LOC106522844 cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106522844
Entrez Gene ID	106522844
Full Name	uncharacterized LOC106522844
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014016036.1	XP_013871490.1	uncharacterized protein LOC106522844
XM_014016037.1	XP_013871491.1	uncharacterized protein LOC106522844

Pathways from BioSystems
Gene Ontology

The following LOC106522844 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106522844 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu15149	XM_014016036.1 Latest version!	Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAu15149	XM_014016037.1 Latest version!	Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106522844 ( XM_014016036.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013871490.1 Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAu15149
Clone ID Related Accession (Same CDS sequence)	XM_014016036.1 , XM_014016037.1
Accession Version	XM_014016036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	uncharacterized protein LOC106522844
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952539.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCT GGCTTTCTGG ACCATGGATG GGCGAGAAAA CGATGAATGG GCGGAGTCTA 
GCACACCTAA GCCAACGGGA TGCCCTGCGT CTCATCGCAG CCAGTCAGCG TCCAATCACC 
TTGCAGATCA AAGGCCAGAG GAGGCGGGGY ACTGATGCAA GCAGGGGGTC CTGGGAACCA 
CTTCCCCTCA ACCTGCAGCA CCTCAACCTA CCTCTTCCAC CTATGGGGGC GGGCATGAAC 
ACGTCTAGCC CCTCCTACCA AGACAGACAT TATTACAATC ATCTGTCTCT GCCGCAAGAC 
CACTGTGATG CGGGACGATA CAGCTACCTG TCGAGTTCTC CGCAGGACAC AGTGGACATC 
AACCATCAAG ATCTAGAACT TGCTGGCCGT CGAACAAAGA GACGGAACTG CCTGACGGGA 
TGTTGCAACG CTGACTTAGA TGAACCGACC ACCTGCAACA GCCAGACCGA TGATGAGGAC 
TTCATACTGG AGAAGCCACT GGGCTTCATG CCTCTTCACC ATGAACTGGA TAGTGGACTG 
GGCTGGACCG ACGGCTCCCT TCACCAGGGA GACTTGTCAG GGCTGGAGAC YGAAGAGGGA 
GGTCTGGAGG ACTGTCACCC ACATGGGAAC CACGCGGCGG CAGGCTGCGG GGGGTTCGGC 
GGCGGGGGCT CTCCATCCTC CGAGTCCTTC ATCTCCTCGG AGCTCAGCGA CTCGGGCTTC 
TACAGCGTGA GCACCGGGGA GTTCAGGCAC TTCCAGAGGC TGCTGGAGAA GAGGATGCGT 
CTGTACAACG CAAGGATGCA ACATCAGGGC GAACCGTGCG AGAGACGGGA GAGGAGGGAC 
