NFIA_083650 cDNA ORF clone, Aspergillus fischeri NRRL 181(Neosartorya fischeri NRRL 181)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFIA_083650
Entrez Gene ID	4586868
Full Name	translational activator GCN1
Gene Type	protein-coding
Organism	Aspergillus fischeri NRRL 181(Neosartorya fischeri NRRL 181)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001260309.1	XP_001260310.1	translational activator GCN1

Pathways from BioSystems
Gene Ontology

The following NFIA_083650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFIA_083650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe10324	XM_001260309.1 Latest version!	Aspergillus fischeri NRRL 181 translational activator GCN1 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFIA_083650 ( XM_001260309.1 ) cDNA ORF clone, Aspergillus fischeri NRRL 181(Neosartorya fischeri NRRL 181) -> XP_001260310.1 Aspergillus fischeri NRRL 181 translational activator GCN1 partial mRNA.

Price & Availability↑

CloneID	ONe10324
Clone ID Related Accession (Same CDS sequence)	XM_001260309.1
Accession Version	XM_001260309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8022bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-11
Organism	Aspergillus fischeri NRRL 181(Neosartorya fischeri NRRL 181)
Product	translational activator GCN1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_001509762). COMPLETENESS: incomplete on both ends.

ATGGACGACT TTCCATGGGA ACAGGTGAAA TCAGGAGACC TGGAGAGCCT GCGAGACGCT 
CTCTTTTCGA GCTCGACTAC TCGCAGAACT CATGCTCTCC AGGAGCTCCG CGATAGAGTT 
GGCTCTGAAT TACCCCAAGA AATCCGTCAG TCTCTTATAG GACTCCTTTT TACGACCTAT 
CCGCTCTACG TCGATCGACC CTCTCGACAA GCTGTCCAGC AATGTCTGCG TACGATCCTC 
AAGGCGCCCG TTCCGACCGA AGATCTGAAA TACCTTACCC AGAATCTGCT CGCAGAGGCT 
TCTAAACCCG GATTGGCTCC TTCTTCCGCG TTTGTCCTGC TGGAATGGTG CTCCCTTCTG 
CTGCAGATAT TGAAAAATGA CCCGGACACC CCTCTCTCGA TCGTCCTGGA CATTGTCGCT 
GTGGATGCAA AGGCTCTCGA AACCTGCCTT GCTGCTCGTC CCAGACCAAC GGTGAAACAG 
TCTGCCCTTA CTGTTACGAG ACGTGCTCTA CGCGCCGTTT TCTCGTCAGA CAATTGGGGC 
GAGGATGCTG TCCGCCAGTC AGTCGCTCGG CTGACTGGTG ACTCGGCGGG GCAGAAGAAT 
GCGCCCTTCC TCGGTGTCGT CTCTGGTGTA TGCGCCCACT TACCGAACAG GAAGGCCGTC 
CTGGAGAATG AAAAGAAATT GATCCTCGCA TACTATGTCA AAGAATTGAT CGGCTCAAAA 
GCTGCAGTGC CAAACCACAT CGCAAGTGGC TTATCTGACT TCTTCGCCTC TTTTATTACC 
TATGAGGATT TCACGTCGGA GCTAGTACCT CCTCTTGAGA AATCGCTCTT GCGTGCTCCA 
GAGGTGGTAT TGGGTGGATT GGTGCCTTCA CTATGCTCTT CTCTACCAGA GGACTTTGAC 
CTGTCCGAGA TTCTGTTCTC TCGGCTCCTG AAGCACCTTC TCTCCAGCAT GAAATCAAAC 
AATGCGACTA TTAGGCAAGG TGCTGTGCAG TCTCTTGAGT ACATCCTCTC AAAGTCTAAA 
AATGAGGATT GGCTTGTCAA GATCGCTGGT GAGATCATCG GGCCCCTAAA GACCCAGAAG 
ATCACTAGCC CAGAACATCG TGCTGTGTAT GCTCAAGCGG TCTGTGCTAT CCTGCCTAGT 
GGGAGTGTCT CCAAAGACAT AGTGCAAGGG TTTGCTCCTG TCTTCTCGCG GGAATCTAAC 
GAAGTAGCAT TGGAACAAGA AATCAAGGCC CTCTGCAAGC ACCTCACTTT CCTCCTTCAA 
