AARS2 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	AARS2
Entrez Gene ID	109577289
Full Name	alanyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019986177.1	XP_019841736.1	alanine--tRNA ligase, mitochondrial isoform X1
XM_019986178.1	XP_019841737.1	alanine--tRNA ligase, mitochondrial isoform X2

Pathways from BioSystems
Gene Ontology

The following AARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo42193	XM_019986177.1 Latest version!	Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo42194	XM_019986178.1 Latest version!	Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AARS2 ( XM_019986177.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019841736.1 Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo42193
Clone ID Related Accession (Same CDS sequence)	XM_019986177.1
Accession Version	XM_019986177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	alanine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032672.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGT TCAAGCCAAT CTTCCTGGGC ACTGTGGATC CACGAAGCGA GATGGCAGGC 
TTCCGGCGGG TAGCCAACAG CCAGAAATGT GTGAGGGCTG GAGGACGCCA CAGCGACCTG 
GAAGATGTGG GCCGAGACCT TTCCCATCAT ACCTTCTTCG AGATGCTGGG CAATTGGGCC 
TTTGGGGGTG AATATTTTAA GGAGGAGGCT TGCAGCATGG CCTGGGAGCT GCTGACTCAG 
GTCTATGGGA TCCCTGAGGA CAGGCTCTGG GTCTCCTACT TTGGTGGGGA CCCCAAGGCT 
GGGCTGGGCC CAGACTTGGA GAGCCGGGAC ATCTGGCTCA GCTTGGGGGT GCCTGCCAGC 
CGTGTGCTCT CCTTTGGGCC CGAGGAGAAC TTCTGGGAGA TGGGGGATAC TGGGCCTTGT 
GGGCCCTGCA CTGAGATACA CTATGACCTG GCCGGTGGGG CAGGGGCCCC CCAGCTGGTA 
GAGCTTTGGA ATCTGGTCTT CATGCAACAC AACAGAGAGG CAGACGGAAG CCTGCAGCCT 
CTGCCCCAGC GGCACGTGGA CACAGGAATG GGCCTAGAAA GGCTGGTGGC YGTGCTGCAA 
GGCAGGCTCT CCACCTATGA CACCGACCTG TTTTCCCCGC TGCTCGACGC CATACACCAG 
GGTTGCGGGG CACCCCCTTA CTCAGGCCGC ATTGGGGCAG CAGATGAGGG GCGCACAGAC 
ACAGCATACC GCGTGGTGGC TGATCACATC CGCACACTCA GTGTCTGCAT CGCTGACGGC 
GTCTCCCCTG GGATGTCAGG CGCYCCGCTG GTACTTCGTC GGATCCTCCG TCGAGCTGTG 
CGGTTCTCTA CTGAGGTGTT GCGGGCACCA CCTGGCTTCC TGGGCAGCCT GGTGCCTGTA 
