F751_4651 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_4651
Entrez Gene ID	23616042
Full Name	PKHD-type hydroxylase TPA1
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011400752.1	XP_011399054.1	PKHD-type hydroxylase TPA1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_4651 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_4651 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu62177	XM_011400752.1 Latest version!	Auxenochlorella protothecoides PKHD-type hydroxylase TPA1 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_4651 ( XM_011400752.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011399054.1 Auxenochlorella protothecoides PKHD-type hydroxylase TPA1 partial mRNA.

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CloneID	OAu62177
Clone ID Related Accession (Same CDS sequence)	XM_011400752.1
Accession Version	XM_011400752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	PKHD-type hydroxylase TPA1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934258). COMPLETENESS: incomplete on both ends.

ATGACTTCTG ACTCGGAGCC AGTGCCCAAG AAGGCCAAGA CAGCAGGCCA TGCCGTCGAC 
ATCATCCGGC CCAGTGTCAC GGGGACGGAG TCGCGGGAGG CGCTGGCAGC AGCGTACAAG 
GCAGCCCAGC CATACCCACA CTGCGTCATC ACCGACATCT GCGAGCACGA TGCCCTGGCA 
GCGGTGCGCG AGGAGATCAT CTCCAACGTC CAGGCGACGT ACAAGGAGAC GGACCTGTTC 
AACATGTTCC AGACAGGCCA GTTGGGAATC GCTTGCCTTC ACCTTGCTCC AGCGCGCGAC 
CTCGCCAACC TGGACGGCCT GGATGCGGAA AGCGCGGCCA AGCTCCCCAC GGTGCGGGCG 
CTGCGCGACG CGCTCTACAG CCCCGACTTC CGCGCCTTTG TCTCCGCGGT GACGGGGTGC 
GGGGAGCTGT CGGACCGCAC CGACTGCGCC TGCAATGTGC ACGCCCGGGG CGGCCACCTG 
CTCTGCCACG ACGATGTCAT CGGCACTCGG GCGGTGTCCT TCATCATCTA CCTCACAGAC 
CCGGAGCGGC CCTGGAAGGC AGAGAATGGC GGCCTGCTGG AGCTCTACCC CCAGGACCCA 
GAGCTCAAGT CTCAGCCCGC CGTCTTCCCG AGCGCCAGCG TGCTCCCCCT CTGGAACACC 
ATGGCCATGT TTGCGGTGCA GCCAGGCGTC AGTTTCCACT CCATCCAGGA GAACTTTACT 
GTGGACCAGC CCCGGATGAG CATCCAGGGC TGGTTCCACC GGGACACTCC CCCCGAGGGC 
GCCGACTCGG CGACGCTGCG CCAGTTGCAG CTGCAGTCAG ACCCCCGGAC CCGGGACTTC 
CAGCTCATGC CCGGCGCCCA GCCTGGGGGC AGCGACGCAG CAGCCACTGC GGGGCCCCTG 
CCCGAGGCGG AGCTCGAGGT GCTGGCCCGC TACGTCAACC CCGTCTACCT GGCGGAGGCC 
ACCTGGCCCA AGGTGGCCGC TCGCTTCCGG GAGGAGGGGT CGGTGCAGCT GCAGGCCTTC 
CTCAAACCGG AGCTGGCGCG GGCGGCGACG GCAGCCTGCC AGGCGGCGGA TGAACGGGAC 
GGGCTGGGGG AGGGCCCACC GCCGGCATCC TACACGGTGG GCCTGGGCCC AAGCTGGGAG 
GCCGTGGGGC CGGCCTTCAA GCAGCGGTAC CTGCGGTACA GTGGCGAGCC AACCGGCACG 
CCGGAGGCCG GCTCGGACCC GGGACCCATA CTGGAGCGGG TGCGGGAGGA GCTCCTCACC 
AGCTCCGCTT TCGCGCACCT CGTCGAAAAG ATGACCACAC TGCGCCCGTG CACCGCGCGT 
GGCGAGGTGC GCCGTTTCCG GCGCGGCATG GACTACACGG TGGCCCACCA CGGTGTGCTG 
GCGGCGGCGC CCTGCCTGGA CGCCGTGCTG TGCATGCTGG GCTCCGGCCC CGGGGCCGGC 
GAGGCCTGGG AGGGGGGCGA GGTGGGCGGC TACGAGGCCT ACATGCTGGC CGACGATGAG 
GGGGAGGGCG GCGCCGGCGC CGCAGAGACG GCCGAGGTGT ACCGCTCGCC GGGGGATGCG 
GCGGGGGAGT CGGAGTCGGG CGTGCTCAAC ATCTCCCCTG CCGCCAATTG CCTCAGCCTG 
GTGCTGCGCG ATGAGGAGAC GGAGCTCCTC AAGTTTGTCA AGTATGTGTC GGCCGCCGCG 
CCGGGCAGTC GGTGGGACGT CGCCATGGAG TATCAGCTCG CCGACGATGA CGATGACGAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011399054.1
CDS	1..1746
Translation	MTSDSEPVPKKAKTAGHAVDIIRPSVTGTESREALAAAYKAAQPYPHCVITDICEHDALAAVREEIISNVQATYKETDLFNMFQTGQLGIACLHLAPARDLANLDGLDAESAAKLPTVRALRDALYSPDFRAFVSAVTGCGELSDRTDCACNVHARGGHLLCHDDVIGTRAVSFIIYLTDPERPWKAENGGLLELYPQDPELKSQPAVFPSASVLPLWNTMAMFAVQPGVSFHSIQENFTVDQPRMSIQGWFHRDTPPEGADSATLRQLQLQSDPRTRDFQLMPGAQPGGSDAAATAGPLPEAELEVLARYVNPVYLAEATWPKVAARFREEGSVQLQAFLKPELARAATAACQAADERDGLGEGPPPASYTVGLGPSWEAVGPAFKQRYLRYSGEPTGTPEAGSDPGPILERVREELLTSSAFAHLVEKMTTLRPCTARGEVRRFRRGMDYTVAHHGVLAAAPCLDAVLCMLGSGPGAGEAWEGGEVGGYEAYMLADDEGEGGAGAAETAEVYRSPGDAAGESESGVLNISPAANCLSLVLRDEETELLKFVKYVSAAAPGSRWDVAMEYQLADDDDDEE