ACCAAGGTGA AAGTTAGTGA CGATGTCATC AATGCCGTCG TCAAAGGAAT TGCAGATAAG 
CGTATCCCGT TCAGGAAGCT TTGGCAACTG ATTGTTGGAG ACGTCTTCTG GCGTATTGAA 
GCACCAACAC TGGCTACCTC CGAGGTTGGA CCATTTGTCA CCAAGTTCCT CGACAAGATG 
AAGGATTTGT TCAAGGAGGT AACTTCAAAT CCTCTACCTT CAGCTCAGAA TGGCTCTTTA 
TCCGCAGCAT TTGTATACCT TGCCCTCTAC GAAAGGGTAT CTACAGCCCA GGGATTAGAT 
AAGGCGGACT GGGAAACCAC TGTGGCACAG TCGATGCTCC TCACCCCAAA GCCTTCTTTT 
CTTCTGAACC CTAAGGCTTA TACCAAATTG ACGTCTCCAG CAGAGATCCA GTGGATGGTT 
CGCGCCCTTG TGGCTGTCTC GTCTGGATCC AAGTTCGAGA GCACCGAAGA TGCAGCCAAG 
GTTGCATGGG CTCAGGCATT CATTTACGCT GTCACGGCTC CCGGACTTCG CACAAACTTG 
CGTGAGGAAG CTGCTCACGC TCTTATGGGG ATTTATCTGC GCAGGTCGGA ACTGATGGGT 
CGCGTGGTCA CTGAAGCTCT GTGGACGTGG ATTCTCGCCT TGAGAACGGC GGAGAAAGAT 
TCTGCTGCAT CGTCTGCAGG ACCGGCGAGT GAGAAGCTCC TTCACCTCGT AGCGAAGGCC 
ATCTGTCCTT CTGCCGCCGA TATACAGTCC GATAAGATTC GATCTGATCT GAAGCGTCAA 
CTCGTTCAGC TTCTTGTACT TTGCAGGCCA GAACTGATTC CCAATATTTC ATGGATCGCA 
CTGTGCTTAA GGACTGGTAC AGATCCTGGT GATCTTGTCC GTGAATATCC GGATGATTTT 
ATGAAGCAAT TGACGCAGGT GCATGAGGAC CCCATCCAAT CGAAAGTCCC TGCGATTGAT 
GTAGCTATCT GGAATGCTGC AGGGGACCTG GCATTTGTCG CACCAGACAC TATGGTCCCT 
CGCCTGATTC GGCAGATCAG AGACGATTTG GATGCATCGC GTCTTTCTAG GTTCACACCA 
ACCGATGCAG CCATTGCCCG CACTCCAGAG GGGACCATGT TCGTTGATGT CCTCAACACC 
AAGTCGATGC AGCCAGCATT CGACAAGAAC ACCAAAGACT ACGACACCCT CAAATGGGAA 
GAAGAGCTGC GGGCGCAATT AGCTCAGAAG AAAGGTCAAA CGCAGAAGAA GCTTAGCGCG 
GATGAACAAG CGAAGGTCAA AGCTCAACTT GCCAAGGAAT CTAAGATCCG TGAGGAGGTC 
CTACAGGAAG TGAAGAGAGT CGAAAGAGGC GCCGGCATTA TTCAGGGGCT TGCGAGCGGG 
CCCGCCGTTG ATGCTGATGG ATGGATCAAC CCGGCTGTAA GCTCATTGCT GTCTCTGGCC 
CGAGCTGGCG TGGGCTTGTT CGCGGGTGAC GTCGTTTCCA AAGCGTACGT GAAATGCGCT 
GAGAAAGTCT CCTCGCGCCT AGGTCCTCTT CGCCCCTTCG TTGGTGTTGC GACACTACGA 
GCAATTGGCA GGTCCCATCT GCCGCCTGAG ATGGAAGCCG AGCCACTCGG TGGGCTTGTT 
ACAAGAATTC TTTATCGCCT TCGTTTTGCG TCTGAGCAGC GCCCATTCGA CATGACATCC 
TTGGCATATA TACTTCCCTT GATATTCATG GTTCTGAGCC GCAATGGCAT CGAAGAAGTC 
AAAGGGGAAG AAGAAGGAGA GCAGTTGCTT CTGGCGCTGG AGTTCCTGTC ATTCCATTCC 
GGTTCATTCA CTGACAACCG TCTTCCACGC GTTGAGGTTC TGAATCATCT GCTTACTGCC 
ATGCAGAAGT TTACCCAGCA CTACAAGCTG ATCAAGGATA CTCTCTTCGA TTTCTGTAGA 
TGCATTTCGT CCAACATCAC CAATGAGGAG CTTAATGTCC TTCTACAAGG CACCATCATT 
CCAGATACAT CCGTCCGAAC CTCGGTGCTC CAGGTTATTG AGTCTGAAAT TGATCTGACT 
GACCTGGACT TCTCTGAGCA CATCTGGTTG GGCTGTCACG ATCAGGTGGA GGAGAACGCG 
GAAATTGCTG AAAATATCTG GGAGGAAAAT GCCCTCGAGG TCGACGAGAC TTCCTACGGG 
AAAATAATCC CATACCTCTC TTCCAAGGAC TCCCAACTCC GAGGCGCTGC TGCCCGCGCG 
TTGGGCCATG CAGTGGAGCT CAATCCGTCG GTTTTCGGGG ACATTGTCCA GCAATTGCAG 
TCAATGTACG AGGAGGAAGT GAAGCCCAAA GAGCCTGCGA AGGACAAGTA CGGAATGCCG 
CTCAAGGTGG ATACGACCGA CCACTGGGAA TGCCGCAGTG GCATTGCCCT CGCTTTTGGT 
GCGATGACAA ATAGTTTCGA AGGCGATCAG ATTGTTTCGT TCCTTCGCTT TCTGATTGAA 
