ECT2 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECT2
Entrez Gene ID	104639765
Full Name	epithelial cell transforming 2
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010307916.1	XP_010306218.1	protein ECT2 isoform X1
XM_010307917.1	XP_010306219.1	protein ECT2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ECT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa189207	XM_010307916.1 Latest version!	Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBa189208	XM_010307917.1 Latest version!	Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECT2 ( XM_010307916.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010306218.1 Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa189207
Clone ID Related Accession (Same CDS sequence)	XM_010307916.1
Accession Version	XM_010307916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	protein ECT2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010769728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA ACAGTACGCT GGTGTCTGAA ACCGGGAGGA GCCTCTTGGC TGATACTTCT 
GTTCTTGATT CAAAAATTAT CGAAACCTCC AAAGAGAATG TATTTCCTGG ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA AGAAGCAGGG 
AAACGTGAGG AGCTTTTGAA AGCCTTGGAG GAAATTAAAG TACCCTACAT AAAGACAGAC 
ACAATAGAAG AGCTTGGAGA TGCTGATTCC CCAGAATTTG AGACTATCTT CGTTGTATCA 
GACTTCCAAG ATTCTACCTT TAACAACCTC TGCAAAGCAG ATTGCCGTGT CATTGGCCCT 
CCAGTAGTAC TGCACTGTGC ACAAAAAGGA GAGGCTTTAC CTTTCTCCTG TCGTCCACTG 
TACTGTGCAA GTATGTTGAA CTTGGTACTG TGCTTTACAG GATTCAGAAA GAAAGATGAA 
TTAGTTAAGC TAGTGACCTT GGTTCATCAC ATGGGTGGGA TTATTCGAAG GGACTTCAGC 
TCAAAAGTTA CTCATCTGGT GGCTAGCTCA ACACATGGAG ACAAATTTAG AATTGCCGTA 
AGTCTTGGTA CTCCTATCAT GAAAGCTGAG TGGATTTATA AGGCTTGGGA GAAAAGGAAT 
GAAATTGATT TCTGTGCAGC TGATGATGAC TTTAGAAATC AGTTCAAGGT TCCTCCCTTC 
CAGGATTGCA TGTTAAGTTT CCTTGGATTC TCTGATGATG AGAGAGCTAA CATGGAAGAA 
ATGACAGAAA TGCAAGGAGG ACATTATTTA CCAGTTGGTG ATGAAAGGTG TACGCACTTA 
GTGGTTGAAG AAAGTACAGT AAAAGATCTT CCATTTGAGC CTTTAAAAAA GCTTTATGTT 
GTAAAGCAAG AGTGGTTCTG GGGAAGCATT CAGATGGATG CCAGAGCTGG AGAGTCCATG 
TATTTATTTG AGAAGTCAGA GAGTCCTGGC CTCAAGAAGT CTGTGTCGCT ACTTTCTCTG 
AATACTCCAA ACAGCAATCG TAAAAGACGC CGACTAAAAG AAACTCTCGC ACAGCTGACT 
AGAGAAACAG ACATGTCGCC CTTTCCTCCT CGTAAGCGCC CATCGGCTGA ACATTCGCTT 
