ganab cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper)

Gene
Clones
gRNAs

Summary

Gene Symbol	ganab
Entrez Gene ID	110158892
Full Name	glucosidase II alpha subunit
Gene Type	protein-coding
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020923440.1	XP_020779099.1	neutral alpha-glucosidase AB isoform X2
XM_020923439.1	XP_020779098.1	neutral alpha-glucosidase AB isoform X1

Pathways from BioSystems
Gene Ontology

The following ganab gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ganab cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo91115	XM_020923439.1 Latest version!	Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo91116	XM_020923440.1 Latest version!	Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ganab ( XM_020923439.1 ) cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper) -> XP_020779098.1 Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo91115
Clone ID Related Accession (Same CDS sequence)	XM_020923439.1
Accession Version	XM_020923439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Product	neutral alpha-glucosidase AB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018354349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Boleophthalmus pectinirostris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCACTGAAAG GATGGCGCCT GTCCTAGCAT TTATACTGGT TCTCTGTCTT 
GGGGAGACAT GGGCTGTGGA CAGGGGCAAC TTTAAAACCT GTGACCAGAG TGCCTTCTGC 
AAGCGCCAGC GTGCATTGAA GCCAGGGGAA TCTCCTTATC GAGCCCTGCT AGAGACGATG 
GAGCTGACTA ACACTAGACT AACACTTCAG CTCATCAATG ATAACAATAA GGTGCGTCTG 
TTGCTTGAGC TCTACCGTCT CCAGGGAAAC ATGACAAGGG TGAAGATTAA TGAGCTGAAG 
CCTCTGAAGC CGCGCTATGA GGTCCCAGAT GTCCTCATCA AGGAGCCACC CACTGAGCCT 
CTGGCACTTT TATCCCAAGA TGAGAATGGG GTGGTACTGT CCCTGGGTGC AGAGTCCCAG 
AGGGTTATTG TTAGCGCACG TCCCTTTCGT TTGGATATCA TGGAGGGGCA TGACGTGCTG 
ATGTCACTGA ACTCGCGTGG ACTCTTGGCT TTTGAGCACT TGAGGATGCG CAAGGACACT 
TTCTCCTATA AAGTAACTAG CACAGTGGCT AGTGTGTGGG ATAATGTCAA GAGGGTTTTC 
TCTAGTCAAG CAGAGCCAGA TGCAGAGAAG AAAGAAGATG TTGATCCCGC TAACCAGCCT 
GAGGCATCAA AAGAAGAGGA TGAGAAAATA GAAGAAGGCA TGTGGGAAGA AACGTTCAAG 
TCTCACTCAG ACACCAAACC AAATGGTCCT TCAGCTATAA GTTTAGACTT TTCTTTGCCT 
