EZH1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	EZH1
Entrez Gene ID	104990428
Full Name	enhancer of zeste 1 polycomb repressive complex 2 subunit
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010842500.1	XP_010840802.1	histone-lysine N-methyltransferase EZH1

Pathways from BioSystems
Gene Ontology

The following EZH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EZH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi66656	XM_010842500.1 Latest version!	Bison bison bison enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EZH1 ( XM_010842500.1 ) cDNA ORF clone, Bison bison bison -> XP_010840802.1 Bison bison bison enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), mRNA.

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CloneID	OBi66656
Clone ID Related Accession (Same CDS sequence)	XM_010842500.1
Accession Version	XM_010842500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	histone-lysine N-methyltransferase EZH1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494752.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATAC CAAATCCCCC GACGTCCAAA TGTATCACTT ATTGGAAGAG GAAAGTTAAG 
TCTGAATACA TGCGACTTCG GCAGCTTAAA CGGCTTCAGG CGAATATGGG TGCAAAGGCT 
CTCTATGTAG CAAATTTTGC AAAAGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG 
AAGAAGCTTC GTGTCCAACC TGTTCAGTTA ATGAAGCCTG TGAGTGGGCA TCCTTTTCTC 
AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAGCACAT GTTAATGAGG 
TCTCTGAACA CAGTTGCCTT GGTTCCCATC ATGTACTCGT GGTCCCCTCT CCAGCAGAAC 
TTCATGGTGG AAGATGAGAC AGTTTTGTGC AATATCCCCT ACATGGGAGA TGAGGTGAAA 
GAGGAAGATG AGACTTTCAT TGAGGAACTG ATCAATAACT ACGATGGCAA AGTCCACGGT 
GAAGAAGAGA TGATTCCTGG CTCTGTCCTG ATCAGTGATG CTGTTTTCCT GGAGTTGGTA 
GATGCTCTGA ATCAATACTC GGATGAAGAT GAAGAAGGGC ACAACGACAC CTCAGATGGA 
AAGCAAGATG ACAGCAAAGA GGATCTGCCA GTAACAAGAA AGAGGAAACG ACATGCTATT 
GAAGGCAGCA AAAAGAGTTC CAAGAAACAA TTCCCAAATG ACATGATCTT CAGTGCGATC 
GCCTCGATGT TCCCTGAGAA CGGTGTCCCA GACGACATGA AGGAGAGGTA CCGAGAGCTC 
ACAGAGATGT CAGACCCCAA TGCGCTCCCC CCGCAGTGCA CACCCAACAT CGACGGCCCC 
AACGCCAAGT CTGTGCAGCG GGAACAGTCT CTGCACTCCT TCCACACACT CTTCTGCCGG 
CGCTGCTTCA AATACGACTG CTTCCTTCAC CCTTTCCATG CCACCCCTAA TGTGTATAAA 
CGCAAGAACA AAGAAATCAA GATTGAGCCA GAACCGTGTG GCACAGACTG CTTCCTTCTG 
