ACA8 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACA8
Entrez Gene ID	835815
Full Name	autoinhibited Ca2+ -ATPase
Synonyms	''AUTOINHIBITED CA2+ -ATPASE,''autoinhibited Ca2+ -ATPase,'autoinhibited Ca2+ -ATPase,AT-ACA8,AUTOINHIBITED CA2+ -ATPASE,ISOFORM 8,ISOFORM 8'',MUL3.5,MUL3_5,autoinhibited Ca2+ -ATPase,isoform 8,isoform 8',isoform 8''
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001345242.1	NP_001331281.1	autoinhibited Ca2+ -ATPase, isoform 8
NM_180869.3	NP_851200.1	autoinhibited Ca2+ -ATPase, isoform 8
NM_125093.6	NP_200521.3	autoinhibited Ca2+ -ATPase, isoform 8

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP2B4	XP_005640818.1
Arabidopsis thaliana (thale cress)	ACA10	NP_194719.1
	ATP2B4	NP_001028098.1
Mus musculus (house mouse)	Atp2b4	NP_998781.1
Rattus norvegicus (Norway rat)	Atp2b4	NP_001005871.1
Magnaporthe oryzae (rice blast fungus)	MGG_04890	XP_003712375.1
Schizosaccharomyces pombe (fission yeast)	SPAPB2B4.04c	NP_593890.1
Arabidopsis thaliana (thale cress)	ACA8	NP_200521.3
Oryza sativa (rice)	Os04g0605500	NP_001053795.1
Homo sapiens (human)	ATP2B4	NP_001675.3
Gallus gallus (chicken)	ATP2B4	XP_004948503.1
Arabidopsis thaliana (thale cress)	ACA9	NP_188755.2
Xenopus tropicalis (tropical clawed frog)	atp2b4	XP_004910734.1
Pan troglodytes (chimpanzee)	ATP2B4	XP_001156333.2
Bos taurus (cattle)	ATP2B4	NP_001166065.1
Danio rerio (zebrafish)	atp2b1b	NP_001129103.1
Neurospora crassa	NCU05154	XP_956652.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Tabata S, Kaneko T, Nakamura Y, Kotani H, Kato T, Asamizu E, Miyajima N, Sasamoto S, Kimura T, Hosouchi T, Kawashima K, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Naruo K, Okumura S, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Sato S, de la Bastide M, Huang E, Spiegel L, Gnoj L, O'Shaughnessy A, Preston R, Habermann K, Murray J, Johnson D, Rohlfing T, Nelson J, Stoneking T, Pepin K, Spieth J, Sekhon M, Armstrong J, Becker M, Belter E, Cordum H, Cordes M, Courtney L, Courtney W, Dante M, Du H, Edwards J, Fryman J, Haakensen B, Lamar E, Latreille P, Leonard S, Meyer R, Mulvaney E, Ozersky P, Riley A, Strowmatt C, Wagner-McPherson C, Wollam A, Yoakum M, Bell M, Dedhia N, Parnell L, Shah R, Rodriguez M, See LH, Vil D, Baker J, Kirchoff K, Toth K, King L, Bahret A, Miller B, Marra M, Martienssen R, McCombie WR, Wilson RK, Murphy G, Bancroft I, Volckaert G, Wambutt R, D?sterh?ft A, Stiekema W, Pohl T, Entian KD, Terryn N, Hartley N, Bent E, Johnson S, Langham SA, McCullagh B, Robben J, Grymonprez B, Zimmermann W, Ramsperger U, Wedler H, Balke K, Wedler E, Peters S, van Staveren M, Dirkse W, Mooijman P, Lankhorst RK, Weitzenegger T, Bothe G, Rose M, Hauf J, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Villarroel R, Gielen J, Ardiles W, Bents O, Lemcke K, Kolesov G, Mayer K, Rudd S, Schoof H, Schueller C, Zaccaria P, Mewes HW, Bevan M, Fransz P, , ,
Nature408(6814)823-6(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CML36 interacts directly with the regulative N terminus of the Arabidopsis plasma membrane Ca(2+)-ATPase isoform 8 (ACA8) and this interaction stimulates ACA8 activity

ACA8-mediated Ca(2+) signalling contributes to modulate early seedling development and coordinates root development with nutrient availability.
Title: The plasma membrane-localised Ca(2+)-ATPase ACA8 plays a role in sucrose signalling involved in early seedling development in Arabidopsis.

ACA8 and ACA10 contribute to plant immunity and have a role in plant development.
Title: Plasma membrane calcium ATPases are important components of receptor-mediated signaling in plant immune responses and development.

Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana.
Title: Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana.

regulatory domain works independently of its terminal localization
Title: Plant and animal type 2B Ca2+-ATPases: evidence for a common auto-inhibitory mechanism.

