MECOM cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	MECOM
Entrez Gene ID	104985176
Full Name	MDS1 and EVI1 complex locus
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010835204.1	XP_010833506.1	MDS1 and EVI1 complex locus protein EVI1 isoform X2
XM_010835206.1	XP_010833508.1	MDS1 and EVI1 complex locus protein EVI1 isoform X4
XM_010835209.1	XP_010833511.1	MDS1 and EVI1 complex locus protein EVI1 isoform X6
XM_010835207.1	XP_010833509.1	MDS1 and EVI1 complex locus protein EVI1 isoform X5
XM_010835203.1	XP_010833505.1	MDS1 and EVI1 complex locus protein EVI1 isoform X1
XM_010835205.1	XP_010833507.1	MDS1 and EVI1 complex locus protein EVI1 isoform X3
XM_010835208.1	XP_010833510.1	MDS1 and EVI1 complex locus protein EVI1 isoform X6
XM_010835210.1	XP_010833512.1	MDS1 and EVI1 complex locus protein EVI1 isoform X6

Pathways from BioSystems
Gene Ontology

The following MECOM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MECOM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBi61224	XM_010835203.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi61225	XM_010835204.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi61226	XM_010835205.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi61227	XM_010835206.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi61228	XM_010835207.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBi61229	XM_010835210.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBi61229	XM_010835209.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBi61229	XM_010835208.1 Latest version!	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MECOM ( XM_010835203.1 ) cDNA ORF clone, Bison bison bison -> XP_010833505.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi61224
Clone ID Related Accession (Same CDS sequence)	XM_010835203.1
Accession Version	XM_010835203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGCA GGTTTTCACG GACCCTAGCA ACCTTCAGCG GCACATCCGC 
TCTCAGCATG TTGGTGCCCG GGCCCACGCG TGCCCCGAGT GTGGCAAAAC GTTTGCCACT 
TCGTCGGGCC TCAAACAACA CAAGCACATC CACAGCAGTG TGAAGCCCTT TATCTGTGAG 
GTCTGCCATA AATCCTATAC TCAGTTTTCA AACCTTTGTC GTCATAAGCG CATGCATGCT 
GATTGCAGAA CCCAAATCAA GTGCAAAGAC TGTGGACAAA TGTTCAGCAC TACGTCTTCC 
TTAAATAAAC ACAGGAGGTT TTGTGAGGGC AAGAACCATT TTGCGGCAGG TGGATTTTTT 
GGCCAAGGCA TTTCACTTCC TGGAACCCCA GCTATGGATA AAACGTCCAT GGTTAATATG 
AGTCATGCCA ACCCGGGCCT TGCTGACTAT TTTGGCGCCA ATAGGCATCC TGCTGGTCTT 
ACCTTTCCAA CAGCTCCTGG ATTTTCTTTT AGCTTCCCTG GTCTGTTTCC TTCCGGCTTG 
TACCACAGGC CTCCTTTGAT ACCTGCTAGT TCTCCTGTTA AAGGACTGTC AAGTACTGAA 
CAGACAAACA AAAGTCAAAG TCCCCTCATG ACACATCCTC AGATACTGCC AGCTACACAG 
GATATTTTGA AGGCACTATC TAAACACCCA CCTGTAGGGG ACAATAAGCC AGTGGAGCTC 
CAGCCCGAGA GGTCCTCTGA AGAGAGGCCC CTTGAGAAAA TCAGTGACCA GTCAGAGAGT 
AGTGACCTTG ATGATGTCAG TACGCCAAGT GGCAGTGACC TGGAAACAAC CTCGGGCTCT 
GATCTGGAAA GTGACATTGA AAGTGATAAA GAGAAATTTA AAGAAAATGG TAAAATGTTC 
AAAGACAAAG TAAGCCCTCT TCAGAATCTG GCTTCAATAC ATAATAAGAA AGAATACAGC 
AATCATTCCA TTTTCTCACC ATCTTTAGAG GAGCAGACTG CGGTGTCAGG AGCTGTGAAT 
GATTCTATAA AGGCTATTGC TTCTATTGCT GAAAAATACT TTGGTTCAAC AGGACTGGTG 
GGGCTGCAAG ACAAAAAAGT TGGAGCTTTA CCTTACCCTT CCATGTTTCC CCTCCCATTT 
TTTCCAGCAT TCTCTCAATC AATGTACCCA TTTCCTGATA GAGACTTGAG ATCGTTACCT 
TTGAAAATGG AGCCCCAATC ACCAAGTGAA GTAAAGAAAC TGCAGAAGGG AAGCTCTGAG 
TCTCCCTTTG ATCTCACCAC TAAACGCAAG GACGAGAAGC CCTTAACCCC AGTACCTGCC 
AAGCCTTCAG CGACCCCGGC TGCAAGCCAA GACCAGCCCC TGGATCTAAG TATGGGCAGT 
AGGAGTAGAG CCAGTGGAAC CAAGCTGACT GAGCCTCGTA AAAACCACGT GTTTGGGGAG 
AAGAAAGGAG GCAACATTGA ACCAAGACCA GCATCCGATG GTTCCTTACA GCATGCTAGA 
CCCACTCCTT TCTTTATGGA CCCTATTTAC AGAGTAGAGA AAAGAAAACT AACTGACCCA 
CTTGAAGCTT TAAAAGAGAA ATACTTGAGG CCTTCTCCAG GATTCTTATT TCACCCACAA 
TTCCAACTGC CTGATCAGAG AACTTGGATG TCGGCTATTG AAAACATGGC AGAGAAGCTG 
GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC AGTGAACTTC TACAGTCTGT TCCCTCCATG 
TTCAACTTCA GAGCTCCTCC CAACGCCCTG CCAGAGAACC TCCTACGGAA AGGAAAGGAG 
CGCTATACCT GCAGATACTG TGGTAAGATT TTTCCAAGAT CTGCGAACCT AACACGGCAC 
TTGAGAACCC ACACAGGAGA GCAGCCTTAT AGATGCAAGT ACTGTGACAG GTCCTTCAGC 
ATATCTTCCA ACCTGCAGCG GCATGTTCGC AACATCCACA ATAAAGAGAA GCCCTTTAAG 
TGTCACTTAT GTGATAGGTG TTTTGGTCAA CAAACCAACT TAGACAGACA CCTCAAGAAG 
CACGAGAATG GGAACATGTC TGGTACGGCA ACATCATCGC CTCATTCTGA ACTGGAAAGC 
ACAGGTGCAA TCCTCGATGA CAAGGAAGAT GCTTACTTCA CAGAAATTCG AAATTTCATC 
GGGAATAGCA ACCACGGCAG CCAGTCCCCC AGGAACGCCG AAGAGAGAAT GAATGGCAGT 
CACTTTAAAG ATGAAAAGGC TTTGGTGACC AATCAAAATT CAGACTTACT GGATGATGAA 
GAAGTAGAAG ATGAGGTGTT GTTAGATGAG GAGGATGAAG ACAATGATAT TACTGGGAAA 
ATGGGAAAGG AACCAGGGAC GAGTAATTTA CATGAAGGAA ACCCCGAGGA TGACTATGAA 
GACACCAGCA CCCTGGAAAT GAGCTGTAAA GCATCCCCAA TGAGGTATAA AGAGGAAGAG 
TATAAAACTG GACTTTCTGC TCTAGATCAT ATAAGGCACT TCACAGATAG CCTCAAAATG 
AGGAAAATGG AAGATAATCA ATATACAGAA GCTGAGGTGT CTTCTTTCAG TGCTTCCCAT 
GTGCCAGAGG AACTTAAACA ACCGTTACAC AGAAAGTCCA AGTCACAGGC ATACGCTATG 
ATGTTGTCAT TGTCCGACAA GGAGTCCCTG CATTCTTCAT CCCACAGTTC TTCCAACGTG 
TGGCATAGTA TGGCCAGGGC CGCAGCAGAA TCCAGTGCCA TCCAGTCCAT AAGCCACGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833505.1
CDS	29..3751
Translation	MRSKGRARKLATNNECVYGSYPEIPLEEMPDADGVAGALSLSIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEAGEKFGPYVGEQRSNLKDPSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835203.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835203.1

ORF Insert Sequence:

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGCA GGTTTTCACG GACCCTAGCA ACCTTCAGCG GCACATCCGC 
TCTCAGCATG TTGGTGCCCG GGCCCACGCG TGCCCCGAGT GTGGCAAAAC GTTTGCCACT 
TCGTCGGGCC TCAAACAACA CAAGCACATC CACAGCAGTG TGAAGCCCTT TATCTGTGAG 
GTCTGCCATA AATCCTATAC TCAGTTTTCA AACCTTTGTC GTCATAAGCG CATGCATGCT 
GATTGCAGAA CCCAAATCAA GTGCAAAGAC TGTGGACAAA TGTTCAGCAC TACGTCTTCC 
TTAAATAAAC ACAGGAGGTT TTGTGAGGGC AAGAACCATT TTGCGGCAGG TGGATTTTTT 
GGCCAAGGCA TTTCACTTCC TGGAACCCCA GCTATGGATA AAACGTCCAT GGTTAATATG 
AGTCATGCCA ACCCGGGCCT TGCTGACTAT TTTGGCGCCA ATAGGCATCC TGCTGGTCTT 
ACCTTTCCAA CAGCTCCTGG ATTTTCTTTT AGCTTCCCTG GTCTGTTTCC TTCCGGCTTG 
TACCACAGGC CTCCTTTGAT ACCTGCTAGT TCTCCTGTTA AAGGACTGTC AAGTACTGAA 
CAGACAAACA AAAGTCAAAG TCCCCTCATG ACACATCCTC AGATACTGCC AGCTACACAG 
GATATTTTGA AGGCACTATC TAAACACCCA CCTGTAGGGG ACAATAAGCC AGTGGAGCTC 
CAGCCCGAGA GGTCCTCTGA AGAGAGGCCC CTTGAGAAAA TCAGTGACCA GTCAGAGAGT 
AGTGACCTTG ATGATGTCAG TACGCCAAGT GGCAGTGACC TGGAAACAAC CTCGGGCTCT 
GATCTGGAAA GTGACATTGA AAGTGATAAA GAGAAATTTA AAGAAAATGG TAAAATGTTC 
AAAGACAAAG TAAGCCCTCT TCAGAATCTG GCTTCAATAC ATAATAAGAA AGAATACAGC 
AATCATTCCA TTTTCTCACC ATCTTTAGAG GAGCAGACTG CGGTGTCAGG AGCTGTGAAT 
GATTCTATAA AGGCTATTGC TTCTATTGCT GAAAAATACT TTGGTTCAAC AGGACTGGTG 
GGGCTGCAAG ACAAAAAAGT TGGAGCTTTA CCTTACCCTT CCATGTTTCC CCTCCCATTT 
TTTCCAGCAT TCTCTCAATC AATGTACCCA TTTCCTGATA GAGACTTGAG ATCGTTACCT 
TTGAAAATGG AGCCCCAATC ACCAAGTGAA GTAAAGAAAC TGCAGAAGGG AAGCTCTGAG 
TCTCCCTTTG ATCTCACCAC TAAACGCAAG GACGAGAAGC CCTTAACCCC AGTACCTGCC 
AAGCCTTCAG CGACCCCGGC TGCAAGCCAA GACCAGCCCC TGGATCTAAG TATGGGCAGT 
AGGAGTAGAG CCAGTGGAAC CAAGCTGACT GAGCCTCGTA AAAACCACGT GTTTGGGGAG 
AAGAAAGGAG GCAACATTGA ACCAAGACCA GCATCCGATG GTTCCTTACA GCATGCTAGA 
CCCACTCCTT TCTTTATGGA CCCTATTTAC AGAGTAGAGA AAAGAAAACT AACTGACCCA 
CTTGAAGCTT TAAAAGAGAA ATACTTGAGG CCTTCTCCAG GATTCTTATT TCACCCACAA 
TTCCAACTGC CTGATCAGAG AACTTGGATG TCGGCTATTG AAAACATGGC AGAGAAGCTG 
GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC AGTGAACTTC TACAGTCTGT TCCCTCCATG 
TTCAACTTCA GAGCTCCTCC CAACGCCCTG CCAGAGAACC TCCTACGGAA AGGAAAGGAG 
CGCTATACCT GCAGATACTG TGGTAAGATT TTTCCAAGAT CTGCGAACCT AACACGGCAC 
TTGAGAACCC ACACAGGAGA GCAGCCTTAT AGATGCAAGT ACTGTGACAG GTCCTTCAGC 
ATATCTTCCA ACCTGCAGCG GCATGTTCGC AACATCCACA ATAAAGAGAA GCCCTTTAAG 
TGTCACTTAT GTGATAGGTG TTTTGGTCAA CAAACCAACT TAGACAGACA CCTCAAGAAG 
CACGAGAATG GGAACATGTC TGGTACGGCA ACATCATCGC CTCATTCTGA ACTGGAAAGC 
ACAGGTGCAA TCCTCGATGA CAAGGAAGAT GCTTACTTCA CAGAAATTCG AAATTTCATC 
GGGAATAGCA ACCACGGCAG CCAGTCCCCC AGGAACGCCG AAGAGAGAAT GAATGGCAGT 
CACTTTAAAG ATGAAAAGGC TTTGGTGACC AATCAAAATT CAGACTTACT GGATGATGAA 
GAAGTAGAAG ATGAGGTGTT GTTAGATGAG GAGGATGAAG ACAATGATAT TACTGGGAAA 
ATGGGAAAGG AACCAGGGAC GAGTAATTTA CATGAAGGAA ACCCCGAGGA TGACTATGAA 
GACACCAGCA CCCTGGAAAT GAGCTGTAAA GCATCCCCAA TGAGGTATAA AGAGGAAGAG 
TATAAAACTG GACTTTCTGC TCTAGATCAT ATAAGGCACT TCACAGATAG CCTCAAAATG 
AGGAAAATGG AAGATAATCA ATATACAGAA GCTGAGGTGT CTTCTTTCAG TGCTTCCCAT 
GTGCCAGAGG AACTTAAACA ACCGTTACAC AGAAAGTCCA AGTCACAGGC ATACGCTATG 
ATGTTGTCAT TGTCCGACAA GGAGTCCCTG CATTCTTCAT CCCACAGTTC TTCCAACGTG 
TGGCATAGTA TGGCCAGGGC CGCAGCAGAA TCCAGTGCCA TCCAGTCCAT AAGCCACGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835204.1 ) cDNA ORF clone, Bison bison bison -> XP_010833506.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi61225
Clone ID Related Accession (Same CDS sequence)	XM_010835204.1