CTGGAAGGAG CAAAGGAGTA CGCCATGCTG CACAACCCTC GCTCCAAGTG CTCTGGGCGT 
CGCCGGCGAA GGCACCACGT GGTCAATGCT TCCTGCTCCA ACACTTCAGC CTCTGCTGTG 
GCTGAGACCA AAGAAGGGGA CAGCGACAGG GACACTGGCA ATGACTGGGC CTCCAGTTCT 
TCAGAGGCAA ACTCTCGCTG TCAGACGCCC ACGAAACAGA AGGCGAGTCC AGCCCCACCT 
CAGCTCTGTG TCGTGGAAGC ACCCTCAGAA CCTGTGGAGT GGACTGGGGC TGAAGAATCT 
CTTTTTCGAG TCTTTCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGACTTCTG 
GGGACTAAGA CATGCAAGCA GGTCTTCCAG TTTGCAGTCA AAGAGTCACT TATCCTGAAG 
CTGCCCACAG ATGAGCTTAT GAACCCCTCG CAGAAGAAGA AGAGAAAGCA CAGGTTGTGG 
GCTGCACACT GCCGGAAAAT TCAGCTGAAG AAAGATAACT CCTCCACCCA GGTGTACAAC 
TACCAGCCCT GCGACCACCC GGACCGCCCC TGTGACAGCA CGTGCCCCTG CATCATGACT 
CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCCGACT GTCAGAATCG CTTTCCCGGC 
TGTCGCTGTA AGACCCAGTG CAATACCAAG CAGTGCCCGT GCTACCTGGC GGTGCGTGAG 
TGTGACCCTG ACCTGTGCCT CACCTGTGGG GCCTCGGAGC ACTGGGACTG CAAGGTGGTT 
TCCTGTAAGA ACTGCAGCAT TCAACGTGGC CTCAAAAAGC ACCTGCTGCT GGCCCCCTCA 
GATGTGGCCG GATGGGGCAC CTTCATTAAG GAGTCTGTGC AGAAGAATGA ATTCATTTCT 
GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCCGATC GACGAGGGAA GGTCTATGAC 
AAGTACATGT CCAGCTTCCT GTTCAACCTC AATAACGATT TTGTAGTGGA TGCTACCCGG 
AAAGGAAACA AAATTCGATT TGCAAACCAT TCGGTGAACC CCAACTGTTA TGCCAAAGTG 
GTCATGGTGA ATGGGGATCA TCGAATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGT 
GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CCCTCAAGTA CGTGGGGATC 
GAGCGGGAGA CGGATGTCCT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010840802.1
CDS	577..2820
Translation	MDIPNPPTSKCITYWKRKVKSEYMRLRQLKRLQANMGAKALYVANFAKVQEKTQILNEEWKKLRVQPVQLMKPVSGHPFLKKCTIESIFPGFASQHMLMRSLNTVALVPIMYSWSPLQQNFMVEDETVLCNIPYMGDEVKEEDETFIEELINNYDGKVHGEEEMIPGSVLISDAVFLELVDALNQYSDEDEEGHNDTSDGKQDDSKEDLPVTRKRKRHAIEGSKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNVYKRKNKEIKIEPEPCGTDCFLLLEGAKEYAMLHNPRSKCSGRRRRRHHVVNASCSNTSASAVAETKEGDSDRDTGNDWASSSSEANSRCQTPTKQKASPAPPQLCVVEAPSEPVEWTGAEESLFRVFHGTYFNNFCSIARLLGTKTCKQVFQFAVKESLILKLPTDELMNPSQKKKRKHRLWAAHCRKIQLKKDNSSTQVYNYQPCDHPDRPCDSTCPCIMTQNFCEKFCQCNPDCQNRFPGCRCKTQCNTKQCPCYLAVRECDPDLCLTCGASEHWDCKVVSCKNCSIQRGLKKHLLLAPSDVAGWGTFIKESVQKNEFISEYCGELISQDEADRRGKVYDKYMSSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVVMVNGDHRIGIFAKRAIQAGEELFFDYRYSQADALKYVGIERETDVL

