ZP2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZP2
Entrez Gene ID	280963
Full Name	zona pellucida glycoprotein 2
Synonyms	Zp-2
General protein information	Preferred Names zona pellucida glycoprotein 2 Names zona pellucida sperm-binding protein 2 zona pellucida glycoprotein 2 (sperm receptor) zona pellucida glycoprotein ZP2 zona pellucida protein A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010819279.1	XP_010817581.1	zona pellucida sperm-binding protein 2 isoform X2
XM_010819278.2	XP_010817580.1	zona pellucida sperm-binding protein 2 isoform X1
XM_024984639.1	XP_024840407.1	zona pellucida sperm-binding protein 2 isoform X1
NM_173973.2	NP_776398.1	zona pellucida sperm-binding protein 2 precursor

Pathways from BioSystems

Homologies

	ZP2	XP_001091147.2
Canis lupus familiaris (dog)	ZP2	NP_001003304.1
Gallus gallus (chicken)	ZP2	NP_001034187.1
Xenopus tropicalis (tropical clawed frog)	zp2	NP_988855.1
Pan troglodytes (chimpanzee)	ZP2	XP_510869.3
Mus musculus (house mouse)	Zp2	NP_035905.1
Homo sapiens (human)	ZP2	NP_003451.1
Bos taurus (cattle)	ZP2	NP_776398.1
Rattus norvegicus (Norway rat)	Zp2	NP_112412.1

Gene Ontology
Bibliography

Related articles in PubMed

Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
Burkart AD, Xiong B, Baibakov B, Jim?nez-Movilla M, Dean J
The Journal of cell biology197(1)37-44(2012 Apr)

Evolutionary patterns of two major reproduction candidate genes (Zp2 and Zp3) reveal no contribution to reproductive isolation between bovine species.
Chen S, Costa V, Beja-Pereira A
BMC evolutionary biology1124(2011 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida.
Ikeda K, Yonezawa N, Naoi K, Katsumata T, Hamano S, Nakano M
European journal of biochemistry269(17)4257-66(2002 Sep)

Molecular cloning of bovine zona pellucida glycoproteins ZPA and ZPB and analysis for sperm-binding component of the zona.
Yonezawa N, Fukui N, Kuno M, Shinoda M, Goko S, Mitsui S, Nakano M
European journal of biochemistry268(12)3587-94(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polymorphism of Zp2 and the Zp3 did not show any contribution to reproductive isolation between the bovine species.
Title: Evolutionary patterns of two major reproduction candidate genes (Zp2 and Zp3) reveal no contribution to reproductive isolation between bovine species.

The following ZP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa32941	XM_010819279.1 Latest version!	Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa41735	NM_173973.2 Latest version!	Bos taurus zona pellucida glycoprotein 2 (ZP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa45042	XM_010819278.2 Latest version!	Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa230141	XM_024984639.1 Latest version!	Bos taurus zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZP2 ( XM_010819279.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817581.1 Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa32941
Clone ID Related Accession (Same CDS sequence)	XM_010819279.1
Accession Version	XM_010819279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zona pellucida sperm-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGTGG AGTTTAAAAG AACTCTTGGC AACAAGATTC AGCATGCATC TGTGGTGGAT 
TCCCTTGGTC TTAAAATGCT GAACTGCACT TATGTTCTGG ACCCAGAAAA GCTCACACTG 
AAGGCCCCAT ATGAAAGCTG TACCAAAAGA GTGCTTGGCC AGCACCAGAT GACCATCACA 
TTCATGAATG ACAATACTGC CCATAGACAA AAGACTGTCT TGTATCACGT CAGCTGTCCG 
GTTATGCAAG CAGGAAGACA TGATCAGCAT TCGGGATCCA CAATCTGCTC GAAGGATTTC 
ATGTCTTTTA CCTTTCACTT TTTTCCTGGC CTGGCTGACG ACACTGCGGG TCCCAAACCT 
CAGATGGGAT GGACCGTTAC AGTTGGTGAT GGTGAAAGAG CCCAAAATCT GACCCTTCAA 
GAGGCTCTGA CACAAGGATA TAATCTCCTG ATTGAGAACC AGAAGATGAG CATCCAAGTG 
TTATTCCATG CCACTGGAGT GACTCACTAC TCGCAAGGTA ACAGCCATCT CTACATGGTG 
CCTCTGAAGC TTACGCATGT ATCTCCTGGG CAGACGATCA TCTTATCATC ACGACTCCTC 
TGTGCATCAG ATCCTGTGAC CTGTAATGCC ACACACATGA CTCTCACCAT ACCAGAATTT 
CCTGGGAAGT TAAAATCTGT GAGCTTTGAA AATAAGAATA TTGCTGTGAA CCAGTTGCAC 
AACAGCGGGA TTGTTATGGA AATAGCAAAT GGCCTCAGGT TGCATTTCAG CAAAACTCTC 
CTCAAAACAA AATTCTCTGA AAAATGCCTA CCCTATCAGT TCTACTTATC TTCACTCAAG 
CTGACGTTTT ATACTCAACT GGAGACAGTA TCCATGGTGA TTTATCCTGA ATGTGTCTGC 
GAGTCAACAG TTTCTATAGT TTCAGGGGAG CTATGTACTC AAGATGGGTT TATGGACGTC 
GAGGTCTACC ACCATCAAAC AAAACCAGCT CTCAACTTGG ACACTCTTAG GGTGGGAGAC 
TCCTCCTGCC AGCCAACCAT TAAAGCTCCA TTTCAAGGAC TGGTGAAGTT TCACATACCC 
CTGAATGGAT GTGGAACAAG ACATAAGTTT GAGAATGGCA AAGTCATCTA TGAAAATGAA 
ATACATGCTC TCTGGGCGGA TCTTCCTCCA AGCACAATTT CCAGAGATAG TGAGTTCAGA 
ATGACGGTGA GGTGCTATTA TAGCAGCAGT AACATGCTAA TAAATACCAA TGTCGAAAGC 
CTTCCTCCTC CAGTAGCCTC AGTGAAGCCA GGTCCACTTG CCTTGACCCT GCAAACATAC 
CCAGATAATT CCTACCTGCA ACCGTACGGG GACAAGGACT ATCCTGTGGT GAGATACCTC 
CGCCAACCAA TTTACTTAGA AGTGAGAGTC CTAAACAGGA CTGACCCCAA CATCAAGTTG 
GTCTTAGATG ATTGCTGGGC AACATCCACC ATGGACCCAG CCTCTCTCCC TCAGTGGAAT 
ATTATCGTGG ATGGCTGTGA ATACAACTTG GACAACCACA GAACCACCTT CCATCCGGTT 
GGCTCCTCGG TGGCCTATCC TAATCACTAC CAGAGGTTTG CTGTGAAGAC CTTTGCCTTT 
GTGTCAGAGG ACCCGGCGTT CTCTCACTTG GTCTACTTCC ACTGCAGCGC CTTAATCTGC 
GATCAACTTT CTTCTAACTT CCCCCTGTGT TCTGCGTCTT GCCTTGTGTC ATCCAGAAGC 
AGGCGAGCCA CAGGGGCCAC TGAGGAAGAG AAGATGATAG TGAGTCTCCC GGGCCCCATC 
CTCCTGTTGT CAGATGGCTC TTCATTCAGA GATGCTGTGG ATTCTAAAGG GCATGGGACT 
TCTGGATATG CTGCTTTTAA AACTATGGTT GCTGTAGTTG CCTTAGCAGG TGTTGTGGCA 
ACTCTAAGCC TAATCAGCTA CCTGCGCAAG AAAAGAATCA CAGTGCTAAA CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817581.1
CDS	260..2236
Translation	MVVEFKRTLGNKIQHASVVDSLGLKMLNCTYVLDPEKLTLKAPYESCTKRVLGQHQMTITFMNDNTAHRQKTVLYHVSCPVMQAGRHDQHSGSTICSKDFMSFTFHFFPGLADDTAGPKPQMGWTVTVGDGERAQNLTLQEALTQGYNLLIENQKMSIQVLFHATGVTHYSQGNSHLYMVPLKLTHVSPGQTIILSSRLLCASDPVTCNATHMTLTIPEFPGKLKSVSFENKNIAVNQLHNSGIVMEIANGLRLHFSKTLLKTKFSEKCLPYQFYLSSLKLTFYTQLETVSMVIYPECVCESTVSIVSGELCTQDGFMDVEVYHHQTKPALNLDTLRVGDSSCQPTIKAPFQGLVKFHIPLNGCGTRHKFENGKVIYENEIHALWADLPPSTISRDSEFRMTVRCYYSSSNMLINTNVESLPPPVASVKPGPLALTLQTYPDNSYLQPYGDKDYPVVRYLRQPIYLEVRVLNRTDPNIKLVLDDCWATSTMDPASLPQWNIIVDGCEYNLDNHRTTFHPVGSSVAYPNHYQRFAVKTFAFVSEDPAFSHLVYFHCSALICDQLSSNFPLCSASCLVSSRSRRATGATEEEKMIVSLPGPILLLSDGSSFRDAVDSKGHGTSGYAAFKTMVAVVALAGVVATLSLISYLRKKRITVLNH

