crim1 cDNA ORF clone, Astyanax mexicanus(Mexican tetra)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	103047393
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Astyanax mexicanus(Mexican tetra)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007252565.2	XP_007252627.1	cysteine-rich motor neuron 1 protein
XM_022662672.1	XP_022518393.1	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAs34753	XM_007252565.2 Latest version!	Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAs97534	XM_022662672.1 Latest version!	Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_007252565.2 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_007252627.1 Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

Price & Availability↑

CloneID	OAs34753
Clone ID Related Accession (Same CDS sequence)	XM_007252565.2
Accession Version	XM_007252565.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-10
Organism	Astyanax mexicanus(Mexican tetra)
Product	cysteine-rich motor neuron 1 protein
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006749345.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 11, 2016 this sequence version replaced XM_007252565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCACCCCT TGGTGAACCG CGTGCTGTTC CCGCAGCTGG CCGTGCTGCT GGTGCTGCGC 
GGCGGCGCGG CGCTGCTCTG CCTGCCCTGC GACGAGTCCA AGTGCGAGGA GCCCAAGCAG 
TGCACGGGCG CCGTGGTGCT GGGCATCTGC GGCTGCTGCT CCGTGTGCGC CAAGCAGAAG 
AACGAGAGCT GCGGCGGCGT GTACGGGCTG TACGGGACGT GCGACGCGGG GCTGCGCTGC 
GTCATCCGCC CTCCGCTCGA CGGAGCCTCG ATCACCCAGT ACGAGGTCGG AGTTTGCGAA 
GATGAAAACT GGGATGACGA CCAGCTCTTG GGATTTGAGC CGTGCAATGA GAACCTGGTT 
ACGGGCTGCA ACATCATCGA TGGGAAGTGT GAATGTGACA GCGTACGGAC GTGCAACAAC 
CCATTTGAGT TTGCCAGCCA GGAAGCCTGT CAGACAGCCC TGAGGAAGAT CGAAGAGGAG 
AGACCGGACT GTTCTAAGGC CAGGTGTGAA GTTCAGTTTT CTCCTCGGTG TCCGGAGGAC 
TCTGTGCTGA TCGAGGGTTA CGCCCCACCG GGGGAGTGCT GCCCCCTGCC CAGCCGCTGT 
GTGTGCAGCC CTGCGGGTTG TTTGAGAAAA GTGTGCCAGC CAGGCTACCT CAACATCCTG 
GTGTCCAAAA GCTCGGGCAA GCCTGGAGAG TGCTGTGACC TGTATGAGTG CAAACCAGCA 
CCCGTCCTGC CGGTCCAGTG TCCAGCTGAC AGCTACGAGA CCCAGGTTAG ACTGACAGCA 
GATGGTTGCT GCACCCTTCC TACAAGGTGC GAGTGTTTGC CCAGCGTCTG CACCTTCCCG 
CAGTGTTCGG CTGGAACGTC CCCTCACTTG GTCTCCCGTG GCAACGGAAC ACCAGGGCGG 
TGCTGTGATG TGTTTGAGTG TGTTAATGAG ACCAAGCCAG CCTGTACCTT CAACGGTGTG 