GGAGTGGAAC ATGTGTATGG CATCCCCGAA CATGCAGATA CTCTTAAACT TAAAACAACT 
GAAAATGGAG ATCCATATCG GCTGTATAAC TTGGATGTGT TCCAGTATGA ACTTTATAAT 
CCTATGGCTC TTTATGGAGC GGTCCCAGTT ATGTTGGCCC ACAACACACA GCGGACGACT 
GGTATTTTCT GGCTGAATGC TGCAGAGACG TGGGTGGATA TCAGCTCTAA CACAGCTGGA 
AAAACTGTTT TTGGGAAGAT GCTAGATTAT GTGCAGGGAT CAAGTGAGAA GCCACAAACT 
GATGTCCGTT GGATCTCTGA AAGTGGCATT ATTGATGTAT TCATAATGCT TGGACCAACA 
CCTAAAGACG TGTTTTCACA ATATGCCTCA CTCACAGGCA CACAGTCTTT TCCACCTTTT 
GCTGCTCTGG GCTACCACCA GTGTCGATGG AACTATAATG ATCAGGAGGA TGTAAAGAAT 
GTTGATGCTG GCTTTGACGA ACATGACATC CCCTATGACT TTATTTGGCT TGATATTGAG 
CACACTGATG GAAAGCGCTA CTTCACCTGG GATCCTCACA AGTTTGCCAG TCCTAAGGAG 
ATGCTGCAGG GTATCAAGGA GAAGAAACGC AAGATGGTGG CCATTGTTGA CCCACATATC 
AAAGTTGACA GCAATTACAA GATCCATAAC GAAATCCGGT CAAAAGGTTT TTATGTTAAG 
AACAAGGATG GTGGAGACTA TGAGGGCTGG TGCTGGCCAG GTAGTGCTGG TTATCCAGAT 
TTCACCCGTG CAGACATGAG GGCTTGGTGG GCCAGCATGT TTGCCTATGA TCAATATGAA 
GGTAGTATGG AAAACTTGTA CACATGGAAT GACATGAATG AGCCATCGGT TTTTAATGGA 
CCAGAGGTCA CCATGCACAA AGATGCCATG CACCAAGACT GGGAGCATCG TGATGTTCAT 
AACTTATATG GGCTTTATGT GCAAATGGCC ACAGCTGAAG GTCAGATCCA GAGATCTGGA 
GGTGTAGAGC GTCCATTCGT CCTTACCAGG GCCTTTTTTG CGGGATCACA GCGCTTTGGT 
GCTGTGTGGA CTGGCGACAA TGCCGCGGAG TGGGACCATC TTAAGATCTC TATCCCAATG 
TGCTTGAGCC TTGGCTTGGT TGGAATTTCT TTTTGTGGAG CTGACGTGGG AGGCTTTTTC 
AAGTCTCCGA GCTCTGAGCT TCTGGTGCGT TGGTACCAAA CCGGAGCCTA CCAGCCATTC 
TTCCGGGCAC ATGCTCACCT GGACACGCCA CGCCGGGAGC CATGGCTCTT TGGTCCTGAG 
AACACTGCCC TGATCCGTGA CGCAGTGCGC CAGCGCTACA CTCTGCTGCC CTACTGGTAC 
CAACTGTTCT ACCATGCATA CCTAACTGGA GAGCCTGTCA TGAGACCATT GTGGATAGAG 
TATCCTCAAG ATCCTGCTAC TTTTGCCATT GATGATGAGT TTCTCATTGG AAAAGACTTG 
TTGGTGCATC CTGTGACCGA GGAGGGATCT AGGGGAGTCA CTGCATACCT GCCAGGGAAA 
GATGATGTCT GGTATGATGT CCAAACGTTC CAGAAGCACA ACGGGGCCCA AAACCTTTAC 
ATTCCTGTGA CAATGAGTAG TATTCCAGTT TTCCAGCGTG GTGGTTCCAT TATTCCAAGG 
AAGCTCCGTG TTCGCAGATC ATCAACTTGT ATGGAAAATG ATCCTTACAC TTTATACATT 
GCCTTAAATC AACAGAGAGC TGCAGAGGGT GAACTCTACA TTGATGATGG TCATACCTTC 
AGCTATCAAA AGAAGGAATA TATTCACCGG AGGCTTACAT TTTCCAACAA CAACCTCTCC 
TCTGTAAACT TGAATTCAGA CAGCCAGTTT ACTACTGCTT CTTGGATTGA GCGCATCATT 