Target ORF information:

RefSeq Version	XM_010819279.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819279.1

ORF Insert Sequence:

ATGGTAGTGG AGTTTAAAAG AACTCTTGGC AACAAGATTC AGCATGCATC TGTGGTGGAT 
TCCCTTGGTC TTAAAATGCT GAACTGCACT TATGTTCTGG ACCCAGAAAA GCTCACACTG 
AAGGCCCCAT ATGAAAGCTG TACCAAAAGA GTGCTTGGCC AGCACCAGAT GACCATCACA 
TTCATGAATG ACAATACTGC CCATAGACAA AAGACTGTCT TGTATCACGT CAGCTGTCCG 
GTTATGCAAG CAGGAAGACA TGATCAGCAT TCGGGATCCA CAATCTGCTC GAAGGATTTC 
ATGTCTTTTA CCTTTCACTT TTTTCCTGGC CTGGCTGACG ACACTGCGGG TCCCAAACCT 
CAGATGGGAT GGACCGTTAC AGTTGGTGAT GGTGAAAGAG CCCAAAATCT GACCCTTCAA 
GAGGCTCTGA CACAAGGATA TAATCTCCTG ATTGAGAACC AGAAGATGAG CATCCAAGTG 
TTATTCCATG CCACTGGAGT GACTCACTAC TCGCAAGGTA ACAGCCATCT CTACATGGTG 
CCTCTGAAGC TTACGCATGT ATCTCCTGGG CAGACGATCA TCTTATCATC ACGACTCCTC 
TGTGCATCAG ATCCTGTGAC CTGTAATGCC ACACACATGA CTCTCACCAT ACCAGAATTT 
CCTGGGAAGT TAAAATCTGT GAGCTTTGAA AATAAGAATA TTGCTGTGAA CCAGTTGCAC 
AACAGCGGGA TTGTTATGGA AATAGCAAAT GGCCTCAGGT TGCATTTCAG CAAAACTCTC 
CTCAAAACAA AATTCTCTGA AAAATGCCTA CCCTATCAGT TCTACTTATC TTCACTCAAG 
CTGACGTTTT ATACTCAACT GGAGACAGTA TCCATGGTGA TTTATCCTGA ATGTGTCTGC 
GAGTCAACAG TTTCTATAGT TTCAGGGGAG CTATGTACTC AAGATGGGTT TATGGACGTC 
GAGGTCTACC ACCATCAAAC AAAACCAGCT CTCAACTTGG ACACTCTTAG GGTGGGAGAC 
TCCTCCTGCC AGCCAACCAT TAAAGCTCCA TTTCAAGGAC TGGTGAAGTT TCACATACCC 
CTGAATGGAT GTGGAACAAG ACATAAGTTT GAGAATGGCA AAGTCATCTA TGAAAATGAA 
ATACATGCTC TCTGGGCGGA TCTTCCTCCA AGCACAATTT CCAGAGATAG TGAGTTCAGA 
ATGACGGTGA GGTGCTATTA TAGCAGCAGT AACATGCTAA TAAATACCAA TGTCGAAAGC 
CTTCCTCCTC CAGTAGCCTC AGTGAAGCCA GGTCCACTTG CCTTGACCCT GCAAACATAC 
CCAGATAATT CCTACCTGCA ACCGTACGGG GACAAGGACT ATCCTGTGGT GAGATACCTC 
CGCCAACCAA TTTACTTAGA AGTGAGAGTC CTAAACAGGA CTGACCCCAA CATCAAGTTG 
GTCTTAGATG ATTGCTGGGC AACATCCACC ATGGACCCAG CCTCTCTCCC TCAGTGGAAT 
ATTATCGTGG ATGGCTGTGA ATACAACTTG GACAACCACA GAACCACCTT CCATCCGGTT 
GGCTCCTCGG TGGCCTATCC TAATCACTAC CAGAGGTTTG CTGTGAAGAC CTTTGCCTTT 
GTGTCAGAGG ACCCGGCGTT CTCTCACTTG GTCTACTTCC ACTGCAGCGC CTTAATCTGC 
GATCAACTTT CTTCTAACTT CCCCCTGTGT TCTGCGTCTT GCCTTGTGTC ATCCAGAAGC 
AGGCGAGCCA CAGGGGCCAC TGAGGAAGAG AAGATGATAG TGAGTCTCCC GGGCCCCATC 
CTCCTGTTGT CAGATGGCTC TTCATTCAGA GATGCTGTGG ATTCTAAAGG GCATGGGACT 
TCTGGATATG CTGCTTTTAA AACTATGGTT GCTGTAGTTG CCTTAGCAGG TGTTGTGGCA 
ACTCTAAGCC TAATCAGCTA CCTGCGCAAG AAAAGAATCA CAGTGCTAAA CCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZP2 ( NM_173973.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776398.1 Bos taurus zona pellucida glycoprotein 2 (ZP2), mRNA.