GAGTACCATG ATGGAGACAT GTTCAGGATG GACACCTGCC GCTTCTGCCG CTGCCAGGGC 
GGAGTGTCTG TGTGCTTCAC AGCGCAGTGC GGGGTCCTCC ACTGCGACCG CTACTACGTA 
CCTGAGGGGG AGTGCTGCCC TGTCTGTGAA GATCCCATCT TTCCAGCTCT GAACTTTGCA 
GGCTGCTATG TGAACGGTCA GATCCTGGCT CACGGTGACC ACTGGCGTGA GGATGACTGC 
ACGTTCTGCC AGTGTGTGAG CGGGGAGTCT CGTTGCGTGG CGGCTGCCTG CGGCCATAGC 
TGCCTCAACC CCGTCACGGT GCCCGGAGAA TGCTGCCCTG TGTGTGAGGA GCCCACCTAC 
ATCACCATGG CGCCCCCTGC CTGTGGCCCG CTGGAGAACT GCACGCTGAC GGTCCAGGAC 
TGCCTGTATG GTTTCCGGCT CGACCAGGAC GGCTGCCGCA CCTGCAGCTG CAAAACAAGA 
GAGGAGCTGT GCAGAGGCCT GATCTCCGGC TGCACGCTGA AGTGCCCGTT TGGTTTTCAG 
ACGGATGCTC ATGGCTGCAC CGTGTGCCAG TGCCGACAGC GACACAAGAA GTGCAAGCCG 
GTGGCCTGTG CCAAGGACTG TCCATTTGGC TTTGTCAAAA ACAAGCACGG CTGTGACACC 
TGTCGCTGTA AGAAGTGCCC CGAGCTGCCG TGTGACAAGC CGTGTCCAAT GGGCTTCCAG 
CAGGACGAGC TGGGCTGCCT GATATGCCAG TGTCGAGACC AGAGCTCCTC TTCTGTGGGC 
CCTTCGGTTA AGCTGGGATC GTGCCTCTCT ATGGATGGAC GCAGACATGA GAATGGTCAG 
AGTTGGCACG ACGGCTGCAG GGAGTGTTTT TGTCATGCCG GTCGAGAGAT GTGTGCACTT 
ATATCCTGCC CAGTGCCTTC CTGTGACAAC CCCAGCATCC GCGCCGGCCA GTGCTGCCCC 
ACCTGTCCAG ACGAGTCCAG CTCTCACAAG CCGGAGCTGA GTGAAGCGTC TGTGTGCTTG 
GCACCAGGTG GGGAGTATTT TGTGGAGGGC GAGACGTGGA ACATCGACTC CTGCACGCAG 
TGCACCTGCC ACGTCGGCCG CGTGCTCTGC GAGACCGAGG TGTGTCCGCC TCTGCTCTGC 
CAGAGCCCTA TCAGAACACA GGACTCCTGC TGCCCACACT GCCCAGATGA GCCGGTCACC 
CCTCCGGCCA CCAGCAACGT CAGCATGCCC AGCTACTGCC GCAACGAGGA GGGCGACATC 
TTCCTGGTGG CCGAGTCCTG GAAGCCCAAC GTCTGCTCCA GCTGTGTGTG TTTGGACGGA 
GTAATCAGCT GCTTCTCTGA ATCCTGCCCT CCGGTCAACT GCGCCCGGCC TGTCCTCCGG 
AAGGGCCAGT GCTGCCCCTA CTGCTTAGAT GCCACGCCAA GGGCCGTGTG CCACTTCAAC 
GGCAAGACCT ACGTGGACGA GGAGCGCTGG GACATCGACA GCTGCACGCA CTGCTACTGC 
CTGCAGGGTC AGACGCTCTG CTCCACCGTC AGCTGCCCTG CCCTGCCCTG CAGCCAACCC 
ATCACAGTGG AGGGCAGCTG CTGCTCCATG TGCCCAGAGA TCGCCCCCAC TAATGTTCCC 
ATAGAGAAGA CAGACCAACA TGGAGACAGA CTGAGGCATC GGCCGGCCTG GCCTACACAC 
AGCGAGAATG ACATAATGCC CCAGTTCAGA GGTGATTTCG GCAGCCTGCA GATGCCATAT 
CTGGATGGGA AGACGCCATT GCCCAGTGAG GATCCCAGTC TTCACTGGGC CTGGGTGGCT 
CTGCCCATCC TCATGATGGT GCTCACTATC GCCACCCTGC TGCTGATCAA TCAGAGAAAG 
CAGTGGATCC CAGTGCCTTG CTATCACACT CCCACTAAGC CCACGTGTCT GAATAACCAG 
CTGGTGTATG TGGACTGCCA GAAGGGCACA AAAGTTCAGG TGAACAGCTC TCAGCGCATG 
CTCCGCATCG CAGACCCCGA CTCGCGCTAC AGCGGCTACT ACAGCATGCC CAAACACAAC 
