F5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	F5
Entrez Gene ID	280687
Full Name	coagulation factor V
General protein information	Preferred Names coagulation factor V Names coagulation factor V activated protein C cofactor coagulation factor V (proaccelerin, labile factor)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173879.2	NP_776304.1	coagulation factor V precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	F5	NP_776304.1
Rattus norvegicus (Norway rat)	F5	NP_001041343.1
Gallus gallus (chicken)	F5	XP_001231901.3
Danio rerio (zebrafish)	f5	NP_001007209.2
Mus musculus (house mouse)	F5	NP_032002.1
Pan troglodytes (chimpanzee)	F5	XP_513984.4
Homo sapiens (human)	F5	NP_000121.2
	F5	XP_001093072.2
Canis lupus familiaris (dog)	F5	XP_005622605.1
Xenopus tropicalis (tropical clawed frog)	f5	XP_002935820.2

Gene Ontology
Bibliography

Related articles in PubMed

Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces.
Koklic T, Chattopadhyay R, Majumder R, Lentz BR
The Biochemical journal467(1)37-46(2015 Apr)

Identification of the MMRN1 binding region within the C2 domain of human factor V.
Jeimy SB, Woram RA, Fuller N, Quinn-Allen MA, Nicolaes GA, Dahlb?ck B, Kane WH, Hayward CP
The Journal of biological chemistry279(49)51466-71(2004 Dec)

Determination of the disulfide bridges in factor Va heavy chain.
Xue J, Kalafatis M, Silveira JR, Kung C, Mann KG
Biochemistry33(44)13109-16(1994 Nov)

The complete cDNA sequence of bovine coagulation factor V.
Guinto ER, Esmon CT, Mann KG, MacGillivray RT
The Journal of biological chemistry267(5)2971-8(1992 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest factor Xa (FXa) and factor Va (FVa) compete to bind FXa on both PS model membranes and microparticles from activated platelets; this competition between dimerization/prothrombinase complex formation appears to regulate blood coagulation.
Title: Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces.

The following F5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41458	NM_173879.2 Latest version!	Bos taurus coagulation factor V (F5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F5 ( NM_173879.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776304.1 Bos taurus coagulation factor V (F5), mRNA.

