NEK1 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	NEK1
Entrez Gene ID	105403076
Full Name	NIMA related kinase 1
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011578841.1	XP_011577143.1	serine/threonine-protein kinase Nek1 isoform X5
XM_011578844.1	XP_011577146.1	serine/threonine-protein kinase Nek1 isoform X8
XM_011578838.1	XP_011577140.1	serine/threonine-protein kinase Nek1 isoform X2
XM_011578843.1	XP_011577145.1	serine/threonine-protein kinase Nek1 isoform X7
XM_011578836.1	XP_011577138.1	serine/threonine-protein kinase Nek1 isoform X1
XM_011578839.1	XP_011577141.1	serine/threonine-protein kinase Nek1 isoform X3
XM_011578840.1	XP_011577142.1	serine/threonine-protein kinase Nek1 isoform X4
XM_011578842.1	XP_011577144.1	serine/threonine-protein kinase Nek1 isoform X6

Pathways from BioSystems
Gene Ontology

The following NEK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAq17588	XM_011578836.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17589	XM_011578838.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17590	XM_011578839.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17591	XM_011578840.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17592	XM_011578841.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17593	XM_011578842.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17594	XM_011578843.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq17595	XM_011578844.1 Latest version!	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEK1 ( XM_011578836.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577138.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq17588
Clone ID Related Accession (Same CDS sequence)	XM_011578836.1
Accession Version	XM_011578836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGGTACAC TGCAAAACGT GGCAGATACC TATGGAAGCA GGCCCATTTC TGCAAGAGGA 
AGGAAATCCA GAAACAAGGA GGAAGAGGAA TATTTGGCAA GATTAAGACA GATCCGGCTA 
CAAAACTTTA ATGAACGTCA ACAGATTAGA GCAAAACTTC GTGGAGAGAA GAATGAAGCT 
GCCAGTTCTG ATCGACAAGA ATCAAGTGAG GAAGCAGAAC TGAAACGCAA AAAGATAGAA 
GCACAGAAGG CCCAAGCAAA TGCTCGAGCT GCAGTTCTGA AAGAACAGTT AGAGCGAAAG 
AGAAAGGAAG CATATGAAAG AGAGAAAAGA GCCTGGGAAG AACATCTGAT AGCGAAAGGG 
GTAAAGGATC CTAATGTGGG AGCTACAGAA CAGAGTCCTG TGCATGGGCT AGAAGAGCTT 
GGAGCAGCTC TTCCTAAGCC ACAAGTTCCA GTGAAACCTG GTACACCAGT CATCTCCATG 
ACTTCTGCTT TGAAGGAAGT GGGTGTGGAT GAAAATGTAA CTGCTATCCA GGAAGCTGAG 
GAGGAAATAA AAAAGCCAGA TTTTGCAATG CAAAACAAAC GAGAAATACT GAGAAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAG AAAGGGAAAA AGGAGTGTAA AAACATCTCT 
GAATTGGCTG GCTCTGAAGA TGGTAAGGAA CAGCACGAAG GTGACCATCC ACTTCTAGGA 
GATCGCAAAA AATGGGAATC TGGGGCATCT GAGTTGGTAG TTCCTCTAGC TCAGTTATCA 
ATGGAAGATT CTCTCTCTGG AACAGACCGT CAAACTCTGG GGGAAGTAAT TAGGTTAGAT 
ATTGGTGAAC CCCACAGGAA AGTCTGGGGC AAAAGCCCTA CTGATTCAGT CCTGAAGATC 
CTAGGAGAAG CAGAGTTGCA GCTGCAAACC GACTTACTTG AGGAACTAGA TGTAATAAAT 
GGTACTGCAG AACTTCTTGA AGGAGATCAT CGGTCCTCAT CAGAGGAGAA GGAAGAGAAA 
GCTTTTCCTA CTGTTGGAGC TCAGTCTTCA GTACCAGTTG GTGTCACAGA GTCTGACATG 
ACTGTACCAG AAGAATCTCA AAGAGCTGGA CCTACCCAGG TGCAGAGCAA GCCTACTATG 
CTGAATGAAC CTGATGATTT GGAAGCAGAG ATGTTGGAAG AAACAGAAGA TAAAAAGCAC 
CAGAGTAAGG AATTTCCCAT CACTGTTAAT GAAGTGTGGG TAAAAGAGAA GGAGGATAAG 
GCACAACATG ACAGGAGAAA CGAGGCAGCT TCTGAGAAAC TAGTGCTTGA CAAGGTGCCA 
CCACAAAAGG AAGCTCCAGA AGAAACTTGT TCCAGTGACT CTCTGCAACC TGAACCCTTA 
TTCCAGAGGG TAATACAGCC CACAGCTGTA CCAACAGCCT CACCAGCTCT GTCAGCTCAG 
TCTTCACAGG AAGAGCCTTT TGTACCTCGT TCACGTTCCA TATCACCAGC AAAAAATAAA 
GGCAAAAGTT CATTGTTGAT TGGACTTTCT ACAGGGCTTT TTGATGCAAA CGACCCAAAG 
ATGTTGAGAA CTTGTTCGCT CCCAGATCTT TACAAACTGT ACAGAACTTT AGTTGATGTC 
CCCAGTGTAG CAGATGTGCA GCATCAACAT AATCTTGAAA TAGATGATAC AGAAGATGAG 
CAAGCCAAAG AAGGACCCTC TGATTCTGAG GATATTATGT TTGGGGAGGC AGATACAGAT 
TTGCAGGAGC TCCGGGCCTC AATGGAACAA TTGCTTAGTG AGCAGCCTAG TGAGGAATTC 
AGTGAAGAGG AGGAGTCTAC TTTAAAGGCT AATGTCATCG AATGCATAAC AAATGGTACA 
GAGCTGGATG AAGGAGACGA AAATAACCCC AGCAGTGAAA GTGCACTTAA TGAAGAATGG 
CAGTCAGATA ATAGTGATGG AGAGATTGCC AGTGAATGTG AAGAATGTGA TAGTATTTTC 
AGCCATTTGG AGGAGTTGAG ATACAACCTT GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
GAGGTTTATG AGAAAATAAA GGCTATACAT GAAGATGAAG ATGAAAATAT TGATATTTGT 
TCAACCATTG TTCAGACTAT TCTTGGAAAT GAACACAAAC ACCTGTATGC CAAAATACTT 
CACCTAGTAA TGGCAGACGG AGCCTATCAA GAAGATAACG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577138.1
CDS	74..3901
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERGIPPGIRPGVPKGPAGHHHLPDAGAVRKKMKRLKEVSKQANANRQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMGTLQNVADTYGSRPISARGRKSRNKEEEEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578836.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578836.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGGTACAC TGCAAAACGT GGCAGATACC TATGGAAGCA GGCCCATTTC TGCAAGAGGA 
AGGAAATCCA GAAACAAGGA GGAAGAGGAA TATTTGGCAA GATTAAGACA GATCCGGCTA 
CAAAACTTTA ATGAACGTCA ACAGATTAGA GCAAAACTTC GTGGAGAGAA GAATGAAGCT 
GCCAGTTCTG ATCGACAAGA ATCAAGTGAG GAAGCAGAAC TGAAACGCAA AAAGATAGAA 
GCACAGAAGG CCCAAGCAAA TGCTCGAGCT GCAGTTCTGA AAGAACAGTT AGAGCGAAAG 
AGAAAGGAAG CATATGAAAG AGAGAAAAGA GCCTGGGAAG AACATCTGAT AGCGAAAGGG 
GTAAAGGATC CTAATGTGGG AGCTACAGAA CAGAGTCCTG TGCATGGGCT AGAAGAGCTT 
GGAGCAGCTC TTCCTAAGCC ACAAGTTCCA GTGAAACCTG GTACACCAGT CATCTCCATG 
ACTTCTGCTT TGAAGGAAGT GGGTGTGGAT GAAAATGTAA CTGCTATCCA GGAAGCTGAG 
GAGGAAATAA AAAAGCCAGA TTTTGCAATG CAAAACAAAC GAGAAATACT GAGAAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAG AAAGGGAAAA AGGAGTGTAA AAACATCTCT 
GAATTGGCTG GCTCTGAAGA TGGTAAGGAA CAGCACGAAG GTGACCATCC ACTTCTAGGA 
GATCGCAAAA AATGGGAATC TGGGGCATCT GAGTTGGTAG TTCCTCTAGC TCAGTTATCA 
ATGGAAGATT CTCTCTCTGG AACAGACCGT CAAACTCTGG GGGAAGTAAT TAGGTTAGAT 
ATTGGTGAAC CCCACAGGAA AGTCTGGGGC AAAAGCCCTA CTGATTCAGT CCTGAAGATC 
CTAGGAGAAG CAGAGTTGCA GCTGCAAACC GACTTACTTG AGGAACTAGA TGTAATAAAT 
GGTACTGCAG AACTTCTTGA AGGAGATCAT CGGTCCTCAT CAGAGGAGAA GGAAGAGAAA 
GCTTTTCCTA CTGTTGGAGC TCAGTCTTCA GTACCAGTTG GTGTCACAGA GTCTGACATG 
ACTGTACCAG AAGAATCTCA AAGAGCTGGA CCTACCCAGG TGCAGAGCAA GCCTACTATG 
CTGAATGAAC CTGATGATTT GGAAGCAGAG ATGTTGGAAG AAACAGAAGA TAAAAAGCAC 
CAGAGTAAGG AATTTCCCAT CACTGTTAAT GAAGTGTGGG TAAAAGAGAA GGAGGATAAG 
GCACAACATG ACAGGAGAAA CGAGGCAGCT TCTGAGAAAC TAGTGCTTGA CAAGGTGCCA 
CCACAAAAGG AAGCTCCAGA AGAAACTTGT TCCAGTGACT CTCTGCAACC TGAACCCTTA 
TTCCAGAGGG TAATACAGCC CACAGCTGTA CCAACAGCCT CACCAGCTCT GTCAGCTCAG 
TCTTCACAGG AAGAGCCTTT TGTACCTCGT TCACGTTCCA TATCACCAGC AAAAAATAAA 
GGCAAAAGTT CATTGTTGAT TGGACTTTCT ACAGGGCTTT TTGATGCAAA CGACCCAAAG 