TCAATTGGGT CCTTGCTGGA TATTTCCAAC ACTCCAGAGT CGGGCACTGC CAGCGGAGAA 
ACACCAAAAT CCTGTGCAAG ACCTTCCAAA AACTCAACGC CTCTTCCACT AAAGCAGTCT 
GCAAGGTGGC AGGTTGCAAA GGAATTGTAT CAGACAGAAA GTAACTATGT TGATATTCTA 
ACAACAATCA TTCAGTTATT TCAGGTTCCG TTGGAGAAAG AAGGACAACT TGGAGGACCA 
ATCCTTGCAC AGGAAGAGAT TAAGACCATA TTTGGCAGCA TTCCAGATAT TCTTGATGTG 
CATATTAAAA TCAAGGAAGA CCTGGAAGAT CTTATGATTA ATTGGACTGA GAACAAAAGT 
ATTGGTGATA TCATTCTTAA ATATTCAAAA GACTTGTTGA AAACATACCC TCCATTTGTG 
AATTTCTTTG AAATGAGCAA GGAGACTATA ACTAGATGTG AAAAACAGAA GCCAAGGTTT 
CATGCCTTCC TAAAGATAAA CCAAGCTAAA CCTGAATGTG GCCGCCAAAG CCTAGCTGAA 
CTCCTTATCC GTCCTGTTCA AAGGCTGCCT AGTGTTGCTC TACTACTAAA TGATATTAAG 
AAGCATACAG CAGAAGAGAA CCCAGATAAA GTAACTCTAG AGAGAGCTAT TGAATCATTA 
AAGGAAGTGA TGACACATAT TAATGAAGAC AAAAGAAAAA CAGAGGCACA AAAGCAGTTC 
TTTGACGTTG TCTATGAAGT TGACGGGTGT CCAGCCAATC TCTTGTCCTC TCATCGAAGC 
TTAGTTCAGC GTTTGGAAAC TGTAGCACTT GGCGATGACC TCTGTGACAG GGGAGAGCAG 
GTCACACTTT TTCTGTTCAA TGATTGCCTA GAGATTGCAA GGAAACGGCA TAAAGTCATT 
GGTGCTTTCA AGAGTCCGCA TGGCCACACA AGGCCTCCTG CGTCTCTCAA GCATGTTGTT 
CTCATGCCTC TCTCCCAGAT CAAGAAAGTC CTAGACATCA GGGAGACAGA AGATTGCCAT 
AAAGCTTTTG CCTTAGTTGT GCGGCCACCT ACAGAACTTA ACAACAAGCT ACTCAGCTTC 
CAGATGACAA CTGAAGAACC TCCTAAAGAA GATTGGTTGA AGATGTTGTG TCGGCATGTG 
GCTAACACTA TTTGTAAAGC AGATGCAGAA AATCTCATTT ATACTGCTGA TCCTGATTCA 
GTTGAAGTAA ATACTAAGGA CATGGACAGT ACTTTAAGCA GAGCATCCAG GGCAATAAAG 
AAAACATCAA AAAAGGTTAC AAGAGCATTT TCTTTCTCAA AAACACCAAA GAGAGCTCTT 
CGAAGGGCTT TAATGTCACA TAGTGCAACA GAAGGAAGAA GTCCCAGCTC AGCTAACGAG 
AGTTATATAG GCAGCCGACT GACTAGTACG TCATCATTAG CGGGTATTCC TTCTCCTTCC 
TTGGTCAGTC TTCCCTCAAT CTTTGAAAAA AGGAGTCATA CATTAAGTCG ATCAACAACC 
CACTTGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010306218.1
CDS	1..2652
Translation	MADNSTLVSETGRSLLADTSVLDSKIIETSKENVFPGSVLDVEEEMPQIETRVVLVQEAGKREELLKALEEIKVPYIKTDTIEELGDADSPEFETIFVVSDFQDSTFNNLCKADCRVIGPPVVLHCAQKGEALPFSCRPLYCASMLNLVLCFTGFRKKDELVKLVTLVHHMGGIIRRDFSSKVTHLVASSTHGDKFRIAVSLGTPIMKAEWIYKAWEKRNEIDFCAADDDFRNQFKVPPFQDCMLSFLGFSDDERANMEEMTEMQGGHYLPVGDERCTHLVVEESTVKDLPFEPLKKLYVVKQEWFWGSIQMDARAGESMYLFEKSESPGLKKSVSLLSLNTPNSNRKRRRLKETLAQLTRETDMSPFPPRKRPSAEHSLSIGSLLDISNTPESGTASGETPKSCARPSKNSTPLPLKQSARWQVAKELYQTESNYVDILTTIIQLFQVPLEKEGQLGGPILAQEEIKTIFGSIPDILDVHIKIKEDLEDLMINWTENKSIGDIILKYSKDLLKTYPPFVNFFEMSKETITRCEKQKPRFHAFLKINQAKPECGRQSLAELLIRPVQRLPSVALLLNDIKKHTAEENPDKVTLERAIESLKEVMTHINEDKRKTEAQKQFFDVVYEVDGCPANLLSSHRSLVQRLETVALGDDLCDRGEQVTLFLFNDCLEIARKRHKVIGAFKSPHGHTRPPASLKHVVLMPLSQIKKVLDIRETEDCHKAFALVVRPPTELNNKLLSFQMTTEEPPKEDWLKMLCRHVANTICKADAENLIYTADPDSVEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMSHSATEGRSPSSANESYIGSRLTSTSSLAGIPSPSLVSLPSIFEKRSHTLSRSTTHLI

Target ORF information:

RefSeq Version	XM_010307916.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010307916.1

ORF Insert Sequence:

ATGGCTGACA ACAGTACGCT GGTGTCTGAA ACCGGGAGGA GCCTCTTGGC TGATACTTCT 
GTTCTTGATT CAAAAATTAT CGAAACCTCC AAAGAGAATG TATTTCCTGG ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA AGAAGCAGGG 
AAACGTGAGG AGCTTTTGAA AGCCTTGGAG GAAATTAAAG TACCCTACAT AAAGACAGAC 
ACAATAGAAG AGCTTGGAGA TGCTGATTCC CCAGAATTTG AGACTATCTT CGTTGTATCA 
GACTTCCAAG ATTCTACCTT TAACAACCTC TGCAAAGCAG ATTGCCGTGT CATTGGCCCT 
CCAGTAGTAC TGCACTGTGC ACAAAAAGGA GAGGCTTTAC CTTTCTCCTG TCGTCCACTG 
TACTGTGCAA GTATGTTGAA CTTGGTACTG TGCTTTACAG GATTCAGAAA GAAAGATGAA 
TTAGTTAAGC TAGTGACCTT GGTTCATCAC ATGGGTGGGA TTATTCGAAG GGACTTCAGC 
TCAAAAGTTA CTCATCTGGT GGCTAGCTCA ACACATGGAG ACAAATTTAG AATTGCCGTA 
AGTCTTGGTA CTCCTATCAT GAAAGCTGAG TGGATTTATA AGGCTTGGGA GAAAAGGAAT 
GAAATTGATT TCTGTGCAGC TGATGATGAC TTTAGAAATC AGTTCAAGGT TCCTCCCTTC 
CAGGATTGCA TGTTAAGTTT CCTTGGATTC TCTGATGATG AGAGAGCTAA CATGGAAGAA 
ATGACAGAAA TGCAAGGAGG ACATTATTTA CCAGTTGGTG ATGAAAGGTG TACGCACTTA 
GTGGTTGAAG AAAGTACAGT AAAAGATCTT CCATTTGAGC CTTTAAAAAA GCTTTATGTT 
GTAAAGCAAG AGTGGTTCTG GGGAAGCATT CAGATGGATG CCAGAGCTGG AGAGTCCATG 
TATTTATTTG AGAAGTCAGA GAGTCCTGGC CTCAAGAAGT CTGTGTCGCT ACTTTCTCTG 
AATACTCCAA ACAGCAATCG TAAAAGACGC CGACTAAAAG AAACTCTCGC ACAGCTGACT 
AGAGAAACAG ACATGTCGCC CTTTCCTCCT CGTAAGCGCC CATCGGCTGA ACATTCGCTT 
TCAATTGGGT CCTTGCTGGA TATTTCCAAC ACTCCAGAGT CGGGCACTGC CAGCGGAGAA 
ACACCAAAAT CCTGTGCAAG ACCTTCCAAA AACTCAACGC CTCTTCCACT AAAGCAGTCT 
GCAAGGTGGC AGGTTGCAAA GGAATTGTAT CAGACAGAAA GTAACTATGT TGATATTCTA 
ACAACAATCA TTCAGTTATT TCAGGTTCCG TTGGAGAAAG AAGGACAACT TGGAGGACCA 
ATCCTTGCAC AGGAAGAGAT TAAGACCATA TTTGGCAGCA TTCCAGATAT TCTTGATGTG 
CATATTAAAA TCAAGGAAGA CCTGGAAGAT CTTATGATTA ATTGGACTGA GAACAAAAGT 
ATTGGTGATA TCATTCTTAA ATATTCAAAA GACTTGTTGA AAACATACCC TCCATTTGTG 