AGCTGCCCCA AGAGCCACCG CGAGCTGCTG GAGGCCATAC CGGAGGCACT GACGATGCAG 
CCTCAGTGTC AGCACCTGCA GCACGGCATG GAGGACGGAG CTGGACCCAT GATGGATGTC 
CCACCTCGTG GACTTTTCAG GGTTTCATCA GTCCAGTTCC ATAAAACTGA CAGACCCTGT 
CTGAACCGGC ACAGCTCCAG CAGCGGAGCG CTCTTCAACC CCAACAATGC CGCCGTTTCA 
CGAATGACGG CACCAGTCCT CTCCACCTGC AGCACCCCCT CCAGCCACCG ACGTCCACAC 
ATACCCATGC AGCAGCAGTT CCACCATCAG GGCTCCAGCT CGGTGGGAGT GCTGAGGAGG 
AGCCGGACCA TGCACCATCG CCCTCCTCCG CAGGARTACC GCCGCCGGGC AAGCCACCCA 
GCGTCCCCTT CTTATTGTGC AGCGACGATG CACTACTGTG GAGGGGGCGC GCCACGAAGC 
GCCCTGCCAC CTGGACTGCC AGAGGAAGGC GAGCTAGAGG CAGGCGTGAT GGCTGCCCGT 
CCAGCAGGCA TGGCTCTGTC ACCCCAGCAA CATCCAGCTG CTCTGGTGCA CATGAGAGGT 
GCCAGCAACA ACAACGAGCG GTGCTACGAC AACAATGGAC AGATTGCTCA CAGTGACTGG 
TGGCACTCCC AAGAACGAGG CCTGATGCCT GAACCCATGA TGACAGAACG GGAAAGGGAG 
CTGGAAAGGG AGCTGGAACA GAAAAGACAA ATGCAAAAGG AGAAAGAGCT GGAGAGGGAG 
AGGGAGGCAA AGATCCAGGA GGAGAAAGAA CGAGAGAGGC AGCAGGAGCT SGAGAGGAGC 
CGAGCCGGCG AGCAGCAGCC TCTTCTGAGT CACGTCCACC CTTCGCAGGC TGGAGACGTC 
AACGACACGT GGCCCAAGCC GGCCAGCCGT CAGTCGCACG GTGGTGGGGG AGGCAGAGGT 
CTTTACAGCA CCCTGGAGGG CCACATGGGG GCAGGAACCG CGGCCGGATG CGACGCCAAC 
AAGGGGCAAA TGAAYGGGAA TCCGAGCCCC AACCCCAGCT GTGGTCTACA GCCGAAGCTC 
AACCCGAGCT ACAAGCCCAG CGCGGCGTCA CGGATCACCA GGAGCCAGCT GCTCAGAGAC 
CGGGCGTCTC AGCTGGCCGA CGAGCGCAGC GGGATGAGCA CCGACGAGGA GACCAGCACC 
GACATGCTGC GGGGTCGATA CTGGAGCCGG ACGGAGAGGA AGGAACACTT CCTGTTGGCT 
CGCGAGCAAA AGCAGCAGCA GCTGCTGGCC AGAGGCTTGA CTGTACGAGA CAGCATTGGC 
AGGGGAGGAG CCTCTTTCGG GGACGGCGCC GTGCTGGAGA GCCGAGGAGT TGGACCGTTT 
GCGGAGGGAA GGTGCAACAC GGTCCTGGAA CTGAGTCAAA GGAAGCTGAG TCGTCTGAGG 
AACAGAAAGC TGTTGGATGA CTGGACGACA GTGGAGGAGC TGCTGACTCA TGGGACCAGG 
CTGGACAACC AGGAGGACAT GCTGTGTCCA AGCTCCCTGC TCACAGTTAC CACTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013871490.1
CDS	377..2776