ATGTTGAGAA CTTGTTCGCT CCCAGATCTT TACAAACTGT ACAGAACTTT AGTTGATGTC 
CCCAGTGTAG CAGATGTGCA GCATCAACAT AATCTTGAAA TAGATGATAC AGAAGATGAG 
CAAGCCAAAG AAGGACCCTC TGATTCTGAG GATATTATGT TTGGGGAGGC AGATACAGAT 
TTGCAGGAGC TCCGGGCCTC AATGGAACAA TTGCTTAGTG AGCAGCCTAG TGAGGAATTC 
AGTGAAGAGG AGGAGTCTAC TTTAAAGGCT AATGTCATCG AATGCATAAC AAATGGTACA 
GAGCTGGATG AAGGAGACGA AAATAACCCC AGCAGTGAAA GTGCACTTAA TGAAGAATGG 
CAGTCAGATA ATAGTGATGG AGAGATTGCC AGTGAATGTG AAGAATGTGA TAGTATTTTC 
AGCCATTTGG AGGAGTTGAG ATACAACCTT GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
GAGGTTTATG AGAAAATAAA GGCTATACAT GAAGATGAAG ATGAAAATAT TGATATTTGT 
TCAACCATTG TTCAGACTAT TCTTGGAAAT GAACACAAAC ACCTGTATGC CAAAATACTT 
CACCTAGTAA TGGCAGACGG AGCCTATCAA GAAGATAACG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578838.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577140.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq17589
Clone ID Related Accession (Same CDS sequence)	XM_011578838.1
Accession Version	XM_011578838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGT CCCTGACCTG GGGTGGAACA TGTGAAAAAA CCATCTTGTT CTGGCTAAGT 
CTAAGGCAAA AAGGATGGAT AGCTGCAGAT AGAGCAAAGC AAGTTGAAGA ATTCTGGCAA 
CGAAAGAGAG AAGCCATGGA AAACAAGGCC CGAGCTGAGG GACACATGGG TACACTGCAA 
AACGTGGCAG ATACCTATGG AAGCAGGCCC ATTTCTGCAA GAGGAAGGAA ATCCAGAAAC 
AAGGAGGAAG AGGAATATTT GGCAAGATTA AGACAGATCC GGCTACAAAA CTTTAATGAA 
CGTCAACAGA TTAGAGCAAA ACTTCGTGGA GAGAAGAATG AAGCTGCCAG TTCTGATCGA 
CAAGAATCAA GTGAGGAAGC AGAACTGAAA CGCAAAAAGA TAGAAGCACA GAAGGCCCAA 
GCAAATGCTC GAGCTGCAGT TCTGAAAGAA CAGTTAGAGC GAAAGAGAAA GGAAGCATAT 
GAAAGAGAGA AAAGAGCCTG GGAAGAACAT CTGATAGCGA AAGGGGTAAA GGATCCTAAT 
GTGGGAGCTA CAGAACAGAG TCCTGTGCAT GGGCTAGAAG AGCTTGGAGC AGCTCTTCCT 
AAGCCACAAG TTCCAGTGAA ACCTGGTACA CCAGTCATCT CCATGACTTC TGCTTTGAAG 
GAAGTGGGTG TGGATGAAAA TGTAACTGCT ATCCAGGAAG CTGAGGAGGA AATAAAAAAG 
CCAGATTTTG CAATGCAAAA CAAACGAGAA ATACTGAGAA GATTAAATCA AAATCTTAAA 
GCTCAAGAAG ATGAGAAAGG GAAAAAGGAG TGTAAAAACA TCTCTGAATT GGCTGGCTCT 
GAAGATGGTA AGGAACAGCA CGAAGGTGAC CATCCACTTC TAGGAGATCG CAAAAAATGG 
GAATCTGGGG CATCTGAGTT GGTAGTTCCT CTAGCTCAGT TATCAATGGA AGATTCTCTC 
TCTGGAACAG ACCGTCAAAC TCTGGGGGAA GTAATTAGGT TAGATATTGG TGAACCCCAC 
AGGAAAGTCT GGGGCAAAAG CCCTACTGAT TCAGTCCTGA AGATCCTAGG AGAAGCAGAG 
TTGCAGCTGC AAACCGACTT ACTTGAGGAA CTAGATGTAA TAAATGGTAC TGCAGAACTT 
CTTGAAGGAG ATCATCGGTC CTCATCAGAG GAGAAGGAAG AGAAAGCTTT TCCTACTGTT 
GGAGCTCAGT CTTCAGTACC AGTTGGTGTC ACAGAGTCTG ACATGACTGT ACCAGAAGAA 
TCTCAAAGAG CTGGACCTAC CCAGGTGCAG AGCAAGCCTA CTATGCTGAA TGAACCTGAT 
GATTTGGAAG CAGAGATGTT GGAAGAAACA GAAGATAAAA AGCACCAGAG TAAGGAATTT 
CCCATCACTG TTAATGAAGT GTGGGTAAAA GAGAAGGAGG ATAAGGCACA ACATGACAGG 
AGAAACGAGG CAGCTTCTGA GAAACTAGTG CTTGACAAGG TGCCACCACA AAAGGAAGCT 
CCAGAAGAAA CTTGTTCCAG TGACTCTCTG CAACCTGAAC CCTTATTCCA GAGGGTAATA 
CAGCCCACAG CTGTACCAAC AGCCTCACCA GCTCTGTCAG CTCAGTCTTC ACAGGAAGAG 
CCTTTTGTAC CTCGTTCACG TTCCATATCA CCAGCAAAAA ATAAAGGCAA AAGTTCATTG 
TTGATTGGAC TTTCTACAGG GCTTTTTGAT GCAAACGACC CAAAGATGTT GAGAACTTGT 
TCGCTCCCAG ATCTTTACAA ACTGTACAGA ACTTTAGTTG ATGTCCCCAG TGTAGCAGAT 
GTGCAGCATC AACATAATCT TGAAATAGAT GATACAGAAG ATGAGCAAGC CAAAGAAGGA 
CCCTCTGATT CTGAGGATAT TATGTTTGGG GAGGCAGATA CAGATTTGCA GGAGCTCCGG 
GCCTCAATGG AACAATTGCT TAGTGAGCAG CCTAGTGAGG AATTCAGTGA AGAGGAGGAG 
TCTACTTTAA AGGCTAATGT CATCGAATGC ATAACAAATG GTACAGAGCT GGATGAAGGA 
GACGAAAATA ACCCCAGCAG TGAAAGTGCA CTTAATGAAG AATGGCAGTC AGATAATAGT 
GATGGAGAGA TTGCCAGTGA ATGTGAAGAA TGTGATAGTA TTTTCAGCCA TTTGGAGGAG 
TTGAGATACA ACCTTGAGCG GGAAATAGGC TTTGATAAAT TCATTGAGGT TTATGAGAAA 
ATAAAGGCTA TACATGAAGA TGAAGATGAA AATATTGATA TTTGTTCAAC CATTGTTCAG 
ACTATTCTTG GAAATGAACA CAAACACCTG TATGCCAAAA TACTTCACCT AGTAATGGCA 
GACGGAGCCT ATCAAGAAGA TAACGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577140.1
CDS	74..3826
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERSLTWGGTCEKTILFWLSLRQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMGTLQNVADTYGSRPISARGRKSRNKEEEEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578838.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578838.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGT CCCTGACCTG GGGTGGAACA TGTGAAAAAA CCATCTTGTT CTGGCTAAGT 
CTAAGGCAAA AAGGATGGAT AGCTGCAGAT AGAGCAAAGC AAGTTGAAGA ATTCTGGCAA 
CGAAAGAGAG AAGCCATGGA AAACAAGGCC CGAGCTGAGG GACACATGGG TACACTGCAA 
AACGTGGCAG ATACCTATGG AAGCAGGCCC ATTTCTGCAA GAGGAAGGAA ATCCAGAAAC 
AAGGAGGAAG AGGAATATTT GGCAAGATTA AGACAGATCC GGCTACAAAA CTTTAATGAA 
CGTCAACAGA TTAGAGCAAA ACTTCGTGGA GAGAAGAATG AAGCTGCCAG TTCTGATCGA 
CAAGAATCAA GTGAGGAAGC AGAACTGAAA CGCAAAAAGA TAGAAGCACA GAAGGCCCAA 
GCAAATGCTC GAGCTGCAGT TCTGAAAGAA CAGTTAGAGC GAAAGAGAAA GGAAGCATAT 
GAAAGAGAGA AAAGAGCCTG GGAAGAACAT CTGATAGCGA AAGGGGTAAA GGATCCTAAT 
GTGGGAGCTA CAGAACAGAG TCCTGTGCAT GGGCTAGAAG AGCTTGGAGC AGCTCTTCCT 
AAGCCACAAG TTCCAGTGAA ACCTGGTACA CCAGTCATCT CCATGACTTC TGCTTTGAAG 
GAAGTGGGTG TGGATGAAAA TGTAACTGCT ATCCAGGAAG CTGAGGAGGA AATAAAAAAG 
CCAGATTTTG CAATGCAAAA CAAACGAGAA ATACTGAGAA GATTAAATCA AAATCTTAAA 
GCTCAAGAAG ATGAGAAAGG GAAAAAGGAG TGTAAAAACA TCTCTGAATT GGCTGGCTCT 
GAAGATGGTA AGGAACAGCA CGAAGGTGAC CATCCACTTC TAGGAGATCG CAAAAAATGG 
GAATCTGGGG CATCTGAGTT GGTAGTTCCT CTAGCTCAGT TATCAATGGA AGATTCTCTC 
TCTGGAACAG ACCGTCAAAC TCTGGGGGAA GTAATTAGGT TAGATATTGG TGAACCCCAC 
AGGAAAGTCT GGGGCAAAAG CCCTACTGAT TCAGTCCTGA AGATCCTAGG AGAAGCAGAG 
TTGCAGCTGC AAACCGACTT ACTTGAGGAA CTAGATGTAA TAAATGGTAC TGCAGAACTT 
CTTGAAGGAG ATCATCGGTC CTCATCAGAG GAGAAGGAAG AGAAAGCTTT TCCTACTGTT 
GGAGCTCAGT CTTCAGTACC AGTTGGTGTC ACAGAGTCTG ACATGACTGT ACCAGAAGAA 
TCTCAAAGAG CTGGACCTAC CCAGGTGCAG AGCAAGCCTA CTATGCTGAA TGAACCTGAT 
GATTTGGAAG CAGAGATGTT GGAAGAAACA GAAGATAAAA AGCACCAGAG TAAGGAATTT 
CCCATCACTG TTAATGAAGT GTGGGTAAAA GAGAAGGAGG ATAAGGCACA ACATGACAGG 
AGAAACGAGG CAGCTTCTGA GAAACTAGTG CTTGACAAGG TGCCACCACA AAAGGAAGCT 
CCAGAAGAAA CTTGTTCCAG TGACTCTCTG CAACCTGAAC CCTTATTCCA GAGGGTAATA 
CAGCCCACAG CTGTACCAAC AGCCTCACCA GCTCTGTCAG CTCAGTCTTC ACAGGAAGAG 
CCTTTTGTAC CTCGTTCACG TTCCATATCA CCAGCAAAAA ATAAAGGCAA AAGTTCATTG 
TTGATTGGAC TTTCTACAGG GCTTTTTGAT GCAAACGACC CAAAGATGTT GAGAACTTGT 
TCGCTCCCAG ATCTTTACAA ACTGTACAGA ACTTTAGTTG ATGTCCCCAG TGTAGCAGAT 
GTGCAGCATC AACATAATCT TGAAATAGAT GATACAGAAG ATGAGCAAGC CAAAGAAGGA 
CCCTCTGATT CTGAGGATAT TATGTTTGGG GAGGCAGATA CAGATTTGCA GGAGCTCCGG 
GCCTCAATGG AACAATTGCT TAGTGAGCAG CCTAGTGAGG AATTCAGTGA AGAGGAGGAG 
TCTACTTTAA AGGCTAATGT CATCGAATGC ATAACAAATG GTACAGAGCT GGATGAAGGA 
GACGAAAATA ACCCCAGCAG TGAAAGTGCA CTTAATGAAG AATGGCAGTC AGATAATAGT 
GATGGAGAGA TTGCCAGTGA ATGTGAAGAA TGTGATAGTA TTTTCAGCCA TTTGGAGGAG 
