LOC106521892 cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106521892
Entrez Gene ID	106521892
Full Name	phospholipid-transporting ATPase ID-like
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014014652.1	XP_013870106.1	phospholipid-transporting ATPase ID-like isoform X2
XM_014014651.1	XP_013870105.1	phospholipid-transporting ATPase ID-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC106521892 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106521892 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu14043	XM_014014651.1 Latest version!	Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAu14044	XM_014014652.1 Latest version!	Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106521892 ( XM_014014651.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013870105.1 Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAu14043
Clone ID Related Accession (Same CDS sequence)	XM_014014651.1
Accession Version	XM_014014651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	phospholipid-transporting ATPase ID-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952519.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCT TCGGTCTGGA TTGTGTCACA AAGACAGAGA GAGAGCTGGA GAGGAAACTG 
AGAGCAAACG ACCGCGAGTA CAACCGCTCC TTCAAATACG CAAAAAATGC CATCAAGACC 
TCCAAGTACA ACGTCTTCAC CTTCCTTCCT CTCAACCTGT TTGAACAGTT TCAGAGGATC 
GCCAACGCCT ACTTTCTTGT TCTGCTGGTG CTTCAGGTGA TTCCTCAGAT CTCCTCCCTG 
TCCTGGTTCA CCACCGTCGT GCCGCTCATC TTCGTGCTGA CCGTCACGGC GGCCAAGGAT 
GCCACTGACG ACCTCAATCG CCACAGGAGC GACAAACGAG TAAACAATCG AAAGGTTCAA 
GTTCTTATTG ACAGAAAGCT GCGCAGCGAG AAATGGATGG ACGTCCAGGT CGGAGACATC 
ATCAAGCTGG AAAACAACCA GTTTGTCACC GCTGACCTCT TGCTGCTCTC CAGCAGCGAA 
CCACTCAACC TTGTGTATAT CGAAACAGCA GAACTGGACG GTGAGACGAA CCTGAAGGTG 
AGACAGGCCC TGACAGTGAC AGGAGACCTG GGAGATGACG TTGAAAAACT GGCAGACTTT 
GACGGTGAGG TGCGCTGTGA GGCCCCCAAC AACCGCCTCG ACCGTTTCGC GGGAACACTG 
ACCTTCGCCG GACAGAAATA TGCTTTGGAC AACGAGAAAA TCCTGCTGCG AGGCTGCACC 
CTGAGGAACA CCGAGTGGTG CTTTGGGCTG GTGCTGTTCG CAGGTCAGGA AACAAAACTG 
ATGCAGAACT GTGGGAAGAG CACATTCAAG AGAACCAGTG TAGACCGACT GATGAACGTT 
TTAGTCCTCT GTATCTTTGG TTTTCTCGTC TGCATGTGCA CGGTTCTGGC GATAGGAAAC 
TACATCTGGG AGATAAACAA AGGCTCCAAC TTCACCGTCT TCCTGCCGAG GGAGGATGGA 
AACACTGCTG GTTTCTCTTC TTTCCTCACC TTCTGGTCCT ACGTCATCAT CCTCAACACT 
GTGGTGCCCA TTTCTCTTTA TGTTAGCGTG GAGATTATTC GCCTGGGGAA CAGCTTCTAC 
ATCGACTGGG ACAGGAAGAT GTACCATTCT CACAGCGACA CGCCCGCCGA GGCTCGGACC 
ACCACGCTGA ACGAGGAGCT GGGCCAGATC AAATACATCT TTAGTGACAA AACAGGAACT 
TTGACCCAGA ACATCATGAT CTTCAACAAG TGCTCCATCA ACGGCAGATC TTACGGAGAC 
GTGTACGACT ACACAGGACA GAGACTTGAA ATCTCTGAGC AGAAAGACTC GGTGGACTTC 
