ZAP70 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZAP70
Entrez Gene ID	504509
Full Name	zeta chain of T-cell receptor associated protein kinase 70
General protein information	Preferred Names zeta chain of T-cell receptor associated protein kinase 70 Names tyrosine-protein kinase ZAP-70 zeta-chain (TCR) associated protein kinase 70kDa
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015473327.1	XP_015328813.1	tyrosine-protein kinase ZAP-70 isoform X1
XM_005212295.3	XP_005212352.1	tyrosine-protein kinase ZAP-70 isoform X1
NM_001193017.2	NP_001179946.1	tyrosine-protein kinase ZAP-70
XM_005212295.4	XP_005212352.1	tyrosine-protein kinase ZAP-70 isoform X1
NM_001193017.3	NP_001179946.1	tyrosine-protein kinase ZAP-70

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZAP70	NP_001070.2
Canis lupus familiaris (dog)	ZAP70	XP_005626103.1
Gallus gallus (chicken)	ZAP70	XP_418206.4
Xenopus tropicalis (tropical clawed frog)	zap70	NP_001006825.1
	ZAP70	XP_001101570.2
Pan troglodytes (chimpanzee)	ZAP70	XP_515637.2
Mus musculus (house mouse)	Zap70	NP_033565.2
Rattus norvegicus (Norway rat)	Zap70	NP_001012002.1
Danio rerio (zebrafish)	zap70	NP_001018425.1
Bos taurus (cattle)	ZAP70	NP_001179946.1

Gene Ontology
Bibliography

Related articles in PubMed

ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
Leite FL, Eslab?o LB, Pesch B, Bannantine JP, Reinhardt TA, Stabel JR
Veterinary immunology and immunopathology167(1-2)15-21(2015 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The surface expression of CTLA-4 was increased in subclinical stages of paratuberculosis infection while levels of ZAP-70 were decreased in CD4+ T cells of both subclinical and clinical animals, indicating a change in T cell phenotype with disease state.
Title: ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.

The following ZAP70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZAP70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa38429	NM_001193017.2 Latest version!	Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa23675	XM_015473327.1 Latest version!	Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa23675	XM_005212295.3 Latest version!	Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa220178	XM_005212295.4 Latest version!	Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OBa239166	NM_001193017.3 Latest version!	Bos taurus zeta chain of T cell receptor associated protein kinase 70 (ZAP70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZAP70 ( NM_001193017.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179946.1 Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), mRNA.

Price & Availability↑

CloneID	OBa38429
Clone ID Related Accession (Same CDS sequence)	NM_001193017.2
Accession Version	NM_001193017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase ZAP-70
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02030134.1. On Feb 23, 2016 this sequence version replaced NM_001193017.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC142505.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822090 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGACC CCGCGGCGCA CCTGCCCTTC TTCTACGGCA GCATCTCGCG CGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC GGGCATGGCC GACGGGCTCT TCCTGCTGCG GCAGTGCCTG 
CGCTCGCTGG GCGGCTATGT GCTGTCGCTC GTGCACGAAG TGCGCTTCCA CCACTTCCCC 
ATCGAGCGCC AGCTCAACGG CACCTACGCT ATCGCGGGAG GAAAGGCGCA CTGCGGCCCG 
GCTGAGCTCT GCGAGTTCTA TTCACGCGAC CCCGACGGGC TGCCCTGCAA CCTGCGCAAG 
CCGTGCAACC GGCCGTCGGG GCTCGAGCCG CAGCCCGGGG TATTTGACAA CCTTCGCGAC 
GCCATGGTGC GCGACTACGT GCGCCAGACT TGGAAGCTGG AGGGCGAGGC CTTGGAGCAA 
GCCATCATCA GCCAGGCACC CCAGGTGGAG AAGCTTATTG CCACGACAGC TCACGAACGG 
ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179946.1
CDS	163..2016
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHEVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDNLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGSQTDGKFLLRPRKEPGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASSGAAAPTLPAHPSTFTQPQRRIDTLNSDGYTPEPARLVSSEKPRTMPMDTSVYESPYSDPEELKNKKLFLKRENLLMADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKKEEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPEMYKLMSDCWTYKWEDRPDFAAVEQRMRTYYYSLATKAEEPAACGNGVEAACP