TTGAGATACA ACCTTGAGCG GGAAATAGGC TTTGATAAAT TCATTGAGGT TTATGAGAAA 
ATAAAGGCTA TACATGAAGA TGAAGATGAA AATATTGATA TTTGTTCAAC CATTGTTCAG 
ACTATTCTTG GAAATGAACA CAAACACCTG TATGCCAAAA TACTTCACCT AGTAATGGCA 
GACGGAGCCT ATCAAGAAGA TAACGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578839.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577141.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OAq17590
Clone ID Related Accession (Same CDS sequence)	XM_011578839.1
Accession Version	XM_011578839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGGCACCA 
TATTATGGCG GTGGAGGTGG TGTTGGTCCT TTTCCTGTGT CTTCCAGAGG ACAATATGAA 
CACTATCATG CTATTTTTGA CCAGATGCAA CAGCAAAAGG AAAACATTAG AGTAGCTGAA 
GAGAGGGAAG CCAAATTGAG GGCGAAAGTA CAAGGCCGAG AAGTTGTTGA AAGAGGAATT 
CCACCTGGAA TACGTCCAGG AGTTCCTAAG GGGCCTGCAG GTCATCACCA TTTACCTGAT 
GCTGGTGCAG TTAGGAAAAA AATGAAAAGA TTGAAGGAGG TGTCTAAACA AGCCAATGCA 
AACAGGCAAA AAGGATGGAT AGCTGCAGAT AGAGCAAAGC AAGTTGAAGA ATTCTGGCAA 
CGAAAGAGAG AAGCCATGGA AAACAAGGCC CGAGCTGAGG GACACATGGG TACACTGCAA 
AACGTGGCAG ATACCTATGG AAGCAGGCCC ATTTCTGCAA GAGGAAGGAA ATCCAGAAAC 
AAGGAGGAAG AGGAATATTT GGCAAGATTA AGACAGATCC GGCTACAAAA CTTTAATGAA 
CGTCAACAGA TTAGAGCAAA ACTTCGTGGA GAGAAGAATG AAGCTGCCAG TTCTGATCGA 
CAAGAATCAA GTGAGGAAGC AGAACTGAAA CGCAAAAAGA TAGAAGCACA GAAGGCCCAA 
GCAAATGCTC GAGCTGCAGT TCTGAAAGAA CAGTTAGAGC GAAAGAGAAA GGAAGCATAT 
GAAAGAGAGA AAAGAGCCTG GGAAGAACAT CTGATAGCGA AAGGGGTAAA GGATCCTAAT 
GTGGGAGCTA CAGAACAGAG TCCTGTGCAT GGGCTAGAAG AGCTTGGAGC AGCTCTTCCT 
AAGCCACAAG TTCCAGTGAA ACCTGGTACA CCAGTCATCT CCATGACTTC TGCTTTGAAG 
GAAGTGGGTG TGGATGAAAA TGTAACTGCT ATCCAGGAAG CTGAGGAGGA AATAAAAAAG 
CCAGATTTTG CAATGCAAAA CAAACGAGAA ATACTGAGAA GATTAAATCA AAATCTTAAA 
GCTCAAGAAG ATGAGAAAGG GAAAAAGGAG TGTAAAAACA TCTCTGAATT GGCTGGCTCT 
GAAGATGGTA AGGAACAGCA CGAAGGTGAC CATCCACTTC TAGGAGATCG CAAAAAATGG 
GAATCTGGGG CATCTGAGTT GGTAGTTCCT CTAGCTCAGT TATCAATGGA AGATTCTCTC 
TCTGGAACAG ACCGTCAAAC TCTGGGGGAA GTAATTAGGT TAGATATTGG TGAACCCCAC 
AGGAAAGTCT GGGGCAAAAG CCCTACTGAT TCAGTCCTGA AGATCCTAGG AGAAGCAGAG 
TTGCAGCTGC AAACCGACTT ACTTGAGGAA CTAGATGTAA TAAATGGTAC TGCAGAACTT 
CTTGAAGGAG ATCATCGGTC CTCATCAGAG GAGAAGGAAG AGAAAGCTTT TCCTACTGTT 
GGAGCTCAGT CTTCAGTACC AGTTGGTGTC ACAGAGTCTG ACATGACTGT ACCAGAAGAA 
TCTCAAAGAG CTGGACCTAC CCAGGTGCAG AGCAAGCCTA CTATGCTGAA TGAACCTGAT 
GATTTGGAAG CAGAGATGTT GGAAGAAACA GAAGATAAAA AGCACCAGAG TAAGGAATTT 
CCCATCACTG TTAATGAAGT GTGGGTAAAA GAGAAGGAGG ATAAGGCACA ACATGACAGG 
AGAAACGAGG CAGCTTCTGA GAAACTAGTG CTTGACAAGG TGCCACCACA AAAGGAAGCT 
CCAGAAGAAA CTTGTTCCAG TGACTCTCTG CAACCTGAAC CCTTATTCCA GAGGGTAATA 
CAGCCCACAG CTGTACCAAC AGCCTCACCA GCTCTGTCAG CTCAGTCTTC ACAGGAAGAG 
CCTTTTGTAC CTCGTTCACG TTCCATATCA CCAGCAAAAA ATAAAGGCAA AAGTTCATTG 
TTGATTGGAC TTTCTACAGG GCTTTTTGAT GCAAACGACC CAAAGATGTT GAGAACTTGT 
TCGCTCCCAG ATCTTTACAA ACTGTACAGA ACTTTAGTTG ATGTCCCCAG TGTAGCAGAT 
GTGCAGCATC AACATAATCT TGAAATAGAT GATACAGAAG ATGAGCAAGC CAAAGAAGGA 
CCCTCTGATT CTGAGGATAT TATGTTTGGG GAGGCAGATA CAGATTTGCA GGAGCTCCGG 
GCCTCAATGG AACAATTGCT TAGTGAGCAG CCTAGTGAGG AATTCAGTGA AGAGGAGGAG 
TCTACTTTAA AGGCTAATGT CATCGAATGC ATAACAAATG GTACAGAGCT GGATGAAGGA 
GACGAAAATA ACCCCAGCAG TGAAAGTGCA CTTAATGAAG AATGGCAGTC AGATAATAGT 
GATGGAGAGA TTGCCAGTGA ATGTGAAGAA TGTGATAGTA TTTTCAGCCA TTTGGAGGAG 
TTGAGATACA ACCTTGAGCG GGAAATAGGC TTTGATAAAT TCATTGAGGT TTATGAGAAA 
ATAAAGGCTA TACATGAAGA TGAAGATGAA AATATTGATA TTTGTTCAAC CATTGTTCAG 
ACTATTCTTG GAAATGAACA CAAACACCTG TATGCCAAAA TACTTCACCT AGTAATGGCA 
GACGGAGCCT ATCAAGAAGA TAACGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577141.1
CDS	74..3826
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERGIPPGIRPGVPKGPAGHHHLPDAGAVRKKMKRLKEVSKQANANRQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMGTLQNVADTYGSRPISARGRKSRNKEEEEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578839.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578839.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGGCACCA 
TATTATGGCG GTGGAGGTGG TGTTGGTCCT TTTCCTGTGT CTTCCAGAGG ACAATATGAA 
CACTATCATG CTATTTTTGA CCAGATGCAA CAGCAAAAGG AAAACATTAG AGTAGCTGAA 
GAGAGGGAAG CCAAATTGAG GGCGAAAGTA CAAGGCCGAG AAGTTGTTGA AAGAGGAATT 
CCACCTGGAA TACGTCCAGG AGTTCCTAAG GGGCCTGCAG GTCATCACCA TTTACCTGAT 
GCTGGTGCAG TTAGGAAAAA AATGAAAAGA TTGAAGGAGG TGTCTAAACA AGCCAATGCA 
AACAGGCAAA AAGGATGGAT AGCTGCAGAT AGAGCAAAGC AAGTTGAAGA ATTCTGGCAA 
CGAAAGAGAG AAGCCATGGA AAACAAGGCC CGAGCTGAGG GACACATGGG TACACTGCAA 
AACGTGGCAG ATACCTATGG AAGCAGGCCC ATTTCTGCAA GAGGAAGGAA ATCCAGAAAC 
AAGGAGGAAG AGGAATATTT GGCAAGATTA AGACAGATCC GGCTACAAAA CTTTAATGAA 
CGTCAACAGA TTAGAGCAAA ACTTCGTGGA GAGAAGAATG AAGCTGCCAG TTCTGATCGA 
CAAGAATCAA GTGAGGAAGC AGAACTGAAA CGCAAAAAGA TAGAAGCACA GAAGGCCCAA 
GCAAATGCTC GAGCTGCAGT TCTGAAAGAA CAGTTAGAGC GAAAGAGAAA GGAAGCATAT 
GAAAGAGAGA AAAGAGCCTG GGAAGAACAT CTGATAGCGA AAGGGGTAAA GGATCCTAAT 
GTGGGAGCTA CAGAACAGAG TCCTGTGCAT GGGCTAGAAG AGCTTGGAGC AGCTCTTCCT 
AAGCCACAAG TTCCAGTGAA ACCTGGTACA CCAGTCATCT CCATGACTTC TGCTTTGAAG 
GAAGTGGGTG TGGATGAAAA TGTAACTGCT ATCCAGGAAG CTGAGGAGGA AATAAAAAAG 
CCAGATTTTG CAATGCAAAA CAAACGAGAA ATACTGAGAA GATTAAATCA AAATCTTAAA 
GCTCAAGAAG ATGAGAAAGG GAAAAAGGAG TGTAAAAACA TCTCTGAATT GGCTGGCTCT 
GAAGATGGTA AGGAACAGCA CGAAGGTGAC CATCCACTTC TAGGAGATCG CAAAAAATGG 
GAATCTGGGG CATCTGAGTT GGTAGTTCCT CTAGCTCAGT TATCAATGGA AGATTCTCTC 
TCTGGAACAG ACCGTCAAAC TCTGGGGGAA GTAATTAGGT TAGATATTGG TGAACCCCAC 
AGGAAAGTCT GGGGCAAAAG CCCTACTGAT TCAGTCCTGA AGATCCTAGG AGAAGCAGAG 
TTGCAGCTGC AAACCGACTT ACTTGAGGAA CTAGATGTAA TAAATGGTAC TGCAGAACTT 
CTTGAAGGAG ATCATCGGTC CTCATCAGAG GAGAAGGAAG AGAAAGCTTT TCCTACTGTT 
GGAGCTCAGT CTTCAGTACC AGTTGGTGTC ACAGAGTCTG ACATGACTGT ACCAGAAGAA 
TCTCAAAGAG CTGGACCTAC CCAGGTGCAG AGCAAGCCTA CTATGCTGAA TGAACCTGAT 
GATTTGGAAG CAGAGATGTT GGAAGAAACA GAAGATAAAA AGCACCAGAG TAAGGAATTT 
CCCATCACTG TTAATGAAGT GTGGGTAAAA GAGAAGGAGG ATAAGGCACA ACATGACAGG 
AGAAACGAGG CAGCTTCTGA GAAACTAGTG CTTGACAAGG TGCCACCACA AAAGGAAGCT 
CCAGAAGAAA CTTGTTCCAG TGACTCTCTG CAACCTGAAC CCTTATTCCA GAGGGTAATA 
CAGCCCACAG CTGTACCAAC AGCCTCACCA GCTCTGTCAG CTCAGTCTTC ACAGGAAGAG 
CCTTTTGTAC CTCGTTCACG TTCCATATCA CCAGCAAAAA ATAAAGGCAA AAGTTCATTG 
TTGATTGGAC TTTCTACAGG GCTTTTTGAT GCAAACGACC CAAAGATGTT GAGAACTTGT 
TCGCTCCCAG ATCTTTACAA ACTGTACAGA ACTTTAGTTG ATGTCCCCAG TGTAGCAGAT 
GTGCAGCATC AACATAATCT TGAAATAGAT GATACAGAAG ATGAGCAAGC CAAAGAAGGA 
CCCTCTGATT CTGAGGATAT TATGTTTGGG GAGGCAGATA CAGATTTGCA GGAGCTCCGG 
GCCTCAATGG AACAATTGCT TAGTGAGCAG CCTAGTGAGG AATTCAGTGA AGAGGAGGAG 
TCTACTTTAA AGGCTAATGT CATCGAATGC ATAACAAATG GTACAGAGCT GGATGAAGGA 