GTGGTGGAGA CACTGGGAGA TGCTTACCCA GAATTGCGGA GGAGCTCAGC CCAGATAGCC 
AGCCTGGTGT CGGAGGATGA GGCAGCCTTC CTGGCCTCAC TGCAGCGTGG TCGGCGGGTC 
ATCGAGCGGA CCCTGAAGCG CCTGGGGCCT TCTGATGTGT TCCCAGCCGA AGTGGCCTGG 
TCCTTGTCAC TGTCTGGGAA CCTGGGGCTC CCCCTGGACC TGGTGGAGCT GATGCTGGAG 
GAGAAGGGGG TGCCGCTGGA CACTGCTGGA CTGGAGCGGC TGGCCCAGGA GGAGGCCCAG 
CACCGGGCAC TGCAGGCCGA GCCGGTTCAG GAGCAGGGAC TGCGACTCAA CGTCCACGCC 
CTGGGGGAGC TGCAGCGCCG AGGGGTGCCC CCAACCGATG ATAGCCCCAA GTACAGCTAC 
TCCCTGCAAC CAGGCGGCAG TTACATCTCG GCCCTGGCAG AGTTTGGCAC CTGTGAGGCC 
CAGGTGCTCC AGCTGTATAC AGAAGATGGG ACAGCTGTGG CCTCCATAGG GGATGGCCAG 
CGCTGTGGCC TCCTCTTGGA CAAAACCAAC TTCTACGCTG AACAGGGAGG CCAGGCTTCC 
GACCGAGGCT ACCTGGTGCT GGTGGGCCAG CAGGACGTGC TCTTCCCAGT GGCCCGGGCC 
CAGGTCTGCG GAGGCTTCAT CCTGCATGAG GTGGTGGCCC CGGAGCGCCT CAGGRTGGGG 
GACCAGGTGC AGCTGCACGT GGATGAGGCC TGGCGTCTGA GCTGCATGGA GAAGCACACG 
GCCATCCACC TGCTCAACTG GGCGCTGCGG CAGGTCCTGG GCCCCGGCAC AGAGCAGCGA 
GGCTCCCATC TCAATCCCGA GCGGCTGCGC TTCGACGTGG CCACGCAGGC CCCATTGACC 
CCAGAGCAGC TCCGGGCAGT GGAGGTCACT GTGCAGAAGG CCGTGGGGCG GGATGAGGCT 
GTGTACATGG AGGAGGTGGC CCTGGCACGC ACTGCCCACG TCCCCGGCCT GCGCTCCCTG 
GATGAGGTAT ACCCAGACCC TGTGCGGGTG GTATCCGTGG GGGTGCCCGT AGCCCATGCG 
CTGGACCCAG CCTCCCAAGC CGCACTGCAC ACCTCTGTGG AGCTATGCTG TGGGACGCAC 
CTGCTTCGCA CAGGGGCTAT AGGAGACCTG GTCATCATCG GAGAGCGCCA GCTCGTCAAG 
GGCATCACCC GCCTGCTGGC CGTCAATGGG GAGCAGGCCC AGCAGGCCCG AGAGGTGGGC 
CAGAGGCTGG CCCAGGAGGT GGAAGCAGCC GCAGCTCGGC TGAGTCAGGG CAGCCGGCAT 
GTGGCGGAGG CACATCGGCT GTCCAAGGAC ATGGGGCGAC TTACCGACGC CGTGGACACC 
GCCACGATGC CCCAGTGGCA GCGGCGGGAG CTTCAGGCCA CACTGAAGGC GCTGCAGCGG 
CGTGCCAACA CTGCCGTCCG GAAACTGGAA ATGGGGCAGG CTGCACAGAA GACCCAGGAG 
CTGCTCCAGC GGCACATGGA GGGGCCCCTG ATTGTGGACA CGGTTTCCAC GGAGTCCCTC 
TCTGTAAGCG TTGGGCGTGG GGTGGGCCCA GGGTGTGGKG AGGGGAAGGT CCCAGCGGGG 
AGGAAGCTGG GAGCTGCGAG GTCGCCATCT GCCCTCCCCC GCACAGATGC TGGTGAAGGT 
GGTCCGGCAG CTGTGCACGC AGGCGCCCGG CACGTCYGTG CTTCTCCTCA GCCCCCAGCC 
CCTGGGGAAG GTGCTGTGCG CCTGTCAGGT GGCCCAGAGT GCCACACCRG CCTTCACAGC 
AGAGGCCTGG GCGCTGGCCG TGTGCAGCCA CATGGGGGGC AAAGCCTGGG GCTCTCGAGT 
