EPHA3 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	EPHA3
Entrez Gene ID	104994380
Full Name	EPH receptor A3
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010847913.1	XP_010846215.1	ephrin type-A receptor 3 isoform X2
XM_010847912.1	XP_010846214.1	ephrin type-A receptor 3 isoform X1

Pathways from BioSystems
Gene Ontology

The following EPHA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi70693	XM_010847912.1 Latest version!	Bison bison bison EPH receptor A3 (EPHA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBi70694	XM_010847913.1 Latest version!	Bison bison bison EPH receptor A3 (EPHA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPHA3 ( XM_010847912.1 ) cDNA ORF clone, Bison bison bison -> XP_010846214.1 Bison bison bison EPH receptor A3 (EPHA3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi70693
Clone ID Related Accession (Same CDS sequence)	XM_010847912.1
Accession Version	XM_010847912.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	ephrin type-A receptor 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494830.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC ACCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGCTCTCAA GTGCTTCGGG 
GAACTGACTC TCCAACCTTC CAATGAAGTC AATTTACTGG ATTCAAAAAC CATTCAAGGG 
GAGCTGGGAT GGATCTCCTA TCCGTCACAT GGGTGGGAAG AAATCAGTGG TGTTGATGAA 
CATTACACAC CCATCCGGAC TTACCAGGTG TGCAATGTCA TGGATCATAG TCAAAATAAT 
TGGCTGAGGA CAAACTGGAT CCCCAGGAAC TCAGCTCAGA AGATCTATGT GGAGCTAAAG 
TTCACTCTCC GAGACTGCAA TAGCATTCCA TTGGTTTTGG GAACTTGCAA GGAGACTTTC 
AATCTGTACT ACATGGAGTC TGATGATGAT CATGGAGTCA AATTCCGAGA ACATCAGTTT 
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACGC AAATGGATCT GGGGGACCGT 
ATTCTTAAAC TCAACACTGA GGTTAGAGAA GTAGGTCCTG TCAACAAAAA GGGATTTTAT 
TTGGCTTTTC AAGATGTTGG TGCTTGTGTT GCCTTAGTGT CTGTGAGAGT GTACTTCAAA 
AAGTGCCCGT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACAGTACC AATGGATTCC 
CAATCTCTGG TGGAAGTTAG AGGTTCTTGT GTTAACAATT CTAAGGAGGA AGATCCTCCT 
AGGATGTACT GCAGTACAGA AGGTGAATGG CTTGTACCCA TTGGCAAGTG CTCCTGCAAT 
GCTGGCTATG AAGAAAGAGG TTTCATGTGT CAAGCTTGTC AACCAGGTTT CTACAAGGCT 
TCAGATGGTA ATATGAAGTG TGCTAAGTGT CCACCTCACA GTTCTACTCA CGAAGACGGT 
TCAGTGAACT GCAGGTGTGA AAATAATTAC TTCCGAGCAG ACAAAGACCC TCCATCCATG 
GCTTGTACCC GACCTCCGTC TGCACCGAGA AATGTGATAT CTAATATAAA CGAGACCTCG 
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC 
ATCATATGCA AAAAATGTGG GTGGAATGTC AAACAGTGTG AGCCGTGCAG TCCAAATGTC 
