG 
AAGAACTCAT GGAGTGGCAT CGAAGATTAC TTCCACCACC TGGAGCGAGA ACTCGCCAAG 
GCCGAGAAGC TGTCCCTGGA GGAAGGCGGG AAGGACACGC GGGGCTTGCT GTCTGGCCTG 
CGCCGGCGGA AGCGGCCTCT GAGCTGGAGG GCTCACGGTG ATGGGACCCC ACACCCGGAC 
CCTGACCCCT GTGCCCGGAC TGGCATGCAC ACCTCGGGCT CCCTCAGCTC CCGCTTCTCG 
GAACCGTCTA TGGACCAGGG CCCCGGGGCA GGCACCCCCA GCGCCCTGGT TCTCATCAGC 
ATTGTCCTCA TCGTCCTCAT TGCCCTCAAC GTCCTCCTCT TTTACCGCCT CTGGTCCCTG 
GAGAGGACAG CTCACACTTT TGAGTCCTGG CACAGTCTGG CCCTGGCCAA GGGTAAGTTC 
CCCCAGACGG CCACGGAGTG GGCGGAGATC CTGGCCCTGC AGAAGCAGTT CCACAGCGTC 
GAGGTGCACA AGTGGAGGCA GATCCTGCGG GCTTCTGTGG AGCTCCTGGA CGAGATGAAG 
TTCTCACTGG AGAAGCTACA TCAAGGCATC ACCGTCTCAG ACCCTCCCTT CGACTCCCAG 
CCACAGCCTG ACGACAGCTT CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_024978042.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024833810.1 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.

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CloneID	OBa224859
Clone ID Related Accession (Same CDS sequence)	XM_024978042.1
Accession Version	XM_024978042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT TTAACCCTCC AAACAAGAAG ATGACATGTG TTTCTTACCC CTGGGTTATA 
GATGAACTCA CCAAGGACAG GGAGCTGGGG GGCCTGCTCT GGATCATTCT GCGAAGAGGT 
GGAACAGGCA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA GGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024833810.1
CDS	659..2953
Translation	MDLFNPPNKKMTCVSYPWVIDELTKDRELGGLLWIILRRGGTGTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_024978042.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024978042.1

ORF Insert Sequence:

ATGGACTTGT TTAACCCTCC AAACAAGAAG ATGACATGTG TTTCTTACCC CTGGGTTATA 
GATGAACTCA CCAAGGACAG GGAGCTGGGG GGCCTGCTCT GGATCATTCT GCGAAGAGGT 
GGAACAGGCA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TGATCTGTGT GAGCCTCATC 
GTCCTCATTG CCCTCAACGT CCTCCTCTTT TACCGCCTCT GGTCCCTGGA GAGGACAGCT 
CACACTTTTG AGTCCTGGCA CAGTCTGGCC CTGGCCAAGG GTAAGTTCCC CCAGACGGCC 
ACGGAGTGGG CGGAGATCCT GGCCCTGCAG AAGCAGTTCC ACAGCGTCGA GGTGCACAAG 
TGGAGGCAGA TCCTGCGGGC TTCTGTGGAG CTCCTGGACG AGATGAAGTT CTCACTGGAG 
AAGCTACATC AAGGCATCAC CGTCTCAGAC CCTCCCTTCG ACTCCCAGCC ACAGCCTGAC 
GACAGCTTCT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_005219087.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219144.2 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa224860
Clone ID Related Accession (Same CDS sequence)	XM_005219087.4
Accession Version	XM_005219087.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219087.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TCCTCATCGT CCTCATTGCC 
CTCAACGTCC TCCTCTTTTA CCGCCTCTGG TCCCTGGAGA GGACAGCTCA CACTTTTGAG 
TCCTGGCACA GTCTGGCCCT GGCCAAGGGT AAGTTCCCCC AGACGGCCAC GGAGTGGGCG 
GAGATCCTGG CCCTGCAGAA GCAGTTCCAC AGCGTCGAGG TGCACAAGTG GAGGCAGATC 
CTGCGGGCTT CTGTGGAGCT CCTGGACGAG ATGAAGTTCT CACTGGAGAA GCTACATCAA 
GGCATCACCG TCTCAGACCC TCCCTTCGAC TCCCAGCCAC AGCCTGACGA CAGCTTCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219144.2
CDS	855..3017
Translation	MFDTTPHSGRSTPSSSPSLRKRLQLLPTSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_005219087.4
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219087.4