Price & Availability↑

CloneID	OBa41458
Clone ID Related Accession (Same CDS sequence)	NM_173879.2
Accession Version	NM_173879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	coagulation factor V precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M81440.1. On or before Mar 29, 2005 this sequence version replaced XM_614375.1, NM_173879.1. ##Evidence-Data-START## Transcript exon combination :: M81440.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGTTCCTCG CTTGCCCTGG CTTCTGGGTC CTCGTGGTCC TAGGCAGCAG CTGGGCAGGC 
TGGGGGAACC TAGGGGCTGA AGCAGCAAAG CTAAGGCAGT TCTACGTAGC TGCTCAGAGC 
ATCAGATGGA ACTACCGCCC CGAGTCCACA CACCTCAGTT CGAAACCTTT TGAAACCTCC 
TTTAAGAAAA TTGTCTACAG GGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACAATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTG TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAAGCACA CAAGCCCTTA AGCATCCATG CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCGTC TTACTCTGAC CACACACTCC CCATGGAGAA GATGGATGAT 
GCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGATTA TCAGTGAGCA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCTATGTAAA TCTGGTGGAG 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACCGAG 
GATGGAACTC AGAAAATGTT TGAGAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCTTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACGATGCCAG ATATAACAGT CTGTGCCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATCCAT TTCAATGGTC AGGTCCTGGA GCAGAACCAT 
CATAAGATCT CAGCCATCAC TCTCGTCAGC GCCACGTCCA CAACCGCAAA CATGACCGTG 
AGCCCCGAGG GAAGGTGGAC CATAGCTTCT CTCATCCCCA GACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GAAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AATACAGATC TCTGCATTTG 
GATAATTTCT CAAACCGAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGACTCCT TCACCAAACG CCTGGAGGAT CCCAGTAGTG AAGGAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTGAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCTCCTACA GCATTTACCC TCACGGTGTG ACATTCTCTC CTTATGACAA TGAAGTAAAC 
TCTTCCTCAA CCTCAGGCAG CAACACCATG ATCAGAGCAG TTCGACCAGG GGAAACCTAC 
ACTTATAAGT GGAACATCCT AGAATCTGAT GAACCCACAG AAAATGATGC TCAGTGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACC TTGCTTCTGG ACTGATAGGG 
CTTCTTCTAA TTTGTAAGAG CAGATCCTTG GATAGACGAG GCATACAGAG GGCAGCAGAC 
ATCGAGCAGC AGGCTGTGTT TGCCGTGTTT GACGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAAGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGTAA TTTCACTCTT CCAGCTATTA ACGGCTATGT GCCTGAGAGT 
ATACCCATAC TAGGGTTCTG CTTTGATGAC ACTGTCCAGT GGCACTTCTG CAGTGTGGGA 