The following ACA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAb21651	NM_180869.3 Latest version!	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb21651	NM_125093.6 Latest version!	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OAb21651	NM_001345242.1 Latest version!	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACA8 ( NM_001345242.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001331281.1 Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

Price & Availability↑

CloneID	OAb21651
Clone ID Related Accession (Same CDS sequence)	NM_001345242.1 , NM_180869.3 , NM_125093.6
Accession Version	NM_001345242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	autoinhibited Ca2+ -ATPase, isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076).

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001331281.1
CDS	231..3455
Translation	MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRFRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL

Target ORF information:

RefSeq Version	NM_001345242.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001345242.1

ORF Insert Sequence:

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACA8 ( NM_180869.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_851200.1 Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

Price & Availability↑

CloneID	OAb21651
Clone ID Related Accession (Same CDS sequence)	NM_001345242.1 , NM_180869.3 , NM_125093.6
Accession Version	NM_180869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	autoinhibited Ca2+ -ATPase, isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Apr 24, 2008 this sequence version replaced NM_180869.2.

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851200.1
CDS	122..3346
Translation	MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRFRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL

Target ORF information:

RefSeq Version	NM_180869.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180869.3

ORF Insert Sequence:

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACA8 ( NM_125093.6 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_200521.3 Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

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CloneID	OAb21651
Clone ID Related Accession (Same CDS sequence)	NM_001345242.1 , NM_180869.3 , NM_125093.6
Accession Version	NM_125093.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	autoinhibited Ca2+ -ATPase, isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003076). On Sep 12, 2016 this sequence version replaced NM_125093.5.

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_200521.3
CDS	280..3504
Translation	MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRFRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL

Target ORF information:

RefSeq Version	NM_125093.6
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana autoinhibited Ca2+ -ATPase, isoform 8 (ACA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_125093.6

ORF Insert Sequence:

ATGACGAGTC TCTTGAAGTC ATCGCCTGGA CGACGCCGTG GAGGCGATGT GGAGTCTGGG 
AAGAGTGAAC ACGCAGACTC TGATAGCGAC ACTTTCTACA TCCCATCGAA GAATGCTTCT 
ATTGAGCGGC TTCAACAGTG GAGAAAAGCT GCACTGGTTC TCAATGCGTC CCGGAGATTC 
CGGTACACCT TGGACTTGAA GAAGGAGCAA GAAACGAGGG AAATGAGACA GAAGATCAGA 
AGTCATGCTC ATGCTCTTTT GGCGGCGAAT CGTTTTATGG ATATGGGACG TGAGTCAGGA 
GTTGAAAAAA CAACTGGGCC TGCAACTCCG GCTGGTGATT TTGGAATTAC TCCTGAACAG 
CTTGTGATAA TGTCAAAGGA TCACAACAGT GGTGCCCTGG AGCAATATGG AGGGACTCAA 
GGATTGGCCA ATTTGCTCAA GACAAATCCA GAGAAAGGTA TCAGTGGCGA TGATGATGAC 
TTGCTAAAGC GCAAGACCAT TTATGGATCA AACACATATC CTCGCAAGAA AGGGAAAGGG 
TTTCTGAGGT TTCTTTGGGA TGCTTGCCAT GATCTCACCT TGATCATTTT AATGGTTGCT 
GCTGTAGCGT CTTTAGCACT TGGGATAAAA ACAGAGGGTA TCAAAGAAGG ATGGTATGAT 
GGAGGAAGCA TTGCATTTGC TGTGATTCTT GTGATTGTTG TGACGGCTGT CAGTGACTAC 
AAACAGTCGC TCCAGTTTCA AAACTTGAAT GATGAAAAGA GAAACATACA TCTGGAGGTG 
TTAAGAGGTG GAAGACGAGT GGAGATTTCG ATCTATGACA TAGTGGTGGG TGATGTCATT 
CCTCTCAACA TTGGCAATCA GGTTCCTGCT GATGGAGTAC TGATATCTGG CCACTCTCTC 
GCCCTTGATG AATCTAGCAT GACTGGAGAG AGCAAAATTG TTAACAAAGA CGCTAACAAG 
GACCCATTCT TAATGTCTGG CTGTAAAGTG GCAGATGGAA ATGGTTCTAT GCTGGTTACT 
GGTGTTGGAG TCAACACTGA ATGGGGATTG CTGATGGCCA GTATTTCTGA AGACAATGGT 
GAAGAAACTC CCTTGCAGGT GCGCTTAAAT GGGGTAGCTA CTTTTATTGG TTCAATTGGC 
TTAGCGGTGG CTGCTGCTGT GCTAGTGATT CTTCTGACTC GATATTTCAC TGGTCACACT 
AAAGACAATA ATGGAGGTCC TCAATTCGTT AAAGGCAAGA CAAAAGTCGG TCATGTTATT 
GATGATGTGG TCAAAGTCCT TACCGTAGCG GTTACAATTG TCGTAGTGGC AGTGCCTGAG 
GGTCTTCCTT TGGCTGTTAC TTTAACCCTT GCCTATTCAA TGAGGAAAAT GATGGCAGAT 
AAGGCTTTGG TGCGGAGGCT ATCTGCTTGC GAGACAATGG GCTCTGCCAC CACTATTTGC 
AGCGATAAAA CTGGAACACT AACTCTGAAT CAGATGACTG TGGTTGAGTC TTATGCTGGG 
GGCAAGAAAA CAGATACTGA ACAATTGCCA GCAACTATCA CTTCGCTAGT AGTTGAAGGA 
ATATCTCAAA ACACAACTGG TAGTATTTTT GTCCCAGAGG GTGGAGGTGA TTTAGAGTAC 
TCTGGTTCAC CAACAGAAAA GGCCATTCTT GGCTGGGGAG TTAAGCTGGG AATGAATTTC 
GAGACAGCCC GATCGCAGTC TTCTATTCTT CATGCTTTTC CATTTAACTC AGAGAAGAAA 
CGTGGTGGTG TTGCTGTAAA AACGGCTGAT GGTGAAGTTC ATGTTCACTG GAAAGGAGCT 
TCTGAAATTG TCCTGGCATC ATGCCGAAGC TACATCGATG AGGATGGTAA TGTGGCACCA 
ATGACTGACG ACAAGGCATC GTTTTTCAAG AATGGCATTA ATGATATGGC TGGAAGAACC 
TTACGATGTG TTGCACTCGC CTTTAGAACC TATGAGGCTG AAAAGGTCCC AACAGGCGAA 
GAGCTCTCAA AGTGGGTACT CCCAGAGGAT GATCTTATAT TATTAGCTAT AGTGGGCATA 
AAGGATCCAT GTAGACCAGG AGTCAAAGAT TCAGTTGTGT TGTGTCAAAA TGCTGGTGTC 
AAGGTCCGTA TGGTTACTGG TGACAACGTC CAAACTGCCA GGGCTATTGC CTTGGAATGT 
GGAATACTAA GTTCAGATGC AGATCTCTCC GAGCCTACTC TCATTGAAGG AAAATCATTC 
CGTGAGATGA CTGATGCAGA AAGGGACAAG ATTAGTGACA AAATATCGGT TATGGGCCGA 
TCATCTCCCA ATGACAAACT TCTGCTGGTA CAATCTCTGA GACGACAAGG GCATGTTGTT 
GCTGTCACCG GGGATGGGAC AAATGATGCT CCTGCATTGC ACGAGGCAGA TATTGGTCTT 
GCTATGGGAA TAGCAGGAAC AGAAGTGGCT AAGGAGAGTT CGGACATCAT CATCTTGGAT 
GACAACTTTG CTTCAGTTGT CAAGGTTGTT CGATGGGGGC GATCAGTGTA TGCCAACATT 
CAGAAGTTCA TCCAATTTCA GCTCACAGTC AATGTCGCCG CTCTCGTAAT TAATGTTGTA 
GCTGCTATTT CGAGTGGTGA CGTTCCACTT ACCGCTGTGC AGCTTCTCTG GGTTAATCTG 
ATAATGGACA CTCTTGGAGC ATTGGCTTTG GCTACTGAAC CACCCACTGA TCACCTCATG 
GGGAGGCCTC CAGTTGGCAG AAAAGAACCT CTTATTACCA ACATCATGTG GAGAAACTTG 
CTGATCCAGG CTATTTATCA AGTGAGTGTA TTGTTAACAC TCAATTTCCG AGGCATAAGC 
ATACTTGGCC TGGAGCATGA AGTGCATGAA CACGCCACTA GAGTGAAGAA CACAATAATC 
TTCAACGCCT TTGTTCTCTG CCAAGCTTTC AATGAGTTCA ATGCTCGTAA ACCAGATGAG 
AAGAACATCT TCAAAGGTGT GATCAAGAAT CGTCTCTTTA TGGGAATAAT AGTCATAACT 
CTTGTGCTCC AGGTTATCAT TGTTGAGTTC CTTGGTAAAT TCGCTTCCAC GACGAAACTA 
AACTGGAAGC AATGGCTTAT CTGTGTTGGC ATCGGTGTTA TCAGTTGGCC TCTTGCTTTG 
GTCGGGAAAT TCATTCCGGT GCCTGCAGCT CCTATAAGCA ACAAATTGAA AGTACTAAAA 
TTCTGGGGCA AGAAGAAAAA TTCTTCTGGA GAAGGTTCAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.
Nature408(6814)823-6(2000 Dec)
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ACA8 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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