ORF Insert Sequence:

ATGTTCGACA CAACCCCCCA CTCTGGCCGG AGCACGCCAA GCAGCTCTCC ATCCCTCCGT 
AAGCGCCTGC AGCTCTTGCC CACAAGCCGA CCCCCGCCTG AGCCAGAACC GGGCACCATG 
GTGGAGAAGG GGTCGGATAG CTCCTCAGAG AAGGGTGGGG TGCCTGGGAC ACCCAGCACC 
CAGAGCCTGG GCAGCCGGAA CTTCATCCGC AACAGCAAGA AGATGCAGAG CTGGTACAGT 
ATGCTGAGCC CCACATACAA ACAGCGGAAC GAGGACTTCC GGAAACTGTT CAGCAAACTC 
CCTGAAGCAG AACGCCTCAT TGTGGATTAC TCCTGTGCCT TGCAGCGCGA GATCCTCCTC 
CAGGGCCGCC TCTATCTCTC CGAGAACTGG ATCTGCTTCT ACAGCAACAT CTTCCGCTGG 
GAGACAACCA TCTCCATCCA GTTGAAGGAA GTGACGTGTC TGAAGAAGGA AAAGACGGCC 
AAGCTGATCC CCAATGCCAT CCAGATCTGC ACGGAGAACG AGAAGCATTT CTTCACCTCC 
TTTGGGGCCC GAGATCGCTG CTTCCTCCTC ATCTTCCGCC TCTGGCAGAA CGCGCTGCTT 
GAAAAGACGC TGAGTCCCCG CGAGCTCTGG CACCTGGTGC ATCAGTGCTA CGGCTCAGAG 
CTGGGCCTCA CCAGCGAGGA CGAGGACTAT GTCTGCCCCT TACAGCTGAA TGGCCTAGGG 
AGCCCCAAGG ATGTGGGCGA TGTAATTGCC CTGAGCGACA TCACCCCCTC GGGGGCAGCT 
GACCACAGCC AGGAGCCAAG CCCCGCAGGT TCACGCCGTG GCCGCATCAC CCCCAACCTG 
TCCCGGGCCA GCAGTGATGC AGACCATGGG GCAGAGGAGG ACAAGGAGGA GCAGACAGAC 
AGCCAGCCAG ATGCCTCCTC CAGCCAGACA GTGACCCCGG TGGCTGAACC CCCGAGCGCA 
GAGCCCACCC CACCTGACGG GCCCACCTCC CTGGGTCCCT TGGATCTGCT GCCCAGTGAG 
GAGCTGTTGA CAGACACAAG TAACTCCTCC TCGTCCACGG GGGAGGAAGC CGACCTGGCG 
GCCCTGCTGC CTGACCTCTC CGGCCGTCTC CTCATCAACT CCGTCTTCCA CGTGGGTGCT 
GAGCGGCTCC AGCAGATGCT CTTCTCAGAC TCGCCCTTCC TGCAGGACTT CCTGCAGCAG 
TGCAAGTTCA CAGACGTGAC CTTGAGCCCC TGGAGTGGGG ACAGCAAATG CCACCAGCGC 
CGAGTGCTGA CCTACACCAT TCCCATCAGC AACCCGCTGG GGCCCAAGAG CGCCTCTGTG 
GTGGAGACAC AGACGCTGTT CCGGCGTGGC CCCCAGGCGG GAGGGTGTGT GGTAGACTCC 
GAGGTGCTGA CGCAGGGCAT CCCTTACCAA GACTACTTCT ACACTGCCCA CCGCTACTGC 
ATCCTGGGCC TCGCCCGGAA CAAGGCCCGC CTCCGGGTGT CCTCCGAGAT CCGCTACCGG 
AAGCAGCCCT GGAGCCTCGT GAAGTCCCTG ATTGAGAAGA ACTCATGGAG TGGCATCGAA 
GATTACTTCC ACCACCTGGA GCGAGAACTC GCCAAGGCCG AGAAGCTGTC CCTGGAGGAA 
GGCGGGAAGG ACACGCGGGG CTTGCTGTCT GGCCTGCGCC GGCGGAAGCG GCCTCTGAGC 
TGGAGGGCTC ACGGTGATGG GACCCCACAC CCGGACCCTG ACCCCTGTGC CCGGACTGGC 
ATGCACACCT CGGGCTCCCT CAGCTCCCGC TTCTCGGAAC CGTCTATGGA CCAGGGCCCC 
GGGGCAGGCA CCCCCAGCGC CCTGGTTCTC ATCAGCATTG TCCTCATCGT CCTCATTGCC 
CTCAACGTCC TCCTCTTTTA CCGCCTCTGG TCCCTGGAGA GGACAGCTCA CACTTTTGAG 
TCCTGGCACA GTCTGGCCCT GGCCAAGGGT AAGTTCCCCC AGACGGCCAC GGAGTGGGCG 
GAGATCCTGG CCCTGCAGAA GCAGTTCCAC AGCGTCGAGG TGCACAAGTG GAGGCAGATC 
CTGCGGGCTT CTGTGGAGCT CCTGGACGAG ATGAAGTTCT CACTGGAGAA GCTACATCAA 
GGCATCACCG TCTCAGACCC TCCCTTCGAC TCCCAGCCAC AGCCTGACGA CAGCTTCTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_010814851.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813153.2 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.