AACCTGCAAG CGGACAACTT CTACCAGACT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007252627.1
CDS	762..3797
Translation	MHPLVNRVLFPQLAVLLVLRGGAALLCLPCDESKCEEPKQCTGAVVLGICGCCSVCAKQKNESCGGVYGLYGTCDAGLRCVIRPPLDGASITQYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIIDGKCECDSVRTCNNPFEFASQEACQTALRKIEEERPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPAGCLRKVCQPGYLNILVSKSSGKPGECCDLYECKPAPVLPVQCPADSYETQVRLTADGCCTLPTRCECLPSVCTFPQCSAGTSPHLVSRGNGTPGRCCDVFECVNETKPACTFNGVEYHDGDMFRMDTCRFCRCQGGVSVCFTAQCGVLHCDRYYVPEGECCPVCEDPIFPALNFAGCYVNGQILAHGDHWREDDCTFCQCVSGESRCVAAACGHSCLNPVTVPGECCPVCEEPTYITMAPPACGPLENCTLTVQDCLYGFRLDQDGCRTCSCKTREELCRGLISGCTLKCPFGFQTDAHGCTVCQCRQRHKKCKPVACAKDCPFGFVKNKHGCDTCRCKKCPELPCDKPCPMGFQQDELGCLICQCRDQSSSSVGPSVKLGSCLSMDGRRHENGQSWHDGCRECFCHAGREMCALISCPVPSCDNPSIRAGQCCPTCPDESSSHKPELSEASVCLAPGGEYFVEGETWNIDSCTQCTCHVGRVLCETEVCPPLLCQSPIRTQDSCCPHCPDEPVTPPATSNVSMPSYCRNEEGDIFLVAESWKPNVCSSCVCLDGVISCFSESCPPVNCARPVLRKGQCCPYCLDATPRAVCHFNGKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPALPCSQPITVEGSCCSMCPEIAPTNVPIEKTDQHGDRLRHRPAWPTHSENDIMPQFRGDFGSLQMPYLDGKTPLPSEDPSLHWAWVALPILMMVLTIATLLLINQRKQWIPVPCYHTPTKPTCLNNQLVYVDCQKGTKVQVNSSQRMLRIADPDSRYSGYYSMPKHNNLQADNFYQTA

Target ORF information:

RefSeq Version	XM_007252565.2
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007252565.2

ORF Insert Sequence:

ATGCACCCCT TGGTGAACCG CGTGCTGTTC CCGCAGCTGG CCGTGCTGCT GGTGCTGCGC 
GGCGGCGCGG CGCTGCTCTG CCTGCCCTGC GACGAGTCCA AGTGCGAGGA GCCCAAGCAG 
TGCACGGGCG CCGTGGTGCT GGGCATCTGC GGCTGCTGCT CCGTGTGCGC CAAGCAGAAG 
AACGAGAGCT GCGGCGGCGT GTACGGGCTG TACGGGACGT GCGACGCGGG GCTGCGCTGC 
GTCATCCGCC CTCCGCTCGA CGGAGCCTCG ATCACCCAGT ACGAGGTCGG AGTTTGCGAA 
GATGAAAACT GGGATGACGA CCAGCTCTTG GGATTTGAGC CGTGCAATGA GAACCTGGTT 
ACGGGCTGCA ACATCATCGA TGGGAAGTGT GAATGTGACA GCGTACGGAC GTGCAACAAC 
CCATTTGAGT TTGCCAGCCA GGAAGCCTGT CAGACAGCCC TGAGGAAGAT CGAAGAGGAG 
AGACCGGACT GTTCTAAGGC CAGGTGTGAA GTTCAGTTTT CTCCTCGGTG TCCGGAGGAC 
TCTGTGCTGA TCGAGGGTTA CGCCCCACCG GGGGAGTGCT GCCCCCTGCC CAGCCGCTGT 
GTGTGCAGCC CTGCGGGTTG TTTGAGAAAA GTGTGCCAGC CAGGCTACCT CAACATCCTG 
GTGTCCAAAA GCTCGGGCAA GCCTGGAGAG TGCTGTGACC TGTATGAGTG CAAACCAGCA 
CCCGTCCTGC CGGTCCAGTG TCCAGCTGAC AGCTACGAGA CCCAGGTTAG ACTGACAGCA 
GATGGTTGCT GCACCCTTCC TACAAGGTGC GAGTGTTTGC CCAGCGTCTG CACCTTCCCG 
