LOC100748791 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100748791
Entrez Gene ID	100748791
Full Name	putative glutamate synthase [NADPH]
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012392329.1	XP_012247752.1	putative glutamate synthase [NADPH]

Pathways from BioSystems
Gene Ontology

The following LOC100748791 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100748791 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo110621	XM_012392329.1 Latest version!	Bombus impatiens putative glutamate synthase [NADPH] (LOC100748791), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100748791 ( XM_012392329.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012247752.1 Bombus impatiens putative glutamate synthase [NADPH] (LOC100748791), mRNA.

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CloneID	OBo110621
Clone ID Related Accession (Same CDS sequence)	XM_012392329.1
Accession Version	XM_012392329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	putative glutamate synthase [NADPH]
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177767.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 100% of CDS bases ##RefSeq-Attributes-END##

ATGAATCAGA AAGTATATCA ATTTATCGAT CTAGAGCGTG TCGATCCGCC TAAAACAGCA 
CTAGCGGTGA GAAAAGTAGA ATTTGTTGAG ATTTATGAAG ATTTTGTTAC TAAACAAGCA 
CAAACTCAAG CTGAACGCTG CTTAGCCTGT GGCAACCCTT ACTGCGAATG GAAATGTCCT 
TTACATAACT ATATTCCTAA TTGGTTAAAG TTAGTTAAAG AAGGACGAGT GTTAGAAGCA 
GCTGAACTTT GCCATCAAAC TAACAGTTTA CCTGAAGTGT GTGGTCGCGT TTGTCCACAA 
GATCGCTTAT GTGAAGGTTC TTGTACATTG AGCGCCGAAT TTGGTGCGGT GACGATTGGT 
CATATTGAGC GTTATATTAC CGACCAAGCG TTCCAATTGG GTTGGAAACC CAATTTAAAT 
GATGTGGAGC ATTCAGGTAA GCGTGTGGCA ATTGTTGGTG CTGGTCCTGC TGGCTTAGCA 
TGTGCTGATA TATTAGTACG TCATGGTATT TCACCAGTTG TATTTGATCG CCATCCTGAA 
ATTGGGGGAT TATTAACCTT TGGTATTCCC TCATTTAAAT TAGATAAAGC AGTGATGACC 
CATCGGCGTC AGCTCTTTAC TGAAATGGGG ATTGAATTTA GGCTAAATAC TGAAGTAGGT 
AAAGATGTTG ATTTTCAATC ACTTCTCGCT GAGTTTGATG CCGTGTTTAT TGCTGTTGGA 
ACCTATCAAT CGATAAAAGG GGGATTTGAT AATGAACACG CAGCGGGAGT TTACGCAGCA 
TTACCCTATT TAATTGCCAA TACGAAACAT ACTTTAGGGT TAACATCATC TGATTATGTC 
GATATGCAAG GTAAGCGTGT CGTGGTGTTA GGGGGTGGCG ATACCGCGAT GGACTGCGTC 
CGTACGGCGA TTCGTCAAGG TGCCGCAGAA GTGACGTGCG CATATCGACG CGATGAAAGT 
AATATGCCAG GTTCAAAACG TGAAGTCATC AATGCACGGG AAGAGGGCGC TAAATTCTTA 
TTTAATTTAC AACCAATTGA TTTAGTATTA GATCAGCAAA ATCAGGTTAA TGGGATCCGC 
ATGGTCAAGA CTTTTTTAGG TGACGCTGAT AATCACGGTC GCCGTTATCC TGTTGTGGTT 
GAAAATTCAG AGCATATTTT GCCTGCTGAT GCTGTCATTA TGGCATTTGG CTTTAAGCCG 
CACGCCATGC CTTGGTTGGC ACCATTTGAT GTCAATGTTG ATGCTCAAGG ACGAATTATT 
GCCCCTGCTG ATAGCAGCTA TCAGACCTCC AATCCTAAAA TTTTCGCTGG TGGCGATGTG 
GTTCGTGGTT CTGATTTAGT GGTAACGGCC ATCGCCGAAG GTCGAAAAGC CGCTGAAGGT 
ATCATCGCCT TTTTGGATAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012247752.1
CDS	1..1404
Translation	MNQKVYQFIDLERVDPPKTALAVRKVEFVEIYEDFVTKQAQTQAERCLACGNPYCEWKCPLHNYIPNWLKLVKEGRVLEAAELCHQTNSLPEVCGRVCPQDRLCEGSCTLSAEFGAVTIGHIERYITDQAFQLGWKPNLNDVEHSGKRVAIVGAGPAGLACADILVRHGISPVVFDRHPEIGGLLTFGIPSFKLDKAVMTHRRQLFTEMGIEFRLNTEVGKDVDFQSLLAEFDAVFIAVGTYQSIKGGFDNEHAAGVYAALPYLIANTKHTLGLTSSDYVDMQGKRVVVLGGGDTAMDCVRTAIRQGAAEVTCAYRRDESNMPGSKREVINAREEGAKFLFNLQPIDLVLDQQNQVNGIRMVKTFLGDADNHGRRYPVVVENSEHILPADAVIMAFGFKPHAMPWLAPFDVNVDAQGRIIAPADSSYQTSNPKIFAGGDVVRGSDLVVTAIAEGRKAAEGIIAFLDI