CGCTTCCTCC CTCGGCAGTT TGGGCTCACC AACACCACGG TAACAGTGAC AGACCTCCTG 
GCACACACTA ACTACACCTT TGAGATCGAT GCCGTTAATG GGGTGTCGGA GCTGAGCTCA 
CCACCCAGAC AGTTTGCTGC AGTCAGCATC ACAACTAACC AGGCTGCTCC ATCACCTGTC 
ATGACGATTA AGAAAGACCG GACATCCAGA AACAGCATTT CTTTATCCTG GCAAGAACCT 
GAACACCCTA ATGGGATCAT ATTGGACTAT GAGGTCAAAT ACTATGAAAA GCAGGAACAA 
GAAACAAGTT ATACGATTCT CAGGGCACGA GGCACTAATG TTACCATCAG TAGCCTCAAG 
CCTGATACTA CATATGTATT CCAAATCCGA GCCCGGACCG CAGCTGGATA TGGGACCAAC 
AGTCGCAAGT TTGAGTTTGA AACCAGTCCA GACTCTTTCT CCATCTCTGG TGAAAATAGC 
CAAGTGGTAA TGATTGCCAT TTCAGCGGCG GTAGCAATTA TCCTCCTCAC AGTCGTCACC 
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT CATAAGTCGA AACATGGTGC AGATGAAAAA 
AGACTTCATT TTGGGAATGG ACACTTAAAA CTTCCAGGTC TCAGGACTTA TGTTGACCCA 
CACACATATG AGGACCCTAC CCAAGCAGTT CATGAGTTTG CTAAGGAATT GGATGCCACC 
AACATATCCA TTGACAAAGT TGTTGGAGCA GGTGAATTTG GAGAGGTGTG CAGTGGCCGT 
TTAAAACTGC CTTCAAAAAA AGAGATTTCA GTGGCCATCA AGACCCTAAA AGTGGGCTAC 
ACAGAAAAGC AGAGGAGAGA CTTCTTAGGC GAAGCAAGCA TTATGGGACA ATTTGACCAT 
CCCAATATCA TTCGACTGGA GGGAGTTGTT ACTAAAAGTA AACCTGTTAT GATTGTCACA 
GAATACATGG AGAATGGTTC CTTGGACAGT TTCTTACGCA AACATGATGC CCAGTTTACA 
GTTATCCAGC TGGTAGGGAT GCTTCGAGGC ATAGCATCTG GCATGAAGTA TCTCTCAGAC 
ATGGGCTATG TTCACCGAGA CCTTGCTGCT CGCAACATCT TGATCAACAG TAATTTGGTG 
TGTAAGGTTT CTGATTTTGG ACTTTCACGG GTTCTAGAAG ATGACCCAGA AGCTGCTTAC 
ACAACAAGAG GAGGGAAGAT CCCAATCCGG TGGACATCAC CAGAAGCCAT AGCCTACCGC 
AAATTCACAT CAGCCAGCGA TGTATGGAGT TACGGGATTG TTCTCTGGGA GGTGATGTCC 
TATGGAGAGA GACCATACTG GGAGATGTCC AATCAGGATG TAATTAAAGC TGTGGATGAA 
GGCTATCGAC TGCCACCCCC CATGGACTGC CCAGCTGCGT TGTATCAGCT GATGCTAGAC 
TGCTGGCAGA AAGACAGAAA CAACAGACCC AAGTTTGAGC AGATTGTCAG CATTCTGGAC 
AAGCTTATCC GGAACCCAGG CAGTCTGAAG ATCATCACCA GTGCGGCAGC AAGGCCCTCA 
AACCTTCTCC TGGACCAAAG TAACATCGAC ATTACTACCT TCCGCACGAC AGGTGACTGG 
CTTAACGGTG TCCGAACAGC ACAGTGCAAG GAAATCTTCA CAGGCGTGGA GTACAGTTCT 
TGTGACACCA TAGCCAAGAT TTCTGCAGAC GACATGAAAA AGGTCGGTGT CACTGTGGTT 
GGGCCACAGA AGAAGATCAT CAGCAGTATT AAAGTTCTAG AAACACAATC CAAGAATAGT 
CCAGTCCCAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010846214.1
CDS	112..3066
Translation	MDCHLSILLLLSCSALKCFGELTLQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWIPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEVREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACQPGFYKASDGNMKCAKCPPHSSTHEDGSVNCRCENNYFRADKDPPSMACTRPPSAPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNVKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVMTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTTYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGENSQVVMIAISAAVAIILLTVVTYVLIGRFCGYHKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNIDITTFRTTGDWLNGVRTAQCKEIFTGVEYSSCDTIAKISADDMKKVGVTVVGPQKKIISSIKVLETQSKNSPVPV

