HAL cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	HAL
Entrez Gene ID	104637049
Full Name	histidine ammonia-lyase
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010304398.1	XP_010302700.1	histidine ammonia-lyase

Pathways from BioSystems
Gene Ontology

The following HAL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa187546	XM_010304398.1 Latest version!	Balearica regulorum gibbericeps histidine ammonia-lyase (HAL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAL ( XM_010304398.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010302700.1 Balearica regulorum gibbericeps histidine ammonia-lyase (HAL), mRNA.

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CloneID	OBa187546
Clone ID Related Accession (Same CDS sequence)	XM_010304398.1
Accession Version	XM_010304398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	histidine ammonia-lyase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010760567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGAGAT ACACGGTGCA CGTCCGAGGA GAATGGCTGG CAGTGCCCTG CCTAAACCGC 
ACGAACACGA TCAGGTGGCT GGGAAAGGAA GCTGTGAGAC GGTACATGAA GAACAAACCC 
GACAACGGGG GATTTGCCTC GGTGGAAGAA GTCAAATTTT ACATTCGAAG GTGCAAGGGA 
CTTGGCTTGC TGGATCTTGA TGATACAGTG GAGGATGCTC TAGAGGACAA TGAATTTGTT 
GAAGTTGTTA TAGAAGGAGA TATAATGTCT CCAGATTTCA TACCATCTCA GCCAGAAGGA 
GTTCATTTAT ACAGCAAATA CCGAGAACCA GAACAGTATC TTTCTTTAGA TGGCAACAGC 
TTAACAACAG AGGACTTGGT CAATTTAGGA AAGGGGCTCT ACAAGATAAA GCTCACACCT 
GAAGCTGAAG CTAAAGTCAA ACAATCACGC GAAGTGATTG AAAGGATTGT AAACGAACAG 
ACGGTTGTTT ATGGAATCAC CACGGGGTTT GGGAAGTTTG CCAGGACTGT CATCCCAAAC 
AGTAAGCTGA GGGAGCTTCA AGTGAACTTG GTTCGTTCAC ATTCTGCAGG TGTGGGGAAA 
CCTTTGAGTC CAGAGAGATC TCGCATGCTG TTGGCACTGA GGATCAATGT CCTAGCAAAA 
GGATACAGTG GAATATCCCT AGAAACGCTC CAGCAAGTTA TTGAAGCATT TAATGCGTCC 
TGCCTTCCTT ATATCCCTGA GAAAGGGACA GTTGGAGCCA GCGGAGACTT GGCCCCCCTC 
TCTCATCTTG CTCTGGGATT AATTGGAGAA GGAAAGATGT GGTCCCCAAA GAGTGGCTGG 
GCTGATGCTA AATATGTCCT GGAAGCCCAT GGTCTGAGAC CAATTACCTT GAAACCAAAA 
GAGGGTTTGG CTCTCATCAA TGGGACACAA ATGATCACCT CACTGGGGTG CGAAGCGGTT 
GAAAGAGCCG GTGCTATTGC TAGACAAGCT GACATAATCG CTGCCCTTAC ACTTGAAGTC 
TTGAAGGGTA CAACAAGGGC CTTTGACACC GATATCCACG CAGTGCGCCC ACATCGAGGG 
CAGGCCGAAG TGGCATTTCG ATTCAGGTCC CTTCTAGACT CTGACCATCA TCCATCAGAA 
ATAGCAGAGA GCCATAGATT CTGTGACCGA GTTCAGGATG CATACACGAT GCGCTGCTGC 
CCTCAGGTCC ATGGAGTTGT AAATGATACA ATTGCTTTTG TGAAGGACAT CATGATGACT 
GAAATCAATA GTGCAACGGA CAACCCCATG GTGTTTGCTG AAAGAGCAGA GACCATTTCT 
GGAGGAAATT TTCATGGCGA ATATCCTGCA AAGGCTCTAG ACTATTTGGC AATTGGTGTG 
CACGAACTCG CTGCAATTAG TGAAAGAAGA ATTGAGAGGC TCTGCAACCC CTCGCTCAGC 
GAACTGCCTG CATTTTTAGT CACTGAAGGA GGTCTGAACT CTGGCTTCAT GATTGCACAC 
TGTACTGCAG CTGCCCTGGT TTCTGAGAAC AAAGCCTTGT GCCATCCCTC GTCTGTGGAT 
TCTCTCTCAA CTAGTGCTGC TACAGAGGAT CACGTGTCCA TGGGAGGATG GGCTGCAAGA 
AAAGCTTTGA GAGTTATTGA ACACGTGGAA CAAGTTCTGG CTATTGAGCT GCTCGCGGCT 
TGCCAGGGCA TTGAATTCCT ACGCCCTCTG AGAACAACAA CCCCATTGGA GAAGGTCTAT 
GACCTTGTGC GCTCTGTTGT GAGGCCTTGG ATGAAGGATC GCTTCATGGC CCCGGACATC 
GAAGCAGCTC ACAGGCTGCT CGTGGAGCAG AAGGTGTGGG AAGTGGCTAA CCCTTACATT 
GAGAAGTACA GGAGGGAACA CATCCCTGAA TCAAGACCCA TTTCACCGAC AGCTTTCTCC 
CTGGGATTGC TGGAAATGAG TTCAGGTGTT GGTCATGACC ACAGGCATCA GGATGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010302700.1
CDS	24..2006
Translation	MPRYTVHVRGEWLAVPCLNRTNTIRWLGKEAVRRYMKNKPDNGGFASVEEVKFYIRRCKGLGLLDLDDTVEDALEDNEFVEVVIEGDIMSPDFIPSQPEGVHLYSKYREPEQYLSLDGNSLTTEDLVNLGKGLYKIKLTPEAEAKVKQSREVIERIVNEQTVVYGITTGFGKFARTVIPNSKLRELQVNLVRSHSAGVGKPLSPERSRMLLALRINVLAKGYSGISLETLQQVIEAFNASCLPYIPEKGTVGASGDLAPLSHLALGLIGEGKMWSPKSGWADAKYVLEAHGLRPITLKPKEGLALINGTQMITSLGCEAVERAGAIARQADIIAALTLEVLKGTTRAFDTDIHAVRPHRGQAEVAFRFRSLLDSDHHPSEIAESHRFCDRVQDAYTMRCCPQVHGVVNDTIAFVKDIMMTEINSATDNPMVFAERAETISGGNFHGEYPAKALDYLAIGVHELAAISERRIERLCNPSLSELPAFLVTEGGLNSGFMIAHCTAAALVSENKALCHPSSVDSLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLRTTTPLEKVYDLVRSVVRPWMKDRFMAPDIEAAHRLLVEQKVWEVANPYIEKYRREHIPESRPISPTAFSLGLLEMSSGVGHDHRHQDEL