AGAGGACCCT TGATTGACAG AAACTCTATG GTGAGATCCC AAATGGCTGA CAGCGGCAGA 
TCCGTCGTCG CGTCGCGCGG CCAGCAGCGG GTTGAGGAGT TGATGGAAAT ACTGGAAACC 
ACTCTGGAAA CTTCAGACAA AGGAAGCGAG ACATCCGATC TTCTCAATGA AGCAGTGGTT 
GTTCTCTACG GTTCTCTGGC TCAGCATCTC AAGTCGGACG ATCCGCGGTT GCAAACAGTA 
ATCAAGCGAC TTCTGGCGAC ACTCCCGACT CCGTCGGAAA CTGTACAATC GGCCGTCTCG 
GGATGCCTTC CACCACTCAT TCGTCTCTGC GGGCCAAAGA GCGGAGAGTA TGTACAAGAG 
ATGCTGGATC AACTGTTGCA GACCAAGAAC TATGCTACCC AGCGTGGAGC GGCTTACGGT 
CTGGCTGGTA TCGTTCGTGG AAGGGGCATC TTCACTCTGC GGGAGTACCA GGTCATGTCT 
CACCTGAAGG ACGCAACTGA GAATAAGAAA GAGGCACATC AGCGACTGGG TGCTCTGTTA 
GCGTTTGAAC TCTTCGCCAC CATCCTCGGG CGCACATTTG AACCATATGT CATTCAGATC 
GTGCCGCAGC TCCTTGCCGG TTTCGGTGAC CCTAGCATCG ACGTCCGCGA TGCTTGCCTC 
GACGCAGCAA AGGCTTGCTT CTCTAATCTC AGCTCCTACG GTGTGAAGAA GATTCTCCCT 
ACTCTCCTCG ATGGTCTTGA CGACACGCAA TGGCGCAGTC AAAAGGGAGC CTGCGATCTC 
CTTGGAGCAA TGGCTTATCT CGACCCCCAG CAATTGGCAG CAAGCTTGCC CGACATCATC 
CCTCCACTTA CCGTGGTGCT CAATGATACT CACAAGGAGG TGCGCAACGC CGCAAATCGC 
AGTCTGCAGC GATTCGGTGA GGTCATCAGC AACCCCGAAG TGAAGGGCTT GGTCAATGTC 
CTTCTCAAGG CCCTGAGTGA TCCGACCAAA TACACAGATG AGGCTCTGGA TGCACTTATC 
AAGGTCTCTT TCGTTCATTA TTTGGATGCT CCCTCTTTGG CGCTTGTTGT CCGTATCCTT 
GAGCGCGGTC TTGGCGACAG ATCAAATACC AAGAGGAAGT CCGCACAGAT CATTGGCAGC 
TTGGCTCACC TTACTGAGCG GAAGGATCTT ATCTCTCACC TGCCCATTAT CGTTTCTGGA 
TTGCACTTAG CCATCGTCGA TCCAGTGCCT ACTACCCGTG CCACCGCGTC CAAAGCTCTC 
GGCTCACTTA TCGAGAAGCT CGGAGAGGAT GCACTACCCG ATCTCATTCC CAACCTCATG 
ACTACGCTCA AGTCGGATAC TGGCGCCGGC GATCGTTTGG GATCCGCACA GGCTCTTGCG 
GAGGTTCTGG CAGGTCTGGG AACGACAAGA CTGGAAGAGA CACTGCCAAC AATCTTGCAG 
AACGTCTCAA GCTCCAAGCC GGCTGTTCGG GAAGGCTTCA TGACGCTCTT TATTTTCCTG 
CCTGCCTGCT TTGGAAACAG CTTTGCGCCA TATCTTAGCA AGATTATTCC TCCCATTCTC 
GCAGGATTGG CAGATGACGT CGACTCCATT CGGGAGACAT CTCTCAAGGC TGGTCGTCTG 
CTGGTCAAGA ACTTCTCTTC CAAGGCTATC GACTTGCTTC TTCCTGAGCT TGAACGCGGT 
CTTGCGGACG ATAGCTACCG TATCAGATTG AGCTCTGTTG AACTGGTGGG TGACCTGCTC 
TTTAGCATCA CGGGTATCAC AGCCAAGGCA GATGCGGAAG AGGAAGAGGA AGAGGCTGCT 
CAAGCCGGAC AGTCCTTGCT CGAGGTCCTC GGCGAGGAGA GGAGAAACAA GGTCTTGTCC 
GCTCTGTTCA TTTGTCGCTG CGATACTTCT GGTCTTGTCA AGAGCGCAGC GATGGCTGTT 
TGGAAGTCGC TTGTCGCTTC ACCCAAAACG CTCAAAGAGA TGGTGCCTAC TTTGTCGCAG 
TTTATCATTC GGCGTCTTGG TTCAGCAAAC ATGGAGCACA AGGTTATCGC CAGCAATGCT 
CTTGGAGACC TTATCAAGAA GGCTGGCGAG TCCGTGCTCG CTACTTTGCT GCCATCTCTC 
GAGGAAGGCC TCCGTACGTC ACCCGATGTG GATGTCAAGC AAGGTATCTG CATTGCCCTG 
AGAGAGCTCA TTACCTCTGC GTCTCCTGAA GCCCTGGAGG ACTACGAAAA GATCCTCATC 
TCGACCGTTC GGGTTGCCCT TGTCGATAGC GACGAAGACG TCCGTGAAGC GGCAGCAGAA 
GCCTTCGATG CACTGCAGCA GATTCTTGGC AAGAAGGCTG TTGACCAGGT TCTGCCCCAT 
CTCCTGATGC TTTTGAGAAA CAACGAGGAT GCTGAGCAAG CGCTGTCAGC ACTACTGACG 
CTCCTTACCG AGCAAACAAG GGCTAACATC ATTTTACCCA ACCTGATCCC GACCCTGATC 
ACTTCGCCCA TCTCTGCCTT TAATGCTAGG GCAATCGCTT CCTTGGCTGA GGTGGCAGGT 
