TSHR cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSHR
Entrez Gene ID	281553
Full Name	thyroid stimulating hormone receptor
General protein information	Preferred Names thyroid stimulating hormone receptor Names thyrotropin receptor TSH-R
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005212136.4	XP_005212193.1	thyrotropin receptor isoform X1
NM_174206.3	NP_776631.1	thyrotropin receptor precursor
XM_005212136.3	XP_005212193.1	thyrotropin receptor isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSHR	NP_000360.2
Mus musculus (house mouse)	Tshr	NP_035778.3
Gallus gallus (chicken)	TSHR	NP_001180517.1
	TSHR	XP_001104839.1
Canis lupus familiaris (dog)	TSHR	NP_001003285.1
Bos taurus (cattle)	TSHR	NP_776631.1
Rattus norvegicus (Norway rat)	Tshr	NP_037020.1
Danio rerio (zebrafish)	tshr	NP_001139235.1
Pan troglodytes (chimpanzee)	TSHR	XP_001166989.1
Xenopus tropicalis (tropical clawed frog)	tshr	XP_002938419.2

Gene Ontology
Bibliography

Related articles in PubMed

Localization of thyrotropin receptor and thyroglobulin in the bovine corpus luteum.
Mutinati M, Desantis S, Rizzo A, Zizza S, Ventriglia G, Pantaleo M, Sciorsci RL
Animal reproduction science118(1)1-6(2010 Mar)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Extended hormone binding site of the human thyroid stimulating hormone receptor: distinctive acidic residues in the hinge region are involved in bovine thyroid stimulating hormone binding and receptor activation.
Mueller S, Kleinau G, Jaeschke H, Paschke R, Krause G
The Journal of biological chemistry283(26)18048-55(2008 Jun)

Increased receptor binding by bovine (b) TSH bound to monoclonal antibody to bTSHbeta-subunit.
Ochi Y, Hamazu M, Kajita Y, Hachiya T, Kunimatsu M, Sato T, Nagata A
Endocrine journal55(1)105-11(2008 Mar)

Ras homolog enriched in striatum inhibits the functional activity of wild type thyrotropin, follicle-stimulating hormone, luteinizing hormone receptors and activating thyrotropin receptor mutations by altering their expression in COS-7 cells.
Agretti P, De Marco G, Pinchera A, Vitti P, Bernal J, Tonacchera M
Journal of endocrinological investigation30(4)279-84(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Localization of thyrotropin receptor and thyroglobulin in the bovine corpus luteum.
Title: Localization of thyrotropin receptor and thyroglobulin in the bovine corpus luteum.

the hinge region represents an extracellular intermediate connector for both hormone binding and signal transduction of the thyroid stimulating hormone receptor
Title: Extended hormone binding site of the human thyroid stimulating hormone receptor: distinctive acidic residues in the hinge region are involved in bovine thyroid stimulating hormone binding and receptor activation.

Increased receptor binding by bovine (b) TSH bound to monoclonal antibody to bTSHbeta-subunit.
Title: Increased receptor binding by bovine (b) TSH bound to monoclonal antibody to bTSHbeta-subunit.

Rhes can interfere with the functional activity of wt and mutated TSHr.
Title: Ras homolog enriched in striatum inhibits the functional activity of wild type thyrotropin, follicle-stimulating hormone, luteinizing hormone receptors and activating thyrotropin receptor mutations by altering their expression in COS-7 cells.

analysis of activation switch in the thyrotropin receptor
Title: An activation switch in the rhodopsin family of G protein-coupled receptors: the thyrotropin receptor.