GGTGGCCCAG GGCACTGGAA GCACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019841736.1
CDS	104..2893
Translation	MNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGRHSDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPEDRLWVSYFGGDPKAGLGPDLESRDIWLSLGVPASRVLSFGPEENFWEMGDTGPCGPCTEIHYDLAGGAGAPQLVELWNLVFMQHNREADGSLQPLPQRHVDTGMGLERLVnullVLQGRLSTYDTDLFSPLLDAIHQGCGAPPYSGRIGAADEGRTDTAYRVVADHIRTLSVCIADGVSPGMSGnullPLVLRRILRRAVRFSTEVLRAPPGFLGSLVPVVVETLGDAYPELRRSSAQIASLVSEDEAAFLASLQRGRRVIERTLKRLGPSDVFPAEVAWSLSLSGNLGLPLDLVELMLEEKGVPLDTAGLERLAQEEAQHRALQAEPVQEQGLRLNVHALGELQRRGVPPTDDSPKYSYSLQPGGSYISALAEFGTCEAQVLQLYTEDGTAVASIGDGQRCGLLLDKTNFYAEQGGQASDRGYLVLVGQQDVLFPVARAQVCGGFILHEVVAPERLRnullGDQVQLHVDEAWRLSCMEKHTAIHLLNWALRQVLGPGTEQRGSHLNPERLRFDVATQAPLTPEQLRAVEVTVQKAVGRDEAVYMEEVALARTAHVPGLRSLDEVYPDPVRVVSVGVPVAHALDPASQAALHTSVELCCGTHLLRTGAIGDLVIIGERQLVKGITRLLAVNGEQAQQAREVGQRLAQEVEAAAARLSQGSRHVAEAHRLSKDMGRLTDAVDTATMPQWQRRELQATLKALQRRANTAVRKLEMGQAAQKTQELLQRHMEGPLIVDTVSTESLSVSVGRGVGPGCnullEGKVPAGRKLGAARSPSALPRTDAGEGGPAAVHAGARHVnullASPQPPAPGEGAVRLSGGPECHTnullLHSRGLGAGRVQPHGGQSLGLSSGGPGHWKHC

Target ORF information:

RefSeq Version	XM_019986177.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019986177.1

ORF Insert Sequence:

ATGAACCAGT TCAAGCCAAT CTTCCTGGGC ACTGTGGATC CACGAAGCGA GATGGCAGGC 
TTCCGGCGGG TAGCCAACAG CCAGAAATGT GTGAGGGCTG GAGGACGCCA CAGCGACCTG 
GAAGATGTGG GCCGAGACCT TTCCCATCAT ACCTTCTTCG AGATGCTGGG CAATTGGGCC 
TTTGGGGGTG AATATTTTAA GGAGGAGGCT TGCAGCATGG CCTGGGAGCT GCTGACTCAG 
GTCTATGGGA TCCCTGAGGA CAGGCTCTGG GTCTCCTACT TTGGTGGGGA CCCCAAGGCT 
GGGCTGGGCC CAGACTTGGA GAGCCGGGAC ATCTGGCTCA GCTTGGGGGT GCCTGCCAGC 
CGTGTGCTCT CCTTTGGGCC CGAGGAGAAC TTCTGGGAGA TGGGGGATAC TGGGCCTTGT 
GGGCCCTGCA CTGAGATACA CTATGACCTG GCCGGTGGGG CAGGGGCCCC CCAGCTGGTA 
GAGCTTTGGA ATCTGGTCTT CATGCAACAC AACAGAGAGG CAGACGGAAG CCTGCAGCCT 
CTGCCCCAGC GGCACGTGGA CACAGGAATG GGCCTAGAAA GGCTGGTGGC YGTGCTGCAA 
GGCAGGCTCT CCACCTATGA CACCGACCTG TTTTCCCCGC TGCTCGACGC CATACACCAG 