ATTATTGGTG CCAGTAAGCC CAGCAAGGTT ACACTTCAGA CAGTTGATGG CCAGGAGAGC 
ACCATTGAGT TTGATTTTGA TGCCTCCATG TCTGTGCTGA CTCTCCGTAA ACCGGGCATG 
AATGCTGGGG AAGACTGGAA GGTGATGCTG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020779098.1
CDS	29..2884
Translation	MAAFTERMAPVLAFILVLCLGETWAVDRGNFKTCDQSAFCKRQRALKPGESPYRALLETMELTNTRLTLQLINDNNKVRLLLELYRLQGNMTRVKINELKPLKPRYEVPDVLIKEPPTEPLALLSQDENGVVLSLGAESQRVIVSARPFRLDIMEGHDVLMSLNSRGLLAFEHLRMRKDTFSYKVTSTVASVWDNVKRVFSSQAEPDAEKKEDVDPANQPEASKEEDEKIEEGMWEETFKSHSDTKPNGPSAISLDFSLPGVEHVYGIPEHADTLKLKTTENGDPYRLYNLDVFQYELYNPMALYGAVPVMLAHNTQRTTGIFWLNAAETWVDISSNTAGKTVFGKMLDYVQGSSEKPQTDVRWISESGIIDVFIMLGPTPKDVFSQYASLTGTQSFPPFAALGYHQCRWNYNDQEDVKNVDAGFDEHDIPYDFIWLDIEHTDGKRYFTWDPHKFASPKEMLQGIKEKKRKMVAIVDPHIKVDSNYKIHNEIRSKGFYVKNKDGGDYEGWCWPGSAGYPDFTRADMRAWWASMFAYDQYEGSMENLYTWNDMNEPSVFNGPEVTMHKDAMHQDWEHRDVHNLYGLYVQMATAEGQIQRSGGVERPFVLTRAFFAGSQRFGAVWTGDNAAEWDHLKISIPMCLSLGLVGISFCGADVGGFFKSPSSELLVRWYQTGAYQPFFRAHAHLDTPRREPWLFGPENTALIRDAVRQRYTLLPYWYQLFYHAYLTGEPVMRPLWIEYPQDPATFAIDDEFLIGKDLLVHPVTEEGSRGVTAYLPGKDDVWYDVQTFQKHNGAQNLYIPVTMSSIPVFQRGGSIIPRKLRVRRSSTCMENDPYTLYIALNQQRAAEGELYIDDGHTFSYQKKEYIHRRLTFSNNNLSSVNLNSDSQFTTASWIERIIIIGASKPSKVTLQTVDGQESTIEFDFDASMSVLTLRKPGMNAGEDWKVMLQ

Target ORF information:

RefSeq Version	XM_020923439.1
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Definition	Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020923439.1

ORF Insert Sequence:

ATGGCCGCCT TCACTGAAAG GATGGCGCCT GTCCTAGCAT TTATACTGGT TCTCTGTCTT 
GGGGAGACAT GGGCTGTGGA CAGGGGCAAC TTTAAAACCT GTGACCAGAG TGCCTTCTGC 
AAGCGCCAGC GTGCATTGAA GCCAGGGGAA TCTCCTTATC GAGCCCTGCT AGAGACGATG 
GAGCTGACTA ACACTAGACT AACACTTCAG CTCATCAATG ATAACAATAA GGTGCGTCTG 
TTGCTTGAGC TCTACCGTCT CCAGGGAAAC ATGACAAGGG TGAAGATTAA TGAGCTGAAG 
CCTCTGAAGC CGCGCTATGA GGTCCCAGAT GTCCTCATCA AGGAGCCACC CACTGAGCCT 
CTGGCACTTT TATCCCAAGA TGAGAATGGG GTGGTACTGT CCCTGGGTGC AGAGTCCCAG 
AGGGTTATTG TTAGCGCACG TCCCTTTCGT TTGGATATCA TGGAGGGGCA TGACGTGCTG 
ATGTCACTGA ACTCGCGTGG ACTCTTGGCT TTTGAGCACT TGAGGATGCG CAAGGACACT 
TTCTCCTATA AAGTAACTAG CACAGTGGCT AGTGTGTGGG ATAATGTCAA GAGGGTTTTC 