Target ORF information:

RefSeq Version	XM_012392329.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens putative glutamate synthase [NADPH] (LOC100748791), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012392329.1

ORF Insert Sequence:

ATGAATCAGA AAGTATATCA ATTTATCGAT CTAGAGCGTG TCGATCCGCC TAAAACAGCA 
CTAGCGGTGA GAAAAGTAGA ATTTGTTGAG ATTTATGAAG ATTTTGTTAC TAAACAAGCA 
CAAACTCAAG CTGAACGCTG CTTAGCCTGT GGCAACCCTT ACTGCGAATG GAAATGTCCT 
TTACATAACT ATATTCCTAA TTGGTTAAAG TTAGTTAAAG AAGGACGAGT GTTAGAAGCA 
GCTGAACTTT GCCATCAAAC TAACAGTTTA CCTGAAGTGT GTGGTCGCGT TTGTCCACAA 
GATCGCTTAT GTGAAGGTTC TTGTACATTG AGCGCCGAAT TTGGTGCGGT GACGATTGGT 
CATATTGAGC GTTATATTAC CGACCAAGCG TTCCAATTGG GTTGGAAACC CAATTTAAAT 
GATGTGGAGC ATTCAGGTAA GCGTGTGGCA ATTGTTGGTG CTGGTCCTGC TGGCTTAGCA 
TGTGCTGATA TATTAGTACG TCATGGTATT TCACCAGTTG TATTTGATCG CCATCCTGAA 
ATTGGGGGAT TATTAACCTT TGGTATTCCC TCATTTAAAT TAGATAAAGC AGTGATGACC 
CATCGGCGTC AGCTCTTTAC TGAAATGGGG ATTGAATTTA GGCTAAATAC TGAAGTAGGT 
AAAGATGTTG ATTTTCAATC ACTTCTCGCT GAGTTTGATG CCGTGTTTAT TGCTGTTGGA 
ACCTATCAAT CGATAAAAGG GGGATTTGAT AATGAACACG CAGCGGGAGT TTACGCAGCA 
TTACCCTATT TAATTGCCAA TACGAAACAT ACTTTAGGGT TAACATCATC TGATTATGTC 
GATATGCAAG GTAAGCGTGT CGTGGTGTTA GGGGGTGGCG ATACCGCGAT GGACTGCGTC 
CGTACGGCGA TTCGTCAAGG TGCCGCAGAA GTGACGTGCG CATATCGACG CGATGAAAGT 
AATATGCCAG GTTCAAAACG TGAAGTCATC AATGCACGGG AAGAGGGCGC TAAATTCTTA 
TTTAATTTAC AACCAATTGA TTTAGTATTA GATCAGCAAA ATCAGGTTAA TGGGATCCGC 
ATGGTCAAGA CTTTTTTAGG TGACGCTGAT AATCACGGTC GCCGTTATCC TGTTGTGGTT 
GAAAATTCAG AGCATATTTT GCCTGCTGAT GCTGTCATTA TGGCATTTGG CTTTAAGCCG 
CACGCCATGC CTTGGTTGGC ACCATTTGAT GTCAATGTTG ATGCTCAAGG ACGAATTATT 
GCCCCTGCTG ATAGCAGCTA TCAGACCTCC AATCCTAAAA TTTTCGCTGG TGGCGATGTG 
GTTCGTGGTT CTGATTTAGT GGTAACGGCC ATCGCCGAAG GTCGAAAAGC CGCTGAAGGT 
ATCATCGCCT TTTTGGATAT TTAA