GGTTGCGGGG CACCCCCTTA CTCAGGCCGC ATTGGGGCAG CAGATGAGGG GCGCACAGAC 
ACAGCATACC GCGTGGTGGC TGATCACATC CGCACACTCA GTGTCTGCAT CGCTGACGGC 
GTCTCCCCTG GGATGTCAGG CGCYCCGCTG GTACTTCGTC GGATCCTCCG TCGAGCTGTG 
CGGTTCTCTA CTGAGGTGTT GCGGGCACCA CCTGGCTTCC TGGGCAGCCT GGTGCCTGTA 
GTGGTGGAGA CACTGGGAGA TGCTTACCCA GAATTGCGGA GGAGCTCAGC CCAGATAGCC 
AGCCTGGTGT CGGAGGATGA GGCAGCCTTC CTGGCCTCAC TGCAGCGTGG TCGGCGGGTC 
ATCGAGCGGA CCCTGAAGCG CCTGGGGCCT TCTGATGTGT TCCCAGCCGA AGTGGCCTGG 
TCCTTGTCAC TGTCTGGGAA CCTGGGGCTC CCCCTGGACC TGGTGGAGCT GATGCTGGAG 
GAGAAGGGGG TGCCGCTGGA CACTGCTGGA CTGGAGCGGC TGGCCCAGGA GGAGGCCCAG 
CACCGGGCAC TGCAGGCCGA GCCGGTTCAG GAGCAGGGAC TGCGACTCAA CGTCCACGCC 
CTGGGGGAGC TGCAGCGCCG AGGGGTGCCC CCAACCGATG ATAGCCCCAA GTACAGCTAC 
TCCCTGCAAC CAGGCGGCAG TTACATCTCG GCCCTGGCAG AGTTTGGCAC CTGTGAGGCC 
CAGGTGCTCC AGCTGTATAC AGAAGATGGG ACAGCTGTGG CCTCCATAGG GGATGGCCAG 
CGCTGTGGCC TCCTCTTGGA CAAAACCAAC TTCTACGCTG AACAGGGAGG CCAGGCTTCC 
GACCGAGGCT ACCTGGTGCT GGTGGGCCAG CAGGACGTGC TCTTCCCAGT GGCCCGGGCC 
CAGGTCTGCG GAGGCTTCAT CCTGCATGAG GTGGTGGCCC CGGAGCGCCT CAGGRTGGGG 
GACCAGGTGC AGCTGCACGT GGATGAGGCC TGGCGTCTGA GCTGCATGGA GAAGCACACG 
GCCATCCACC TGCTCAACTG GGCGCTGCGG CAGGTCCTGG GCCCCGGCAC AGAGCAGCGA 
GGCTCCCATC TCAATCCCGA GCGGCTGCGC TTCGACGTGG CCACGCAGGC CCCATTGACC 
CCAGAGCAGC TCCGGGCAGT GGAGGTCACT GTGCAGAAGG CCGTGGGGCG GGATGAGGCT 
GTGTACATGG AGGAGGTGGC CCTGGCACGC ACTGCCCACG TCCCCGGCCT GCGCTCCCTG 
GATGAGGTAT ACCCAGACCC TGTGCGGGTG GTATCCGTGG GGGTGCCCGT AGCCCATGCG 
CTGGACCCAG CCTCCCAAGC CGCACTGCAC ACCTCTGTGG AGCTATGCTG TGGGACGCAC 
CTGCTTCGCA CAGGGGCTAT AGGAGACCTG GTCATCATCG GAGAGCGCCA GCTCGTCAAG 
GGCATCACCC GCCTGCTGGC CGTCAATGGG GAGCAGGCCC AGCAGGCCCG AGAGGTGGGC 
CAGAGGCTGG CCCAGGAGGT GGAAGCAGCC GCAGCTCGGC TGAGTCAGGG CAGCCGGCAT 
GTGGCGGAGG CACATCGGCT GTCCAAGGAC ATGGGGCGAC TTACCGACGC CGTGGACACC 
GCCACGATGC CCCAGTGGCA GCGGCGGGAG CTTCAGGCCA CACTGAAGGC GCTGCAGCGG 
CGTGCCAACA CTGCCGTCCG GAAACTGGAA ATGGGGCAGG CTGCACAGAA GACCCAGGAG 
CTGCTCCAGC GGCACATGGA GGGGCCCCTG ATTGTGGACA CGGTTTCCAC GGAGTCCCTC 
TCTGTAAGCG TTGGGCGTGG GGTGGGCCCA GGGTGTGGKG AGGGGAAGGT CCCAGCGGGG 