TCTAGTCAAG CAGAGCCAGA TGCAGAGAAG AAAGAAGATG TTGATCCCGC TAACCAGCCT 
GAGGCATCAA AAGAAGAGGA TGAGAAAATA GAAGAAGGCA TGTGGGAAGA AACGTTCAAG 
TCTCACTCAG ACACCAAACC AAATGGTCCT TCAGCTATAA GTTTAGACTT TTCTTTGCCT 
GGAGTGGAAC ATGTGTATGG CATCCCCGAA CATGCAGATA CTCTTAAACT TAAAACAACT 
GAAAATGGAG ATCCATATCG GCTGTATAAC TTGGATGTGT TCCAGTATGA ACTTTATAAT 
CCTATGGCTC TTTATGGAGC GGTCCCAGTT ATGTTGGCCC ACAACACACA GCGGACGACT 
GGTATTTTCT GGCTGAATGC TGCAGAGACG TGGGTGGATA TCAGCTCTAA CACAGCTGGA 
AAAACTGTTT TTGGGAAGAT GCTAGATTAT GTGCAGGGAT CAAGTGAGAA GCCACAAACT 
GATGTCCGTT GGATCTCTGA AAGTGGCATT ATTGATGTAT TCATAATGCT TGGACCAACA 
CCTAAAGACG TGTTTTCACA ATATGCCTCA CTCACAGGCA CACAGTCTTT TCCACCTTTT 
GCTGCTCTGG GCTACCACCA GTGTCGATGG AACTATAATG ATCAGGAGGA TGTAAAGAAT 
GTTGATGCTG GCTTTGACGA ACATGACATC CCCTATGACT TTATTTGGCT TGATATTGAG 
CACACTGATG GAAAGCGCTA CTTCACCTGG GATCCTCACA AGTTTGCCAG TCCTAAGGAG 
ATGCTGCAGG GTATCAAGGA GAAGAAACGC AAGATGGTGG CCATTGTTGA CCCACATATC 
AAAGTTGACA GCAATTACAA GATCCATAAC GAAATCCGGT CAAAAGGTTT TTATGTTAAG 
AACAAGGATG GTGGAGACTA TGAGGGCTGG TGCTGGCCAG GTAGTGCTGG TTATCCAGAT 
TTCACCCGTG CAGACATGAG GGCTTGGTGG GCCAGCATGT TTGCCTATGA TCAATATGAA 
GGTAGTATGG AAAACTTGTA CACATGGAAT GACATGAATG AGCCATCGGT TTTTAATGGA 
CCAGAGGTCA CCATGCACAA AGATGCCATG CACCAAGACT GGGAGCATCG TGATGTTCAT 
AACTTATATG GGCTTTATGT GCAAATGGCC ACAGCTGAAG GTCAGATCCA GAGATCTGGA 
GGTGTAGAGC GTCCATTCGT CCTTACCAGG GCCTTTTTTG CGGGATCACA GCGCTTTGGT 
GCTGTGTGGA CTGGCGACAA TGCCGCGGAG TGGGACCATC TTAAGATCTC TATCCCAATG 
TGCTTGAGCC TTGGCTTGGT TGGAATTTCT TTTTGTGGAG CTGACGTGGG AGGCTTTTTC 
AAGTCTCCGA GCTCTGAGCT TCTGGTGCGT TGGTACCAAA CCGGAGCCTA CCAGCCATTC 
TTCCGGGCAC ATGCTCACCT GGACACGCCA CGCCGGGAGC CATGGCTCTT TGGTCCTGAG 
AACACTGCCC TGATCCGTGA CGCAGTGCGC CAGCGCTACA CTCTGCTGCC CTACTGGTAC 
CAACTGTTCT ACCATGCATA CCTAACTGGA GAGCCTGTCA TGAGACCATT GTGGATAGAG 
TATCCTCAAG ATCCTGCTAC TTTTGCCATT GATGATGAGT TTCTCATTGG AAAAGACTTG 
TTGGTGCATC CTGTGACCGA GGAGGGATCT AGGGGAGTCA CTGCATACCT GCCAGGGAAA 
GATGATGTCT GGTATGATGT CCAAACGTTC CAGAAGCACA ACGGGGCCCA AAACCTTTAC 
ATTCCTGTGA CAATGAGTAG TATTCCAGTT TTCCAGCGTG GTGGTTCCAT TATTCCAAGG 
AAGCTCCGTG TTCGCAGATC ATCAACTTGT ATGGAAAATG ATCCTTACAC TTTATACATT 