The following TSHR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSHR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa23577	XM_005212136.4 Latest version!	Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa01059	NM_174206.3 Latest version!	Bos taurus thyroid stimulating hormone receptor (TSHR), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OBa23577	XM_005212136.3	Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSHR ( XM_005212136.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212193.1 Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23577
Clone ID Related Accession (Same CDS sequence)	XM_005212136.4 , XM_005212136.3
Accession Version	XM_005212136.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	thyrotropin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212136.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTAAAAAAGT TCAGAAGAAA GTCCTTGTTC 
TTTGAGACTC AGAGGGTCCC ACACTCCACT GTGCCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212193.1
CDS	447..1208
Translation	MRPTPLLRLALFLVLPSSLGGERCPSPPCECRQEDDFRVTCKDIQSIPSLPPSTQTLKFIETHLKTIPSRAFSNLPNISRIYLSIDATLQQLESHSFYNLSKVTHIEIRNTRSLTYIDSGALKELPLLKFLGIFNTGLRVFPDLTKIYSTDVFFILEITDNPYMTSIPANAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTVIGQDAFAGVYSGPTLLKKFRRKSLFFETQRVPHSTVPS

Target ORF information:

RefSeq Version	XM_005212136.4
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212136.4

ORF Insert Sequence:

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTAAAAAAGT TCAGAAGAAA GTCCTTGTTC 
TTTGAGACTC AGAGGGTCCC ACACTCCACT GTGCCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHR ( XM_005212136.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005212193.1 Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23577
Clone ID Related Accession (Same CDS sequence)	XM_005212136.4 , XM_005212136.3
Accession Version	XM_005212136.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	thyrotropin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTAAAAAAGT TCAGAAGAAA GTCCTTGTTC 
TTTGAGACTC AGAGGGTCCC ACACTCCACT GTGCCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005212193.1
CDS	114..875
Translation	MRPTPLLRLALFLVLPSSLGGERCPSPPCECRQEDDFRVTCKDIQSIPSLPPSTQTLKFIETHLKTIPSRAFSNLPNISRIYLSIDATLQQLESHSFYNLSKVTHIEIRNTRSLTYIDSGALKELPLLKFLGIFNTGLRVFPDLTKIYSTDVFFILEITDNPYMTSIPANAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTVIGQDAFAGVYSGPTLLKKFRRKSLFFETQRVPHSTVPS

Target ORF information:

RefSeq Version	XM_005212136.3
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid stimulating hormone receptor (TSHR), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005212136.3

ORF Insert Sequence:

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTAAAAAAGT TCAGAAGAAA GTCCTTGTTC 
TTTGAGACTC AGAGGGTCCC ACACTCCACT GTGCCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSHR ( NM_174206.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776631.1 Bos taurus thyroid stimulating hormone receptor (TSHR), mRNA.