Translation	MGCWLSGPWMGEKTMNGRSLAHLSQRDALRLIAASQRPITLQIKGQRRRnullTDASRGSWEPLPLNLQHLNLPLPPMGAGMNTSSPSYQDRHYYNHLSLPQDHCDAGRYSYLSSSPQDTVDINHQDLELAGRRTKRRNCLTGCCNADLDEPTTCNSQTDDEDFILEKPLGFMPLHHELDSGLGWTDGSLHQGDLSGLEnullEEGGLEDCHPHGNHAAAGCGGFGGGGSPSSESFISSELSDSGFYSVSTGEFRHFQRLLEKRMRLYNARMQHQGEPCERRERRDSCPKSHRELLEAIPEALTMQPQCQHLQHGMEDGAGPMMDVPPRGLFRVSSVQFHKTDRPCLNRHSSSSGALFNPNNAAVSRMTAPVLSTCSTPSSHRRPHIPMQQQFHHQGSSSVGVLRRSRTMHHRPPPQnullYRRRASHPASPSYCAATMHYCGGGAPRSALPPGLPEEGELEAGVMAARPAGMALSPQQHPAALVHMRGASNNNERCYDNNGQIAHSDWWHSQERGLMPEPMMTERERELERELEQKRQMQKEKELEREREAKIQEEKERERQQEnullERSRAGEQQPLLSHVHPSQAGDVNDTWPKPASRQSHGGGGGRGLYSTLEGHMGAGTAAGCDANKGQMnullGNPSPNPSCGLQPKLNPSYKPSAASRITRSQLLRDRASQLADERSGMSTDEETSTDMLRGRYWSRTERKEHFLLAREQKQQQLLARGLTVRDSIGRGGASFGDGAVLESRGVGPFAEGRCNTVLELSQRKLSRLRNRKLLDDWTTVEELLTHGTRLDNQEDMLCPSSLLTVTTV

Target ORF information:

RefSeq Version	XM_014016036.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014016036.1

ORF Insert Sequence:

ATGGGCTGCT GGCTTTCTGG ACCATGGATG GGCGAGAAAA CGATGAATGG GCGGAGTCTA 
GCACACCTAA GCCAACGGGA TGCCCTGCGT CTCATCGCAG CCAGTCAGCG TCCAATCACC 
TTGCAGATCA AAGGCCAGAG GAGGCGGGGY ACTGATGCAA GCAGGGGGTC CTGGGAACCA 
CTTCCCCTCA ACCTGCAGCA CCTCAACCTA CCTCTTCCAC CTATGGGGGC GGGCATGAAC 
ACGTCTAGCC CCTCCTACCA AGACAGACAT TATTACAATC ATCTGTCTCT GCCGCAAGAC 
CACTGTGATG CGGGACGATA CAGCTACCTG TCGAGTTCTC CGCAGGACAC AGTGGACATC 
AACCATCAAG ATCTAGAACT TGCTGGCCGT CGAACAAAGA GACGGAACTG CCTGACGGGA 
TGTTGCAACG CTGACTTAGA TGAACCGACC ACCTGCAACA GCCAGACCGA TGATGAGGAC 
TTCATACTGG AGAAGCCACT GGGCTTCATG CCTCTTCACC ATGAACTGGA TAGTGGACTG 
GGCTGGACCG ACGGCTCCCT TCACCAGGGA GACTTGTCAG GGCTGGAGAC YGAAGAGGGA 
GGTCTGGAGG ACTGTCACCC ACATGGGAAC CACGCGGCGG CAGGCTGCGG GGGGTTCGGC 
GGCGGGGGCT CTCCATCCTC CGAGTCCTTC ATCTCCTCGG AGCTCAGCGA CTCGGGCTTC 
TACAGCGTGA GCACCGGGGA GTTCAGGCAC TTCCAGAGGC TGCTGGAGAA GAGGATGCGT 
CTGTACAACG CAAGGATGCA ACATCAGGGC GAACCGTGCG AGAGACGGGA GAGGAGGGAC 
AGCTGCCCCA AGAGCCACCG CGAGCTGCTG GAGGCCATAC CGGAGGCACT GACGATGCAG 
CCTCAGTGTC AGCACCTGCA GCACGGCATG GAGGACGGAG CTGGACCCAT GATGGATGTC 
CCACCTCGTG GACTTTTCAG GGTTTCATCA GTCCAGTTCC ATAAAACTGA CAGACCCTGT 
CTGAACCGGC ACAGCTCCAG CAGCGGAGCG CTCTTCAACC CCAACAATGC CGCCGTTTCA 
CGAATGACGG CACCAGTCCT CTCCACCTGC AGCACCCCCT CCAGCCACCG ACGTCCACAC 
ATACCCATGC AGCAGCAGTT CCACCATCAG GGCTCCAGCT CGGTGGGAGT GCTGAGGAGG 
AGCCGGACCA TGCACCATCG CCCTCCTCCG CAGGARTACC GCCGCCGGGC AAGCCACCCA 
