TWF2 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	TWF2
Entrez Gene ID	104997144
Full Name	twinfilin actin binding protein 2
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010851748.1	XP_010850050.1	twinfilin-2 isoform X1
XM_010851749.1	XP_010850051.1	twinfilin-2 isoform X2

Pathways from BioSystems
Gene Ontology

The following TWF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi73531	XM_010851748.1 Latest version!	Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBi73532	XM_010851749.1 Latest version!	Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TWF2 ( XM_010851748.1 ) cDNA ORF clone, Bison bison bison -> XP_010850050.1 Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi73531
Clone ID Related Accession (Same CDS sequence)	XM_010851748.1
Accession Version	XM_010851748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	twinfilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494876.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCATC AGACCGGCAT CCACGCCACT GAAGAGCTGA AGGAGTTCTT TGCCAAGGCG 
CGGGCTGGCT CTGTGCGACT CATCAAAGTT GTCATTGAGG ACGAACAGCT CGTGCTGGGC 
GCCTTTCGGG AGCTGGTGGG CTGCTGGGAC CAGGACTATG ACGGGGCCGT TCTGCCGCTG 
CTGGATGCCC AACAGCCTTG CTACCTGCTC TACCGCCTGG ACTCGCAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTCGC CTGGTCACCT GATAATTCCC CCGTGCGGCT GAAGATGCTA 
TATGCAGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGAG GCGGCCACAT CAAGGATGAG 
CTGTTTGGGA CTGTGAAGGA TGACCTCTCC TTTGCCGGGT ACCAGAAGCA CCTGTCGTCC 
TGTGCAGCGC CTGCCCCACT GACCTCGGCT GAAAGAGAAC TCCAGCAGAT CCGTATTAAC 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAACACCAGA CCTTGCAGGG CCTGGCCTTC 
CCCCTGCAGC CGCAGGCCCA ACGGGCACTT CAGCAGCTCA GACAGAAGAC GATCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACTATCGAGC TGGTGCACAC AGAGCCCACG 
GACGTGGCCC AGCTGCCGTC CCGAGTGCCC CGAGACGCCG CCCGCTACCA CTTCTTCCTC 
TACAAGCACA CCCACGAGGG TGACCCCCTG GAGTCTGTGG TGTTCATCTA CTCCATGCCC 
GGCTGCAAGT GCAGCATCAA GGAGCGCATG CTCTACTCCA GCTGCAAGAG CCGCCTCCTC 
GACTCCGTGG AGCAGGACTT CCAGCTGGAG ATCGCCAAGA AGATCGAGAT TGGTGATGGG 
GCGGAGCTGA CGGCTGACTT CCTCTACGAC GAGGTGCACC CCAAGCAGCA CCTCGCCAAA 
AGGGCCTTCG CCAAGCCCAG GGGCCCCGAG GGCAAGCGGG GCCACAAGCG CCTCATCCGT 
GGCCCCGGTG AGAATGGGGA TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010850050.1
CDS	138..1187
Translation	MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGAFRELVGCWDQDYDGAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPQAQRALQQLRQKTINYIQLKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGCKCSIKERMLYSSCKSRLLDSVEQDFQLEIAKKIEIGDGAELTADFLYDEVHPKQHLAKRAFAKPRGPEGKRGHKRLIRGPGENGDDS

Target ORF information:

RefSeq Version	XM_010851748.1
Organism	Bison bison bison
Definition	Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010851748.1

ORF Insert Sequence:

ATGGCGCATC AGACCGGCAT CCACGCCACT GAAGAGCTGA AGGAGTTCTT TGCCAAGGCG 
CGGGCTGGCT CTGTGCGACT CATCAAAGTT GTCATTGAGG ACGAACAGCT CGTGCTGGGC 
GCCTTTCGGG AGCTGGTGGG CTGCTGGGAC CAGGACTATG ACGGGGCCGT TCTGCCGCTG 
CTGGATGCCC AACAGCCTTG CTACCTGCTC TACCGCCTGG ACTCGCAGAA TGCCCAGGGC 
TTCGAGTGGC TCTTCCTCGC CTGGTCACCT GATAATTCCC CCGTGCGGCT GAAGATGCTA 
TATGCAGCCA CACGGGCCAC AGTGAAGAAG GAGTTTGGAG GCGGCCACAT CAAGGATGAG 
CTGTTTGGGA CTGTGAAGGA TGACCTCTCC TTTGCCGGGT ACCAGAAGCA CCTGTCGTCC 
TGTGCAGCGC CTGCCCCACT GACCTCGGCT GAAAGAGAAC TCCAGCAGAT CCGTATTAAC 
GAGGTGAAGA CAGAGATCAG CGTGGAGAGC AAACACCAGA CCTTGCAGGG CCTGGCCTTC 
CCCCTGCAGC CGCAGGCCCA ACGGGCACTT CAGCAGCTCA GACAGAAGAC GATCAACTAC 
ATCCAGCTGA AGCTGGACCT GGAGCGGGAG ACTATCGAGC TGGTGCACAC AGAGCCCACG 
GACGTGGCCC AGCTGCCGTC CCGAGTGCCC CGAGACGCCG CCCGCTACCA CTTCTTCCTC 
TACAAGCACA CCCACGAGGG TGACCCCCTG GAGTCTGTGG TGTTCATCTA CTCCATGCCC 
GGCTGCAAGT GCAGCATCAA GGAGCGCATG CTCTACTCCA GCTGCAAGAG CCGCCTCCTC 
GACTCCGTGG AGCAGGACTT CCAGCTGGAG ATCGCCAAGA AGATCGAGAT TGGTGATGGG 
GCGGAGCTGA CGGCTGACTT CCTCTACGAC GAGGTGCACC CCAAGCAGCA CCTCGCCAAA 
AGGGCCTTCG CCAAGCCCAG GGGCCCCGAG GGCAAGCGGG GCCACAAGCG CCTCATCCGT 
GGCCCCGGTG AGAATGGGGA TGACAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TWF2 ( XM_010851749.1 ) cDNA ORF clone, Bison bison bison -> XP_010850051.1 Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi73532
Clone ID Related Accession (Same CDS sequence)	XM_010851749.1
Accession Version	XM_010851749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	twinfilin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494876.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAGG AGAAGCCGGA GCCCTATTCA GGGGGACTGC CCTCGCTGTG GGGAGAAGGA 
ACTTGTGTCG GTCTCTTTGA GAGCAGGACC AAGGAATTCC AGCTCGTGCT GGGCGCCTTT 
CGGGAGCTGG TGGGCTGCTG GGACCAGGAC TATGACGGGG CCGTTCTGCC GCTGCTGGAT 
GCCCAACAGC CTTGCTACCT GCTCTACCGC CTGGACTCGC AGAATGCCCA GGGCTTCGAG 
TGGCTCTTCC TCGCCTGGTC ACCTGATAAT TCCCCCGTGC GGCTGAAGAT GCTATATGCA 
GCCACACGGG CCACAGTGAA GAAGGAGTTT GGAGGCGGCC ACATCAAGGA TGAGCTGTTT 
GGGACTGTGA AGGATGACCT CTCCTTTGCC GGGTACCAGA AGCACCTGTC GTCCTGTGCA 
GCGCCTGCCC CACTGACCTC GGCTGAAAGA GAACTCCAGC AGATCCGTAT TAACGAGGTG 
AAGACAGAGA TCAGCGTGGA GAGCAAACAC CAGACCTTGC AGGGCCTGGC CTTCCCCCTG 
CAGCCGCAGG CCCAACGGGC ACTTCAGCAG CTCAGACAGA AGACGATCAA CTACATCCAG 
CTGAAGCTGG ACCTGGAGCG GGAGACTATC GAGCTGGTGC ACACAGAGCC CACGGACGTG 
GCCCAGCTGC CGTCCCGAGT GCCCCGAGAC GCCGCCCGCT ACCACTTCTT CCTCTACAAG 
CACACCCACG AGGGTGACCC CCTGGAGTCT GTGGTGTTCA TCTACTCCAT GCCCGGCTGC 
AAGTGCAGCA TCAAGGAGCG CATGCTCTAC TCCAGCTGCA AGAGCCGCCT CCTCGACTCC 
GTGGAGCAGG ACTTCCAGCT GGAGATCGCC AAGAAGATCG AGATTGGTGA TGGGGCGGAG 
CTGACGGCTG ACTTCCTCTA CGACGAGGTG CACCCCAAGC AGCACCTCGC CAAAAGGGCC 
TTCGCCAAGC CCAGGGGCCC CGAGGGCAAG CGGGGCCACA AGCGCCTCAT CCGTGGCCCC 
GGTGAGAATG GGGATGACAG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010850051.1
CDS	373..1416
Translation	MPKEKPEPYSGGLPSLWGEGTCVGLFESRTKEFQLVLGAFRELVGCWDQDYDGAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPQAQRALQQLRQKTINYIQLKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGCKCSIKERMLYSSCKSRLLDSVEQDFQLEIAKKIEIGDGAELTADFLYDEVHPKQHLAKRAFAKPRGPEGKRGHKRLIRGPGENGDDS