GACGAAAATA ACCCCAGCAG TGAAAGTGCA CTTAATGAAG AATGGCAGTC AGATAATAGT 
GATGGAGAGA TTGCCAGTGA ATGTGAAGAA TGTGATAGTA TTTTCAGCCA TTTGGAGGAG 
TTGAGATACA ACCTTGAGCG GGAAATAGGC TTTGATAAAT TCATTGAGGT TTATGAGAAA 
ATAAAGGCTA TACATGAAGA TGAAGATGAA AATATTGATA TTTGTTCAAC CATTGTTCAG 
ACTATTCTTG GAAATGAACA CAAACACCTG TATGCCAAAA TACTTCACCT AGTAATGGCA 
GACGGAGCCT ATCAAGAAGA TAACGATGAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578840.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577142.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAq17591
Clone ID Related Accession (Same CDS sequence)	XM_011578840.1
Accession Version	XM_011578840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGAATATT TGGCAAGATT AAGACAGATC CGGCTACAAA ACTTTAATGA ACGTCAACAG 
ATTAGAGCAA AACTTCGTGG AGAGAAGAAT GAAGCTGCCA GTTCTGATCG ACAAGAATCA 
AGTGAGGAAG CAGAACTGAA ACGCAAAAAG ATAGAAGCAC AGAAGGCCCA AGCAAATGCT 
CGAGCTGCAG TTCTGAAAGA ACAGTTAGAG CGAAAGAGAA AGGAAGCATA TGAAAGAGAG 
AAAAGAGCCT GGGAAGAACA TCTGATAGCG AAAGGGGTAA AGGATCCTAA TGTGGGAGCT 
ACAGAACAGA GTCCTGTGCA TGGGCTAGAA GAGCTTGGAG CAGCTCTTCC TAAGCCACAA 
GTTCCAGTGA AACCTGGTAC ACCAGTCATC TCCATGACTT CTGCTTTGAA GGAAGTGGGT 
GTGGATGAAA ATGTAACTGC TATCCAGGAA GCTGAGGAGG AAATAAAAAA GCCAGATTTT 
GCAATGCAAA ACAAACGAGA AATACTGAGA AGATTAAATC AAAATCTTAA AGCTCAAGAA 
GATGAGAAAG GGAAAAAGGA GTGTAAAAAC ATCTCTGAAT TGGCTGGCTC TGAAGATGGT 
AAGGAACAGC ACGAAGGTGA CCATCCACTT CTAGGAGATC GCAAAAAATG GGAATCTGGG 
GCATCTGAGT TGGTAGTTCC TCTAGCTCAG TTATCAATGG AAGATTCTCT CTCTGGAACA 
GACCGTCAAA CTCTGGGGGA AGTAATTAGG TTAGATATTG GTGAACCCCA CAGGAAAGTC 
TGGGGCAAAA GCCCTACTGA TTCAGTCCTG AAGATCCTAG GAGAAGCAGA GTTGCAGCTG 
CAAACCGACT TACTTGAGGA ACTAGATGTA ATAAATGGTA CTGCAGAACT TCTTGAAGGA 
GATCATCGGT CCTCATCAGA GGAGAAGGAA GAGAAAGCTT TTCCTACTGT TGGAGCTCAG 
TCTTCAGTAC CAGTTGGTGT CACAGAGTCT GACATGACTG TACCAGAAGA ATCTCAAAGA 
GCTGGACCTA CCCAGGTGCA GAGCAAGCCT ACTATGCTGA ATGAACCTGA TGATTTGGAA 
GCAGAGATGT TGGAAGAAAC AGAAGATAAA AAGCACCAGA GTAAGGAATT TCCCATCACT 
GTTAATGAAG TGTGGGTAAA AGAGAAGGAG GATAAGGCAC AACATGACAG GAGAAACGAG 
GCAGCTTCTG AGAAACTAGT GCTTGACAAG GTGCCACCAC AAAAGGAAGC TCCAGAAGAA 
ACTTGTTCCA GTGACTCTCT GCAACCTGAA CCCTTATTCC AGAGGGTAAT ACAGCCCACA 
GCTGTACCAA CAGCCTCACC AGCTCTGTCA GCTCAGTCTT CACAGGAAGA GCCTTTTGTA 
CCTCGTTCAC GTTCCATATC ACCAGCAAAA AATAAAGGCA AAAGTTCATT GTTGATTGGA 
CTTTCTACAG GGCTTTTTGA TGCAAACGAC CCAAAGATGT TGAGAACTTG TTCGCTCCCA 
GATCTTTACA AACTGTACAG AACTTTAGTT GATGTCCCCA GTGTAGCAGA TGTGCAGCAT 
CAACATAATC TTGAAATAGA TGATACAGAA GATGAGCAAG CCAAAGAAGG ACCCTCTGAT 
TCTGAGGATA TTATGTTTGG GGAGGCAGAT ACAGATTTGC AGGAGCTCCG GGCCTCAATG 
GAACAATTGC TTAGTGAGCA GCCTAGTGAG GAATTCAGTG AAGAGGAGGA GTCTACTTTA 
AAGGCTAATG TCATCGAATG CATAACAAAT GGTACAGAGC TGGATGAAGG AGACGAAAAT 
AACCCCAGCA GTGAAAGTGC ACTTAATGAA GAATGGCAGT CAGATAATAG TGATGGAGAG 
ATTGCCAGTG AATGTGAAGA ATGTGATAGT ATTTTCAGCC ATTTGGAGGA GTTGAGATAC 
AACCTTGAGC GGGAAATAGG CTTTGATAAA TTCATTGAGG TTTATGAGAA AATAAAGGCT 
ATACATGAAG ATGAAGATGA AAATATTGAT ATTTGTTCAA CCATTGTTCA GACTATTCTT 
GGAAATGAAC ACAAACACCT GTATGCCAAA ATACTTCACC TAGTAATGGC AGACGGAGCC 
TATCAAGAAG ATAACGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577142.1
CDS	74..3817
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERGIPPGIRPGVPKGPAGHHHLPDAGAVRKKMKRLKEVSKQANANRQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578840.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578840.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGAATATT TGGCAAGATT AAGACAGATC CGGCTACAAA ACTTTAATGA ACGTCAACAG 
ATTAGAGCAA AACTTCGTGG AGAGAAGAAT GAAGCTGCCA GTTCTGATCG ACAAGAATCA 
AGTGAGGAAG CAGAACTGAA ACGCAAAAAG ATAGAAGCAC AGAAGGCCCA AGCAAATGCT 
CGAGCTGCAG TTCTGAAAGA ACAGTTAGAG CGAAAGAGAA AGGAAGCATA TGAAAGAGAG 
AAAAGAGCCT GGGAAGAACA TCTGATAGCG AAAGGGGTAA AGGATCCTAA TGTGGGAGCT 
ACAGAACAGA GTCCTGTGCA TGGGCTAGAA GAGCTTGGAG CAGCTCTTCC TAAGCCACAA 
GTTCCAGTGA AACCTGGTAC ACCAGTCATC TCCATGACTT CTGCTTTGAA GGAAGTGGGT 
GTGGATGAAA ATGTAACTGC TATCCAGGAA GCTGAGGAGG AAATAAAAAA GCCAGATTTT 
GCAATGCAAA ACAAACGAGA AATACTGAGA AGATTAAATC AAAATCTTAA AGCTCAAGAA 
GATGAGAAAG GGAAAAAGGA GTGTAAAAAC ATCTCTGAAT TGGCTGGCTC TGAAGATGGT 
AAGGAACAGC ACGAAGGTGA CCATCCACTT CTAGGAGATC GCAAAAAATG GGAATCTGGG 
GCATCTGAGT TGGTAGTTCC TCTAGCTCAG TTATCAATGG AAGATTCTCT CTCTGGAACA 
GACCGTCAAA CTCTGGGGGA AGTAATTAGG TTAGATATTG GTGAACCCCA CAGGAAAGTC 
TGGGGCAAAA GCCCTACTGA TTCAGTCCTG AAGATCCTAG GAGAAGCAGA GTTGCAGCTG 
CAAACCGACT TACTTGAGGA ACTAGATGTA ATAAATGGTA CTGCAGAACT TCTTGAAGGA 
GATCATCGGT CCTCATCAGA GGAGAAGGAA GAGAAAGCTT TTCCTACTGT TGGAGCTCAG 
TCTTCAGTAC CAGTTGGTGT CACAGAGTCT GACATGACTG TACCAGAAGA ATCTCAAAGA 
GCTGGACCTA CCCAGGTGCA GAGCAAGCCT ACTATGCTGA ATGAACCTGA TGATTTGGAA 
GCAGAGATGT TGGAAGAAAC AGAAGATAAA AAGCACCAGA GTAAGGAATT TCCCATCACT 
GTTAATGAAG TGTGGGTAAA AGAGAAGGAG GATAAGGCAC AACATGACAG GAGAAACGAG 
GCAGCTTCTG AGAAACTAGT GCTTGACAAG GTGCCACCAC AAAAGGAAGC TCCAGAAGAA 
ACTTGTTCCA GTGACTCTCT GCAACCTGAA CCCTTATTCC AGAGGGTAAT ACAGCCCACA 
GCTGTACCAA CAGCCTCACC AGCTCTGTCA GCTCAGTCTT CACAGGAAGA GCCTTTTGTA 
CCTCGTTCAC GTTCCATATC ACCAGCAAAA AATAAAGGCA AAAGTTCATT GTTGATTGGA 
CTTTCTACAG GGCTTTTTGA TGCAAACGAC CCAAAGATGT TGAGAACTTG TTCGCTCCCA 
GATCTTTACA AACTGTACAG AACTTTAGTT GATGTCCCCA GTGTAGCAGA TGTGCAGCAT 
CAACATAATC TTGAAATAGA TGATACAGAA GATGAGCAAG CCAAAGAAGG ACCCTCTGAT 
TCTGAGGATA TTATGTTTGG GGAGGCAGAT ACAGATTTGC AGGAGCTCCG GGCCTCAATG 
GAACAATTGC TTAGTGAGCA GCCTAGTGAG GAATTCAGTG AAGAGGAGGA GTCTACTTTA 
AAGGCTAATG TCATCGAATG CATAACAAAT GGTACAGAGC TGGATGAAGG AGACGAAAAT 
AACCCCAGCA GTGAAAGTGC ACTTAATGAA GAATGGCAGT CAGATAATAG TGATGGAGAG 
ATTGCCAGTG AATGTGAAGA ATGTGATAGT ATTTTCAGCC ATTTGGAGGA GTTGAGATAC 
AACCTTGAGC GGGAAATAGG CTTTGATAAA TTCATTGAGG TTTATGAGAA AATAAAGGCT 
ATACATGAAG ATGAAGATGA AAATATTGAT ATTTGTTCAA CCATTGTTCA GACTATTCTT 
GGAAATGAAC ACAAACACCT GTATGCCAAA ATACTTCACC TAGTAATGGC AGACGGAGCC 
TATCAAGAAG ATAACGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578841.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577143.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAq17592
Clone ID Related Accession (Same CDS sequence)	XM_011578841.1
Accession Version	XM_011578841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGC AAAAAGGATG GATAGCTGCA GATAGAGCAA AGCAAGTTGA AGAATTCTGG 
CAACGAAAGA GAGAAGCCAT GGAAAACAAG GCCCGAGCTG AGGGACACAT GGGTACACTG 
CAAAACGTGG CAGATACCTA TGGAAGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
AACAAGGAGG AAGAGGAATA TTTGGCAAGA TTAAGACAGA TCCGGCTACA AAACTTTAAT 
GAACGTCAAC AGATTAGAGC AAAACTTCGT GGAGAGAAGA ATGAAGCTGC CAGTTCTGAT 
CGACAAGAAT CAAGTGAGGA AGCAGAACTG AAACGCAAAA AGATAGAAGC ACAGAAGGCC 