Price & Availability↑

CloneID	OBa41735
Clone ID Related Accession (Same CDS sequence)	NM_173973.2
Accession Version	NM_173973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Bos taurus(cattle)
Product	zona pellucida sperm-binding protein 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB042653.1. On Jun 3, 2003 this sequence version replaced NM_173973.1. ##Evidence-Data-START## Transcript exon combination :: AB042653.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145431 [ECO:0000350] ##Evidence-Data-END##

ATGGCATGCA GGCAGAGAGG AGACAGCGGG AGACCCTCAA GCTGGTTCCG TGCAGACTGG 
AGGTCATTTT TTCTATCTTT CACCCTTTTG ACTTCGGTGA ACTCAATAGA TGTTAATCAG 
TTGGATCCTG CCTTCCCAGG TACTGTCACT TGCTATGAAA ATAGAATGGT AGTGGAGTTT 
AAAAGAACTC TTGGCAACAA GATTCAGCAT GCATCTGTGG TGGATTCCCT TGGTCTTAAA 
ATGCTGAACT GCACTTATGT TCTGGACCCA GAAAAGCTCA CACTGAAGGC CCCATATGAA 
AGCTGTACCA AAAGAGTGCT TGGCCAGCAC CAGATGACCA TCACATTCAT GAATGACAAT 
ACTGCCCATA GACAAAAGAC TGTCTTGTAT CACGTCAGCT GTCCGGTTAT GCAAGCAGGA 
AGACATGATC AGCATTCGGG ATCCACAATC TGCTCGAAGG ATTTCATGTC TTTTACCTTT 
CACTTTTTTC CTGGCCTGGC TGACGACACT GCGGGTCCCA AACCTCAGAT GGGATGGACC 
GTTACAGTTG GTGATGGTGA AAGAGCCCAA AATCTGACCC TTCAAGAGGC TCTGACACAA 
GGATATAATC TCCTGATTGA GAACCAGAAG ATGAGCATCC AAGTGTTATT CCATGCCACT 
GGAGTGACTC ACTACTCGCA AGGTAACAGC CATCTCTACA TGGTGCCTCT GAAGCTTACG 
CATGTATCTC CTGGGCAGAC GATCATCTTA TCATCACGAC TCATCTGTGC ATCAGATCCT 
GTGACCTGTA ATGCCACACA CATGACTCTC ACCATACCAG AATTTCCTGG GAAGTTAAAA 
TCTGTGAGCT TTGAAAATAA GAATATTGCT GTGAACCAGT TGCACAACAG CGGGATTGTT 
ATGGAAATAG CAAATGGCCT CAGGTTGCAT TTCAGCAAAA CTCTCCTCAA AACAAAATTC 
TCTGAAAAAT GCCTACCCTA TCAGTTCTAC TTATCTTCAC TCAAGCTGAC GTTTTATACT 
CAACTGGAGA CAGTATCCAT GGTGATTTAT CCTGAATGTG TCTGCGAGTC AACAGTTTCT 
ATAGTTTCAG GGGAGCTATG TACTCAAGAT GGGTTTATGG ATGTCGAGGT CTACCGCCAT 
CAAACAAAAC CAGCTCTCAA CTTGGACACT CTTAGGGTGG GAGACTCCTC CTGCCAGCCA 
ACCATTAAAG CTCCATTTCA AGGACTGGTG AAGTTTCACA TACCCCTGAA TGGATGTGGA 
ACAAGACATA AGTTTGAGAA TGGCAAAGTC ATCTATGAAA ATGAAATACA TGCTCTCTGG 
GCGGATCTTC CTCCAAGCAC AATTTCCAGA GATAGTGAGT TCAGAATGAC GGTGAGGTGC 
TATTATAGCA GCAGTAACAT GCTAATAAAT ACCAATGTCG AAAGCCTTCC TCCTCCAGTA 
GCCTCAGTGA AGCCAGGTCC ACTTGCCTTG ACCCTGCAAA CCTACCCAGA TAATTCCTAC 
CTGCAACCGT ACGGGGACAA GGACTATCCT GTGGTGAGAT ACCTCCGCCA ACCAATTTAC 
TTAGAAGTGA GAGTCCTAAA CAGGACTGAC CCCAACATCA AGTTGGTCTT AGATGATTGC 
TGGGCAACAT CCACCATGGA CCCAGCCTCT CTCCCTCAGT GGAATATTAT CGTGGATGGC 
TGTGAATACA ACTTGGACAA CCACAGAACC ACCTTCCATC CGGTTGGCTC CTCGGTGGCC 
TATCCTAATC ACTACCAGAG GTTTGCTGTG AAGACCTTTG CCTTTGTGTC AGAGGACCCG 
GCATTCTCTC ACTTGGTCTA CTTCCACTGC AGCGCCTTAA TCTGCGATCA ACTTTCTTCT 
AACTTCCCCC TGTGTTCTGC GTCTTGCCTT GTGTCATCCA GAAGCAGGCG AGCCACAGGG 
GCCACTGAGG AAGAGAAGAT GATAGTGAGT CTCCCGGGCC CCATCCTCCT GTTGTCAGAT 
GGCTCTTCAT TCAGAGATGC TGTGGATTCT AAAGGGCATG GGACTTCTGG ATATGCTGCT 
TTTAAAACTA TGGTTGCTGT AGTTGCCTTA GCAGGTGTTG TGGCAACTCT AAGCCTAATC 
AGCTACCTGC GCAAGAAAAG AATCACAGTG CTAAACCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776398.1
CDS	30..2171
Translation	MACRQRGDSGRPSSWFRADWRSFFLSFTLLTSVNSIDVNQLDPAFPGTVTCYENRMVVEFKRTLGNKIQHASVVDSLGLKMLNCTYVLDPEKLTLKAPYESCTKRVLGQHQMTITFMNDNTAHRQKTVLYHVSCPVMQAGRHDQHSGSTICSKDFMSFTFHFFPGLADDTAGPKPQMGWTVTVGDGERAQNLTLQEALTQGYNLLIENQKMSIQVLFHATGVTHYSQGNSHLYMVPLKLTHVSPGQTIILSSRLICASDPVTCNATHMTLTIPEFPGKLKSVSFENKNIAVNQLHNSGIVMEIANGLRLHFSKTLLKTKFSEKCLPYQFYLSSLKLTFYTQLETVSMVIYPECVCESTVSIVSGELCTQDGFMDVEVYRHQTKPALNLDTLRVGDSSCQPTIKAPFQGLVKFHIPLNGCGTRHKFENGKVIYENEIHALWADLPPSTISRDSEFRMTVRCYYSSSNMLINTNVESLPPPVASVKPGPLALTLQTYPDNSYLQPYGDKDYPVVRYLRQPIYLEVRVLNRTDPNIKLVLDDCWATSTMDPASLPQWNIIVDGCEYNLDNHRTTFHPVGSSVAYPNHYQRFAVKTFAFVSEDPAFSHLVYFHCSALICDQLSSNFPLCSASCLVSSRSRRATGATEEEKMIVSLPGPILLLSDGSSFRDAVDSKGHGTSGYAAFKTMVAVVALAGVVATLSLISYLRKKRITVLNH

