EARS2 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	EARS2
Entrez Gene ID	104986579
Full Name	glutamyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010837205.1	XP_010835507.1	probable glutamate--tRNA ligase, mitochondrial isoform X2
XM_010837204.1	XP_010835506.1	probable glutamate--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following EARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi62712	XM_010837204.1 Latest version!	Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBi62713	XM_010837205.1 Latest version!	Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EARS2 ( XM_010837204.1 ) cDNA ORF clone, Bison bison bison -> XP_010835506.1 Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi62712
Clone ID Related Accession (Same CDS sequence)	XM_010837204.1
Accession Version	XM_010837204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	probable glutamate--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494644.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC ACATGGCGGT GCTCCTGAAG AAGTTGCTGC AACCCGGGAG GTCCCCGGCT 
GCCTTGGGCC GCCTGATGGG GCGGCGCGAA GCCAGCCTGG GCACTGATCC CGGGGCTGCG 
GTGCGAGTGC GGTTCGCCCC CAGCCCCACA GGCTTCTTGC ACCTGGGTGG CCTCCGCACT 
GCCTTGTACA ACTACATCTT TGCCAAGAAG CACCAAGGGA GCTTCATCCT GAGGCTGGAG 
GACACAGATC AGACCCGTCT TGTGCCTGGG GCAGCGGAGA ATATTGAGGA CATGCTCGAG 
TGGGCAGGCG TTCCCCCGGA CGAGAGCCCC CGGCGCGGAG GGCCTGCTGG GCCCTACCTT 
CAGTCTCAGC GGCTTGCGCT CTATGCCCAG GCCGCTGAAG CCCTGCTGAA GAGCGGTGCT 
GCGTACCCAT GTTTCTGCTC ACCCCAGCGG CTGGAGCTCC TGAAGAAGGA GGCCCTGAGG 
AACCGCCAGA CGCCCCGGTA TGACAATCGG TGCCGGAGCC TGAGCCAGGC GCAGGTGGCC 
CAGAAGCTGG CCACGGACCC CAAGCCTGCC ATCCGCTTCC GCCTGGAGGG GGAGGCGCCA 
GCCTTCCAGG ACTTGGTATA CGGCTGGAAC CGGCATGAGG TGGCCAGCGT GGAGGGGGAC 
CCAGTCATCC TGAAGAGCGA TGGCTTCCCC ACGTACCACC TGGCCTCCGT GGTGGACGAC 
CACCACATGG GCATCAGCCA CGTGCTGCGG GGCTCCGAGT GGCTGGTCTC CACCTCCAAG 
CACCTGCTCC TCTACCAGGC CCTGGGCTGG CAGCCCCCAC AGTTTGCCCA CCTGCCTCTG 
CTCCTCAACA GGGATGGCAG CAAGCTGTCC AAGAGGCAAG GGGACATCTT CCTGGAGCAT 
TTTGCTGCCA CTGGCTTCCT GCCGGACGCC TTGCTCGACA TCATCACCAA CTGCGGTTCA 
GGGTTTGCAG AGAACCAGAT GGGCCGGACC TTGCCAGAGC TGATCACACA GTTTGACCTG 
AGCCGGGTCA CCTGCCACTC AGCCCTGCTG GACCTGGAGA AGCTTCCGGA ATTCAACAGA 
CTTTTAGAAG GACCTGGTAC GAACTTAACT CAGGACGTGC TGAATAGAGA ACTGAAGAAG 
CTGTCAGATG GTCTGGAAGG CATCAAACAC AGTAACGTGA TGAAGCTCCT CCGAGTGGCC 
CTCAGTGGAC AGCAGCAAGG ACCTCCGGTA GCTGAGATGA TGGTGTCTTT GGGACCAAAG 
GAAGTGCGGG AACGAATACA GAAGGTGCTC TGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010835506.1
CDS	80..1378
Translation	MPPHMAVLLKKLLQPGRSPAALGRLMGRREASLGTDPGAAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKHQGSFILRLEDTDQTRLVPGAAENIEDMLEWAGVPPDESPRRGGPAGPYLQSQRLALYAQAAEALLKSGAAYPCFCSPQRLELLKKEALRNRQTPRYDNRCRSLSQAQVAQKLATDPKPAIRFRLEGEAPAFQDLVYGWNRHEVASVEGDPVILKSDGFPTYHLASVVDDHHMGISHVLRGSEWLVSTSKHLLLYQALGWQPPQFAHLPLLLNRDGSKLSKRQGDIFLEHFAATGFLPDALLDIITNCGSGFAENQMGRTLPELITQFDLSRVTCHSALLDLEKLPEFNRLLEGPGTNLTQDVLNRELKKLSDGLEGIKHSNVMKLLRVALSGQQQGPPVAEMMVSLGPKEVRERIQKVLCS