GCCTTAAATC AACAGAGAGC TGCAGAGGGT GAACTCTACA TTGATGATGG TCATACCTTC 
AGCTATCAAA AGAAGGAATA TATTCACCGG AGGCTTACAT TTTCCAACAA CAACCTCTCC 
TCTGTAAACT TGAATTCAGA CAGCCAGTTT ACTACTGCTT CTTGGATTGA GCGCATCATT 
ATTATTGGTG CCAGTAAGCC CAGCAAGGTT ACACTTCAGA CAGTTGATGG CCAGGAGAGC 
ACCATTGAGT TTGATTTTGA TGCCTCCATG TCTGTGCTGA CTCTCCGTAA ACCGGGCATG 
AATGCTGGGG AAGACTGGAA GGTGATGCTG CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ganab ( XM_020923440.1 ) cDNA ORF clone, Boleophthalmus pectinirostris(great blue-spotted mudskipper) -> XP_020779099.1 Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo91116
Clone ID Related Accession (Same CDS sequence)	XM_020923440.1
Accession Version	XM_020923440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Product	neutral alpha-glucosidase AB isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018354349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Boleophthalmus pectinirostris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGCCT TCACTGAAAG GATGGCGCCT GTCCTAGCAT TTATACTGGT TCTCTGTCTT 
GGGGAGACAT GGGCTGTGGA CAGGGGCAAC TTTAAAACCT GTGACCAGAG TGCCTTCTGC 
AAGCGCCAGC GTGCATTGAA GCCAGGGGAA TCTCCTTATC GAGCCCTGCT AGAGACGATG 
GAGCTGACTA ACACTAGACT AACACTTCAG CTCATCAATG ATAACAATAA GGTGCGTCTG 
TTGCTTGAGC TCTACCGTCT CCAGGGAAAC ATGACAAGGG TGAAGATTAA TGAGCTGAAG 
CCTCTGAAGC CGCGCTATGA GGTCCCAGAT GTCCTCATCA AGGAGCCACC CACTGAGCCT 
CTGGCACTTT TATCCCAAGA TGAGAATGGG GTGGTACTGT CCCTGGGTGC AGAGTCCCAG 
AGGGTTATTG TTAGCGCACG TCCCTTTCGT TTGGATATCA TGGAGGGGCA TGACGTGCTG 
ATGTCACTGA ACTCGCGTGG ACTCTTGGCT TTTGAGCACT TGAGGATGCG CAAGGACACT 
CAAGCAGAGC CAGATGCAGA GAAGAAAGAA GATGTTGATC CCGCTAACCA GCCTGAGGCA 
TCAAAAGAAG AGGATGAGAA AATAGAAGAA GGCATGTGGG AAGAAACGTT CAAGTCTCAC 
TCAGACACCA AACCAAATGG TCCTTCAGCT ATAAGTTTAG ACTTTTCTTT GCCTGGAGTG 
GAACATGTGT ATGGCATCCC CGAACATGCA GATACTCTTA AACTTAAAAC AACTGAAAAT 
GGAGATCCAT ATCGGCTGTA TAACTTGGAT GTGTTCCAGT ATGAACTTTA TAATCCTATG 
GCTCTTTATG GAGCGGTCCC AGTTATGTTG GCCCACAACA CACAGCGGAC GACTGGTATT 
TTCTGGCTGA ATGCTGCAGA GACGTGGGTG GATATCAGCT CTAACACAGC TGGAAAAACT 
GTTTTTGGGA AGATGCTAGA TTATGTGCAG GGATCAAGTG AGAAGCCACA AACTGATGTC 