AGGAAGCTGG GAGCTGCGAG GTCGCCATCT GCCCTCCCCC GCACAGATGC TGGTGAAGGT 
GGTCCGGCAG CTGTGCACGC AGGCGCCCGG CACGTCYGTG CTTCTCCTCA GCCCCCAGCC 
CCTGGGGAAG GTGCTGTGCG CCTGTCAGGT GGCCCAGAGT GCCACACCRG CCTTCACAGC 
AGAGGCCTGG GCGCTGGCCG TGTGCAGCCA CATGGGGGGC AAAGCCTGGG GCTCTCGAGT 
GGTGGCCCAG GGCACTGGAA GCACTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AARS2 ( XM_019986178.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019841737.1 Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo42194
Clone ID Related Accession (Same CDS sequence)	XM_019986178.1
Accession Version	XM_019986178.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	alanine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032672.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAGT TCAAGCCAAT CTTCCTGGGC ACTGTGGATC CACGAAGCGA GATGGCAGGC 
TTCCGGCGGG TAGCCAACAG CCAGAAATGT GTGAGGGCTG GAGGACGCCA CAGCGACCTG 
GAAGATGTGG GCCGAGACCT TTCCCATCAT ACCTTCTTCG AGATGCTGGG CAATTGGGCC 
TTTGGGGGTG AATATTTTAA GGAGGAGGCT TGCAGCATGG CCTGGGAGCT GCTGACTCAG 
GTCTATGGGA TCCCTGAGGA CAGGCTCTGG GTCTCCTACT TTGGTGGGGA CCCCAAGGCT 
GGGCTGGGCC CAGACTTGGA GAGCCGGGAC ATCTGGCTCA GCTTGGGGGT GCCTGCCAGC 
CGTGTGCTCT CCTTTGGGCC CGAGGAGAAC TTCTGGGAGA TGGGGGATAC TGGGCCTTGT 
GGGCCCTGCA CTGAGATACA CTATGACCTG GCCGGTGGGG CAGGGGCCCC CCAGCTGGTA 
GAGCTTTGGA ATCTGGTCTT CATGCAACAC AACAGAGAGG CAGACGGAAG CCTGCAGCCT 
CTGCCCCAGC GGCACGTGGA CACAGGAATG GGCCTAGAAA GGCTGGTGGC YGTGCTGCAA 
GGCAGGCTCT CCACCTATGA CACCGACCTG TTTTCCCCGC TGCTCGACGC CATACACCAG 
GGTTGCGGGG CACCCCCTTA CTCAGGCCGC ATTGGGGCAG CAGATGAGGG GCGCACAGAC 
ACAGCATACC GCGTGGTGGC TGATCACATC CGCACACTCA GTGTCTGCAT CGCTGACGGC 
GTCTCCCCTG GGATGTCAGG CGCYCCGCTG GTACTTCGTC GGATCCTCCG TCGAGCTGTG 
CGGTTCTCTA CTGAGGTGTT GCGGGCACCA CCTGGCTTCC TGGGCAGCCT GGTGCCTGTA 
GTGGTGGAGA CACTGGGAGA TGCTTACCCA GAATTGCGGA GGAGCTCAGC CCAGATAGCC 
AGCCTGGTGT CGGAGGATGA GGCAGCCTTC CTGGCCTCAC TGCAGCGTGG TCGGCGGGTC 
ATCGAGCGGA CCCTGAAGCG CCTGGGGCCT TCTGATGTGT TCCCAGCCGA AGTGGCCTGG 
TCCTTGTCAC TGTCTGGGAA CCTGGGGCTC CCCCTGGACC TGGTGGAGCT GATGCTGGAG 