TCGGCCTTGA CGCGCAGATT ACCCGCGATT CTCAACTCCT TGATGGACAA CATTCTTTCC 
ACTACGGACG AGGAGCATCG GGAAGAACTG AACAGCGCCT TCGATGCCGT CTTGGTATCT 
GTGGATGAGT TCGATGGTCT GAATGTCGTG ATGAACGTCA TGATGACTCT TCTGAAACAC 
GATGACCACA GACGCCGCGC CAGCGCTGCG CTCCATCTTA ACAAGTTCTT CTCTGAAGCC 
GAGATCGACT ACTCTAGATA CTACCAGGAT CTGATTCGCG TCCTGCTCAT TTCCTTCGAC 
GATAGCGATA AGGAAGTTGT CAAGGCAGCT TGGACAGCAC TCAGTGGGCT CACGTCTCAC 
ATGCGCAAAG AGGAAATGGA AGTCCTCACT ATTCCAACAC GCCAGGTCCT ACGACAAGTG 
GGTGTGCCTG GAGCCGATCT GCCAGGTTTC AGCTTGCCGA AGGGTATCAC TGCCATTCTG 
CCAATCTTCT TGCAGGGTCT TCTCAACGGC AATGTGGAAC AGCGTACGCA AGCAGCTCTG 
GCCATTGGCG ATATTATCGA CCGTACCAAT GCAAACTCCC TGAAGCTTTT CGTTACTCAA 
ATCACTGGTC CTTTGATCAG AGTGGTTTCT GAACGTTCAG TGGATATCAA ATGTGCTATC 
TTCTTCACAC TTAACAAGCT TCTTGAGAAG ATCCCGATGG CAGTCAAGCC ATTCCTGCCT 
CAGCTGCAAC GTACGTTTGC GCGAGGTCTA GCCGATTCTA CCAGTGAGAC ACTGCGCAAC 
AGGGCTGCCA AGGGTCTTGG AATCTTGATT ACCTTGACTC CAAGGGTGGA TCCCCTCATT 
GCAGAATTGA TTACGGGTAC AAAGACAGAT GATATGGGCG TCAAGAATGC GATGATGAAG 
GCACTACAAG AAGTCGTCGG CAAAGCTGGC GCGAATATGA GCGAGGCTTC GAAGAATGCT 
ATCCTTGCAC TCATTGACGA TGATGCCAGC GATCAGACCG ATGGTGTTGC TATTACGAAT 
GCCAAATTGC TTGGTGCGCT GGTGAAGGTC CTTCCCGCGT CTACCGCCAG CCCACTGATC 
AAGAACCGTG TGCTCACAAG CCATATGACA CACGCATCCA TTCTAGGCCT GAACGCCTTG 
CTGGTTGACT CACCGTCGTC CCTGACTGAG ACTTACGCTG CCGAGACTCA ATCGATCATA 
TGTCAGGGCG TCTCGAACAA GGATCCTTTC ATTGCCGAGA ACAGCGTCCT CGCTGCTGGG 
AAGTTCCTTT TGATCGAGGA TGAGAGTCGC AATTTCGAGA CAAACAAGGC CATCTTTGAA 
GCACTAGCGC CCTGCATACA GGCGGGCGTG CCTCCTGATG TTCGCCGTCT CACTTTGGTG 
GTCTTGAGAA CGGTGAGCCG ACTTCACCCC GAACTGACCC GGCCTCATCT GGCTCTGCTT 
GCACCACCGA TTTTCAGCTG TGTTCGTGAT GTCATTATTC CCATCAAGCT CGCTGCTGAA 
GCTGCGTTCC TGTCACTCTT CTCGGTTGAG GAATCTGAAA GTGCTGTTTT CGACAAGTAT 
ATGGCTGGAC CCGGGGCTGA ACTTCCCCCT GGACCTAAGC GGAGTATGTC GGACTACTTC 
AAGCGAGTTG CGCTACGTCT CGCCGGCCAG GCTAGAGAAC GCAAGGAGGC TGAAGGTGGA 
CAGGGAGGAC TGGGTCTGTC CAATGACGAA GCTGAGGATG AGAAGGAACT GTGGAGCGTC 
GGAAAGGTCG ATCTTGGGGA AGGCTCATTT GCTGATGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001260310.1
CDS	1..8022
Translation	MDDFPWEQVKSGDLESLRDALFSSSTTRRTHALQELRDRVGSELPQEIRQSLIGLLFTTYPLYVDRPSRQAVQQCLRTILKAPVPTEDLKYLTQNLLAEASKPGLAPSSAFVLLEWCSLLLQILKNDPDTPLSIVLDIVAVDAKALETCLAARPRPTVKQSALTVTRRALRAVFSSDNWGEDAVRQSVARLTGDSAGQKNAPFLGVVSGVCAHLPNRKAVLENEKKLILAYYVKELIGSKAAVPNHIASGLSDFFASFITYEDFTSELVPPLEKSLLRAPEVVLGGLVPSLCSSLPEDFDLSEILFSRLLKHLLSSMKSNNATIRQGAVQSLEYILSKSKNEDWLVKIAGEIIGPLKTQKITSPEHRAVYAQAVCAILPSGSVSKDIVQGFAPVFSRESNEVALEQEIKALCKHLTFLLQTKVKVSDDVINAVVKGIADKRIPFRKLWQLIVGDVFWRIEAPTLATSEVGPFVTKFLDKMKDLFKEVTSNPLPSAQNGSLSAAFVYLALYERVSTAQGLDKADWETTVAQSMLLTPKPSFLLNPKAYTKLTSPAEIQWMVRALVAVSSGSKFESTEDAAKVAWAQAFIYAVTAPGLRTNLREEAAHALMGIYLRRSELMGRVVTEALWTWILALRTAEKDSAASSAGPASEKLLHLVAKAICPSAADIQSDKIRSDLKRQLVQLLVLCRPELIPNISWIALCLRTGTDPGDLVREYPDDFMKQLTQVHEDPIQSKVPAIDVAIWNAAGDLAFVAPDTMVPRLIRQIRDDLDASRLSRFTPTDAAIARTPEGTMFVDVLNTKSMQPAFDKNTKDYDTLKWEEELRAQLAQKKGQTQKKLSADEQAKVKAQLAKESKIREEVLQEVKRVERGAGIIQGLASGPAVDADGWINPAVSSLLSLARAGVGLFAGDVVSKAYVKCAEKVSSRLGPLRPFVGVATLRAIGRSHLPPEMEAEPLGGLVTRILYRLRFASEQRPFDMTSLAYILPLIFMVLSRNGIEEVKGEEEGEQLLLALEFLSFHSGSFTDNRLPRVEVLNHLLTAMQKFTQHYKLIKDTLFDFCRCISSNITNEELNVLLQGTIIPDTSVRTSVLQVIESEIDLTDLDFSEHIWLGCHDQVEENAEIAENIWEENALEVDETSYGKIIPYLSSKDSQLRGAAARALGHAVELNPSVFGDIVQQLQSMYEEEVKPKEPAKDKYGMPLKVDTTDHWECRSGIALAFGAMTNSFEGDQIVSFLRFLIERGPLIDRNSMVRSQMADSGRSVVASRGQQRVEELMEILETTLETSDKGSETSDLLNEAVVVLYGSLAQHLKSDDPRLQTVIKRLLATLPTPSETVQSAVSGCLPPLIRLCGPKSGEYVQEMLDQLLQTKNYATQRGAAYGLAGIVRGRGIFTLREYQVMSHLKDATENKKEAHQRLGALLAFELFATILGRTFEPYVIQIVPQLLAGFGDPSIDVRDACLDAAKACFSNLSSYGVKKILPTLLDGLDDTQWRSQKGACDLLGAMAYLDPQQLAASLPDIIPPLTVVLNDTHKEVRNAANRSLQRFGEVISNPEVKGLVNVLLKALSDPTKYTDEALDALIKVSFVHYLDAPSLALVVRILERGLGDRSNTKRKSAQIIGSLAHLTERKDLISHLPIIVSGLHLAIVDPVPTTRATASKALGSLIEKLGEDALPDLIPNLMTTLKSDTGAGDRLGSAQALAEVLAGLGTTRLEETLPTILQNVSSSKPAVREGFMTLFIFLPACFGNSFAPYLSKIIPPILAGLADDVDSIRETSLKAGRLLVKNFSSKAIDLLLPELERGLADDSYRIRLSSVELVGDLLFSITGITAKADAEEEEEEAAQAGQSLLEVLGEERRNKVLSALFICRCDTSGLVKSAAMAVWKSLVASPKTLKEMVPTLSQFIIRRLGSANMEHKVIASNALGDLIKKAGESVLATLLPSLEEGLRTSPDVDVKQGICIALRELITSASPEALEDYEKILISTVRVALVDSDEDVREAAAEAFDALQQILGKKAVDQVLPHLLMLLRNNEDAEQALSALLTLLTEQTRANIILPNLIPTLITSPISAFNARAIASLAEVAGSALTRRLPAILNSLMDNILSTTDEEHREELNSAFDAVLVSVDEFDGLNVVMNVMMTLLKHDDHRRRASAALHLNKFFSEAEIDYSRYYQDLIRVLLISFDDSDKEVVKAAWTALSGLTSHMRKEEMEVLTIPTRQVLRQVGVPGADLPGFSLPKGITAILPIFLQGLLNGNVEQRTQAALAIGDIIDRTNANSLKLFVTQITGPLIRVVSERSVDIKCAIFFTLNKLLEKIPMAVKPFLPQLQRTFARGLADSTSETLRNRAAKGLGILITLTPRVDPLIAELITGTKTDDMGVKNAMMKALQEVVGKAGANMSEASKNAILALIDDDASDQTDGVAITNAKLLGALVKVLPASTASPLIKNRVLTSHMTHASILGLNALLVDSPSSLTETYAAETQSIICQGVSNKDPFIAENSVLAAGKFLLIEDESRNFETNKAIFEALAPCIQAGVPPDVRRLTLVVLRTVSRLHPELTRPHLALLAPPIFSCVRDVIIPIKLAAEAAFLSLFSVEESESAVFDKYMAGPGAELPPGPKRSMSDYFKRVALRLAGQARERKEAEGGQGGLGLSNDEAEDEKELWSVGKVDLGEGSFADE

Target ORF information:

RefSeq Version	XM_001260309.1
Organism	Aspergillus fischeri NRRL 181(Neosartorya fischeri NRRL 181)
Definition	Aspergillus fischeri NRRL 181 translational activator GCN1 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001260309.1

ORF Insert Sequence:

ATGGACGACT TTCCATGGGA ACAGGTGAAA TCAGGAGACC TGGAGAGCCT GCGAGACGCT 
CTCTTTTCGA GCTCGACTAC TCGCAGAACT CATGCTCTCC AGGAGCTCCG CGATAGAGTT 
GGCTCTGAAT TACCCCAAGA AATCCGTCAG TCTCTTATAG GACTCCTTTT TACGACCTAT 
CCGCTCTACG TCGATCGACC CTCTCGACAA GCTGTCCAGC AATGTCTGCG TACGATCCTC 
AAGGCGCCCG TTCCGACCGA AGATCTGAAA TACCTTACCC AGAATCTGCT CGCAGAGGCT 
TCTAAACCCG GATTGGCTCC TTCTTCCGCG TTTGTCCTGC TGGAATGGTG CTCCCTTCTG 
CTGCAGATAT TGAAAAATGA CCCGGACACC CCTCTCTCGA TCGTCCTGGA CATTGTCGCT 
GTGGATGCAA AGGCTCTCGA AACCTGCCTT GCTGCTCGTC CCAGACCAAC GGTGAAACAG 
TCTGCCCTTA CTGTTACGAG ACGTGCTCTA CGCGCCGTTT TCTCGTCAGA CAATTGGGGC 