Accession Version	XM_010835204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGGT TTTCACGGAC CCTAGCAACC TTCAGCGGCA CATCCGCTCT 
CAGCATGTTG GTGCCCGGGC CCACGCGTGC CCCGAGTGTG GCAAAACGTT TGCCACTTCG 
TCGGGCCTCA AACAACACAA GCACATCCAC AGCAGTGTGA AGCCCTTTAT CTGTGAGGTC 
TGCCATAAAT CCTATACTCA GTTTTCAAAC CTTTGTCGTC ATAAGCGCAT GCATGCTGAT 
TGCAGAACCC AAATCAAGTG CAAAGACTGT GGACAAATGT TCAGCACTAC GTCTTCCTTA 
AATAAACACA GGAGGTTTTG TGAGGGCAAG AACCATTTTG CGGCAGGTGG ATTTTTTGGC 
CAAGGCATTT CACTTCCTGG AACCCCAGCT ATGGATAAAA CGTCCATGGT TAATATGAGT 
CATGCCAACC CGGGCCTTGC TGACTATTTT GGCGCCAATA GGCATCCTGC TGGTCTTACC 
TTTCCAACAG CTCCTGGATT TTCTTTTAGC TTCCCTGGTC TGTTTCCTTC CGGCTTGTAC 
CACAGGCCTC CTTTGATACC TGCTAGTTCT CCTGTTAAAG GACTGTCAAG TACTGAACAG 
ACAAACAAAA GTCAAAGTCC CCTCATGACA CATCCTCAGA TACTGCCAGC TACACAGGAT 
ATTTTGAAGG CACTATCTAA ACACCCACCT GTAGGGGACA ATAAGCCAGT GGAGCTCCAG 
CCCGAGAGGT CCTCTGAAGA GAGGCCCCTT GAGAAAATCA GTGACCAGTC AGAGAGTAGT 
GACCTTGATG ATGTCAGTAC GCCAAGTGGC AGTGACCTGG AAACAACCTC GGGCTCTGAT 
CTGGAAAGTG ACATTGAAAG TGATAAAGAG AAATTTAAAG AAAATGGTAA AATGTTCAAA 
GACAAAGTAA GCCCTCTTCA GAATCTGGCT TCAATACATA ATAAGAAAGA ATACAGCAAT 
CATTCCATTT TCTCACCATC TTTAGAGGAG CAGACTGCGG TGTCAGGAGC TGTGAATGAT 
TCTATAAAGG CTATTGCTTC TATTGCTGAA AAATACTTTG GTTCAACAGG ACTGGTGGGG 
CTGCAAGACA AAAAAGTTGG AGCTTTACCT TACCCTTCCA TGTTTCCCCT CCCATTTTTT 
CCAGCATTCT CTCAATCAAT GTACCCATTT CCTGATAGAG ACTTGAGATC GTTACCTTTG 
AAAATGGAGC CCCAATCACC AAGTGAAGTA AAGAAACTGC AGAAGGGAAG CTCTGAGTCT 
CCCTTTGATC TCACCACTAA ACGCAAGGAC GAGAAGCCCT TAACCCCAGT ACCTGCCAAG 
CCTTCAGCGA CCCCGGCTGC AAGCCAAGAC CAGCCCCTGG ATCTAAGTAT GGGCAGTAGG 
AGTAGAGCCA GTGGAACCAA GCTGACTGAG CCTCGTAAAA ACCACGTGTT TGGGGAGAAG 
AAAGGAGGCA ACATTGAACC AAGACCAGCA TCCGATGGTT CCTTACAGCA TGCTAGACCC 
ACTCCTTTCT TTATGGACCC TATTTACAGA GTAGAGAAAA GAAAACTAAC TGACCCACTT 
GAAGCTTTAA AAGAGAAATA CTTGAGGCCT TCTCCAGGAT TCTTATTTCA CCCACAATTC 
CAACTGCCTG ATCAGAGAAC TTGGATGTCG GCTATTGAAA ACATGGCAGA GAAGCTGGAG 
AGCTTCAGTG CCCTGAAACC AGAGGCCAGT GAACTTCTAC AGTCTGTTCC CTCCATGTTC 
AACTTCAGAG CTCCTCCCAA CGCCCTGCCA GAGAACCTCC TACGGAAAGG AAAGGAGCGC 
TATACCTGCA GATACTGTGG TAAGATTTTT CCAAGATCTG CGAACCTAAC ACGGCACTTG 
AGAACCCACA CAGGAGAGCA GCCTTATAGA TGCAAGTACT GTGACAGGTC CTTCAGCATA 
TCTTCCAACC TGCAGCGGCA TGTTCGCAAC ATCCACAATA AAGAGAAGCC CTTTAAGTGT 
CACTTATGTG ATAGGTGTTT TGGTCAACAA ACCAACTTAG ACAGACACCT CAAGAAGCAC 
GAGAATGGGA ACATGTCTGG TACGGCAACA TCATCGCCTC ATTCTGAACT GGAAAGCACA 
GGTGCAATCC TCGATGACAA GGAAGATGCT TACTTCACAG AAATTCGAAA TTTCATCGGG 
AATAGCAACC ACGGCAGCCA GTCCCCCAGG AACGCCGAAG AGAGAATGAA TGGCAGTCAC 
TTTAAAGATG AAAAGGCTTT GGTGACCAAT CAAAATTCAG ACTTACTGGA TGATGAAGAA 
GTAGAAGATG AGGTGTTGTT AGATGAGGAG GATGAAGACA ATGATATTAC TGGGAAAATG 
GGAAAGGAAC CAGGGACGAG TAATTTACAT GAAGGAAACC CCGAGGATGA CTATGAAGAC 
ACCAGCACCC TGGAAATGAG CTGTAAAGCA TCCCCAATGA GGTATAAAGA GGAAGAGTAT 
AAAACTGGAC TTTCTGCTCT AGATCATATA AGGCACTTCA CAGATAGCCT CAAAATGAGG 
AAAATGGAAG ATAATCAATA TACAGAAGCT GAGGTGTCTT CTTTCAGTGC TTCCCATGTG 
CCAGAGGAAC TTAAACAACC GTTACACAGA AAGTCCAAGT CACAGGCATA CGCTATGATG 
TTGTCATTGT CCGACAAGGA GTCCCTGCAT TCTTCATCCC ACAGTTCTTC CAACGTGTGG 
CATAGTATGG CCAGGGCCGC AGCAGAATCC AGTGCCATCC AGTCCATAAG CCACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833506.1
CDS	29..3748
Translation	MRSKGRARKLATNNECVYGSYPEIPLEEMPDADGVAGALSLSIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEAGEKFGPYVGEQRSNLKDPSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835204.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835204.1