CAGTGTTCGG CTGGAACGTC CCCTCACTTG GTCTCCCGTG GCAACGGAAC ACCAGGGCGG 
TGCTGTGATG TGTTTGAGTG TGTTAATGAG ACCAAGCCAG CCTGTACCTT CAACGGTGTG 
GAGTACCATG ATGGAGACAT GTTCAGGATG GACACCTGCC GCTTCTGCCG CTGCCAGGGC 
GGAGTGTCTG TGTGCTTCAC AGCGCAGTGC GGGGTCCTCC ACTGCGACCG CTACTACGTA 
CCTGAGGGGG AGTGCTGCCC TGTCTGTGAA GATCCCATCT TTCCAGCTCT GAACTTTGCA 
GGCTGCTATG TGAACGGTCA GATCCTGGCT CACGGTGACC ACTGGCGTGA GGATGACTGC 
ACGTTCTGCC AGTGTGTGAG CGGGGAGTCT CGTTGCGTGG CGGCTGCCTG CGGCCATAGC 
TGCCTCAACC CCGTCACGGT GCCCGGAGAA TGCTGCCCTG TGTGTGAGGA GCCCACCTAC 
ATCACCATGG CGCCCCCTGC CTGTGGCCCG CTGGAGAACT GCACGCTGAC GGTCCAGGAC 
TGCCTGTATG GTTTCCGGCT CGACCAGGAC GGCTGCCGCA CCTGCAGCTG CAAAACAAGA 
GAGGAGCTGT GCAGAGGCCT GATCTCCGGC TGCACGCTGA AGTGCCCGTT TGGTTTTCAG 
ACGGATGCTC ATGGCTGCAC CGTGTGCCAG TGCCGACAGC GACACAAGAA GTGCAAGCCG 
GTGGCCTGTG CCAAGGACTG TCCATTTGGC TTTGTCAAAA ACAAGCACGG CTGTGACACC 
TGTCGCTGTA AGAAGTGCCC CGAGCTGCCG TGTGACAAGC CGTGTCCAAT GGGCTTCCAG 
CAGGACGAGC TGGGCTGCCT GATATGCCAG TGTCGAGACC AGAGCTCCTC TTCTGTGGGC 
CCTTCGGTTA AGCTGGGATC GTGCCTCTCT ATGGATGGAC GCAGACATGA GAATGGTCAG 
AGTTGGCACG ACGGCTGCAG GGAGTGTTTT TGTCATGCCG GTCGAGAGAT GTGTGCACTT 
ATATCCTGCC CAGTGCCTTC CTGTGACAAC CCCAGCATCC GCGCCGGCCA GTGCTGCCCC 
ACCTGTCCAG ACGAGTCCAG CTCTCACAAG CCGGAGCTGA GTGAAGCGTC TGTGTGCTTG 
GCACCAGGTG GGGAGTATTT TGTGGAGGGC GAGACGTGGA ACATCGACTC CTGCACGCAG 
TGCACCTGCC ACGTCGGCCG CGTGCTCTGC GAGACCGAGG TGTGTCCGCC TCTGCTCTGC 
CAGAGCCCTA TCAGAACACA GGACTCCTGC TGCCCACACT GCCCAGATGA GCCGGTCACC 
CCTCCGGCCA CCAGCAACGT CAGCATGCCC AGCTACTGCC GCAACGAGGA GGGCGACATC 
TTCCTGGTGG CCGAGTCCTG GAAGCCCAAC GTCTGCTCCA GCTGTGTGTG TTTGGACGGA 
GTAATCAGCT GCTTCTCTGA ATCCTGCCCT CCGGTCAACT GCGCCCGGCC TGTCCTCCGG 
AAGGGCCAGT GCTGCCCCTA CTGCTTAGAT GCCACGCCAA GGGCCGTGTG CCACTTCAAC 
GGCAAGACCT ACGTGGACGA GGAGCGCTGG GACATCGACA GCTGCACGCA CTGCTACTGC 
CTGCAGGGTC AGACGCTCTG CTCCACCGTC AGCTGCCCTG CCCTGCCCTG CAGCCAACCC 
ATCACAGTGG AGGGCAGCTG CTGCTCCATG TGCCCAGAGA TCGCCCCCAC TAATGTTCCC 
ATAGAGAAGA CAGACCAACA TGGAGACAGA CTGAGGCATC GGCCGGCCTG GCCTACACAC 
AGCGAGAATG ACATAATGCC CCAGTTCAGA GGTGATTTCG GCAGCCTGCA GATGCCATAT 
CTGGATGGGA AGACGCCATT GCCCAGTGAG GATCCCAGTC TTCACTGGGC CTGGGTGGCT 
CTGCCCATCC TCATGATGGT GCTCACTATC GCCACCCTGC TGCTGATCAA TCAGAGAAAG 
