ME2 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	ME2
Entrez Gene ID	109577832
Full Name	malic enzyme 2
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019986706.1	XP_019842265.1	NAD-dependent malic enzyme, mitochondrial

Pathways from BioSystems
Gene Ontology

The following ME2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ME2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo42593	XM_019986706.1 Latest version!	Bos indicus malic enzyme 2 (ME2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ME2 ( XM_019986706.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019842265.1 Bos indicus malic enzyme 2 (ME2), mRNA.

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CloneID	OBo42593
Clone ID Related Accession (Same CDS sequence)	XM_019986706.1
Accession Version	XM_019986706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	NAD-dependent malic enzyme, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032673.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC 
ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA 
CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA 
AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG 
CAAGATGATA TTGAAACYCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC 
TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG 
ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA 
GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT 
GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT 
TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG 
GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT 
CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA 
CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA 
AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA 
ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT 
CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA 
GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT 
CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT 
AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG 
TGTTTGTTTG CCAGTGGCAG TCCGTTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT 
ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT 
CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG 
GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT 
TTCCGATACC CAGAACCTGA GGASAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC 
GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCRGC GCACCCTCCC 
CCCATAACAG AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019842265.1
CDS	43..1797
Translation	MLSRFKIVATPCTLACRHLHTKEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKLNGPLEKYIYIMGIQERNEKLFYRILQDDIEnullLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNPALLKDPFYMGLYQKRDRSQRYDDLIDEFMKAITDRYGQNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGLLAAQKVIGKPISDHRILFLGAGEAALGIANLIVMAMVENGLSEEEARKKIWMVDKFGLLFKGRKAKIESHQEAFAHSAPESLPDTFEEAVNILKPSAIIGVAGAGRLFTPGVIKAMAEINERPIIFALSNPTAQAECTAEEAYTLTEGRCLFASGSPFEPVKLSDGQVFTPGQGNNVYIFPGVALAVILCNTRHISDHVFLEAAKALTSQLTDKELAQGRLYPPLSNIQEVSINIAIKVTEYLYANKMAFRYPEPEnullKAKYIRERIWRSEYDSLLPDVYEWPEAnullAHPPPITE

Target ORF information:

RefSeq Version	XM_019986706.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus malic enzyme 2 (ME2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019986706.1

ORF Insert Sequence:

ATGCTGTCCA GGTTTAAAAT AGTCGCCACT CCTTGTACTC TGGCATGCCG CCATTTGCAC 
ACAAAAGAGA AAGGCAAGCC ACTTATGCTG AACCCAAGAA CAAACAAGGG GATGGCGTTC 
ACATTACAGG AAAGACAAAT GCTTGGTCTT CAAGGACTTC TACCTCCCAA AATAGAGACA 
CAAGATATTC AAGCCTTACG GTTCCATAGA AACTTGAAAA AGTTGAATGG CCCTTTGGAA 
AAATACATCT ATATAATGGG AATACAAGAA AGAAATGAGA AATTGTTTTA CAGAATACTG 
CAAGATGATA TTGAAACYCT AATGCCAATT GTGTATACAC CAACAGTTGG CCTTGCCTGC 
TCCCAGTATG GACACATCTT TAGAAGACCT AAGGGATTAT TTATTTCAAT CTCAGACAGA 
GGTCATGTTA GATCAATTGT GGATAACTGG CCAGAAAATC ATGTTAAGGC TGTCGTGGTG 
ACTGATGGAG AGAGAATTCT AGGTCTTGGA GATCTGGGTG TCTATGGAAT GGGAATTCCA 
GTAGGAAAAC TTTGTTTGTA TACAGCTTGT GCGGGAATAC GGCCTGATAG ATGTCTGCCT 
GTGTGTATTG ACGTAGGAAC TGATAATCCG GCCCTCTTAA AGGATCCATT TTATATGGGT 
TTATATCAGA AAAGAGATCG ATCACAGCGT TACGATGACC TAATTGATGA GTTTATGAAG 
GCCATTACTG ACAGATATGG ACAGAACACA CTTATTCAGT TTGAAGACTT TGGAAATCAT 
AATGCATTCA GGTTCTTGAG AAAATACCGA GAAAAATACT GTACCTTCAA CGATGATATT 
CAAGGGACGG CTGCGGTGGC TCTGGCCGGT CTGCTTGCCG CGCAAAAAGT CATTGGGAAA 
CCGATCTCAG ATCATAGAAT CTTATTTCTT GGAGCAGGAG AGGCTGCTCT TGGAATTGCA 
AATCTGATAG TTATGGCTAT GGTTGAAAAT GGCCTCTCAG AAGAAGAGGC ACGAAAGAAA 
ATTTGGATGG TTGACAAGTT TGGTTTATTA TTTAAGGGGC GGAAAGCTAA AATAGAAAGT 
CATCAGGAAG CATTTGCTCA CTCAGCTCCA GAGAGCCTAC CTGATACTTT TGAAGAGGCA 
GTAAATATAC TTAAGCCTTC AGCTATAATT GGAGTTGCAG GAGCTGGCCG ACTTTTCACT 
CCTGGTGTAA TCAAAGCAAT GGCTGAAATC AATGAAAGGC CTATAATATT TGCATTAAGT 
AATCCTACGG CACAGGCTGA GTGCACAGCT GAAGAAGCAT ACACGCTTAC AGAGGGAAGG 
TGTTTGTTTG CCAGTGGCAG TCCGTTTGAG CCAGTGAAAC TCAGTGATGG GCAAGTCTTT 
ACACCAGGCC AAGGAAACAA TGTATATATT TTTCCAGGTG TGGCTTTAGC TGTTATTCTC 
TGTAACACCC GGCATATTAG TGACCATGTG TTCCTGGAAG CTGCAAAGGC TCTGACAAGT 
CAGTTGACAG ATAAAGAGCT GGCTCAGGGG AGACTTTACC CACCACTTTC TAATATTCAG 
GAAGTTTCTA TTAACATTGC TATTAAAGTT ACAGAATACC TGTATGCTAA TAAAATGGCT 
TTCCGATACC CAGAACCTGA GGASAAGGCC AAATACATTA GAGAAAGAAT ATGGCGAAGC 
GAGTACGATT CCCTGCTGCC CGATGTGTAT GAGTGGCCAG AGGCTGCRGC GCACCCTCCC 
CCCATAACAG AATAG