ACCCAGAATG ACATTTTGAC CATTCACTTC ACTGGGCACT CATTCATCTA TGGAAAGAGG 
CACGAGGACA CCTTGACCCT TTTCCCCATG CAGGGGGAAT CCGTGACTGT CACAATGGAT 
AATGTTGGAA CTTGGATGTT AACCACCATG AATTCCAATC CAAGAAGCAA AAAACTACGG 
CTGAGGTTCA GGGATGCTAA GTGTATCCGG AATGATGATG ATGACTCCTA TGAGATTATA 
TATGAACCTT CAGGATCTAC AGCCATGACT ACAAAGAAAA TTCATGATTC TTCAGAAATC 
GAAGATGAAA ATGATGCTGA CTCTGATTAC CAGGACGAAC TGGCTTTAAT ACTAGGTCTT 
AGGTCATTCA GAAATTCATC ACTGAATCAG GAGAAAGATG AGCTCAATCT TACCGCCCTA 
GCTCTGGAGA AAGACTCTGA ATTCATTCCT CCGAGTGCCA ACAGATCTCT TGATTCAAAT 
TCTTCTTCCC GAAGTCATGT TAGCAGGCTT ATTGCCAAAA ACTTTGCAGA ATCTCTGAAA 
ACTCTTCTGC ACCTGGAAGC CCCTGCAGCT GGTTCCCCCC TGGAACACGC TGGCTTAGAT 
AAGAACTCAG CTCTCAACCC TCCCATGGCA GAGCATTCCA GCCCTTATTC TGAAGACCCT 
AGAGAAGATC ATCCACTCTC AGATGTCACA GGGGTAAGCC TACTTCCATT TGGCACAGGA 
TTCAAAAATC GAAAACCTGC CAAACATCAA AGATTCCAGG TAGGAAGAGG CCAAGCAGCA 
AAGCATAAGT TCTCCCAGAC GCGATTCCCA GCACATAAAA CCAGGACACG TTTAAGCCAA 
GACAACTCTT CTTCTTCCAG AATGGGGCCC TGGGAGGACA TTCCCAGTGA TCTGTTACTC 
TTACAACAAA AGGATCCATA TAAGATTCTG AATGGAGAAT GGCATTTGGT TTCTGAGAAA 
GGCAGTTATG AAATAATCCA AGATGCTAAT GAAAACAAGA CTGTTAATAA GTTGCCAAAC 
AGCCCCCAGA ATGACTCAAG GACTTGGGGA GAAAACATCC CTTTCAAAAA CAGTCATGGA 
AAGCAGAGTG GCCACCCAAC ATTTTTGGTA ACTAGACGTA AACCTCTACA AGACAGACAG 
GATAGAAGAA ATAGTAGATT GAAGGAAGGC CTTCCGTTAA TTAGGACACG AAGAAAGAAA 
AAGGAAGAGA AGCCTGCATA CCATGTTCCT CTATCTCCAA GGAGTTTTCA TCCTCTGAGA 
GGAGAGGTCA ATGCCTCATT TTCAGACAGA AGACATAATC ATTCATTGTT ACTCCATGCG 
TCCAATGAAA CATCTCTTTC CATAGACCTC AATCAGACAT TCCCCTCTAT GAATCTTAGC 
CTTGCAGCCT CACTTCCTGA CCATGACCAG ACCTCACCAA ATGACACCAC CAGTCAGACT 
AGCTCCCCTC CAGATCTTTA TCCGACAGTG AGCCCAGAGG AACACTATCA AATATTCCCT 
ATTCAAGACT CTGATCCAAC ACATTCTACT ACAGCCCCCA GTAACAGATC TCCTGATCCA 
ACACATTCTA CTACAGCCCC CAGTAACAGA TCTCCTCCCA CACAGCCCAG CCAGATACCC 
AACTATGACC TAAGAAACAG GGCCATCCCT ACTGATGTGA GTCAAATTTT CCCTTCCTTG 
GAACTCGAAG TCTGGCAGAC AGCTACCTCT CTAGACCTCA GTCAACCATC CATCTCCCCA 
GACCTTGGCC AGATGGCACT TTCCCCAGAC CCCGGCCAGG AGTCTCTCTC TCCAGACCTT 
GGCCAGACGT CCCTCTCTCC AGACCTCAGC CAGGAGTCTC TCTCCCCAGA CCTTGGCCAG 
ACAGCCCTTT CCCCAGACCC CAGCCAGGAG TCTCTCTCCC CAGACCTTGG CCAGACAGCC 
CTTTCCCCAG ACCCCAGCCA GGAGTCTCTC TCCCCAGACC TTGGCCAGAC AGCCCTTTCC 
CCAGACCCCG GCCAGGAGTC TCTCTCTCCA GACCTTGGCC AGACGTCCCT CTCTCCAGAC 
CTCAGCCAGG AGTCTCTCTC CCCAGACCTT GGCCAGACAG CCCTTTCCCC AGACCCCAGC 
CAGGAGTCTC TCTCCCCAGA CCTTGGCCAG ACAGCCCTTT CCCCAGACCC CAGCCAGGAG 
TCTCTCTCTC CAGACCTTGG CCAGACGTCC CTCTCTCCAG ACCTTGGCCA GGAGTCTCTC 
TCCCCAGACC TTGGCCAGAC AGCCCTTTCC CCAGACCCCA GCCAGGAGTC TCTCTCTCCA 
