UBE4A cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	105415848
Full Name	ubiquitination factor E4A
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011601801.1	XP_011600103.1	ubiquitin conjugation factor E4 A isoform X2
XM_011601800.1	XP_011600102.1	ubiquitin conjugation factor E4 A isoform X1

Pathways from BioSystems
Gene Ontology

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq11409	XM_011601800.1 Latest version!	Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OAq11410	XM_011601801.1 Latest version!	Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( XM_011601800.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011600102.1 Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq11409
Clone ID Related Accession (Same CDS sequence)	XM_011601800.1
Accession Version	XM_011601800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950873.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCATCGCCG ACGCCAAGCA GTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGATG AGACTTCGGT CAGCCAAGAT GACTCCGACA ACAGCGTCAC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCTGAGATC AGCCGCTCCT TCCGCTCGCA GCGGGAGCTG 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATAT TCCTCATCAC CCTCGACAAC 
AGCGACCCCA GCATGAAGAG CGGGAACGGG ATCCCAGCGC GCTGCGTCTA TCTGGAAGAA 
ATGGCCGCGG ACCTGGACGA CCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTC 
TTCACCCGCT TGCTGCTGCC GGAGCCTGGA AACCACCTGA TTTACATGAC CTCCACCAGC 
ACACAGAACC TCTCTGCCGA CCGGGATGCA GGGGAGAGGC AGATTCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAGGTCC CAGAGAACCT GCTGCCCTTC 
GCCGTGCGCT GCCGGAACCT GACCGTGTCA AACACTCGCA CTGTCCTCCT CACCCCGGAG 
ATTTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGATGCTGGA GGCACTGAGA 
GGGGCGCACT TCGAAGACGT GACCGAGTTT CTTGAGGAGG TCATAGAAGC CTTAACAATG 
GATGAGGAGG TGCGGACGTT CGGGGAGGTC ATGGTTCCCG TGTTTGACAT CCTCTTGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTCGA TGTGCTCCTC 
TACTTCACAA AGCAGAAGGA CATAGCAAAG GTTTTTGCAG GCTACATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTAGGTG CCATTTTAAA CATCTCCTGC 
TTGTTAAAGA CCCCCGGCGT AGTGGAGAAC CACGGCTATT TTCTGAATCC ATCCCGATCC 
AGCCCACAAG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGTC CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGTTGT CTCCAGAGAC AAAGCACAGG 
ATTCTCTCCT GGCTGGGAAA CTGCCTGCAC GCGAACGCGG GTCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTCAACTTG 
GGAGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTA 
ACCTTCAACC CCACTTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGCTTGA TACCCGCTCT GAGCGAGCAA 
