NDUFC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFC1
Entrez Gene ID	282289
Full Name	NADH:ubiquinone oxidoreductase subunit C1
Synonyms	CI-KFYI
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit C1 Names NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1 (6kD, KFYI) NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa NADH-ubiquinone oxidoreductase KFYI subunit complex I-KFYI
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174564.2	NP_776989.1	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial precursor
XM_005217536.2	XP_005217593.1	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial isoform X1
XM_024977022.1	XP_024832790.1	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NDUFC1	NP_776989.1
Homo sapiens (human)	NDUFC1	NP_002485.1
	NDUFC1	XP_001087956.2
Canis lupus familiaris (dog)	NDUFC1	XP_005631837.1
Mus musculus (house mouse)	Ndufc1	NP_079799.1
Rattus norvegicus (Norway rat)	Ndufc1	XP_003749323.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusov? E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Walker JE, Arizmendi JM, Dupuis A, Fearnley IM, Finel M, Medd SM, Pilkington SJ, Runswick MJ, Skehel JM
Journal of molecular biology226(4)1051-72(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa27653	NM_174564.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa27653	XM_005217536.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa224154	XM_024977022.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFC1 ( NM_174564.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776989.1 Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), mRNA.

Price & Availability↑

CloneID	OBa27653
Clone ID Related Accession (Same CDS sequence)	NM_174564.2 , XM_005217536.2
Accession Version	NM_174564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63214.1. On Jun 3, 2003 this sequence version replaced NM_174564.1. ##Evidence-Data-START## Transcript exon combination :: X63214.1, DV802187.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776989.1
CDS	19..249
Translation	MAPSALLRPFWKLLAPARFPSVSSSRSKFYIQEPPHGSPNWLKVGLTLGTSVFLWIYLIKQHNEDVLEYKRRNGLE

Target ORF information:

RefSeq Version	NM_174564.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174564.2

ORF Insert Sequence:

ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFC1 ( XM_005217536.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005217593.1 Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27653
Clone ID Related Accession (Same CDS sequence)	NM_174564.2 , XM_005217536.2
Accession Version	XM_005217536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104447.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005217536.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005217593.1
CDS	715..945
Translation	MAPSALLRPFWKLLAPARFPSVSSSRSKFYIQEPPHGSPNWLKVGLTLGTSVFLWIYLIKQHNEDVLEYKRRNGLE

Target ORF information:

RefSeq Version	XM_005217536.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005217536.2

ORF Insert Sequence:

ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFC1 ( XM_024977022.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832790.1 Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224154
Clone ID Related Accession (Same CDS sequence)	XM_024977022.1
Accession Version	XM_024977022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGTG GCCTCCGAGC CACACAGTTT CTCGCCTCCA CCCGCCAGGG GGCGCTCGGC 
CCAACGTCGG CCCCGGGAGC TGCGCGGCTG GAGGAGGCGG AGCCCAGGAA GCCAGGCAAG 
ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832790.1
CDS	714..1064
Translation	MQRGLRATQFLASTRQGALGPTSAPGAARLEEAEPRKPGKMAPSALLRPFWKLLAPARFPSVSSSRSKFYIQEPPHGSPNWLKVGLTLGTSVFLWIYLIKQHNEDVLEYKRRNGLE

Target ORF information:

RefSeq Version	XM_024977022.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977022.1

ORF Insert Sequence:

ATGCAGCGTG GCCTCCGAGC CACACAGTTT CTCGCCTCCA CCCGCCAGGG GGCGCTCGGC 
CCAACGTCGG CCCCGGGAGC TGCGCGGCTG GAGGAGGCGG AGCCCAGGAA GCCAGGCAAG 
ATGGCGCCGT CCGCGTTGTT GCGGCCATTC TGGAAGCTGC TGGCCCCGGC TAGGTTCCCG 
AGTGTCTCTT CATCTCGGTC AAAGTTCTAC ATTCAGGAAC CGCCACATGG CAGTCCCAAC 
TGGCTGAAAG TTGGCTTGAC CTTGGGCACC TCCGTTTTTT TGTGGATCTA TCTCATCAAA 
CAACATAACG AAGATGTTTT AGAGTATAAA AGAAGAAATG GGTTGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusov? E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Journal of molecular biology226(4)1051-72(1992 Aug)
Walker JE,Arizmendi JM,Dupuis A,Fearnley IM,Finel M,Medd SM,Pilkington SJ,Runswick MJ,Skehel JM