CAGTGGATCC CAGTGCCTTG CTATCACACT CCCACTAAGC CCACGTGTCT GAATAACCAG 
CTGGTGTATG TGGACTGCCA GAAGGGCACA AAAGTTCAGG TGAACAGCTC TCAGCGCATG 
CTCCGCATCG CAGACCCCGA CTCGCGCTAC AGCGGCTACT ACAGCATGCC CAAACACAAC 
AACCTGCAAG CGGACAACTT CTACCAGACT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_022662672.1 ) cDNA ORF clone, Astyanax mexicanus(Mexican tetra) -> XP_022518393.1 Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Price & Availability↑

CloneID	OAs97534
Clone ID Related Accession (Same CDS sequence)	XM_022662672.1
Accession Version	XM_022662672.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Astyanax mexicanus(Mexican tetra)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019171711.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Astyanax mexicanus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCCCC TGGTGAACCG CGTGCTGTTC CCGCAGCTGG CCGTGCTGCT GCTGCTGGTG 
CTGCGCGGCG GCGCGGCGCT GCTCTGCCTG CCCTGCGACG AGTCCAAGTG CGAGGAGCCC 
AAGCAGTGCA CGGGCGCCGT GGTGCTGGGC ATCTGCGGCT GCTGCTCCGT GTGCGCCAAG 
CAGAAGAACG AGAGCTGCGG CGGCGTGTAC GGGCTGTACG GGACGTGCGA CGTGGGGCTG 
CGCTGCGTCA TCCGCCCTCC GCTCGACGGA GCCTCGATCA CCCAGTACGA GGTCGGAGTT 
TGCGAAGATG AAAACTGGGA TGACGACCAG CTCTTGGGAT TTGAGCCGTG CAATGAGAAC 
CTGGTTACGG GCTGCAACAT CATCGATGGG AAGTGTGAAT GTGACAGCGT ACGGACGTGC 
AACAACCCAT TTGAGTTTGC CAGCCAGGAA GCCTGTCAGA CAGCCCTGAG GAAGATCGAA 
GAGGAGAAAC CGGACTGTTC TAAGGCCAGG TGTGAAGTTC AGTTTTCTCC TCGGTGTCCG 
GAGGACTCTG TGCTGATCGA GGGTTACGCC CCACCGGGGG AGTGCTGCCC CCTGCCCAGC 
CGCTGTGTGT GCAGCCCTGC GGGTTGTTTG AGAAAAGTGT GCCAGCCAGG CTACCTCAAC 
ATCCTGGTGT CCAAAAGCTC GGGCAAGCCT GGAGAGTGCT GTGACCTGTA TGAGTGCAAA 
CCAGTTTTCA GTGTGGACTG CAGCACAGTA GAATGTCCAC CCGTCCTGCC GGTCCAGTGT 
CCAGCTGACA GCTACGAGAC CCAGGTTAGA CTGACAGCAG ATGGTTGCTG CACCCTTCCT 
ACAAGGTGCG AGTGTTTGCC CAGCGTCTGC ACCTTCCCGC AGTGTTCGGC TGGAACGTCC 
CCTCACTTGG TCTCCCGTGG CAACGGAACA CCAGGGCGGT GCTGTGATGT GTTTGAGTGT 
GTTAATGAGA CCAAGCCAGC CTGTACCTTC AACGGTGTGG AGTACCATGA TGGAGACATG 
TTCAGGATGG ACACCTGCCG CTTCTGCCGC TGCCAGGGCG GAGTGTCTGT GTGCTTCACA 
GCGCAGTGCG GGGTCCTCCA CTGCGACCGC TACTACGTAC CTGAGGGGGA GTGCTGCCCT 
GTCTGTGAAG ATCCCATCTT TCCAGCTCTG AACTTTGCAG GCTGCTATGT GAACGGTCAG 
ATCCTGGCTC ACGGTGACCA CTGGCGTGAG GATGACTGCA CGTTCTGCCA GTGTGTGAGC 
GGGGAGTCTC GTTGCGTGGC GGCTGCCTGC GGCCATAGCT GCCTCAACCC CGTCACGGTG 