ORF Insert Sequence:

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGGT TTTCACGGAC CCTAGCAACC TTCAGCGGCA CATCCGCTCT 
CAGCATGTTG GTGCCCGGGC CCACGCGTGC CCCGAGTGTG GCAAAACGTT TGCCACTTCG 
TCGGGCCTCA AACAACACAA GCACATCCAC AGCAGTGTGA AGCCCTTTAT CTGTGAGGTC 
TGCCATAAAT CCTATACTCA GTTTTCAAAC CTTTGTCGTC ATAAGCGCAT GCATGCTGAT 
TGCAGAACCC AAATCAAGTG CAAAGACTGT GGACAAATGT TCAGCACTAC GTCTTCCTTA 
AATAAACACA GGAGGTTTTG TGAGGGCAAG AACCATTTTG CGGCAGGTGG ATTTTTTGGC 
CAAGGCATTT CACTTCCTGG AACCCCAGCT ATGGATAAAA CGTCCATGGT TAATATGAGT 
CATGCCAACC CGGGCCTTGC TGACTATTTT GGCGCCAATA GGCATCCTGC TGGTCTTACC 
TTTCCAACAG CTCCTGGATT TTCTTTTAGC TTCCCTGGTC TGTTTCCTTC CGGCTTGTAC 
CACAGGCCTC CTTTGATACC TGCTAGTTCT CCTGTTAAAG GACTGTCAAG TACTGAACAG 
ACAAACAAAA GTCAAAGTCC CCTCATGACA CATCCTCAGA TACTGCCAGC TACACAGGAT 
ATTTTGAAGG CACTATCTAA ACACCCACCT GTAGGGGACA ATAAGCCAGT GGAGCTCCAG 
CCCGAGAGGT CCTCTGAAGA GAGGCCCCTT GAGAAAATCA GTGACCAGTC AGAGAGTAGT 
GACCTTGATG ATGTCAGTAC GCCAAGTGGC AGTGACCTGG AAACAACCTC GGGCTCTGAT 
CTGGAAAGTG ACATTGAAAG TGATAAAGAG AAATTTAAAG AAAATGGTAA AATGTTCAAA 
GACAAAGTAA GCCCTCTTCA GAATCTGGCT TCAATACATA ATAAGAAAGA ATACAGCAAT 
CATTCCATTT TCTCACCATC TTTAGAGGAG CAGACTGCGG TGTCAGGAGC TGTGAATGAT 
TCTATAAAGG CTATTGCTTC TATTGCTGAA AAATACTTTG GTTCAACAGG ACTGGTGGGG 
CTGCAAGACA AAAAAGTTGG AGCTTTACCT TACCCTTCCA TGTTTCCCCT CCCATTTTTT 
CCAGCATTCT CTCAATCAAT GTACCCATTT CCTGATAGAG ACTTGAGATC GTTACCTTTG 
AAAATGGAGC CCCAATCACC AAGTGAAGTA AAGAAACTGC AGAAGGGAAG CTCTGAGTCT 
CCCTTTGATC TCACCACTAA ACGCAAGGAC GAGAAGCCCT TAACCCCAGT ACCTGCCAAG 
CCTTCAGCGA CCCCGGCTGC AAGCCAAGAC CAGCCCCTGG ATCTAAGTAT GGGCAGTAGG 
AGTAGAGCCA GTGGAACCAA GCTGACTGAG CCTCGTAAAA ACCACGTGTT TGGGGAGAAG 
AAAGGAGGCA ACATTGAACC AAGACCAGCA TCCGATGGTT CCTTACAGCA TGCTAGACCC 
ACTCCTTTCT TTATGGACCC TATTTACAGA GTAGAGAAAA GAAAACTAAC TGACCCACTT 
GAAGCTTTAA AAGAGAAATA CTTGAGGCCT TCTCCAGGAT TCTTATTTCA CCCACAATTC 
CAACTGCCTG ATCAGAGAAC TTGGATGTCG GCTATTGAAA ACATGGCAGA GAAGCTGGAG 
AGCTTCAGTG CCCTGAAACC AGAGGCCAGT GAACTTCTAC AGTCTGTTCC CTCCATGTTC 
AACTTCAGAG CTCCTCCCAA CGCCCTGCCA GAGAACCTCC TACGGAAAGG AAAGGAGCGC 
TATACCTGCA GATACTGTGG TAAGATTTTT CCAAGATCTG CGAACCTAAC ACGGCACTTG 
AGAACCCACA CAGGAGAGCA GCCTTATAGA TGCAAGTACT GTGACAGGTC CTTCAGCATA 
TCTTCCAACC TGCAGCGGCA TGTTCGCAAC ATCCACAATA AAGAGAAGCC CTTTAAGTGT 
CACTTATGTG ATAGGTGTTT TGGTCAACAA ACCAACTTAG ACAGACACCT CAAGAAGCAC 
GAGAATGGGA ACATGTCTGG TACGGCAACA TCATCGCCTC ATTCTGAACT GGAAAGCACA 
GGTGCAATCC TCGATGACAA GGAAGATGCT TACTTCACAG AAATTCGAAA TTTCATCGGG 
AATAGCAACC ACGGCAGCCA GTCCCCCAGG AACGCCGAAG AGAGAATGAA TGGCAGTCAC 
TTTAAAGATG AAAAGGCTTT GGTGACCAAT CAAAATTCAG ACTTACTGGA TGATGAAGAA 
GTAGAAGATG AGGTGTTGTT AGATGAGGAG GATGAAGACA ATGATATTAC TGGGAAAATG 
GGAAAGGAAC CAGGGACGAG TAATTTACAT GAAGGAAACC CCGAGGATGA CTATGAAGAC 
ACCAGCACCC TGGAAATGAG CTGTAAAGCA TCCCCAATGA GGTATAAAGA GGAAGAGTAT 
AAAACTGGAC TTTCTGCTCT AGATCATATA AGGCACTTCA CAGATAGCCT CAAAATGAGG 
AAAATGGAAG ATAATCAATA TACAGAAGCT GAGGTGTCTT CTTTCAGTGC TTCCCATGTG 
CCAGAGGAAC TTAAACAACC GTTACACAGA AAGTCCAAGT CACAGGCATA CGCTATGATG 
TTGTCATTGT CCGACAAGGA GTCCCTGCAT TCTTCATCCC ACAGTTCTTC CAACGTGTGG 
CATAGTATGG CCAGGGCCGC AGCAGAATCC AGTGCCATCC AGTCCATAAG CCACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835205.1 ) cDNA ORF clone, Bison bison bison -> XP_010833507.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X3, mRNA.