GAGGATGCTG TCCGCCAGTC AGTCGCTCGG CTGACTGGTG ACTCGGCGGG GCAGAAGAAT 
GCGCCCTTCC TCGGTGTCGT CTCTGGTGTA TGCGCCCACT TACCGAACAG GAAGGCCGTC 
CTGGAGAATG AAAAGAAATT GATCCTCGCA TACTATGTCA AAGAATTGAT CGGCTCAAAA 
GCTGCAGTGC CAAACCACAT CGCAAGTGGC TTATCTGACT TCTTCGCCTC TTTTATTACC 
TATGAGGATT TCACGTCGGA GCTAGTACCT CCTCTTGAGA AATCGCTCTT GCGTGCTCCA 
GAGGTGGTAT TGGGTGGATT GGTGCCTTCA CTATGCTCTT CTCTACCAGA GGACTTTGAC 
CTGTCCGAGA TTCTGTTCTC TCGGCTCCTG AAGCACCTTC TCTCCAGCAT GAAATCAAAC 
AATGCGACTA TTAGGCAAGG TGCTGTGCAG TCTCTTGAGT ACATCCTCTC AAAGTCTAAA 
AATGAGGATT GGCTTGTCAA GATCGCTGGT GAGATCATCG GGCCCCTAAA GACCCAGAAG 
ATCACTAGCC CAGAACATCG TGCTGTGTAT GCTCAAGCGG TCTGTGCTAT CCTGCCTAGT 
GGGAGTGTCT CCAAAGACAT AGTGCAAGGG TTTGCTCCTG TCTTCTCGCG GGAATCTAAC 
GAAGTAGCAT TGGAACAAGA AATCAAGGCC CTCTGCAAGC ACCTCACTTT CCTCCTTCAA 
ACCAAGGTGA AAGTTAGTGA CGATGTCATC AATGCCGTCG TCAAAGGAAT TGCAGATAAG 
CGTATCCCGT TCAGGAAGCT TTGGCAACTG ATTGTTGGAG ACGTCTTCTG GCGTATTGAA 
GCACCAACAC TGGCTACCTC CGAGGTTGGA CCATTTGTCA CCAAGTTCCT CGACAAGATG 
AAGGATTTGT TCAAGGAGGT AACTTCAAAT CCTCTACCTT CAGCTCAGAA TGGCTCTTTA 
TCCGCAGCAT TTGTATACCT TGCCCTCTAC GAAAGGGTAT CTACAGCCCA GGGATTAGAT 
AAGGCGGACT GGGAAACCAC TGTGGCACAG TCGATGCTCC TCACCCCAAA GCCTTCTTTT 
CTTCTGAACC CTAAGGCTTA TACCAAATTG ACGTCTCCAG CAGAGATCCA GTGGATGGTT 
CGCGCCCTTG TGGCTGTCTC GTCTGGATCC AAGTTCGAGA GCACCGAAGA TGCAGCCAAG 
GTTGCATGGG CTCAGGCATT CATTTACGCT GTCACGGCTC CCGGACTTCG CACAAACTTG 
CGTGAGGAAG CTGCTCACGC TCTTATGGGG ATTTATCTGC GCAGGTCGGA ACTGATGGGT 
CGCGTGGTCA CTGAAGCTCT GTGGACGTGG ATTCTCGCCT TGAGAACGGC GGAGAAAGAT 
TCTGCTGCAT CGTCTGCAGG ACCGGCGAGT GAGAAGCTCC TTCACCTCGT AGCGAAGGCC 
ATCTGTCCTT CTGCCGCCGA TATACAGTCC GATAAGATTC GATCTGATCT GAAGCGTCAA 
CTCGTTCAGC TTCTTGTACT TTGCAGGCCA GAACTGATTC CCAATATTTC ATGGATCGCA 
CTGTGCTTAA GGACTGGTAC AGATCCTGGT GATCTTGTCC GTGAATATCC GGATGATTTT 
ATGAAGCAAT TGACGCAGGT GCATGAGGAC CCCATCCAAT CGAAAGTCCC TGCGATTGAT 
GTAGCTATCT GGAATGCTGC AGGGGACCTG GCATTTGTCG CACCAGACAC TATGGTCCCT 
CGCCTGATTC GGCAGATCAG AGACGATTTG GATGCATCGC GTCTTTCTAG GTTCACACCA 
ACCGATGCAG CCATTGCCCG CACTCCAGAG GGGACCATGT TCGTTGATGT CCTCAACACC 
AAGTCGATGC AGCCAGCATT CGACAAGAAC ACCAAAGACT ACGACACCCT CAAATGGGAA 
GAAGAGCTGC GGGCGCAATT AGCTCAGAAG AAAGGTCAAA CGCAGAAGAA GCTTAGCGCG 
GATGAACAAG CGAAGGTCAA AGCTCAACTT GCCAAGGAAT CTAAGATCCG TGAGGAGGTC 
CTACAGGAAG TGAAGAGAGT CGAAAGAGGC GCCGGCATTA TTCAGGGGCT TGCGAGCGGG 
CCCGCCGTTG ATGCTGATGG ATGGATCAAC CCGGCTGTAA GCTCATTGCT GTCTCTGGCC 
CGAGCTGGCG TGGGCTTGTT CGCGGGTGAC GTCGTTTCCA AAGCGTACGT GAAATGCGCT 
GAGAAAGTCT CCTCGCGCCT AGGTCCTCTT CGCCCCTTCG TTGGTGTTGC GACACTACGA 
GCAATTGGCA GGTCCCATCT GCCGCCTGAG ATGGAAGCCG AGCCACTCGG TGGGCTTGTT 
ACAAGAATTC TTTATCGCCT TCGTTTTGCG TCTGAGCAGC GCCCATTCGA CATGACATCC 
TTGGCATATA TACTTCCCTT GATATTCATG GTTCTGAGCC GCAATGGCAT CGAAGAAGTC 