Target ORF information:

RefSeq Version	NM_173973.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zona pellucida glycoprotein 2 (ZP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173973.2

ORF Insert Sequence:

ATGGCATGCA GGCAGAGAGG AGACAGCGGG AGACCCTCAA GCTGGTTCCG TGCAGACTGG 
AGGTCATTTT TTCTATCTTT CACCCTTTTG ACTTCGGTGA ACTCAATAGA TGTTAATCAG 
TTGGATCCTG CCTTCCCAGG TACTGTCACT TGCTATGAAA ATAGAATGGT AGTGGAGTTT 
AAAAGAACTC TTGGCAACAA GATTCAGCAT GCATCTGTGG TGGATTCCCT TGGTCTTAAA 
ATGCTGAACT GCACTTATGT TCTGGACCCA GAAAAGCTCA CACTGAAGGC CCCATATGAA 
AGCTGTACCA AAAGAGTGCT TGGCCAGCAC CAGATGACCA TCACATTCAT GAATGACAAT 
ACTGCCCATA GACAAAAGAC TGTCTTGTAT CACGTCAGCT GTCCGGTTAT GCAAGCAGGA 
AGACATGATC AGCATTCGGG ATCCACAATC TGCTCGAAGG ATTTCATGTC TTTTACCTTT 
CACTTTTTTC CTGGCCTGGC TGACGACACT GCGGGTCCCA AACCTCAGAT GGGATGGACC 
GTTACAGTTG GTGATGGTGA AAGAGCCCAA AATCTGACCC TTCAAGAGGC TCTGACACAA 
GGATATAATC TCCTGATTGA GAACCAGAAG ATGAGCATCC AAGTGTTATT CCATGCCACT 
GGAGTGACTC ACTACTCGCA AGGTAACAGC CATCTCTACA TGGTGCCTCT GAAGCTTACG 
CATGTATCTC CTGGGCAGAC GATCATCTTA TCATCACGAC TCATCTGTGC ATCAGATCCT 
GTGACCTGTA ATGCCACACA CATGACTCTC ACCATACCAG AATTTCCTGG GAAGTTAAAA 
TCTGTGAGCT TTGAAAATAA GAATATTGCT GTGAACCAGT TGCACAACAG CGGGATTGTT 
ATGGAAATAG CAAATGGCCT CAGGTTGCAT TTCAGCAAAA CTCTCCTCAA AACAAAATTC 
TCTGAAAAAT GCCTACCCTA TCAGTTCTAC TTATCTTCAC TCAAGCTGAC GTTTTATACT 
CAACTGGAGA CAGTATCCAT GGTGATTTAT CCTGAATGTG TCTGCGAGTC AACAGTTTCT 
ATAGTTTCAG GGGAGCTATG TACTCAAGAT GGGTTTATGG ATGTCGAGGT CTACCGCCAT 
CAAACAAAAC CAGCTCTCAA CTTGGACACT CTTAGGGTGG GAGACTCCTC CTGCCAGCCA 
ACCATTAAAG CTCCATTTCA AGGACTGGTG AAGTTTCACA TACCCCTGAA TGGATGTGGA 
ACAAGACATA AGTTTGAGAA TGGCAAAGTC ATCTATGAAA ATGAAATACA TGCTCTCTGG 
GCGGATCTTC CTCCAAGCAC AATTTCCAGA GATAGTGAGT TCAGAATGAC GGTGAGGTGC 
TATTATAGCA GCAGTAACAT GCTAATAAAT ACCAATGTCG AAAGCCTTCC TCCTCCAGTA 
GCCTCAGTGA AGCCAGGTCC ACTTGCCTTG ACCCTGCAAA CCTACCCAGA TAATTCCTAC 
CTGCAACCGT ACGGGGACAA GGACTATCCT GTGGTGAGAT ACCTCCGCCA ACCAATTTAC 
TTAGAAGTGA GAGTCCTAAA CAGGACTGAC CCCAACATCA AGTTGGTCTT AGATGATTGC 
TGGGCAACAT CCACCATGGA CCCAGCCTCT CTCCCTCAGT GGAATATTAT CGTGGATGGC 
TGTGAATACA ACTTGGACAA CCACAGAACC ACCTTCCATC CGGTTGGCTC CTCGGTGGCC 
TATCCTAATC ACTACCAGAG GTTTGCTGTG AAGACCTTTG CCTTTGTGTC AGAGGACCCG 
GCATTCTCTC ACTTGGTCTA CTTCCACTGC AGCGCCTTAA TCTGCGATCA ACTTTCTTCT 
AACTTCCCCC TGTGTTCTGC GTCTTGCCTT GTGTCATCCA GAAGCAGGCG AGCCACAGGG 
GCCACTGAGG AAGAGAAGAT GATAGTGAGT CTCCCGGGCC CCATCCTCCT GTTGTCAGAT 
GGCTCTTCAT TCAGAGATGC TGTGGATTCT AAAGGGCATG GGACTTCTGG ATATGCTGCT 
TTTAAAACTA TGGTTGCTGT AGTTGCCTTA GCAGGTGTTG TGGCAACTCT AAGCCTAATC 
AGCTACCTGC GCAAGAAAAG AATCACAGTG CTAAACCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZP2 ( XM_010819278.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817580.1 Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45042
Clone ID Related Accession (Same CDS sequence)	XM_010819278.2
Accession Version	XM_010819278.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zona pellucida sperm-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819278.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATGCA GGCAGAGAGG AGACAGCGGG AGACCCTCAA GCTGGTTCCG TGCAGACTGG 