Target ORF information:

RefSeq Version	NM_001193017.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193017.2

ORF Insert Sequence:

ATGCCGGACC CCGCGGCGCA CCTGCCCTTC TTCTACGGCA GCATCTCGCG CGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC GGGCATGGCC GACGGGCTCT TCCTGCTGCG GCAGTGCCTG 
CGCTCGCTGG GCGGCTATGT GCTGTCGCTC GTGCACGAAG TGCGCTTCCA CCACTTCCCC 
ATCGAGCGCC AGCTCAACGG CACCTACGCT ATCGCGGGAG GAAAGGCGCA CTGCGGCCCG 
GCTGAGCTCT GCGAGTTCTA TTCACGCGAC CCCGACGGGC TGCCCTGCAA CCTGCGCAAG 
CCGTGCAACC GGCCGTCGGG GCTCGAGCCG CAGCCCGGGG TATTTGACAA CCTTCGCGAC 
GCCATGGTGC GCGACTACGT GCGCCAGACT TGGAAGCTGG AGGGCGAGGC CTTGGAGCAA 
GCCATCATCA GCCAGGCACC CCAGGTGGAG AAGCTTATTG CCACGACAGC TCACGAACGG 
ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAP70 ( XM_015473327.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328813.1 Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23675
Clone ID Related Accession (Same CDS sequence)	XM_015473327.1 , XM_005212295.3
Accession Version	XM_015473327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328813.1
CDS	240..1613
Translation	MPWYHSSLTREEAERKLYSGSQTDGKFLLRPRKEPGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASSGAAAPTLPAHPSTFTQPQRRIDTLNSDGYTPEPARLVSSEKPRTMPMDTSVYESPYSDPEELKNKKLFLKRENLLMADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKKEEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPEMYKLMSDCWTYKWEDRPDFAAVEQRMRTYYYSLATKAEEPAACGNGVEAACP

Target ORF information:

RefSeq Version	XM_015473327.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473327.1

ORF Insert Sequence:

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAP70 ( XM_005212295.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212352.1 Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23675
Clone ID Related Accession (Same CDS sequence)	XM_015473327.1 , XM_005212295.3
Accession Version	XM_005212295.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104286.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212295.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212352.1
CDS	304..1677
Translation	MPWYHSSLTREEAERKLYSGSQTDGKFLLRPRKEPGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASSGAAAPTLPAHPSTFTQPQRRIDTLNSDGYTPEPARLVSSEKPRTMPMDTSVYESPYSDPEELKNKKLFLKRENLLMADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKKEEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPEMYKLMSDCWTYKWEDRPDFAAVEQRMRTYYYSLATKAEEPAACGNGVEAACP

Target ORF information:

RefSeq Version	XM_005212295.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212295.3

ORF Insert Sequence:

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTACGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAP70 ( XM_005212295.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212352.1 Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), transcript variant X1, mRNA.

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CloneID	OBa220178
Clone ID Related Accession (Same CDS sequence)	XM_005212295.4
Accession Version	XM_005212295.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase ZAP-70 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212295.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTATGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212352.1
CDS	237..1610
Translation	MPWYHSSLTREEAERKLYSGSQTDGKFLLRPRKEPGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASSGAAAPTLPAHPSTFTQPQRRIDTLNSDGYTPEPARLVSSEKPRTMPMDTSVYESPYSDPEELKNKKLFLKRENLLMADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKKEEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPEMYKLMSDCWTYKWEDRPDFAAVEQRMRTYYYSLATKAEEPAACGNGVEAACP

Target ORF information:

RefSeq Version	XM_005212295.4
Organism	Bos taurus(cattle)
Definition	Bos taurus zeta chain of T-cell receptor associated protein kinase 70 (ZAP70), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212295.4

ORF Insert Sequence:

ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTATGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZAP70 ( NM_001193017.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179946.1 Bos taurus zeta chain of T cell receptor associated protein kinase 70 (ZAP70), mRNA.