GCGTCCCCTT CTTATTGTGC AGCGACGATG CACTACTGTG GAGGGGGCGC GCCACGAAGC 
GCCCTGCCAC CTGGACTGCC AGAGGAAGGC GAGCTAGAGG CAGGCGTGAT GGCTGCCCGT 
CCAGCAGGCA TGGCTCTGTC ACCCCAGCAA CATCCAGCTG CTCTGGTGCA CATGAGAGGT 
GCCAGCAACA ACAACGAGCG GTGCTACGAC AACAATGGAC AGATTGCTCA CAGTGACTGG 
TGGCACTCCC AAGAACGAGG CCTGATGCCT GAACCCATGA TGACAGAACG GGAAAGGGAG 
CTGGAAAGGG AGCTGGAACA GAAAAGACAA ATGCAAAAGG AGAAAGAGCT GGAGAGGGAG 
AGGGAGGCAA AGATCCAGGA GGAGAAAGAA CGAGAGAGGC AGCAGGAGCT SGAGAGGAGC 
CGAGCCGGCG AGCAGCAGCC TCTTCTGAGT CACGTCCACC CTTCGCAGGC TGGAGACGTC 
AACGACACGT GGCCCAAGCC GGCCAGCCGT CAGTCGCACG GTGGTGGGGG AGGCAGAGGT 
CTTTACAGCA CCCTGGAGGG CCACATGGGG GCAGGAACCG CGGCCGGATG CGACGCCAAC 
AAGGGGCAAA TGAAYGGGAA TCCGAGCCCC AACCCCAGCT GTGGTCTACA GCCGAAGCTC 
AACCCGAGCT ACAAGCCCAG CGCGGCGTCA CGGATCACCA GGAGCCAGCT GCTCAGAGAC 
CGGGCGTCTC AGCTGGCCGA CGAGCGCAGC GGGATGAGCA CCGACGAGGA GACCAGCACC 
GACATGCTGC GGGGTCGATA CTGGAGCCGG ACGGAGAGGA AGGAACACTT CCTGTTGGCT 
CGCGAGCAAA AGCAGCAGCA GCTGCTGGCC AGAGGCTTGA CTGTACGAGA CAGCATTGGC 
AGGGGAGGAG CCTCTTTCGG GGACGGCGCC GTGCTGGAGA GCCGAGGAGT TGGACCGTTT 
GCGGAGGGAA GGTGCAACAC GGTCCTGGAA CTGAGTCAAA GGAAGCTGAG TCGTCTGAGG 
AACAGAAAGC TGTTGGATGA CTGGACGACA GTGGAGGAGC TGCTGACTCA TGGGACCAGG 
CTGGACAACC AGGAGGACAT GCTGTGTCCA AGCTCCCTGC TCACAGTTAC CACTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106522844 ( XM_014016037.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013871491.1 Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAu15149
Clone ID Related Accession (Same CDS sequence)	XM_014016036.1 , XM_014016037.1
Accession Version	XM_014016037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2400bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	uncharacterized protein LOC106522844
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952539.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCT GGCTTTCTGG ACCATGGATG GGCGAGAAAA CGATGAATGG GCGGAGTCTA 
GCACACCTAA GCCAACGGGA TGCCCTGCGT CTCATCGCAG CCAGTCAGCG TCCAATCACC 
TTGCAGATCA AAGGCCAGAG GAGGCGGGGY ACTGATGCAA GCAGGGGGTC CTGGGAACCA 
CTTCCCCTCA ACCTGCAGCA CCTCAACCTA CCTCTTCCAC CTATGGGGGC GGGCATGAAC 