GAGCCAGAGT TTGCAAATAG CTACAATCTG GTGACGGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTTCA CAGGTTGCAC GACCACATGG TAAAGATAAA CCAAAGCCTT 
CATCGCCTGC AAGTAGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCTGC TGCTGACAGC 
CTCAGGGAGC AGTTCGAGCG CCTGATGACC ATCTATCTCT CCACCAAGAC AGCGATGACA 
GAGCCACAGA TGCTGCAGAA CTGCCTGAAT CTGCAAGTGT CTATGGCGGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TGACCTTCCC GCTGCCAGAG 
GTGGAAAACA GCGCGTTGGC CTATGTGCCA GAATTCTTTG CCGATAATTT GGGTGACTTC 
TTCATTTTCC TGCGGCGTTT CGCTGATGAC ATCTTGGAGA CTTCCGCCGA TTCTCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAT 
CTGCGAGCTA AGCTGGCAGA AGTGTTGGAA GCTGTGATGC CTCACTTAGA TCAGGCCCAG 
AACCCACTCG TCTCGAGTGT GTTTCATCGT AAGCGAGTGT TCTGTTCTTA CCAAAACGCT 
GCCCACCTTG CCGAGGCGCT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGTGACCCG 
CACCAGTTTG AACAGAAGTT TAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACAG ATTCGTACCG GCAGAGCATA AAGGCTCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTCAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGTAAGA TTAAAGTTCA GCAGATCGAG 
AAGGACCGTG GCGAATGGGA CAGCCTGTCT CAGGAGGCTC GCCGCGAGAA GGAGTCAAGC 
CTGCAGATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATTGGC 
ACTCTGGCCT TCCTGACCTC AGAAATTAAG TCCCTGTTTG TGCATCCTTT CCTTGCTGAG 
CGCATCATCT CCATGCTCAA CTATTTCCTG CAACACCTTG TTGGCCCTAA AATGGGAGCT 
CTGAAGGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCCC AGCAGCTCGT GTCTGACATC 
TGTACCATCT ACCTAAACCT TGGGGATGAA GAAAATTTCT GTGCCACGGT GCCCAAGGAC 
GGCCGCTCCT ATTCACCGAT GCTCTTTGCC CAGACTGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCTGGCA ACATGATAGT GTCTTTCAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA AGAGACGTAT GCGGATGCCT GTGATGAATT CCTGGATCCG 
ATCATGAGCA CTCTGATGTC AGACCCGGTG ATACTGCCCT CCTCCCGTGT TACCGTGGAC 
CGGTCGACTA TAGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTCAA TCGGAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTTAAAG AGAAGATCCA GCGGTGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGACAGGC TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600102.1
CDS	37..3264
Translation	MTDQENNNSISSNPFAALFGSIADAKQFAAVQKQQQLRQLTGDETSVSQDDSDNSVTESLDDCDYSVAEISRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPARCVYLEEMAADLDDQDWLDMDNVEQALFTRLLLPEPGNHLIYMTSTSTQNLSADRDAGERQILRYLYACFQRAREEITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVYEQLVDLMLEALRGAHFEDVTEFLEEVIEALTMDEEVRTFGEVMVPVFDILLGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMSQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPALSEQEPEFANSYNLVTENLVLTQYTLHLGFHRLHDHMVKINQSLHRLQVAWREAQQSSSPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAMGNHGTEPLELTFPLPEVENSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHILHFVTVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQNPLVSSVFHRKRVFCSYQNAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRQSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSQEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKKQKEELEDRLD

Target ORF information:

RefSeq Version	XM_011601800.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011601800.1

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCATCGCCG ACGCCAAGCA GTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGATG AGACTTCGGT CAGCCAAGAT GACTCCGACA ACAGCGTCAC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCTGAGATC AGCCGCTCCT TCCGCTCGCA GCGGGAGCTG 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATAT TCCTCATCAC CCTCGACAAC 
AGCGACCCCA GCATGAAGAG CGGGAACGGG ATCCCAGCGC GCTGCGTCTA TCTGGAAGAA 
ATGGCCGCGG ACCTGGACGA CCAGGACTGG CTGGACATGG ACAATGTCGA GCAGGCCCTC 
TTCACCCGCT TGCTGCTGCC GGAGCCTGGA AACCACCTGA TTTACATGAC CTCCACCAGC 
ACACAGAACC TCTCTGCCGA CCGGGATGCA GGGGAGAGGC AGATTCTGCG CTACCTCTAC 
GCCTGCTTCC AGAGGGCAAG AGAAGAGATA ACCAAGGTCC CAGAGAACCT GCTGCCCTTC 
GCCGTGCGCT GCCGGAACCT GACCGTGTCA AACACTCGCA CTGTCCTCCT CACCCCGGAG 
ATTTACGTCA ACCAGAACGT GTACGAGCAG CTGGTGGACC TGATGCTGGA GGCACTGAGA 
GGGGCGCACT TCGAAGACGT GACCGAGTTT CTTGAGGAGG TCATAGAAGC CTTAACAATG 
GATGAGGAGG TGCGGACGTT CGGGGAGGTC ATGGTTCCCG TGTTTGACAT CCTCTTGGGC 
AGAATCAAGG ACCTGGACCT CTGCCAGATC CTGCTGTACA CGTACCTCGA TGTGCTCCTC 
TACTTCACAA AGCAGAAGGA CATAGCAAAG GTTTTTGCAG GCTACATCCA GCCCAAGGAT 
CCCAGCAATG GACAGATGTA CCAGAAGACT TTGCTAGGTG CCATTTTAAA CATCTCCTGC 
TTGTTAAAGA CCCCCGGCGT AGTGGAGAAC CACGGCTATT TTCTGAATCC ATCCCGATCC 
AGCCCACAAG AGATCAAAGT GCAGGAATCC AACATCCACC AGTTTATGTC CCAGTTCCAC 
GAGAAGATCT ACCAGATGCT GAAGAACCTG TTGCAGTTGT CTCCAGAGAC AAAGCACAGG 
ATTCTCTCCT GGCTGGGAAA CTGCCTGCAC GCGAACGCGG GTCGCACCAA GATTTGGGCT 
AACCAGATGC CAGAGATCTT CTTCCAGATG TATGCCTCAG ATGCCTTCTT CCTCAACTTG 
GGAGCTGCCC TCCTGAAGCT GTGCCAGCCC TTCTGCAAAC CAAAATCTCC GAGGCTGCTA 
ACCTTCAACC CCACTTACTG TGCCCTGAAG GAGCTGAATG AAGAGGAGAG GAGGAGCAAG 
AACGTACATA TGAAAGGTTT GGAAAAAGAA ACATGCTTGA TACCCGCTCT GAGCGAGCAA 
GAGCCAGAGT TTGCAAATAG CTACAATCTG GTGACGGAAA ACCTGGTCCT CACACAGTAC 
ACCCTTCACT TGGGATTTCA CAGGTTGCAC GACCACATGG TAAAGATAAA CCAAAGCCTT 
CATCGCCTGC AAGTAGCGTG GCGAGAAGCT CAGCAGAGCT CCAGCCCTGC TGCTGACAGC 
CTCAGGGAGC AGTTCGAGCG CCTGATGACC ATCTATCTCT CCACCAAGAC AGCGATGACA 