AATTTCTTTG AAATGAGCAA GGAGACTATA ACTAGATGTG AAAAACAGAA GCCAAGGTTT 
CATGCCTTCC TAAAGATAAA CCAAGCTAAA CCTGAATGTG GCCGCCAAAG CCTAGCTGAA 
CTCCTTATCC GTCCTGTTCA AAGGCTGCCT AGTGTTGCTC TACTACTAAA TGATATTAAG 
AAGCATACAG CAGAAGAGAA CCCAGATAAA GTAACTCTAG AGAGAGCTAT TGAATCATTA 
AAGGAAGTGA TGACACATAT TAATGAAGAC AAAAGAAAAA CAGAGGCACA AAAGCAGTTC 
TTTGACGTTG TCTATGAAGT TGACGGGTGT CCAGCCAATC TCTTGTCCTC TCATCGAAGC 
TTAGTTCAGC GTTTGGAAAC TGTAGCACTT GGCGATGACC TCTGTGACAG GGGAGAGCAG 
GTCACACTTT TTCTGTTCAA TGATTGCCTA GAGATTGCAA GGAAACGGCA TAAAGTCATT 
GGTGCTTTCA AGAGTCCGCA TGGCCACACA AGGCCTCCTG CGTCTCTCAA GCATGTTGTT 
CTCATGCCTC TCTCCCAGAT CAAGAAAGTC CTAGACATCA GGGAGACAGA AGATTGCCAT 
AAAGCTTTTG CCTTAGTTGT GCGGCCACCT ACAGAACTTA ACAACAAGCT ACTCAGCTTC 
CAGATGACAA CTGAAGAACC TCCTAAAGAA GATTGGTTGA AGATGTTGTG TCGGCATGTG 
GCTAACACTA TTTGTAAAGC AGATGCAGAA AATCTCATTT ATACTGCTGA TCCTGATTCA 
GTTGAAGTAA ATACTAAGGA CATGGACAGT ACTTTAAGCA GAGCATCCAG GGCAATAAAG 
AAAACATCAA AAAAGGTTAC AAGAGCATTT TCTTTCTCAA AAACACCAAA GAGAGCTCTT 
CGAAGGGCTT TAATGTCACA TAGTGCAACA GAAGGAAGAA GTCCCAGCTC AGCTAACGAG 
AGTTATATAG GCAGCCGACT GACTAGTACG TCATCATTAG CGGGTATTCC TTCTCCTTCC 
TTGGTCAGTC TTCCCTCAAT CTTTGAAAAA AGGAGTCATA CATTAAGTCG ATCAACAACC 
CACTTGATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECT2 ( XM_010307917.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010306219.1 Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa189208
Clone ID Related Accession (Same CDS sequence)	XM_010307917.1
Accession Version	XM_010307917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	protein ECT2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010769728.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGACA ACAGTACGCT GGTGTCTGAA ACCGGGAGGA GCCTCTTGGC TGATACTTCT 
GTTCTTGATT CAAAAATTAT CGAAACCTCC AAAGAGAATG TATTTCCTGG ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA AGAAGCAGGG 
AAACGTGAGG AGCTTTTGAA AGCCTTGGAG ACCATTAAGA TAATGGAAGT ACCAGTTATA 
AAGATAAAGG AAACTAGTCC TGGTAAATCA GAAGAAAAAC TAATAAAAAG TATTATTCAT 
ATGGAAATTA AAGTACCCTA CATAAAGACA GACACAATAG AAGAGCTTGG AGATGCTGAT 
TCCCCAGAAT TTGAGACTAT CTTCGTTGTA TCAGACTTCC AAGATTCTAC CTTTAACAAC 
CTCTGCAAAG CAGATTGCCG TGTCATTGGC CCTCCAGTAG TACTGCACTG TGCACAAAAA 
GGAGAGGCTT TACCTTTCTC CTGTCGTCCA CTGTACTGTG CAAGTATGTT GAACTTGGTA 
