F751_5852 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_5852
Entrez Gene ID	23617243
Full Name	Phosphoethanolamine N-methyltransferase
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011401674.1	XP_011399976.1	Phosphoethanolamine N-methyltransferase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_5852 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_5852 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu63085	XM_011401674.1 Latest version!	Auxenochlorella protothecoides Phosphoethanolamine N-methyltransferase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_5852 ( XM_011401674.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011399976.1 Auxenochlorella protothecoides Phosphoethanolamine N-methyltransferase partial mRNA.

Price & Availability↑

CloneID	OAu63085
Clone ID Related Accession (Same CDS sequence)	XM_011401674.1
Accession Version	XM_011401674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	Phosphoethanolamine N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934305). COMPLETENESS: incomplete on both ends.

ATGGGAACTC CCCAGGAACG AGAAGCCCAG AAGAGCTACT GGGCGGAGCA CTCGAGCAAC 
GCCACGGTGG AGTCGATGAT GCTCGACAGC CAGGCTGCGG ATATTGACAA ACTGGAAAGG 
CCAGAGGTGC TCAAGCTCCT GGGGTCTGTG GACGGCCTGG ATGTTGTGGA ACTGGGTGCT 
GGCATCGGGC GATTCACCCG CCCCTTGGCG GAGGAGGCAC GCAGTGTGGT GGCACTGGAC 
TTCATGCCCA ACCTGATTGA GCAGAACAGG GTGGACAATG GTCACCTGGG CAACATCGAC 
TTCCGGTGCG GCGATGCCAC CGAGCTGGAC CTGCCCGCCG GCTGTGCCGA CCTGGTGTTC 
TCCAACTGGC TGCTCATGTA CCTTGCCGAC GACGAGGTGG CCAAGCTGGC TCACAACATG 
CTCACCTGGG TCAAGGTCGG GGGCACGGTC TTCTTCCGCG AGTCCTGCTT CAGGCAGTCT 
GGCGACAAGG CGCGCGGCAG CAACCCCACC CACTACCGCA ATCCCCGCGA GTACTTCAAA 
ATCTTCGACG AGTGCGAGGT GGTGCAGCCA GACGGCCGCG TCGCGGTGTT CGACCTGGTG 
TGCTGCAAGA GCGTGGACAC ATACGTGCGT GTCAAGCAGA ACCAGAACCA AGTCTGCTGG 
AAGTGGCGCA AGATCATTAA GCCCGCGCCC ACGGCCTTCC GCGTCGACCG GCACTTCCTG 
GACGGCCAGC GCTACACCGC GGGCAGCATC GCGCGCTACG AGTTTGTGTT TGGCCAGGGC 
TTCATCTCGC CCGGCGGCGA GCGGCTGACG CAGGAGCTAG CGGCGCGGCT GGGCCTCAAC 
CCCGACGCAC ACGTGCTGGA CGTGGGTTGC GGCGTCGGCG GCAGCGCCTT CCATTTGGCC 
CGCACCCTTG GCTGCTATGT GTACGGGATA GACTTGTCGG CCAACATGAT CCTGACGGCC 
CTGGAACGGG CCGCCGCCTC GGGCAACGGC GACAAGGCGA GCCGGCGGCT GGGTGGGATG 
GAGGGTGGGG TGTCCCTGGA GGTGAGCGAC GCCACGAGAC GCGCGCTGCC CGAGGCCTCC 
TTCGACGCGG TCTTCACACG TGACTCCCTG CTCCACATCC ACGACAAGCC GGCGCTGTTC 
TCCCGCCTGC TGCGCGCACT GCGCCCCGGC GGCCAGCTCC TGGTCACGGA CTACTGCCGC 
GGCGGCGGCC CGCCCTCCCC GGGCTTCTCC GCCTACATCG ACGACCGGGG GTACGACCTG 
GTGGCGGTGG CCGAGTACGC GGCCCTGCTC TCCGCCGCGG GGTTTGAGGT GCTGTCTGCG 
CGGGACGAAA CGCCGCGATT CGTCGCCACG CTGGGCGCCG AGCTGGAGCG CCTGCGGGAG 
CGCCGCGACG AGTTTGTGGC GCGCTTCTCG GAGCGGGAGC ACGCCGACAT GGTGGCCGGG 
TGGGAGGCCA AGCTTGAGCG CGCCCACGCA GGCGAGCACT GCTGGGGCGT CTTCCACGCA 
CGGCGGCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011399976.1
CDS	1..1512
Translation	MGTPQEREAQKSYWAEHSSNATVESMMLDSQAADIDKLERPEVLKLLGSVDGLDVVELGAGIGRFTRPLAEEARSVVALDFMPNLIEQNRVDNGHLGNIDFRCGDATELDLPAGCADLVFSNWLLMYLADDEVAKLAHNMLTWVKVGGTVFFRESCFRQSGDKARGSNPTHYRNPREYFKIFDECEVVQPDGRVAVFDLVCCKSVDTYVRVKQNQNQVCWKWRKIIKPAPTAFRVDRHFLDGQRYTAGSIARYEFVFGQGFISPGGERLTQELAARLGLNPDAHVLDVGCGVGGSAFHLARTLGCYVYGIDLSANMILTALERAAASGNGDKASRRLGGMEGGVSLEVSDATRRALPEASFDAVFTRDSLLHIHDKPALFSRLLRALRPGGQLLVTDYCRGGGPPSPGFSAYIDDRGYDLVAVAEYAALLSAAGFEVLSARDETPRFVATLGAELERLRERRDEFVARFSEREHADMVAGWEAKLERAHAGEHCWGVFHARRP