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CloneID	OBi61226
Clone ID Related Accession (Same CDS sequence)	XM_010835205.1
Accession Version	XM_010835205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGGT TTTCACGGAC CCTAGCAACC TTCAGCGGCA CATCCGCTCT 
CAGCATGTTG GTGCCCGGGC CCACGCGTGC CCCGAGTGTG GCAAAACGTT TGCCACTTCG 
TCGGGCCTCA AACAACACAA GCACATCCAC AGCAGTGTGA AGCCCTTTAT CTGTGAGGTC 
TGCCATAAAT CCTATACTCA GTTTTCAAAC CTTTGTCGTC ATAAGCGCAT GCATGCTGAT 
TGCAGAACCC AAATCAAGTG CAAAGACTGT GGACAAATGT TCAGCACTAC GTCTTCCTTA 
AATAAACACA GGAGGTTTTG TGAGGGCAAG AACCATTTTG CGGCAGGTGG ATTTTTTGGC 
CAAGGCATTT CACTTCCTGG AACCCCAGCT ATGGATAAAA CGTCCATGGT TAATATGAGT 
CATGCCAACC CGGGCCTTGC TGACTATTTT GGCGCCAATA GGCATCCTGC TGGTCTTACC 
TTTCCAACAG CTCCTGGATT TTCTTTTAGC TTCCCTGGTC TGTTTCCTTC CGGCTTGTAC 
CACAGGCCTC CTTTGATACC TGCTAGTTCT CCTGTTAAAG GACTGTCAAG TACTGAACAG 
ACAAACAAAA GTCAAAGTCC CCTCATGACA CATCCTCAGA TACTGCCAGC TACACAGGAT 
ATTTTGAAGG CACTATCTAA ACACCCACCT GTAGGGGACA ATAAGCCAGT GGAGCTCCAG 
CCCGAGAGGT CCTCTGAAGA GAGGCCCCTT GAGAAAATCA GTGACCAGTC AGAGAGTAGT 
GACCTTGATG ATGTCAGTAC GCCAAGTGGC AGTGACCTGG AAACAACCTC GGGCTCTGAT 
CTGGAAAGTG ACATTGAAAG TGATAAAGAG AAATTTAAAG AAAATGGTAA AATGTTCAAA 
GACAAAGTAA GCCCTCTTCA GAATCTGGCT TCAATACATA ATAAGAAAGA ATACAGCAAT 
CATTCCATTT TCTCACCATC TTTAGAGGAG CAGACTGCGG TGTCAGGAGC TGTGAATGAT 
TCTATAAAGG CTATTGCTTC TATTGCTGAA AAATACTTTG GTTCAACAGG ACTGGTGGGG 
CTGCAAGACA AAAAAGTTGG AGCTTTACCT TACCCTTCCA TGTTTCCCCT CCCATTTTTT 
CCAGCATTCT CTCAATCAAT GTACCCATTT CCTGATAGAG ACTTGAGATC GTTACCTTTG 
AAAATGGAGC CCCAATCACC AAGTGAAGTA AAGAAACTGC AGAAGGGAAG CTCTGAGTCT 
CCCTTTGATC TCACCACTAA ACGCAAGGAC GAGAAGCCCT TAACCCCAGT ACCTGCCAAG 
CCTTCAGCGA CCCCGGCTGC AAGCCAAGAC CAGCCCCTGG ATCTAAGTAT GGGCAGTAGG 
AGTAGAGCCA GTGGAACCAA GCTGACTGAG CCTCGTAAAA ACCACGTGTT TGGGGAGAAG 
AAAGGAGGCA ACATTGAACC AAGACCAGCA TCCGATGGTT CCTTACAGCA TGCTAGACCC 
ACTCCTTTCT TTATGGACCC TATTTACAGA GTAGAGAAAA GAAAACTAAC TGACCCACTT 
GAAGCTTTAA AAGAGAAATA CTTGAGGCCT TCTCCAGGAT TCTTATTTCA CCCACAAATG 
TCGGCTATTG AAAACATGGC AGAGAAGCTG GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC 
AGTGAACTTC TACAGTCTGT TCCCTCCATG TTCAACTTCA GAGCTCCTCC CAACGCCCTG 
CCAGAGAACC TCCTACGGAA AGGAAAGGAG CGCTATACCT GCAGATACTG TGGTAAGATT 
TTTCCAAGAT CTGCGAACCT AACACGGCAC TTGAGAACCC ACACAGGAGA GCAGCCTTAT 
AGATGCAAGT ACTGTGACAG GTCCTTCAGC ATATCTTCCA ACCTGCAGCG GCATGTTCGC 
AACATCCACA ATAAAGAGAA GCCCTTTAAG TGTCACTTAT GTGATAGGTG TTTTGGTCAA 
CAAACCAACT TAGACAGACA CCTCAAGAAG CACGAGAATG GGAACATGTC TGGTACGGCA 
ACATCATCGC CTCATTCTGA ACTGGAAAGC ACAGGTGCAA TCCTCGATGA CAAGGAAGAT 
GCTTACTTCA CAGAAATTCG AAATTTCATC GGGAATAGCA ACCACGGCAG CCAGTCCCCC 
AGGAACGCCG AAGAGAGAAT GAATGGCAGT CACTTTAAAG ATGAAAAGGC TTTGGTGACC 
AATCAAAATT CAGACTTACT GGATGATGAA GAAGTAGAAG ATGAGGTGTT GTTAGATGAG 
GAGGATGAAG ACAATGATAT TACTGGGAAA ATGGGAAAGG AACCAGGGAC GAGTAATTTA 
CATGAAGGAA ACCCCGAGGA TGACTATGAA GACACCAGCA CCCTGGAAAT GAGCTGTAAA 
GCATCCCCAA TGAGGTATAA AGAGGAAGAG TATAAAACTG GACTTTCTGC TCTAGATCAT 
ATAAGGCACT TCACAGATAG CCTCAAAATG AGGAAAATGG AAGATAATCA ATATACAGAA 
GCTGAGGTGT CTTCTTTCAG TGCTTCCCAT GTGCCAGAGG AACTTAAACA ACCGTTACAC 
AGAAAGTCCA AGTCACAGGC ATACGCTATG ATGTTGTCAT TGTCCGACAA GGAGTCCCTG 
CATTCTTCAT CCCACAGTTC TTCCAACGTG TGGCATAGTA TGGCCAGGGC CGCAGCAGAA 
TCCAGTGCCA TCCAGTCCAT AAGCCACGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833507.1
CDS	29..3721
Translation	MRSKGRARKLATNNECVYGSYPEIPLEEMPDADGVAGALSLSIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEAGEKFGPYVGEQRSNLKDPSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835205.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835205.1