ACGTCTAGCC CCTCCTACCA AGACAGACAT TATTACAATC ATCTGTCTCT GCCGCAAGAC 
CACTGTGATG CGGGACGATA CAGCTACCTG TCGAGTTCTC CGCAGGACAC AGTGGACATC 
AACCATCAAG ATCTAGAACT TGCTGGCCGT CGAACAAAGA GACGGAACTG CCTGACGGGA 
TGTTGCAACG CTGACTTAGA TGAACCGACC ACCTGCAACA GCCAGACCGA TGATGAGGAC 
TTCATACTGG AGAAGCCACT GGGCTTCATG CCTCTTCACC ATGAACTGGA TAGTGGACTG 
GGCTGGACCG ACGGCTCCCT TCACCAGGGA GACTTGTCAG GGCTGGAGAC YGAAGAGGGA 
GGTCTGGAGG ACTGTCACCC ACATGGGAAC CACGCGGCGG CAGGCTGCGG GGGGTTCGGC 
GGCGGGGGCT CTCCATCCTC CGAGTCCTTC ATCTCCTCGG AGCTCAGCGA CTCGGGCTTC 
TACAGCGTGA GCACCGGGGA GTTCAGGCAC TTCCAGAGGC TGCTGGAGAA GAGGATGCGT 
CTGTACAACG CAAGGATGCA ACATCAGGGC GAACCGTGCG AGAGACGGGA GAGGAGGGAC 
AGCTGCCCCA AGAGCCACCG CGAGCTGCTG GAGGCCATAC CGGAGGCACT GACGATGCAG 
CCTCAGTGTC AGCACCTGCA GCACGGCATG GAGGACGGAG CTGGACCCAT GATGGATGTC 
CCACCTCGTG GACTTTTCAG GGTTTCATCA GTCCAGTTCC ATAAAACTGA CAGACCCTGT 
CTGAACCGGC ACAGCTCCAG CAGCGGAGCG CTCTTCAACC CCAACAATGC CGCCGTTTCA 
CGAATGACGG CACCAGTCCT CTCCACCTGC AGCACCCCCT CCAGCCACCG ACGTCCACAC 
ATACCCATGC AGCAGCAGTT CCACCATCAG GGCTCCAGCT CGGTGGGAGT GCTGAGGAGG 
AGCCGGACCA TGCACCATCG CCCTCCTCCG CAGGARTACC GCCGCCGGGC AAGCCACCCA 
GCGTCCCCTT CTTATTGTGC AGCGACGATG CACTACTGTG GAGGGGGCGC GCCACGAAGC 
GCCCTGCCAC CTGGACTGCC AGAGGAAGGC GAGCTAGAGG CAGGCGTGAT GGCTGCCCGT 
CCAGCAGGCA TGGCTCTGTC ACCCCAGCAA CATCCAGCTG CTCTGGTGCA CATGAGAGGT 
GCCAGCAACA ACAACGAGCG GTGCTACGAC AACAATGGAC AGATTGCTCA CAGTGACTGG 
TGGCACTCCC AAGAACGAGG CCTGATGCCT GAACCCATGA TGACAGAACG GGAAAGGGAG 
CTGGAAAGGG AGCTGGAACA GAAAAGACAA ATGCAAAAGG AGAAAGAGCT GGAGAGGGAG 
AGGGAGGCAA AGATCCAGGA GGAGAAAGAA CGAGAGAGGC AGCAGGAGCT SGAGAGGAGC 
CGAGCCGGCG AGCAGCAGCC TCTTCTGAGT CACGTCCACC CTTCGCAGGC TGGAGACGTC 
AACGACACGT GGCCCAAGCC GGCCAGCCGT CAGTCGCACG GTGGTGGGGG AGGCAGAGGT 
CTTTACAGCA CCCTGGAGGG CCACATGGGG GCAGGAACCG CGGCCGGATG CGACGCCAAC 
AAGGGGCAAA TGAAYGGGAA TCCGAGCCCC AACCCCAGCT GTGGTCTACA GCCGAAGCTC 
AACCCGAGCT ACAAGCCCAG CGCGGCGTCA CGGATCACCA GGAGCCAGCT GCTCAGAGAC 
CGGGCGTCTC AGCTGGCCGA CGAGCGCAGC GGGATGAGCA CCGACGAGGA GACCAGCACC 
GACATGCTGC GGGGTCGATA CTGGAGCCGG ACGGAGAGGA AGGAACACTT CCTGTTGGCT 