TCTTTCAACC CACTGGCTGA CCCCCGGTTT GTGTTCTACG ACCACTCCCT GGTGGAGGCG 
GTGAAGCTGG AGAACCCAGA GGTCCACGCC TTCTTCAGGC TGCTGGCGCT CTGCCACACC 
GTCATGGCCG GGGAGAAGAA AGAAGGTGAT GATCAAGGTG AGCTTTACTA CCAGGCCCAG 
TCACCAGATG AGGGAGCTCT GGTGACAGCA GCCAGAAACT TCGGGTTCGT CTTCCGCTCG 
CGAACACCGG ACAGCATTTC CATCGTGGAG ATGGGGAAGC AGCGCAGCTA CGAACTCCTG 
GCAATCCTGG ATTTTAACAA CGTTCGCAAG AGGATGTCTG TCATCGTTCG GAATCCGGAG 
GGGAAACTGT CTCTGTACTG TAAAGGTGCC GACACCATCG TCTACGAGAG GCTTCACCAG 
TCCTGCAGCA AACTGATGGA CGTCACGACA GAGCACCTAA ACGAATTTGC AGGCGAGGGC 
CTCCGCACTC TCGCTCTGGC CTACAAGGAT TTGGACGAGG AGTATTTCAA CGGGTGGAAA 
CAGCGCCACC ATGAGGCGAG CACCGAGCTG GACGACAGGG AGAGCAAACT GGACGCGCTG 
TACGAGGAGA TAGAGAAGGA TCTGCTGCTG CTGGGAGCCA CGGCCATAGA AGACAAGTTA 
CAGGACGGAG TTCCTCAGAC CATCGAGCAG CTGTCCAAAG CTGACATCAA AGTCTGGGTT 
CTGACCGGTG ACAAGCAAGA AACAGCAGAA AACATCGGTT ACTCGTGCAA CTTACTGCGC 
GAAGAGATGA ACGAGGTTTT TATCGTCTCA GGAAGCTCTT CTGATGATGT CAGACAAGAA 
CTCAGAAATG CACAGACCTC CATGAAACCA AGCGCAGCAG AGAACTCTGT GTTTTTACCT 
GAAGAGAAGG GTGAGGGAGT GAAACCGATG AAAGATGAGG TGGTTAATGG AGAGTACGGC 
CTTGTTATCA ACGGACACAG TCTGGCGTTC GCTCTGGAGC GCAGCACGGA GCTGGACTTC 
CTCCGGACGG CCTGCATGTG TAAATCAGTG ATCTGCTGCA GGGTGACCCC TCTGCAGAAG 
GCTCAGGTGG TCGAACTGGT CAAGAAATAC AAGCAGGCGG TCACGTTGGC CATAGGTGAT 
GGAGCGAATG ATGTCAGCAT GATCAAAGCG GCTCATATAG GCGTGGGCAT CTCGGGTCAA 
GAGGGCATGC AGGCGGTCCT GTCCAGCGAC TACTCCTTCG CCCAGTTCCA CTTCCTGCAG 
CGCCTCCTGC TGGTGCACGG CCGCTGGTCC TACCTGCGCA TGTGCAAGTT CCTGCGCTAC 
TTCTTCTACA AGAACTTCAC CTTCACCTTC GTCCACTTCT GGTTCGCTTT CTTCTGTGGC 
TTCTCTGCAC AGACGGTGTA TGACGAGTGG TTTATCACCC TCTACAATCT GATGTACACT 
GCACTACCTG TTCTGGGAAT GAGCTTGTTT GATCAGGATG TGAACGACGT GTGGAGTTTC 
CAGCATCCGC AGCTGTACGT TCCGGGTCAG CTGAATCTTT ACTTCAGCAA GAAGGCCTTC 
TTTAAATGTG CCATCCACAG CTGCTACAGC TCCCTGGTGC TCTTCTTCAT CCCCTACGCT 
GCTTTACAGG ACACAGTGAG GGATGATGGG AAGGACGTGG CCAACTACCA GTCCTTCGCC 
CTGCTCACAC AGACCAGTCT GCTGTTTGCA GTCAGCATCC AGCTGGGCTT GGAGATGTCT 
TACTGGACGG CGGTGAACAC CTTCTTCGTT TTGGGCAGCT TGGTCATGTA TTTTGTCGTC 
ACGTTCACCA TGTACAGCGA CGGCATGTTC CTCCTCTTAC CGTCAGCGTT TCCTTTCATA 
GGATCGGCTC GGAACTCGCT GAACCAGCCC AACGTTTGGC TGACCATCGT CCTCACCTCC 
ATCCTGTGTG TCCTTCCTGT GGTGACGTAC CGCTTCCTGT TGATCCAGCT CTGCCCCACC 
ATTAATGACA AGGTGATGTT CAAAGTCAGG CAGGCCAAAG CGACTCTTCC CCCTCCGCCT 
CGCCGGACCC GGATCCGCCG CAGCAGCACC CGCCGCTCGG GCTACGCCTT CTCGCACGCG 
CAGGGCTATG GGGAGCTGGT GACCTCAGGT CGGTTCCTGC GGCGGCCCGC CATAAGATCC 
GCAGGCTTCC CCCAGGTGGG TCGGACCACC ACGGGCTTCA GCCCCATGGG CCGTTCGGCC 
GGTTACAGCC CGACGGGACG TCCCCAGAAC TTAAAGGTCT CGGATGTGGA GGTGACGTCG 