CGTTGGATCT CTGAAAGTGG CATTATTGAT GTATTCATAA TGCTTGGACC AACACCTAAA 
GACGTGTTTT CACAATATGC CTCACTCACA GGCACACAGT CTTTTCCACC TTTTGCTGCT 
CTGGGCTACC ACCAGTGTCG ATGGAACTAT AATGATCAGG AGGATGTAAA GAATGTTGAT 
GCTGGCTTTG ACGAACATGA CATCCCCTAT GACTTTATTT GGCTTGATAT TGAGCACACT 
GATGGAAAGC GCTACTTCAC CTGGGATCCT CACAAGTTTG CCAGTCCTAA GGAGATGCTG 
CAGGGTATCA AGGAGAAGAA ACGCAAGATG GTGGCCATTG TTGACCCACA TATCAAAGTT 
GACAGCAATT ACAAGATCCA TAACGAAATC CGGTCAAAAG GTTTTTATGT TAAGAACAAG 
GATGGTGGAG ACTATGAGGG CTGGTGCTGG CCAGGTAGTG CTGGTTATCC AGATTTCACC 
CGTGCAGACA TGAGGGCTTG GTGGGCCAGC ATGTTTGCCT ATGATCAATA TGAAGGTAGT 
ATGGAAAACT TGTACACATG GAATGACATG AATGAGCCAT CGGTTTTTAA TGGACCAGAG 
GTCACCATGC ACAAAGATGC CATGCACCAA GACTGGGAGC ATCGTGATGT TCATAACTTA 
TATGGGCTTT ATGTGCAAAT GGCCACAGCT GAAGGTCAGA TCCAGAGATC TGGAGGTGTA 
GAGCGTCCAT TCGTCCTTAC CAGGGCCTTT TTTGCGGGAT CACAGCGCTT TGGTGCTGTG 
TGGACTGGCG ACAATGCCGC GGAGTGGGAC CATCTTAAGA TCTCTATCCC AATGTGCTTG 
AGCCTTGGCT TGGTTGGAAT TTCTTTTTGT GGAGCTGACG TGGGAGGCTT TTTCAAGTCT 
CCGAGCTCTG AGCTTCTGGT GCGTTGGTAC CAAACCGGAG CCTACCAGCC ATTCTTCCGG 
GCACATGCTC ACCTGGACAC GCCACGCCGG GAGCCATGGC TCTTTGGTCC TGAGAACACT 
GCCCTGATCC GTGACGCAGT GCGCCAGCGC TACACTCTGC TGCCCTACTG GTACCAACTG 
TTCTACCATG CATACCTAAC TGGAGAGCCT GTCATGAGAC CATTGTGGAT AGAGTATCCT 
CAAGATCCTG CTACTTTTGC CATTGATGAT GAGTTTCTCA TTGGAAAAGA CTTGTTGGTG 
CATCCTGTGA CCGAGGAGGG ATCTAGGGGA GTCACTGCAT ACCTGCCAGG GAAAGATGAT 
GTCTGGTATG ATGTCCAAAC GTTCCAGAAG CACAACGGGG CCCAAAACCT TTACATTCCT 
GTGACAATGA GTAGTATTCC AGTTTTCCAG CGTGGTGGTT CCATTATTCC AAGGAAGCTC 
CGTGTTCGCA GATCATCAAC TTGTATGGAA AATGATCCTT ACACTTTATA CATTGCCTTA 
AATCAACAGA GAGCTGCAGA GGGTGAACTC TACATTGATG ATGGTCATAC CTTCAGCTAT 
CAAAAGAAGG AATATATTCA CCGGAGGCTT ACATTTTCCA ACAACAACCT CTCCTCTGTA 
AACTTGAATT CAGACAGCCA GTTTACTACT GCTTCTTGGA TTGAGCGCAT CATTATTATT 
GGTGCCAGTA AGCCCAGCAA GGTTACACTT CAGACAGTTG ATGGCCAGGA GAGCACCATT 
GAGTTTGATT TTGATGCCTC CATGTCTGTG CTGACTCTCC GTAAACCGGG CATGAATGCT 
GGGGAAGACT GGAAGGTGAT GCTGCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020779099.1
CDS	29..2818