GAGAAGGGGG TGCCGCTGGA CACTGCTGGA CTGGAGCGGC TGGCCCAGGA GGAGGCCCAG 
CACCGGGCAC TGCAGGCCGA GCCGGTTCAG GAGCAGGGAC TGCGACTCAA CGTCCACGCC 
CTGGGGGAGC TGCAGCGCCG AGGGGTGCCC CCAACCGATG ATAGCCCCAA GTACAGCTAC 
TCCCTGCAAC CAGGCGGCAG TTACAAGTTT GGCACCTGTG AGGCCCAGGT GCTCCAGCTG 
TATACAGAAG ATGGGACAGC TGTGGCCTCC ATAGGGGATG GCCAGCGCTG TGGCCTCCTC 
TTGGACAAAA CCAACTTCTA CGCTGAACAG GGAGGCCAGG CTTCCGACCG AGGCTACCTG 
GTGCTGGTGG GCCAGCAGGA CGTGCTCTTC CCAGTGGCCC GGGCCCAGGT CTGCGGAGGC 
TTCATCCTGC ATGAGGTGGT GGCCCCGGAG CGCCTCAGGR TGGGGGACCA GGTGCAGCTG 
CACGTGGATG AGGCCTGGCG TCTGAGCTGC ATGGAGAAGC ACACGGCCAT CCACCTGCTC 
AACTGGGCGC TGCGGCAGGT CCTGGGCCCC GGCACAGAGC AGCGAGGCTC CCATCTCAAT 
CCCGAGCGGC TGCGCTTCGA CGTGGCCACG CAGGCCCCAT TGACCCCAGA GCAGCTCCGG 
GCAGTGGAGG TCACTGTGCA GAAGGCCGTG GGGCGGGATG AGGCTGTGTA CATGGAGGAG 
GTGGCCCTGG CACGCACTGC CCACGTCCCC GGCCTGCGCT CCCTGGATGA GGTATACCCA 
GACCCTGTGC GGGTGGTATC CGTGGGGGTG CCCGTAGCCC ATGCGCTGGA CCCAGCCTCC 
CAAGCCGCAC TGCACACCTC TGTGGAGCTA TGCTGTGGGA CGCACCTGCT TCGCACAGGG 
GCTATAGGAG ACCTGGTCAT CATCGGAGAG CGCCAGCTCG TCAAGGGCAT CACCCGCCTG 
CTGGCCGTCA ATGGGGAGCA GGCCCAGCAG GCCCGAGAGG TGGGCCAGAG GCTGGCCCAG 
GAGGTGGAAG CAGCCGCAGC TCGGCTGAGT CAGGGCAGCC GGCATGTGGC GGAGGCACAT 
CGGCTGTCCA AGGACATGGG GCGACTTACC GACGCCGTGG ACACCGCCAC GATGCCCCAG 
TGGCAGCGGC GGGAGCTTCA GGCCACACTG AAGGCGCTGC AGCGGCGTGC CAACACTGCC 
GTCCGGAAAC TGGAAATGGG GCAGGCTGCA CAGAAGACCC AGGAGCTGCT CCAGCGGCAC 
ATGGAGGGGC CCCTGATTGT GGACACGGTT TCCACGGAGT CCCTCTCTAT GCTGGTGAAG 
GTGGTCCGGC AGCTGTGCAC GCAGGCGCCC GGCACGTCYG TGCTTCTCCT CAGCCCCCAG 
CCCCTGGGGA AGGTGCTGTG CGCCTGTCAG GTGGCCCAGA GTGCCACACC RGCCTTCACA 
GCAGAGGCCT GGGCGCTGGC CGTGTGCAGC CACATGGGGG GCAAAGCCTG GGGCTCTCGA 
GTGGTGGCCC AGGGCACTGG AAGCACTGCT GACCTGGAAG CTGCCCTCAG TGCAGCACGA 
GCCTATGCCC TCAACCAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019841737.1
CDS	104..2827