CCCGGAGAAT GCTGCCCTGT GTGTGAGGAG CCCACCTACA TCACCATGGC GCCCCCTGCC 
TGTGGCCCGC TGGAGAACTG CACGCTGACG GTCCAGGACT GCCTGTACGG TTTCCGGCTC 
GACCAGGACG GCTGCCGCAC CTGCAGCTGC AAAACAAGAG AGGAGCTGTG CAGAGGCCTG 
ATCTCCGGCT GCACGCTGAA GTGCCCGTTT GGTTTTCAGA CGGATGCTCA TGGCTGCACC 
GTGTGCCAGT GCCGACAGCG ACACAAGAAG TGCAAGCCGG TGGCCTGTGC CAAGGACTGT 
CCATTTGGCT TTGTCAAAAA CAAGCACGGC TGTGACACCT GTCGCTGTAA GAAGTGCCCC 
GAGCTGCCAT GTGACAAGCC GTGTCCAATG GGCTTCCAGC AGGACGAGCT GGGCTGCCTG 
ATATGCCAGT GTCGAGACCA GAGCTCCTCT TCTGTGGGCC CTTCGGTTAA GCTGGGATCG 
TGCCTCTCTA TGGATGGACG CAGACATGAG AATGGTCAGA GTTGGCACGA CGGCTGCAGG 
GAGTGTTTTT GTCATGCCGG TCGAGAGATG TGTGCACTTA TATCCTGCCC AGTGCCTTCC 
TGTGACAACC CCAGCATCCG CGCCGGCCAG TGCTGCCCCA CCTGTCCAGA CGAGTCCAGC 
TCTCACAAGC CGGAGCTGAG TGAAGCGTCT GTGTGCTTGG CACCGGGTGG GGAGTATTTT 
GTGGAGGGCG AGACGTGGAA CATCGACTCC TGCACGCAGT GCACCTGCCA CGTCGGCCGC 
GTGCTCTGCG AGACCGAGGT GTGTCCGCCT CTGCTCTGCC AGAGCCCTAT CAGAACGCAG 
GACTCCTGCT GCCCACACTG CCCAGATGAG CCGGTCACCC CTCCGGCCAC CAGCAACGTC 
AGCATGCCCA GCTACTGCCG CAACGAGGAG GGCGACATCT TCCTGGTGGC CGAGTCCTGG 
AAGCCCAACG TCTGCTCCAG CTGTGTGTGT TTGGACGGAG TAATCAGCTG CTTCTCTGAA 
TCCTGCCCTC CGGTCAACTG CGCCCGGCCT GTCCTCCGGA AGGGCCAGTG CTGCCCCTAC 
TGCTTAGATG CCACGCCAAG GGCCGTGTGC CACTTCAACG GCAAGACCTA CGTGGACGAG 
GAGCGCTGGG ACATCGACAG CTGCACGCAC TGCTACTGCC TGCAGGGTCA GACGCTCTGC 
TCCACCGTCA GCTGCCCTGC CCTGCCCTGC AGCCAACCCA TCACAGTGGA GGGCAGCTGC 
TGCTCCATGT GCCCAGAGAT CGCCCCCACT AATGTTCCCA TAGAGAAGAC AGACCAACAT 
GGAGACAGAC TGAGGCATCG GCCGGCCTGG CCTACACACA GCGAGAATGA CATAATGCCC 
CAGTTCAGAG GTGATTTCGG CAGCCTGCAG ATGCCATATC TGGATGGGAA GACACCATTG 
CCCAGTGAGG ATCCCAGTCT TCACTGGGCC TGGGTGGCTC TGCCCATCCT CATGATGGTG 
CTCACTATCG CCACCCTGCT GCTGATCAAT CAGAGAAAGC AGTGGATCCC AGTGCCTTGC 
TATCACACTC CCACTAAGCC CACGTGTCTG AATAACCAGC TGGTGTATGT GGACTGCCAG 
AAGGGCACAA AAGTTCAGGT GAACAGCTCT CAGCGCATGC TCCGCATCGC AGACCCCGAC 
TCGCGCTACA GCGGCTACTA CAGCATGCCC AAACACAACA ACCTGCAAGC GGACAACTTC 
TACCAGACTG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022518393.1
CDS	186..3260