CTGCAGATGT ACAGGACGAT CACAGACCCG CCCCTCTCGC ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013870105.1
CDS	271..3918
Translation	MSFFGLDCVTKTERELERKLRANDREYNRSFKYAKNAIKTSKYNVFTFLPLNLFEQFQRIANAYFLVLLVLQVIPQISSLSWFTTVVPLIFVLTVTAAKDATDDLNRHRSDKRVNNRKVQVLIDRKLRSEKWMDVQVGDIIKLENNQFVTADLLLLSSSEPLNLVYIETAELDGETNLKVRQALTVTGDLGDDVEKLADFDGEVRCEAPNNRLDRFAGTLTFAGQKYALDNEKILLRGCTLRNTEWCFGLVLFAGQETKLMQNCGKSTFKRTSVDRLMNVLVLCIFGFLVCMCTVLAIGNYIWEINKGSNFTVFLPREDGNTAGFSSFLTFWSYVIILNTVVPISLYVSVEIIRLGNSFYIDWDRKMYHSHSDTPAEARTTTLNEELGQIKYIFSDKTGTLTQNIMIFNKCSINGRSYGDVYDYTGQRLEISEQKDSVDFSFNPLADPRFVFYDHSLVEAVKLENPEVHAFFRLLALCHTVMAGEKKEGDDQGELYYQAQSPDEGALVTAARNFGFVFRSRTPDSISIVEMGKQRSYELLAILDFNNVRKRMSVIVRNPEGKLSLYCKGADTIVYERLHQSCSKLMDVTTEHLNEFAGEGLRTLALAYKDLDEEYFNGWKQRHHEASTELDDRESKLDALYEEIEKDLLLLGATAIEDKLQDGVPQTIEQLSKADIKVWVLTGDKQETAENIGYSCNLLREEMNEVFIVSGSSSDDVRQELRNAQTSMKPSAAENSVFLPEEKGEGVKPMKDEVVNGEYGLVINGHSLAFALERSTELDFLRTACMCKSVICCRVTPLQKAQVVELVKKYKQAVTLAIGDGANDVSMIKAAHIGVGISGQEGMQAVLSSDYSFAQFHFLQRLLLVHGRWSYLRMCKFLRYFFYKNFTFTFVHFWFAFFCGFSAQTVYDEWFITLYNLMYTALPVLGMSLFDQDVNDVWSFQHPQLYVPGQLNLYFSKKAFFKCAIHSCYSSLVLFFIPYAALQDTVRDDGKDVANYQSFALLTQTSLLFAVSIQLGLEMSYWTAVNTFFVLGSLVMYFVVTFTMYSDGMFLLLPSAFPFIGSARNSLNQPNVWLTIVLTSILCVLPVVTYRFLLIQLCPTINDKVMFKVRQAKATLPPPPRRTRIRRSSTRRSGYAFSHAQGYGELVTSGRFLRRPAIRSAGFPQVGRTTTGFSPMGRSAGYSPTGRPQNLKVSDVEVTSLQMYRTITDPPLSHT

Target ORF information:

RefSeq Version	XM_014014651.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014014651.1

ORF Insert Sequence:

ATGTCGTTCT TCGGTCTGGA TTGTGTCACA AAGACAGAGA GAGAGCTGGA GAGGAAACTG 
AGAGCAAACG ACCGCGAGTA CAACCGCTCC TTCAAATACG CAAAAAATGC CATCAAGACC 
TCCAAGTACA ACGTCTTCAC CTTCCTTCCT CTCAACCTGT TTGAACAGTT TCAGAGGATC 
GCCAACGCCT ACTTTCTTGT TCTGCTGGTG CTTCAGGTGA TTCCTCAGAT CTCCTCCCTG 
TCCTGGTTCA CCACCGTCGT GCCGCTCATC TTCGTGCTGA CCGTCACGGC GGCCAAGGAT 
GCCACTGACG ACCTCAATCG CCACAGGAGC GACAAACGAG TAAACAATCG AAAGGTTCAA 
GTTCTTATTG ACAGAAAGCT GCGCAGCGAG AAATGGATGG ACGTCCAGGT CGGAGACATC 
ATCAAGCTGG AAAACAACCA GTTTGTCACC GCTGACCTCT TGCTGCTCTC CAGCAGCGAA 
CCACTCAACC TTGTGTATAT CGAAACAGCA GAACTGGACG GTGAGACGAA CCTGAAGGTG 
AGACAGGCCC TGACAGTGAC AGGAGACCTG GGAGATGACG TTGAAAAACT GGCAGACTTT 
GACGGTGAGG TGCGCTGTGA GGCCCCCAAC AACCGCCTCG ACCGTTTCGC GGGAACACTG 
ACCTTCGCCG GACAGAAATA TGCTTTGGAC AACGAGAAAA TCCTGCTGCG AGGCTGCACC 
CTGAGGAACA CCGAGTGGTG CTTTGGGCTG GTGCTGTTCG CAGGTCAGGA AACAAAACTG 
ATGCAGAACT GTGGGAAGAG CACATTCAAG AGAACCAGTG TAGACCGACT GATGAACGTT 
TTAGTCCTCT GTATCTTTGG TTTTCTCGTC TGCATGTGCA CGGTTCTGGC GATAGGAAAC 
TACATCTGGG AGATAAACAA AGGCTCCAAC TTCACCGTCT TCCTGCCGAG GGAGGATGGA 
AACACTGCTG GTTTCTCTTC TTTCCTCACC TTCTGGTCCT ACGTCATCAT CCTCAACACT 