ORF Insert Sequence:

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGGT TTTCACGGAC CCTAGCAACC TTCAGCGGCA CATCCGCTCT 
CAGCATGTTG GTGCCCGGGC CCACGCGTGC CCCGAGTGTG GCAAAACGTT TGCCACTTCG 
TCGGGCCTCA AACAACACAA GCACATCCAC AGCAGTGTGA AGCCCTTTAT CTGTGAGGTC 
TGCCATAAAT CCTATACTCA GTTTTCAAAC CTTTGTCGTC ATAAGCGCAT GCATGCTGAT 
TGCAGAACCC AAATCAAGTG CAAAGACTGT GGACAAATGT TCAGCACTAC GTCTTCCTTA 
AATAAACACA GGAGGTTTTG TGAGGGCAAG AACCATTTTG CGGCAGGTGG ATTTTTTGGC 
CAAGGCATTT CACTTCCTGG AACCCCAGCT ATGGATAAAA CGTCCATGGT TAATATGAGT 
CATGCCAACC CGGGCCTTGC TGACTATTTT GGCGCCAATA GGCATCCTGC TGGTCTTACC 
TTTCCAACAG CTCCTGGATT TTCTTTTAGC TTCCCTGGTC TGTTTCCTTC CGGCTTGTAC 
CACAGGCCTC CTTTGATACC TGCTAGTTCT CCTGTTAAAG GACTGTCAAG TACTGAACAG 
ACAAACAAAA GTCAAAGTCC CCTCATGACA CATCCTCAGA TACTGCCAGC TACACAGGAT 
ATTTTGAAGG CACTATCTAA ACACCCACCT GTAGGGGACA ATAAGCCAGT GGAGCTCCAG 
CCCGAGAGGT CCTCTGAAGA GAGGCCCCTT GAGAAAATCA GTGACCAGTC AGAGAGTAGT 
GACCTTGATG ATGTCAGTAC GCCAAGTGGC AGTGACCTGG AAACAACCTC GGGCTCTGAT 
CTGGAAAGTG ACATTGAAAG TGATAAAGAG AAATTTAAAG AAAATGGTAA AATGTTCAAA 
GACAAAGTAA GCCCTCTTCA GAATCTGGCT TCAATACATA ATAAGAAAGA ATACAGCAAT 
CATTCCATTT TCTCACCATC TTTAGAGGAG CAGACTGCGG TGTCAGGAGC TGTGAATGAT 
TCTATAAAGG CTATTGCTTC TATTGCTGAA AAATACTTTG GTTCAACAGG ACTGGTGGGG 
CTGCAAGACA AAAAAGTTGG AGCTTTACCT TACCCTTCCA TGTTTCCCCT CCCATTTTTT 
CCAGCATTCT CTCAATCAAT GTACCCATTT CCTGATAGAG ACTTGAGATC GTTACCTTTG 
AAAATGGAGC CCCAATCACC AAGTGAAGTA AAGAAACTGC AGAAGGGAAG CTCTGAGTCT 
CCCTTTGATC TCACCACTAA ACGCAAGGAC GAGAAGCCCT TAACCCCAGT ACCTGCCAAG 
CCTTCAGCGA CCCCGGCTGC AAGCCAAGAC CAGCCCCTGG ATCTAAGTAT GGGCAGTAGG 
AGTAGAGCCA GTGGAACCAA GCTGACTGAG CCTCGTAAAA ACCACGTGTT TGGGGAGAAG 
AAAGGAGGCA ACATTGAACC AAGACCAGCA TCCGATGGTT CCTTACAGCA TGCTAGACCC 
ACTCCTTTCT TTATGGACCC TATTTACAGA GTAGAGAAAA GAAAACTAAC TGACCCACTT 
GAAGCTTTAA AAGAGAAATA CTTGAGGCCT TCTCCAGGAT TCTTATTTCA CCCACAAATG 
TCGGCTATTG AAAACATGGC AGAGAAGCTG GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC 
AGTGAACTTC TACAGTCTGT TCCCTCCATG TTCAACTTCA GAGCTCCTCC CAACGCCCTG 
CCAGAGAACC TCCTACGGAA AGGAAAGGAG CGCTATACCT GCAGATACTG TGGTAAGATT 
TTTCCAAGAT CTGCGAACCT AACACGGCAC TTGAGAACCC ACACAGGAGA GCAGCCTTAT 
AGATGCAAGT ACTGTGACAG GTCCTTCAGC ATATCTTCCA ACCTGCAGCG GCATGTTCGC 
AACATCCACA ATAAAGAGAA GCCCTTTAAG TGTCACTTAT GTGATAGGTG TTTTGGTCAA 
CAAACCAACT TAGACAGACA CCTCAAGAAG CACGAGAATG GGAACATGTC TGGTACGGCA 
ACATCATCGC CTCATTCTGA ACTGGAAAGC ACAGGTGCAA TCCTCGATGA CAAGGAAGAT 
GCTTACTTCA CAGAAATTCG AAATTTCATC GGGAATAGCA ACCACGGCAG CCAGTCCCCC 
AGGAACGCCG AAGAGAGAAT GAATGGCAGT CACTTTAAAG ATGAAAAGGC TTTGGTGACC 
AATCAAAATT CAGACTTACT GGATGATGAA GAAGTAGAAG ATGAGGTGTT GTTAGATGAG 
GAGGATGAAG ACAATGATAT TACTGGGAAA ATGGGAAAGG AACCAGGGAC GAGTAATTTA 
CATGAAGGAA ACCCCGAGGA TGACTATGAA GACACCAGCA CCCTGGAAAT GAGCTGTAAA 
GCATCCCCAA TGAGGTATAA AGAGGAAGAG TATAAAACTG GACTTTCTGC TCTAGATCAT 
ATAAGGCACT TCACAGATAG CCTCAAAATG AGGAAAATGG AAGATAATCA ATATACAGAA 
GCTGAGGTGT CTTCTTTCAG TGCTTCCCAT GTGCCAGAGG AACTTAAACA ACCGTTACAC 
AGAAAGTCCA AGTCACAGGC ATACGCTATG ATGTTGTCAT TGTCCGACAA GGAGTCCCTG 
CATTCTTCAT CCCACAGTTC TTCCAACGTG TGGCATAGTA TGGCCAGGGC CGCAGCAGAA 
TCCAGTGCCA TCCAGTCCAT AAGCCACGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835206.1 ) cDNA ORF clone, Bison bison bison -> XP_010833508.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBi61227
Clone ID Related Accession (Same CDS sequence)	XM_010835206.1
Accession Version	XM_010835206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGCA GGTTTTCACG GACCCTAGCA ACCTTCAGCG GCACATCCGC 
TCTCAGCATG TTGGTGCCCG GGCCCACGCG TGCCCCGAGT GTGGCAAAAC GTTTGCCACT 
TCGTCGGGCC TCAAACAACA CAAGCACATC CACAGCAGTG TGAAGCCCTT TATCTGTGAG 
GTCTGCCATA AATCCTATAC TCAGTTTTCA AACCTTTGTC GTCATAAGCG CATGCATGCT 
GATTGCAGAA CCCAAATCAA GTGCAAAGAC TGTGGACAAA TGTTCAGCAC TACGTCTTCC 
TTAAATAAAC ACAGGAGGTT TTGTGAGGGC AAGAACCATT TTGCGGCAGG TGGATTTTTT 
GGCCAAGGCA TTTCACTTCC TGGAACCCCA GCTATGGATA AAACGTCCAT GGTTAATATG 
AGTCATGCCA ACCCGGGCCT TGCTGACTAT TTTGGCGCCA ATAGGCATCC TGCTGGTCTT 
ACCTTTCCAA CAGCTCCTGG ATTTTCTTTT AGCTTCCCTG GTCTGTTTCC TTCCGGCTTG 
TACCACAGGC CTCCTTTGAT ACCTGCTAGT TCTCCTGTTA AAGGACTGTC AAGTACTGAA 
CAGACAAACA AAAGTCAAAG TCCCCTCATG ACACATCCTC AGATACTGCC AGCTACACAG 
GATATTTTGA AGGCACTATC TAAACACCCA CCTGTAGGGG ACAATAAGCC AGTGGAGCTC 
CAGCCCGAGA GGTCCTCTGA AGAGAGGCCC CTTGAGAAAA TCAGTGACCA GTCAGAGAGT 
AGTGACCTTG ATGATGTCAG TACGCCAAGT GGCAGTGACC TGGAAACAAC CTCGGGCTCT 
GATCTGGAAA GTGACATTGA AAGTGATAAA GAGAAATTTA AAGAAAATGG TAAAATGTTC 
AAAGACAAAG TAAGCCCTCT TCAGAATCTG GCTTCAATAC ATAATAAGAA AGAATACAGC 
AATCATTCCA TTTTCTCACC ATCTTTAGAG GAGCAGACTG CGGTGTCAGG AGCTGTGAAT 
GATTCTATAA AGGCTATTGC TTCTATTGCT GAAAAATACT TTGGTTCAAC AGGACTGGTG 
GGGCTGCAAG ACAAAAAAGT TGGAGCTTTA CCTTACCCTT CCATGTTTCC CCTCCCATTT 
TTTCCAGCAT TCTCTCAATC AATGTACCCA TTTCCTGATA GAGACTTGAG ATCGTTACCT 
TTGAAAATGG AGCCCCAATC ACCAAGTGAA GTAAAGAAAC TGCAGAAGGG AAGCTCTGAG 
TCTCCCTTTG ATCTCACCAC TAAACGCAAG GACGAGAAGC CCTTAACCCC AGTACCTGCC 
AAGCCTTCAG CGACCCCGGC TGCAAGCCAA GACCAGCCCC TGGATCTAAG TATGGGCAGT 
AGGAGTAGAG CCAGTGGAAC CAAGCTGACT GAGCCTCGTA AAAACCACGT GTTTGGGGAG 
AAGAAAGGAG GCAACATTGA ACCAAGACCA GCATCCGATG GTTCCTTACA GCATGCTAGA 
CCCACTCCTT TCTTTATGGA CCCTATTTAC AGAGTAGAGA AAAGAAAACT AACTGACCCA 
CTTGAAGCTT TAAAAGAGAA ATACTTGAGG CCTTCTCCAG GATTCTTATT TCACCCACAA 
TTCCAACTGC CTGATCAGAG AACTTGGATG TCGGCTATTG AAAACATGGC AGAGAAGCTG 
GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC AGTGAACTTC TACAGTCTGT TCCCTCCATG 
TTCAACTTCA GAGCTCCTCC CAACGCCCTG CCAGAGAACC TCCTACGGAA AGGAAAGGAG 
CGCTATACCT GCAGATGCAA GTACTGTGAC AGGTCCTTCA GCATATCTTC CAACCTGCAG 
CGGCATGTTC GCAACATCCA CAATAAAGAG AAGCCCTTTA AGTGTCACTT ATGTGATAGG 
TGTTTTGGTC AACAAACCAA CTTAGACAGA CACCTCAAGA AGCACGAGAA TGGGAACATG 
TCTGGTACGG CAACATCATC GCCTCATTCT GAACTGGAAA GCACAGGTGC AATCCTCGAT 
GACAAGGAAG ATGCTTACTT CACAGAAATT CGAAATTTCA TCGGGAATAG CAACCACGGC 
AGCCAGTCCC CCAGGAACGC CGAAGAGAGA ATGAATGGCA GTCACTTTAA AGATGAAAAG 
GCTTTGGTGA CCAATCAAAA TTCAGACTTA CTGGATGATG AAGAAGTAGA AGATGAGGTG 
TTGTTAGATG AGGAGGATGA AGACAATGAT ATTACTGGGA AAATGGGAAA GGAACCAGGG 
ACGAGTAATT TACATGAAGG AAACCCCGAG GATGACTATG AAGACACCAG CACCCTGGAA 
ATGAGCTGTA AAGCATCCCC AATGAGGTAT AAAGAGGAAG AGTATAAAAC TGGACTTTCT 
GCTCTAGATC ATATAAGGCA CTTCACAGAT AGCCTCAAAA TGAGGAAAAT GGAAGATAAT 
CAATATACAG AAGCTGAGGT GTCTTCTTTC AGTGCTTCCC ATGTGCCAGA GGAACTTAAA 
CAACCGTTAC ACAGAAAGTC CAAGTCACAG GCATACGCTA TGATGTTGTC ATTGTCCGAC 
AAGGAGTCCC TGCATTCTTC ATCCCACAGT TCTTCCAACG TGTGGCATAG TATGGCCAGG 
GCCGCAGCAG AATCCAGTGC CATCCAGTCC ATAAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833508.1
CDS	29..3673
Translation	MRSKGRARKLATNNECVYGSYPEIPLEEMPDADGVAGALSLSIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEAGEKFGPYVGEQRSNLKDPSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835206.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835206.1