AAAGGGGAAG AAGAAGGAGA GCAGTTGCTT CTGGCGCTGG AGTTCCTGTC ATTCCATTCC 
GGTTCATTCA CTGACAACCG TCTTCCACGC GTTGAGGTTC TGAATCATCT GCTTACTGCC 
ATGCAGAAGT TTACCCAGCA CTACAAGCTG ATCAAGGATA CTCTCTTCGA TTTCTGTAGA 
TGCATTTCGT CCAACATCAC CAATGAGGAG CTTAATGTCC TTCTACAAGG CACCATCATT 
CCAGATACAT CCGTCCGAAC CTCGGTGCTC CAGGTTATTG AGTCTGAAAT TGATCTGACT 
GACCTGGACT TCTCTGAGCA CATCTGGTTG GGCTGTCACG ATCAGGTGGA GGAGAACGCG 
GAAATTGCTG AAAATATCTG GGAGGAAAAT GCCCTCGAGG TCGACGAGAC TTCCTACGGG 
AAAATAATCC CATACCTCTC TTCCAAGGAC TCCCAACTCC GAGGCGCTGC TGCCCGCGCG 
TTGGGCCATG CAGTGGAGCT CAATCCGTCG GTTTTCGGGG ACATTGTCCA GCAATTGCAG 
TCAATGTACG AGGAGGAAGT GAAGCCCAAA GAGCCTGCGA AGGACAAGTA CGGAATGCCG 
CTCAAGGTGG ATACGACCGA CCACTGGGAA TGCCGCAGTG GCATTGCCCT CGCTTTTGGT 
GCGATGACAA ATAGTTTCGA AGGCGATCAG ATTGTTTCGT TCCTTCGCTT TCTGATTGAA 
AGAGGACCCT TGATTGACAG AAACTCTATG GTGAGATCCC AAATGGCTGA CAGCGGCAGA 
TCCGTCGTCG CGTCGCGCGG CCAGCAGCGG GTTGAGGAGT TGATGGAAAT ACTGGAAACC 
ACTCTGGAAA CTTCAGACAA AGGAAGCGAG ACATCCGATC TTCTCAATGA AGCAGTGGTT 
GTTCTCTACG GTTCTCTGGC TCAGCATCTC AAGTCGGACG ATCCGCGGTT GCAAACAGTA 
ATCAAGCGAC TTCTGGCGAC ACTCCCGACT CCGTCGGAAA CTGTACAATC GGCCGTCTCG 
GGATGCCTTC CACCACTCAT TCGTCTCTGC GGGCCAAAGA GCGGAGAGTA TGTACAAGAG 
ATGCTGGATC AACTGTTGCA GACCAAGAAC TATGCTACCC AGCGTGGAGC GGCTTACGGT 
CTGGCTGGTA TCGTTCGTGG AAGGGGCATC TTCACTCTGC GGGAGTACCA GGTCATGTCT 
CACCTGAAGG ACGCAACTGA GAATAAGAAA GAGGCACATC AGCGACTGGG TGCTCTGTTA 
GCGTTTGAAC TCTTCGCCAC CATCCTCGGG CGCACATTTG AACCATATGT CATTCAGATC 
GTGCCGCAGC TCCTTGCCGG TTTCGGTGAC CCTAGCATCG ACGTCCGCGA TGCTTGCCTC 
GACGCAGCAA AGGCTTGCTT CTCTAATCTC AGCTCCTACG GTGTGAAGAA GATTCTCCCT 
ACTCTCCTCG ATGGTCTTGA CGACACGCAA TGGCGCAGTC AAAAGGGAGC CTGCGATCTC 
CTTGGAGCAA TGGCTTATCT CGACCCCCAG CAATTGGCAG CAAGCTTGCC CGACATCATC 
CCTCCACTTA CCGTGGTGCT CAATGATACT CACAAGGAGG TGCGCAACGC CGCAAATCGC 
AGTCTGCAGC GATTCGGTGA GGTCATCAGC AACCCCGAAG TGAAGGGCTT GGTCAATGTC 
CTTCTCAAGG CCCTGAGTGA TCCGACCAAA TACACAGATG AGGCTCTGGA TGCACTTATC 
AAGGTCTCTT TCGTTCATTA TTTGGATGCT CCCTCTTTGG CGCTTGTTGT CCGTATCCTT 
GAGCGCGGTC TTGGCGACAG ATCAAATACC AAGAGGAAGT CCGCACAGAT CATTGGCAGC 
TTGGCTCACC TTACTGAGCG GAAGGATCTT ATCTCTCACC TGCCCATTAT CGTTTCTGGA 
TTGCACTTAG CCATCGTCGA TCCAGTGCCT ACTACCCGTG CCACCGCGTC CAAAGCTCTC 
GGCTCACTTA TCGAGAAGCT CGGAGAGGAT GCACTACCCG ATCTCATTCC CAACCTCATG 
ACTACGCTCA AGTCGGATAC TGGCGCCGGC GATCGTTTGG GATCCGCACA GGCTCTTGCG 
GAGGTTCTGG CAGGTCTGGG AACGACAAGA CTGGAAGAGA CACTGCCAAC AATCTTGCAG 
AACGTCTCAA GCTCCAAGCC GGCTGTTCGG GAAGGCTTCA TGACGCTCTT TATTTTCCTG 
CCTGCCTGCT TTGGAAACAG CTTTGCGCCA TATCTTAGCA AGATTATTCC TCCCATTCTC 
GCAGGATTGG CAGATGACGT CGACTCCATT CGGGAGACAT CTCTCAAGGC TGGTCGTCTG 
CTGGTCAAGA ACTTCTCTTC CAAGGCTATC GACTTGCTTC TTCCTGAGCT TGAACGCGGT 
CTTGCGGACG ATAGCTACCG TATCAGATTG AGCTCTGTTG AACTGGTGGG TGACCTGCTC 
TTTAGCATCA CGGGTATCAC AGCCAAGGCA GATGCGGAAG AGGAAGAGGA AGAGGCTGCT 