AGGTCATTTT TTCTATCTTT CACCCTTTTG ACTTCGGTGA ACTCAATAGA TGTTAATCAG 
TTGGATCCTG CCTTCCCAGG TACTGTCACT TGCTATGAAA ATAGAATGGT AGTGGAGTTT 
AAAAGAACTC TTGGCAACAA GATTCAGCAT GCATCTGTGG TGGATTCCCT TGGTCTTAAA 
ATGCTGAACT GCACTTATGT TCTGGACCCA GAAAAGCTCA CACTGAAGGC CCCATATGAA 
AGCTGTACCA AAAGAGTGCT TGGCCAGCAC CAGATGACCA TCACATTCAT GAATGACAAT 
ACTGCCCATA GACAAAAGAC TGTCTTGTAT CACGTCAGCT GTCCGGTTAT GCAAGCAGGA 
AGACATGATC AGCATTCGGG ATCCACAATC TGCTCGAAGG ATTTCATGTC TGGTCCCAAA 
CCTCAGATGG GATGGACCGT TACAGTTGGT GATGGTGAAA GAGCCCAAAA TCTGACCCTT 
CAAGAGGCTC TGACACAAGG ATATAATCTC CTGATTGAGA ACCAGAAGAT GAGCATCCAA 
GTGTTATTCC ATGCCACTGG AGTGACTCAC TACTCGCAAG GTAACAGCCA TCTCTACATG 
GTGCCTCTGA AGCTTACGCA TGTATCTCCT GGGCAGACGA TCATCTTATC ATCACGACTC 
CTCTGTGCAT CAGATCCTGT GACCTGTAAT GCCACACACA TGACTCTCAC CATACCAGAA 
TTTCCTGGGA AGTTAAAATC TGTGAGCTTT GAAAATAAGA ATATTGCTGT GAACCAGTTG 
CACAACAGCG GGATTGTTAT GGAAATAGCA AATGGCCTCA GGTTGCATTT CAGCAAAACT 
CTCCTCAAAA CAAAATTCTC TGAAAAATGC CTACCCTATC AGTTCTACTT ATCTTCACTC 
AAGCTGACGT TTTATACTCA ACTGGAGACA GTATCCATGG TGATTTATCC TGAATGTGTC 
TGCGAGTCAA CAGTTTCTAT AGTTTCAGGG GAGCTATGTA CTCAAGATGG GTTTATGGAC 
GTCGAGGTCT ACCACCATCA AACAAAACCA GCTCTCAACT TGGACACTCT TAGGGTGGGA 
GACTCCTCCT GCCAGCCAAC CATTAAAGCT CCATTTCAAG GACTGGTGAA GTTTCACATA 
CCCCTGAATG GATGTGGAAC AAGACATAAG TTTGAGAATG GCAAAGTCAT CTATGAAAAT 
GAAATACATG CTCTCTGGGC GGATCTTCCT CCAAGCACAA TTTCCAGAGA TAGTGAGTTC 
AGAATGACGG TGAGGTGCTA TTATAGCAGC AGTAACATGC TAATAAATAC CAATGTCGAA 
AGCCTTCCTC CTCCAGTAGC CTCAGTGAAG CCAGGTCCAC TTGCCTTGAC CCTGCAAACA 
TACCCAGATA ATTCCTACCT GCAACCGTAC GGGGACAAGG ACTATCCTGT GGTGAGATAC 
CTCCGCCAAC CAATTTACTT AGAAGTGAGA GTCCTAAACA GGACTGACCC CAACATCAAG 
TTGGTCTTAG ATGATTGCTG GGCAACATCC ACCATGGACC CAGCCTCTCT CCCTCAGTGG 
AATATTATCG TGGATGGCTG TGAATACAAC TTGGACAACC ACAGAACCAC CTTCCATCCG 
GTTGGCTCCT CGGTGGCCTA TCCTAATCAC TACCAGAGGT TTGCTGTGAA GACCTTTGCC 
TTTGTGTCAG AGGACCCGGC GTTCTCTCAC TTGGTCTACT TCCACTGCAG CGCCTTAATC 
TGCGATCAAC TTTCTTCTAA CTTCCCCCTG TGTTCTGCGT CTTGCCTTGT GTCATCCAGA 
AGCAGGCGAG CCACAGGGGC CACTGAGGAA GAGAAGATGA TAGTGAGTCT CCCGGGCCCC 
ATCCTCCTGT TGTCAGATGG CTCTTCATTC AGAGATGCTG TGGATTCTAA AGGGCATGGG 
ACTTCTGGAT ATGCTGCTTT TAAAACTATG GTTGCTGTAG TTGCCTTAGC AGGTGTTGTG 
GCAACTCTAA GCCTAATCAG CTACCTGCGC AAGAAAAGAA TCACAGTGCT AAACCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817580.1
CDS	27..2126
Translation	MACRQRGDSGRPSSWFRADWRSFFLSFTLLTSVNSIDVNQLDPAFPGTVTCYENRMVVEFKRTLGNKIQHASVVDSLGLKMLNCTYVLDPEKLTLKAPYESCTKRVLGQHQMTITFMNDNTAHRQKTVLYHVSCPVMQAGRHDQHSGSTICSKDFMSGPKPQMGWTVTVGDGERAQNLTLQEALTQGYNLLIENQKMSIQVLFHATGVTHYSQGNSHLYMVPLKLTHVSPGQTIILSSRLLCASDPVTCNATHMTLTIPEFPGKLKSVSFENKNIAVNQLHNSGIVMEIANGLRLHFSKTLLKTKFSEKCLPYQFYLSSLKLTFYTQLETVSMVIYPECVCESTVSIVSGELCTQDGFMDVEVYHHQTKPALNLDTLRVGDSSCQPTIKAPFQGLVKFHIPLNGCGTRHKFENGKVIYENEIHALWADLPPSTISRDSEFRMTVRCYYSSSNMLINTNVESLPPPVASVKPGPLALTLQTYPDNSYLQPYGDKDYPVVRYLRQPIYLEVRVLNRTDPNIKLVLDDCWATSTMDPASLPQWNIIVDGCEYNLDNHRTTFHPVGSSVAYPNHYQRFAVKTFAFVSEDPAFSHLVYFHCSALICDQLSSNFPLCSASCLVSSRSRRATGATEEEKMIVSLPGPILLLSDGSSFRDAVDSKGHGTSGYAAFKTMVAVVALAGVVATLSLISYLRKKRITVLNH