CGCGAGCAAA AGCAGCAGCA GCTGCTGGCC AGAGGCTTGA CTGTACGAGA CAGCATTGGC 
AGGGGAGGAG CCTCTTTCGG GGACGGCGCC GTGCTGGAGA GCCGAGGAGT TGGACCGTTT 
GCGGAGGGAA GGTGCAACAC GGTCCTGGAA CTGAGTCAAA GGAAGCTGAG TCGTCTGAGG 
AACAGAAAGC TGTTGGATGA CTGGACGACA GTGGAGGAGC TGCTGACTCA TGGGACCAGG 
CTGGACAACC AGGAGGACAT GCTGTGTCCA AGCTCCCTGC TCACAGTTAC CACTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013871491.1
CDS	425..2824
Translation	MGCWLSGPWMGEKTMNGRSLAHLSQRDALRLIAASQRPITLQIKGQRRRnullTDASRGSWEPLPLNLQHLNLPLPPMGAGMNTSSPSYQDRHYYNHLSLPQDHCDAGRYSYLSSSPQDTVDINHQDLELAGRRTKRRNCLTGCCNADLDEPTTCNSQTDDEDFILEKPLGFMPLHHELDSGLGWTDGSLHQGDLSGLEnullEEGGLEDCHPHGNHAAAGCGGFGGGGSPSSESFISSELSDSGFYSVSTGEFRHFQRLLEKRMRLYNARMQHQGEPCERRERRDSCPKSHRELLEAIPEALTMQPQCQHLQHGMEDGAGPMMDVPPRGLFRVSSVQFHKTDRPCLNRHSSSSGALFNPNNAAVSRMTAPVLSTCSTPSSHRRPHIPMQQQFHHQGSSSVGVLRRSRTMHHRPPPQnullYRRRASHPASPSYCAATMHYCGGGAPRSALPPGLPEEGELEAGVMAARPAGMALSPQQHPAALVHMRGASNNNERCYDNNGQIAHSDWWHSQERGLMPEPMMTERERELERELEQKRQMQKEKELEREREAKIQEEKERERQQEnullERSRAGEQQPLLSHVHPSQAGDVNDTWPKPASRQSHGGGGGRGLYSTLEGHMGAGTAAGCDANKGQMnullGNPSPNPSCGLQPKLNPSYKPSAASRITRSQLLRDRASQLADERSGMSTDEETSTDMLRGRYWSRTERKEHFLLAREQKQQQLLARGLTVRDSIGRGGASFGDGAVLESRGVGPFAEGRCNTVLELSQRKLSRLRNRKLLDDWTTVEELLTHGTRLDNQEDMLCPSSLLTVTTV

Target ORF information:

RefSeq Version	XM_014016037.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus uncharacterized LOC106522844 (LOC106522844), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014016037.1

ORF Insert Sequence:

ATGGGCTGCT GGCTTTCTGG ACCATGGATG GGCGAGAAAA CGATGAATGG GCGGAGTCTA 
GCACACCTAA GCCAACGGGA TGCCCTGCGT CTCATCGCAG CCAGTCAGCG TCCAATCACC 
TTGCAGATCA AAGGCCAGAG GAGGCGGGGY ACTGATGCAA GCAGGGGGTC CTGGGAACCA 
CTTCCCCTCA ACCTGCAGCA CCTCAACCTA CCTCTTCCAC CTATGGGGGC GGGCATGAAC 
ACGTCTAGCC CCTCCTACCA AGACAGACAT TATTACAATC ATCTGTCTCT GCCGCAAGAC 
CACTGTGATG CGGGACGATA CAGCTACCTG TCGAGTTCTC CGCAGGACAC AGTGGACATC 
AACCATCAAG ATCTAGAACT TGCTGGCCGT CGAACAAAGA GACGGAACTG CCTGACGGGA 
TGTTGCAACG CTGACTTAGA TGAACCGACC ACCTGCAACA GCCAGACCGA TGATGAGGAC 
TTCATACTGG AGAAGCCACT GGGCTTCATG CCTCTTCACC ATGAACTGGA TAGTGGACTG 