GACCTTGGCC AGACGTCCCT CTCTCCAGAC CTTGGCCAGG AGTCTCTCTC CCCAGACCTT 
GGCCAGACAG CCCTTTCCCC AGACCTCAGC CAGGAGTCTC TCTCTCCAGA TCTTGGCCAG 
ACACCCCTCT CTCCAGACCT CAGCCTGGAG TCTCTTTCTC CAGACCTCAG CCAGCTTGAT 
CTCAAGCAGA CATCACCTCC TCTAGATCTT AATCAGACAT CCCACACTTC TGAATCAAGT 
CAGTCATTGC CTCTTCCAGA ATTTGGTCAG ACTTTCCCTA ATGCAGATAT TGGTCAGATG 
CCATCTCCTC CACCAGACTC TACACTAAAT AACACTTTTA TACCAGAAGA ATTTAATCCG 
CTGGTTGTAG TAGGCCTCAG TAGAGATGAT GGAGATTATA TTGAAATTAT TCCAAGGCAG 
AAGGAAGAGA GCAGTGAAGA AGACTATGGT GAATTTGAGT TTGTAGCCTA TAATGACCCT 
TACCAAACTG ATCTTAGGAC AGATATCAAC TCCTCCAGAA ATCCTGACAA CATTGCAGCA 
TGGTACCTCC GCAGCAACAC TGGAAACAGA AAATATTATT ACATTGCAGC TGAAGAAATA 
TCCTGGGATT ATTCAAAATT TGTGCAAAGT GATGACGTTG ACTATGTTCC AGAGGACACC 
GTATACAAGA AAGTAGTTTT CCGAAAGTAC CTTGATAGCA CTTTTACCAA ACTTGATCCT 
CAGGGGGAGT ATGAAGAGCA TCTTGGCATA CTTGGTCCAG TCATTAGAGC TGAAGTGGAT 
GATGTTATCC AAGTTCGTTT TAAAAATTTA GCATCCAGAC CATATTCTCT TCATGCCCAT 
GGGCTTTCCT ATGAAAAATC ATCAGAAGGA AAGACTTATG AAGATGACTC TCCTGAATGG 
TTTAAGGAGG ACAATGCTAT TCAGCCCAAT AAAACTTACA CCTATGTATG GCACGCCACT 
ACGCGATCCG GGCCAGAAAA CCCTGGATCT GCCTGTCGGG CTTGGGCCTA CTACTCAGCA 
GTGAACCCAG AAAAAGACAT CCATTCAGGC TTGATAGGGC CTCTTCTGAT CTGCCGAAAA 
GGGACACTTG ATAAGGAGAC CAACATGCCT GTGGACATGA GAGAATTTGT CCTGCTTTTT 
ATGGTCTTTG ATGAAAAGAA GAGCTGGTAT TATGACAAGA AGCCCACAAG GTCTTGGAGA 
CGTGCATCCT CAGAAGTAAA AAACTCCCAT GAGTTTCATG CCATCAATGG GATGATCTAC 
AACTTGCCTG GCTTGAGAAT GTACGAGCAA GAGTGGGTGA GGTTGCACCT GCTGAACTTA 
GGCGGCTCCC GAGACATTCA CGTGGTTCAC TTTCATGGCC AGACCTTGCT AGAAAACGGC 
ACTCAACAGC ACCAGTTAGG GGTCTGGCCC CTTCTGCCTG GTTCATTTAA AACTCTTGAA 
ATGAAGGCAT CAAAACCTGG CTGGTGGCTC CTAGACACGG AAGTTGGAGA AATTCAGAGA 
GCAGGGATGC AGACACCATT TCTCATTGTA GACAGAGAAT GTAAGATGCC AATGGGACTA 
AGCACTGGCC TGATAGCTGA CTCACAGATC CAGGCTTCTG AGTTTTGGGG TTATTGGGAA 
CCCAAATTAG CAAGGTTAAA CAATGGTGGA TCATACAATG CTTGGATTGC AGAAAAACTT 
TCAACGGAAT TTAACCCTGA ACCTTGGATC CAGGTAGACA TGCAAAAGGA AGTCCTGCTC 
ACGGGGATCC AGACCCAGGG CGCCAAACAC TACCTGAAGC CCTACTACAC CACCGAGTTC 
TGTGTGGCTT ACAGCTTGGA TCGGAAAAAC TGGCGTATCT TCAAAGGGAA CAGCACAAGG 
AATGTGATGT ATTTTGGTGG CAATTCAGAT GCTTCTACAA TAAAAGAGAA TCAGATTGAC 
CCACCTGTTG TGGCTAGATA CATTAGGATC TCTCCAACTG GATCCTATAA CAAACCTGCC 
CTTCGATTGG AGCTGCAAGG TTGTGAGGTT AATGGATGCT CCACACCGCT GGGTATGGAA 
AGTGGAAAGA TAGAAAACAA GCAAATCACC GCTTCCTCGT TTAAAAAGTC TTGGTGGGGA 
AATTACTGGG AACCCTTCCT TGCACGTCTT AATGCCCAGG GCCGTGTAAA TGCCTGGCAA 
GCTAAGGCAA ACAACAACAA TCAGTGGTTA CAAATTGATC TGCTCAAAAT CAAGAAGATA 
ACTGCGATTG TAACACAAGG ATGCAAGTCT CTGTCCTCTG AAATGTATGT GAAGAGCTAC 