CAAGCAAATG CTCGAGCTGC AGTTCTGAAA GAACAGTTAG AGCGAAAGAG AAAGGAAGCA 
TATGAAAGAG AGAAAAGAGC CTGGGAAGAA CATCTGATAG CGAAAGGGGT AAAGGATCCT 
AATGTGGGAG CTACAGAACA GAGTCCTGTG CATGGGCTAG AAGAGCTTGG AGCAGCTCTT 
CCTAAGCCAC AAGTTCCAGT GAAACCTGGT ACACCAGTCA TCTCCATGAC TTCTGCTTTG 
AAGGAAGTGG GTGTGGATGA AAATGTAACT GCTATCCAGG AAGCTGAGGA GGAAATAAAA 
AAGCCAGATT TTGCAATGCA AAACAAACGA GAAATACTGA GAAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAGAA AGGGAAAAAG GAGTGTAAAA ACATCTCTGA ATTGGCTGGC 
TCTGAAGATG GTAAGGAACA GCACGAAGGT GACCATCCAC TTCTAGGAGA TCGCAAAAAA 
TGGGAATCTG GGGCATCTGA GTTGGTAGTT CCTCTAGCTC AGTTATCAAT GGAAGATTCT 
CTCTCTGGAA CAGACCGTCA AACTCTGGGG GAAGTAATTA GGTTAGATAT TGGTGAACCC 
CACAGGAAAG TCTGGGGCAA AAGCCCTACT GATTCAGTCC TGAAGATCCT AGGAGAAGCA 
GAGTTGCAGC TGCAAACCGA CTTACTTGAG GAACTAGATG TAATAAATGG TACTGCAGAA 
CTTCTTGAAG GAGATCATCG GTCCTCATCA GAGGAGAAGG AAGAGAAAGC TTTTCCTACT 
GTTGGAGCTC AGTCTTCAGT ACCAGTTGGT GTCACAGAGT CTGACATGAC TGTACCAGAA 
GAATCTCAAA GAGCTGGACC TACCCAGGTG CAGAGCAAGC CTACTATGCT GAATGAACCT 
GATGATTTGG AAGCAGAGAT GTTGGAAGAA ACAGAAGATA AAAAGCACCA GAGTAAGGAA 
TTTCCCATCA CTGTTAATGA AGTGTGGGTA AAAGAGAAGG AGGATAAGGC ACAACATGAC 
AGGAGAAACG AGGCAGCTTC TGAGAAACTA GTGCTTGACA AGGTGCCACC ACAAAAGGAA 
GCTCCAGAAG AAACTTGTTC CAGTGACTCT CTGCAACCTG AACCCTTATT CCAGAGGGTA 
ATACAGCCCA CAGCTGTACC AACAGCCTCA CCAGCTCTGT CAGCTCAGTC TTCACAGGAA 
GAGCCTTTTG TACCTCGTTC ACGTTCCATA TCACCAGCAA AAAATAAAGG CAAAAGTTCA 
TTGTTGATTG GACTTTCTAC AGGGCTTTTT GATGCAAACG ACCCAAAGAT GTTGAGAACT 
TGTTCGCTCC CAGATCTTTA CAAACTGTAC AGAACTTTAG TTGATGTCCC CAGTGTAGCA 
GATGTGCAGC ATCAACATAA TCTTGAAATA GATGATACAG AAGATGAGCA AGCCAAAGAA 
GGACCCTCTG ATTCTGAGGA TATTATGTTT GGGGAGGCAG ATACAGATTT GCAGGAGCTC 
CGGGCCTCAA TGGAACAATT GCTTAGTGAG CAGCCTAGTG AGGAATTCAG TGAAGAGGAG 
GAGTCTACTT TAAAGGCTAA TGTCATCGAA TGCATAACAA ATGGTACAGA GCTGGATGAA 
GGAGACGAAA ATAACCCCAG CAGTGAAAGT GCACTTAATG AAGAATGGCA GTCAGATAAT 
AGTGATGGAG AGATTGCCAG TGAATGTGAA GAATGTGATA GTATTTTCAG CCATTTGGAG 
GAGTTGAGAT ACAACCTTGA GCGGGAAATA GGCTTTGATA AATTCATTGA GGTTTATGAG 
AAAATAAAGG CTATACATGA AGATGAAGAT GAAAATATTG ATATTTGTTC AACCATTGTT 
CAGACTATTC TTGGAAATGA ACACAAACAC CTGTATGCCA AAATACTTCA CCTAGTAATG 
GCAGACGGAG CCTATCAAGA AGATAACGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577143.1
CDS	74..3769
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMGTLQNVADTYGSRPISARGRKSRNKEEEEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578841.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578841.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGC AAAAAGGATG GATAGCTGCA GATAGAGCAA AGCAAGTTGA AGAATTCTGG 
CAACGAAAGA GAGAAGCCAT GGAAAACAAG GCCCGAGCTG AGGGACACAT GGGTACACTG 
CAAAACGTGG CAGATACCTA TGGAAGCAGG CCCATTTCTG CAAGAGGAAG GAAATCCAGA 
AACAAGGAGG AAGAGGAATA TTTGGCAAGA TTAAGACAGA TCCGGCTACA AAACTTTAAT 
GAACGTCAAC AGATTAGAGC AAAACTTCGT GGAGAGAAGA ATGAAGCTGC CAGTTCTGAT 
CGACAAGAAT CAAGTGAGGA AGCAGAACTG AAACGCAAAA AGATAGAAGC ACAGAAGGCC 
CAAGCAAATG CTCGAGCTGC AGTTCTGAAA GAACAGTTAG AGCGAAAGAG AAAGGAAGCA 
TATGAAAGAG AGAAAAGAGC CTGGGAAGAA CATCTGATAG CGAAAGGGGT AAAGGATCCT 
AATGTGGGAG CTACAGAACA GAGTCCTGTG CATGGGCTAG AAGAGCTTGG AGCAGCTCTT 
CCTAAGCCAC AAGTTCCAGT GAAACCTGGT ACACCAGTCA TCTCCATGAC TTCTGCTTTG 
AAGGAAGTGG GTGTGGATGA AAATGTAACT GCTATCCAGG AAGCTGAGGA GGAAATAAAA 
AAGCCAGATT TTGCAATGCA AAACAAACGA GAAATACTGA GAAGATTAAA TCAAAATCTT 
AAAGCTCAAG AAGATGAGAA AGGGAAAAAG GAGTGTAAAA ACATCTCTGA ATTGGCTGGC 
TCTGAAGATG GTAAGGAACA GCACGAAGGT GACCATCCAC TTCTAGGAGA TCGCAAAAAA 
TGGGAATCTG GGGCATCTGA GTTGGTAGTT CCTCTAGCTC AGTTATCAAT GGAAGATTCT 
CTCTCTGGAA CAGACCGTCA AACTCTGGGG GAAGTAATTA GGTTAGATAT TGGTGAACCC 
CACAGGAAAG TCTGGGGCAA AAGCCCTACT GATTCAGTCC TGAAGATCCT AGGAGAAGCA 
GAGTTGCAGC TGCAAACCGA CTTACTTGAG GAACTAGATG TAATAAATGG TACTGCAGAA 
CTTCTTGAAG GAGATCATCG GTCCTCATCA GAGGAGAAGG AAGAGAAAGC TTTTCCTACT 
GTTGGAGCTC AGTCTTCAGT ACCAGTTGGT GTCACAGAGT CTGACATGAC TGTACCAGAA 
GAATCTCAAA GAGCTGGACC TACCCAGGTG CAGAGCAAGC CTACTATGCT GAATGAACCT 
GATGATTTGG AAGCAGAGAT GTTGGAAGAA ACAGAAGATA AAAAGCACCA GAGTAAGGAA 
TTTCCCATCA CTGTTAATGA AGTGTGGGTA AAAGAGAAGG AGGATAAGGC ACAACATGAC 
AGGAGAAACG AGGCAGCTTC TGAGAAACTA GTGCTTGACA AGGTGCCACC ACAAAAGGAA 
GCTCCAGAAG AAACTTGTTC CAGTGACTCT CTGCAACCTG AACCCTTATT CCAGAGGGTA 
ATACAGCCCA CAGCTGTACC AACAGCCTCA CCAGCTCTGT CAGCTCAGTC TTCACAGGAA 
GAGCCTTTTG TACCTCGTTC ACGTTCCATA TCACCAGCAA AAAATAAAGG CAAAAGTTCA 
TTGTTGATTG GACTTTCTAC AGGGCTTTTT GATGCAAACG ACCCAAAGAT GTTGAGAACT 
TGTTCGCTCC CAGATCTTTA CAAACTGTAC AGAACTTTAG TTGATGTCCC CAGTGTAGCA 
GATGTGCAGC ATCAACATAA TCTTGAAATA GATGATACAG AAGATGAGCA AGCCAAAGAA 
GGACCCTCTG ATTCTGAGGA TATTATGTTT GGGGAGGCAG ATACAGATTT GCAGGAGCTC 
CGGGCCTCAA TGGAACAATT GCTTAGTGAG CAGCCTAGTG AGGAATTCAG TGAAGAGGAG 
GAGTCTACTT TAAAGGCTAA TGTCATCGAA TGCATAACAA ATGGTACAGA GCTGGATGAA 
GGAGACGAAA ATAACCCCAG CAGTGAAAGT GCACTTAATG AAGAATGGCA GTCAGATAAT 
AGTGATGGAG AGATTGCCAG TGAATGTGAA GAATGTGATA GTATTTTCAG CCATTTGGAG 
GAGTTGAGAT ACAACCTTGA GCGGGAAATA GGCTTTGATA AATTCATTGA GGTTTATGAG 
AAAATAAAGG CTATACATGA AGATGAAGAT GAAAATATTG ATATTTGTTC AACCATTGTT 
CAGACTATTC TTGGAAATGA ACACAAACAC CTGTATGCCA AAATACTTCA CCTAGTAATG 
GCAGACGGAG CCTATCAAGA AGATAACGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578842.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577144.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

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CloneID	OAq17593
Clone ID Related Accession (Same CDS sequence)	XM_011578842.1
Accession Version	XM_011578842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGC AAAAAGGATG GATAGCTGCA GATAGAGCAA AGCAAGTTGA AGAATTCTGG 
CAACGAAAGA GAGAAGCCAT GGAAAACAAG GCCCGAGCTG AGGGACACAT GGAATATTTG 
GCAAGATTAA GACAGATCCG GCTACAAAAC TTTAATGAAC GTCAACAGAT TAGAGCAAAA 
CTTCGTGGAG AGAAGAATGA AGCTGCCAGT TCTGATCGAC AAGAATCAAG TGAGGAAGCA 
GAACTGAAAC GCAAAAAGAT AGAAGCACAG AAGGCCCAAG CAAATGCTCG AGCTGCAGTT 
CTGAAAGAAC AGTTAGAGCG AAAGAGAAAG GAAGCATATG AAAGAGAGAA AAGAGCCTGG 
GAAGAACATC TGATAGCGAA AGGGGTAAAG GATCCTAATG TGGGAGCTAC AGAACAGAGT 
CCTGTGCATG GGCTAGAAGA GCTTGGAGCA GCTCTTCCTA AGCCACAAGT TCCAGTGAAA 
CCTGGTACAC CAGTCATCTC CATGACTTCT GCTTTGAAGG AAGTGGGTGT GGATGAAAAT 
GTAACTGCTA TCCAGGAAGC TGAGGAGGAA ATAAAAAAGC CAGATTTTGC AATGCAAAAC 
AAACGAGAAA TACTGAGAAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAGAAAGGG 
AAAAAGGAGT GTAAAAACAT CTCTGAATTG GCTGGCTCTG AAGATGGTAA GGAACAGCAC 
GAAGGTGACC ATCCACTTCT AGGAGATCGC AAAAAATGGG AATCTGGGGC ATCTGAGTTG 
GTAGTTCCTC TAGCTCAGTT ATCAATGGAA GATTCTCTCT CTGGAACAGA CCGTCAAACT 
CTGGGGGAAG TAATTAGGTT AGATATTGGT GAACCCCACA GGAAAGTCTG GGGCAAAAGC 