GAGCCACAGA TGCTGCAGAA CTGCCTGAAT CTGCAAGTGT CTATGGCGGT TCTGCTGGTG 
CAGCTGGCCA TGGGAAACCA TGGGACGGAG CCGCTAGAGC TGACCTTCCC GCTGCCAGAG 
GTGGAAAACA GCGCGTTGGC CTATGTGCCA GAATTCTTTG CCGATAATTT GGGTGACTTC 
TTCATTTTCC TGCGGCGTTT CGCTGATGAC ATCTTGGAGA CTTCCGCCGA TTCTCTGGAG 
CACATCCTTC ACTTTGTTAC TGTTTTCATG GGTGATGTGG AGAGGATGAA AAATCCTCAT 
CTGCGAGCTA AGCTGGCAGA AGTGTTGGAA GCTGTGATGC CTCACTTAGA TCAGGCCCAG 
AACCCACTCG TCTCGAGTGT GTTTCATCGT AAGCGAGTGT TCTGTTCTTA CCAAAACGCT 
GCCCACCTTG CCGAGGCGCT TATCAAAGTC TTTGTGGATA TCGAGTTTAC TGGTGACCCG 
CACCAGTTTG AACAGAAGTT TAACTACCGC CGTCCCATGT ATCCCATCCT GAGGTACATG 
TGGGGCACAG ATTCGTACCG GCAGAGCATA AAGGCTCTGG CTGATTACGC CTCAGAGAAC 
CTGGAGGCAA TGAACCCCCC TCTCTTCTTG CGCTTCCTTA ATTTGCTCAT GAATGATGCC 
ATTTTCCTGT TGGATGAAGC CATACAGTAC CTCAGTAAGA TTAAAGTTCA GCAGATCGAG 
AAGGACCGTG GCGAATGGGA CAGCCTGTCT CAGGAGGCTC GCCGCGAGAA GGAGTCAAGC 
CTGCAGATGT TTGGTCAGCT GGCACGCTTC CACAACATCA TGTCCAACGA AACCATTGGC 
ACTCTGGCCT TCCTGACCTC AGAAATTAAG TCCCTGTTTG TGCATCCTTT CCTTGCTGAG 
CGCATCATCT CCATGCTCAA CTATTTCCTG CAACACCTTG TTGGCCCTAA AATGGGAGCT 
CTGAAGGTCA AGGATTTCAG TGAGTTTGAC TTCAAGCCCC AGCAGCTCGT GTCTGACATC 
TGTACCATCT ACCTAAACCT TGGGGATGAA GAAAATTTCT GTGCCACGGT GCCCAAGGAC 
GGCCGCTCCT ATTCACCGAT GCTCTTTGCC CAGACTGTTC GGGTTCTGAA GAAAATCAAC 
AAGCCTGGCA ACATGATAGT GTCTTTCAGT AACCTGGCTG AGAGGATCAA GTCTCTTGCA 
GACCGCCAGC AGCAGGAGGA AGAGACGTAT GCGGATGCCT GTGATGAATT CCTGGATCCG 
ATCATGAGCA CTCTGATGTC AGACCCGGTG ATACTGCCCT CCTCCCGTGT TACCGTGGAC 
CGGTCGACTA TAGCCCGGCA CCTCCTCAGT GACCAGACGG ATCCTTTCAA TCGGAGCCCC 
CTCACTATGG ACCAGATCAG ACCAAACACA GAGCTTAAAG AGAAGATCCA GCGGTGGCTT 
GCAGAGAGGA AAAAGCAGAA GGAGGAGCTG GAGGACAGGC TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( XM_011601801.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011600103.1 Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq11410
Clone ID Related Accession (Same CDS sequence)	XM_011601801.1
Accession Version	XM_011601801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950873.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCATCGCCG ACGCCAAGCA GTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGATG AGACTTCGGT CAGCCAAGAT GACTCCGACA ACAGCGTCAC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCTGAGATC AGCCGCTCCT TCCGCTCGCA GCGGGAGCTG 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATAT TCCTCATCAC CCTCGACAAC 
AGCGACCCCA GCATGAAGAG CGGGAACGGG ATCCCAGCGC GCTGCGTCTA TCTGGAAGAA 
ATGGCCGCGG ACCTGGACGA CCAGGACTGG CTGGACATGG ACAATGTCGA GCAGATAACC 
AAGGTCCCAG AGAACCTGCT GCCCTTCGCC GTGCGCTGCC GGAACCTGAC CGTGTCAAAC 