ACCATCCACT ACAGTGACCA GGGAACGGAC TGGAAACCTT ACAGGGAGAA ATCCTCAATG 
GTGGACAAGA TTTTCGAAGG AAATAATAAT GTCAGAGGAC ATGTGAAGAA CTTTTTCAAC 
CCACCAATCA TCTCCAGGTT TATACGCATC ATTCCTAAAA CATGGAATCA GAGTATTGCA 
CTTCGCTTGG AACTCTTTGG CTGTGATATG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776304.1
CDS	104..6739
Translation	MFLACPGFWVLVVLGSSWAGWGNLGAEAAKLRQFYVAAQSIRWNYRPESTHLSSKPFETSFKKIVYREYEAYFQKEKPQSRTSGLLGPTLYAEVGDIMKVHFKNKAHKPLSIHAQGIKYSKFSEGASYSDHTLPMEKMDDAVAPGQEYTYEWIISEHSGPTHDDPPCLTHIYYSYVNLVEDFNSGLIGPLLICKKGTLTEDGTQKMFEKQHVLMFAVFDESKSWNQTSSLMYTVNGYVNGTMPDITVCAHDHISWHLIGMSSGPELFSIHFNGQVLEQNHHKISAITLVSATSTTANMTVSPEGRWTIASLIPRHFQAGMQAYIDIKNCAKKTRNPKKLTRDQRRHIKRWEYFIAAEEVIWDYAPIIPANMDKKYRSLHLDNFSNRIGKHYKKVVYKQYQDDSFTKRLEDPSSEGDGILGPIIRAQVRDTLKIVFKNMASRSYSIYPHGVTFSPYDNEVNSSSTSGSNTMIRAVRPGETYTYKWNILESDEPTENDAQCLTRPYYSNVDITRDLASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYIEDNIYKFCENPEKVKRDDPKFYESNIMSNFTLPAINGYVPESIPILGFCFDDTVQWHFCSVGTQNDILTIHFTGHSFIYGKRHEDTLTLFPMQGESVTVTMDNVGTWMLTTMNSNPRSKKLRLRFRDAKCIRNDDDDSYEIIYEPSGSTAMTTKKIHDSSEIEDENDADSDYQDELALILGLRSFRNSSLNQEKDELNLTALALEKDSEFIPPSANRSLDSNSSSRSHVSRLIAKNFAESLKTLLHLEAPAAGSPLEHAGLDKNSALNPPMAEHSSPYSEDPREDHPLSDVTGVSLLPFGTGFKNRKPAKHQRFQVGRGQAAKHKFSQTRFPAHKTRTRLSQDNSSSSRMGPWEDIPSDLLLLQQKDPYKILNGEWHLVSEKGSYEIIQDANENKTVNKLPNSPQNDSRTWGENIPFKNSHGKQSGHPTFLVTRRKPLQDRQDRRNSRLKEGLPLIRTRRKKKEEKPAYHVPLSPRSFHPLRGEVNASFSDRRHNHSLLLHASNETSLSIDLNQTFPSMNLSLAASLPDHDQTSPNDTTSQTSSPPDLYPTVSPEEHYQIFPIQDSDPTHSTTAPSNRSPDPTHSTTAPSNRSPPTQPSQIPNYDLRNRAIPTDVSQIFPSLELEVWQTATSLDLSQPSISPDLGQMALSPDPGQESLSPDLGQTSLSPDLSQESLSPDLGQTALSPDPSQESLSPDLGQTALSPDPSQESLSPDLGQTALSPDPGQESLSPDLGQTSLSPDLSQESLSPDLGQTALSPDPSQESLSPDLGQTALSPDPSQESLSPDLGQTSLSPDLGQESLSPDLGQTALSPDPSQESLSPDLGQTSLSPDLGQESLSPDLGQTALSPDLSQESLSPDLGQTPLSPDLSLESLSPDLSQLDLKQTSPPLDLNQTSHTSESSQSLPLPEFGQTFPNADIGQMPSPPPDSTLNNTFIPEEFNPLVVVGLSRDDGDYIEIIPRQKEESSEEDYGEFEFVAYNDPYQTDLRTDINSSRNPDNIAAWYLRSNTGNRKYYYIAAEEISWDYSKFVQSDDVDYVPEDTVYKKVVFRKYLDSTFTKLDPQGEYEEHLGILGPVIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKSSEGKTYEDDSPEWFKEDNAIQPNKTYTYVWHATTRSGPENPGSACRAWAYYSAVNPEKDIHSGLIGPLLICRKGTLDKETNMPVDMREFVLLFMVFDEKKSWYYDKKPTRSWRRASSEVKNSHEFHAINGMIYNLPGLRMYEQEWVRLHLLNLGGSRDIHVVHFHGQTLLENGTQQHQLGVWPLLPGSFKTLEMKASKPGWWLLDTEVGEIQRAGMQTPFLIVDRECKMPMGLSTGLIADSQIQASEFWGYWEPKLARLNNGGSYNAWIAEKLSTEFNPEPWIQVDMQKEVLLTGIQTQGAKHYLKPYYTTEFCVAYSLDRKNWRIFKGNSTRNVMYFGGNSDASTIKENQIDPPVVARYIRISPTGSYNKPALRLELQGCEVNGCSTPLGMESGKIENKQITASSFKKSWWGNYWEPFLARLNAQGRVNAWQAKANNNNQWLQIDLLKIKKITAIVTQGCKSLSSEMYVKSYTIHYSDQGTDWKPYREKSSMVDKIFEGNNNVRGHVKNFFNPPIISRFIRIIPKTWNQSIALRLELFGCDMY