Target ORF information:

RefSeq Version	XM_010847912.1
Organism	Bison bison bison
Definition	Bison bison bison EPH receptor A3 (EPHA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010847912.1

ORF Insert Sequence:

ATGGATTGTC ACCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGCTCTCAA GTGCTTCGGG 
GAACTGACTC TCCAACCTTC CAATGAAGTC AATTTACTGG ATTCAAAAAC CATTCAAGGG 
GAGCTGGGAT GGATCTCCTA TCCGTCACAT GGGTGGGAAG AAATCAGTGG TGTTGATGAA 
CATTACACAC CCATCCGGAC TTACCAGGTG TGCAATGTCA TGGATCATAG TCAAAATAAT 
TGGCTGAGGA CAAACTGGAT CCCCAGGAAC TCAGCTCAGA AGATCTATGT GGAGCTAAAG 
TTCACTCTCC GAGACTGCAA TAGCATTCCA TTGGTTTTGG GAACTTGCAA GGAGACTTTC 
AATCTGTACT ACATGGAGTC TGATGATGAT CATGGAGTCA AATTCCGAGA ACATCAGTTT 
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACGC AAATGGATCT GGGGGACCGT 
ATTCTTAAAC TCAACACTGA GGTTAGAGAA GTAGGTCCTG TCAACAAAAA GGGATTTTAT 
TTGGCTTTTC AAGATGTTGG TGCTTGTGTT GCCTTAGTGT CTGTGAGAGT GTACTTCAAA 
AAGTGCCCGT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACAGTACC AATGGATTCC 
CAATCTCTGG TGGAAGTTAG AGGTTCTTGT GTTAACAATT CTAAGGAGGA AGATCCTCCT 
AGGATGTACT GCAGTACAGA AGGTGAATGG CTTGTACCCA TTGGCAAGTG CTCCTGCAAT 
GCTGGCTATG AAGAAAGAGG TTTCATGTGT CAAGCTTGTC AACCAGGTTT CTACAAGGCT 
TCAGATGGTA ATATGAAGTG TGCTAAGTGT CCACCTCACA GTTCTACTCA CGAAGACGGT 
TCAGTGAACT GCAGGTGTGA AAATAATTAC TTCCGAGCAG ACAAAGACCC TCCATCCATG 
GCTTGTACCC GACCTCCGTC TGCACCGAGA AATGTGATAT CTAATATAAA CGAGACCTCG 
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC 
ATCATATGCA AAAAATGTGG GTGGAATGTC AAACAGTGTG AGCCGTGCAG TCCAAATGTC 
CGCTTCCTCC CTCGGCAGTT TGGGCTCACC AACACCACGG TAACAGTGAC AGACCTCCTG 
GCACACACTA ACTACACCTT TGAGATCGAT GCCGTTAATG GGGTGTCGGA GCTGAGCTCA 
CCACCCAGAC AGTTTGCTGC AGTCAGCATC ACAACTAACC AGGCTGCTCC ATCACCTGTC 
ATGACGATTA AGAAAGACCG GACATCCAGA AACAGCATTT CTTTATCCTG GCAAGAACCT 
GAACACCCTA ATGGGATCAT ATTGGACTAT GAGGTCAAAT ACTATGAAAA GCAGGAACAA 
GAAACAAGTT ATACGATTCT CAGGGCACGA GGCACTAATG TTACCATCAG TAGCCTCAAG 
CCTGATACTA CATATGTATT CCAAATCCGA GCCCGGACCG CAGCTGGATA TGGGACCAAC 
AGTCGCAAGT TTGAGTTTGA AACCAGTCCA GACTCTTTCT CCATCTCTGG TGAAAATAGC 
CAAGTGGTAA TGATTGCCAT TTCAGCGGCG GTAGCAATTA TCCTCCTCAC AGTCGTCACC 
TATGTTTTGA TTGGGAGGTT CTGTGGCTAT CATAAGTCGA AACATGGTGC AGATGAAAAA 