Target ORF information:

RefSeq Version	XM_011401674.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides Phosphoethanolamine N-methyltransferase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011401674.1

ORF Insert Sequence:

ATGGGAACTC CCCAGGAACG AGAAGCCCAG AAGAGCTACT GGGCGGAGCA CTCGAGCAAC 
GCCACGGTGG AGTCGATGAT GCTCGACAGC CAGGCTGCGG ATATTGACAA ACTGGAAAGG 
CCAGAGGTGC TCAAGCTCCT GGGGTCTGTG GACGGCCTGG ATGTTGTGGA ACTGGGTGCT 
GGCATCGGGC GATTCACCCG CCCCTTGGCG GAGGAGGCAC GCAGTGTGGT GGCACTGGAC 
TTCATGCCCA ACCTGATTGA GCAGAACAGG GTGGACAATG GTCACCTGGG CAACATCGAC 
TTCCGGTGCG GCGATGCCAC CGAGCTGGAC CTGCCCGCCG GCTGTGCCGA CCTGGTGTTC 
TCCAACTGGC TGCTCATGTA CCTTGCCGAC GACGAGGTGG CCAAGCTGGC TCACAACATG 
CTCACCTGGG TCAAGGTCGG GGGCACGGTC TTCTTCCGCG AGTCCTGCTT CAGGCAGTCT 
GGCGACAAGG CGCGCGGCAG CAACCCCACC CACTACCGCA ATCCCCGCGA GTACTTCAAA 
ATCTTCGACG AGTGCGAGGT GGTGCAGCCA GACGGCCGCG TCGCGGTGTT CGACCTGGTG 
TGCTGCAAGA GCGTGGACAC ATACGTGCGT GTCAAGCAGA ACCAGAACCA AGTCTGCTGG 
AAGTGGCGCA AGATCATTAA GCCCGCGCCC ACGGCCTTCC GCGTCGACCG GCACTTCCTG 
GACGGCCAGC GCTACACCGC GGGCAGCATC GCGCGCTACG AGTTTGTGTT TGGCCAGGGC 
TTCATCTCGC CCGGCGGCGA GCGGCTGACG CAGGAGCTAG CGGCGCGGCT GGGCCTCAAC 
CCCGACGCAC ACGTGCTGGA CGTGGGTTGC GGCGTCGGCG GCAGCGCCTT CCATTTGGCC 
CGCACCCTTG GCTGCTATGT GTACGGGATA GACTTGTCGG CCAACATGAT CCTGACGGCC 
CTGGAACGGG CCGCCGCCTC GGGCAACGGC GACAAGGCGA GCCGGCGGCT GGGTGGGATG 
GAGGGTGGGG TGTCCCTGGA GGTGAGCGAC GCCACGAGAC GCGCGCTGCC CGAGGCCTCC 
TTCGACGCGG TCTTCACACG TGACTCCCTG CTCCACATCC ACGACAAGCC GGCGCTGTTC 
TCCCGCCTGC TGCGCGCACT GCGCCCCGGC GGCCAGCTCC TGGTCACGGA CTACTGCCGC 
GGCGGCGGCC CGCCCTCCCC GGGCTTCTCC GCCTACATCG ACGACCGGGG GTACGACCTG 
GTGGCGGTGG CCGAGTACGC GGCCCTGCTC TCCGCCGCGG GGTTTGAGGT GCTGTCTGCG 
CGGGACGAAA CGCCGCGATT CGTCGCCACG CTGGGCGCCG AGCTGGAGCG CCTGCGGGAG 
CGCCGCGACG AGTTTGTGGC GCGCTTCTCG GAGCGGGAGC ACGCCGACAT GGTGGCCGGG 
TGGGAGGCCA AGCTTGAGCG CGCCCACGCA GGCGAGCACT GCTGGGGCGT CTTCCACGCA 
CGGCGGCCAT GA