Target ORF information:

RefSeq Version	NM_173879.2
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor V (F5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173879.2

ORF Insert Sequence:

ATGTTCCTCG CTTGCCCTGG CTTCTGGGTC CTCGTGGTCC TAGGCAGCAG CTGGGCAGGC 
TGGGGGAACC TAGGGGCTGA AGCAGCAAAG CTAAGGCAGT TCTACGTAGC TGCTCAGAGC 
ATCAGATGGA ACTACCGCCC CGAGTCCACA CACCTCAGTT CGAAACCTTT TGAAACCTCC 
TTTAAGAAAA TTGTCTACAG GGAGTATGAA GCATATTTTC AGAAAGAAAA ACCACAATCC 
AGAACTTCAG GACTTCTTGG GCCTACTTTG TATGCTGAAG TTGGAGACAT CATGAAAGTT 
CACTTTAAGA ATAAAGCACA CAAGCCCTTA AGCATCCATG CTCAAGGAAT TAAGTACAGT 
AAATTCTCAG AAGGTGCGTC TTACTCTGAC CACACACTCC CCATGGAGAA GATGGATGAT 
GCTGTAGCTC CGGGCCAAGA ATATACCTAT GAGTGGATTA TCAGTGAGCA CAGTGGGCCC 
ACCCACGATG ACCCTCCATG CCTCACACAC ATCTATTACT CCTATGTAAA TCTGGTGGAG 
GACTTCAACT CTGGACTGAT TGGACCTCTG CTTATTTGTA AGAAAGGCAC CCTAACCGAG 
GATGGAACTC AGAAAATGTT TGAGAAGCAA CATGTACTGA TGTTTGCTGT GTTTGATGAA 
AGTAAAAGCT GGAACCAGAC ATCATCCTTA ATGTACACAG TCAATGGCTA TGTGAATGGG 
ACGATGCCAG ATATAACAGT CTGTGCCCAT GACCACATCA GTTGGCATCT GATTGGAATG 
AGCTCTGGGC CAGAACTGTT CTCCATCCAT TTCAATGGTC AGGTCCTGGA GCAGAACCAT 
CATAAGATCT CAGCCATCAC TCTCGTCAGC GCCACGTCCA CAACCGCAAA CATGACCGTG 
AGCCCCGAGG GAAGGTGGAC CATAGCTTCT CTCATCCCCA GACATTTTCA AGCTGGGATG 
CAGGCTTACA TAGACATTAA AAACTGTGCA AAGAAAACCA GAAATCCTAA GAAACTAACT 
CGAGACCAGA GGCGGCACAT TAAGAGATGG GAATACTTCA TTGCTGCAGA GGAAGTCATT 
TGGGACTATG CACCTATAAT ACCAGCAAAC ATGGACAAAA AATACAGATC TCTGCATTTG 
GATAATTTCT CAAACCGAAT TGGAAAACAT TATAAGAAGG TTGTCTACAA ACAGTACCAA 
GATGACTCCT TCACCAAACG CCTGGAGGAT CCCAGTAGTG AAGGAGATGG GATCTTGGGC 
CCTATTATCA GAGCCCAGGT CAGAGACACA CTGAAAATCG TGTTCAAAAA TATGGCCAGC 
CGCTCCTACA GCATTTACCC TCACGGTGTG ACATTCTCTC CTTATGACAA TGAAGTAAAC 
TCTTCCTCAA CCTCAGGCAG CAACACCATG ATCAGAGCAG TTCGACCAGG GGAAACCTAC 
ACTTATAAGT GGAACATCCT AGAATCTGAT GAACCCACAG AAAATGATGC TCAGTGCTTA 
ACAAGACCAT ACTACAGTAA TGTGGACATC ACAAGGGACC TTGCTTCTGG ACTGATAGGG 
CTTCTTCTAA TTTGTAAGAG CAGATCCTTG GATAGACGAG GCATACAGAG GGCAGCAGAC 
ATCGAGCAGC AGGCTGTGTT TGCCGTGTTT GACGAGAACA AGAGCTGGTA CATTGAGGAC 
AACATCTACA AGTTTTGTGA AAATCCTGAG AAAGTGAAAC GTGATGACCC CAAGTTTTAT 
GAGTCAAACA TCATGAGTAA TTTCACTCTT CCAGCTATTA ACGGCTATGT GCCTGAGAGT 
ATACCCATAC TAGGGTTCTG CTTTGATGAC ACTGTCCAGT GGCACTTCTG CAGTGTGGGA 
ACCCAGAATG ACATTTTGAC CATTCACTTC ACTGGGCACT CATTCATCTA TGGAAAGAGG 
CACGAGGACA CCTTGACCCT TTTCCCCATG CAGGGGGAAT CCGTGACTGT CACAATGGAT 
AATGTTGGAA CTTGGATGTT AACCACCATG AATTCCAATC CAAGAAGCAA AAAACTACGG 
CTGAGGTTCA GGGATGCTAA GTGTATCCGG AATGATGATG ATGACTCCTA TGAGATTATA 
TATGAACCTT CAGGATCTAC AGCCATGACT ACAAAGAAAA TTCATGATTC TTCAGAAATC 
GAAGATGAAA ATGATGCTGA CTCTGATTAC CAGGACGAAC TGGCTTTAAT ACTAGGTCTT 
AGGTCATTCA GAAATTCATC ACTGAATCAG GAGAAAGATG AGCTCAATCT TACCGCCCTA 
GCTCTGGAGA AAGACTCTGA ATTCATTCCT CCGAGTGCCA ACAGATCTCT TGATTCAAAT 
TCTTCTTCCC GAAGTCATGT TAGCAGGCTT ATTGCCAAAA ACTTTGCAGA ATCTCTGAAA 