CAAGCCGGAC AGTCCTTGCT CGAGGTCCTC GGCGAGGAGA GGAGAAACAA GGTCTTGTCC 
GCTCTGTTCA TTTGTCGCTG CGATACTTCT GGTCTTGTCA AGAGCGCAGC GATGGCTGTT 
TGGAAGTCGC TTGTCGCTTC ACCCAAAACG CTCAAAGAGA TGGTGCCTAC TTTGTCGCAG 
TTTATCATTC GGCGTCTTGG TTCAGCAAAC ATGGAGCACA AGGTTATCGC CAGCAATGCT 
CTTGGAGACC TTATCAAGAA GGCTGGCGAG TCCGTGCTCG CTACTTTGCT GCCATCTCTC 
GAGGAAGGCC TCCGTACGTC ACCCGATGTG GATGTCAAGC AAGGTATCTG CATTGCCCTG 
AGAGAGCTCA TTACCTCTGC GTCTCCTGAA GCCCTGGAGG ACTACGAAAA GATCCTCATC 
TCGACCGTTC GGGTTGCCCT TGTCGATAGC GACGAAGACG TCCGTGAAGC GGCAGCAGAA 
GCCTTCGATG CACTGCAGCA GATTCTTGGC AAGAAGGCTG TTGACCAGGT TCTGCCCCAT 
CTCCTGATGC TTTTGAGAAA CAACGAGGAT GCTGAGCAAG CGCTGTCAGC ACTACTGACG 
CTCCTTACCG AGCAAACAAG GGCTAACATC ATTTTACCCA ACCTGATCCC GACCCTGATC 
ACTTCGCCCA TCTCTGCCTT TAATGCTAGG GCAATCGCTT CCTTGGCTGA GGTGGCAGGT 
TCGGCCTTGA CGCGCAGATT ACCCGCGATT CTCAACTCCT TGATGGACAA CATTCTTTCC 
ACTACGGACG AGGAGCATCG GGAAGAACTG AACAGCGCCT TCGATGCCGT CTTGGTATCT 
GTGGATGAGT TCGATGGTCT GAATGTCGTG ATGAACGTCA TGATGACTCT TCTGAAACAC 
GATGACCACA GACGCCGCGC CAGCGCTGCG CTCCATCTTA ACAAGTTCTT CTCTGAAGCC 
GAGATCGACT ACTCTAGATA CTACCAGGAT CTGATTCGCG TCCTGCTCAT TTCCTTCGAC 
GATAGCGATA AGGAAGTTGT CAAGGCAGCT TGGACAGCAC TCAGTGGGCT CACGTCTCAC 
ATGCGCAAAG AGGAAATGGA AGTCCTCACT ATTCCAACAC GCCAGGTCCT ACGACAAGTG 
GGTGTGCCTG GAGCCGATCT GCCAGGTTTC AGCTTGCCGA AGGGTATCAC TGCCATTCTG 
CCAATCTTCT TGCAGGGTCT TCTCAACGGC AATGTGGAAC AGCGTACGCA AGCAGCTCTG 
GCCATTGGCG ATATTATCGA CCGTACCAAT GCAAACTCCC TGAAGCTTTT CGTTACTCAA 
ATCACTGGTC CTTTGATCAG AGTGGTTTCT GAACGTTCAG TGGATATCAA ATGTGCTATC 
TTCTTCACAC TTAACAAGCT TCTTGAGAAG ATCCCGATGG CAGTCAAGCC ATTCCTGCCT 
CAGCTGCAAC GTACGTTTGC GCGAGGTCTA GCCGATTCTA CCAGTGAGAC ACTGCGCAAC 
AGGGCTGCCA AGGGTCTTGG AATCTTGATT ACCTTGACTC CAAGGGTGGA TCCCCTCATT 
GCAGAATTGA TTACGGGTAC AAAGACAGAT GATATGGGCG TCAAGAATGC GATGATGAAG 
GCACTACAAG AAGTCGTCGG CAAAGCTGGC GCGAATATGA GCGAGGCTTC GAAGAATGCT 
ATCCTTGCAC TCATTGACGA TGATGCCAGC GATCAGACCG ATGGTGTTGC TATTACGAAT 
GCCAAATTGC TTGGTGCGCT GGTGAAGGTC CTTCCCGCGT CTACCGCCAG CCCACTGATC 
AAGAACCGTG TGCTCACAAG CCATATGACA CACGCATCCA TTCTAGGCCT GAACGCCTTG 
CTGGTTGACT CACCGTCGTC CCTGACTGAG ACTTACGCTG CCGAGACTCA ATCGATCATA 
TGTCAGGGCG TCTCGAACAA GGATCCTTTC ATTGCCGAGA ACAGCGTCCT CGCTGCTGGG 
AAGTTCCTTT TGATCGAGGA TGAGAGTCGC AATTTCGAGA CAAACAAGGC CATCTTTGAA 
GCACTAGCGC CCTGCATACA GGCGGGCGTG CCTCCTGATG TTCGCCGTCT CACTTTGGTG 
GTCTTGAGAA CGGTGAGCCG ACTTCACCCC GAACTGACCC GGCCTCATCT GGCTCTGCTT 
GCACCACCGA TTTTCAGCTG TGTTCGTGAT GTCATTATTC CCATCAAGCT CGCTGCTGAA 
GCTGCGTTCC TGTCACTCTT CTCGGTTGAG GAATCTGAAA GTGCTGTTTT CGACAAGTAT 
ATGGCTGGAC CCGGGGCTGA ACTTCCCCCT GGACCTAAGC GGAGTATGTC GGACTACTTC 
AAGCGAGTTG CGCTACGTCT CGCCGGCCAG GCTAGAGAAC GCAAGGAGGC TGAAGGTGGA 
CAGGGAGGAC TGGGTCTGTC CAATGACGAA GCTGAGGATG AGAAGGAACT GTGGAGCGTC 
GGAAAGGTCG ATCTTGGGGA AGGCTCATTT GCTGATGAGT AG