CCTACTGATT CAGTCCTGAA GATCCTAGGA GAAGCAGAGT TGCAGCTGCA AACCGACTTA 
CTTGAGGAAC TAGATGTAAT AAATGGTACT GCAGAACTTC TTGAAGGAGA TCATCGGTCC 
TCATCAGAGG AGAAGGAAGA GAAAGCTTTT CCTACTGTTG GAGCTCAGTC TTCAGTACCA 
GTTGGTGTCA CAGAGTCTGA CATGACTGTA CCAGAAGAAT CTCAAAGAGC TGGACCTACC 
CAGGTGCAGA GCAAGCCTAC TATGCTGAAT GAACCTGATG ATTTGGAAGC AGAGATGTTG 
GAAGAAACAG AAGATAAAAA GCACCAGAGT AAGGAATTTC CCATCACTGT TAATGAAGTG 
TGGGTAAAAG AGAAGGAGGA TAAGGCACAA CATGACAGGA GAAACGAGGC AGCTTCTGAG 
AAACTAGTGC TTGACAAGGT GCCACCACAA AAGGAAGCTC CAGAAGAAAC TTGTTCCAGT 
GACTCTCTGC AACCTGAACC CTTATTCCAG AGGGTAATAC AGCCCACAGC TGTACCAACA 
GCCTCACCAG CTCTGTCAGC TCAGTCTTCA CAGGAAGAGC CTTTTGTACC TCGTTCACGT 
TCCATATCAC CAGCAAAAAA TAAAGGCAAA AGTTCATTGT TGATTGGACT TTCTACAGGG 
CTTTTTGATG CAAACGACCC AAAGATGTTG AGAACTTGTT CGCTCCCAGA TCTTTACAAA 
CTGTACAGAA CTTTAGTTGA TGTCCCCAGT GTAGCAGATG TGCAGCATCA ACATAATCTT 
GAAATAGATG ATACAGAAGA TGAGCAAGCC AAAGAAGGAC CCTCTGATTC TGAGGATATT 
ATGTTTGGGG AGGCAGATAC AGATTTGCAG GAGCTCCGGG CCTCAATGGA ACAATTGCTT 
AGTGAGCAGC CTAGTGAGGA ATTCAGTGAA GAGGAGGAGT CTACTTTAAA GGCTAATGTC 
ATCGAATGCA TAACAAATGG TACAGAGCTG GATGAAGGAG ACGAAAATAA CCCCAGCAGT 
GAAAGTGCAC TTAATGAAGA ATGGCAGTCA GATAATAGTG ATGGAGAGAT TGCCAGTGAA 
TGTGAAGAAT GTGATAGTAT TTTCAGCCAT TTGGAGGAGT TGAGATACAA CCTTGAGCGG 
GAAATAGGCT TTGATAAATT CATTGAGGTT TATGAGAAAA TAAAGGCTAT ACATGAAGAT 
GAAGATGAAA ATATTGATAT TTGTTCAACC ATTGTTCAGA CTATTCTTGG AAATGAACAC 
AAACACCTGT ATGCCAAAAT ACTTCACCTA GTAATGGCAG ACGGAGCCTA TCAAGAAGAT 
AACGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577144.1
CDS	74..3685
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578842.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578842.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGGC AAAAAGGATG GATAGCTGCA GATAGAGCAA AGCAAGTTGA AGAATTCTGG 
CAACGAAAGA GAGAAGCCAT GGAAAACAAG GCCCGAGCTG AGGGACACAT GGAATATTTG 
GCAAGATTAA GACAGATCCG GCTACAAAAC TTTAATGAAC GTCAACAGAT TAGAGCAAAA 
CTTCGTGGAG AGAAGAATGA AGCTGCCAGT TCTGATCGAC AAGAATCAAG TGAGGAAGCA 
GAACTGAAAC GCAAAAAGAT AGAAGCACAG AAGGCCCAAG CAAATGCTCG AGCTGCAGTT 
CTGAAAGAAC AGTTAGAGCG AAAGAGAAAG GAAGCATATG AAAGAGAGAA AAGAGCCTGG 
GAAGAACATC TGATAGCGAA AGGGGTAAAG GATCCTAATG TGGGAGCTAC AGAACAGAGT 
CCTGTGCATG GGCTAGAAGA GCTTGGAGCA GCTCTTCCTA AGCCACAAGT TCCAGTGAAA 
CCTGGTACAC CAGTCATCTC CATGACTTCT GCTTTGAAGG AAGTGGGTGT GGATGAAAAT 
GTAACTGCTA TCCAGGAAGC TGAGGAGGAA ATAAAAAAGC CAGATTTTGC AATGCAAAAC 
AAACGAGAAA TACTGAGAAG ATTAAATCAA AATCTTAAAG CTCAAGAAGA TGAGAAAGGG 
AAAAAGGAGT GTAAAAACAT CTCTGAATTG GCTGGCTCTG AAGATGGTAA GGAACAGCAC 
GAAGGTGACC ATCCACTTCT AGGAGATCGC AAAAAATGGG AATCTGGGGC ATCTGAGTTG 
GTAGTTCCTC TAGCTCAGTT ATCAATGGAA GATTCTCTCT CTGGAACAGA CCGTCAAACT 
CTGGGGGAAG TAATTAGGTT AGATATTGGT GAACCCCACA GGAAAGTCTG GGGCAAAAGC 
CCTACTGATT CAGTCCTGAA GATCCTAGGA GAAGCAGAGT TGCAGCTGCA AACCGACTTA 
CTTGAGGAAC TAGATGTAAT AAATGGTACT GCAGAACTTC TTGAAGGAGA TCATCGGTCC 
TCATCAGAGG AGAAGGAAGA GAAAGCTTTT CCTACTGTTG GAGCTCAGTC TTCAGTACCA 
GTTGGTGTCA CAGAGTCTGA CATGACTGTA CCAGAAGAAT CTCAAAGAGC TGGACCTACC 
CAGGTGCAGA GCAAGCCTAC TATGCTGAAT GAACCTGATG ATTTGGAAGC AGAGATGTTG 
GAAGAAACAG AAGATAAAAA GCACCAGAGT AAGGAATTTC CCATCACTGT TAATGAAGTG 
TGGGTAAAAG AGAAGGAGGA TAAGGCACAA CATGACAGGA GAAACGAGGC AGCTTCTGAG 
AAACTAGTGC TTGACAAGGT GCCACCACAA AAGGAAGCTC CAGAAGAAAC TTGTTCCAGT 
GACTCTCTGC AACCTGAACC CTTATTCCAG AGGGTAATAC AGCCCACAGC TGTACCAACA 
GCCTCACCAG CTCTGTCAGC TCAGTCTTCA CAGGAAGAGC CTTTTGTACC TCGTTCACGT 
TCCATATCAC CAGCAAAAAA TAAAGGCAAA AGTTCATTGT TGATTGGACT TTCTACAGGG 
CTTTTTGATG CAAACGACCC AAAGATGTTG AGAACTTGTT CGCTCCCAGA TCTTTACAAA 
CTGTACAGAA CTTTAGTTGA TGTCCCCAGT GTAGCAGATG TGCAGCATCA ACATAATCTT 
GAAATAGATG ATACAGAAGA TGAGCAAGCC AAAGAAGGAC CCTCTGATTC TGAGGATATT 
ATGTTTGGGG AGGCAGATAC AGATTTGCAG GAGCTCCGGG CCTCAATGGA ACAATTGCTT 
AGTGAGCAGC CTAGTGAGGA ATTCAGTGAA GAGGAGGAGT CTACTTTAAA GGCTAATGTC 
ATCGAATGCA TAACAAATGG TACAGAGCTG GATGAAGGAG ACGAAAATAA CCCCAGCAGT 
GAAAGTGCAC TTAATGAAGA ATGGCAGTCA GATAATAGTG ATGGAGAGAT TGCCAGTGAA 
TGTGAAGAAT GTGATAGTAT TTTCAGCCAT TTGGAGGAGT TGAGATACAA CCTTGAGCGG 
GAAATAGGCT TTGATAAATT CATTGAGGTT TATGAGAAAA TAAAGGCTAT ACATGAAGAT 
GAAGATGAAA ATATTGATAT TTGTTCAACC ATTGTTCAGA CTATTCTTGG AAATGAACAC 
AAACACCTGT ATGCCAAAAT ACTTCACCTA GTAATGGCAG ACGGAGCCTA TCAAGAAGAT 
AACGATGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578843.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577145.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OAq17594
Clone ID Related Accession (Same CDS sequence)	XM_011578843.1
Accession Version	XM_011578843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGGCACCA 
TATTATGGCG GTGGAGGTGG TGTTGGTCCT TTTCCTGTGT CTTCCAGAGG ACAATATGAA 
CACTATCATG CTATTTTTGA CCAGATGCAA CAGCAAAAGG AAAACATTAG AGTAGCTGAA 
GAGAGGGAAG CCAAATTGAG GGCGAAAGTA CAAGGCCGAG AAGTTGTTGA AAGGCAAAAA 
GGATGGATAG CTGCAGATAG AGCAAAGCAA GTTGAAGAAT TCTGGCAACG AAAGAGAGAA 
GCCATGGAAA ACAAGGCCCG AGCTGAGGGA CACATGGAAT ATTTGGCAAG ATTAAGACAG 
ATCCGGCTAC AAAACTTTAA TGAACGTCAA CAGATTAGAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTG CCAGTTCTGA TCGACAAGAA TCAAGTGAGG AAGCAGAACT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GAAAGGAAGC ATATGAAAGA GAGAAAAGAG CCTGGGAAGA ACATCTGATA 
GCGAAAGGGG TAAAGGATCC TAATGTGGGA GCTACAGAAC AGAGTCCTGT GCATGGGCTA 
GAAGAGCTTG GAGCAGCTCT TCCTAAGCCA CAAGTTCCAG TGAAACCTGG TACACCAGTC 
ATCTCCATGA CTTCTGCTTT GAAGGAAGTG GGTGTGGATG AAAATGTAAC TGCTATCCAG 
GAAGCTGAGG AGGAAATAAA AAAGCCAGAT TTTGCAATGC AAAACAAACG AGAAATACTG 
AGAAGATTAA ATCAAAATCT TAAAGCTCAA GAAGATGAGA AAGGGAAAAA GGAGTGTAAA 
AACATCTCTG AATTGGCTGG CTCTGAAGAT GGTAAGGAAC AGCACGAAGG TGACCATCCA 
CTTCTAGGAG ATCGCAAAAA ATGGGAATCT GGGGCATCTG AGTTGGTAGT TCCTCTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGTC AAACTCTGGG GGAAGTAATT 
AGGTTAGATA TTGGTGAACC CCACAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCAGTC 
CTGAAGATCC TAGGAGAAGC AGAGTTGCAG CTGCAAACCG ACTTACTTGA GGAACTAGAT 
GTAATAAATG GTACTGCAGA ACTTCTTGAA GGAGATCATC GGTCCTCATC AGAGGAGAAG 
GAAGAGAAAG CTTTTCCTAC TGTTGGAGCT CAGTCTTCAG TACCAGTTGG TGTCACAGAG 
TCTGACATGA CTGTACCAGA AGAATCTCAA AGAGCTGGAC CTACCCAGGT GCAGAGCAAG 
CCTACTATGC TGAATGAACC TGATGATTTG GAAGCAGAGA TGTTGGAAGA AACAGAAGAT 
AAAAAGCACC AGAGTAAGGA ATTTCCCATC ACTGTTAATG AAGTGTGGGT AAAAGAGAAG 
GAGGATAAGG CACAACATGA CAGGAGAAAC GAGGCAGCTT CTGAGAAACT AGTGCTTGAC 
AAGGTGCCAC CACAAAAGGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCCTTAT TCCAGAGGGT AATACAGCCC ACAGCTGTAC CAACAGCCTC ACCAGCTCTG 
TCAGCTCAGT CTTCACAGGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCACCAGCA 