ORF Insert Sequence:

ATGAGATCCA AAGGCAGGGC AAGGAAACTG GCCACAAATA ATGAGTGTGT ATATGGCAGC 
TACCCTGAAA TTCCTTTGGA AGAAATGCCA GATGCAGATG GAGTAGCTGG TGCTCTGTCC 
CTCAGTATTC AAGAGCCATG CTCTCCAGCC ACATCCAGTG AAGCGTTCAC TCCAAAGGAG 
GGCTCTCCTT ACAAAGCCCC CATCTACATC CCTGATGATA TCCCCATTCC TGCTGAGTTT 
GAACTTCGAG AGTCAAATAT GCCTGGAGCA GGCCTTGGAA TATGGACCAA AAGGAAAATT 
GAAGCAGGTG AAAAGTTTGG GCCTTATGTG GGAGAGCAGA GGTCAAACCT GAAAGACCCT 
AGTTACGGAT GGGAGATCTT AGATGAGTTT TGCAATGTGA AGTTCTGCAT AGATGCTAGT 
CAACCAGATG TAGGAAGCTG GCTCAAGTAC ATCAGGTTCG CTGGCTGTTA TGATCAGCAC 
AACCTCGTTG CATGCCAGAT AAATGATCAG ATATTTTATA GAGTAGTCGC AGACATTGCA 
CCAGGAGAGG AGCTCCTGCT GTTCATGAAG AGTGAAGACT ATTCCCATGA AACTATGGCA 
CCAGATATCC ACGAAGAACG GCAGTACCGC TGTGAGGACT GTGACCAGCT CTTTGAGTCC 
AAGGCCGAGC TAGCAGATCA CCAGAAGTTC CCATGCAGCA CTCCCCATTC GGCTTTCTCA 
ATGGTAGAAG ACGACTTCCA ACAAAAGCTC GAGAGTGAAA ATGATCTCCG AGAGATACAT 
GCGATTCAGG AGTGTAAGGA ATGTGACCAA GTGTTTCCCG ATTTGCAAAG CTTGGAGAAA 
CACATGTTGT CACATACTGA AGAGAGGGAA TACAAGTGCG ATCAGTGTCC CAAAGCATTT 
AACTGGAAGT CCAATTTAAT TCGCCACCAG ATGTCACATG ACAGTGGAAA GCACTATGAA 
TGTGAGAACT GCGCCAAGCA GGTTTTCACG GACCCTAGCA ACCTTCAGCG GCACATCCGC 
TCTCAGCATG TTGGTGCCCG GGCCCACGCG TGCCCCGAGT GTGGCAAAAC GTTTGCCACT 
TCGTCGGGCC TCAAACAACA CAAGCACATC CACAGCAGTG TGAAGCCCTT TATCTGTGAG 
GTCTGCCATA AATCCTATAC TCAGTTTTCA AACCTTTGTC GTCATAAGCG CATGCATGCT 
GATTGCAGAA CCCAAATCAA GTGCAAAGAC TGTGGACAAA TGTTCAGCAC TACGTCTTCC 
TTAAATAAAC ACAGGAGGTT TTGTGAGGGC AAGAACCATT TTGCGGCAGG TGGATTTTTT 
GGCCAAGGCA TTTCACTTCC TGGAACCCCA GCTATGGATA AAACGTCCAT GGTTAATATG 
AGTCATGCCA ACCCGGGCCT TGCTGACTAT TTTGGCGCCA ATAGGCATCC TGCTGGTCTT 
ACCTTTCCAA CAGCTCCTGG ATTTTCTTTT AGCTTCCCTG GTCTGTTTCC TTCCGGCTTG 
TACCACAGGC CTCCTTTGAT ACCTGCTAGT TCTCCTGTTA AAGGACTGTC AAGTACTGAA 
CAGACAAACA AAAGTCAAAG TCCCCTCATG ACACATCCTC AGATACTGCC AGCTACACAG 
GATATTTTGA AGGCACTATC TAAACACCCA CCTGTAGGGG ACAATAAGCC AGTGGAGCTC 
CAGCCCGAGA GGTCCTCTGA AGAGAGGCCC CTTGAGAAAA TCAGTGACCA GTCAGAGAGT 
AGTGACCTTG ATGATGTCAG TACGCCAAGT GGCAGTGACC TGGAAACAAC CTCGGGCTCT 
GATCTGGAAA GTGACATTGA AAGTGATAAA GAGAAATTTA AAGAAAATGG TAAAATGTTC 
AAAGACAAAG TAAGCCCTCT TCAGAATCTG GCTTCAATAC ATAATAAGAA AGAATACAGC 
AATCATTCCA TTTTCTCACC ATCTTTAGAG GAGCAGACTG CGGTGTCAGG AGCTGTGAAT 
GATTCTATAA AGGCTATTGC TTCTATTGCT GAAAAATACT TTGGTTCAAC AGGACTGGTG 
GGGCTGCAAG ACAAAAAAGT TGGAGCTTTA CCTTACCCTT CCATGTTTCC CCTCCCATTT 
TTTCCAGCAT TCTCTCAATC AATGTACCCA TTTCCTGATA GAGACTTGAG ATCGTTACCT 
TTGAAAATGG AGCCCCAATC ACCAAGTGAA GTAAAGAAAC TGCAGAAGGG AAGCTCTGAG 
TCTCCCTTTG ATCTCACCAC TAAACGCAAG GACGAGAAGC CCTTAACCCC AGTACCTGCC 
AAGCCTTCAG CGACCCCGGC TGCAAGCCAA GACCAGCCCC TGGATCTAAG TATGGGCAGT 
AGGAGTAGAG CCAGTGGAAC CAAGCTGACT GAGCCTCGTA AAAACCACGT GTTTGGGGAG 
AAGAAAGGAG GCAACATTGA ACCAAGACCA GCATCCGATG GTTCCTTACA GCATGCTAGA 
CCCACTCCTT TCTTTATGGA CCCTATTTAC AGAGTAGAGA AAAGAAAACT AACTGACCCA 
CTTGAAGCTT TAAAAGAGAA ATACTTGAGG CCTTCTCCAG GATTCTTATT TCACCCACAA 
TTCCAACTGC CTGATCAGAG AACTTGGATG TCGGCTATTG AAAACATGGC AGAGAAGCTG 
GAGAGCTTCA GTGCCCTGAA ACCAGAGGCC AGTGAACTTC TACAGTCTGT TCCCTCCATG 
TTCAACTTCA GAGCTCCTCC CAACGCCCTG CCAGAGAACC TCCTACGGAA AGGAAAGGAG 
CGCTATACCT GCAGATGCAA GTACTGTGAC AGGTCCTTCA GCATATCTTC CAACCTGCAG 
CGGCATGTTC GCAACATCCA CAATAAAGAG AAGCCCTTTA AGTGTCACTT ATGTGATAGG 
TGTTTTGGTC AACAAACCAA CTTAGACAGA CACCTCAAGA AGCACGAGAA TGGGAACATG 
TCTGGTACGG CAACATCATC GCCTCATTCT GAACTGGAAA GCACAGGTGC AATCCTCGAT 
GACAAGGAAG ATGCTTACTT CACAGAAATT CGAAATTTCA TCGGGAATAG CAACCACGGC 
AGCCAGTCCC CCAGGAACGC CGAAGAGAGA ATGAATGGCA GTCACTTTAA AGATGAAAAG 
GCTTTGGTGA CCAATCAAAA TTCAGACTTA CTGGATGATG AAGAAGTAGA AGATGAGGTG 
TTGTTAGATG AGGAGGATGA AGACAATGAT ATTACTGGGA AAATGGGAAA GGAACCAGGG 
ACGAGTAATT TACATGAAGG AAACCCCGAG GATGACTATG AAGACACCAG CACCCTGGAA 
ATGAGCTGTA AAGCATCCCC AATGAGGTAT AAAGAGGAAG AGTATAAAAC TGGACTTTCT 
GCTCTAGATC ATATAAGGCA CTTCACAGAT AGCCTCAAAA TGAGGAAAAT GGAAGATAAT 
CAATATACAG AAGCTGAGGT GTCTTCTTTC AGTGCTTCCC ATGTGCCAGA GGAACTTAAA 
CAACCGTTAC ACAGAAAGTC CAAGTCACAG GCATACGCTA TGATGTTGTC ATTGTCCGAC 
AAGGAGTCCC TGCATTCTTC ATCCCACAGT TCTTCCAACG TGTGGCATAG TATGGCCAGG 
GCCGCAGCAG AATCCAGTGC CATCCAGTCC ATAAGCCACG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835207.1 ) cDNA ORF clone, Bison bison bison -> XP_010833509.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBi61228
Clone ID Related Accession (Same CDS sequence)	XM_010835207.1
Accession Version	XM_010835207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATG CAGATGGAGT AGCTGGTGCT CTGTCCCTCA GTATTCAAGA GCCATGCTCT 
CCAGCCACAT CCAGTGAAGC GTTCACTCCA AAGGAGGGCT CTCCTTACAA AGCCCCCATC 
TACATCCCTG ATGATATCCC CATTCCTGCT GAGTTTGAAC TTCGAGAGTC AAATATGCCT 
GGAGCAGGCC TTGGAATATG GACCAAAAGG AAAATTGAAG CAGGTGAAAA GTTTGGGCCT 
TATGTGGGAG AGCAGAGGTC AAACCTGAAA GACCCTAGTT ACGGATGGGA GATCTTAGAT 
GAGTTTTGCA ATGTGAAGTT CTGCATAGAT GCTAGTCAAC CAGATGTAGG AAGCTGGCTC 
AAGTACATCA GGTTCGCTGG CTGTTATGAT CAGCACAACC TCGTTGCATG CCAGATAAAT 
GATCAGATAT TTTATAGAGT AGTCGCAGAC ATTGCACCAG GAGAGGAGCT CCTGCTGTTC 
ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833509.1
CDS	80..3718
Translation	MPDADGVAGALSLSIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEAGEKFGPYVGEQRSNLKDPSYGWEILDEFCNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835207.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835207.1

ORF Insert Sequence:

ATGCCAGATG CAGATGGAGT AGCTGGTGCT CTGTCCCTCA GTATTCAAGA GCCATGCTCT 
CCAGCCACAT CCAGTGAAGC GTTCACTCCA AAGGAGGGCT CTCCTTACAA AGCCCCCATC 
TACATCCCTG ATGATATCCC CATTCCTGCT GAGTTTGAAC TTCGAGAGTC AAATATGCCT 
GGAGCAGGCC TTGGAATATG GACCAAAAGG AAAATTGAAG CAGGTGAAAA GTTTGGGCCT 
TATGTGGGAG AGCAGAGGTC AAACCTGAAA GACCCTAGTT ACGGATGGGA GATCTTAGAT 
GAGTTTTGCA ATGTGAAGTT CTGCATAGAT GCTAGTCAAC CAGATGTAGG AAGCTGGCTC 
AAGTACATCA GGTTCGCTGG CTGTTATGAT CAGCACAACC TCGTTGCATG CCAGATAAAT 
GATCAGATAT TTTATAGAGT AGTCGCAGAC ATTGCACCAG GAGAGGAGCT CCTGCTGTTC 
ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835209.1 ) cDNA ORF clone, Bison bison bison -> XP_010833511.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBi61229
Clone ID Related Accession (Same CDS sequence)	XM_010835209.1 , XM_010835208.1 , XM_010835210.1
Accession Version	XM_010835209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833511.1
CDS	666..3824
Translation	MKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835209.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835209.1

ORF Insert Sequence:

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835208.1 ) cDNA ORF clone, Bison bison bison -> XP_010833510.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBi61229
Clone ID Related Accession (Same CDS sequence)	XM_010835209.1 , XM_010835208.1 , XM_010835210.1
Accession Version	XM_010835208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833510.1
CDS	364..3522
Translation	MKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835208.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835208.1

ORF Insert Sequence:

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MECOM ( XM_010835210.1 ) cDNA ORF clone, Bison bison bison -> XP_010833512.1 Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X8, mRNA.

