DLAT cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	DLAT
Entrez Gene ID	105005035
Full Name	dihydrolipoamide S-acetyltransferase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010862882.1	XP_010861184.1	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DLAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DLAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi81651	XM_010862882.1 Latest version!	Bison bison bison dihydrolipoamide S-acetyltransferase (DLAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DLAT ( XM_010862882.1 ) cDNA ORF clone, Bison bison bison -> XP_010861184.1 Bison bison bison dihydrolipoamide S-acetyltransferase (DLAT), mRNA.

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CloneID	OBi81651
Clone ID Related Accession (Same CDS sequence)	XM_010862882.1
Accession Version	XM_010862882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011495045.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGCAGCCC CCCGGGCGGG ATTCGGGGCT 
CGGTGGACGG CCTTTCGGGA GGAGCCGGGA GCTCCGTGCG TGACCCCGCA GGCTGGCTCG 
GCTCTGGCTC GCTGCAGCAG CAAGACCCCA GGGTATGGCA GGGTCCGGGC ACTCTGCGGC 
TGGAGCCCAG TCTCTCGGGC CACACCGCGG AATCGCGTCT TGCTGCAGCT CTGGGGATCG 
CCTAGCCGCC GCTGGTATAG CCTACCCCCG CATCAGAAGG TTCCATTGCC TTCACTGTCC 
CCTACAATGC AGGCAGGCAC CATAGCCCGC TGGGAAAAGA AGGAAGGAGA AAAAATCAAT 
GAGGGTGAAC TAATTGCAGA GGTTGAAACC GATAAAGCTA CTGTTGGATT TGAGAGTGTG 
GAGGAGTGTT ACATGGCAAA GATCCTTGTT GCTGAAGGTA CCAGAGATGT TCCAGTTGGA 
GCAATCATCT GTATCACAGT TGACAAGCCT GAAGATGTTG AAGCCTTTAA AAATTACACA 
CTGGATTCTT CAGCAGCACC TGCCCCACCA GCAGCCCCGG CACCAACCCC TGCCGCCCCC 
GCTCCATCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCTAC TCACATGCAG 
GTGCTTCTTC CTGCCCTGTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA 
AAAGTGGGTG AGAAGCTAAA TGAAGGAGAT TTATTGGCAG AGATAGAGAC TGACAAGGCC 
ACTATAGGTT TTGAGGTACA GGAAGAAGGT TACCTGGCAA AAATCCTGAT CCCTGAAGGC 
ACACGAGATG TCCCTCTAGG AACCCCGCTC TGTATCATTG TGGAAAAAGA GGCAGATATA 
CCAGCATTTG CTGACTATCG GCCAGCTGAA GTAACTGATT TAAAACCACC AGCACCACCA 
CCTATCCCAT CTCCGGCAGC CCCTGTTCCT CCAGCTCCCC AACCTGTAGC CCCTCCACCT 
TCAGCCCCCC GGCCAGCCGC TCCAGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCGCTT 
GCGAAGAAGT TGGCAGCAGA GAAAGGGATT GACCTTACAC AAGTAAAAGG GACAGGACCA 
GATGGCAGAA TCATCAAGAA GGACATTGAC TCTTTTGTGC CTACTAAAGC TGCTCCTACT 
CCAGCAGCCG CCGTGCCTCC CCCAAGTCCA GGAGTGGCTC CAGTTCCTAC AGGTGTCTTC 
ACAGATATCC CAATCAGCAA CATTCGCCGA GTCATTGCAC AGCGGTTAAT GCAATCTAAG 
CAAACCATAC CTCATTATTA CCTTTCTATT GATGTAAATA TGGGAGAAGT TTTGTTGGTA 
CGGAAAGAAC TTAATAAGAT GTTAGAAGGA AAAAGTAAAA TTTCCGTCAA TGACTTCATT 
ATAAAAGCTT CAGCTTTGGC ATGTTTAAAG GTTCCTGAAG CAAATTCTTC TTGGATGGAC 
ACAGTTATAA GACAAAATCA TGTTGTTGAT ATCAGTGTTG CAGTCAGTAC TCCTGCAGGA 
CTCATCACAC CTATTGTATT TAATGCACAT ATAAAAGGAC TGGAAACCAT TGCTAATGAC 
GTTGTTTCTC TAGCAACCAA AGCACGAGAG GGTAAACTAC AACCACATGA GTTCCAGGGT 
GGCACTTTTA CGATCTCAAA TTTAGGAATG TTTGGGATTA AGAACTTCTC TGCTATTATA 
AACCCACCTC AAGCATGTAT TTTGGCAATT GGTGCTTCAG AGGATAGACT GGTTCCAGCA 
GATAATGAAA AAGGATTTGA TGTGGCCAGC ATGATGTCTG TTACACTCAG TTGTGATCAT 
CGGGTTGTCG ACGGAGCAGT TGGAGCCCAG TGGCTTGCTG AGTTTAGAAA GTACCTTGAA 
AAACCTATCA CCATGTTGTT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010861184.1
CDS	86..2029
Translation	MWRVCARRAQNAAPRAGFGARWTAFREEPGAPCVTPQAGSALARCSSKTPGYGRVRALCGWSPVSRATPRNRVLLQLWGSPSRRWYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGEKINEGELIAEVETDKATVGFESVEECYMAKILVAEGTRDVPVGAIICITVDKPEDVEAFKNYTLDSSAAPAPPAAPAPTPAAPAPSPTPSAQAPGSSYPTHMQVLLPALSPTMTMGTVQRWEKKVGEKLNEGDLLAEIETDKATIGFEVQEEGYLAKILIPEGTRDVPLGTPLCIIVEKEADIPAFADYRPAEVTDLKPPAPPPIPSPAAPVPPAPQPVAPPPSAPRPAAPAGPKGRVFVSPLAKKLAAEKGIDLTQVKGTGPDGRIIKKDIDSFVPTKAAPTPAAAVPPPSPGVAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKMLEGKSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDISVAVSTPAGLITPIVFNAHIKGLETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDRLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL

Target ORF information:

RefSeq Version	XM_010862882.1
Organism	Bison bison bison
Definition	Bison bison bison dihydrolipoamide S-acetyltransferase (DLAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010862882.1

ORF Insert Sequence:

ATGTGGCGCG TCTGTGCGCG ACGGGCTCAG AATGCAGCCC CCCGGGCGGG ATTCGGGGCT 
CGGTGGACGG CCTTTCGGGA GGAGCCGGGA GCTCCGTGCG TGACCCCGCA GGCTGGCTCG 
GCTCTGGCTC GCTGCAGCAG CAAGACCCCA GGGTATGGCA GGGTCCGGGC ACTCTGCGGC 
TGGAGCCCAG TCTCTCGGGC CACACCGCGG AATCGCGTCT TGCTGCAGCT CTGGGGATCG 
CCTAGCCGCC GCTGGTATAG CCTACCCCCG CATCAGAAGG TTCCATTGCC TTCACTGTCC 
CCTACAATGC AGGCAGGCAC CATAGCCCGC TGGGAAAAGA AGGAAGGAGA AAAAATCAAT 
GAGGGTGAAC TAATTGCAGA GGTTGAAACC GATAAAGCTA CTGTTGGATT TGAGAGTGTG 
GAGGAGTGTT ACATGGCAAA GATCCTTGTT GCTGAAGGTA CCAGAGATGT TCCAGTTGGA 
GCAATCATCT GTATCACAGT TGACAAGCCT GAAGATGTTG AAGCCTTTAA AAATTACACA 
CTGGATTCTT CAGCAGCACC TGCCCCACCA GCAGCCCCGG CACCAACCCC TGCCGCCCCC 
GCTCCATCAC CTACACCTTC TGCTCAGGCT CCTGGTAGCT CATATCCTAC TCACATGCAG 
GTGCTTCTTC CTGCCCTGTC TCCCACCATG ACCATGGGCA CAGTTCAGAG ATGGGAAAAA 
AAAGTGGGTG AGAAGCTAAA TGAAGGAGAT TTATTGGCAG AGATAGAGAC TGACAAGGCC 
ACTATAGGTT TTGAGGTACA GGAAGAAGGT TACCTGGCAA AAATCCTGAT CCCTGAAGGC 
ACACGAGATG TCCCTCTAGG AACCCCGCTC TGTATCATTG TGGAAAAAGA GGCAGATATA 
CCAGCATTTG CTGACTATCG GCCAGCTGAA GTAACTGATT TAAAACCACC AGCACCACCA 
CCTATCCCAT CTCCGGCAGC CCCTGTTCCT CCAGCTCCCC AACCTGTAGC CCCTCCACCT 
TCAGCCCCCC GGCCAGCCGC TCCAGCTGGA CCAAAGGGAA GGGTGTTTGT TAGCCCGCTT 
GCGAAGAAGT TGGCAGCAGA GAAAGGGATT GACCTTACAC AAGTAAAAGG GACAGGACCA 
GATGGCAGAA TCATCAAGAA GGACATTGAC TCTTTTGTGC CTACTAAAGC TGCTCCTACT 
CCAGCAGCCG CCGTGCCTCC CCCAAGTCCA GGAGTGGCTC CAGTTCCTAC AGGTGTCTTC 
ACAGATATCC CAATCAGCAA CATTCGCCGA GTCATTGCAC AGCGGTTAAT GCAATCTAAG 
CAAACCATAC CTCATTATTA CCTTTCTATT GATGTAAATA TGGGAGAAGT TTTGTTGGTA 
CGGAAAGAAC TTAATAAGAT GTTAGAAGGA AAAAGTAAAA TTTCCGTCAA TGACTTCATT 
ATAAAAGCTT CAGCTTTGGC ATGTTTAAAG GTTCCTGAAG CAAATTCTTC TTGGATGGAC 
ACAGTTATAA GACAAAATCA TGTTGTTGAT ATCAGTGTTG CAGTCAGTAC TCCTGCAGGA 
CTCATCACAC CTATTGTATT TAATGCACAT ATAAAAGGAC TGGAAACCAT TGCTAATGAC 
GTTGTTTCTC TAGCAACCAA AGCACGAGAG GGTAAACTAC AACCACATGA GTTCCAGGGT 
GGCACTTTTA CGATCTCAAA TTTAGGAATG TTTGGGATTA AGAACTTCTC TGCTATTATA 
AACCCACCTC AAGCATGTAT TTTGGCAATT GGTGCTTCAG AGGATAGACT GGTTCCAGCA 
GATAATGAAA AAGGATTTGA TGTGGCCAGC ATGATGTCTG TTACACTCAG TTGTGATCAT 
CGGGTTGTCG ACGGAGCAGT TGGAGCCCAG TGGCTTGCTG AGTTTAGAAA GTACCTTGAA 
AAACCTATCA CCATGTTGTT ATAA