GTGGTGCCCA TTTCTCTTTA TGTTAGCGTG GAGATTATTC GCCTGGGGAA CAGCTTCTAC 
ATCGACTGGG ACAGGAAGAT GTACCATTCT CACAGCGACA CGCCCGCCGA GGCTCGGACC 
ACCACGCTGA ACGAGGAGCT GGGCCAGATC AAATACATCT TTAGTGACAA AACAGGAACT 
TTGACCCAGA ACATCATGAT CTTCAACAAG TGCTCCATCA ACGGCAGATC TTACGGAGAC 
GTGTACGACT ACACAGGACA GAGACTTGAA ATCTCTGAGC AGAAAGACTC GGTGGACTTC 
TCTTTCAACC CACTGGCTGA CCCCCGGTTT GTGTTCTACG ACCACTCCCT GGTGGAGGCG 
GTGAAGCTGG AGAACCCAGA GGTCCACGCC TTCTTCAGGC TGCTGGCGCT CTGCCACACC 
GTCATGGCCG GGGAGAAGAA AGAAGGTGAT GATCAAGGTG AGCTTTACTA CCAGGCCCAG 
TCACCAGATG AGGGAGCTCT GGTGACAGCA GCCAGAAACT TCGGGTTCGT CTTCCGCTCG 
CGAACACCGG ACAGCATTTC CATCGTGGAG ATGGGGAAGC AGCGCAGCTA CGAACTCCTG 
GCAATCCTGG ATTTTAACAA CGTTCGCAAG AGGATGTCTG TCATCGTTCG GAATCCGGAG 
GGGAAACTGT CTCTGTACTG TAAAGGTGCC GACACCATCG TCTACGAGAG GCTTCACCAG 
TCCTGCAGCA AACTGATGGA CGTCACGACA GAGCACCTAA ACGAATTTGC AGGCGAGGGC 
CTCCGCACTC TCGCTCTGGC CTACAAGGAT TTGGACGAGG AGTATTTCAA CGGGTGGAAA 
CAGCGCCACC ATGAGGCGAG CACCGAGCTG GACGACAGGG AGAGCAAACT GGACGCGCTG 
TACGAGGAGA TAGAGAAGGA TCTGCTGCTG CTGGGAGCCA CGGCCATAGA AGACAAGTTA 
CAGGACGGAG TTCCTCAGAC CATCGAGCAG CTGTCCAAAG CTGACATCAA AGTCTGGGTT 
CTGACCGGTG ACAAGCAAGA AACAGCAGAA AACATCGGTT ACTCGTGCAA CTTACTGCGC 
GAAGAGATGA ACGAGGTTTT TATCGTCTCA GGAAGCTCTT CTGATGATGT CAGACAAGAA 
CTCAGAAATG CACAGACCTC CATGAAACCA AGCGCAGCAG AGAACTCTGT GTTTTTACCT 
GAAGAGAAGG GTGAGGGAGT GAAACCGATG AAAGATGAGG TGGTTAATGG AGAGTACGGC 
CTTGTTATCA ACGGACACAG TCTGGCGTTC GCTCTGGAGC GCAGCACGGA GCTGGACTTC 
CTCCGGACGG CCTGCATGTG TAAATCAGTG ATCTGCTGCA GGGTGACCCC TCTGCAGAAG 
GCTCAGGTGG TCGAACTGGT CAAGAAATAC AAGCAGGCGG TCACGTTGGC CATAGGTGAT 
GGAGCGAATG ATGTCAGCAT GATCAAAGCG GCTCATATAG GCGTGGGCAT CTCGGGTCAA 
GAGGGCATGC AGGCGGTCCT GTCCAGCGAC TACTCCTTCG CCCAGTTCCA CTTCCTGCAG 
CGCCTCCTGC TGGTGCACGG CCGCTGGTCC TACCTGCGCA TGTGCAAGTT CCTGCGCTAC 
TTCTTCTACA AGAACTTCAC CTTCACCTTC GTCCACTTCT GGTTCGCTTT CTTCTGTGGC 
TTCTCTGCAC AGACGGTGTA TGACGAGTGG TTTATCACCC TCTACAATCT GATGTACACT 
GCACTACCTG TTCTGGGAAT GAGCTTGTTT GATCAGGATG TGAACGACGT GTGGAGTTTC 
CAGCATCCGC AGCTGTACGT TCCGGGTCAG CTGAATCTTT ACTTCAGCAA GAAGGCCTTC 
TTTAAATGTG CCATCCACAG CTGCTACAGC TCCCTGGTGC TCTTCTTCAT CCCCTACGCT 
GCTTTACAGG ACACAGTGAG GGATGATGGG AAGGACGTGG CCAACTACCA GTCCTTCGCC 
CTGCTCACAC AGACCAGTCT GCTGTTTGCA GTCAGCATCC AGCTGGGCTT GGAGATGTCT 
TACTGGACGG CGGTGAACAC CTTCTTCGTT TTGGGCAGCT TGGTCATGTA TTTTGTCGTC 
ACGTTCACCA TGTACAGCGA CGGCATGTTC CTCCTCTTAC CGTCAGCGTT TCCTTTCATA 
GGATCGGCTC GGAACTCGCT GAACCAGCCC AACGTTTGGC TGACCATCGT CCTCACCTCC 
ATCCTGTGTG TCCTTCCTGT GGTGACGTAC CGCTTCCTGT TGATCCAGCT CTGCCCCACC 
ATTAATGACA AGGTGATGTT CAAAGTCAGG CAGGCCAAAG CGACTCTTCC CCCTCCGCCT 