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CloneID	OBi61229
Clone ID Related Accession (Same CDS sequence)	XM_010835209.1 , XM_010835208.1 , XM_010835210.1
Accession Version	XM_010835210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	MDS1 and EVI1 complex locus protein EVI1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494612.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010833512.1
CDS	383..3541
Translation	MKSEDYSHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEDDFQQKLESENDLREIHAIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKQVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPPVGDNKPVELQPERSSEERPLEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASIHNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPSEVKKLQKGSSESPFDLTTKRKDEKPLTPVPAKPSATPAASQDQPLDLSMGSRSRASGTKLTEPRKNHVFGEKKGGNIEPRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQFQLPDQRTWMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNAEERMNGSHFKDEKALVTNQNSDLLDDEEVEDEVLLDEEDEDNDITGKMGKEPGTSNLHEGNPEDDYEDTSTLEMSCKASPMRYKEEEYKTGLSALDHIRHFTDSLKMRKMEDNQYTEAEVSSFSASHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSSSHSSSNVWHSMARAAAESSAIQSISHV

Target ORF information:

RefSeq Version	XM_010835210.1
Organism	Bison bison bison
Definition	Bison bison bison MDS1 and EVI1 complex locus (MECOM), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010835210.1

ORF Insert Sequence:

ATGAAGAGTG AAGACTATTC CCATGAAACT ATGGCACCAG ATATCCACGA AGAACGGCAG 
TACCGCTGTG AGGACTGTGA CCAGCTCTTT GAGTCCAAGG CCGAGCTAGC AGATCACCAG 
AAGTTCCCAT GCAGCACTCC CCATTCGGCT TTCTCAATGG TAGAAGACGA CTTCCAACAA 
AAGCTCGAGA GTGAAAATGA TCTCCGAGAG ATACATGCGA TTCAGGAGTG TAAGGAATGT 
GACCAAGTGT TTCCCGATTT GCAAAGCTTG GAGAAACACA TGTTGTCACA TACTGAAGAG 
AGGGAATACA AGTGCGATCA GTGTCCCAAA GCATTTAACT GGAAGTCCAA TTTAATTCGC 
CACCAGATGT CACATGACAG TGGAAAGCAC TATGAATGTG AGAACTGCGC CAAGCAGGTT 
TTCACGGACC CTAGCAACCT TCAGCGGCAC ATCCGCTCTC AGCATGTTGG TGCCCGGGCC 
CACGCGTGCC CCGAGTGTGG CAAAACGTTT GCCACTTCGT CGGGCCTCAA ACAACACAAG 
CACATCCACA GCAGTGTGAA GCCCTTTATC TGTGAGGTCT GCCATAAATC CTATACTCAG 
TTTTCAAACC TTTGTCGTCA TAAGCGCATG CATGCTGATT GCAGAACCCA AATCAAGTGC 
AAAGACTGTG GACAAATGTT CAGCACTACG TCTTCCTTAA ATAAACACAG GAGGTTTTGT 
GAGGGCAAGA ACCATTTTGC GGCAGGTGGA TTTTTTGGCC AAGGCATTTC ACTTCCTGGA 
ACCCCAGCTA TGGATAAAAC GTCCATGGTT AATATGAGTC ATGCCAACCC GGGCCTTGCT 
GACTATTTTG GCGCCAATAG GCATCCTGCT GGTCTTACCT TTCCAACAGC TCCTGGATTT 
TCTTTTAGCT TCCCTGGTCT GTTTCCTTCC GGCTTGTACC ACAGGCCTCC TTTGATACCT 
GCTAGTTCTC CTGTTAAAGG ACTGTCAAGT ACTGAACAGA CAAACAAAAG TCAAAGTCCC 
CTCATGACAC ATCCTCAGAT ACTGCCAGCT ACACAGGATA TTTTGAAGGC ACTATCTAAA 
CACCCACCTG TAGGGGACAA TAAGCCAGTG GAGCTCCAGC CCGAGAGGTC CTCTGAAGAG 
AGGCCCCTTG AGAAAATCAG TGACCAGTCA GAGAGTAGTG ACCTTGATGA TGTCAGTACG 
CCAAGTGGCA GTGACCTGGA AACAACCTCG GGCTCTGATC TGGAAAGTGA CATTGAAAGT 
GATAAAGAGA AATTTAAAGA AAATGGTAAA ATGTTCAAAG ACAAAGTAAG CCCTCTTCAG 
AATCTGGCTT CAATACATAA TAAGAAAGAA TACAGCAATC ATTCCATTTT CTCACCATCT 
TTAGAGGAGC AGACTGCGGT GTCAGGAGCT GTGAATGATT CTATAAAGGC TATTGCTTCT 
ATTGCTGAAA AATACTTTGG TTCAACAGGA CTGGTGGGGC TGCAAGACAA AAAAGTTGGA 
GCTTTACCTT ACCCTTCCAT GTTTCCCCTC CCATTTTTTC CAGCATTCTC TCAATCAATG 
TACCCATTTC CTGATAGAGA CTTGAGATCG TTACCTTTGA AAATGGAGCC CCAATCACCA 
AGTGAAGTAA AGAAACTGCA GAAGGGAAGC TCTGAGTCTC CCTTTGATCT CACCACTAAA 
CGCAAGGACG AGAAGCCCTT AACCCCAGTA CCTGCCAAGC CTTCAGCGAC CCCGGCTGCA 
AGCCAAGACC AGCCCCTGGA TCTAAGTATG GGCAGTAGGA GTAGAGCCAG TGGAACCAAG 
CTGACTGAGC CTCGTAAAAA CCACGTGTTT GGGGAGAAGA AAGGAGGCAA CATTGAACCA 
AGACCAGCAT CCGATGGTTC CTTACAGCAT GCTAGACCCA CTCCTTTCTT TATGGACCCT 
ATTTACAGAG TAGAGAAAAG AAAACTAACT GACCCACTTG AAGCTTTAAA AGAGAAATAC 
TTGAGGCCTT CTCCAGGATT CTTATTTCAC CCACAATTCC AACTGCCTGA TCAGAGAACT 
TGGATGTCGG CTATTGAAAA CATGGCAGAG AAGCTGGAGA GCTTCAGTGC CCTGAAACCA 
GAGGCCAGTG AACTTCTACA GTCTGTTCCC TCCATGTTCA ACTTCAGAGC TCCTCCCAAC 
GCCCTGCCAG AGAACCTCCT ACGGAAAGGA AAGGAGCGCT ATACCTGCAG ATACTGTGGT 
AAGATTTTTC CAAGATCTGC GAACCTAACA CGGCACTTGA GAACCCACAC AGGAGAGCAG 
CCTTATAGAT GCAAGTACTG TGACAGGTCC TTCAGCATAT CTTCCAACCT GCAGCGGCAT 
GTTCGCAACA TCCACAATAA AGAGAAGCCC TTTAAGTGTC ACTTATGTGA TAGGTGTTTT 
GGTCAACAAA CCAACTTAGA CAGACACCTC AAGAAGCACG AGAATGGGAA CATGTCTGGT 
ACGGCAACAT CATCGCCTCA TTCTGAACTG GAAAGCACAG GTGCAATCCT CGATGACAAG 
GAAGATGCTT ACTTCACAGA AATTCGAAAT TTCATCGGGA ATAGCAACCA CGGCAGCCAG 
TCCCCCAGGA ACGCCGAAGA GAGAATGAAT GGCAGTCACT TTAAAGATGA AAAGGCTTTG 
GTGACCAATC AAAATTCAGA CTTACTGGAT GATGAAGAAG TAGAAGATGA GGTGTTGTTA 
GATGAGGAGG ATGAAGACAA TGATATTACT GGGAAAATGG GAAAGGAACC AGGGACGAGT 
AATTTACATG AAGGAAACCC CGAGGATGAC TATGAAGACA CCAGCACCCT GGAAATGAGC 
TGTAAAGCAT CCCCAATGAG GTATAAAGAG GAAGAGTATA AAACTGGACT TTCTGCTCTA 
GATCATATAA GGCACTTCAC AGATAGCCTC AAAATGAGGA AAATGGAAGA TAATCAATAT 
ACAGAAGCTG AGGTGTCTTC TTTCAGTGCT TCCCATGTGC CAGAGGAACT TAAACAACCG 
TTACACAGAA AGTCCAAGTC ACAGGCATAC GCTATGATGT TGTCATTGTC CGACAAGGAG 
TCCCTGCATT CTTCATCCCA CAGTTCTTCC AACGTGTGGC ATAGTATGGC CAGGGCCGCA 
GCAGAATCCA GTGCCATCCA GTCCATAAGC CACGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MECOM cDNA ORF clone, Bison bison bison

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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