tmem74 cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmem74
Entrez Gene ID	110171442
Full Name	transmembrane protein 74
Gene Type	protein-coding
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020937151.1	XP_020792810.1	transmembrane protein 74

Pathways from BioSystems
Gene Ontology

The following tmem74 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmem74 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo81695	XM_020937151.1 Latest version!	Boleophthalmus pectinirostris transmembrane protein 74 (tmem74), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmem74 ( XM_020937151.1 ) cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper) -> XP_020792810.1 Boleophthalmus pectinirostris transmembrane protein 74 (tmem74), mRNA.

Price & Availability↑

CloneID	OBo81695
Clone ID Related Accession (Same CDS sequence)	XM_020937151.1
Accession Version	XM_020937151.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Product	transmembrane protein 74
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018359358.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Boleophthalmus pectinirostris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 22% of CDS bases ##RefSeq-Attributes-END##

ATGGCTACTA CAACCACCTC TACCACCAAT ACAACAGCAG CACCAGTGCC GGAGATACTT 
CCCCCACGTG ATGAGGGGAA ACAACCTGAT CCTTCCCACG TCCTTGAGCA TATGTCAGGC 
CCGAAGCTGG TCTGTAAGCC TGCCGTGTCA ACAGGAGCTA TCCCAGGGGA GAGCTCATGC 
AGCGTGCCCC GCAGTTGTAA CCACGTCCGG TGTCATTCAC TTACAAGGAC GAGGAAGCCT 
ACAGCAGGAG GAGTTGAGGA GAAAGTAAGA GTTTGTTGTG ATGAGGAGCT TGAGACTTCA 
TTCACGTATG TTGATGAGAA TGTAAATTTG CGAGTGACCA GCCCAGAAAC GAGTTGTAAA 
AGCACACATA GGACCACACG TAACGGCGAA CCATGTTCAG AAACACAGCC AGACTTCTCC 
TGTATGTCTG AGGATGATTT GTCATTTGGA GACAGTTCTG GGACTTCCAT AGACTACGGT 
TTTATAAGTG CGGTCACCTT TTTGGTGACT GGCATCTCTC TGGTGATCAT ATCCTATGTA 
GTTCCGAGGG ATGTTGAAGT AGATCGGGAC ACAGTATCAG CCAGAGAGAT GGAGAGGTTG 
GAGACAGAGA GTGCACGGAT AGGAGCCCAC CTGGACCGCT GTGTGATAGC CGGGTTATGT 
CTGCTGACTC TAGGGGGAGT GGTGCTGTCC ACGCTACTCA TGATCTCCAT GTGGAAAGGG 
GAGATGTACA GGCGGAAAGT CATAGCATAC TCCAAACGTA CAGCCAAACT TTACGGATCC 
ATCAGCCTCA AGACCAGATC CAGTCCTAGT CACTCATCTG TGCAAGTGTC CATAGATGAA 
CTGGATGAAA CTATAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020792810.1
CDS	1..861
Translation	MATTTTSTTNTTAAPVPEILPPRDEGKQPDPSHVLEHMSGPKLVCKPAVSTGAIPGESSCSVPRSCNHVRCHSLTRTRKPTAGGVEEKVRVCCDEELETSFTYVDENVNLRVTSPETSCKSTHRTTRNGEPCSETQPDFSCMSEDDLSFGDSSGTSIDYGFISAVTFLVTGISLVIISYVVPRDVEVDRDTVSAREMERLETESARIGAHLDRCVIAGLCLLTLGGVVLSTLLMISMWKGEMYRRKVIAYSKRTAKLYGSISLKTRSSPSHSSVQVSIDELDETIA

Target ORF information:

RefSeq Version	XM_020937151.1
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Definition	Boleophthalmus pectinirostris transmembrane protein 74 (tmem74), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020937151.1

ORF Insert Sequence:

ATGGCTACTA CAACCACCTC TACCACCAAT ACAACAGCAG CACCAGTGCC GGAGATACTT 
CCCCCACGTG ATGAGGGGAA ACAACCTGAT CCTTCCCACG TCCTTGAGCA TATGTCAGGC 
CCGAAGCTGG TCTGTAAGCC TGCCGTGTCA ACAGGAGCTA TCCCAGGGGA GAGCTCATGC 
AGCGTGCCCC GCAGTTGTAA CCACGTCCGG TGTCATTCAC TTACAAGGAC GAGGAAGCCT 
ACAGCAGGAG GAGTTGAGGA GAAAGTAAGA GTTTGTTGTG ATGAGGAGCT TGAGACTTCA 
TTCACGTATG TTGATGAGAA TGTAAATTTG CGAGTGACCA GCCCAGAAAC GAGTTGTAAA 
AGCACACATA GGACCACACG TAACGGCGAA CCATGTTCAG AAACACAGCC AGACTTCTCC 
TGTATGTCTG AGGATGATTT GTCATTTGGA GACAGTTCTG GGACTTCCAT AGACTACGGT 
TTTATAAGTG CGGTCACCTT TTTGGTGACT GGCATCTCTC TGGTGATCAT ATCCTATGTA 
GTTCCGAGGG ATGTTGAAGT AGATCGGGAC ACAGTATCAG CCAGAGAGAT GGAGAGGTTG 
GAGACAGAGA GTGCACGGAT AGGAGCCCAC CTGGACCGCT GTGTGATAGC CGGGTTATGT 
CTGCTGACTC TAGGGGGAGT GGTGCTGTCC ACGCTACTCA TGATCTCCAT GTGGAAAGGG 
GAGATGTACA GGCGGAAAGT CATAGCATAC TCCAAACGTA CAGCCAAACT TTACGGATCC 
ATCAGCCTCA AGACCAGATC CAGTCCTAGT CACTCATCTG TGCAAGTGTC CATAGATGAA 
CTGGATGAAA CTATAGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.