CGCCGGACCC GGATCCGCCG CAGCAGCACC CGCCGCTCGG GCTACGCCTT CTCGCACGCG 
CAGGGCTATG GGGAGCTGGT GACCTCAGGT CGGTTCCTGC GGCGGCCCGC CATAAGATCC 
GCAGGCTTCC CCCAGGTGGG TCGGACCACC ACGGGCTTCA GCCCCATGGG CCGTTCGGCC 
GGTTACAGCC CGACGGGACG TCCCCAGAAC TTAAAGGTCT CGGATGTGGA GGTGACGTCG 
CTGCAGATGT ACAGGACGAT CACAGACCCG CCCCTCTCGC ACACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106521892 ( XM_014014652.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013870106.1 Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAu14044
Clone ID Related Accession (Same CDS sequence)	XM_014014652.1
Accession Version	XM_014014652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	phospholipid-transporting ATPase ID-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952519.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGTTCT TCGGTCTGGA TTGTGTCACA AAGACAGAGA GAGAGCTGGA GAGGAAACTG 
AGAGCAAACG ACCGCGAGTA CAACCGCTCC TTCAAATACG CAAAAAATGC CATCAAGACC 
TCCAAGTACA ACGTCTTCAC CTTCCTTCCT CTCAACCTGT TTGAACAGTT TCAGAGGATC 
GCCAACGCCT ACTTTCTTGT TCTGCTGGTG CTTCAGGTGA TTCCTCAGAT CTCCTCCCTG 
TCCTGGTTCA CCACCGTCGT GCCGCTCATC TTCGTGCTGA CCGTCACGGC GGCCAAGGAT 
GCCACTGACG ACCTCAATCG CCACAGGAGC GACAAACGAG TAAACAATCG AAAGGTTCAA 
GTTCTTATTG ACAGAAAGCT GCGCAGCGAG AAATGGATGG ACGTCCAGGT CGGAGACATC 
ATCAAGCTGG AAAACAACCA GTTTGTCACC GCTGACCTCT TGCTGCTCTC CAGCAGCGAA 
CCACTCAACC TTGTGTATAT CGAAACAGCA GAACTGGACG GTGAGACGAA CCTGAAGGTG 
AGACAGGCCC TGACAGTGAC AGGAGACCTG GGAGATGACG TTGAAAAACT GGCAGACTTT 
GACGGTGAGG TGCGCTGTGA GGCCCCCAAC AACCGCCTCG ACCGTTTCGC GGGAACACTG 
ACCTTCGCCG GACAGAAATA TGCTTTGGAC AACGAGAAAA TCCTGCTGCG AGGCTGCACC 
CTGAGGAACA CCGAGTGGTG CTTTGGGCTG GTGCTGTTCG CAGGTCAGGA AACAAAACTG 
ATGCAGAACT GTGGGAAGAG CACATTCAAG AGAACCAGTG TAGACCGACT GATGAACGTT 
TTAGTCCTCT GTATCTTTGG TTTTCTCGTC TGCATGTGCA CGGTTCTGGC GATAGGAAAC 
TACATCTGGG AGATAAACAA AGGCTCCAAC TTCACCGTCT TCCTGCCGAG GGAGGATGGA 
AACACTGCTG GTTTCTCTTC TTTCCTCACC TTCTGGTCCT ACGTCATCAT CCTCAACACT 
GTGGTGCCCA TTTCTCTTTA TGTTAGCGTG GAGATTATTC GCCTGGGGAA CAGCTTCTAC 
ATCGACTGGG ACAGGAAGAT GTACCATTCT CACAGCGACA CGCCCGCCGA GGCTCGGACC 
ACCACGCTGA ACGAGGAGCT GGGCCAGATC AAATACATCT TTAGTGACAA AACAGGAACT 
TTGACCCAGA ACATCATGAT CTTCAACAAG TGCTCCATCA ACGGCAGATC TTACGGAGAC 
GTGTACGACT ACACAGGACA GAGACTTGAA ATCTCTGAGC AGAAAGACTC GGTGGACTTC 
TCTTTCAACC CACTGGCTGA CCCCCGGTTT GTGTTCTACG ACCACTCCCT GGTGGAGGCG 
GTGAAGCTGG AGAACCCAGA GGTCCACGCC TTCTTCAGGC TGCTGGCGCT CTGCCACACC 
GTCATGGCCG GGGAGAAGAA AGAAGGTGAG CTTTACTACC AGGCCCAGTC ACCAGATGAG 
GGAGCTCTGG TGACAGCAGC CAGAAACTTC GGGTTCGTCT TCCGCTCGCG AACACCGGAC 
AGCATTTCCA TCGTGGAGAT GGGGAAGCAG CGCAGCTACG AACTCCTGGC AATCCTGGAT 