Price & Availability↑

CloneID	OBa01059
Clone ID Related Accession (Same CDS sequence)	NM_174206.3
Accession Version	NM_174206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Bos taurus(cattle)
Product	thyrotropin receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U15570.1. On Aug 31, 2012 this sequence version replaced NM_174206.2. ##Evidence-Data-START## Transcript exon combination :: U15570.1, U15568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145480 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTGGACATCT CTTATACCAG TGTCACAGCC 
CTACCATCCA AAGGCCTGGA ACACCTGAAG GAATTGATAG CAAGAAACAC TTGGACTCTA 
AGGAAACTTC CTCTTTCCTT GAGTTTCCTT CACCTCACAC GGGCTGACCT TTCTTATCCG 
AGCCACTGCT GCGCTTTTAA GAATCAGAAG AAAATCAGAG GAATCCTTCA GTCTTTAATG 
TGTAACGAGA GCAGTATTCG GGGCCTGCGT CAGAGAAAAT CCGCAAGTGC TTTGAATGGT 
CCCTTCTACC AGGAATATGA GGATCTGGGT GATGGCAGTG CTGGGTACAA GGAGAACTCC 
AAGTTCCAAG ATACCCAAAG CAACTCTCAT TACTATGTCT TCTTTGAGGA GCAAGAAGAT 
GAGATCATCG GTTTTGGCCA ACAGCTCAAA AACCCCCAGG AGGAGACCCT GCAGGCCTTT 
GACAGCCATT ACGACTATAC CGTGTGTGGG GGCAGTGAGG ACATGGTGTG TACCCCCAAG 
TCGGATGAGT TCAACCCCTG TGAGGACATC ATGGGCTACA AGTTCCTGAG AATCGTGGTG 
TGGTTTGTGA GTCTGCTGGC TCTCCTGGGC AACGTCTTCG TCCTGGTCAT CCTCCTCACG 
AGCCACTACA AGCTGACCGT CCCACGCTTC CTCATGTGCA ACCTGGCCTT TGCAGACTTC 
TGCATGGGGT TGTATCTGCT CCTCATCGCC TCCGTAGACC TCTACACTCA GTCTGAGTAC 
TACAACCATG CCATTGACTG GCAGACAGGC CCTGGCTGCA ACACAGCTGG CTTCTTCACC 
GTCTTTGCCA GCGAGCTGTC TGTGTACACG CTGACGGTCA TCACCTTGGA GCGCTGGCAC 
GCCATCACCT TCGCCATGCG CCTGGACCGC AAGATCCGCC TCTGGCACGC CTATGTCATC 
ATGCTGGGGG GCTGGGTTTG CTGCTTCCTG CTCGCCCTGC TCCCTTTGGT GGGAATAAGC 
AGCTATGCCA AGGTCAGCAT CTGCCTGCCC ATGGACACCG AGACTCCTCT TGCCCTGGCG 
TACATTATCC TTGTTCTGTT ACTCAACATC ATTGCCTTTA TCATCGTCTG TGCCTGTTAC 
GTGAAGATCT ACATCACAGT CCGAAATCCC CACTACAACC CGGGGGACAA AGATACCAGA 
ATTGCCAAAA GGATGGCTGT GTTGATCTTC ACTGACTTCA TGTGCATGGC CCCAATCTCT 
TTCTACGCTC TGTCGGCCCT TATGAACAAG CCTCTCATCA CCGTTACCAA TTCCAAAATC 
TTGCTGGTCC TCTTCTACCC ACTTAACTCC TGTGCCAATC CATTCCTCTA TGCCATTTTC 
ACCAAAGCCT TCCAGAGGGA TGTGTTTATG CTGCTCAGCA AGTTTGGCAT CTGTAAACGC 
CAGGCTCAGG CATACCGGGG TCAGAGAGTT TCTCCAAAGA ACAGCACTGG TATTCGGGTC 
CAAAAGGTTC CCCCAGATGT GAGGCAGAGT CTCCCCAATG TGCAGGATGA CTATGAGCTG 
CTTGAAAACT CTCATCTAAC CCCAAAGCAG CAGGACCAAA CCTCAAAAGA GTATAAGCGA 
ACAGTTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776631.1
CDS	61..2352
Translation	MRPTPLLRLALFLVLPSSLGGERCPSPPCECRQEDDFRVTCKDIQSIPSLPPSTQTLKFIETHLKTIPSRAFSNLPNISRIYLSIDATLQQLESHSFYNLSKVTHIEIRNTRSLTYIDSGALKELPLLKFLGIFNTGLRVFPDLTKIYSTDVFFILEITDNPYMTSIPANAFQGLCNETLTLKLYNNGFTSIQGHAFNGTKLDAVYLNKNKYLTVIGQDAFAGVYSGPTLLDISYTSVTALPSKGLEHLKELIARNTWTLRKLPLSLSFLHLTRADLSYPSHCCAFKNQKKIRGILQSLMCNESSIRGLRQRKSASALNGPFYQEYEDLGDGSAGYKENSKFQDTQSNSHYYVFFEEQEDEIIGFGQQLKNPQEETLQAFDSHYDYTVCGGSEDMVCTPKSDEFNPCEDIMGYKFLRIVVWFVSLLALLGNVFVLVILLTSHYKLTVPRFLMCNLAFADFCMGLYLLLIASVDLYTQSEYYNHAIDWQTGPGCNTAGFFTVFASELSVYTLTVITLERWHAITFAMRLDRKIRLWHAYVIMLGGWVCCFLLALLPLVGISSYAKVSICLPMDTETPLALAYIILVLLLNIIAFIIVCACYVKIYITVRNPHYNPGDKDTRIAKRMAVLIFTDFMCMAPISFYALSALMNKPLITVTNSKILLVLFYPLNSCANPFLYAIFTKAFQRDVFMLLSKFGICKRQAQAYRGQRVSPKNSTGIRVQKVPPDVRQSLPNVQDDYELLENSHLTPKQQDQTSKEYKRTVL

