NDUFB2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFB2
Entrez Gene ID	327713
Full Name	NADH:ubiquinone oxidoreductase subunit B2
Synonyms	AGGG
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit B2 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial CI-AGGG NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa NADH-ubiquinone oxidoreductase AGGG subunit complex I-AGGG
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_175828.2	NP_787022.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial precursor
XM_024990498.1	XP_024846266.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NDUFB2	NP_004537.1
	NDUFB2	XP_001082389.1
Mus musculus (house mouse)	Ndufb2	NP_080888.1
Canis lupus familiaris (dog)	NDUFB2	XP_003432113.1
Rattus norvegicus (Norway rat)	Ndufb2	NP_001102094.2
Gallus gallus (chicken)	NDUFB2	NP_001264825.1
Bos taurus (cattle)	NDUFB2	NP_787022.1
Pan troglodytes (chimpanzee)	NDUFB2	NP_001065266.1
Danio rerio (zebrafish)	zgc:73329	NP_001161298.1
Xenopus tropicalis (tropical clawed frog)	ndufb2	NP_001106529.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusov? E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Walker JE, Arizmendi JM, Dupuis A, Fearnley IM, Finel M, Medd SM, Pilkington SJ, Runswick MJ, Skehel JM
Journal of molecular biology226(4)1051-72(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa18824	NM_175828.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa233562	XM_024990498.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFB2 ( NM_175828.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_787022.1 Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), mRNA.

Price & Availability↑

CloneID	OBa18824
Clone ID Related Accession (Same CDS sequence)	NM_175828.2
Accession Version	NM_175828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63216.1. On Jun 3, 2003 this sequence version replaced NM_175828.1. ##Evidence-Data-START## Transcript exon combination :: X63216.1, EH202703.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGGCA TGTCGGCTCT GAAGCGACTG GCGCCGTTCG CTCACGTTGG AGGCCACCTC 
TTCAGAGGCC GCTGCGCGAG GGCGGCTGGA GCCCGTGGCG TCCGTCGGGC TGGCGGAGGT 
GCGCACATTG AGCCCCGGTA CAGACAGTTC CCGCAGCTGA CCAGATCCCA GGTGATCCAG 
GCCGAGTTCT TCAGTGCGAC CATGTGGTTC TGGATTCTCT GGCGCTTCTG GCACGACTCA 
GATGCTGTGC TGGGTCACTT TCCTTATCCC GACCCTTCCC AGTGGACAGA TGAGGAATTG 
GGTATCCCTC CGGATGATGA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787022.1
CDS	37..363
Translation	MAGMSALKRLAPFAHVGGHLFRGRCARAAGARGVRRAGGGAHIEPRYRQFPQLTRSQVIQAEFFSATMWFWILWRFWHDSDAVLGHFPYPDPSQWTDEELGIPPDDED

Target ORF information:

RefSeq Version	NM_175828.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175828.2

ORF Insert Sequence:

ATGGCTGGCA TGTCGGCTCT GAAGCGACTG GCGCCGTTCG CTCACGTTGG AGGCCACCTC 
TTCAGAGGCC GCTGCGCGAG GGCGGCTGGA GCCCGTGGCG TCCGTCGGGC TGGCGGAGGT 
GCGCACATTG AGCCCCGGTA CAGACAGTTC CCGCAGCTGA CCAGATCCCA GGTGATCCAG 
GCCGAGTTCT TCAGTGCGAC CATGTGGTTC TGGATTCTCT GGCGCTTCTG GCACGACTCA 
GATGCTGTGC TGGGTCACTT TCCTTATCCC GACCCTTCCC AGTGGACAGA TGAGGAATTG 
GGTATCCCTC CGGATGATGA AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFB2 ( XM_024990498.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846266.1 Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233562
Clone ID Related Accession (Same CDS sequence)	XM_024990498.1
Accession Version	XM_024990498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA TGTCGGCTCT GAAGCGACTG GCGCCGTTCG CTCACGTTGG AGGCCACCTC 
TTCAGAGGCC GCTGCGCGAG GGCGGCTGGA GCCCGTGGCG TCCGTCGGGC TGGCGGAGGT 
GCGCACATTG AGCCCCGGTA CAGACAGTTC CCGCAGCTGA CCAGATCCCA GGTGATCCAG 
GCCGAGTTCT TCAGTGCGAC CATGTGGTTC TGGATTCTCT GGCGCTTCTG GCACGACTCA 
GATGCTGTGC TGAATTGCTG TGCCCTCGTC CCCCAGAAAG AAGCTGCCTG GCGTCTGTGG 
ACTGGCAGCA CACAGACACC TTTGTCTCAC TGTGGGTCGG CTGTCAGATC TGTGTGTGGC 
TGTCTGGCAG GAGATGATGG CAGGCCCTGG ATGAGGATCA AAGCGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846266.1
CDS	62..472
Translation	MAGMSALKRLAPFAHVGGHLFRGRCARAAGARGVRRAGGGAHIEPRYRQFPQLTRSQVIQAEFFSATMWFWILWRFWHDSDAVLNCCALVPQKEAAWRLWTGSTQTPLSHCGSAVRSVCGCLAGDDGRPWMRIKAE

Target ORF information:

RefSeq Version	XM_024990498.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B2 (NDUFB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990498.1

ORF Insert Sequence:

ATGGCTGGCA TGTCGGCTCT GAAGCGACTG GCGCCGTTCG CTCACGTTGG AGGCCACCTC 
TTCAGAGGCC GCTGCGCGAG GGCGGCTGGA GCCCGTGGCG TCCGTCGGGC TGGCGGAGGT 
GCGCACATTG AGCCCCGGTA CAGACAGTTC CCGCAGCTGA CCAGATCCCA GGTGATCCAG 
GCCGAGTTCT TCAGTGCGAC CATGTGGTTC TGGATTCTCT GGCGCTTCTG GCACGACTCA 
GATGCTGTGC TGAATTGCTG TGCCCTCGTC CCCCAGAAAG AAGCTGCCTG GCGTCTGTGG 
ACTGGCAGCA CACAGACACC TTTGTCTCAC TGTGGGTCGG CTGTCAGATC TGTGTGTGGC 
TGTCTGGCAG GAGATGATGG CAGGCCCTGG ATGAGGATCA AAGCGGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusov? E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Journal of molecular biology226(4)1051-72(1992 Aug)
Walker JE,Arizmendi JM,Dupuis A,Fearnley IM,Finel M,Medd SM,Pilkington SJ,Runswick MJ,Skehel JM