TTTAACAACG TTCGCAAGAG GATGTCTGTC ATCGTTCGGA ATCCGGAGGG GAAACTGTCT 
CTGTACTGTA AAGGTGCCGA CACCATCGTC TACGAGAGGC TTCACCAGTC CTGCAGCAAA 
CTGATGGACG TCACGACAGA GCACCTAAAC GAATTTGCAG GCGAGGGCCT CCGCACTCTC 
GCTCTGGCCT ACAAGGATTT GGACGAGGAG TATTTCAACG GGTGGAAACA GCGCCACCAT 
GAGGCGAGCA CCGAGCTGGA CGACAGGGAG AGCAAACTGG ACGCGCTGTA CGAGGAGATA 
GAGAAGGATC TGCTGCTGCT GGGAGCCACG GCCATAGAAG ACAAGTTACA GGACGGAGTT 
CCTCAGACCA TCGAGCAGCT GTCCAAAGCT GACATCAAAG TCTGGGTTCT GACCGGTGAC 
AAGCAAGAAA CAGCAGAAAA CATCGGTTAC TCGTGCAACT TACTGCGCGA AGAGATGAAC 
GAGGTTTTTA TCGTCTCAGG AAGCTCTTCT GATGATGTCA GACAAGAACT CAGAAATGCA 
CAGACCTCCA TGAAACCAAG CGCAGCAGAG AACTCTGTGT TTTTACCTGA AGAGAAGGGT 
GAGGGAGTGA AACCGATGAA AGATGAGGTG GTTAATGGAG AGTACGGCCT TGTTATCAAC 
GGACACAGTC TGGCGTTCGC TCTGGAGCGC AGCACGGAGC TGGACTTCCT CCGGACGGCC 
TGCATGTGTA AATCAGTGAT CTGCTGCAGG GTGACCCCTC TGCAGAAGGC TCAGGTGGTC 
GAACTGGTCA AGAAATACAA GCAGGCGGTC ACGTTGGCCA TAGGTGATGG AGCGAATGAT 
GTCAGCATGA TCAAAGCGGC TCATATAGGC GTGGGCATCT CGGGTCAAGA GGGCATGCAG 
GCGGTCCTGT CCAGCGACTA CTCCTTCGCC CAGTTCCACT TCCTGCAGCG CCTCCTGCTG 
GTGCACGGCC GCTGGTCCTA CCTGCGCATG TGCAAGTTCC TGCGCTACTT CTTCTACAAG 
AACTTCACCT TCACCTTCGT CCACTTCTGG TTCGCTTTCT TCTGTGGCTT CTCTGCACAG 
ACGGTGTATG ACGAGTGGTT TATCACCCTC TACAATCTGA TGTACACTGC ACTACCTGTT 
CTGGGAATGA GCTTGTTTGA TCAGGATGTG AACGACGTGT GGAGTTTCCA GCATCCGCAG 
CTGTACGTTC CGGGTCAGCT GAATCTTTAC TTCAGCAAGA AGGCCTTCTT TAAATGTGCC 
ATCCACAGCT GCTACAGCTC CCTGGTGCTC TTCTTCATCC CCTACGCTGC TTTACAGGAC 
ACAGTGAGGG ATGATGGGAA GGACGTGGCC AACTACCAGT CCTTCGCCCT GCTCACACAG 
ACCAGTCTGC TGTTTGCAGT CAGCATCCAG CTGGGCTTGG AGATGTCTTA CTGGACGGCG 
GTGAACACCT TCTTCGTTTT GGGCAGCTTG GTCATGTATT TTGTCGTCAC GTTCACCATG 
TACAGCGACG GCATGTTCCT CCTCTTACCG TCAGCGTTTC CTTTCATAGG ATCGGCTCGG 
AACTCGCTGA ACCAGCCCAA CGTTTGGCTG ACCATCGTCC TCACCTCCAT CCTGTGTGTC 
CTTCCTGTGG TGACGTACCG CTTCCTGTTG ATCCAGCTCT GCCCCACCAT TAATGACAAG 
GTGATGTTCA AAGTCAGGCA GGCCAAAGCG ACTCTTCCCC CTCCGCCTCG CCGGACCCGG 
ATCCGCCGCA GCAGCACCCG CCGCTCGGGC TACGCCTTCT CGCACGCGCA GGGCTATGGG 
GAGCTGGTGA CCTCAGGTCG GTTCCTGCGG CGGCCCGCCA TAAGATCCGC AGGCTTCCCC 
CAGGTGGGTC GGACCACCAC GGGCTTCAGC CCCATGGGCC GTTCGGCCGG TTACAGCCCG 
ACGGGACGTC CCCAGAACTT AAAGGTCTCG GATGTGGAGG TGACGTCGCT GCAGATGTAC 
AGGACGATCA CAGACCCGCC CCTCTCGCAC ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013870106.1
CDS	270..3905