Target ORF information:

RefSeq Version	XM_010304398.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps histidine ammonia-lyase (HAL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010304398.1

ORF Insert Sequence:

ATGCCGAGAT ACACGGTGCA CGTCCGAGGA GAATGGCTGG CAGTGCCCTG CCTAAACCGC 
ACGAACACGA TCAGGTGGCT GGGAAAGGAA GCTGTGAGAC GGTACATGAA GAACAAACCC 
GACAACGGGG GATTTGCCTC GGTGGAAGAA GTCAAATTTT ACATTCGAAG GTGCAAGGGA 
CTTGGCTTGC TGGATCTTGA TGATACAGTG GAGGATGCTC TAGAGGACAA TGAATTTGTT 
GAAGTTGTTA TAGAAGGAGA TATAATGTCT CCAGATTTCA TACCATCTCA GCCAGAAGGA 
GTTCATTTAT ACAGCAAATA CCGAGAACCA GAACAGTATC TTTCTTTAGA TGGCAACAGC 
TTAACAACAG AGGACTTGGT CAATTTAGGA AAGGGGCTCT ACAAGATAAA GCTCACACCT 
GAAGCTGAAG CTAAAGTCAA ACAATCACGC GAAGTGATTG AAAGGATTGT AAACGAACAG 
ACGGTTGTTT ATGGAATCAC CACGGGGTTT GGGAAGTTTG CCAGGACTGT CATCCCAAAC 
AGTAAGCTGA GGGAGCTTCA AGTGAACTTG GTTCGTTCAC ATTCTGCAGG TGTGGGGAAA 
CCTTTGAGTC CAGAGAGATC TCGCATGCTG TTGGCACTGA GGATCAATGT CCTAGCAAAA 
GGATACAGTG GAATATCCCT AGAAACGCTC CAGCAAGTTA TTGAAGCATT TAATGCGTCC 
TGCCTTCCTT ATATCCCTGA GAAAGGGACA GTTGGAGCCA GCGGAGACTT GGCCCCCCTC 
TCTCATCTTG CTCTGGGATT AATTGGAGAA GGAAAGATGT GGTCCCCAAA GAGTGGCTGG 
GCTGATGCTA AATATGTCCT GGAAGCCCAT GGTCTGAGAC CAATTACCTT GAAACCAAAA 
GAGGGTTTGG CTCTCATCAA TGGGACACAA ATGATCACCT CACTGGGGTG CGAAGCGGTT 
GAAAGAGCCG GTGCTATTGC TAGACAAGCT GACATAATCG CTGCCCTTAC ACTTGAAGTC 
TTGAAGGGTA CAACAAGGGC CTTTGACACC GATATCCACG CAGTGCGCCC ACATCGAGGG 
CAGGCCGAAG TGGCATTTCG ATTCAGGTCC CTTCTAGACT CTGACCATCA TCCATCAGAA 
ATAGCAGAGA GCCATAGATT CTGTGACCGA GTTCAGGATG CATACACGAT GCGCTGCTGC 
CCTCAGGTCC ATGGAGTTGT AAATGATACA ATTGCTTTTG TGAAGGACAT CATGATGACT 
GAAATCAATA GTGCAACGGA CAACCCCATG GTGTTTGCTG AAAGAGCAGA GACCATTTCT 
GGAGGAAATT TTCATGGCGA ATATCCTGCA AAGGCTCTAG ACTATTTGGC AATTGGTGTG 
CACGAACTCG CTGCAATTAG TGAAAGAAGA ATTGAGAGGC TCTGCAACCC CTCGCTCAGC 
GAACTGCCTG CATTTTTAGT CACTGAAGGA GGTCTGAACT CTGGCTTCAT GATTGCACAC 
TGTACTGCAG CTGCCCTGGT TTCTGAGAAC AAAGCCTTGT GCCATCCCTC GTCTGTGGAT 
TCTCTCTCAA CTAGTGCTGC TACAGAGGAT CACGTGTCCA TGGGAGGATG GGCTGCAAGA 
AAAGCTTTGA GAGTTATTGA ACACGTGGAA CAAGTTCTGG CTATTGAGCT GCTCGCGGCT 
TGCCAGGGCA TTGAATTCCT ACGCCCTCTG AGAACAACAA CCCCATTGGA GAAGGTCTAT 
GACCTTGTGC GCTCTGTTGT GAGGCCTTGG ATGAAGGATC GCTTCATGGC CCCGGACATC 
GAAGCAGCTC ACAGGCTGCT CGTGGAGCAG AAGGTGTGGG AAGTGGCTAA CCCTTACATT 
GAGAAGTACA GGAGGGAACA CATCCCTGAA TCAAGACCCA TTTCACCGAC AGCTTTCTCC 
CTGGGATTGC TGGAAATGAG TTCAGGTGTT GGTCATGACC ACAGGCATCA GGATGAACTT 
TAA