Target ORF information:

RefSeq Version	XM_010837204.1
Organism	Bison bison bison
Definition	Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010837204.1

ORF Insert Sequence:

ATGCCACCCC ACATGGCGGT GCTCCTGAAG AAGTTGCTGC AACCCGGGAG GTCCCCGGCT 
GCCTTGGGCC GCCTGATGGG GCGGCGCGAA GCCAGCCTGG GCACTGATCC CGGGGCTGCG 
GTGCGAGTGC GGTTCGCCCC CAGCCCCACA GGCTTCTTGC ACCTGGGTGG CCTCCGCACT 
GCCTTGTACA ACTACATCTT TGCCAAGAAG CACCAAGGGA GCTTCATCCT GAGGCTGGAG 
GACACAGATC AGACCCGTCT TGTGCCTGGG GCAGCGGAGA ATATTGAGGA CATGCTCGAG 
TGGGCAGGCG TTCCCCCGGA CGAGAGCCCC CGGCGCGGAG GGCCTGCTGG GCCCTACCTT 
CAGTCTCAGC GGCTTGCGCT CTATGCCCAG GCCGCTGAAG CCCTGCTGAA GAGCGGTGCT 
GCGTACCCAT GTTTCTGCTC ACCCCAGCGG CTGGAGCTCC TGAAGAAGGA GGCCCTGAGG 
AACCGCCAGA CGCCCCGGTA TGACAATCGG TGCCGGAGCC TGAGCCAGGC GCAGGTGGCC 
CAGAAGCTGG CCACGGACCC CAAGCCTGCC ATCCGCTTCC GCCTGGAGGG GGAGGCGCCA 
GCCTTCCAGG ACTTGGTATA CGGCTGGAAC CGGCATGAGG TGGCCAGCGT GGAGGGGGAC 
CCAGTCATCC TGAAGAGCGA TGGCTTCCCC ACGTACCACC TGGCCTCCGT GGTGGACGAC 
CACCACATGG GCATCAGCCA CGTGCTGCGG GGCTCCGAGT GGCTGGTCTC CACCTCCAAG 
CACCTGCTCC TCTACCAGGC CCTGGGCTGG CAGCCCCCAC AGTTTGCCCA CCTGCCTCTG 
CTCCTCAACA GGGATGGCAG CAAGCTGTCC AAGAGGCAAG GGGACATCTT CCTGGAGCAT 
TTTGCTGCCA CTGGCTTCCT GCCGGACGCC TTGCTCGACA TCATCACCAA CTGCGGTTCA 
GGGTTTGCAG AGAACCAGAT GGGCCGGACC TTGCCAGAGC TGATCACACA GTTTGACCTG 
AGCCGGGTCA CCTGCCACTC AGCCCTGCTG GACCTGGAGA AGCTTCCGGA ATTCAACAGA 
CTTTTAGAAG GACCTGGTAC GAACTTAACT CAGGACGTGC TGAATAGAGA ACTGAAGAAG 
CTGTCAGATG GTCTGGAAGG CATCAAACAC AGTAACGTGA TGAAGCTCCT CCGAGTGGCC 
CTCAGTGGAC AGCAGCAAGG ACCTCCGGTA GCTGAGATGA TGGTGTCTTT GGGACCAAAG 
GAAGTGCGGG AACGAATACA GAAGGTGCTC TGCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EARS2 ( XM_010837205.1 ) cDNA ORF clone, Bison bison bison -> XP_010835507.1 Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi62713
Clone ID Related Accession (Same CDS sequence)	XM_010837205.1
Accession Version	XM_010837205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	probable glutamate--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494644.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGAGT GGGCAGGCGT TCCCCCGGAC GAGAGCCCCC GGCGCGGAGG GCCTGCTGGG 
CCCTACCTTC AGTCTCAGCG GCTTGCGCTC TATGCCCAGG CCGCTGAAGC CCTGCTGAAG 
AGCGGTGCTG CGTACCCATG TTTCTGCTCA CCCCAGCGGC TGGAGCTCCT GAAGAAGGAG 
GCCCTGAGGA ACCGCCAGAC GCCCCGGTAT GACAATCGGT GCCGGAGCCT GAGCCAGGCG 
CAGGTGGCCC AGAAGCTGGC CACGGACCCC AAGCCTGCCA TCCGCTTCCG CCTGGAGGGG 
GAGGCGCCAG CCTTCCAGGA CTTGGTATAC GGCTGGAACC GGCATGAGGT GGCCAGCGTG 
GAGGGGGACC CAGTCATCCT GAAGAGCGAT GGCTTCCCCA CGTACCACCT GGCCTCCGTG 
GTGGACGACC ACCACATGGG CATCAGCCAC GTGCTGCGGG GCTCCGAGTG GCTGGTCTCC 
ACCTCCAAGC ACCTGCTCCT CTACCAGGCC CTGGGCTGGC AGCCCCCACA GTTTGCCCAC 
CTGCCTCTGC TCCTCAACAG GGATGGCAGC AAGCTGTCCA AGAGGCAAGG GGACATCTTC 
CTGGAGCATT TTGCTGCCAC TGGCTTCCTG CCGGACGCCT TGCTCGACAT CATCACCAAC 
TGCGGTTCAG GGTTTGCAGA GAACCAGATG GGCCGGACCT TGCCAGAGCT GATCACACAG 
TTTGACCTGA GCCGGGTCAC CTGCCACTCA GCCCTGCTGG ACCTGGAGAA GCTTCCGGAA 
TTCAACAGAC TTTTAGAAGG ACCTGGTACG AACTTAACTC AGGACGTGCT GAATAGAGAA 
CTGAAGAAGC TGTCAGATGG TCTGGAAGGC ATCAAACACA GTAACGTGAT GAAGCTCCTC 
CGAGTGGCCC TCAGTGGACA GCAGCAAGGA CCTCCGGTAG CTGAGATGAT GGTGTCTTTG 
GGACCAAAGG AAGTGCGGGA ACGAATACAG AAGGTGCTCT GCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010835507.1
CDS	239..1246
Translation	MLEWAGVPPDESPRRGGPAGPYLQSQRLALYAQAAEALLKSGAAYPCFCSPQRLELLKKEALRNRQTPRYDNRCRSLSQAQVAQKLATDPKPAIRFRLEGEAPAFQDLVYGWNRHEVASVEGDPVILKSDGFPTYHLASVVDDHHMGISHVLRGSEWLVSTSKHLLLYQALGWQPPQFAHLPLLLNRDGSKLSKRQGDIFLEHFAATGFLPDALLDIITNCGSGFAENQMGRTLPELITQFDLSRVTCHSALLDLEKLPEFNRLLEGPGTNLTQDVLNRELKKLSDGLEGIKHSNVMKLLRVALSGQQQGPPVAEMMVSLGPKEVRERIQKVLCS