AAAAATAAAG GCAAAAGTTC ATTGTTGATT GGACTTTCTA CAGGGCTTTT TGATGCAAAC 
GACCCAAAGA TGTTGAGAAC TTGTTCGCTC CCAGATCTTT ACAAACTGTA CAGAACTTTA 
GTTGATGTCC CCAGTGTAGC AGATGTGCAG CATCAACATA ATCTTGAAAT AGATGATACA 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATTATGTT TGGGGAGGCA 
GATACAGATT TGCAGGAGCT CCGGGCCTCA ATGGAACAAT TGCTTAGTGA GCAGCCTAGT 
GAGGAATTCA GTGAAGAGGA GGAGTCTACT TTAAAGGCTA ATGTCATCGA ATGCATAACA 
AATGGTACAG AGCTGGATGA AGGAGACGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GAGATTGCCA GTGAATGTGA AGAATGTGAT 
AGTATTTTCA GCCATTTGGA GGAGTTGAGA TACAACCTTG AGCGGGAAAT AGGCTTTGAT 
AAATTCATTG AGGTTTATGA GAAAATAAAG GCTATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAACCATTGT TCAGACTATT CTTGGAAATG AACACAAACA CCTGTATGCC 
AAAATACTTC ACCTAGTAAT GGCAGACGGA GCCTATCAAG AAGATAACGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577145.1
CDS	74..3610
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578843.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578843.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGGCACCA 
TATTATGGCG GTGGAGGTGG TGTTGGTCCT TTTCCTGTGT CTTCCAGAGG ACAATATGAA 
CACTATCATG CTATTTTTGA CCAGATGCAA CAGCAAAAGG AAAACATTAG AGTAGCTGAA 
GAGAGGGAAG CCAAATTGAG GGCGAAAGTA CAAGGCCGAG AAGTTGTTGA AAGGCAAAAA 
GGATGGATAG CTGCAGATAG AGCAAAGCAA GTTGAAGAAT TCTGGCAACG AAAGAGAGAA 
GCCATGGAAA ACAAGGCCCG AGCTGAGGGA CACATGGAAT ATTTGGCAAG ATTAAGACAG 
ATCCGGCTAC AAAACTTTAA TGAACGTCAA CAGATTAGAG CAAAACTTCG TGGAGAGAAG 
AATGAAGCTG CCAGTTCTGA TCGACAAGAA TCAAGTGAGG AAGCAGAACT GAAACGCAAA 
AAGATAGAAG CACAGAAGGC CCAAGCAAAT GCTCGAGCTG CAGTTCTGAA AGAACAGTTA 
GAGCGAAAGA GAAAGGAAGC ATATGAAAGA GAGAAAAGAG CCTGGGAAGA ACATCTGATA 
GCGAAAGGGG TAAAGGATCC TAATGTGGGA GCTACAGAAC AGAGTCCTGT GCATGGGCTA 
GAAGAGCTTG GAGCAGCTCT TCCTAAGCCA CAAGTTCCAG TGAAACCTGG TACACCAGTC 
ATCTCCATGA CTTCTGCTTT GAAGGAAGTG GGTGTGGATG AAAATGTAAC TGCTATCCAG 
GAAGCTGAGG AGGAAATAAA AAAGCCAGAT TTTGCAATGC AAAACAAACG AGAAATACTG 
AGAAGATTAA ATCAAAATCT TAAAGCTCAA GAAGATGAGA AAGGGAAAAA GGAGTGTAAA 
AACATCTCTG AATTGGCTGG CTCTGAAGAT GGTAAGGAAC AGCACGAAGG TGACCATCCA 
CTTCTAGGAG ATCGCAAAAA ATGGGAATCT GGGGCATCTG AGTTGGTAGT TCCTCTAGCT 
CAGTTATCAA TGGAAGATTC TCTCTCTGGA ACAGACCGTC AAACTCTGGG GGAAGTAATT 
AGGTTAGATA TTGGTGAACC CCACAGGAAA GTCTGGGGCA AAAGCCCTAC TGATTCAGTC 
CTGAAGATCC TAGGAGAAGC AGAGTTGCAG CTGCAAACCG ACTTACTTGA GGAACTAGAT 
GTAATAAATG GTACTGCAGA ACTTCTTGAA GGAGATCATC GGTCCTCATC AGAGGAGAAG 
GAAGAGAAAG CTTTTCCTAC TGTTGGAGCT CAGTCTTCAG TACCAGTTGG TGTCACAGAG 
TCTGACATGA CTGTACCAGA AGAATCTCAA AGAGCTGGAC CTACCCAGGT GCAGAGCAAG 
CCTACTATGC TGAATGAACC TGATGATTTG GAAGCAGAGA TGTTGGAAGA AACAGAAGAT 
AAAAAGCACC AGAGTAAGGA ATTTCCCATC ACTGTTAATG AAGTGTGGGT AAAAGAGAAG 
GAGGATAAGG CACAACATGA CAGGAGAAAC GAGGCAGCTT CTGAGAAACT AGTGCTTGAC 
AAGGTGCCAC CACAAAAGGA AGCTCCAGAA GAAACTTGTT CCAGTGACTC TCTGCAACCT 
GAACCCTTAT TCCAGAGGGT AATACAGCCC ACAGCTGTAC CAACAGCCTC ACCAGCTCTG 
TCAGCTCAGT CTTCACAGGA AGAGCCTTTT GTACCTCGTT CACGTTCCAT ATCACCAGCA 
AAAAATAAAG GCAAAAGTTC ATTGTTGATT GGACTTTCTA CAGGGCTTTT TGATGCAAAC 
GACCCAAAGA TGTTGAGAAC TTGTTCGCTC CCAGATCTTT ACAAACTGTA CAGAACTTTA 
GTTGATGTCC CCAGTGTAGC AGATGTGCAG CATCAACATA ATCTTGAAAT AGATGATACA 
GAAGATGAGC AAGCCAAAGA AGGACCCTCT GATTCTGAGG ATATTATGTT TGGGGAGGCA 
GATACAGATT TGCAGGAGCT CCGGGCCTCA ATGGAACAAT TGCTTAGTGA GCAGCCTAGT 
GAGGAATTCA GTGAAGAGGA GGAGTCTACT TTAAAGGCTA ATGTCATCGA ATGCATAACA 
AATGGTACAG AGCTGGATGA AGGAGACGAA AATAACCCCA GCAGTGAAAG TGCACTTAAT 
GAAGAATGGC AGTCAGATAA TAGTGATGGA GAGATTGCCA GTGAATGTGA AGAATGTGAT 
AGTATTTTCA GCCATTTGGA GGAGTTGAGA TACAACCTTG AGCGGGAAAT AGGCTTTGAT 
AAATTCATTG AGGTTTATGA GAAAATAAAG GCTATACATG AAGATGAAGA TGAAAATATT 
GATATTTGTT CAACCATTGT TCAGACTATT CTTGGAAATG AACACAAACA CCTGTATGCC 
AAAATACTTC ACCTAGTAAT GGCAGACGGA GCCTATCAAG AAGATAACGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011578844.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011577146.1 Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OAq17595
Clone ID Related Accession (Same CDS sequence)	XM_011578844.1
Accession Version	XM_011578844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	serine/threonine-protein kinase Nek1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950894.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGCCT TTCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGGTACAC TGCAAAACGT GGCAGATACC TATGGAAGCA GGCCCATTTC TGCAAGAGGA 
AGGAAATCCA GAAACAAGGA GGAAGAGGAA TATTTGGCAA GATTAAGACA GATCCGGCTA 
CAAAACTTTA ATGAACGTCA ACAGATTAGA GCAAAACTTC GTGGAGAGAA GAATGAAGCT 
GCCAGTTCTG ATCGACAAGA ATCAAGTGAG GAAGCAGAAC TGAAACGCAA AAAGATAGAA 
GCACAGAAGG CCCAAGCAAA TGCTCGAGCT GCAGTTCTGA AAGAACAGTT AGAGCGAAAG 
AGAAAGGAAG CATATGAAAG AGAGAAAAGA GCCTGGGAAG AACATCTGAT AGCGAAAGGG 
GTAAAGGATC CTAATGTGGG AGCTACAGAA CAGAGTCCTG TGCATGGGCT AGAAGAGCTT 
GGAGCAGCTC TTCCTAAGCC ACAAGTTCCA GTGAAACCTG GTACACCAGT CATCTCCATG 
ACTTCTGCTT TGAAGGAAGT GGGTGTGGAT GAAAATGTAA CTGCTATCCA GGAAGCTGAG 
GAGGAAATAA AAAAGCCAGA TTTTGCAATG CAAAACAAAC GAGAAATACT GAGAAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAG AAAGGGAAAA AGGAGTGTAA AAACATCTCT 
GAATTGGCTG GCTCTGAAGA TGGTAAGGAA CAGCACGAAG GTGACCATCC ACTTCTAGGA 
GATCGCAAAA AATGGGAATC TGGGGCATCT GAGTTGGTAG TTCCTCTAGC TCAGTTATCA 
ATGGAAGATT CTCTCTCTGG AACAGACCGT CAAACTCTGG GGGAAGTAAT TAGGTTAGAT 
ATTGGTGAAC CCCACAGGAA AGTCTGGGGC AAAAGCCCTA CTGATTCAGT CCTGAAGATC 
CTAGGAGAAG CAGAGTTGCA GCTGCAAACC GACTTACTTG AGGAACTAGA TGTAATAAAT 
GGTACTGCAG AACTTCTTGA AGGAGATCAT CGGTCCTCAT CAGAGGAGAA GGAAGAGAAA 
GCTTTTCCTA CTGTTGGAGC TCAGTCTTCA GTACCAGTTG GTGTCACAGA GTCTGACATG 
ACTGTACCAG AAGAATCTCA AAGAGCTGGA CCTACCCAGG TGCAGAGCAA GCCTACTATG 
CTGAATGAAC CTGATGATTT GGAAGCAGAG ATGTTGGAAG AAACAGAAGA TAAAAAGCAC 
CAGAGTAAGG AATTTCCCAT CACTGTTAAT GAAGTGTGGG TAAAAGAGAA GGAGGATAAG 
GCACAACATG ACAGGAGAAA CGAGGCAGCT TCTGAGAAAC TAGTGCTTGA CAAGGTGCCA 
CCACAAAAGG AAGCTCCAGA AGAAACTTGT TCCAGTGACT CTCTGCAACC TGAACCCTTA 
TTCCAGAGGG TAATACAGCC CACAGCTGTA CCAACAGCCT CACCAGCTCT GTCAGCTCAG 
TCTTCACAGG AAGAGCCTTT TGTACCTCGT TCACGTTCCA TATCACCAGC AAAAAATAAA 
GGCAAAAGTT CATTGTTGAT TGGACTTTCT ACAGGGCTTT TTGATGCAAA CGACCCAAAG 
ATGTTGAGAA CTTGTTCGCT CCCAGATCTT TACAAACTGT ACAGAACTTT AGTTGATGTC 
CCCAGTGTAG CAGATGTGCA GCATCAACAT AATCTTGAAA TAGATGATAC AGAAGATGAG 
CAAGCCAAAG AAGGACCCTC TGATTCTGAG GATATTATGT TTGGGGAGGC AGATACAGAT 
TTGCAGGAGC TCCGGGCCTC AATGGAACAA TTGCTTAGTG AGCAGCCTAG TGAGGAATTC 
AGTGAAGAGG AGGAGTCTAC TTTAAAGGCT AATGTCATCG AATGCATAAC AAATGGTACA 
GAGCTGGATG AAGGAGACGA AAATAACCCC AGCAGTGAAA GTGCACTTAA TGAAGAATGG 
CAGTCAGATA ATAGTGATGG AGAGATTGCC AGTGAATGTG AAGAATGTGA TAGTATTTTC 
