F751_3685 cDNA ORF clone, Auxenochlorella protothecoides

Gene
Clones
gRNAs

Summary

Gene Symbol	F751_3685
Entrez Gene ID	23615076
Full Name	Ribosomal RNA large subunit methyltransferase I
Gene Type	protein-coding
Organism	Auxenochlorella protothecoides

mRNA and Protein(s)

mRNA	Protein	Name
XM_011403815.1	XP_011402117.1	Ribosomal RNA large subunit methyltransferase I

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Oil accumulation mechanisms of the oleaginous microalga Chlorella protothecoides revealed through its genome, transcriptomes, and proteomes.
Gao C, Wang Y, Shen Y, Yan D, He X, Dai J, Wu Q
BMC genomics15582(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following F751_3685 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F751_3685 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu65173	XM_011403815.1 Latest version!	Auxenochlorella protothecoides Ribosomal RNA large subunit methyltransferase I partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F751_3685 ( XM_011403815.1 ) cDNA ORF clone, Auxenochlorella protothecoides -> XP_011402117.1 Auxenochlorella protothecoides Ribosomal RNA large subunit methyltransferase I partial mRNA.

Price & Availability↑

CloneID	OAu65173
Clone ID Related Accession (Same CDS sequence)	XM_011403815.1
Accession Version	XM_011403815.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-23
Organism	Auxenochlorella protothecoides
Product	Ribosomal RNA large subunit methyltransferase I
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_011934477). COMPLETENESS: incomplete on both ends.

ATGCAGCATG CCACCGCATT GCTGCGCAGT GCACCATGCA TATCACTGAG GGCCTTTACG 
AGCGCCAAGA GACCGGGTAG CCGCGCCGGG CTGCACGTCC GTGCGGCGGT GGCCAGCAAG 
ACTGCCACGC CCCGCCTGGT GCTCAAGGGA GGCAAGGTCC ACCTCTTCTC CCGCCATCGC 
AACCCGGTTG TATACGGGGG CGCCGTGGAT AATATCAAGG GGCCGGCCCC CGCCCTTGGC 
GACGCTGTGC TGGTGTGCGA TGGAGCAGGC GCCGCGTTTG CATGGGGCTT CTACAACCCC 
CACTCCATGT ACCGCGTGCG GATCATGCAG ATGCTGTCGA GCCCTGAGCA AAAGGCGCCC 
AGCGACATCC AGGCCCTCCT TCGCGCCAAG ATTCAGGCCG CCTGGGAGCT GCGGCGGGCC 
CTGGACCTCC CCAGCCAGGA CACCGATGTC TACCGCCTCG TCAACAGTGA GGGCGATGGG 
CTGTCGGGCC TCATCATCGA CGTACTGGGC TCCCAGGTGG TGGTGGCCCC CTCGGCGGCC 
TGGTGCATGC GGTACCGCGC GGAGATCACC CGCGCGCTGA CCGAGGTCCT CCAGCAGCAG 
GGTACCGCAG GCGGGGCCGA GCCTGACATC GTGTGGCAGC CTGCCGTCAC CATGCTGACG 
CGCGAGGAGG GCTGGACCTT TGATGACCCC GGTGCGGCGG GGGTCCAGGG AGTGGCGATG 
AACGGGCCTC GCAGCAGGGC CGCCTCTGGC GAGGACTCTG GCGAGGAGGA GGAGGGCGAC 
GCAGGGGAGG ACGCAGAGAC GGACAGCGCG GCCTCTCCCG CCTCGCCCGC CGACCCGCCG 
CTGATCATCC GGGAGCATGG GCTGCGGTAC AGCCTGGACG CCGAGGGCCC CAACCAGAAG 
ACGGGCTTCT ACGCTGACCA GCGCGACAGC CGCCTCGTCC TGCGCCAGCT CAGCAAAGGA 
AAGCGCGTCC TGGACCTGTG TTGCTACACG GGCGGCTTCG CGTTGAACGC GGCCGCCGGC 
GGCGCGACCC ATGTGCTGGG CCTGGACAGC TCCCACCGCG CCGTGCACGC TGCGGCGGCC 
AACGCCGCGC TGAATGGGCT GGACCAGGTC CTGACCTTTG AGCGTCAGGA CATGAAGGCC 
TGGGGTCGCG GCAGCCAGCC GCCGCCGGGC GGCTGGGACA TGGTGGTCCT GGACCCGCCC 
AAGCTGGCGC CGGGGAAGAA GGCCATGGTG CGTGCCACGC GGCAGTACAC CGCCTACAAC 
GCGGCGGCCA TGCGCCTGGT GGCCCCAGGG GGGCTCCTCA TGACCTGCTC CTGCTCGGGG 
GCCATGACCC AAAGCGACGG CTTCCTGCCC ATGCTCCAGG ATGCCGCCCG CCTGGCGGGG 
CGCAGCCTGA CACTGCTGCG GTCGGCGGGG GCGGCGCCAG ACCACCCGCT CGACCCGGGC 
TACCCTGAGG GGCGGTACCT CACCAACGTC CTCGTGCGCG TGACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011402117.1
CDS	1..1488
Translation	MQHATALLRSAPCISLRAFTSAKRPGSRAGLHVRAAVASKTATPRLVLKGGKVHLFSRHRNPVVYGGAVDNIKGPAPALGDAVLVCDGAGAAFAWGFYNPHSMYRVRIMQMLSSPEQKAPSDIQALLRAKIQAAWELRRALDLPSQDTDVYRLVNSEGDGLSGLIIDVLGSQVVVAPSAAWCMRYRAEITRALTEVLQQQGTAGGAEPDIVWQPAVTMLTREEGWTFDDPGAAGVQGVAMNGPRSRAASGEDSGEEEEGDAGEDAETDSAASPASPADPPLIIREHGLRYSLDAEGPNQKTGFYADQRDSRLVLRQLSKGKRVLDLCCYTGGFALNAAAGGATHVLGLDSSHRAVHAAAANAALNGLDQVLTFERQDMKAWGRGSQPPPGGWDMVVLDPPKLAPGKKAMVRATRQYTAYNAAAMRLVAPGGLLMTCSCSGAMTQSDGFLPMLQDAARLAGRSLTLLRSAGAAPDHPLDPGYPEGRYLTNVLVRVT