Translation	MSFFGLDCVTKTERELERKLRANDREYNRSFKYAKNAIKTSKYNVFTFLPLNLFEQFQRIANAYFLVLLVLQVIPQISSLSWFTTVVPLIFVLTVTAAKDATDDLNRHRSDKRVNNRKVQVLIDRKLRSEKWMDVQVGDIIKLENNQFVTADLLLLSSSEPLNLVYIETAELDGETNLKVRQALTVTGDLGDDVEKLADFDGEVRCEAPNNRLDRFAGTLTFAGQKYALDNEKILLRGCTLRNTEWCFGLVLFAGQETKLMQNCGKSTFKRTSVDRLMNVLVLCIFGFLVCMCTVLAIGNYIWEINKGSNFTVFLPREDGNTAGFSSFLTFWSYVIILNTVVPISLYVSVEIIRLGNSFYIDWDRKMYHSHSDTPAEARTTTLNEELGQIKYIFSDKTGTLTQNIMIFNKCSINGRSYGDVYDYTGQRLEISEQKDSVDFSFNPLADPRFVFYDHSLVEAVKLENPEVHAFFRLLALCHTVMAGEKKEGELYYQAQSPDEGALVTAARNFGFVFRSRTPDSISIVEMGKQRSYELLAILDFNNVRKRMSVIVRNPEGKLSLYCKGADTIVYERLHQSCSKLMDVTTEHLNEFAGEGLRTLALAYKDLDEEYFNGWKQRHHEASTELDDRESKLDALYEEIEKDLLLLGATAIEDKLQDGVPQTIEQLSKADIKVWVLTGDKQETAENIGYSCNLLREEMNEVFIVSGSSSDDVRQELRNAQTSMKPSAAENSVFLPEEKGEGVKPMKDEVVNGEYGLVINGHSLAFALERSTELDFLRTACMCKSVICCRVTPLQKAQVVELVKKYKQAVTLAIGDGANDVSMIKAAHIGVGISGQEGMQAVLSSDYSFAQFHFLQRLLLVHGRWSYLRMCKFLRYFFYKNFTFTFVHFWFAFFCGFSAQTVYDEWFITLYNLMYTALPVLGMSLFDQDVNDVWSFQHPQLYVPGQLNLYFSKKAFFKCAIHSCYSSLVLFFIPYAALQDTVRDDGKDVANYQSFALLTQTSLLFAVSIQLGLEMSYWTAVNTFFVLGSLVMYFVVTFTMYSDGMFLLLPSAFPFIGSARNSLNQPNVWLTIVLTSILCVLPVVTYRFLLIQLCPTINDKVMFKVRQAKATLPPPPRRTRIRRSSTRRSGYAFSHAQGYGELVTSGRFLRRPAIRSAGFPQVGRTTTGFSPMGRSAGYSPTGRPQNLKVSDVEVTSLQMYRTITDPPLSHT

Target ORF information:

RefSeq Version	XM_014014652.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus phospholipid-transporting ATPase ID-like (LOC106521892), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014014652.1

ORF Insert Sequence:

ATGTCGTTCT TCGGTCTGGA TTGTGTCACA AAGACAGAGA GAGAGCTGGA GAGGAAACTG 
AGAGCAAACG ACCGCGAGTA CAACCGCTCC TTCAAATACG CAAAAAATGC CATCAAGACC 
TCCAAGTACA ACGTCTTCAC CTTCCTTCCT CTCAACCTGT TTGAACAGTT TCAGAGGATC 
GCCAACGCCT ACTTTCTTGT TCTGCTGGTG CTTCAGGTGA TTCCTCAGAT CTCCTCCCTG 
TCCTGGTTCA CCACCGTCGT GCCGCTCATC TTCGTGCTGA CCGTCACGGC GGCCAAGGAT 
GCCACTGACG ACCTCAATCG CCACAGGAGC GACAAACGAG TAAACAATCG AAAGGTTCAA 
GTTCTTATTG ACAGAAAGCT GCGCAGCGAG AAATGGATGG ACGTCCAGGT CGGAGACATC 
ATCAAGCTGG AAAACAACCA GTTTGTCACC GCTGACCTCT TGCTGCTCTC CAGCAGCGAA 
CCACTCAACC TTGTGTATAT CGAAACAGCA GAACTGGACG GTGAGACGAA CCTGAAGGTG 
AGACAGGCCC TGACAGTGAC AGGAGACCTG GGAGATGACG TTGAAAAACT GGCAGACTTT 
GACGGTGAGG TGCGCTGTGA GGCCCCCAAC AACCGCCTCG ACCGTTTCGC GGGAACACTG 
ACCTTCGCCG GACAGAAATA TGCTTTGGAC AACGAGAAAA TCCTGCTGCG AGGCTGCACC 
CTGAGGAACA CCGAGTGGTG CTTTGGGCTG GTGCTGTTCG CAGGTCAGGA AACAAAACTG 
ATGCAGAACT GTGGGAAGAG CACATTCAAG AGAACCAGTG TAGACCGACT GATGAACGTT 
TTAGTCCTCT GTATCTTTGG TTTTCTCGTC TGCATGTGCA CGGTTCTGGC GATAGGAAAC 
TACATCTGGG AGATAAACAA AGGCTCCAAC TTCACCGTCT TCCTGCCGAG GGAGGATGGA 
AACACTGCTG GTTTCTCTTC TTTCCTCACC TTCTGGTCCT ACGTCATCAT CCTCAACACT 
GTGGTGCCCA TTTCTCTTTA TGTTAGCGTG GAGATTATTC GCCTGGGGAA CAGCTTCTAC 
ATCGACTGGG ACAGGAAGAT GTACCATTCT CACAGCGACA CGCCCGCCGA GGCTCGGACC 
ACCACGCTGA ACGAGGAGCT GGGCCAGATC AAATACATCT TTAGTGACAA AACAGGAACT 
TTGACCCAGA ACATCATGAT CTTCAACAAG TGCTCCATCA ACGGCAGATC TTACGGAGAC 
GTGTACGACT ACACAGGACA GAGACTTGAA ATCTCTGAGC AGAAAGACTC GGTGGACTTC 