GGCTGGACCG ACGGCTCCCT TCACCAGGGA GACTTGTCAG GGCTGGAGAC YGAAGAGGGA 
GGTCTGGAGG ACTGTCACCC ACATGGGAAC CACGCGGCGG CAGGCTGCGG GGGGTTCGGC 
GGCGGGGGCT CTCCATCCTC CGAGTCCTTC ATCTCCTCGG AGCTCAGCGA CTCGGGCTTC 
TACAGCGTGA GCACCGGGGA GTTCAGGCAC TTCCAGAGGC TGCTGGAGAA GAGGATGCGT 
CTGTACAACG CAAGGATGCA ACATCAGGGC GAACCGTGCG AGAGACGGGA GAGGAGGGAC 
AGCTGCCCCA AGAGCCACCG CGAGCTGCTG GAGGCCATAC CGGAGGCACT GACGATGCAG 
CCTCAGTGTC AGCACCTGCA GCACGGCATG GAGGACGGAG CTGGACCCAT GATGGATGTC 
CCACCTCGTG GACTTTTCAG GGTTTCATCA GTCCAGTTCC ATAAAACTGA CAGACCCTGT 
CTGAACCGGC ACAGCTCCAG CAGCGGAGCG CTCTTCAACC CCAACAATGC CGCCGTTTCA 
CGAATGACGG CACCAGTCCT CTCCACCTGC AGCACCCCCT CCAGCCACCG ACGTCCACAC 
ATACCCATGC AGCAGCAGTT CCACCATCAG GGCTCCAGCT CGGTGGGAGT GCTGAGGAGG 
AGCCGGACCA TGCACCATCG CCCTCCTCCG CAGGARTACC GCCGCCGGGC AAGCCACCCA 
GCGTCCCCTT CTTATTGTGC AGCGACGATG CACTACTGTG GAGGGGGCGC GCCACGAAGC 
GCCCTGCCAC CTGGACTGCC AGAGGAAGGC GAGCTAGAGG CAGGCGTGAT GGCTGCCCGT 
CCAGCAGGCA TGGCTCTGTC ACCCCAGCAA CATCCAGCTG CTCTGGTGCA CATGAGAGGT 
GCCAGCAACA ACAACGAGCG GTGCTACGAC AACAATGGAC AGATTGCTCA CAGTGACTGG 
TGGCACTCCC AAGAACGAGG CCTGATGCCT GAACCCATGA TGACAGAACG GGAAAGGGAG 
CTGGAAAGGG AGCTGGAACA GAAAAGACAA ATGCAAAAGG AGAAAGAGCT GGAGAGGGAG 
AGGGAGGCAA AGATCCAGGA GGAGAAAGAA CGAGAGAGGC AGCAGGAGCT SGAGAGGAGC 
CGAGCCGGCG AGCAGCAGCC TCTTCTGAGT CACGTCCACC CTTCGCAGGC TGGAGACGTC 
AACGACACGT GGCCCAAGCC GGCCAGCCGT CAGTCGCACG GTGGTGGGGG AGGCAGAGGT 
CTTTACAGCA CCCTGGAGGG CCACATGGGG GCAGGAACCG CGGCCGGATG CGACGCCAAC 
AAGGGGCAAA TGAAYGGGAA TCCGAGCCCC AACCCCAGCT GTGGTCTACA GCCGAAGCTC 
AACCCGAGCT ACAAGCCCAG CGCGGCGTCA CGGATCACCA GGAGCCAGCT GCTCAGAGAC 
CGGGCGTCTC AGCTGGCCGA CGAGCGCAGC GGGATGAGCA CCGACGAGGA GACCAGCACC 
GACATGCTGC GGGGTCGATA CTGGAGCCGG ACGGAGAGGA AGGAACACTT CCTGTTGGCT 
CGCGAGCAAA AGCAGCAGCA GCTGCTGGCC AGAGGCTTGA CTGTACGAGA CAGCATTGGC 
AGGGGAGGAG CCTCTTTCGG GGACGGCGCC GTGCTGGAGA GCCGAGGAGT TGGACCGTTT 
GCGGAGGGAA GGTGCAACAC GGTCCTGGAA CTGAGTCAAA GGAAGCTGAG TCGTCTGAGG 
AACAGAAAGC TGTTGGATGA CTGGACGACA GTGGAGGAGC TGCTGACTCA TGGGACCAGG 
CTGGACAACC AGGAGGACAT GCTGTGTCCA AGCTCCCTGC TCACAGTTAC CACTGTCTAA