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CloneID	OBa239166
Clone ID Related Accession (Same CDS sequence)	NM_001193017.3
Accession Version	NM_001193017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bos taurus(cattle)
Product	tyrosine-protein kinase ZAP-70
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On May 29, 2018 this sequence version replaced NM_001193017.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC142505.1, SRR6764281.30268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145420, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGACC CCGCGGCGCA CCTGCCCTTC TTCTACGGCA GCATCTCGCG CGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC GGGCATGGCC GACGGGCTCT TCCTGCTGCG GCAGTGCCTG 
CGCTCGCTGG GCGGCTATGT GCTGTCGCTC GTGCACGAAG TGCGCTTCCA CCACTTCCCC 
ATCGAGCGCC AGCTCAACGG CACCTACGCT ATCGCGGGAG GAAAGGCGCA CTGCGGCCCG 
GCTGAGCTCT GCGAGTTCTA TTCACGCGAC CCCGACGGGC TGCCCTGCAA CCTGCGCAAG 
CCGTGCAACC GGCCGTCGGG GCTCGAGCCG CAGCCCGGGG TATTTGACAA CCTTCGCGAC 
GCCATGGTGC GCGACTACGT GCGCCAGACT TGGAAGCTGG AGGGCGAGGC CTTGGAGCAA 
GCCATCATCA GCCAGGCACC CCAGGTGGAG AAGCTTATTG CCACGACAGC TCACGAACGG 
ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTATGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179946.1
CDS	163..2016
Translation	MPDPAAHLPFFYGSISRAEAEEHLKLAGMADGLFLLRQCLRSLGGYVLSLVHEVRFHHFPIERQLNGTYAIAGGKAHCGPAELCEFYSRDPDGLPCNLRKPCNRPSGLEPQPGVFDNLRDAMVRDYVRQTWKLEGEALEQAIISQAPQVEKLIATTAHERMPWYHSSLTREEAERKLYSGSQTDGKFLLRPRKEPGTYALSLIYGKTVYHYLISQDKAGKYCIPEGTKFDTLWQLVEYLKLKADGLIYCLKEACPNSSASSGAAAPTLPAHPSTFTQPQRRIDTLNSDGYTPEPARLVSSEKPRTMPMDTSVYESPYSDPEELKNKKLFLKRENLLMADIELGCGNFGSVRQGVYRMRKKQIDVAIKVLKQSTEKADKDEMMREAQIMHQLDNPYIVRLIGVCQAEALMLVMEMAGGGPLHKFLVGKKEEIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNRHYAKISDFGLSKALGADDSYYTARSAGKWPLKWYAPECINFRKFSSRSDVWSYGVTMWEAFSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPEMYKLMSDCWTYKWEDRPDFAAVEQRMRTYYYSLATKAEEPAACGNGVEAACP

Target ORF information:

RefSeq Version	NM_001193017.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zeta chain of T cell receptor associated protein kinase 70 (ZAP70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193017.3

ORF Insert Sequence:

ATGCCGGACC CCGCGGCGCA CCTGCCCTTC TTCTACGGCA GCATCTCGCG CGCCGAGGCC 
GAGGAGCACC TGAAGCTGGC GGGCATGGCC GACGGGCTCT TCCTGCTGCG GCAGTGCCTG 
CGCTCGCTGG GCGGCTATGT GCTGTCGCTC GTGCACGAAG TGCGCTTCCA CCACTTCCCC 
ATCGAGCGCC AGCTCAACGG CACCTACGCT ATCGCGGGAG GAAAGGCGCA CTGCGGCCCG 
GCTGAGCTCT GCGAGTTCTA TTCACGCGAC CCCGACGGGC TGCCCTGCAA CCTGCGCAAG 
CCGTGCAACC GGCCGTCGGG GCTCGAGCCG CAGCCCGGGG TATTTGACAA CCTTCGCGAC 
GCCATGGTGC GCGACTACGT GCGCCAGACT TGGAAGCTGG AGGGCGAGGC CTTGGAGCAA 
GCCATCATCA GCCAGGCACC CCAGGTGGAG AAGCTTATTG CCACGACAGC TCACGAACGG 
ATGCCCTGGT ACCACAGCAG CCTGACGCGC GAGGAGGCTG AGCGCAAACT CTACTCGGGC 
TCCCAGACCG ACGGCAAGTT CCTGCTGAGA CCCCGGAAAG AGCCGGGCAC GTACGCGCTG 
TCTCTGATCT ATGGGAAAAC TGTGTACCAC TACCTCATCA GCCAGGACAA GGCGGGCAAA 
TACTGCATTC CCGAGGGGAC CAAATTTGAC ACACTCTGGC AGCTGGTGGA GTACCTGAAG 
CTGAAGGCTG ATGGACTCAT CTACTGCCTG AAGGAGGCCT GTCCCAACAG CAGCGCCAGC 
TCAGGGGCCG CTGCTCCTAC ACTCCCTGCC CACCCGTCCA CATTCACCCA GCCTCAGAGA 
CGGATCGACA CTCTCAACTC AGATGGATAC ACCCCTGAAC CAGCGCGGTT AGTGTCCTCA 
GAGAAGCCGC GAACGATGCC CATGGACACC AGCGTCTATG AGAGCCCCTA CAGCGACCCT 
GAGGAGCTCA AAAACAAGAA GCTCTTCCTG AAGCGTGAGA ACCTCCTCAT GGCTGACATC 
GAACTTGGCT GCGGCAACTT CGGTTCAGTT CGCCAGGGCG TCTACCGTAT GCGCAAGAAG 
CAGATCGACG TGGCCATCAA GGTGCTGAAA CAGAGCACGG AGAAGGCGGA CAAGGACGAG 
ATGATGCGGG AGGCACAGAT CATGCACCAG CTGGACAACC CCTACATCGT GCGGCTCATT 
GGAGTCTGCC AGGCCGAGGC CCTCATGCTG GTCATGGAGA TGGCTGGTGG CGGGCCCCTG 
CATAAGTTCC TGGTCGGGAA GAAGGAGGAG ATCCCCGTCA GCAATGTGGC GGAGCTGCTG 
CACCAGGTAT CTATGGGGAT GAAGTACCTG GAAGAGAAAA ACTTTGTGCA TCGTGACCTG 
GCGGCCCGCA ATGTCCTGCT GGTCAACCGG CACTATGCCA AGATCAGCGA CTTCGGGCTC 
TCCAAGGCCC TGGGTGCCGA CGACAGCTAC TACACCGCCC GCTCAGCCGG GAAGTGGCCG 
CTCAAGTGGT ATGCGCCCGA GTGCATCAAC TTCCGCAAGT TCTCCAGCCG CAGCGACGTC 
TGGAGCTACG GAGTCACCAT GTGGGAGGCC TTCTCCTATG GGCAGAAGCC CTACAAGAAG 
ATGAAGGGCC CGGAGGTCAT GGCCTTCATC GAGCAGGGCA AGCGGATGGA GTGCCCACCA 
GAGTGTCCCC CTGAGATGTA CAAGCTCATG AGCGATTGCT GGACCTACAA GTGGGAGGAC 
CGCCCGGACT TCGCCGCAGT TGAACAGCGC ATGCGCACCT ACTACTACAG CTTGGCGACC 
AAGGCAGAGG AGCCCGCCGC TTGTGGAAAC GGGGTTGAGG CTGCGTGTCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZAP-70, CTLA-4 and proximal T cell receptor signaling in cows infected with Mycobacterium avium subsp. paratuberculosis.
Veterinary immunology and immunopathology167(1-2)15-21(2015 Sep)
Leite FL,Eslab?o LB,Pesch B,Bannantine JP,Reinhardt TA,Stabel JR

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ZAP70 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

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