Translation	MNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGRHSDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPEDRLWVSYFGGDPKAGLGPDLESRDIWLSLGVPASRVLSFGPEENFWEMGDTGPCGPCTEIHYDLAGGAGAPQLVELWNLVFMQHNREADGSLQPLPQRHVDTGMGLERLVnullVLQGRLSTYDTDLFSPLLDAIHQGCGAPPYSGRIGAADEGRTDTAYRVVADHIRTLSVCIADGVSPGMSGnullPLVLRRILRRAVRFSTEVLRAPPGFLGSLVPVVVETLGDAYPELRRSSAQIASLVSEDEAAFLASLQRGRRVIERTLKRLGPSDVFPAEVAWSLSLSGNLGLPLDLVELMLEEKGVPLDTAGLERLAQEEAQHRALQAEPVQEQGLRLNVHALGELQRRGVPPTDDSPKYSYSLQPGGSYKFGTCEAQVLQLYTEDGTAVASIGDGQRCGLLLDKTNFYAEQGGQASDRGYLVLVGQQDVLFPVARAQVCGGFILHEVVAPERLRnullGDQVQLHVDEAWRLSCMEKHTAIHLLNWALRQVLGPGTEQRGSHLNPERLRFDVATQAPLTPEQLRAVEVTVQKAVGRDEAVYMEEVALARTAHVPGLRSLDEVYPDPVRVVSVGVPVAHALDPASQAALHTSVELCCGTHLLRTGAIGDLVIIGERQLVKGITRLLAVNGEQAQQAREVGQRLAQEVEAAAARLSQGSRHVAEAHRLSKDMGRLTDAVDTATMPQWQRRELQATLKALQRRANTAVRKLEMGQAAQKTQELLQRHMEGPLIVDTVSTESLSMLVKVVRQLCTQAPGTnullVLLLSPQPLGKVLCACQVAQSATnullAFTAEAWALAVCSHMGGKAWGSRVVAQGTGSTADLEAALSAARAYALNQL

Target ORF information:

RefSeq Version	XM_019986178.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus alanyl-tRNA synthetase 2, mitochondrial (AARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019986178.1

ORF Insert Sequence:

ATGAACCAGT TCAAGCCAAT CTTCCTGGGC ACTGTGGATC CACGAAGCGA GATGGCAGGC 
TTCCGGCGGG TAGCCAACAG CCAGAAATGT GTGAGGGCTG GAGGACGCCA CAGCGACCTG 
GAAGATGTGG GCCGAGACCT TTCCCATCAT ACCTTCTTCG AGATGCTGGG CAATTGGGCC 
TTTGGGGGTG AATATTTTAA GGAGGAGGCT TGCAGCATGG CCTGGGAGCT GCTGACTCAG 
GTCTATGGGA TCCCTGAGGA CAGGCTCTGG GTCTCCTACT TTGGTGGGGA CCCCAAGGCT 
GGGCTGGGCC CAGACTTGGA GAGCCGGGAC ATCTGGCTCA GCTTGGGGGT GCCTGCCAGC 
CGTGTGCTCT CCTTTGGGCC CGAGGAGAAC TTCTGGGAGA TGGGGGATAC TGGGCCTTGT 
GGGCCCTGCA CTGAGATACA CTATGACCTG GCCGGTGGGG CAGGGGCCCC CCAGCTGGTA 
GAGCTTTGGA ATCTGGTCTT CATGCAACAC AACAGAGAGG CAGACGGAAG CCTGCAGCCT 
CTGCCCCAGC GGCACGTGGA CACAGGAATG GGCCTAGAAA GGCTGGTGGC YGTGCTGCAA 
GGCAGGCTCT CCACCTATGA CACCGACCTG TTTTCCCCGC TGCTCGACGC CATACACCAG 
GGTTGCGGGG CACCCCCTTA CTCAGGCCGC ATTGGGGCAG CAGATGAGGG GCGCACAGAC 
ACAGCATACC GCGTGGTGGC TGATCACATC CGCACACTCA GTGTCTGCAT CGCTGACGGC 
GTCTCCCCTG GGATGTCAGG CGCYCCGCTG GTACTTCGTC GGATCCTCCG TCGAGCTGTG 
CGGTTCTCTA CTGAGGTGTT GCGGGCACCA CCTGGCTTCC TGGGCAGCCT GGTGCCTGTA 