ACTCGCACTG TCCTCCTCAC CCCGGAGATT TACGTCAACC AGAACGTGTA CGAGCAGCTG 
GTGGACCTGA TGCTGGAGGC ACTGAGAGGG GCGCACTTCG AAGACGTGAC CGAGTTTCTT 
GAGGAGGTCA TAGAAGCCTT AACAATGGAT GAGGAGGTGC GGACGTTCGG GGAGGTCATG 
GTTCCCGTGT TTGACATCCT CTTGGGCAGA ATCAAGGACC TGGACCTCTG CCAGATCCTG 
CTGTACACGT ACCTCGATGT GCTCCTCTAC TTCACAAAGC AGAAGGACAT AGCAAAGGTT 
TTTGCAGGCT ACATCCAGCC CAAGGATCCC AGCAATGGAC AGATGTACCA GAAGACTTTG 
CTAGGTGCCA TTTTAAACAT CTCCTGCTTG TTAAAGACCC CCGGCGTAGT GGAGAACCAC 
GGCTATTTTC TGAATCCATC CCGATCCAGC CCACAAGAGA TCAAAGTGCA GGAATCCAAC 
ATCCACCAGT TTATGTCCCA GTTCCACGAG AAGATCTACC AGATGCTGAA GAACCTGTTG 
CAGTTGTCTC CAGAGACAAA GCACAGGATT CTCTCCTGGC TGGGAAACTG CCTGCACGCG 
AACGCGGGTC GCACCAAGAT TTGGGCTAAC CAGATGCCAG AGATCTTCTT CCAGATGTAT 
GCCTCAGATG CCTTCTTCCT CAACTTGGGA GCTGCCCTCC TGAAGCTGTG CCAGCCCTTC 
TGCAAACCAA AATCTCCGAG GCTGCTAACC TTCAACCCCA CTTACTGTGC CCTGAAGGAG 
CTGAATGAAG AGGAGAGGAG GAGCAAGAAC GTACATATGA AAGGTTTGGA AAAAGAAACA 
TGCTTGATAC CCGCTCTGAG CGAGCAAGAG CCAGAGTTTG CAAATAGCTA CAATCTGGTG 
ACGGAAAACC TGGTCCTCAC ACAGTACACC CTTCACTTGG GATTTCACAG GTTGCACGAC 
CACATGGTAA AGATAAACCA AAGCCTTCAT CGCCTGCAAG TAGCGTGGCG AGAAGCTCAG 
CAGAGCTCCA GCCCTGCTGC TGACAGCCTC AGGGAGCAGT TCGAGCGCCT GATGACCATC 
TATCTCTCCA CCAAGACAGC GATGACAGAG CCACAGATGC TGCAGAACTG CCTGAATCTG 
CAAGTGTCTA TGGCGGTTCT GCTGGTGCAG CTGGCCATGG GAAACCATGG GACGGAGCCG 
CTAGAGCTGA CCTTCCCGCT GCCAGAGGTG GAAAACAGCG CGTTGGCCTA TGTGCCAGAA 
TTCTTTGCCG ATAATTTGGG TGACTTCTTC ATTTTCCTGC GGCGTTTCGC TGATGACATC 
TTGGAGACTT CCGCCGATTC TCTGGAGCAC ATCCTTCACT TTGTTACTGT TTTCATGGGT 
GATGTGGAGA GGATGAAAAA TCCTCATCTG CGAGCTAAGC TGGCAGAAGT GTTGGAAGCT 
GTGATGCCTC ACTTAGATCA GGCCCAGAAC CCACTCGTCT CGAGTGTGTT TCATCGTAAG 
CGAGTGTTCT GTTCTTACCA AAACGCTGCC CACCTTGCCG AGGCGCTTAT CAAAGTCTTT 
GTGGATATCG AGTTTACTGG TGACCCGCAC CAGTTTGAAC AGAAGTTTAA CTACCGCCGT 
CCCATGTATC CCATCCTGAG GTACATGTGG GGCACAGATT CGTACCGGCA GAGCATAAAG 
GCTCTGGCTG ATTACGCCTC AGAGAACCTG GAGGCAATGA ACCCCCCTCT CTTCTTGCGC 
TTCCTTAATT TGCTCATGAA TGATGCCATT TTCCTGTTGG ATGAAGCCAT ACAGTACCTC 
AGTAAGATTA AAGTTCAGCA GATCGAGAAG GACCGTGGCG AATGGGACAG CCTGTCTCAG 
GAGGCTCGCC GCGAGAAGGA GTCAAGCCTG CAGATGTTTG GTCAGCTGGC ACGCTTCCAC 
AACATCATGT CCAACGAAAC CATTGGCACT CTGGCCTTCC TGACCTCAGA AATTAAGTCC 
CTGTTTGTGC ATCCTTTCCT TGCTGAGCGC ATCATCTCCA TGCTCAACTA TTTCCTGCAA 
CACCTTGTTG GCCCTAAAAT GGGAGCTCTG AAGGTCAAGG ATTTCAGTGA GTTTGACTTC 
AAGCCCCAGC AGCTCGTGTC TGACATCTGT ACCATCTACC TAAACCTTGG GGATGAAGAA 
AATTTCTGTG CCACGGTGCC CAAGGACGGC CGCTCCTATT CACCGATGCT CTTTGCCCAG 
