HIF1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	281814
Full Name	hypoxia inducible factor 1 alpha subunit
General protein information	Preferred Names hypoxia inducible factor 1 alpha subunit Names hypoxia-inducible factor 1-alpha HIF-1-alpha HIF1-alpha hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174339.3	NP_776764.2	hypoxia-inducible factor 1-alpha
XM_024997269.1	XP_024853037.1	hypoxia-inducible factor 1-alpha isoform X1
XM_024997270.1	XP_024853038.1	hypoxia-inducible factor 1-alpha isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary.
Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW
Reproduction in domestic animals = Zuchthygiene52(1)130-139(2017 Feb)

Glucose transport and milk secretion during manipulated plasma insulin and glucose concentrations and during LPS-induced mastitis in dairy cows.
Gross JJ, van Dorland HA, Wellnitz O, Bruckmaier RM
Journal of animal physiology and animal nutrition99(4)747-56(2015 Aug)

Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle.
Mattmiller SA, Corl CM, Gandy JC, Loor JJ, Sordillo LM
Journal of dairy science94(6)2912-22(2011 Jun)

Hypoxia is important for establishing vascularization during corpus luteum formation in cattle.
Nishimura R, Okuda K
The Journal of reproduction and development56(1)110-6(2010 Feb)

Sphingosine-1-phosphate: a novel nonhypoxic activator of hypoxia-inducible factor-1 in vascular cells.
Michaud MD, Robitaille GA, Gratton JP, Richard DE
Arteriosclerosis, thrombosis, and vascular biology29(6)902-8(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

inverse expression and localization pattern of HIF1A and vasohibins during different stages of ovarian function in cow
Title: Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary.

changes in hypoxia-associated gene expression during the lactation cycle
Title: Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle.

Hypoxia increased the amounts of HIF1A protein, VEGF mRNA and VEGF protein in cultured bovine luteal cells.
Title: Hypoxia is important for establishing vascularization during corpus luteum formation in cattle.

Identify sphingosine-1-phosphate as a novel and potent nonhypoxic activator of HIF-1.
Title: Sphingosine-1-phosphate: a novel nonhypoxic activator of hypoxia-inducible factor-1 in vascular cells.

VEGF has an effect on the expression of its own transcription factor, HIF-1, and on VEGF itself
Title: Induction of hypoxia-inducible factor 1alpha gene expression by vascular endothelial growth factor.

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43417	NM_174339.3 Latest version!	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa219407	XM_024997269.1 Latest version!	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa219408	XM_024997270.1 Latest version!	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( NM_174339.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776764.2 Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Price & Availability↑

CloneID	OBa43417
Clone ID Related Accession (Same CDS sequence)	NM_174339.3
Accession Version	NM_174339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126622.1. On Nov 13, 2006 this sequence version replaced NM_174339.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTCACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CACGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGAC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCTATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776764.2
CDS	280..2751
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_174339.3
Organism	Bos taurus(cattle)
Definition	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174339.3

ORF Insert Sequence:

ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTCACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CACGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA 
TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT 
GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACAACAGGAC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT 
CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT 
GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCTAAT 
GAGAAATTGC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT 
GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG 
CCCACTCAAA TGCAAGAACC TCCTATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA 
TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT 
CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA 
AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT 
CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG 
GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA 
CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT 
CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_024997269.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853037.1 Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219407
Clone ID Related Accession (Same CDS sequence)	XM_024997269.1
Accession Version	XM_024997269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT 
CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT 
TATGAGCTTG CTCATCAGTT GCCACTCCCC CATAATGTAA GCTCGCATCT TGATAAGGCT 
TCTGTTATGA GGCTCACCAT CAGCTATTTG CGTGTGAGGA AACTTCTGGA TGCTGGTGAT 
TTGGATATTG AAGATGAAAT GAAGGCACAG ATGAATTGCT TTTATTTGAA AGCCTTGGAT 
GGTTTTGTTA TGGTTCTCAC AGATGATGGT GACATGATTT ACATTTCTGA TAATGTGAAC 
AAATACATGG GATTAACTCA GTTTGAACTA ACTGGACACA GTGTGTTTGA TTTTACTCAT 
CCATGTGACC ACGAGGAAAT GAGAGAAATG CTTACACACA GAAATGGCCT TGTGAAAAAG 
GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC 
CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT 
ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG 
ACGTGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA 
GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA 
AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA 
TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA 
GGACAAGTCA CAACAGGACA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA 
GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT 
GTAAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA 
GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA 
GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA 
ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC 
ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAATGATGT AATGCTCCCC 
TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCAATGT CTCCATTACC TGCCTCTGAA 
ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG 
TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG 
CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT 
GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG 
AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA 
GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC 
GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA 
GTATTCCAGC CCACTCAAAT GCAAGAACCT CCTATAGCCA CCGTCACTAC CACAGCCACC 
AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA 
TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT 
GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT 
GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA 
CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853037.1
CDS	1125..3605
Translation	MLGRSLTYCSVLSKWISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_024997269.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997269.1