Target ORF information:

RefSeq Version	XM_010819278.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zona pellucida glycoprotein 2 (sperm receptor) (ZP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010819278.2

ORF Insert Sequence:

ATGGCATGCA GGCAGAGAGG AGACAGCGGG AGACCCTCAA GCTGGTTCCG TGCAGACTGG 
AGGTCATTTT TTCTATCTTT CACCCTTTTG ACTTCGGTGA ACTCAATAGA TGTTAATCAG 
TTGGATCCTG CCTTCCCAGG TACTGTCACT TGCTATGAAA ATAGAATGGT AGTGGAGTTT 
AAAAGAACTC TTGGCAACAA GATTCAGCAT GCATCTGTGG TGGATTCCCT TGGTCTTAAA 
ATGCTGAACT GCACTTATGT TCTGGACCCA GAAAAGCTCA CACTGAAGGC CCCATATGAA 
AGCTGTACCA AAAGAGTGCT TGGCCAGCAC CAGATGACCA TCACATTCAT GAATGACAAT 
ACTGCCCATA GACAAAAGAC TGTCTTGTAT CACGTCAGCT GTCCGGTTAT GCAAGCAGGA 
AGACATGATC AGCATTCGGG ATCCACAATC TGCTCGAAGG ATTTCATGTC TGGTCCCAAA 
CCTCAGATGG GATGGACCGT TACAGTTGGT GATGGTGAAA GAGCCCAAAA TCTGACCCTT 
CAAGAGGCTC TGACACAAGG ATATAATCTC CTGATTGAGA ACCAGAAGAT GAGCATCCAA 
GTGTTATTCC ATGCCACTGG AGTGACTCAC TACTCGCAAG GTAACAGCCA TCTCTACATG 
GTGCCTCTGA AGCTTACGCA TGTATCTCCT GGGCAGACGA TCATCTTATC ATCACGACTC 
CTCTGTGCAT CAGATCCTGT GACCTGTAAT GCCACACACA TGACTCTCAC CATACCAGAA 
TTTCCTGGGA AGTTAAAATC TGTGAGCTTT GAAAATAAGA ATATTGCTGT GAACCAGTTG 
CACAACAGCG GGATTGTTAT GGAAATAGCA AATGGCCTCA GGTTGCATTT CAGCAAAACT 
CTCCTCAAAA CAAAATTCTC TGAAAAATGC CTACCCTATC AGTTCTACTT ATCTTCACTC 
AAGCTGACGT TTTATACTCA ACTGGAGACA GTATCCATGG TGATTTATCC TGAATGTGTC 
TGCGAGTCAA CAGTTTCTAT AGTTTCAGGG GAGCTATGTA CTCAAGATGG GTTTATGGAC 
GTCGAGGTCT ACCACCATCA AACAAAACCA GCTCTCAACT TGGACACTCT TAGGGTGGGA 
GACTCCTCCT GCCAGCCAAC CATTAAAGCT CCATTTCAAG GACTGGTGAA GTTTCACATA 
CCCCTGAATG GATGTGGAAC AAGACATAAG TTTGAGAATG GCAAAGTCAT CTATGAAAAT 
GAAATACATG CTCTCTGGGC GGATCTTCCT CCAAGCACAA TTTCCAGAGA TAGTGAGTTC 
AGAATGACGG TGAGGTGCTA TTATAGCAGC AGTAACATGC TAATAAATAC CAATGTCGAA 
AGCCTTCCTC CTCCAGTAGC CTCAGTGAAG CCAGGTCCAC TTGCCTTGAC CCTGCAAACA 
TACCCAGATA ATTCCTACCT GCAACCGTAC GGGGACAAGG ACTATCCTGT GGTGAGATAC 
CTCCGCCAAC CAATTTACTT AGAAGTGAGA GTCCTAAACA GGACTGACCC CAACATCAAG 
TTGGTCTTAG ATGATTGCTG GGCAACATCC ACCATGGACC CAGCCTCTCT CCCTCAGTGG 
AATATTATCG TGGATGGCTG TGAATACAAC TTGGACAACC ACAGAACCAC CTTCCATCCG 
GTTGGCTCCT CGGTGGCCTA TCCTAATCAC TACCAGAGGT TTGCTGTGAA GACCTTTGCC 
TTTGTGTCAG AGGACCCGGC GTTCTCTCAC TTGGTCTACT TCCACTGCAG CGCCTTAATC 
TGCGATCAAC TTTCTTCTAA CTTCCCCCTG TGTTCTGCGT CTTGCCTTGT GTCATCCAGA 
AGCAGGCGAG CCACAGGGGC CACTGAGGAA GAGAAGATGA TAGTGAGTCT CCCGGGCCCC 
ATCCTCCTGT TGTCAGATGG CTCTTCATTC AGAGATGCTG TGGATTCTAA AGGGCATGGG 
ACTTCTGGAT ATGCTGCTTT TAAAACTATG GTTGCTGTAG TTGCCTTAGC AGGTGTTGTG 
GCAACTCTAA GCCTAATCAG CTACCTGCGC AAGAAAAGAA TCACAGTGCT AAACCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZP2 ( XM_024984639.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024840407.1 Bos taurus zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA.

