API5 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	API5
Entrez Gene ID	104636619
Full Name	apoptosis inhibitor 5
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010303826.1	XP_010302128.1	LOW QUALITY PROTEIN: apoptosis inhibitor 5

Pathways from BioSystems
Gene Ontology

The following API5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the API5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa187298	XM_010303826.1 Latest version!	Balearica regulorum gibbericeps apoptosis inhibitor 5 (API5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

API5 ( XM_010303826.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010302128.1 Balearica regulorum gibbericeps apoptosis inhibitor 5 (API5), mRNA.

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CloneID	OBa187298
Clone ID Related Accession (Same CDS sequence)	XM_010303826.1
Accession Version	XM_010303826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	LOW QUALITY PROTEIN: apoptosis inhibitor 5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010758985.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAAGTC AATCCTTAGA GCTAAAGGAG ATTTTTACCG TCTCCATTAA CAGATCTTTT 
AAGAACTATG TTTTATCTCA GATTAAGTTA GCTGCTTTAA TAGATGTCAA AGATGCATTG 
GTGATCCATT CCAGTGGCAC AGTTGGTGGG AGAGGTAACC TCTACTTGAG AGACAATAAA 
CAAGCAGACA GAAGGCAGAA ACTCCTTGAA AAACTGCCCA AGTTCAGTGA AGGTGATCAT 
CATAAGGATG CGTACCAGGC AATCTTGGAT GGTGTGAAAG GAGGTGCCAA GGAGAAAAGA 
CTTGCAGCCC AGTTTATTCC TAAGTTCTTC AAGCATTTTC CTGAGCTAGC TGACTCGGCT 
ATCAATGCCC AGTTGGACCT CTGTGAGGAT GAAGACGTTT CTATCCGGCG ACAAGCAATC 
AAGGAATTGC CTCAGTTTGC CACTGGAGAT AATCTTCCTC GGGTAGCAGA CATACTGACC 
CAGCTTCTAC AGTCAGATGA TTCTGCAGAA TTCAATTTGG TGAACAATGC TTTGCTCAGT 
ATATTTAAGA TGGATGCTAA AGGGACTTTG GGAGGTTTAT TCAGTCAAAT TCTTCAGGGA 
GAGGATATTG TGAGAGAGCG AGCCATCAAG TTCCTCTCTA CGAAACTGAA AACCCTGCCT 
GAGGAGGTGA TGACAAAGGA GGTAGAAGAG TTCATATTGA CTGAATCAAA GAAGGTGCTG 
GAAGATGTGA CGGGCGAGGA ATTTGTTCTG TTCATGAAAA TATTGTCTGG ATTAAAGAGC 
TTACAGACAG TAAGTGGGAG GCAACAACTA GTAGAGCTGG TAGCCGAACA AGCTGACCTG 
GAACAAACAT TCAATCCATC TGATCCAGAT TGTGTGGACA GACTTCTACA GTGTACTCGC 
CAGGCAGTGC CACTCTTCTC AAAAAATGTT CATTCCACAA AATTTGTTAC TTACTTCTGT 
GAGCATGTTC TGCCAAACCT CAGTTCTTTG ACTACTCCAG TGGAGGGTCT TGATATCCAG 
TTAGAGGTTT TGAAGCTTCT TGCTGAAATG AGTTCATTTT GTGGTGATAT GGAAAAGCTT 
GAATCAAATT TGAAGAAACT GTTTGATAAA TTACTGGAGT ATATGCCCCT TCCTCCAGAA 
GAAGCAGAGA ACGGGGAAAA TGCTGGCAGT GAAGAGCCTA AGTTGCAGTT CAGTTATGTG 
GAGTGTTTAT TGTATAGCTT CCATCAGCTG GGTCGTAAAC TTCCAGACTT CCTCACAGCC 
AAGCTGAATG CAGAGAAATT GAAAGACTTT AAAATCAGGC TACAGTATTT TGCTCGAGGG 
TTGCAGGTGT ACATTCGACA ACTTCGTCTA GCCCTTCAAG GGAAAACAGG AGAAGCCTTG 
AAAACAGAAG AGAACAAGAT TAAAGTGGTT GCCTTGAAAA TAACCAATAA CATTAATGTT 
TTAATCAAGG ATCTCTTCCA CATTCCTCCT TCTTATAAAA GTACAGTTAC ACTGTCCTGG 
AAACCAGTAC AGAAGGCAGA TGCAAGTCAA AAAAGAGCAA GTGAAGATAC AACCTCAAGT 
TCACCCCCAA AGAAAGCTTC AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCAGGCAA 
ATATATAATC CTCCCAGTGG AAAATACAGC AGTAACCTGG GTAGCTTTTC TTACGAGCAA 
AGAGGTGGTT TCCGGGGTGG ACGAGGAAGA GGCTGGGGAG GACGTGGCAA TCGTAGCCGA 
GGAAGAATCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010302128.1
CDS	1..1755
Translation	MESQSLELKEIFTVSINRSFKNYVLSQIKLAALIDVKDALVIHSSGTVGGRGNLYLRDNKQADRRQKLLEKLPKFSEGDHHKDAYQAILDGVKGGAKEKRLAAQFIPKFFKHFPELADSAINAQLDLCEDEDVSIRRQAIKELPQFATGDNLPRVADILTQLLQSDDSAEFNLVNNALLSIFKMDAKGTLGGLFSQILQGEDIVRERAIKFLSTKLKTLPEEVMTKEVEEFILTESKKVLEDVTGEEFVLFMKILSGLKSLQTVSGRQQLVELVAEQADLEQTFNPSDPDCVDRLLQCTRQAVPLFSKNVHSTKFVTYFCEHVLPNLSSLTTPVEGLDIQLEVLKLLAEMSSFCGDMEKLESNLKKLFDKLLEYMPLPPEEAENGENAGSEEPKLQFSYVECLLYSFHQLGRKLPDFLTAKLNAEKLKDFKIRLQYFARGLQVYIRQLRLALQGKTGEALKTEENKIKVVALKITNNINVLIKDLFHIPPSYKSTVTLSWKPVQKADASQKRASEDTTSSSPPKKASnullnullnullnullnullnullnullnullnullnullnullRQIYNPPSGKYSSNLGSFSYEQRGGFRGGRGRGWGGRGNRSRGRIY

Target ORF information:

RefSeq Version	XM_010303826.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps apoptosis inhibitor 5 (API5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010303826.1

ORF Insert Sequence:

ATGGAAAGTC AATCCTTAGA GCTAAAGGAG ATTTTTACCG TCTCCATTAA CAGATCTTTT 
AAGAACTATG TTTTATCTCA GATTAAGTTA GCTGCTTTAA TAGATGTCAA AGATGCATTG 
GTGATCCATT CCAGTGGCAC AGTTGGTGGG AGAGGTAACC TCTACTTGAG AGACAATAAA 
CAAGCAGACA GAAGGCAGAA ACTCCTTGAA AAACTGCCCA AGTTCAGTGA AGGTGATCAT 
CATAAGGATG CGTACCAGGC AATCTTGGAT GGTGTGAAAG GAGGTGCCAA GGAGAAAAGA 
CTTGCAGCCC AGTTTATTCC TAAGTTCTTC AAGCATTTTC CTGAGCTAGC TGACTCGGCT 
ATCAATGCCC AGTTGGACCT CTGTGAGGAT GAAGACGTTT CTATCCGGCG ACAAGCAATC 
AAGGAATTGC CTCAGTTTGC CACTGGAGAT AATCTTCCTC GGGTAGCAGA CATACTGACC 
CAGCTTCTAC AGTCAGATGA TTCTGCAGAA TTCAATTTGG TGAACAATGC TTTGCTCAGT 
ATATTTAAGA TGGATGCTAA AGGGACTTTG GGAGGTTTAT TCAGTCAAAT TCTTCAGGGA 
GAGGATATTG TGAGAGAGCG AGCCATCAAG TTCCTCTCTA CGAAACTGAA AACCCTGCCT 
GAGGAGGTGA TGACAAAGGA GGTAGAAGAG TTCATATTGA CTGAATCAAA GAAGGTGCTG 
GAAGATGTGA CGGGCGAGGA ATTTGTTCTG TTCATGAAAA TATTGTCTGG ATTAAAGAGC 
TTACAGACAG TAAGTGGGAG GCAACAACTA GTAGAGCTGG TAGCCGAACA AGCTGACCTG 
GAACAAACAT TCAATCCATC TGATCCAGAT TGTGTGGACA GACTTCTACA GTGTACTCGC 
CAGGCAGTGC CACTCTTCTC AAAAAATGTT CATTCCACAA AATTTGTTAC TTACTTCTGT 
GAGCATGTTC TGCCAAACCT CAGTTCTTTG ACTACTCCAG TGGAGGGTCT TGATATCCAG 
TTAGAGGTTT TGAAGCTTCT TGCTGAAATG AGTTCATTTT GTGGTGATAT GGAAAAGCTT 
GAATCAAATT TGAAGAAACT GTTTGATAAA TTACTGGAGT ATATGCCCCT TCCTCCAGAA 
GAAGCAGAGA ACGGGGAAAA TGCTGGCAGT GAAGAGCCTA AGTTGCAGTT CAGTTATGTG 
GAGTGTTTAT TGTATAGCTT CCATCAGCTG GGTCGTAAAC TTCCAGACTT CCTCACAGCC 
AAGCTGAATG CAGAGAAATT GAAAGACTTT AAAATCAGGC TACAGTATTT TGCTCGAGGG 
TTGCAGGTGT ACATTCGACA ACTTCGTCTA GCCCTTCAAG GGAAAACAGG AGAAGCCTTG 
AAAACAGAAG AGAACAAGAT TAAAGTGGTT GCCTTGAAAA TAACCAATAA CATTAATGTT 
TTAATCAAGG ATCTCTTCCA CATTCCTCCT TCTTATAAAA GTACAGTTAC ACTGTCCTGG 
AAACCAGTAC AGAAGGCAGA TGCAAGTCAA AAAAGAGCAA GTGAAGATAC AACCTCAAGT 
TCACCCCCAA AGAAAGCTTC AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCAGGCAA 
ATATATAATC CTCCCAGTGG AAAATACAGC AGTAACCTGG GTAGCTTTTC TTACGAGCAA 
AGAGGTGGTT TCCGGGGTGG ACGAGGAAGA GGCTGGGGAG GACGTGGCAA TCGTAGCCGA 
GGAAGAATCT ACTGA