ORF Insert Sequence:

ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT 
CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT 
TATGAGCTTG CTCATCAGTT GCCACTCCCC CATAATGTAA GCTCGCATCT TGATAAGGCT 
TCTGTTATGA GGCTCACCAT CAGCTATTTG CGTGTGAGGA AACTTCTGGA TGCTGGTGAT 
TTGGATATTG AAGATGAAAT GAAGGCACAG ATGAATTGCT TTTATTTGAA AGCCTTGGAT 
GGTTTTGTTA TGGTTCTCAC AGATGATGGT GACATGATTT ACATTTCTGA TAATGTGAAC 
AAATACATGG GATTAACTCA GTTTGAACTA ACTGGACACA GTGTGTTTGA TTTTACTCAT 
CCATGTGACC ACGAGGAAAT GAGAGAAATG CTTACACACA GAAATGGCCT TGTGAAAAAG 
GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC 
CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT 
ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG 
ACGTGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA 
GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA 
AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA 
TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA 
GGACAAGTCA CAACAGGACA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA 
GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT 
GTAAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA 
GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA 
GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA 
ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC 
ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAATGATGT AATGCTCCCC 
TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCAATGT CTCCATTACC TGCCTCTGAA 
ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG 
TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG 
CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT 
GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG 
AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA 
GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC 
GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA 
GTATTCCAGC CCACTCAAAT GCAAGAACCT CCTATAGCCA CCGTCACTAC CACAGCCACC 
AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA 
TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT 
GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT 
GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA 
CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( XM_024997270.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853038.1 Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

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CloneID	OBa219408
Clone ID Related Accession (Same CDS sequence)	XM_024997270.1
Accession Version	XM_024997270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA 
GAGAAGTCTA GAGATGCAGC CAGATCTCGT CGAAGTAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT CCCCCATAAT GTAAGCTCGC ATCTTGATAA GGCTTCTGTT 
ATGAGGCTCA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCACGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAATC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATACGATA CCAACAGTAA CCAATCTCAG TGTGGGTATA AGAAACCACC GATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAATTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCCTTGG ACTCTGACCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACAACAG GACAGTACAG AATGCTTGCC AAAAGAGGTG GATATGTCTG GATAGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAGCCCC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTTCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGCAGTCT CTTTGATAAA CTTAAGAAGG AGCCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAG 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCT 
AATGAGAAAT TGCAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAACCAAG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTT ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGTCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ATACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGATCAG 
TTGTCACCAC TGGAAAACAG TTCTACAAGT CCTCAGAGTG CAAGCACAAA TACAGTATTC 
CAGCCCACTC AAATGCAAGA ACCTCCTATA GCCACCGTCA CTACCACAGC CACCAGTGAT 
GAATTGAAAA CAGTGACAAA AGATGGTATG GAAGACATTA AAATACTGAT TGCATTTCCA 
TCTCCTCCCC ACGTACCTAA AGAACCTCCT TGTGCCACAA CGTCACCATA TAGTGATACT 
GGAAGTCGGA CAGCCTCACC CAACAGAGCA GGAAAAGGAG TCATAGAACA GACAGAAAAA 
TCTCATCCAA GAAGCCCTAA TGTGTTATCT GTCGCTTTGA GTCAAAGAAC TACTGCTCCT 
GAGGAAGAAC TAAATCCAAA GATACTAGCT TTGCAGAATG CTCAGAGAAA GCGAAAAATT 
GAACATGATG GTTCACTTTT TCAAGCAGTA GGAATTGGAA CATTATTACA GCAACCAGAT 
GATCGTGCAA CTACCACATC ACTTTCTTGG AAACGTGTAA AAGGATGCAA ATCTAGTGAA 
CAAAATGGAA TGGAGCAAAA GACAATTATT TTAATACCCT CTGATTTAGC ATGTAGACTG 
CTGGGGCAAT CAATGGATGA GAGTGGACTA CCACAGCTGA CCAGTTATGA TTGTGAAGTT 
AATGCTCCTA TACAAGGCAG CAGAAACCTA CTGCAGGGTG AAGAATTACT CAGAGCTTTG 
GATCAAGTTA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853038.1
CDS	65..2539
Translation	MAWDANFFVCTCRISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWIETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLENSSTSPQSASTNTVFQPTQMQEPPIATVTTTATSDELKTVTKDGMEDIKILIAFPSPPHVPKEPPCATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTAPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_024997270.1
Organism	Bos taurus(cattle)
Definition	Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997270.1