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CloneID	OBa230141
Clone ID Related Accession (Same CDS sequence)	XM_024984639.1
Accession Version	XM_024984639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zona pellucida sperm-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGCAG AGAGGAGACA GCGGGAGACC CTCAAGCTGG TTCCGTGCAG ACTGGAGGTA 
TTTCTGGGGG CAGAAAGAAG GGGACAAAAT GCCTTGTCTG CTGAAGTTCA CTCCTCTTTA 
ACTTTCTCCC CCATGTCCTC CTGCACCTAC AGGTCATTTT TTCTATCTTT CACCCTTTTG 
ACTTCGGTGA ACTCAATAGA TGTTAATCAG TTGGATCCTG CCTTCCCAGG TACTGTCACT 
TGCTATGAAA ATAGAATGGT AGTGGAGTTT AAAAGAACTC TTGGCAACAA GATTCAGCAT 
GCATCTGTGG TGGATTCCCT TGGTCTTAAA ATGCTGAACT GCACTTATGT TCTGGACCCA 
GAAAAGCTCA CACTGAAGGC CCCATATGAA AGCTGTACCA AAAGAGTGCT TGGCCAGCAC 
CAGATGACCA TCACATTCAT GAATGACAAT ACTGCCCATA GACAAAAGAC TGTCTTGTAT 
CACGTCAGCT GTCCGGTTAT GCAAGCAGGA AGACATGATC AGCATTCGGG ATCCACAATC 
TGCTCGAAGG ATTTCATGTC TTTTACCTTT CACTTTTTTC CTGGCCTGGC TGACGACACT 
GCGGGTCCCA AACCTCAGAT GGGATGGACC GTTACAGTTG GTGATGGTGA AAGAGCCCAA 
AATCTGACCC TTCAAGAGGC TCTGACACAA GGATATAATC TCCTGATTGA GAACCAGAAG 
ATGAGCATCC AAGTGTTATT CCATGCCACT GGAGTGACTC ACTACTCGCA AGGTAACAGC 
CATCTCTACA TGGTGCCTCT GAAGCTTACG CATGTATCTC CTGGGCAGAC GATCATCTTA 
TCATCACGAC TCCTCTGTGC ATCAGATCCT GTGACCTGTA ATGCCACACA CATGACTCTC 
ACCATACCAG AATTTCCTGG GAAGTTAAAA TCTGTGAGCT TTGAAAATAA GAATATTGCT 
GTGAACCAGT TGCACAACAG CGGGATTGTT ATGGAAATAG CAAATGGCCT CAGGTTGCAT 
TTCAGCAAAA CTCTCCTCAA AACAAAATTC TCTGAAAAAT GCCTACCCTA TCAGTTCTAC 
TTATCTTCAC TCAAGCTGAC GTTTTATACT CAACTGGAGA CAGTATCCAT GGTGATTTAT 
CCTGAATGTG TCTGCGAGTC AACAGTTTCT ATAGTTTCAG GGGAGCTATG TACTCAAGAT 
GGGTTTATGG ACGTCGAGGT CTACCACCAT CAAACAAAAC CAGCTCTCAA CTTGGACACT 
CTTAGGGTGG GAGACTCCTC CTGCCAGCCA ACCATTAAAG CTCCATTTCA AGGACTGGTG 
AAGTTTCACA TACCCCTGAA TGGATGTGGA ACAAGACATA AGTTTGAGAA TGGCAAAGTC 
ATCTATGAAA ATGAAATACA TGCTCTCTGG GCGGATCTTC CTCCAAGCAC AATTTCCAGA 
GATAGTGAGT TCAGAATGAC GGTGAGGTGC TATTATAGCA GCAGTAACAT GCTAATAAAT 
ACCAATGTCG AAAGCCTTCC TCCTCCAGTA GCCTCAGTGA AGCCAGGTCC ACTTGCCTTG 
ACCCTGCAAA CATACCCAGA TAATTCCTAC CTGCAACCGT ACGGGGACAA GGACTATCCT 
GTGGTGAGAT ACCTCCGCCA ACCAATTTAC TTAGAAGTGA GAGTCCTAAA CAGGACTGAC 
CCCAACATCA AGTTGGTCTT AGATGATTGC TGGGCAACAT CCACCATGGA CCCAGCCTCT 
CTCCCTCAGT GGAATATTAT CGTGGATGGC TGTGAATACA ACTTGGACAA CCACAGAACC 
ACCTTCCATC CGGTTGGCTC CTCGGTGGCC TATCCTAATC ACTACCAGAG GTTTGCTGTG 
AAGACCTTTG CCTTTGTGTC AGAGGACCCG GCGTTCTCTC ACTTGGTCTA CTTCCACTGC 
AGCGCCTTAA TCTGCGATCA ACTTTCTTCT AACTTCCCCC TGTGTTCTGC GTCTTGCCTT 
GTGTCATCCA GAAGCAGGCG AGCCACAGGG GCCACTGAGG AAGAGAAGAT GATAGTGAGT 
CTCCCGGGCC CCATCCTCCT GTTGTCAGAT GGCTCTTCAT TCAGAGTGCC AGAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024840407.1
CDS	1371..3470
Translation	MQAERRQRETLKLVPCRLEVFLGAERRGQNALSAEVHSSLTFSPMSSCTYRSFFLSFTLLTSVNSIDVNQLDPAFPGTVTCYENRMVVEFKRTLGNKIQHASVVDSLGLKMLNCTYVLDPEKLTLKAPYESCTKRVLGQHQMTITFMNDNTAHRQKTVLYHVSCPVMQAGRHDQHSGSTICSKDFMSFTFHFFPGLADDTAGPKPQMGWTVTVGDGERAQNLTLQEALTQGYNLLIENQKMSIQVLFHATGVTHYSQGNSHLYMVPLKLTHVSPGQTIILSSRLLCASDPVTCNATHMTLTIPEFPGKLKSVSFENKNIAVNQLHNSGIVMEIANGLRLHFSKTLLKTKFSEKCLPYQFYLSSLKLTFYTQLETVSMVIYPECVCESTVSIVSGELCTQDGFMDVEVYHHQTKPALNLDTLRVGDSSCQPTIKAPFQGLVKFHIPLNGCGTRHKFENGKVIYENEIHALWADLPPSTISRDSEFRMTVRCYYSSSNMLINTNVESLPPPVASVKPGPLALTLQTYPDNSYLQPYGDKDYPVVRYLRQPIYLEVRVLNRTDPNIKLVLDDCWATSTMDPASLPQWNIIVDGCEYNLDNHRTTFHPVGSSVAYPNHYQRFAVKTFAFVSEDPAFSHLVYFHCSALICDQLSSNFPLCSASCLVSSRSRRATGATEEEKMIVSLPGPILLLSDGSSFRVPEC