Target ORF information:

RefSeq Version	NM_174206.3
Organism	Bos taurus(cattle)
Definition	Bos taurus thyroid stimulating hormone receptor (TSHR), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174206.3

ORF Insert Sequence:

ATGCGGCCGA CGCCCCTCCT GCGGCTGGCG CTGTTTCTGG TCCTGCCCAG CAGCCTCGGT 
GGGGAGAGGT GTCCGTCTCC GCCCTGCGAA TGCCGCCAGG AGGACGACTT CAGAGTCACC 
TGCAAGGACA TCCAGAGCAT CCCTAGCTTA CCCCCCAGCA CGCAGACCCT GAAGTTTATA 
GAGACTCATC TGAAAACCAT TCCCAGTCGT GCGTTCTCAA ATCTGCCCAA TATTTCCAGG 
ATCTACTTGT CAATAGATGC AACTCTGCAG CAGCTGGAAT CACATTCCTT CTACAATTTA 
AGTAAAGTGA CTCACATAGA GATTCGGAAT ACCAGAAGTT TAACTTACAT AGACTCTGGC 
GCCCTAAAAG AGCTCCCCCT TCTAAAGTTC CTTGGCATTT TCAACACTGG ACTTAGAGTA 
TTCCCCGACC TGACCAAAAT CTATTCCACC GATGTGTTCT TCATACTTGA AATTACAGAC 
AACCCTTACA TGACTTCAAT CCCTGCGAAT GCTTTTCAGG GCCTGTGCAA CGAAACCTTG 
ACACTGAAGC TATACAACAA TGGCTTTACT TCAATCCAAG GACATGCTTT CAACGGGACA 
AAGCTGGATG CTGTTTACCT GAACAAGAAT AAATACCTGA CAGTCATCGG CCAAGATGCA 
TTTGCAGGGG TCTATAGCGG ACCAACCTTG CTGGACATCT CTTATACCAG TGTCACAGCC 
CTACCATCCA AAGGCCTGGA ACACCTGAAG GAATTGATAG CAAGAAACAC TTGGACTCTA 
AGGAAACTTC CTCTTTCCTT GAGTTTCCTT CACCTCACAC GGGCTGACCT TTCTTATCCG 
AGCCACTGCT GCGCTTTTAA GAATCAGAAG AAAATCAGAG GAATCCTTCA GTCTTTAATG 
TGTAACGAGA GCAGTATTCG GGGCCTGCGT CAGAGAAAAT CCGCAAGTGC TTTGAATGGT 
CCCTTCTACC AGGAATATGA GGATCTGGGT GATGGCAGTG CTGGGTACAA GGAGAACTCC 
AAGTTCCAAG ATACCCAAAG CAACTCTCAT TACTATGTCT TCTTTGAGGA GCAAGAAGAT 
GAGATCATCG GTTTTGGCCA ACAGCTCAAA AACCCCCAGG AGGAGACCCT GCAGGCCTTT 
GACAGCCATT ACGACTATAC CGTGTGTGGG GGCAGTGAGG ACATGGTGTG TACCCCCAAG 
TCGGATGAGT TCAACCCCTG TGAGGACATC ATGGGCTACA AGTTCCTGAG AATCGTGGTG 
TGGTTTGTGA GTCTGCTGGC TCTCCTGGGC AACGTCTTCG TCCTGGTCAT CCTCCTCACG 
AGCCACTACA AGCTGACCGT CCCACGCTTC CTCATGTGCA ACCTGGCCTT TGCAGACTTC 
TGCATGGGGT TGTATCTGCT CCTCATCGCC TCCGTAGACC TCTACACTCA GTCTGAGTAC 
TACAACCATG CCATTGACTG GCAGACAGGC CCTGGCTGCA ACACAGCTGG CTTCTTCACC 
GTCTTTGCCA GCGAGCTGTC TGTGTACACG CTGACGGTCA TCACCTTGGA GCGCTGGCAC 