ORF Insert Sequence:

ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA 
GAGAAGTCTA GAGATGCAGC CAGATCTCGT CGAAGTAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT CCCCCATAAT GTAAGCTCGC ATCTTGATAA GGCTTCTGTT 
ATGAGGCTCA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT 
ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT 
GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC 
ATGGGATTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT 
GACCACGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA 
GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG 
AGAACTATGA ACATAAAATC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT 
GTATACGATA CCAACAGTAA CCAATCTCAG TGTGGGTATA AGAAACCACC GATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC 
AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAATTTT CTTATTGTGA TGAAAGAATT 
ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT 
CATGCCTTGG ACTCTGACCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA 
GTCACAACAG GACAGTACAG AATGCTTGCC AAAAGAGGTG GATATGTCTG GATAGAAACT 
CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAGCCCC AGTGCATTGT ATGTGTAAAT 
TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT 
GTTCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA 
TCAGAAGATA CAAGCAGTCT CTTTGATAAA CTTAAGAAGG AGCCTGATGC TTTAACTTTG 
CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAG 
ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCT 
AATGAGAAAT TGCAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA 
AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAACCAAG AAGTTGCATT AAAGTTAGAA 
CCAAATCCAG AGTCACTGGA ACTTTCTTTT ACCATGCCCC AGATTCAAGA TCAGCCAGCT 
AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGTCC CAGTGAATAT 
TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT 
TTTGCTGAAG ATACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG 
GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGATCAG 
TTGTCACCAC TGGAAAACAG TTCTACAAGT CCTCAGAGTG CAAGCACAAA TACAGTATTC 
CAGCCCACTC AAATGCAAGA ACCTCCTATA GCCACCGTCA CTACCACAGC CACCAGTGAT 
GAATTGAAAA CAGTGACAAA AGATGGTATG GAAGACATTA AAATACTGAT TGCATTTCCA 
TCTCCTCCCC ACGTACCTAA AGAACCTCCT TGTGCCACAA CGTCACCATA TAGTGATACT 
GGAAGTCGGA CAGCCTCACC CAACAGAGCA GGAAAAGGAG TCATAGAACA GACAGAAAAA 
TCTCATCCAA GAAGCCCTAA TGTGTTATCT GTCGCTTTGA GTCAAAGAAC TACTGCTCCT 
GAGGAAGAAC TAAATCCAAA GATACTAGCT TTGCAGAATG CTCAGAGAAA GCGAAAAATT 
GAACATGATG GTTCACTTTT TCAAGCAGTA GGAATTGGAA CATTATTACA GCAACCAGAT 
GATCGTGCAA CTACCACATC ACTTTCTTGG AAACGTGTAA AAGGATGCAA ATCTAGTGAA 
CAAAATGGAA TGGAGCAAAA GACAATTATT TTAATACCCT CTGATTTAGC ATGTAGACTG 
CTGGGGCAAT CAATGGATGA GAGTGGACTA CCACAGCTGA CCAGTTATGA TTGTGAAGTT 
AATGCTCCTA TACAAGGCAG CAGAAACCTA CTGCAGGGTG AAGAATTACT CAGAGCTTTG 
GATCAAGTTA ACTGA