CTGTGCTTTA CAGGATTCAG AAAGAAAGAT GAATTAGTTA AGCTAGTGAC CTTGGTTCAT 
CACATGGGTG GGATTATTCG AAGGGACTTC AGCTCAAAAG TTACTCATCT GGTGGCTAGC 
TCAACACATG GAGACAAATT TAGAATTGCC GTAAGTCTTG GTACTCCTAT CATGAAAGCT 
GAGTGGATTT ATAAGGCTTG GGAGAAAAGG AATGAAATTG ATTTCTGTGC AGCTGATGAT 
GACTTTAGAA ATCAGTTCAA GGTTCCTCCC TTCCAGGATT GCATGTTAAG TTTCCTTGGA 
TTCTCTGATG ATGAGAGAGC TAACATGGAA GAAATGACAG AAATGCAAGG AGGACATTAT 
TTACCAGTTG GTGATGAAAG GTGTACGCAC TTAGTGGTTG AAGAAAGTAC AGTAAAAGAT 
CTTCCATTTG AGCCTTTAAA AAAGCTTTAT GTTGTAAAGC AAGAGTGGTT CTGGGGAAGC 
ATTCAGATGG ATGCCAGAGC TGGAGAGTCC ATGTATTTAT TTGAGAAGTC AGAGAGTCCT 
GGCCTCAAGA AGTCTGTGTC GCTACTTTCT CTGAATACTC CAAACAGCAA TCGTAAAAGA 
CGCCGACTAA AAGAAACTCT CGCACAGCTG ACTAGAGAAA CAGACATGTC GCCCTTTCCT 
CCTCGTAAGC GCCCATCGGC TGAACATTCG CTTTCAATTG GGTCCTTGCT GGATATTTCC 
AACACTCCAG AGTCGGGCAC TGCCAGCGGA GAAACACCAA AATCCTGTGC AAGACCTTCC 
AAAAACTCAA CGCCTCTTCC ACTAAAGCAG TCTGCAAGGT GGCAGGTTGC AAAGGAATTG 
TATCAGACAG AAAGTAACTA TGTTGATATT CTAACAACAA TCATTCAGTT ATTTCAGGTT 
CCGTTGGAGA AAGAAGGACA ACTTGGAGGA CCAATCCTTG CACAGGAAGA GATTAAGACC 
ATATTTGGCA GCATTCCAGA TATTCTTGAT GTGCATATTA AAATCAAGGA AGACCTGGAA 
GATCTTATGA TTAATTGGAC TGAGAACAAA AGTATTGGTG ATATCATTCT TAAATATTCA 
AAAGACTTGT TGAAAACATA CCCTCCATTT GTGAATTTCT TTGAAATGAG CAAGGAGACT 
ATAACTAGAT GTGAAAAACA GAAGCCAAGG TTTCATGCCT TCCTAAAGAT AAACCAAGCT 
AAACCTGAAT GTGGCCGCCA AAGCCTAGCT GAACTCCTTA TCCGTCCTGT TCAAAGGCTG 
CCTAGTGTTG CTCTACTACT AAATGATATT AAGAAGCATA CAGCAGAAGA GAACCCAGAT 
AAAGTAACTC TAGAGAGAGC TATTGAATCA TTAAAGGAAG TGATGACACA TATTAATGAA 
GACAAAAGAA AAACAGAGGC ACAAAAGCAG TTCTTTGACG TTGTCTATGA AGTTGACGGG 
TGTCCAGCCA ATCTCTTGTC CTCTCATCGA AGCTTAGTTC AGCGTTTGGA AACTGTAGCA 
CTTGGCGATG ACCTCTGTGA CAGGGGAGAG CAGGTCACAC TTTTTCTGTT CAATGATTGC 
CTAGAGATTG CAAGGAAACG GCATAAAGTC ATTGGTGCTT TCAAGAGTCC GCATGGCCAC 
ACAAGGCCTC CTGCGTCTCT CAAGCATGTT GTTCTCATGC CTCTCTCCCA GATCAAGAAA 
GTCCTAGACA TCAGGGAGAC AGAAGATTGC CATAAAGCTT TTGCCTTAGT TGTGCGGCCA 
CCTACAGAAC TTAACAACAA GCTACTCAGC TTCCAGATGA CAACTGAAGA ACCTCCTAAA 
GAAGATTGGT TGAAGATGTT GTGTCGGCAT GTGGCTAACA CTATTTGTAA AGCAGATGCA 
GAAAATCTCA TTTATACTGC TGATCCTGAT TCAGTTGAAG TAAATACTAA GGACATGGAC 
AGTACTTTAA GCAGAGCATC CAGGGCAATA AAGAAAACAT CAAAAAAGGT TACAAGAGCA 
TTTTCTTTCT CAAAAACACC AAAGAGAGCT CTTCGAAGGG CTTTAATGTC ACATAGTGCA 
ACAGAAGGAA GAAGTCCCAG CTCAGCTAAC GAGAGTTATA TAGGCAGCCG ACTGACTAGT 
ACGTCATCAT TAGCGGGTAT TCCTTCTCCT TCCTTGGTCA GTCTTCCCTC AATCTTTGAA 
AAAAGGAGTC ATACATTAAG TCGATCAACA ACCCACTTGA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010306219.1
CDS	16..2760
Translation	MADNSTLVSETGRSLLADTSVLDSKIIETSKENVFPGSVLDVEEEMPQIETRVVLVQEAGKREELLKALETIKIMEVPVIKIKETSPGKSEEKLIKSIIHMEIKVPYIKTDTIEELGDADSPEFETIFVVSDFQDSTFNNLCKADCRVIGPPVVLHCAQKGEALPFSCRPLYCASMLNLVLCFTGFRKKDELVKLVTLVHHMGGIIRRDFSSKVTHLVASSTHGDKFRIAVSLGTPIMKAEWIYKAWEKRNEIDFCAADDDFRNQFKVPPFQDCMLSFLGFSDDERANMEEMTEMQGGHYLPVGDERCTHLVVEESTVKDLPFEPLKKLYVVKQEWFWGSIQMDARAGESMYLFEKSESPGLKKSVSLLSLNTPNSNRKRRRLKETLAQLTRETDMSPFPPRKRPSAEHSLSIGSLLDISNTPESGTASGETPKSCARPSKNSTPLPLKQSARWQVAKELYQTESNYVDILTTIIQLFQVPLEKEGQLGGPILAQEEIKTIFGSIPDILDVHIKIKEDLEDLMINWTENKSIGDIILKYSKDLLKTYPPFVNFFEMSKETITRCEKQKPRFHAFLKINQAKPECGRQSLAELLIRPVQRLPSVALLLNDIKKHTAEENPDKVTLERAIESLKEVMTHINEDKRKTEAQKQFFDVVYEVDGCPANLLSSHRSLVQRLETVALGDDLCDRGEQVTLFLFNDCLEIARKRHKVIGAFKSPHGHTRPPASLKHVVLMPLSQIKKVLDIRETEDCHKAFALVVRPPTELNNKLLSFQMTTEEPPKEDWLKMLCRHVANTICKADAENLIYTADPDSVEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMSHSATEGRSPSSANESYIGSRLTSTSSLAGIPSPSLVSLPSIFEKRSHTLSRSTTHLI

Target ORF information:

RefSeq Version	XM_010307917.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps epithelial cell transforming 2 (ECT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010307917.1

ORF Insert Sequence:

ATGGCTGACA ACAGTACGCT GGTGTCTGAA ACCGGGAGGA GCCTCTTGGC TGATACTTCT 
GTTCTTGATT CAAAAATTAT CGAAACCTCC AAAGAGAATG TATTTCCTGG ATCTGTTTTG 
GATGTTGAAG AAGAAATGCC TCAAATAGAA ACAAGAGTGG TTTTGGTTCA AGAAGCAGGG 
AAACGTGAGG AGCTTTTGAA AGCCTTGGAG ACCATTAAGA TAATGGAAGT ACCAGTTATA 
AAGATAAAGG AAACTAGTCC TGGTAAATCA GAAGAAAAAC TAATAAAAAG TATTATTCAT 
ATGGAAATTA AAGTACCCTA CATAAAGACA GACACAATAG AAGAGCTTGG AGATGCTGAT 
TCCCCAGAAT TTGAGACTAT CTTCGTTGTA TCAGACTTCC AAGATTCTAC CTTTAACAAC 
CTCTGCAAAG CAGATTGCCG TGTCATTGGC CCTCCAGTAG TACTGCACTG TGCACAAAAA 
GGAGAGGCTT TACCTTTCTC CTGTCGTCCA CTGTACTGTG CAAGTATGTT GAACTTGGTA 
CTGTGCTTTA CAGGATTCAG AAAGAAAGAT GAATTAGTTA AGCTAGTGAC CTTGGTTCAT 
CACATGGGTG GGATTATTCG AAGGGACTTC AGCTCAAAAG TTACTCATCT GGTGGCTAGC 
TCAACACATG GAGACAAATT TAGAATTGCC GTAAGTCTTG GTACTCCTAT CATGAAAGCT 
GAGTGGATTT ATAAGGCTTG GGAGAAAAGG AATGAAATTG ATTTCTGTGC AGCTGATGAT 
GACTTTAGAA ATCAGTTCAA GGTTCCTCCC TTCCAGGATT GCATGTTAAG TTTCCTTGGA 
TTCTCTGATG ATGAGAGAGC TAACATGGAA GAAATGACAG AAATGCAAGG AGGACATTAT 
TTACCAGTTG GTGATGAAAG GTGTACGCAC TTAGTGGTTG AAGAAAGTAC AGTAAAAGAT 
CTTCCATTTG AGCCTTTAAA AAAGCTTTAT GTTGTAAAGC AAGAGTGGTT CTGGGGAAGC 
ATTCAGATGG ATGCCAGAGC TGGAGAGTCC ATGTATTTAT TTGAGAAGTC AGAGAGTCCT 
GGCCTCAAGA AGTCTGTGTC GCTACTTTCT CTGAATACTC CAAACAGCAA TCGTAAAAGA 
CGCCGACTAA AAGAAACTCT CGCACAGCTG ACTAGAGAAA CAGACATGTC GCCCTTTCCT 
CCTCGTAAGC GCCCATCGGC TGAACATTCG CTTTCAATTG GGTCCTTGCT GGATATTTCC 
AACACTCCAG AGTCGGGCAC TGCCAGCGGA GAAACACCAA AATCCTGTGC AAGACCTTCC 
AAAAACTCAA CGCCTCTTCC ACTAAAGCAG TCTGCAAGGT GGCAGGTTGC AAAGGAATTG 
TATCAGACAG AAAGTAACTA TGTTGATATT CTAACAACAA TCATTCAGTT ATTTCAGGTT 
CCGTTGGAGA AAGAAGGACA ACTTGGAGGA CCAATCCTTG CACAGGAAGA GATTAAGACC 
ATATTTGGCA GCATTCCAGA TATTCTTGAT GTGCATATTA AAATCAAGGA AGACCTGGAA 
GATCTTATGA TTAATTGGAC TGAGAACAAA AGTATTGGTG ATATCATTCT TAAATATTCA 
AAAGACTTGT TGAAAACATA CCCTCCATTT GTGAATTTCT TTGAAATGAG CAAGGAGACT 
ATAACTAGAT GTGAAAAACA GAAGCCAAGG TTTCATGCCT TCCTAAAGAT AAACCAAGCT 
AAACCTGAAT GTGGCCGCCA AAGCCTAGCT GAACTCCTTA TCCGTCCTGT TCAAAGGCTG 
CCTAGTGTTG CTCTACTACT AAATGATATT AAGAAGCATA CAGCAGAAGA GAACCCAGAT 
AAAGTAACTC TAGAGAGAGC TATTGAATCA TTAAAGGAAG TGATGACACA TATTAATGAA 
GACAAAAGAA AAACAGAGGC ACAAAAGCAG TTCTTTGACG TTGTCTATGA AGTTGACGGG 
TGTCCAGCCA ATCTCTTGTC CTCTCATCGA AGCTTAGTTC AGCGTTTGGA AACTGTAGCA 
CTTGGCGATG ACCTCTGTGA CAGGGGAGAG CAGGTCACAC TTTTTCTGTT CAATGATTGC 
CTAGAGATTG CAAGGAAACG GCATAAAGTC ATTGGTGCTT TCAAGAGTCC GCATGGCCAC 
ACAAGGCCTC CTGCGTCTCT CAAGCATGTT GTTCTCATGC CTCTCTCCCA GATCAAGAAA 
GTCCTAGACA TCAGGGAGAC AGAAGATTGC CATAAAGCTT TTGCCTTAGT TGTGCGGCCA 
CCTACAGAAC TTAACAACAA GCTACTCAGC TTCCAGATGA CAACTGAAGA ACCTCCTAAA 
GAAGATTGGT TGAAGATGTT GTGTCGGCAT GTGGCTAACA CTATTTGTAA AGCAGATGCA 
GAAAATCTCA TTTATACTGC TGATCCTGAT TCAGTTGAAG TAAATACTAA GGACATGGAC 
AGTACTTTAA GCAGAGCATC CAGGGCAATA AAGAAAACAT CAAAAAAGGT TACAAGAGCA 
TTTTCTTTCT CAAAAACACC AAAGAGAGCT CTTCGAAGGG CTTTAATGTC ACATAGTGCA 
ACAGAAGGAA GAAGTCCCAG CTCAGCTAAC GAGAGTTATA TAGGCAGCCG ACTGACTAGT 
ACGTCATCAT TAGCGGGTAT TCCTTCTCCT TCCTTGGTCA GTCTTCCCTC AATCTTTGAA 
AAAAGGAGTC ATACATTAAG TCGATCAACA ACCCACTTGA TATGA