TCTTTCAACC CACTGGCTGA CCCCCGGTTT GTGTTCTACG ACCACTCCCT GGTGGAGGCG 
GTGAAGCTGG AGAACCCAGA GGTCCACGCC TTCTTCAGGC TGCTGGCGCT CTGCCACACC 
GTCATGGCCG GGGAGAAGAA AGAAGGTGAG CTTTACTACC AGGCCCAGTC ACCAGATGAG 
GGAGCTCTGG TGACAGCAGC CAGAAACTTC GGGTTCGTCT TCCGCTCGCG AACACCGGAC 
AGCATTTCCA TCGTGGAGAT GGGGAAGCAG CGCAGCTACG AACTCCTGGC AATCCTGGAT 
TTTAACAACG TTCGCAAGAG GATGTCTGTC ATCGTTCGGA ATCCGGAGGG GAAACTGTCT 
CTGTACTGTA AAGGTGCCGA CACCATCGTC TACGAGAGGC TTCACCAGTC CTGCAGCAAA 
CTGATGGACG TCACGACAGA GCACCTAAAC GAATTTGCAG GCGAGGGCCT CCGCACTCTC 
GCTCTGGCCT ACAAGGATTT GGACGAGGAG TATTTCAACG GGTGGAAACA GCGCCACCAT 
GAGGCGAGCA CCGAGCTGGA CGACAGGGAG AGCAAACTGG ACGCGCTGTA CGAGGAGATA 
GAGAAGGATC TGCTGCTGCT GGGAGCCACG GCCATAGAAG ACAAGTTACA GGACGGAGTT 
CCTCAGACCA TCGAGCAGCT GTCCAAAGCT GACATCAAAG TCTGGGTTCT GACCGGTGAC 
AAGCAAGAAA CAGCAGAAAA CATCGGTTAC TCGTGCAACT TACTGCGCGA AGAGATGAAC 
GAGGTTTTTA TCGTCTCAGG AAGCTCTTCT GATGATGTCA GACAAGAACT CAGAAATGCA 
CAGACCTCCA TGAAACCAAG CGCAGCAGAG AACTCTGTGT TTTTACCTGA AGAGAAGGGT 
GAGGGAGTGA AACCGATGAA AGATGAGGTG GTTAATGGAG AGTACGGCCT TGTTATCAAC 
GGACACAGTC TGGCGTTCGC TCTGGAGCGC AGCACGGAGC TGGACTTCCT CCGGACGGCC 
TGCATGTGTA AATCAGTGAT CTGCTGCAGG GTGACCCCTC TGCAGAAGGC TCAGGTGGTC 
GAACTGGTCA AGAAATACAA GCAGGCGGTC ACGTTGGCCA TAGGTGATGG AGCGAATGAT 
GTCAGCATGA TCAAAGCGGC TCATATAGGC GTGGGCATCT CGGGTCAAGA GGGCATGCAG 
GCGGTCCTGT CCAGCGACTA CTCCTTCGCC CAGTTCCACT TCCTGCAGCG CCTCCTGCTG 
GTGCACGGCC GCTGGTCCTA CCTGCGCATG TGCAAGTTCC TGCGCTACTT CTTCTACAAG 
AACTTCACCT TCACCTTCGT CCACTTCTGG TTCGCTTTCT TCTGTGGCTT CTCTGCACAG 
ACGGTGTATG ACGAGTGGTT TATCACCCTC TACAATCTGA TGTACACTGC ACTACCTGTT 
CTGGGAATGA GCTTGTTTGA TCAGGATGTG AACGACGTGT GGAGTTTCCA GCATCCGCAG 
CTGTACGTTC CGGGTCAGCT GAATCTTTAC TTCAGCAAGA AGGCCTTCTT TAAATGTGCC 
ATCCACAGCT GCTACAGCTC CCTGGTGCTC TTCTTCATCC CCTACGCTGC TTTACAGGAC 
ACAGTGAGGG ATGATGGGAA GGACGTGGCC AACTACCAGT CCTTCGCCCT GCTCACACAG 
ACCAGTCTGC TGTTTGCAGT CAGCATCCAG CTGGGCTTGG AGATGTCTTA CTGGACGGCG 
GTGAACACCT TCTTCGTTTT GGGCAGCTTG GTCATGTATT TTGTCGTCAC GTTCACCATG 
TACAGCGACG GCATGTTCCT CCTCTTACCG TCAGCGTTTC CTTTCATAGG ATCGGCTCGG 
AACTCGCTGA ACCAGCCCAA CGTTTGGCTG ACCATCGTCC TCACCTCCAT CCTGTGTGTC 
CTTCCTGTGG TGACGTACCG CTTCCTGTTG ATCCAGCTCT GCCCCACCAT TAATGACAAG 
GTGATGTTCA AAGTCAGGCA GGCCAAAGCG ACTCTTCCCC CTCCGCCTCG CCGGACCCGG 
ATCCGCCGCA GCAGCACCCG CCGCTCGGGC TACGCCTTCT CGCACGCGCA GGGCTATGGG 
GAGCTGGTGA CCTCAGGTCG GTTCCTGCGG CGGCCCGCCA TAAGATCCGC AGGCTTCCCC 
CAGGTGGGTC GGACCACCAC GGGCTTCAGC CCCATGGGCC GTTCGGCCGG TTACAGCCCG 
ACGGGACGTC CCCAGAACTT AAAGGTCTCG GATGTGGAGG TGACGTCGCT GCAGATGTAC 
AGGACGATCA CAGACCCGCC CCTCTCGCAC ACATGA