ACTCTTCTGC ACCTGGAAGC CCCTGCAGCT GGTTCCCCCC TGGAACACGC TGGCTTAGAT 
AAGAACTCAG CTCTCAACCC TCCCATGGCA GAGCATTCCA GCCCTTATTC TGAAGACCCT 
AGAGAAGATC ATCCACTCTC AGATGTCACA GGGGTAAGCC TACTTCCATT TGGCACAGGA 
TTCAAAAATC GAAAACCTGC CAAACATCAA AGATTCCAGG TAGGAAGAGG CCAAGCAGCA 
AAGCATAAGT TCTCCCAGAC GCGATTCCCA GCACATAAAA CCAGGACACG TTTAAGCCAA 
GACAACTCTT CTTCTTCCAG AATGGGGCCC TGGGAGGACA TTCCCAGTGA TCTGTTACTC 
TTACAACAAA AGGATCCATA TAAGATTCTG AATGGAGAAT GGCATTTGGT TTCTGAGAAA 
GGCAGTTATG AAATAATCCA AGATGCTAAT GAAAACAAGA CTGTTAATAA GTTGCCAAAC 
AGCCCCCAGA ATGACTCAAG GACTTGGGGA GAAAACATCC CTTTCAAAAA CAGTCATGGA 
AAGCAGAGTG GCCACCCAAC ATTTTTGGTA ACTAGACGTA AACCTCTACA AGACAGACAG 
GATAGAAGAA ATAGTAGATT GAAGGAAGGC CTTCCGTTAA TTAGGACACG AAGAAAGAAA 
AAGGAAGAGA AGCCTGCATA CCATGTTCCT CTATCTCCAA GGAGTTTTCA TCCTCTGAGA 
GGAGAGGTCA ATGCCTCATT TTCAGACAGA AGACATAATC ATTCATTGTT ACTCCATGCG 
TCCAATGAAA CATCTCTTTC CATAGACCTC AATCAGACAT TCCCCTCTAT GAATCTTAGC 
CTTGCAGCCT CACTTCCTGA CCATGACCAG ACCTCACCAA ATGACACCAC CAGTCAGACT 
AGCTCCCCTC CAGATCTTTA TCCGACAGTG AGCCCAGAGG AACACTATCA AATATTCCCT 
ATTCAAGACT CTGATCCAAC ACATTCTACT ACAGCCCCCA GTAACAGATC TCCTGATCCA 
ACACATTCTA CTACAGCCCC CAGTAACAGA TCTCCTCCCA CACAGCCCAG CCAGATACCC 
AACTATGACC TAAGAAACAG GGCCATCCCT ACTGATGTGA GTCAAATTTT CCCTTCCTTG 
GAACTCGAAG TCTGGCAGAC AGCTACCTCT CTAGACCTCA GTCAACCATC CATCTCCCCA 
GACCTTGGCC AGATGGCACT TTCCCCAGAC CCCGGCCAGG AGTCTCTCTC TCCAGACCTT 
GGCCAGACGT CCCTCTCTCC AGACCTCAGC CAGGAGTCTC TCTCCCCAGA CCTTGGCCAG 
ACAGCCCTTT CCCCAGACCC CAGCCAGGAG TCTCTCTCCC CAGACCTTGG CCAGACAGCC 
CTTTCCCCAG ACCCCAGCCA GGAGTCTCTC TCCCCAGACC TTGGCCAGAC AGCCCTTTCC 
CCAGACCCCG GCCAGGAGTC TCTCTCTCCA GACCTTGGCC AGACGTCCCT CTCTCCAGAC 
CTCAGCCAGG AGTCTCTCTC CCCAGACCTT GGCCAGACAG CCCTTTCCCC AGACCCCAGC 
CAGGAGTCTC TCTCCCCAGA CCTTGGCCAG ACAGCCCTTT CCCCAGACCC CAGCCAGGAG 
TCTCTCTCTC CAGACCTTGG CCAGACGTCC CTCTCTCCAG ACCTTGGCCA GGAGTCTCTC 
TCCCCAGACC TTGGCCAGAC AGCCCTTTCC CCAGACCCCA GCCAGGAGTC TCTCTCTCCA 
GACCTTGGCC AGACGTCCCT CTCTCCAGAC CTTGGCCAGG AGTCTCTCTC CCCAGACCTT 
GGCCAGACAG CCCTTTCCCC AGACCTCAGC CAGGAGTCTC TCTCTCCAGA TCTTGGCCAG 
ACACCCCTCT CTCCAGACCT CAGCCTGGAG TCTCTTTCTC CAGACCTCAG CCAGCTTGAT 
CTCAAGCAGA CATCACCTCC TCTAGATCTT AATCAGACAT CCCACACTTC TGAATCAAGT 
CAGTCATTGC CTCTTCCAGA ATTTGGTCAG ACTTTCCCTA ATGCAGATAT TGGTCAGATG 
CCATCTCCTC CACCAGACTC TACACTAAAT AACACTTTTA TACCAGAAGA ATTTAATCCG 
CTGGTTGTAG TAGGCCTCAG TAGAGATGAT GGAGATTATA TTGAAATTAT TCCAAGGCAG 
AAGGAAGAGA GCAGTGAAGA AGACTATGGT GAATTTGAGT TTGTAGCCTA TAATGACCCT 
TACCAAACTG ATCTTAGGAC AGATATCAAC TCCTCCAGAA ATCCTGACAA CATTGCAGCA 
TGGTACCTCC GCAGCAACAC TGGAAACAGA AAATATTATT ACATTGCAGC TGAAGAAATA 
TCCTGGGATT ATTCAAAATT TGTGCAAAGT GATGACGTTG ACTATGTTCC AGAGGACACC 
GTATACAAGA AAGTAGTTTT CCGAAAGTAC CTTGATAGCA CTTTTACCAA ACTTGATCCT 
CAGGGGGAGT ATGAAGAGCA TCTTGGCATA CTTGGTCCAG TCATTAGAGC TGAAGTGGAT 