Target ORF information:

RefSeq Version	XM_011400752.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides PKHD-type hydroxylase TPA1 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011400752.1

ORF Insert Sequence:

ATGACTTCTG ACTCGGAGCC AGTGCCCAAG AAGGCCAAGA CAGCAGGCCA TGCCGTCGAC 
ATCATCCGGC CCAGTGTCAC GGGGACGGAG TCGCGGGAGG CGCTGGCAGC AGCGTACAAG 
GCAGCCCAGC CATACCCACA CTGCGTCATC ACCGACATCT GCGAGCACGA TGCCCTGGCA 
GCGGTGCGCG AGGAGATCAT CTCCAACGTC CAGGCGACGT ACAAGGAGAC GGACCTGTTC 
AACATGTTCC AGACAGGCCA GTTGGGAATC GCTTGCCTTC ACCTTGCTCC AGCGCGCGAC 
CTCGCCAACC TGGACGGCCT GGATGCGGAA AGCGCGGCCA AGCTCCCCAC GGTGCGGGCG 
CTGCGCGACG CGCTCTACAG CCCCGACTTC CGCGCCTTTG TCTCCGCGGT GACGGGGTGC 
GGGGAGCTGT CGGACCGCAC CGACTGCGCC TGCAATGTGC ACGCCCGGGG CGGCCACCTG 
CTCTGCCACG ACGATGTCAT CGGCACTCGG GCGGTGTCCT TCATCATCTA CCTCACAGAC 
CCGGAGCGGC CCTGGAAGGC AGAGAATGGC GGCCTGCTGG AGCTCTACCC CCAGGACCCA 
GAGCTCAAGT CTCAGCCCGC CGTCTTCCCG AGCGCCAGCG TGCTCCCCCT CTGGAACACC 
ATGGCCATGT TTGCGGTGCA GCCAGGCGTC AGTTTCCACT CCATCCAGGA GAACTTTACT 
GTGGACCAGC CCCGGATGAG CATCCAGGGC TGGTTCCACC GGGACACTCC CCCCGAGGGC 
GCCGACTCGG CGACGCTGCG CCAGTTGCAG CTGCAGTCAG ACCCCCGGAC CCGGGACTTC 
CAGCTCATGC CCGGCGCCCA GCCTGGGGGC AGCGACGCAG CAGCCACTGC GGGGCCCCTG 
CCCGAGGCGG AGCTCGAGGT GCTGGCCCGC TACGTCAACC CCGTCTACCT GGCGGAGGCC 
ACCTGGCCCA AGGTGGCCGC TCGCTTCCGG GAGGAGGGGT CGGTGCAGCT GCAGGCCTTC 
CTCAAACCGG AGCTGGCGCG GGCGGCGACG GCAGCCTGCC AGGCGGCGGA TGAACGGGAC 
GGGCTGGGGG AGGGCCCACC GCCGGCATCC TACACGGTGG GCCTGGGCCC AAGCTGGGAG 
GCCGTGGGGC CGGCCTTCAA GCAGCGGTAC CTGCGGTACA GTGGCGAGCC AACCGGCACG 
CCGGAGGCCG GCTCGGACCC GGGACCCATA CTGGAGCGGG TGCGGGAGGA GCTCCTCACC 
AGCTCCGCTT TCGCGCACCT CGTCGAAAAG ATGACCACAC TGCGCCCGTG CACCGCGCGT 
GGCGAGGTGC GCCGTTTCCG GCGCGGCATG GACTACACGG TGGCCCACCA CGGTGTGCTG 
GCGGCGGCGC CCTGCCTGGA CGCCGTGCTG TGCATGCTGG GCTCCGGCCC CGGGGCCGGC 
GAGGCCTGGG AGGGGGGCGA GGTGGGCGGC TACGAGGCCT ACATGCTGGC CGACGATGAG 
GGGGAGGGCG GCGCCGGCGC CGCAGAGACG GCCGAGGTGT ACCGCTCGCC GGGGGATGCG 
GCGGGGGAGT CGGAGTCGGG CGTGCTCAAC ATCTCCCCTG CCGCCAATTG CCTCAGCCTG 
GTGCTGCGCG ATGAGGAGAC GGAGCTCCTC AAGTTTGTCA AGTATGTGTC GGCCGCCGCG 
CCGGGCAGTC GGTGGGACGT CGCCATGGAG TATCAGCTCG CCGACGATGA CGATGACGAG 
GAGTGA