GCCATCACCT TCGCCATGCG CCTGGACCGC AAGATCCGCC TCTGGCACGC CTATGTCATC 
ATGCTGGGGG GCTGGGTTTG CTGCTTCCTG CTCGCCCTGC TCCCTTTGGT GGGAATAAGC 
AGCTATGCCA AGGTCAGCAT CTGCCTGCCC ATGGACACCG AGACTCCTCT TGCCCTGGCG 
TACATTATCC TTGTTCTGTT ACTCAACATC ATTGCCTTTA TCATCGTCTG TGCCTGTTAC 
GTGAAGATCT ACATCACAGT CCGAAATCCC CACTACAACC CGGGGGACAA AGATACCAGA 
ATTGCCAAAA GGATGGCTGT GTTGATCTTC ACTGACTTCA TGTGCATGGC CCCAATCTCT 
TTCTACGCTC TGTCGGCCCT TATGAACAAG CCTCTCATCA CCGTTACCAA TTCCAAAATC 
TTGCTGGTCC TCTTCTACCC ACTTAACTCC TGTGCCAATC CATTCCTCTA TGCCATTTTC 
ACCAAAGCCT TCCAGAGGGA TGTGTTTATG CTGCTCAGCA AGTTTGGCAT CTGTAAACGC 
CAGGCTCAGG CATACCGGGG TCAGAGAGTT TCTCCAAAGA ACAGCACTGG TATTCGGGTC 
CAAAAGGTTC CCCCAGATGT GAGGCAGAGT CTCCCCAATG TGCAGGATGA CTATGAGCTG 
CTTGAAAACT CTCATCTAAC CCCAAAGCAG CAGGACCAAA CCTCAAAAGA GTATAAGCGA 
ACAGTTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Localization of thyrotropin receptor and thyroglobulin in the bovine corpus luteum.
Animal reproduction science118(1)1-6(2010 Mar)
Mutinati M,Desantis S,Rizzo A,Zizza S,Ventriglia G,Pantaleo M,Sciorsci RL

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Extended hormone binding site of the human thyroid stimulating hormone receptor: distinctive acidic residues in the hinge region are involved in bovine thyroid stimulating hormone binding and receptor activation.
The Journal of biological chemistry283(26)18048-55(2008 Jun)
Mueller S,Kleinau G,Jaeschke H,Paschke R,Krause G

Increased receptor binding by bovine (b) TSH bound to monoclonal antibody to bTSHbeta-subunit.
Endocrine journal55(1)105-11(2008 Mar)
Ochi Y,Hamazu M,Kajita Y,Hachiya T,Kunimatsu M,Sato T,Nagata A

Ras homolog enriched in striatum inhibits the functional activity of wild type thyrotropin, follicle-stimulating hormone, luteinizing hormone receptors and activating thyrotropin receptor mutations by altering their expression in COS-7 cells.
Journal of endocrinological investigation30(4)279-84(2007 Apr)
Agretti P,De Marco G,Pinchera A,Vitti P,Bernal J,Tonacchera M