ACTGTTCGGG TTCTGAAGAA AATCAACAAG CCTGGCAACA TGATAGTGTC TTTCAGTAAC 
CTGGCTGAGA GGATCAAGTC TCTTGCAGAC CGCCAGCAGC AGGAGGAAGA GACGTATGCG 
GATGCCTGTG ATGAATTCCT GGATCCGATC ATGAGCACTC TGATGTCAGA CCCGGTGATA 
CTGCCCTCCT CCCGTGTTAC CGTGGACCGG TCGACTATAG CCCGGCACCT CCTCAGTGAC 
CAGACGGATC CTTTCAATCG GAGCCCCCTC ACTATGGACC AGATCAGACC AAACACAGAG 
CTTAAAGAGA AGATCCAGCG GTGGCTTGCA GAGAGGAAAA AGCAGAAGGA GGAGCTGGAG 
GACAGGCTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011600103.1
CDS	31..3105
Translation	MTDQENNNSISSNPFAALFGSIADAKQFAAVQKQQQLRQLTGDETSVSQDDSDNSVTESLDDCDYSVAEISRSFRSQRELCEQLNINHMIQRIFLITLDNSDPSMKSGNGIPARCVYLEEMAADLDDQDWLDMDNVEQITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVNQNVYEQLVDLMLEALRGAHFEDVTEFLEEVIEALTMDEEVRTFGEVMVPVFDILLGRIKDLDLCQILLYTYLDVLLYFTKQKDIAKVFAGYIQPKDPSNGQMYQKTLLGAILNISCLLKTPGVVENHGYFLNPSRSSPQEIKVQESNIHQFMSQFHEKIYQMLKNLLQLSPETKHRILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNEEERRSKNVHMKGLEKETCLIPALSEQEPEFANSYNLVTENLVLTQYTLHLGFHRLHDHMVKINQSLHRLQVAWREAQQSSSPAADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAMGNHGTEPLELTFPLPEVENSALAYVPEFFADNLGDFFIFLRRFADDILETSADSLEHILHFVTVFMGDVERMKNPHLRAKLAEVLEAVMPHLDQAQNPLVSSVFHRKRVFCSYQNAAHLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDSYRQSIKALADYASENLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKVQQIEKDRGEWDSLSQEARREKESSLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVSFSNLAERIKSLADRQQQEEETYADACDEFLDPIMSTLMSDPVILPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKKQKEELEDRLD

Target ORF information:

RefSeq Version	XM_011601801.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011601801.1

ORF Insert Sequence:

ATGACCGACC AGGAGAACAA CAACAGCATC TCCAGCAACC CCTTCGCCGC CCTCTTCGGC 
TCCATCGCCG ACGCCAAGCA GTTCGCCGCC GTCCAGAAGC AGCAGCAGCT GCGGCAGCTG 
ACAGGCGATG AGACTTCGGT CAGCCAAGAT GACTCCGACA ACAGCGTCAC CGAGAGCCTG 
GACGACTGCG ACTACTCGGT GGCTGAGATC AGCCGCTCCT TCCGCTCGCA GCGGGAGCTG 
TGCGAGCAGC TCAACATCAA CCACATGATC CAGAGGATAT TCCTCATCAC CCTCGACAAC 
AGCGACCCCA GCATGAAGAG CGGGAACGGG ATCCCAGCGC GCTGCGTCTA TCTGGAAGAA 
ATGGCCGCGG ACCTGGACGA CCAGGACTGG CTGGACATGG ACAATGTCGA GCAGATAACC 
AAGGTCCCAG AGAACCTGCT GCCCTTCGCC GTGCGCTGCC GGAACCTGAC CGTGTCAAAC 
ACTCGCACTG TCCTCCTCAC CCCGGAGATT TACGTCAACC AGAACGTGTA CGAGCAGCTG 
GTGGACCTGA TGCTGGAGGC ACTGAGAGGG GCGCACTTCG AAGACGTGAC CGAGTTTCTT 
GAGGAGGTCA TAGAAGCCTT AACAATGGAT GAGGAGGTGC GGACGTTCGG GGAGGTCATG 
GTTCCCGTGT TTGACATCCT CTTGGGCAGA ATCAAGGACC TGGACCTCTG CCAGATCCTG 
CTGTACACGT ACCTCGATGT GCTCCTCTAC TTCACAAAGC AGAAGGACAT AGCAAAGGTT 
TTTGCAGGCT ACATCCAGCC CAAGGATCCC AGCAATGGAC AGATGTACCA GAAGACTTTG 