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CloneID	OBa224861
Clone ID Related Accession (Same CDS sequence)	XM_010814851.3 , XM_010814850.3
Accession Version	XM_010814851.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACATA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CGGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGAGGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813153.2
CDS	129..2186
Translation	MVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_010814851.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814851.3

ORF Insert Sequence:

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACATA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CGGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGAGGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRAMD1A ( XM_010814850.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813152.2 Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa224861
Clone ID Related Accession (Same CDS sequence)	XM_010814851.3 , XM_010814850.3
Accession Version	XM_010814850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRAM domain-containing protein 1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACATA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CGGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGAGGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813152.2
CDS	259..2316
Translation	MVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISIQLKEVTCLKKEKTAKLIPNAIQICTENEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSPRELWHLVHQCYGSELGLTSEDEDYVCPLQLNGLGSPKDVGDVIALSDITPSGAADHSQEPSPAGSRRGRITPNLSRASSDADHGAEEDKEEQTDSQPDASSSQTVTPVAEPPSAEPTPPDGPTSLGPLDLLPSEELLTDTSNSSSSTGEEADLAALLPDLSGRLLINSVFHVGAERLQQMLFSDSPFLQDFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGPKSASVVETQTLFRRGPQAGGCVVDSEVLTQGIPYQDYFYTAHRYCILGLARNKARLRVSSEIRYRKQPWSLVKSLIEKNSWSGIEDYFHHLERELAKAEKLSLEEGGKDTRGLLSGLRRRKRPLSWRAHGDGTPHPDPDPCARTGMHTSGSLSSRFSEPSMDQGPGAGTPSALVLISIVICVSLIVLIALNVLLFYRLWSLERTAHTFESWHSLALAKGKFPQTATEWAEILALQKQFHSVEVHKWRQILRASVELLDEMKFSLEKLHQGITVSDPPFDSQPQPDDSFS

Target ORF information:

RefSeq Version	XM_010814850.3
Organism	Bos taurus(cattle)
Definition	Bos taurus GRAM domain containing 1A (GRAMD1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010814850.3

ORF Insert Sequence:

ATGGTGGAGA AGGGGTCGGA TAGCTCCTCA GAGAAGGGTG GGGTGCCTGG GACACCCAGC 
ACCCAGAGCC TGGGCAGCCG GAACTTCATC CGCAACAGCA AGAAGATGCA GAGCTGGTAC 
AGTATGCTGA GCCCCACATA CAAACAGCGG AACGAGGACT TCCGGAAACT GTTCAGCAAA 
CTCCCTGAAG CAGAACGCCT CATTGTGGAT TACTCCTGTG CCTTGCAGCG CGAGATCCTC 
CTCCAGGGCC GCCTCTATCT CTCCGAGAAC TGGATCTGCT TCTACAGCAA CATCTTCCGC 
TGGGAGACAA CCATCTCCAT CCAGTTGAAG GAAGTGACGT GTCTGAAGAA GGAAAAGACG 
GCCAAGCTGA TCCCCAATGC CATCCAGATC TGCACGGAGA ACGAGAAGCA TTTCTTCACC 
TCCTTTGGGG CCCGAGATCG CTGCTTCCTC CTCATCTTCC GCCTCTGGCA GAACGCGCTG 
CTTGAAAAGA CGCTGAGTCC CCGCGAGCTC TGGCACCTGG TGCATCAGTG CTACGGCTCA 
GAGCTGGGCC TCACCAGCGA GGACGAGGAC TATGTCTGCC CCTTACAGCT GAATGGCCTA 
GGGAGCCCCA AGGATGTGGG CGATGTAATT GCCCTGAGCG ACATCACCCC CTCGGGGGCA 
GCTGACCACA GCCAGGAGCC AAGCCCCGCA GGTTCACGCC GTGGCCGCAT CACCCCCAAC 
CTGTCCCGGG CCAGCAGTGA TGCAGACCAT GGGGCAGAGG AGGACAAGGA GGAGCAGACA 
GACAGCCAGC CAGATGCCTC CTCCAGCCAG ACAGTGACCC CGGTGGCTGA ACCCCCGAGC 
GCAGAGCCCA CCCCACCTGA CGGGCCCACC TCCCTGGGTC CCTTGGATCT GCTGCCCAGT 
GAGGAGCTGT TGACAGACAC AAGTAACTCC TCCTCGTCCA CGGGGGAGGA AGCCGACCTG 
GCGGCCCTGC TGCCTGACCT CTCCGGCCGT CTCCTCATCA ACTCCGTCTT CCACGTGGGT 
GCTGAGCGGC TCCAGCAGAT GCTCTTCTCA GACTCGCCCT TCCTGCAGGA CTTCCTGCAG 
CAGTGCAAGT TCACAGACGT GACCTTGAGC CCCTGGAGTG GGGACAGCAA ATGCCACCAG 
CGCCGAGTGC TGACCTACAC CATTCCCATC AGCAACCCGC TGGGGCCCAA GAGCGCCTCT 
GTGGTGGAGA CACAGACGCT GTTCCGGCGT GGCCCCCAGG CGGGAGGGTG TGTGGTAGAC 
TCCGAGGTGC TGACGCAGGG CATCCCTTAC CAAGACTACT TCTACACTGC CCACCGCTAC 
TGCATCCTGG GCCTCGCCCG GAACAAGGCC CGCCTCCGGG TGTCCTCCGA GATCCGCTAC 
CGGAAGCAGC CCTGGAGCCT CGTGAAGTCC CTGATTGAGA AGAACTCATG GAGTGGCATC 
GAAGATTACT TCCACCACCT GGAGCGAGAA CTCGCCAAGG CCGAGAAGCT GTCCCTGGAG 
GAAGGCGGGA AGGACACGCG GGGCTTGCTG TCTGGCCTGC GCCGGCGGAA GCGGCCTCTG 
AGCTGGAGGG CTCACGGTGA TGGGACCCCA CACCCGGACC CTGACCCCTG TGCCCGGACT 
GGCATGCACA CCTCGGGCTC CCTCAGCTCC CGCTTCTCGG AACCGTCTAT GGACCAGGGC 
CCCGGGGCAG GCACCCCCAG CGCCCTGGTT CTCATCAGCA TTGTGATCTG TGTGAGCCTC 
ATCGTCCTCA TTGCCCTCAA CGTCCTCCTC TTTTACCGCC TCTGGTCCCT GGAGAGGACA 
GCTCACACTT TTGAGTCCTG GCACAGTCTG GCCCTGGCCA AGGGTAAGTT CCCCCAGACG 
GCCACGGAGT GGGCGGAGAT CCTGGCCCTG CAGAAGCAGT TCCACAGCGT CGAGGTGCAC 
AAGTGGAGGC AGATCCTGCG GGCTTCTGTG GAGCTCCTGG ACGAGATGAA GTTCTCACTG 
GAGAAGCTAC ATCAAGGCAT CACCGTCTCA GACCCTCCCT TCGACTCCCA GCCACAGCCT 
GACGACAGCT TCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	GRAMD1A
Entrez Gene ID	507027
Full Name	GRAM domain containing 1A
General protein information	Preferred Names GRAM domain containing 1A Names GRAM domain-containing protein 1A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Canis lupus familiaris (dog)	GRAMD1A	XP_005616784.1
Bos taurus (cattle)	GRAMD1A	XP_002694974.1
Rattus norvegicus (Norway rat)	Gramd1a	NP_001014182.1
Pan troglodytes (chimpanzee)	GRAMD1A	XP_001157243.2
Mus musculus (house mouse)	Gramd1a	NP_082174.3
Xenopus tropicalis (tropical clawed frog)	gramd1a	XP_002940577.2
Danio rerio (zebrafish)	gramd1a	XP_683776.6
Homo sapiens (human)	GRAMD1A	NP_065946.2

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GRAMD1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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