Target ORF information:

RefSeq Version	XM_010842500.1
Organism	Bison bison bison
Definition	Bison bison bison enhancer of zeste 1 polycomb repressive complex 2 subunit (EZH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010842500.1

ORF Insert Sequence:

ATGGATATAC CAAATCCCCC GACGTCCAAA TGTATCACTT ATTGGAAGAG GAAAGTTAAG 
TCTGAATACA TGCGACTTCG GCAGCTTAAA CGGCTTCAGG CGAATATGGG TGCAAAGGCT 
CTCTATGTAG CAAATTTTGC AAAAGTTCAA GAAAAAACCC AGATCCTCAA TGAAGAATGG 
AAGAAGCTTC GTGTCCAACC TGTTCAGTTA ATGAAGCCTG TGAGTGGGCA TCCTTTTCTC 
AAAAAGTGTA CCATAGAGAG CATTTTCCCG GGATTTGCAA GCCAGCACAT GTTAATGAGG 
TCTCTGAACA CAGTTGCCTT GGTTCCCATC ATGTACTCGT GGTCCCCTCT CCAGCAGAAC 
TTCATGGTGG AAGATGAGAC AGTTTTGTGC AATATCCCCT ACATGGGAGA TGAGGTGAAA 
GAGGAAGATG AGACTTTCAT TGAGGAACTG ATCAATAACT ACGATGGCAA AGTCCACGGT 
GAAGAAGAGA TGATTCCTGG CTCTGTCCTG ATCAGTGATG CTGTTTTCCT GGAGTTGGTA 
GATGCTCTGA ATCAATACTC GGATGAAGAT GAAGAAGGGC ACAACGACAC CTCAGATGGA 
AAGCAAGATG ACAGCAAAGA GGATCTGCCA GTAACAAGAA AGAGGAAACG ACATGCTATT 
GAAGGCAGCA AAAAGAGTTC CAAGAAACAA TTCCCAAATG ACATGATCTT CAGTGCGATC 
GCCTCGATGT TCCCTGAGAA CGGTGTCCCA GACGACATGA AGGAGAGGTA CCGAGAGCTC 
ACAGAGATGT CAGACCCCAA TGCGCTCCCC CCGCAGTGCA CACCCAACAT CGACGGCCCC 
AACGCCAAGT CTGTGCAGCG GGAACAGTCT CTGCACTCCT TCCACACACT CTTCTGCCGG 
CGCTGCTTCA AATACGACTG CTTCCTTCAC CCTTTCCATG CCACCCCTAA TGTGTATAAA 
CGCAAGAACA AAGAAATCAA GATTGAGCCA GAACCGTGTG GCACAGACTG CTTCCTTCTG 
CTGGAAGGAG CAAAGGAGTA CGCCATGCTG CACAACCCTC GCTCCAAGTG CTCTGGGCGT 
CGCCGGCGAA GGCACCACGT GGTCAATGCT TCCTGCTCCA ACACTTCAGC CTCTGCTGTG 
GCTGAGACCA AAGAAGGGGA CAGCGACAGG GACACTGGCA ATGACTGGGC CTCCAGTTCT 
TCAGAGGCAA ACTCTCGCTG TCAGACGCCC ACGAAACAGA AGGCGAGTCC AGCCCCACCT 
CAGCTCTGTG TCGTGGAAGC ACCCTCAGAA CCTGTGGAGT GGACTGGGGC TGAAGAATCT 
CTTTTTCGAG TCTTTCATGG CACCTACTTC AACAACTTCT GTTCAATAGC CAGACTTCTG 
GGGACTAAGA CATGCAAGCA GGTCTTCCAG TTTGCAGTCA AAGAGTCACT TATCCTGAAG 
CTGCCCACAG ATGAGCTTAT GAACCCCTCG CAGAAGAAGA AGAGAAAGCA CAGGTTGTGG 
GCTGCACACT GCCGGAAAAT TCAGCTGAAG AAAGATAACT CCTCCACCCA GGTGTACAAC 
TACCAGCCCT GCGACCACCC GGACCGCCCC TGTGACAGCA CGTGCCCCTG CATCATGACT 
CAGAATTTCT GTGAGAAGTT CTGCCAGTGC AACCCCGACT GTCAGAATCG CTTTCCCGGC 
TGTCGCTGTA AGACCCAGTG CAATACCAAG CAGTGCCCGT GCTACCTGGC GGTGCGTGAG 
TGTGACCCTG ACCTGTGCCT CACCTGTGGG GCCTCGGAGC ACTGGGACTG CAAGGTGGTT 
TCCTGTAAGA ACTGCAGCAT TCAACGTGGC CTCAAAAAGC ACCTGCTGCT GGCCCCCTCA 
GATGTGGCCG GATGGGGCAC CTTCATTAAG GAGTCTGTGC AGAAGAATGA ATTCATTTCT 
GAATACTGTG GTGAGCTCAT CTCTCAGGAT GAGGCCGATC GACGAGGGAA GGTCTATGAC 
AAGTACATGT CCAGCTTCCT GTTCAACCTC AATAACGATT TTGTAGTGGA TGCTACCCGG 
AAAGGAAACA AAATTCGATT TGCAAACCAT TCGGTGAACC CCAACTGTTA TGCCAAAGTG 
GTCATGGTGA ATGGGGATCA TCGAATTGGG ATCTTTGCCA AGAGGGCAAT TCAAGCTGGT 
GAAGAGCTCT TCTTTGATTA CAGGTACAGC CAAGCTGATG CCCTCAAGTA CGTGGGGATC 
GAGCGGGAGA CGGATGTCCT TTAG