AGACTTCATT TTGGGAATGG ACACTTAAAA CTTCCAGGTC TCAGGACTTA TGTTGACCCA 
CACACATATG AGGACCCTAC CCAAGCAGTT CATGAGTTTG CTAAGGAATT GGATGCCACC 
AACATATCCA TTGACAAAGT TGTTGGAGCA GGTGAATTTG GAGAGGTGTG CAGTGGCCGT 
TTAAAACTGC CTTCAAAAAA AGAGATTTCA GTGGCCATCA AGACCCTAAA AGTGGGCTAC 
ACAGAAAAGC AGAGGAGAGA CTTCTTAGGC GAAGCAAGCA TTATGGGACA ATTTGACCAT 
CCCAATATCA TTCGACTGGA GGGAGTTGTT ACTAAAAGTA AACCTGTTAT GATTGTCACA 
GAATACATGG AGAATGGTTC CTTGGACAGT TTCTTACGCA AACATGATGC CCAGTTTACA 
GTTATCCAGC TGGTAGGGAT GCTTCGAGGC ATAGCATCTG GCATGAAGTA TCTCTCAGAC 
ATGGGCTATG TTCACCGAGA CCTTGCTGCT CGCAACATCT TGATCAACAG TAATTTGGTG 
TGTAAGGTTT CTGATTTTGG ACTTTCACGG GTTCTAGAAG ATGACCCAGA AGCTGCTTAC 
ACAACAAGAG GAGGGAAGAT CCCAATCCGG TGGACATCAC CAGAAGCCAT AGCCTACCGC 
AAATTCACAT CAGCCAGCGA TGTATGGAGT TACGGGATTG TTCTCTGGGA GGTGATGTCC 
TATGGAGAGA GACCATACTG GGAGATGTCC AATCAGGATG TAATTAAAGC TGTGGATGAA 
GGCTATCGAC TGCCACCCCC CATGGACTGC CCAGCTGCGT TGTATCAGCT GATGCTAGAC 
TGCTGGCAGA AAGACAGAAA CAACAGACCC AAGTTTGAGC AGATTGTCAG CATTCTGGAC 
AAGCTTATCC GGAACCCAGG CAGTCTGAAG ATCATCACCA GTGCGGCAGC AAGGCCCTCA 
AACCTTCTCC TGGACCAAAG TAACATCGAC ATTACTACCT TCCGCACGAC AGGTGACTGG 
CTTAACGGTG TCCGAACAGC ACAGTGCAAG GAAATCTTCA CAGGCGTGGA GTACAGTTCT 
TGTGACACCA TAGCCAAGAT TTCTGCAGAC GACATGAAAA AGGTCGGTGT CACTGTGGTT 
GGGCCACAGA AGAAGATCAT CAGCAGTATT AAAGTTCTAG AAACACAATC CAAGAATAGT 
CCAGTCCCAG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHA3 ( XM_010847913.1 ) cDNA ORF clone, Bison bison bison -> XP_010846215.1 Bison bison bison EPH receptor A3 (EPHA3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi70694
Clone ID Related Accession (Same CDS sequence)	XM_010847913.1
Accession Version	XM_010847913.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	ephrin type-A receptor 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494830.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTGTC ACCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGCTCTCAA GTGCTTCGGG 
GAACTGACTC TCCAACCTTC CAATGAAGTC AATTTACTGG ATTCAAAAAC CATTCAAGGG 
GAGCTGGGAT GGATCTCCTA TCCGTCACAT GGGTGGGAAG AAATCAGTGG TGTTGATGAA 
CATTACACAC CCATCCGGAC TTACCAGGTG TGCAATGTCA TGGATCATAG TCAAAATAAT 
TGGCTGAGGA CAAACTGGAT CCCCAGGAAC TCAGCTCAGA AGATCTATGT GGAGCTAAAG 
TTCACTCTCC GAGACTGCAA TAGCATTCCA TTGGTTTTGG GAACTTGCAA GGAGACTTTC 
AATCTGTACT ACATGGAGTC TGATGATGAT CATGGAGTCA AATTCCGAGA ACATCAGTTT 
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACGC AAATGGATCT GGGGGACCGT 