GATGTTATCC AAGTTCGTTT TAAAAATTTA GCATCCAGAC CATATTCTCT TCATGCCCAT 
GGGCTTTCCT ATGAAAAATC ATCAGAAGGA AAGACTTATG AAGATGACTC TCCTGAATGG 
TTTAAGGAGG ACAATGCTAT TCAGCCCAAT AAAACTTACA CCTATGTATG GCACGCCACT 
ACGCGATCCG GGCCAGAAAA CCCTGGATCT GCCTGTCGGG CTTGGGCCTA CTACTCAGCA 
GTGAACCCAG AAAAAGACAT CCATTCAGGC TTGATAGGGC CTCTTCTGAT CTGCCGAAAA 
GGGACACTTG ATAAGGAGAC CAACATGCCT GTGGACATGA GAGAATTTGT CCTGCTTTTT 
ATGGTCTTTG ATGAAAAGAA GAGCTGGTAT TATGACAAGA AGCCCACAAG GTCTTGGAGA 
CGTGCATCCT CAGAAGTAAA AAACTCCCAT GAGTTTCATG CCATCAATGG GATGATCTAC 
AACTTGCCTG GCTTGAGAAT GTACGAGCAA GAGTGGGTGA GGTTGCACCT GCTGAACTTA 
GGCGGCTCCC GAGACATTCA CGTGGTTCAC TTTCATGGCC AGACCTTGCT AGAAAACGGC 
ACTCAACAGC ACCAGTTAGG GGTCTGGCCC CTTCTGCCTG GTTCATTTAA AACTCTTGAA 
ATGAAGGCAT CAAAACCTGG CTGGTGGCTC CTAGACACGG AAGTTGGAGA AATTCAGAGA 
GCAGGGATGC AGACACCATT TCTCATTGTA GACAGAGAAT GTAAGATGCC AATGGGACTA 
AGCACTGGCC TGATAGCTGA CTCACAGATC CAGGCTTCTG AGTTTTGGGG TTATTGGGAA 
CCCAAATTAG CAAGGTTAAA CAATGGTGGA TCATACAATG CTTGGATTGC AGAAAAACTT 
TCAACGGAAT TTAACCCTGA ACCTTGGATC CAGGTAGACA TGCAAAAGGA AGTCCTGCTC 
ACGGGGATCC AGACCCAGGG CGCCAAACAC TACCTGAAGC CCTACTACAC CACCGAGTTC 
TGTGTGGCTT ACAGCTTGGA TCGGAAAAAC TGGCGTATCT TCAAAGGGAA CAGCACAAGG 
AATGTGATGT ATTTTGGTGG CAATTCAGAT GCTTCTACAA TAAAAGAGAA TCAGATTGAC 
CCACCTGTTG TGGCTAGATA CATTAGGATC TCTCCAACTG GATCCTATAA CAAACCTGCC 
CTTCGATTGG AGCTGCAAGG TTGTGAGGTT AATGGATGCT CCACACCGCT GGGTATGGAA 
AGTGGAAAGA TAGAAAACAA GCAAATCACC GCTTCCTCGT TTAAAAAGTC TTGGTGGGGA 
AATTACTGGG AACCCTTCCT TGCACGTCTT AATGCCCAGG GCCGTGTAAA TGCCTGGCAA 
GCTAAGGCAA ACAACAACAA TCAGTGGTTA CAAATTGATC TGCTCAAAAT CAAGAAGATA 
ACTGCGATTG TAACACAAGG ATGCAAGTCT CTGTCCTCTG AAATGTATGT GAAGAGCTAC 
ACCATCCACT ACAGTGACCA GGGAACGGAC TGGAAACCTT ACAGGGAGAA ATCCTCAATG 
GTGGACAAGA TTTTCGAAGG AAATAATAAT GTCAGAGGAC ATGTGAAGAA CTTTTTCAAC 
CCACCAATCA TCTCCAGGTT TATACGCATC ATTCCTAAAA CATGGAATCA GAGTATTGCA 
CTTCGCTTGG AACTCTTTGG CTGTGATATG TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Factor Xa dimerization competes with prothrombinase complex formation on platelet-like membrane surfaces.
The Biochemical journal467(1)37-46(2015 Apr)
Koklic T,Chattopadhyay R,Majumder R,Lentz BR

Identification of the MMRN1 binding region within the C2 domain of human factor V.
The Journal of biological chemistry279(49)51466-71(2004 Dec)
Jeimy SB,Woram RA,Fuller N,Quinn-Allen MA,Nicolaes GA,Dahlb?ck B,Kane WH,Hayward CP

Determination of the disulfide bridges in factor Va heavy chain.
Biochemistry33(44)13109-16(1994 Nov)
Xue J,Kalafatis M,Silveira JR,Kung C,Mann KG

The complete cDNA sequence of bovine coagulation factor V.
The Journal of biological chemistry267(5)2971-8(1992 Feb)
Guinto ER,Esmon CT,Mann KG,MacGillivray RT

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F5 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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