CTAGGTGCCA TTTTAAACAT CTCCTGCTTG TTAAAGACCC CCGGCGTAGT GGAGAACCAC 
GGCTATTTTC TGAATCCATC CCGATCCAGC CCACAAGAGA TCAAAGTGCA GGAATCCAAC 
ATCCACCAGT TTATGTCCCA GTTCCACGAG AAGATCTACC AGATGCTGAA GAACCTGTTG 
CAGTTGTCTC CAGAGACAAA GCACAGGATT CTCTCCTGGC TGGGAAACTG CCTGCACGCG 
AACGCGGGTC GCACCAAGAT TTGGGCTAAC CAGATGCCAG AGATCTTCTT CCAGATGTAT 
GCCTCAGATG CCTTCTTCCT CAACTTGGGA GCTGCCCTCC TGAAGCTGTG CCAGCCCTTC 
TGCAAACCAA AATCTCCGAG GCTGCTAACC TTCAACCCCA CTTACTGTGC CCTGAAGGAG 
CTGAATGAAG AGGAGAGGAG GAGCAAGAAC GTACATATGA AAGGTTTGGA AAAAGAAACA 
TGCTTGATAC CCGCTCTGAG CGAGCAAGAG CCAGAGTTTG CAAATAGCTA CAATCTGGTG 
ACGGAAAACC TGGTCCTCAC ACAGTACACC CTTCACTTGG GATTTCACAG GTTGCACGAC 
CACATGGTAA AGATAAACCA AAGCCTTCAT CGCCTGCAAG TAGCGTGGCG AGAAGCTCAG 
CAGAGCTCCA GCCCTGCTGC TGACAGCCTC AGGGAGCAGT TCGAGCGCCT GATGACCATC 
TATCTCTCCA CCAAGACAGC GATGACAGAG CCACAGATGC TGCAGAACTG CCTGAATCTG 
CAAGTGTCTA TGGCGGTTCT GCTGGTGCAG CTGGCCATGG GAAACCATGG GACGGAGCCG 
CTAGAGCTGA CCTTCCCGCT GCCAGAGGTG GAAAACAGCG CGTTGGCCTA TGTGCCAGAA 
TTCTTTGCCG ATAATTTGGG TGACTTCTTC ATTTTCCTGC GGCGTTTCGC TGATGACATC 
TTGGAGACTT CCGCCGATTC TCTGGAGCAC ATCCTTCACT TTGTTACTGT TTTCATGGGT 
GATGTGGAGA GGATGAAAAA TCCTCATCTG CGAGCTAAGC TGGCAGAAGT GTTGGAAGCT 
GTGATGCCTC ACTTAGATCA GGCCCAGAAC CCACTCGTCT CGAGTGTGTT TCATCGTAAG 
CGAGTGTTCT GTTCTTACCA AAACGCTGCC CACCTTGCCG AGGCGCTTAT CAAAGTCTTT 
GTGGATATCG AGTTTACTGG TGACCCGCAC CAGTTTGAAC AGAAGTTTAA CTACCGCCGT 
CCCATGTATC CCATCCTGAG GTACATGTGG GGCACAGATT CGTACCGGCA GAGCATAAAG 
GCTCTGGCTG ATTACGCCTC AGAGAACCTG GAGGCAATGA ACCCCCCTCT CTTCTTGCGC 
TTCCTTAATT TGCTCATGAA TGATGCCATT TTCCTGTTGG ATGAAGCCAT ACAGTACCTC 
AGTAAGATTA AAGTTCAGCA GATCGAGAAG GACCGTGGCG AATGGGACAG CCTGTCTCAG 
GAGGCTCGCC GCGAGAAGGA GTCAAGCCTG CAGATGTTTG GTCAGCTGGC ACGCTTCCAC 
AACATCATGT CCAACGAAAC CATTGGCACT CTGGCCTTCC TGACCTCAGA AATTAAGTCC 
CTGTTTGTGC ATCCTTTCCT TGCTGAGCGC ATCATCTCCA TGCTCAACTA TTTCCTGCAA 
CACCTTGTTG GCCCTAAAAT GGGAGCTCTG AAGGTCAAGG ATTTCAGTGA GTTTGACTTC 
AAGCCCCAGC AGCTCGTGTC TGACATCTGT ACCATCTACC TAAACCTTGG GGATGAAGAA 
AATTTCTGTG CCACGGTGCC CAAGGACGGC CGCTCCTATT CACCGATGCT CTTTGCCCAG 
ACTGTTCGGG TTCTGAAGAA AATCAACAAG CCTGGCAACA TGATAGTGTC TTTCAGTAAC 
CTGGCTGAGA GGATCAAGTC TCTTGCAGAC CGCCAGCAGC AGGAGGAAGA GACGTATGCG 
GATGCCTGTG ATGAATTCCT GGATCCGATC ATGAGCACTC TGATGTCAGA CCCGGTGATA 
CTGCCCTCCT CCCGTGTTAC CGTGGACCGG TCGACTATAG CCCGGCACCT CCTCAGTGAC 
CAGACGGATC CTTTCAATCG GAGCCCCCTC ACTATGGACC AGATCAGACC AAACACAGAG 
CTTAAAGAGA AGATCCAGCG GTGGCTTGCA GAGAGGAAAA AGCAGAAGGA GGAGCTGGAG 
GACAGGCTGG ACTGA