ATTCTTAAAC TCAACACTGA GGTTAGAGAA GTAGGTCCTG TCAACAAAAA GGGATTTTAT 
TTGGCTTTTC AAGATGTTGG TGCTTGTGTT GCCTTAGTGT CTGTGAGAGT GTACTTCAAA 
AAGTGCCCGT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACAGTACC AATGGATTCC 
CAATCTCTGG TGGAAGTTAG AGGTTCTTGT GTTAACAATT CTAAGGAGGA AGATCCTCCT 
AGGATGTACT GCAGTACAGA AGGTGAATGG CTTGTACCCA TTGGCAAGTG CTCCTGCAAT 
GCTGGCTATG AAGAAAGAGG TTTCATGTGT CAAGCTTGTC AACCAGGTTT CTACAAGGCT 
TCAGATGGTA ATATGAAGTG TGCTAAGTGT CCACCTCACA GTTCTACTCA CGAAGACGGT 
TCAGTGAACT GCAGGTGTGA AAATAATTAC TTCCGAGCAG ACAAAGACCC TCCATCCATG 
GCTTGTACCC GACCTCCGTC TGCACCGAGA AATGTGATAT CTAATATAAA CGAGACCTCG 
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC 
ATCATATGCA AAAAATGTGG GTGGAATGTC AAACAGTGTG AGCCGTGCAG TCCAAATGTC 
CGCTTCCTCC CTCGGCAGTT TGGGCTCACC AACACCACGG TAACAGTGAC AGACCTCCTG 
GCACACACTA ACTACACCTT TGAGATCGAT GCCGTTAATG GGGTGTCGGA GCTGAGCTCA 
CCACCCAGAC AGTTTGCTGC AGTCAGCATC ACAACTAACC AGGCTGCTCC ATCACCTGTC 
ATGACGATTA AGAAAGACCG GACATCCAGA AACAGCATTT CTTTATCCTG GCAAGAACCT 
GAACACCCTA ATGGGATCAT ATTGGACTAT GAGGTCAAAT ACTATGAAAA GGAACAAGAA 
ACAAGTTATA CGATTCTCAG GGCACGAGGC ACTAATGTTA CCATCAGTAG CCTCAAGCCT 
GATACTACAT ATGTATTCCA AATCCGAGCC CGGACCGCAG CTGGATATGG GACCAACAGT 
CGCAAGTTTG AGTTTGAAAC CAGTCCAGAC TCTTTCTCCA TCTCTGGTGA AAATAGCCAA 
GTGGTAATGA TTGCCATTTC AGCGGCGGTA GCAATTATCC TCCTCACAGT CGTCACCTAT 
GTTTTGATTG GGAGGTTCTG TGGCTATCAT AAGTCGAAAC ATGGTGCAGA TGAAAAAAGA 
CTTCATTTTG GGAATGGACA CTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAC 
ACATATGAGG ACCCTACCCA AGCAGTTCAT GAGTTTGCTA AGGAATTGGA TGCCACCAAC 
ATATCCATTG ACAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGCCGTTTA 
AAACTGCCTT CAAAAAAAGA GATTTCAGTG GCCATCAAGA CCCTAAAAGT GGGCTACACA 
GAAAAGCAGA GGAGAGACTT CTTAGGCGAA GCAAGCATTA TGGGACAATT TGACCATCCC 
AATATCATTC GACTGGAGGG AGTTGTTACT AAAAGTAAAC CTGTTATGAT TGTCACAGAA 
TACATGGAGA ATGGTTCCTT GGACAGTTTC TTACGCAAAC ATGATGCCCA GTTTACAGTT 
ATCCAGCTGG TAGGGATGCT TCGAGGCATA GCATCTGGCA TGAAGTATCT CTCAGACATG 
GGCTATGTTC ACCGAGACCT TGCTGCTCGC AACATCTTGA TCAACAGTAA TTTGGTGTGT 
AAGGTTTCTG ATTTTGGACT TTCACGGGTT CTAGAAGATG ACCCAGAAGC TGCTTACACA 
ACAAGAGGAG GGAAGATCCC AATCCGGTGG ACATCACCAG AAGCCATAGC CTACCGCAAA 
TTCACATCAG CCAGCGATGT ATGGAGTTAC GGGATTGTTC TCTGGGAGGT GATGTCCTAT 
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT GGATGAAGGC 
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCGTTGT ATCAGCTGAT GCTAGACTGC 
TGGCAGAAAG ACAGAAACAA CAGACCCAAG TTTGAGCAGA TTGTCAGCAT TCTGGACAAG 