Target ORF information:

RefSeq Version	XM_011403815.1
Organism	Auxenochlorella protothecoides
Definition	Auxenochlorella protothecoides Ribosomal RNA large subunit methyltransferase I partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011403815.1

ORF Insert Sequence:

ATGCAGCATG CCACCGCATT GCTGCGCAGT GCACCATGCA TATCACTGAG GGCCTTTACG 
AGCGCCAAGA GACCGGGTAG CCGCGCCGGG CTGCACGTCC GTGCGGCGGT GGCCAGCAAG 
ACTGCCACGC CCCGCCTGGT GCTCAAGGGA GGCAAGGTCC ACCTCTTCTC CCGCCATCGC 
AACCCGGTTG TATACGGGGG CGCCGTGGAT AATATCAAGG GGCCGGCCCC CGCCCTTGGC 
GACGCTGTGC TGGTGTGCGA TGGAGCAGGC GCCGCGTTTG CATGGGGCTT CTACAACCCC 
CACTCCATGT ACCGCGTGCG GATCATGCAG ATGCTGTCGA GCCCTGAGCA AAAGGCGCCC 
AGCGACATCC AGGCCCTCCT TCGCGCCAAG ATTCAGGCCG CCTGGGAGCT GCGGCGGGCC 
CTGGACCTCC CCAGCCAGGA CACCGATGTC TACCGCCTCG TCAACAGTGA GGGCGATGGG 
CTGTCGGGCC TCATCATCGA CGTACTGGGC TCCCAGGTGG TGGTGGCCCC CTCGGCGGCC 
TGGTGCATGC GGTACCGCGC GGAGATCACC CGCGCGCTGA CCGAGGTCCT CCAGCAGCAG 
GGTACCGCAG GCGGGGCCGA GCCTGACATC GTGTGGCAGC CTGCCGTCAC CATGCTGACG 
CGCGAGGAGG GCTGGACCTT TGATGACCCC GGTGCGGCGG GGGTCCAGGG AGTGGCGATG 
AACGGGCCTC GCAGCAGGGC CGCCTCTGGC GAGGACTCTG GCGAGGAGGA GGAGGGCGAC 
GCAGGGGAGG ACGCAGAGAC GGACAGCGCG GCCTCTCCCG CCTCGCCCGC CGACCCGCCG 
CTGATCATCC GGGAGCATGG GCTGCGGTAC AGCCTGGACG CCGAGGGCCC CAACCAGAAG 
ACGGGCTTCT ACGCTGACCA GCGCGACAGC CGCCTCGTCC TGCGCCAGCT CAGCAAAGGA 
AAGCGCGTCC TGGACCTGTG TTGCTACACG GGCGGCTTCG CGTTGAACGC GGCCGCCGGC 
GGCGCGACCC ATGTGCTGGG CCTGGACAGC TCCCACCGCG CCGTGCACGC TGCGGCGGCC 
AACGCCGCGC TGAATGGGCT GGACCAGGTC CTGACCTTTG AGCGTCAGGA CATGAAGGCC 
TGGGGTCGCG GCAGCCAGCC GCCGCCGGGC GGCTGGGACA TGGTGGTCCT GGACCCGCCC 
AAGCTGGCGC CGGGGAAGAA GGCCATGGTG CGTGCCACGC GGCAGTACAC CGCCTACAAC 
GCGGCGGCCA TGCGCCTGGT GGCCCCAGGG GGGCTCCTCA TGACCTGCTC CTGCTCGGGG 
GCCATGACCC AAAGCGACGG CTTCCTGCCC ATGCTCCAGG ATGCCGCCCG CCTGGCGGGG 
CGCAGCCTGA CACTGCTGCG GTCGGCGGGG GCGGCGCCAG ACCACCCGCT CGACCCGGGC 
TACCCTGAGG GGCGGTACCT CACCAACGTC CTCGTGCGCG TGACTTGA