Target ORF information:

RefSeq Version	XM_010851749.1
Organism	Bison bison bison
Definition	Bison bison bison twinfilin actin-binding protein 2 (TWF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010851749.1

ORF Insert Sequence:

ATGCCTAAGG AGAAGCCGGA GCCCTATTCA GGGGGACTGC CCTCGCTGTG GGGAGAAGGA 
ACTTGTGTCG GTCTCTTTGA GAGCAGGACC AAGGAATTCC AGCTCGTGCT GGGCGCCTTT 
CGGGAGCTGG TGGGCTGCTG GGACCAGGAC TATGACGGGG CCGTTCTGCC GCTGCTGGAT 
GCCCAACAGC CTTGCTACCT GCTCTACCGC CTGGACTCGC AGAATGCCCA GGGCTTCGAG 
TGGCTCTTCC TCGCCTGGTC ACCTGATAAT TCCCCCGTGC GGCTGAAGAT GCTATATGCA 
GCCACACGGG CCACAGTGAA GAAGGAGTTT GGAGGCGGCC ACATCAAGGA TGAGCTGTTT 
GGGACTGTGA AGGATGACCT CTCCTTTGCC GGGTACCAGA AGCACCTGTC GTCCTGTGCA 
GCGCCTGCCC CACTGACCTC GGCTGAAAGA GAACTCCAGC AGATCCGTAT TAACGAGGTG 
AAGACAGAGA TCAGCGTGGA GAGCAAACAC CAGACCTTGC AGGGCCTGGC CTTCCCCCTG 
CAGCCGCAGG CCCAACGGGC ACTTCAGCAG CTCAGACAGA AGACGATCAA CTACATCCAG 
CTGAAGCTGG ACCTGGAGCG GGAGACTATC GAGCTGGTGC ACACAGAGCC CACGGACGTG 
GCCCAGCTGC CGTCCCGAGT GCCCCGAGAC GCCGCCCGCT ACCACTTCTT CCTCTACAAG 
CACACCCACG AGGGTGACCC CCTGGAGTCT GTGGTGTTCA TCTACTCCAT GCCCGGCTGC 
AAGTGCAGCA TCAAGGAGCG CATGCTCTAC TCCAGCTGCA AGAGCCGCCT CCTCGACTCC 
GTGGAGCAGG ACTTCCAGCT GGAGATCGCC AAGAAGATCG AGATTGGTGA TGGGGCGGAG 
CTGACGGCTG ACTTCCTCTA CGACGAGGTG CACCCCAAGC AGCACCTCGC CAAAAGGGCC 
TTCGCCAAGC CCAGGGGCCC CGAGGGCAAG CGGGGCCACA AGCGCCTCAT CCGTGGCCCC 
GGTGAGAATG GGGATGACAG CTAG