Target ORF information:

RefSeq Version	XM_024984639.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zona pellucida glycoprotein 2 (ZP2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984639.1

ORF Insert Sequence:

ATGCAGGCAG AGAGGAGACA GCGGGAGACC CTCAAGCTGG TTCCGTGCAG ACTGGAGGTA 
TTTCTGGGGG CAGAAAGAAG GGGACAAAAT GCCTTGTCTG CTGAAGTTCA CTCCTCTTTA 
ACTTTCTCCC CCATGTCCTC CTGCACCTAC AGGTCATTTT TTCTATCTTT CACCCTTTTG 
ACTTCGGTGA ACTCAATAGA TGTTAATCAG TTGGATCCTG CCTTCCCAGG TACTGTCACT 
TGCTATGAAA ATAGAATGGT AGTGGAGTTT AAAAGAACTC TTGGCAACAA GATTCAGCAT 
GCATCTGTGG TGGATTCCCT TGGTCTTAAA ATGCTGAACT GCACTTATGT TCTGGACCCA 
GAAAAGCTCA CACTGAAGGC CCCATATGAA AGCTGTACCA AAAGAGTGCT TGGCCAGCAC 
CAGATGACCA TCACATTCAT GAATGACAAT ACTGCCCATA GACAAAAGAC TGTCTTGTAT 
CACGTCAGCT GTCCGGTTAT GCAAGCAGGA AGACATGATC AGCATTCGGG ATCCACAATC 
TGCTCGAAGG ATTTCATGTC TTTTACCTTT CACTTTTTTC CTGGCCTGGC TGACGACACT 
GCGGGTCCCA AACCTCAGAT GGGATGGACC GTTACAGTTG GTGATGGTGA AAGAGCCCAA 
AATCTGACCC TTCAAGAGGC TCTGACACAA GGATATAATC TCCTGATTGA GAACCAGAAG 
ATGAGCATCC AAGTGTTATT CCATGCCACT GGAGTGACTC ACTACTCGCA AGGTAACAGC 
CATCTCTACA TGGTGCCTCT GAAGCTTACG CATGTATCTC CTGGGCAGAC GATCATCTTA 
TCATCACGAC TCCTCTGTGC ATCAGATCCT GTGACCTGTA ATGCCACACA CATGACTCTC 
ACCATACCAG AATTTCCTGG GAAGTTAAAA TCTGTGAGCT TTGAAAATAA GAATATTGCT 
GTGAACCAGT TGCACAACAG CGGGATTGTT ATGGAAATAG CAAATGGCCT CAGGTTGCAT 
TTCAGCAAAA CTCTCCTCAA AACAAAATTC TCTGAAAAAT GCCTACCCTA TCAGTTCTAC 
TTATCTTCAC TCAAGCTGAC GTTTTATACT CAACTGGAGA CAGTATCCAT GGTGATTTAT 
CCTGAATGTG TCTGCGAGTC AACAGTTTCT ATAGTTTCAG GGGAGCTATG TACTCAAGAT 
GGGTTTATGG ACGTCGAGGT CTACCACCAT CAAACAAAAC CAGCTCTCAA CTTGGACACT 
CTTAGGGTGG GAGACTCCTC CTGCCAGCCA ACCATTAAAG CTCCATTTCA AGGACTGGTG 
AAGTTTCACA TACCCCTGAA TGGATGTGGA ACAAGACATA AGTTTGAGAA TGGCAAAGTC 
ATCTATGAAA ATGAAATACA TGCTCTCTGG GCGGATCTTC CTCCAAGCAC AATTTCCAGA 
GATAGTGAGT TCAGAATGAC GGTGAGGTGC TATTATAGCA GCAGTAACAT GCTAATAAAT 
ACCAATGTCG AAAGCCTTCC TCCTCCAGTA GCCTCAGTGA AGCCAGGTCC ACTTGCCTTG 
ACCCTGCAAA CATACCCAGA TAATTCCTAC CTGCAACCGT ACGGGGACAA GGACTATCCT 
GTGGTGAGAT ACCTCCGCCA ACCAATTTAC TTAGAAGTGA GAGTCCTAAA CAGGACTGAC 
CCCAACATCA AGTTGGTCTT AGATGATTGC TGGGCAACAT CCACCATGGA CCCAGCCTCT 
CTCCCTCAGT GGAATATTAT CGTGGATGGC TGTGAATACA ACTTGGACAA CCACAGAACC 
ACCTTCCATC CGGTTGGCTC CTCGGTGGCC TATCCTAATC ACTACCAGAG GTTTGCTGTG 
AAGACCTTTG CCTTTGTGTC AGAGGACCCG GCGTTCTCTC ACTTGGTCTA CTTCCACTGC 
AGCGCCTTAA TCTGCGATCA ACTTTCTTCT AACTTCCCCC TGTGTTCTGC GTCTTGCCTT 
GTGTCATCCA GAAGCAGGCG AGCCACAGGG GCCACTGAGG AAGAGAAGAT GATAGTGAGT 
CTCCCGGGCC CCATCCTCCT GTTGTCAGAT GGCTCTTCAT TCAGAGTGCC AGAATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
The Journal of cell biology197(1)37-44(2012 Apr)
Burkart AD,Xiong B,Baibakov B,Jim?nez-Movilla M,Dean J

Evolutionary patterns of two major reproduction candidate genes (Zp2 and Zp3) reveal no contribution to reproductive isolation between bovine species.
BMC evolutionary biology1124(2011 Jan)
Chen S,Costa V,Beja-Pereira A

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Localization of N-linked carbohydrate chains in glycoprotein ZPA of the bovine egg zona pellucida.
European journal of biochemistry269(17)4257-66(2002 Sep)
Ikeda K,Yonezawa N,Naoi K,Katsumata T,Hamano S,Nakano M

Molecular cloning of bovine zona pellucida glycoproteins ZPA and ZPB and analysis for sperm-binding component of the zona.
European journal of biochemistry268(12)3587-94(2001 Jun)
Yonezawa N,Fukui N,Kuno M,Shinoda M,Goko S,Mitsui S,Nakano M

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ZP2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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FAX

オンラインフォーム