AGCCATTTGG AGGAGTTGAG ATACAACCTT GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
GAGGTTTATG AGAAAATAAA GGCTATACAT GAAGATGAAG ATGAAAATAT TGATATTTGT 
TCAACCATTG TTCAGACTAT TCTTGGAAAT GAACACAAAC ACCTGTATGC CAAAATACTT 
CACCTAGTAA TGGCAGACGG AGCCTATCAA GAAGATAACG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011577146.1
CDS	74..3961
Translation	MDKYIKVRKIGEGSFGKAILVKAKENGQQYVIKEINISKMSNKEREESRREVAVLANMKHPNIVLYRESFEENGCLYIVMDYCEGGDLFKKINAQKGILFSEDQILDWFVQICLALKHIHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTAELARTCIGTPYYLSPEICQNKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGPFPPVSVHYSYDLRNLLSQLFKRNPRNRPSVNSILEKNFIAKRVEKFLTPQLIAEEFNHKIFHKFGPHAAPAKRPAQEQIMTSVAPAQKITKPAAKYGVPLVIRKSCDAPKKPNEKKPLAKSRQAFPTPMKKMNPGEERRKMFEEPSKKKRLELPEKEKRQRDQISLLKADEMRRLEKERMERINRAREQGWRNVLGSGGSGDVKAPYYGGGGGVGPFPVSSRGQYEHYHAIFDQMQQQKENIRVAEEREAKLRAKVQGREVVERGIPPGIRPGVPKGPAGHHHLPDAGAVRKKMKRLKEVSKQANANRQKGWIAADRAKQVEEFWQRKREAMENKARAEGHMGTLQNVADTYGSRPISARGRKSRNKEEEEYLARLRQIRLQNFNERQQIRAKLRGEKNEAASSDRQESSEEAELKRKKIEAQKAQANARAAVLKEQLERKRKEAYEREKRAWEEHLIAKGVKDPNVGATEQSPVHGLEELGAALPKPQVPVKPGTPVISMTSALKEVGVDENVTAIQEAEEEIKKPDFAMQNKREILRRLNQNLKAQEDEKGKKECKNISELAGSEDGKEQHEGDHPLLGDRKKWESGASELVVPLAQLSMEDSLSGTDRQTLGEVIRLDIGEPHRKVWGKSPTDSVLKILGEAELQLQTDLLEELDVINGTAELLEGDHRSSSEEKEEKAFPTVGAQSSVPVGVTESDMTVPEESQRAGPTQVQSKPTMLNEPDDLEAEMLEETEDKKHQSKEFPITVNEVWVKEKEDKAQHDRRNEAASEKLVLDKVPPQKEAPEETCSSDSLQPEPLFQRVIQPTAVPTASPALSAQSSQEEPFVPRSRSISPAKNKGKSSLLIGLSTGLFDANDPKMLRTCSLPDLYKLYRTLVDVPSVADVQHQHNLEIDDTEDEQAKEGPSDSEDIMFGEADTDLQELRASMEQLLSEQPSEEFSEEEESTLKANVIECITNGTELDEGDENNPSSESALNEEWQSDNSDGEIASECEECDSIFSHLEELRYNLEREIGFDKFIEVYEKIKAIHEDEDENIDICSTIVQTILGNEHKHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011578844.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis NIMA-related kinase 1 (NEK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011578844.1

ORF Insert Sequence:

ATGGATAAAT ATATTAAAGT GCGAAAGATT GGAGAAGGAT CCTTTGGGAA AGCAATTCTT 
GTCAAAGCTA AAGAAAATGG CCAACAGTAT GTTATTAAAG AAATAAACAT CTCTAAGATG 
TCAAATAAGG AGAGAGAAGA ATCAAGGAGA GAAGTTGCTG TATTGGCTAA CATGAAACAT 
CCAAATATTG TCCTGTATAG GGAGTCATTT GAAGAAAATG GCTGTCTTTA CATAGTGATG 
GATTACTGTG AAGGAGGAGA CCTATTTAAA AAAATAAATG CCCAGAAAGG AATCTTGTTC 
TCTGAAGATC AGATTCTGGA TTGGTTTGTA CAAATCTGTT TAGCACTAAA ACACATACAC 
GATAGAAAAA TCTTGCATCG GGACATAAAG TCACAGAACA TATTTTTAAC CAAAGATGGA 
ACAATACAGC TGGGAGATTT TGGGATCGCC AGAGTTCTTA ACAGTACTGC GGAGTTGGCT 
CGCACTTGCA TAGGAACGCC ATACTACTTG TCACCTGAAA TATGTCAGAA CAAGCCTTAC 
AACAATAAAA GCGATATCTG GGCCCTTGGT TGTGTTCTCT ATGAAATGTG CACACTTAAA 
CATGCGTTTG AAGCTGGCAA CATGAAGAAC TTGGTACTGA AGATAATCTC TGGACCTTTT 
CCTCCTGTTT CTGTGCATTA CTCCTATGAC CTACGTAATC TGCTGTCACA GTTATTTAAA 
AGGAATCCTA GGAATAGACC ATCAGTAAAC TCCATATTGG AGAAAAACTT TATAGCCAAA 
CGGGTTGAAA AATTTCTCAC ACCACAGCTT ATTGCAGAAG AATTCAATCA CAAAATCTTC 
CATAAGTTTG GACCACATGC TGCACCGGCT AAAAGACCAG CTCAAGAACA AATAATGACT 
TCAGTTGCAC CAGCTCAGAA AATTACCAAA CCTGCTGCAA AATATGGAGT GCCTTTAGTA 
ATCAGGAAGT CTTGTGATGC ACCTAAGAAG CCTAATGAGA AGAAACCATT GGCTAAATCT 
AGACAGGCCT TTCCAACTCC AATGAAGAAA ATGAATCCAG GAGAAGAAAG GAGGAAAATG 
TTTGAGGAAC CTTCAAAAAA GAAAAGGTTA GAATTGCCTG AAAAAGAAAA ACGCCAGAGA 
GATCAGATCA GTTTACTGAA GGCAGATGAA ATGAGAAGAT TGGAAAAAGA AAGGATGGAA 
CGAATAAACA GAGCCAGGGA ACAAGGATGG AGGAATGTGC TTGGTTCAGG TGGAAGTGGT 
GATGTTAAGG CACCATATTA TGGCGGTGGA GGTGGTGTTG GTCCTTTTCC TGTGTCTTCC 
AGAGGACAAT ATGAACACTA TCATGCTATT TTTGACCAGA TGCAACAGCA AAAGGAAAAC 
ATTAGAGTAG CTGAAGAGAG GGAAGCCAAA TTGAGGGCGA AAGTACAAGG CCGAGAAGTT 
GTTGAAAGAG GAATTCCACC TGGAATACGT CCAGGAGTTC CTAAGGGGCC TGCAGGTCAT 
CACCATTTAC CTGATGCTGG TGCAGTTAGG AAAAAAATGA AAAGATTGAA GGAGGTGTCT 
AAACAAGCCA ATGCAAACAG GCAAAAAGGA TGGATAGCTG CAGATAGAGC AAAGCAAGTT 
GAAGAATTCT GGCAACGAAA GAGAGAAGCC ATGGAAAACA AGGCCCGAGC TGAGGGACAC 
ATGGGTACAC TGCAAAACGT GGCAGATACC TATGGAAGCA GGCCCATTTC TGCAAGAGGA 
AGGAAATCCA GAAACAAGGA GGAAGAGGAA TATTTGGCAA GATTAAGACA GATCCGGCTA 
CAAAACTTTA ATGAACGTCA ACAGATTAGA GCAAAACTTC GTGGAGAGAA GAATGAAGCT 
GCCAGTTCTG ATCGACAAGA ATCAAGTGAG GAAGCAGAAC TGAAACGCAA AAAGATAGAA 
GCACAGAAGG CCCAAGCAAA TGCTCGAGCT GCAGTTCTGA AAGAACAGTT AGAGCGAAAG 
AGAAAGGAAG CATATGAAAG AGAGAAAAGA GCCTGGGAAG AACATCTGAT AGCGAAAGGG 
GTAAAGGATC CTAATGTGGG AGCTACAGAA CAGAGTCCTG TGCATGGGCT AGAAGAGCTT 
GGAGCAGCTC TTCCTAAGCC ACAAGTTCCA GTGAAACCTG GTACACCAGT CATCTCCATG 
ACTTCTGCTT TGAAGGAAGT GGGTGTGGAT GAAAATGTAA CTGCTATCCA GGAAGCTGAG 
GAGGAAATAA AAAAGCCAGA TTTTGCAATG CAAAACAAAC GAGAAATACT GAGAAGATTA 
AATCAAAATC TTAAAGCTCA AGAAGATGAG AAAGGGAAAA AGGAGTGTAA AAACATCTCT 
GAATTGGCTG GCTCTGAAGA TGGTAAGGAA CAGCACGAAG GTGACCATCC ACTTCTAGGA 
GATCGCAAAA AATGGGAATC TGGGGCATCT GAGTTGGTAG TTCCTCTAGC TCAGTTATCA 
ATGGAAGATT CTCTCTCTGG AACAGACCGT CAAACTCTGG GGGAAGTAAT TAGGTTAGAT 
ATTGGTGAAC CCCACAGGAA AGTCTGGGGC AAAAGCCCTA CTGATTCAGT CCTGAAGATC 
CTAGGAGAAG CAGAGTTGCA GCTGCAAACC GACTTACTTG AGGAACTAGA TGTAATAAAT 
GGTACTGCAG AACTTCTTGA AGGAGATCAT CGGTCCTCAT CAGAGGAGAA GGAAGAGAAA 
GCTTTTCCTA CTGTTGGAGC TCAGTCTTCA GTACCAGTTG GTGTCACAGA GTCTGACATG 
ACTGTACCAG AAGAATCTCA AAGAGCTGGA CCTACCCAGG TGCAGAGCAA GCCTACTATG 
CTGAATGAAC CTGATGATTT GGAAGCAGAG ATGTTGGAAG AAACAGAAGA TAAAAAGCAC 
CAGAGTAAGG AATTTCCCAT CACTGTTAAT GAAGTGTGGG TAAAAGAGAA GGAGGATAAG 
GCACAACATG ACAGGAGAAA CGAGGCAGCT TCTGAGAAAC TAGTGCTTGA CAAGGTGCCA 
CCACAAAAGG AAGCTCCAGA AGAAACTTGT TCCAGTGACT CTCTGCAACC TGAACCCTTA 
TTCCAGAGGG TAATACAGCC CACAGCTGTA CCAACAGCCT CACCAGCTCT GTCAGCTCAG 
TCTTCACAGG AAGAGCCTTT TGTACCTCGT TCACGTTCCA TATCACCAGC AAAAAATAAA 
GGCAAAAGTT CATTGTTGAT TGGACTTTCT ACAGGGCTTT TTGATGCAAA CGACCCAAAG 
ATGTTGAGAA CTTGTTCGCT CCCAGATCTT TACAAACTGT ACAGAACTTT AGTTGATGTC 
CCCAGTGTAG CAGATGTGCA GCATCAACAT AATCTTGAAA TAGATGATAC AGAAGATGAG 
CAAGCCAAAG AAGGACCCTC TGATTCTGAG GATATTATGT TTGGGGAGGC AGATACAGAT 
TTGCAGGAGC TCCGGGCCTC AATGGAACAA TTGCTTAGTG AGCAGCCTAG TGAGGAATTC 
AGTGAAGAGG AGGAGTCTAC TTTAAAGGCT AATGTCATCG AATGCATAAC AAATGGTACA 
GAGCTGGATG AAGGAGACGA AAATAACCCC AGCAGTGAAA GTGCACTTAA TGAAGAATGG 
CAGTCAGATA ATAGTGATGG AGAGATTGCC AGTGAATGTG AAGAATGTGA TAGTATTTTC 
AGCCATTTGG AGGAGTTGAG ATACAACCTT GAGCGGGAAA TAGGCTTTGA TAAATTCATT 
GAGGTTTATG AGAAAATAAA GGCTATACAT GAAGATGAAG ATGAAAATAT TGATATTTGT 
TCAACCATTG TTCAGACTAT TCTTGGAAAT GAACACAAAC ACCTGTATGC CAAAATACTT 
CACCTAGTAA TGGCAGACGG AGCCTATCAA GAAGATAACG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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[email protected]

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

NEK1 cDNA ORF clone, Aquila chrysaetos canadensis

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム