LOC109575111 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109575111
Entrez Gene ID	109575111
Full Name	ubiquitin-protein ligase E3A
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019983169.1	XP_019838728.1	ubiquitin-protein ligase E3A

Pathways from BioSystems
Gene Ontology

The following LOC109575111 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109575111 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo39950	XM_019983169.1 Latest version!	Bos indicus ubiquitin-protein ligase E3A (LOC109575111), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109575111 ( XM_019983169.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019838728.1 Bos indicus ubiquitin-protein ligase E3A (LOC109575111), mRNA.

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CloneID	OBo39950
Clone ID Related Accession (Same CDS sequence)	XM_019983169.1
Accession Version	XM_019983169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	ubiquitin-protein ligase E3A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032670.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT 
GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT 
GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT 
AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT 
GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT 
CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA 
GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA 
GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA 
AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT 
GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT 
CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA 
TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC 
CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG 
GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT 
CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT 
GCAAATGTAG TAGGAGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC 
CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG 
AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA 
AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG 
GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT 
CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC 
ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT 
CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA 
GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA 
GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG 
GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG 
TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT 
TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG 
CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG 
AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC 
CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT 
GATAAAATTC CATTACCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019838728.1
CDS	171..2291
Translation	MATACKRSSGEPQSDDIEASRMKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFRRMDNNAAAIKALELYKINAKLCDPHPSKKGASSAYLENSKGASNNCSDIKMNKKDGQGARDDFKDVTYLTEEMVYEILELCREREDYSPLIRVIGRVFSSAEALVQSFRKVKQHTKEELKSLQGKDEDKDEDEKEKAACSAAAMEEDSEASSSRISDSSQGDNNLQKLGPDDVSVDIEAIRRVYTRLLSNEKIETAFLNALVYLSPNVECDLTYHNVYSRDPNYLNLFIIVMENRNLHSPEYLEMALPLFCKAMSKLPLAAQGKLVRLWSKYSADQIRRMMETFQQLITYKVISTDFNNRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPLPESSELTLQELLGEERRNKKGPRVDPLETELAVKTLDCRKPLIPFEEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFLDLGDSHPVLYQSLKDLLEYEGNVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPLP

Target ORF information:

RefSeq Version	XM_019983169.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus ubiquitin-protein ligase E3A (LOC109575111), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019983169.1

ORF Insert Sequence:

ATGGCCACAG CTTGTAAAAG ATCATCAGGA GAACCTCAAT CTGACGACAT TGAAGCTAGC 
CGAATGAAGC GAGCAGCTGC AAAACATCTA ATAGAACGCT ACTACCACCA GTTAACTGAG 
GGCTGTGGAA ATGAGGCCTG CACGAATGAG TTTTGTGCTT CCTGTCCAAC TTTTCGTCGT 
ATGGATAACA ATGCAGCAGC TATTAAAGCC CTCGAGCTTT ATAAGATTAA TGCAAAACTC 
TGTGATCCTC ATCCCTCCAA GAAAGGAGCA AGCTCAGCTT ACCTTGAAAA CTCTAAAGGT 
GCCTCTAACA ACTGCTCTGA CATAAAAATG AACAAGAAGG ACGGGCAAGG AGCAAGAGAT 
GATTTTAAAG ATGTGACTTA CCTAACCGAA GAGATGGTGT ATGAAATTCT TGAATTATGT 
AGAGAGAGAG AGGATTATTC CCCTTTAATT CGTGTTATTG GAAGAGTTTT TTCTAGTGCT 
GAAGCATTGG TACAAAGCTT TCGGAAAGTT AAGCAACACA CCAAGGAAGA ACTGAAATCT 
CTTCAAGGAA AGGATGAAGA TAAAGATGAA GACGAAAAGG AAAAGGCTGC ATGTTCCGCA 
GCAGCTATGG AGGAGGATTC AGAAGCATCT TCCTCAAGGA TAAGTGATAG CTCACAAGGA 
GATAACAACT TACAAAAGTT AGGCCCTGAT GATGTGTCTG TGGATATTGA GGCTATTAGA 
AGGGTCTACA CCAGATTGCT CTCTAATGAA AAAATAGAAA CTGCTTTTCT CAATGCACTT 
GTGTATTTGT CACCTAATGT GGAATGTGAC TTGACGTATC ACAATGTATA CTCCCGAGAT 
CCTAATTATC TGAATTTATT CATTATTGTC ATGGAGAACA GAAATCTCCA CAGTCCTGAA 
TATCTGGAAA TGGCTTTGCC ACTATTTTGC AAAGCAATGA GTAAGCTACC CCTTGCAGCC 
CAAGGAAAAC TGGTCAGACT GTGGTCTAAA TACAGTGCAG ACCAGATTCG GAGAATGATG 
GAAACATTTC AGCAGCTTAT TACATACAAA GTCATAAGCA CTGACTTTAA CAATCGAAAT 
CTAGTGAACG ATGATGATGC TATTGTTGCT GCTTCCAAGT GCTTGAAAAT GGTTTACTAT 
GCAAATGTAG TAGGAGGGGA GGTAGACACA AATCATAATG AAGAAGATGA TGAAGAGCCC 
CTTCCTGAGT CCAGTGAATT GACACTTCAG GAGTTATTGG GAGAAGAAAG AAGGAACAAG 
AAAGGTCCTC GAGTGGACCC ACTGGAAACT GAACTTGCTG TTAAAACTCT GGATTGTCGA 
AAACCACTTA TCCCTTTTGA AGAGTTTATT AATGAACCAC TGAATGATGT TCTAGAAATG 
GATAAAGATT ATACTTTTTT CAAAGTAGAA ACAGAGAACA AGTTCTCTTT TATGACATGT 
CCCTTTATAC TGAATGCTGT CACAAAGAAT TTGGGATTAT ATTATGACAA TAGAATTCGC 
ATGTACAGTG AACGAAGAAT TACTGTTCTC TACAGCTTAG TTCAAGGACA GCAGTTGAAT 
CCATATTTGA GACTCAAAGT CAGACGTGAC CATATCATAG ATGATGCACT TGTCCGGCTA 
GAGATGATCG CTATGGAAAA TCCTGCAGAC TTGAAGAAAC AGTTGTATGT GGAATTTGAA 
GGAGAACAAG GAGTTGATGA GGGAGGTGTT TCCAAAGAAT TTTTTCAGCT GGTTGTGGAG 
GAAATCTTCA ATCCAGATAT TGGTATGTTC ACATATGATG AATCTACAAA GTTGTTTTGG 
TTTAATCCAT CCTCTTTTGA AACTGAGGGT CAGTTCACTC TGATTGGCAT AGTACTGGGT 
TTGGCTATTT ACAATAACTG TATACTGGAT GTACATTTTC CCATGGTTGT CTACAGGAAG 
CTAATGGGGA AAAAAGGAAC TTTTCTTGAC TTGGGAGACT CTCATCCTGT TCTGTATCAG 
AGTTTAAAAG ACTTACTGGA GTATGAAGGG AATGTGGAAG ATGATATGAT GATCACTTTC 
CAGATATCTC AGACAGATCT TTTTGGTAAT CCGATGATGT ATGATCTAAA GGAAAATGGT 
GATAAAATTC CATTACCATG A