Translation	MHPLVNRVLFPQLAVLLLLVLRGGAALLCLPCDESKCEEPKQCTGAVVLGICGCCSVCAKQKNESCGGVYGLYGTCDVGLRCVIRPPLDGASITQYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIIDGKCECDSVRTCNNPFEFASQEACQTALRKIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPAGCLRKVCQPGYLNILVSKSSGKPGECCDLYECKPVFSVDCSTVECPPVLPVQCPADSYETQVRLTADGCCTLPTRCECLPSVCTFPQCSAGTSPHLVSRGNGTPGRCCDVFECVNETKPACTFNGVEYHDGDMFRMDTCRFCRCQGGVSVCFTAQCGVLHCDRYYVPEGECCPVCEDPIFPALNFAGCYVNGQILAHGDHWREDDCTFCQCVSGESRCVAAACGHSCLNPVTVPGECCPVCEEPTYITMAPPACGPLENCTLTVQDCLYGFRLDQDGCRTCSCKTREELCRGLISGCTLKCPFGFQTDAHGCTVCQCRQRHKKCKPVACAKDCPFGFVKNKHGCDTCRCKKCPELPCDKPCPMGFQQDELGCLICQCRDQSSSSVGPSVKLGSCLSMDGRRHENGQSWHDGCRECFCHAGREMCALISCPVPSCDNPSIRAGQCCPTCPDESSSHKPELSEASVCLAPGGEYFVEGETWNIDSCTQCTCHVGRVLCETEVCPPLLCQSPIRTQDSCCPHCPDEPVTPPATSNVSMPSYCRNEEGDIFLVAESWKPNVCSSCVCLDGVISCFSESCPPVNCARPVLRKGQCCPYCLDATPRAVCHFNGKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPALPCSQPITVEGSCCSMCPEIAPTNVPIEKTDQHGDRLRHRPAWPTHSENDIMPQFRGDFGSLQMPYLDGKTPLPSEDPSLHWAWVALPILMMVLTIATLLLINQRKQWIPVPCYHTPTKPTCLNNQLVYVDCQKGTKVQVNSSQRMLRIADPDSRYSGYYSMPKHNNLQADNFYQTA

Target ORF information:

RefSeq Version	XM_022662672.1
Organism	Astyanax mexicanus(Mexican tetra)
Definition	Astyanax mexicanus cysteine rich transmembrane BMP regulator 1 (crim1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022662672.1

ORF Insert Sequence:

ATGCACCCCC TGGTGAACCG CGTGCTGTTC CCGCAGCTGG CCGTGCTGCT GCTGCTGGTG 
CTGCGCGGCG GCGCGGCGCT GCTCTGCCTG CCCTGCGACG AGTCCAAGTG CGAGGAGCCC 
AAGCAGTGCA CGGGCGCCGT GGTGCTGGGC ATCTGCGGCT GCTGCTCCGT GTGCGCCAAG 
CAGAAGAACG AGAGCTGCGG CGGCGTGTAC GGGCTGTACG GGACGTGCGA CGTGGGGCTG 
CGCTGCGTCA TCCGCCCTCC GCTCGACGGA GCCTCGATCA CCCAGTACGA GGTCGGAGTT 
TGCGAAGATG AAAACTGGGA TGACGACCAG CTCTTGGGAT TTGAGCCGTG CAATGAGAAC 
CTGGTTACGG GCTGCAACAT CATCGATGGG AAGTGTGAAT GTGACAGCGT ACGGACGTGC 
AACAACCCAT TTGAGTTTGC CAGCCAGGAA GCCTGTCAGA CAGCCCTGAG GAAGATCGAA 
GAGGAGAAAC CGGACTGTTC TAAGGCCAGG TGTGAAGTTC AGTTTTCTCC TCGGTGTCCG 
GAGGACTCTG TGCTGATCGA GGGTTACGCC CCACCGGGGG AGTGCTGCCC CCTGCCCAGC 
CGCTGTGTGT GCAGCCCTGC GGGTTGTTTG AGAAAAGTGT GCCAGCCAGG CTACCTCAAC 
ATCCTGGTGT CCAAAAGCTC GGGCAAGCCT GGAGAGTGCT GTGACCTGTA TGAGTGCAAA 
CCAGTTTTCA GTGTGGACTG CAGCACAGTA GAATGTCCAC CCGTCCTGCC GGTCCAGTGT 
CCAGCTGACA GCTACGAGAC CCAGGTTAGA CTGACAGCAG ATGGTTGCTG CACCCTTCCT 
ACAAGGTGCG AGTGTTTGCC CAGCGTCTGC ACCTTCCCGC AGTGTTCGGC TGGAACGTCC 
CCTCACTTGG TCTCCCGTGG CAACGGAACA CCAGGGCGGT GCTGTGATGT GTTTGAGTGT 
GTTAATGAGA CCAAGCCAGC CTGTACCTTC AACGGTGTGG AGTACCATGA TGGAGACATG 