Target ORF information:

RefSeq Version	XM_010837205.1
Organism	Bison bison bison
Definition	Bison bison bison glutamyl-tRNA synthetase 2, mitochondrial (EARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010837205.1

ORF Insert Sequence:

ATGCTCGAGT GGGCAGGCGT TCCCCCGGAC GAGAGCCCCC GGCGCGGAGG GCCTGCTGGG 
CCCTACCTTC AGTCTCAGCG GCTTGCGCTC TATGCCCAGG CCGCTGAAGC CCTGCTGAAG 
AGCGGTGCTG CGTACCCATG TTTCTGCTCA CCCCAGCGGC TGGAGCTCCT GAAGAAGGAG 
GCCCTGAGGA ACCGCCAGAC GCCCCGGTAT GACAATCGGT GCCGGAGCCT GAGCCAGGCG 
CAGGTGGCCC AGAAGCTGGC CACGGACCCC AAGCCTGCCA TCCGCTTCCG CCTGGAGGGG 
GAGGCGCCAG CCTTCCAGGA CTTGGTATAC GGCTGGAACC GGCATGAGGT GGCCAGCGTG 
GAGGGGGACC CAGTCATCCT GAAGAGCGAT GGCTTCCCCA CGTACCACCT GGCCTCCGTG 
GTGGACGACC ACCACATGGG CATCAGCCAC GTGCTGCGGG GCTCCGAGTG GCTGGTCTCC 
ACCTCCAAGC ACCTGCTCCT CTACCAGGCC CTGGGCTGGC AGCCCCCACA GTTTGCCCAC 
CTGCCTCTGC TCCTCAACAG GGATGGCAGC AAGCTGTCCA AGAGGCAAGG GGACATCTTC 
CTGGAGCATT TTGCTGCCAC TGGCTTCCTG CCGGACGCCT TGCTCGACAT CATCACCAAC 
TGCGGTTCAG GGTTTGCAGA GAACCAGATG GGCCGGACCT TGCCAGAGCT GATCACACAG 
TTTGACCTGA GCCGGGTCAC CTGCCACTCA GCCCTGCTGG ACCTGGAGAA GCTTCCGGAA 
TTCAACAGAC TTTTAGAAGG ACCTGGTACG AACTTAACTC AGGACGTGCT GAATAGAGAA 
CTGAAGAAGC TGTCAGATGG TCTGGAAGGC ATCAAACACA GTAACGTGAT GAAGCTCCTC 
CGAGTGGCCC TCAGTGGACA GCAGCAAGGA CCTCCGGTAG CTGAGATGAT GGTGTCTTTG 
GGACCAAAGG AAGTGCGGGA ACGAATACAG AAGGTGCTCT GCAGCTAG