Translation	MAAFTERMAPVLAFILVLCLGETWAVDRGNFKTCDQSAFCKRQRALKPGESPYRALLETMELTNTRLTLQLINDNNKVRLLLELYRLQGNMTRVKINELKPLKPRYEVPDVLIKEPPTEPLALLSQDENGVVLSLGAESQRVIVSARPFRLDIMEGHDVLMSLNSRGLLAFEHLRMRKDTQAEPDAEKKEDVDPANQPEASKEEDEKIEEGMWEETFKSHSDTKPNGPSAISLDFSLPGVEHVYGIPEHADTLKLKTTENGDPYRLYNLDVFQYELYNPMALYGAVPVMLAHNTQRTTGIFWLNAAETWVDISSNTAGKTVFGKMLDYVQGSSEKPQTDVRWISESGIIDVFIMLGPTPKDVFSQYASLTGTQSFPPFAALGYHQCRWNYNDQEDVKNVDAGFDEHDIPYDFIWLDIEHTDGKRYFTWDPHKFASPKEMLQGIKEKKRKMVAIVDPHIKVDSNYKIHNEIRSKGFYVKNKDGGDYEGWCWPGSAGYPDFTRADMRAWWASMFAYDQYEGSMENLYTWNDMNEPSVFNGPEVTMHKDAMHQDWEHRDVHNLYGLYVQMATAEGQIQRSGGVERPFVLTRAFFAGSQRFGAVWTGDNAAEWDHLKISIPMCLSLGLVGISFCGADVGGFFKSPSSELLVRWYQTGAYQPFFRAHAHLDTPRREPWLFGPENTALIRDAVRQRYTLLPYWYQLFYHAYLTGEPVMRPLWIEYPQDPATFAIDDEFLIGKDLLVHPVTEEGSRGVTAYLPGKDDVWYDVQTFQKHNGAQNLYIPVTMSSIPVFQRGGSIIPRKLRVRRSSTCMENDPYTLYIALNQQRAAEGELYIDDGHTFSYQKKEYIHRRLTFSNNNLSSVNLNSDSQFTTASWIERIIIIGASKPSKVTLQTVDGQESTIEFDFDASMSVLTLRKPGMNAGEDWKVMLQ

Target ORF information:

RefSeq Version	XM_020923440.1
Organism	Boleophthalmus pectinirostris(great blue-spotted mudskipper)
Definition	Boleophthalmus pectinirostris glucosidase II alpha subunit (ganab), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020923440.1

ORF Insert Sequence:

ATGGCCGCCT TCACTGAAAG GATGGCGCCT GTCCTAGCAT TTATACTGGT TCTCTGTCTT 
GGGGAGACAT GGGCTGTGGA CAGGGGCAAC TTTAAAACCT GTGACCAGAG TGCCTTCTGC 
AAGCGCCAGC GTGCATTGAA GCCAGGGGAA TCTCCTTATC GAGCCCTGCT AGAGACGATG 
GAGCTGACTA ACACTAGACT AACACTTCAG CTCATCAATG ATAACAATAA GGTGCGTCTG 
TTGCTTGAGC TCTACCGTCT CCAGGGAAAC ATGACAAGGG TGAAGATTAA TGAGCTGAAG 
CCTCTGAAGC CGCGCTATGA GGTCCCAGAT GTCCTCATCA AGGAGCCACC CACTGAGCCT 
CTGGCACTTT TATCCCAAGA TGAGAATGGG GTGGTACTGT CCCTGGGTGC AGAGTCCCAG 
AGGGTTATTG TTAGCGCACG TCCCTTTCGT TTGGATATCA TGGAGGGGCA TGACGTGCTG 
ATGTCACTGA ACTCGCGTGG ACTCTTGGCT TTTGAGCACT TGAGGATGCG CAAGGACACT 
CAAGCAGAGC CAGATGCAGA GAAGAAAGAA GATGTTGATC CCGCTAACCA GCCTGAGGCA 
TCAAAAGAAG AGGATGAGAA AATAGAAGAA GGCATGTGGG AAGAAACGTT CAAGTCTCAC 
TCAGACACCA AACCAAATGG TCCTTCAGCT ATAAGTTTAG ACTTTTCTTT GCCTGGAGTG 
GAACATGTGT ATGGCATCCC CGAACATGCA GATACTCTTA AACTTAAAAC AACTGAAAAT 
GGAGATCCAT ATCGGCTGTA TAACTTGGAT GTGTTCCAGT ATGAACTTTA TAATCCTATG 
GCTCTTTATG GAGCGGTCCC AGTTATGTTG GCCCACAACA CACAGCGGAC GACTGGTATT 
