EHHADH cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	EHHADH
Entrez Gene ID	104998607
Full Name	enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010853866.1	XP_010852168.1	peroxisomal bifunctional enzyme

Pathways from BioSystems
Gene Ontology

The following EHHADH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EHHADH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi75023	XM_010853866.1 Latest version!	Bison bison bison enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EHHADH ( XM_010853866.1 ) cDNA ORF clone, Bison bison bison -> XP_010852168.1 Bison bison bison enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH), mRNA.

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CloneID	OBi75023
Clone ID Related Accession (Same CDS sequence)	XM_010853866.1
Accession Version	XM_010853866.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	peroxisomal bifunctional enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494905.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGT TCACGCGGCT GCAGAACTGC CTGGCACTGA TCCGTCTACG AAACCCGCCA 
GTCAATGCGA TCAGTACAAC TGTTGCCCGT GGAATAAAAG AGAGTCTGCA GAAAGCTATA 
ACAGATGATA CAGTTAAAGC CATTGTGATT TGTGGAGCAG ATGGCATCTT CTGTGCAGGT 
GCCGATATTA GAGAATTCAA AGTTCATAAG ACCTTTGACA TACAACTGGG AGATATAGTA 
GATGAAATAC AGAGAAACAA GAAGCCTGTG GTGGCAGCTA TCCAAAAGTT GGCTTTAGGA 
GGGGGACTGG AGCTGGCCCT GGGCTGTCAC TATAGAATTG CCCATGCAGA GGCTCAAGTT 
GGCTTCCCAG AAGTCACACT AGGAATCCTT CCTGGTGCAA GAGGAACCCA GCTTCTCCCC 
AGACTTGTTG GAGTGCCTGC TGCACTTGAC TTGATTATCT CAGGAAGACA TATTTCAGCA 
AATGAAGCAC TTAAGCTGGG TATTATAGAT AAAATTGTGA ACTCAGACCC AATTGAAGAA 
TCGATCAAAT TTGCCCAGAG AATTTCAGAT CAATCTCCAG AATCCCGTAG AATCTGCAAC 
ATGCCAGTCC CGAGCTTGCC CAACATGGAT GCCATTTTCA GCGAAGCCCT TCTGCAGGTG 
CGGAAGCGGT ACCCTGGGTG GCTGTCTCCG GAGGCCTGTG TCCAAGCAGT CCAGGCTGCT 
GTCCAATATC CATATGACAT GGGCAGCAAG AAGGAAAAGG AGTTGGGCCT GTACCTTAAT 
ACCTCAGGGC AGGCCAAGGC CCTGCAATAT GTTTTCTTTG CTGAGAGGAT GGCAAATAAG 
TGGTCCACTC CCTCTGGAGC TTCCTGGAAA ACAGCGTTGG GACGGCCCAT CTCCTCTGTT 
GGCGTTCTCG GCTTAGGAAC AATGGGCCGA GGCATTGTCA TTTCTTTTGC AAAGGCCCAG 
ATCCCTGTGA TTGCCGTAGA ATCAGACAAG AAGCAGCTAG AGACTGCAAG CAAGATAATA 