CTTATCCGGA ACCCAGGCAG TCTGAAGATC ATCACCAGTG CGGCAGCAAG GCCCTCAAAC 
CTTCTCCTGG ACCAAAGTAA CATCGACATT ACTACCTTCC GCACGACAGG TGACTGGCTT 
AACGGTGTCC GAACAGCACA GTGCAAGGAA ATCTTCACAG GCGTGGAGTA CAGTTCTTGT 
GACACCATAG CCAAGATTTC TGCAGACGAC ATGAAAAAGG TCGGTGTCAC TGTGGTTGGG 
CCACAGAAGA AGATCATCAG CAGTATTAAA GTTCTAGAAA CACAATCCAA GAATAGTCCA 
GTCCCAGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010846215.1
CDS	112..3063
Translation	MDCHLSILLLLSCSALKCFGELTLQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWIPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEVREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACQPGFYKASDGNMKCAKCPPHSSTHEDGSVNCRCENNYFRADKDPPSMACTRPPSAPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNVKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVMTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKEQETSYTILRARGTNVTISSLKPDTTYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGENSQVVMIAISAAVAIILLTVVTYVLIGRFCGYHKSKHGADEKRLHFGNGHLKLPGLRTYVDPHTYEDPTQAVHEFAKELDATNISIDKVVGAGEFGEVCSGRLKLPSKKEISVAIKTLKVGYTEKQRRDFLGEASIMGQFDHPNIIRLEGVVTKSKPVMIVTEYMENGSLDSFLRKHDAQFTVIQLVGMLRGIASGMKYLSDMGYVHRDLAARNILINSNLVCKVSDFGLSRVLEDDPEAAYTTRGGKIPIRWTSPEAIAYRKFTSASDVWSYGIVLWEVMSYGERPYWEMSNQDVIKAVDEGYRLPPPMDCPAALYQLMLDCWQKDRNNRPKFEQIVSILDKLIRNPGSLKIITSAAARPSNLLLDQSNIDITTFRTTGDWLNGVRTAQCKEIFTGVEYSSCDTIAKISADDMKKVGVTVVGPQKKIISSIKVLETQSKNSPVPV

Target ORF information:

RefSeq Version	XM_010847913.1
Organism	Bison bison bison
Definition	Bison bison bison EPH receptor A3 (EPHA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010847913.1

ORF Insert Sequence:

ATGGATTGTC ACCTCTCCAT CCTCCTCCTT CTCAGCTGCT CTGCTCTCAA GTGCTTCGGG 
GAACTGACTC TCCAACCTTC CAATGAAGTC AATTTACTGG ATTCAAAAAC CATTCAAGGG 
GAGCTGGGAT GGATCTCCTA TCCGTCACAT GGGTGGGAAG AAATCAGTGG TGTTGATGAA 
CATTACACAC CCATCCGGAC TTACCAGGTG TGCAATGTCA TGGATCATAG TCAAAATAAT 
TGGCTGAGGA CAAACTGGAT CCCCAGGAAC TCAGCTCAGA AGATCTATGT GGAGCTAAAG 
TTCACTCTCC GAGACTGCAA TAGCATTCCA TTGGTTTTGG GAACTTGCAA GGAGACTTTC 
AATCTGTACT ACATGGAGTC TGATGATGAT CATGGAGTCA AATTCCGAGA ACATCAGTTT 
ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACGC AAATGGATCT GGGGGACCGT 
ATTCTTAAAC TCAACACTGA GGTTAGAGAA GTAGGTCCTG TCAACAAAAA GGGATTTTAT 
TTGGCTTTTC AAGATGTTGG TGCTTGTGTT GCCTTAGTGT CTGTGAGAGT GTACTTCAAA 
AAGTGCCCGT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACAGTACC AATGGATTCC 
CAATCTCTGG TGGAAGTTAG AGGTTCTTGT GTTAACAATT CTAAGGAGGA AGATCCTCCT 
AGGATGTACT GCAGTACAGA AGGTGAATGG CTTGTACCCA TTGGCAAGTG CTCCTGCAAT 