TTCTGGCTGA ATGCTGCAGA GACGTGGGTG GATATCAGCT CTAACACAGC TGGAAAAACT 
GTTTTTGGGA AGATGCTAGA TTATGTGCAG GGATCAAGTG AGAAGCCACA AACTGATGTC 
CGTTGGATCT CTGAAAGTGG CATTATTGAT GTATTCATAA TGCTTGGACC AACACCTAAA 
GACGTGTTTT CACAATATGC CTCACTCACA GGCACACAGT CTTTTCCACC TTTTGCTGCT 
CTGGGCTACC ACCAGTGTCG ATGGAACTAT AATGATCAGG AGGATGTAAA GAATGTTGAT 
GCTGGCTTTG ACGAACATGA CATCCCCTAT GACTTTATTT GGCTTGATAT TGAGCACACT 
GATGGAAAGC GCTACTTCAC CTGGGATCCT CACAAGTTTG CCAGTCCTAA GGAGATGCTG 
CAGGGTATCA AGGAGAAGAA ACGCAAGATG GTGGCCATTG TTGACCCACA TATCAAAGTT 
GACAGCAATT ACAAGATCCA TAACGAAATC CGGTCAAAAG GTTTTTATGT TAAGAACAAG 
GATGGTGGAG ACTATGAGGG CTGGTGCTGG CCAGGTAGTG CTGGTTATCC AGATTTCACC 
CGTGCAGACA TGAGGGCTTG GTGGGCCAGC ATGTTTGCCT ATGATCAATA TGAAGGTAGT 
ATGGAAAACT TGTACACATG GAATGACATG AATGAGCCAT CGGTTTTTAA TGGACCAGAG 
GTCACCATGC ACAAAGATGC CATGCACCAA GACTGGGAGC ATCGTGATGT TCATAACTTA 
TATGGGCTTT ATGTGCAAAT GGCCACAGCT GAAGGTCAGA TCCAGAGATC TGGAGGTGTA 
GAGCGTCCAT TCGTCCTTAC CAGGGCCTTT TTTGCGGGAT CACAGCGCTT TGGTGCTGTG 
TGGACTGGCG ACAATGCCGC GGAGTGGGAC CATCTTAAGA TCTCTATCCC AATGTGCTTG 
AGCCTTGGCT TGGTTGGAAT TTCTTTTTGT GGAGCTGACG TGGGAGGCTT TTTCAAGTCT 
CCGAGCTCTG AGCTTCTGGT GCGTTGGTAC CAAACCGGAG CCTACCAGCC ATTCTTCCGG 
GCACATGCTC ACCTGGACAC GCCACGCCGG GAGCCATGGC TCTTTGGTCC TGAGAACACT 
GCCCTGATCC GTGACGCAGT GCGCCAGCGC TACACTCTGC TGCCCTACTG GTACCAACTG 
TTCTACCATG CATACCTAAC TGGAGAGCCT GTCATGAGAC CATTGTGGAT AGAGTATCCT 
CAAGATCCTG CTACTTTTGC CATTGATGAT GAGTTTCTCA TTGGAAAAGA CTTGTTGGTG 
CATCCTGTGA CCGAGGAGGG ATCTAGGGGA GTCACTGCAT ACCTGCCAGG GAAAGATGAT 
GTCTGGTATG ATGTCCAAAC GTTCCAGAAG CACAACGGGG CCCAAAACCT TTACATTCCT 
GTGACAATGA GTAGTATTCC AGTTTTCCAG CGTGGTGGTT CCATTATTCC AAGGAAGCTC 
CGTGTTCGCA GATCATCAAC TTGTATGGAA AATGATCCTT ACACTTTATA CATTGCCTTA 
AATCAACAGA GAGCTGCAGA GGGTGAACTC TACATTGATG ATGGTCATAC CTTCAGCTAT 
CAAAAGAAGG AATATATTCA CCGGAGGCTT ACATTTTCCA ACAACAACCT CTCCTCTGTA 
AACTTGAATT CAGACAGCCA GTTTACTACT GCTTCTTGGA TTGAGCGCAT CATTATTATT 
GGTGCCAGTA AGCCCAGCAA GGTTACACTT CAGACAGTTG ATGGCCAGGA GAGCACCATT 
GAGTTTGATT TTGATGCCTC CATGTCTGTG CTGACTCTCC GTAAACCGGG CATGAATGCT 
GGGGAAGACT GGAAGGTGAT GCTGCAGTAA