ACCTCCATCT TGGAAAAAGA AGCATCCAAA ATGCAGCAGA GTGGGCGCCC TTGGTCAGGA 
CCAAAACCCA GGTTAACTAC ATCTATGAAA GAGCTCAGTG GTGTAGATTT AGTCGTTGAA 
GCAGTTTATG AGGACATGAA CCTGAAGAAG CATGTCTTTG CTGAACTATC AGCTGTGTGC 
AAGCCAGAAG CACTTTTATG CACCAACACT TCAGCCCTGG ACATTGATGA GATTGCTTCT 
TCCAGTGACC GTCCTCACTT GGTCATTGGC ACCCACTTCT TCTCACCAGC TCATGTCATG 
AAGTTGTTAG AGGTTATTCC CAGCCGATTC TCTTCCCCCA CCACCATTGC CACGGTTATG 
AATTTATCAA AAAAGATTAA GAAGATTGGA GTAGTTGTAG GCAATTGTTT TGGATTTGTT 
GGGAATCGAA TGCTGAGGTC TTATTATGAT CAGACATACT TCTTATTAGA AGAAGGCAGT 
AAACCAGAGG AGATAGATGA GGTGCTGGAA GAGTTTGGTT TCAAAATGGG ACCTTTTAGA 
GTGTCTGATC TTGCTGGGTT GGATATAGGT TGGAAATCCC GCAAAGGGCA AGGTCTTACT 
GGACCTACAT TGCCTCCAGG AAGTTCTACC CGGAAGCGAG GCGGCCGGAG ATATTGCCCA 
ATTCCTGATA TGCTGTGTGA GTCAGGACGG TTTGGTCAGA AGACAGGTAA GGGTTGGTAC 
CTTTATGATA AACCCCTGGG GAGGATTCAC AAACCTGATC CCTGGCTTTC CCAATTTCTG 
ACACAGTACA GGGAAACGTA TCACATCAAA CCACGTATCA TTAGCCGGGA TGAGATCCTC 
GAGCGCTGCC TATATTCACT CATCAATGAG GCATTTCGCA TCTTGGGAGA AGGGATGGCG 
GCTATCCCAG AGCACATTGA TGTTATCTAT TTACACGGGT ATGGATGGCC AAGGCACAGG 
GGTGGGCCCA TGTTCTATGC TTCCACAGTT GGGTTGCCCA CAGTGCTAGA GAAGTTGCAG 
AAATACCACA GGCAGAATCC TGATATTCCC CAACTAGAGC CTTGTGACTA TCTTAAAAAA 
CTGGCTTCCC AGGGAAACCT TCCTCTGGAA GAGTGGCAAA GCTTGGCAGG ACCCCCCAGC 
AGTAAACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010852168.1
CDS	72..2243
Translation	MAEFTRLQNCLALIRLRNPPVNAISTTVARGIKESLQKAITDDTVKAIVICGADGIFCAGADIREFKVHKTFDIQLGDIVDEIQRNKKPVVAAIQKLALGGGLELALGCHYRIAHAEAQVGFPEVTLGILPGARGTQLLPRLVGVPAALDLIISGRHISANEALKLGIIDKIVNSDPIEESIKFAQRISDQSPESRRICNMPVPSLPNMDAIFSEALLQVRKRYPGWLSPEACVQAVQAAVQYPYDMGSKKEKELGLYLNTSGQAKALQYVFFAERMANKWSTPSGASWKTALGRPISSVGVLGLGTMGRGIVISFAKAQIPVIAVESDKKQLETASKIITSILEKEASKMQQSGRPWSGPKPRLTTSMKELSGVDLVVEAVYEDMNLKKHVFAELSAVCKPEALLCTNTSALDIDEIASSSDRPHLVIGTHFFSPAHVMKLLEVIPSRFSSPTTIATVMNLSKKIKKIGVVVGNCFGFVGNRMLRSYYDQTYFLLEEGSKPEEIDEVLEEFGFKMGPFRVSDLAGLDIGWKSRKGQGLTGPTLPPGSSTRKRGGRRYCPIPDMLCESGRFGQKTGKGWYLYDKPLGRIHKPDPWLSQFLTQYRETYHIKPRIISRDEILERCLYSLINEAFRILGEGMAAIPEHIDVIYLHGYGWPRHRGGPMFYASTVGLPTVLEKLQKYHRQNPDIPQLEPCDYLKKLASQGNLPLEEWQSLAGPPSSKL