GCTGGCTATG AAGAAAGAGG TTTCATGTGT CAAGCTTGTC AACCAGGTTT CTACAAGGCT 
TCAGATGGTA ATATGAAGTG TGCTAAGTGT CCACCTCACA GTTCTACTCA CGAAGACGGT 
TCAGTGAACT GCAGGTGTGA AAATAATTAC TTCCGAGCAG ACAAAGACCC TCCATCCATG 
GCTTGTACCC GACCTCCGTC TGCACCGAGA AATGTGATAT CTAATATAAA CGAGACCTCG 
GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC 
ATCATATGCA AAAAATGTGG GTGGAATGTC AAACAGTGTG AGCCGTGCAG TCCAAATGTC 
CGCTTCCTCC CTCGGCAGTT TGGGCTCACC AACACCACGG TAACAGTGAC AGACCTCCTG 
GCACACACTA ACTACACCTT TGAGATCGAT GCCGTTAATG GGGTGTCGGA GCTGAGCTCA 
CCACCCAGAC AGTTTGCTGC AGTCAGCATC ACAACTAACC AGGCTGCTCC ATCACCTGTC 
ATGACGATTA AGAAAGACCG GACATCCAGA AACAGCATTT CTTTATCCTG GCAAGAACCT 
GAACACCCTA ATGGGATCAT ATTGGACTAT GAGGTCAAAT ACTATGAAAA GGAACAAGAA 
ACAAGTTATA CGATTCTCAG GGCACGAGGC ACTAATGTTA CCATCAGTAG CCTCAAGCCT 
GATACTACAT ATGTATTCCA AATCCGAGCC CGGACCGCAG CTGGATATGG GACCAACAGT 
CGCAAGTTTG AGTTTGAAAC CAGTCCAGAC TCTTTCTCCA TCTCTGGTGA AAATAGCCAA 
GTGGTAATGA TTGCCATTTC AGCGGCGGTA GCAATTATCC TCCTCACAGT CGTCACCTAT 
GTTTTGATTG GGAGGTTCTG TGGCTATCAT AAGTCGAAAC ATGGTGCAGA TGAAAAAAGA 
CTTCATTTTG GGAATGGACA CTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAC 
ACATATGAGG ACCCTACCCA AGCAGTTCAT GAGTTTGCTA AGGAATTGGA TGCCACCAAC 
ATATCCATTG ACAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGCCGTTTA 
AAACTGCCTT CAAAAAAAGA GATTTCAGTG GCCATCAAGA CCCTAAAAGT GGGCTACACA 
GAAAAGCAGA GGAGAGACTT CTTAGGCGAA GCAAGCATTA TGGGACAATT TGACCATCCC 
AATATCATTC GACTGGAGGG AGTTGTTACT AAAAGTAAAC CTGTTATGAT TGTCACAGAA 
TACATGGAGA ATGGTTCCTT GGACAGTTTC TTACGCAAAC ATGATGCCCA GTTTACAGTT 
ATCCAGCTGG TAGGGATGCT TCGAGGCATA GCATCTGGCA TGAAGTATCT CTCAGACATG 
GGCTATGTTC ACCGAGACCT TGCTGCTCGC AACATCTTGA TCAACAGTAA TTTGGTGTGT 
AAGGTTTCTG ATTTTGGACT TTCACGGGTT CTAGAAGATG ACCCAGAAGC TGCTTACACA 
ACAAGAGGAG GGAAGATCCC AATCCGGTGG ACATCACCAG AAGCCATAGC CTACCGCAAA 
TTCACATCAG CCAGCGATGT ATGGAGTTAC GGGATTGTTC TCTGGGAGGT GATGTCCTAT 
GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT GGATGAAGGC 
TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCGTTGT ATCAGCTGAT GCTAGACTGC 
TGGCAGAAAG ACAGAAACAA CAGACCCAAG TTTGAGCAGA TTGTCAGCAT TCTGGACAAG 
CTTATCCGGA ACCCAGGCAG TCTGAAGATC ATCACCAGTG CGGCAGCAAG GCCCTCAAAC 
CTTCTCCTGG ACCAAAGTAA CATCGACATT ACTACCTTCC GCACGACAGG TGACTGGCTT 
AACGGTGTCC GAACAGCACA GTGCAAGGAA ATCTTCACAG GCGTGGAGTA CAGTTCTTGT 
GACACCATAG CCAAGATTTC TGCAGACGAC ATGAAAAAGG TCGGTGTCAC TGTGGTTGGG 
CCACAGAAGA AGATCATCAG CAGTATTAAA GTTCTAGAAA CACAATCCAA GAATAGTCCA 
GTCCCAGTGT AA