GTGGTGGAGA CACTGGGAGA TGCTTACCCA GAATTGCGGA GGAGCTCAGC CCAGATAGCC 
AGCCTGGTGT CGGAGGATGA GGCAGCCTTC CTGGCCTCAC TGCAGCGTGG TCGGCGGGTC 
ATCGAGCGGA CCCTGAAGCG CCTGGGGCCT TCTGATGTGT TCCCAGCCGA AGTGGCCTGG 
TCCTTGTCAC TGTCTGGGAA CCTGGGGCTC CCCCTGGACC TGGTGGAGCT GATGCTGGAG 
GAGAAGGGGG TGCCGCTGGA CACTGCTGGA CTGGAGCGGC TGGCCCAGGA GGAGGCCCAG 
CACCGGGCAC TGCAGGCCGA GCCGGTTCAG GAGCAGGGAC TGCGACTCAA CGTCCACGCC 
CTGGGGGAGC TGCAGCGCCG AGGGGTGCCC CCAACCGATG ATAGCCCCAA GTACAGCTAC 
TCCCTGCAAC CAGGCGGCAG TTACAAGTTT GGCACCTGTG AGGCCCAGGT GCTCCAGCTG 
TATACAGAAG ATGGGACAGC TGTGGCCTCC ATAGGGGATG GCCAGCGCTG TGGCCTCCTC 
TTGGACAAAA CCAACTTCTA CGCTGAACAG GGAGGCCAGG CTTCCGACCG AGGCTACCTG 
GTGCTGGTGG GCCAGCAGGA CGTGCTCTTC CCAGTGGCCC GGGCCCAGGT CTGCGGAGGC 
TTCATCCTGC ATGAGGTGGT GGCCCCGGAG CGCCTCAGGR TGGGGGACCA GGTGCAGCTG 
CACGTGGATG AGGCCTGGCG TCTGAGCTGC ATGGAGAAGC ACACGGCCAT CCACCTGCTC 
AACTGGGCGC TGCGGCAGGT CCTGGGCCCC GGCACAGAGC AGCGAGGCTC CCATCTCAAT 
CCCGAGCGGC TGCGCTTCGA CGTGGCCACG CAGGCCCCAT TGACCCCAGA GCAGCTCCGG 
GCAGTGGAGG TCACTGTGCA GAAGGCCGTG GGGCGGGATG AGGCTGTGTA CATGGAGGAG 
GTGGCCCTGG CACGCACTGC CCACGTCCCC GGCCTGCGCT CCCTGGATGA GGTATACCCA 
GACCCTGTGC GGGTGGTATC CGTGGGGGTG CCCGTAGCCC ATGCGCTGGA CCCAGCCTCC 
CAAGCCGCAC TGCACACCTC TGTGGAGCTA TGCTGTGGGA CGCACCTGCT TCGCACAGGG 
GCTATAGGAG ACCTGGTCAT CATCGGAGAG CGCCAGCTCG TCAAGGGCAT CACCCGCCTG 
CTGGCCGTCA ATGGGGAGCA GGCCCAGCAG GCCCGAGAGG TGGGCCAGAG GCTGGCCCAG 
GAGGTGGAAG CAGCCGCAGC TCGGCTGAGT CAGGGCAGCC GGCATGTGGC GGAGGCACAT 
CGGCTGTCCA AGGACATGGG GCGACTTACC GACGCCGTGG ACACCGCCAC GATGCCCCAG 
TGGCAGCGGC GGGAGCTTCA GGCCACACTG AAGGCGCTGC AGCGGCGTGC CAACACTGCC 
GTCCGGAAAC TGGAAATGGG GCAGGCTGCA CAGAAGACCC AGGAGCTGCT CCAGCGGCAC 
ATGGAGGGGC CCCTGATTGT GGACACGGTT TCCACGGAGT CCCTCTCTAT GCTGGTGAAG 
GTGGTCCGGC AGCTGTGCAC GCAGGCGCCC GGCACGTCYG TGCTTCTCCT CAGCCCCCAG 
CCCCTGGGGA AGGTGCTGTG CGCCTGTCAG GTGGCCCAGA GTGCCACACC RGCCTTCACA 
GCAGAGGCCT GGGCGCTGGC CGTGTGCAGC CACATGGGGG GCAAAGCCTG GGGCTCTCGA 
GTGGTGGCCC AGGGCACTGG AAGCACTGCT GACCTGGAAG CTGCCCTCAG TGCAGCACGA 
GCCTATGCCC TCAACCAGCT CTGA