Target ORF information:

RefSeq Version	XM_010853866.1
Organism	Bison bison bison
Definition	Bison bison bison enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase (EHHADH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010853866.1

ORF Insert Sequence:

ATGGCTGAGT TCACGCGGCT GCAGAACTGC CTGGCACTGA TCCGTCTACG AAACCCGCCA 
GTCAATGCGA TCAGTACAAC TGTTGCCCGT GGAATAAAAG AGAGTCTGCA GAAAGCTATA 
ACAGATGATA CAGTTAAAGC CATTGTGATT TGTGGAGCAG ATGGCATCTT CTGTGCAGGT 
GCCGATATTA GAGAATTCAA AGTTCATAAG ACCTTTGACA TACAACTGGG AGATATAGTA 
GATGAAATAC AGAGAAACAA GAAGCCTGTG GTGGCAGCTA TCCAAAAGTT GGCTTTAGGA 
GGGGGACTGG AGCTGGCCCT GGGCTGTCAC TATAGAATTG CCCATGCAGA GGCTCAAGTT 
GGCTTCCCAG AAGTCACACT AGGAATCCTT CCTGGTGCAA GAGGAACCCA GCTTCTCCCC 
AGACTTGTTG GAGTGCCTGC TGCACTTGAC TTGATTATCT CAGGAAGACA TATTTCAGCA 
AATGAAGCAC TTAAGCTGGG TATTATAGAT AAAATTGTGA ACTCAGACCC AATTGAAGAA 
TCGATCAAAT TTGCCCAGAG AATTTCAGAT CAATCTCCAG AATCCCGTAG AATCTGCAAC 
ATGCCAGTCC CGAGCTTGCC CAACATGGAT GCCATTTTCA GCGAAGCCCT TCTGCAGGTG 
CGGAAGCGGT ACCCTGGGTG GCTGTCTCCG GAGGCCTGTG TCCAAGCAGT CCAGGCTGCT 
GTCCAATATC CATATGACAT GGGCAGCAAG AAGGAAAAGG AGTTGGGCCT GTACCTTAAT 
ACCTCAGGGC AGGCCAAGGC CCTGCAATAT GTTTTCTTTG CTGAGAGGAT GGCAAATAAG 
TGGTCCACTC CCTCTGGAGC TTCCTGGAAA ACAGCGTTGG GACGGCCCAT CTCCTCTGTT 
GGCGTTCTCG GCTTAGGAAC AATGGGCCGA GGCATTGTCA TTTCTTTTGC AAAGGCCCAG 
ATCCCTGTGA TTGCCGTAGA ATCAGACAAG AAGCAGCTAG AGACTGCAAG CAAGATAATA 
ACCTCCATCT TGGAAAAAGA AGCATCCAAA ATGCAGCAGA GTGGGCGCCC TTGGTCAGGA 
CCAAAACCCA GGTTAACTAC ATCTATGAAA GAGCTCAGTG GTGTAGATTT AGTCGTTGAA 
GCAGTTTATG AGGACATGAA CCTGAAGAAG CATGTCTTTG CTGAACTATC AGCTGTGTGC 
AAGCCAGAAG CACTTTTATG CACCAACACT TCAGCCCTGG ACATTGATGA GATTGCTTCT 
TCCAGTGACC GTCCTCACTT GGTCATTGGC ACCCACTTCT TCTCACCAGC TCATGTCATG 
AAGTTGTTAG AGGTTATTCC CAGCCGATTC TCTTCCCCCA CCACCATTGC CACGGTTATG 
AATTTATCAA AAAAGATTAA GAAGATTGGA GTAGTTGTAG GCAATTGTTT TGGATTTGTT 
GGGAATCGAA TGCTGAGGTC TTATTATGAT CAGACATACT TCTTATTAGA AGAAGGCAGT 
AAACCAGAGG AGATAGATGA GGTGCTGGAA GAGTTTGGTT TCAAAATGGG ACCTTTTAGA 
GTGTCTGATC TTGCTGGGTT GGATATAGGT TGGAAATCCC GCAAAGGGCA AGGTCTTACT 
GGACCTACAT TGCCTCCAGG AAGTTCTACC CGGAAGCGAG GCGGCCGGAG ATATTGCCCA 
ATTCCTGATA TGCTGTGTGA GTCAGGACGG TTTGGTCAGA AGACAGGTAA GGGTTGGTAC 
CTTTATGATA AACCCCTGGG GAGGATTCAC AAACCTGATC CCTGGCTTTC CCAATTTCTG 
ACACAGTACA GGGAAACGTA TCACATCAAA CCACGTATCA TTAGCCGGGA TGAGATCCTC 
GAGCGCTGCC TATATTCACT CATCAATGAG GCATTTCGCA TCTTGGGAGA AGGGATGGCG 
GCTATCCCAG AGCACATTGA TGTTATCTAT TTACACGGGT ATGGATGGCC AAGGCACAGG 
GGTGGGCCCA TGTTCTATGC TTCCACAGTT GGGTTGCCCA CAGTGCTAGA GAAGTTGCAG 
AAATACCACA GGCAGAATCC TGATATTCCC CAACTAGAGC CTTGTGACTA TCTTAAAAAA 
CTGGCTTCCC AGGGAAACCT TCCTCTGGAA GAGTGGCAAA GCTTGGCAGG ACCCCCCAGC 
AGTAAACTGT GA