TTCAGGATGG ACACCTGCCG CTTCTGCCGC TGCCAGGGCG GAGTGTCTGT GTGCTTCACA 
GCGCAGTGCG GGGTCCTCCA CTGCGACCGC TACTACGTAC CTGAGGGGGA GTGCTGCCCT 
GTCTGTGAAG ATCCCATCTT TCCAGCTCTG AACTTTGCAG GCTGCTATGT GAACGGTCAG 
ATCCTGGCTC ACGGTGACCA CTGGCGTGAG GATGACTGCA CGTTCTGCCA GTGTGTGAGC 
GGGGAGTCTC GTTGCGTGGC GGCTGCCTGC GGCCATAGCT GCCTCAACCC CGTCACGGTG 
CCCGGAGAAT GCTGCCCTGT GTGTGAGGAG CCCACCTACA TCACCATGGC GCCCCCTGCC 
TGTGGCCCGC TGGAGAACTG CACGCTGACG GTCCAGGACT GCCTGTACGG TTTCCGGCTC 
GACCAGGACG GCTGCCGCAC CTGCAGCTGC AAAACAAGAG AGGAGCTGTG CAGAGGCCTG 
ATCTCCGGCT GCACGCTGAA GTGCCCGTTT GGTTTTCAGA CGGATGCTCA TGGCTGCACC 
GTGTGCCAGT GCCGACAGCG ACACAAGAAG TGCAAGCCGG TGGCCTGTGC CAAGGACTGT 
CCATTTGGCT TTGTCAAAAA CAAGCACGGC TGTGACACCT GTCGCTGTAA GAAGTGCCCC 
GAGCTGCCAT GTGACAAGCC GTGTCCAATG GGCTTCCAGC AGGACGAGCT GGGCTGCCTG 
ATATGCCAGT GTCGAGACCA GAGCTCCTCT TCTGTGGGCC CTTCGGTTAA GCTGGGATCG 
TGCCTCTCTA TGGATGGACG CAGACATGAG AATGGTCAGA GTTGGCACGA CGGCTGCAGG 
GAGTGTTTTT GTCATGCCGG TCGAGAGATG TGTGCACTTA TATCCTGCCC AGTGCCTTCC 
TGTGACAACC CCAGCATCCG CGCCGGCCAG TGCTGCCCCA CCTGTCCAGA CGAGTCCAGC 
TCTCACAAGC CGGAGCTGAG TGAAGCGTCT GTGTGCTTGG CACCGGGTGG GGAGTATTTT 
GTGGAGGGCG AGACGTGGAA CATCGACTCC TGCACGCAGT GCACCTGCCA CGTCGGCCGC 
GTGCTCTGCG AGACCGAGGT GTGTCCGCCT CTGCTCTGCC AGAGCCCTAT CAGAACGCAG 
GACTCCTGCT GCCCACACTG CCCAGATGAG CCGGTCACCC CTCCGGCCAC CAGCAACGTC 
AGCATGCCCA GCTACTGCCG CAACGAGGAG GGCGACATCT TCCTGGTGGC CGAGTCCTGG 
AAGCCCAACG TCTGCTCCAG CTGTGTGTGT TTGGACGGAG TAATCAGCTG CTTCTCTGAA 
TCCTGCCCTC CGGTCAACTG CGCCCGGCCT GTCCTCCGGA AGGGCCAGTG CTGCCCCTAC 
TGCTTAGATG CCACGCCAAG GGCCGTGTGC CACTTCAACG GCAAGACCTA CGTGGACGAG 
GAGCGCTGGG ACATCGACAG CTGCACGCAC TGCTACTGCC TGCAGGGTCA GACGCTCTGC 
TCCACCGTCA GCTGCCCTGC CCTGCCCTGC AGCCAACCCA TCACAGTGGA GGGCAGCTGC 
TGCTCCATGT GCCCAGAGAT CGCCCCCACT AATGTTCCCA TAGAGAAGAC AGACCAACAT 
GGAGACAGAC TGAGGCATCG GCCGGCCTGG CCTACACACA GCGAGAATGA CATAATGCCC 
CAGTTCAGAG GTGATTTCGG CAGCCTGCAG ATGCCATATC TGGATGGGAA GACACCATTG 
CCCAGTGAGG ATCCCAGTCT TCACTGGGCC TGGGTGGCTC TGCCCATCCT CATGATGGTG 
CTCACTATCG CCACCCTGCT GCTGATCAAT CAGAGAAAGC AGTGGATCCC AGTGCCTTGC 
TATCACACTC CCACTAAGCC CACGTGTCTG AATAACCAGC TGGTGTATGT GGACTGCCAG 
AAGGGCACAA AAGTTCAGGT GAACAGCTCT CAGCGCATGC TCCGCATCGC AGACCCCGAC 
TCGCGCTACA GCGGCTACTA CAGCATGCCC AAACACAACA ACCTGCAAGC GGACAACTTC 
TACCAGACTG CGTGA